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effective chemical control of psychrotrophic bacillus cereus epso-35as and inra tz415 spore outgrowth in carrot broth.the growth kinetic parameters of germinated cells from heat-activated spores of the psychrotrophic bacillus cereus epso-35as strain in nutrient broth (nb) and in tyndallized carrot broth (tcb) were evaluated at different temperatures (8, 12, and 16 degrees c) for control samples and for samples acidified with citric acid or lemon juice at ph values between 4.7 and 5.5. lowering the ph from 7.4 or 6.2 to 5.2 inhibited bacterial growth in both tested media after 60 days at 12 degrees c and lower t ...200818541171
the deposition of bacterial cells from laminar flows onto solid surfaces.in a previous article, the authors proposed a simple model for the rate of removal of bacterial cells from solid surfaces by fluid shear. this model has been extended to include the deposition of cells from a flowing suspension. the theory is compared to experimentally obtained data for the deposition of bacillus cereus cells onto the surface of glass capillaries. the effect of a hydrophobic surface, siliconized glass, and the addition of an antifoam agent to the suspension is also investigated.198318548707
a bacillus thuringiensis strain producing a polyglutamate capsule resembling that of bacillus anthracis.bacillus thuringiensis serovar monterrey strain bgsc 4aj1 produced a microscopically visible capsule that reacted with a fluorescent antibody specific for the poly-gamma-d-glutamic acid (pga) capsule of bacillus anthracis. pga capsule biosynthesis genes with 75%, 81%, 72%, 65% and 63% similarity, respectively, to those of the b. anthracis capbcade cluster were present on a plasmid (paj1-1). strain bgsc 4aj1, together with five strains of bacillus cereus that hybridized to a pga cap gene probe, w ...200818549401
the 1.25 a resolution structure of phosphoribosyl-atp pyrophosphohydrolase from mycobacterium tuberculosis.phosphoribosyl-atp pyrophosphohydrolase is the second enzyme in the histidine-biosynthetic pathway, irreversibly hydrolyzing phosphoribosyl-atp to phosphoribosyl-amp and pyrophosphate. it is encoded by the hise gene, which is present as a separate gene in many bacteria and archaea but is fused to hisi in other bacteria, fungi and plants. because of its essentiality for growth in vitro, hise is a potential drug target for tuberculosis. the crystal structures of two native (uncomplexed) forms of h ...200818560150
saccharides cross-reactive with bacillus anthracis spore glycoprotein as an anthrax vaccine component.bacillus anthracis is a spore-forming bacterium that causes anthrax in humans and in other mammals. the glycoprotein bcla (bacillus collagen-like protein of anthracis) is a major constituent of the exosporium, the outermost surface of b. anthracis spores. the glycosyl part of bcla is an oligosaccharide composed of 2-o-methyl-4-(3-hydroxy-3-methylbutanamido)-4,6-dideoxy-d-glucose, referred to as anthrose, and three rhamnose residues. a structure similar to anthrose, 4-(3-hydroxy-3-methylbutanamid ...200818562275
evaluation and control of the risk of foodborne pathogens and spoilage bacteria present in awa-uirou, a sticky rice cake containing sweet red bean paste.the risk of food poisoning and growth of spoilage bacteria in awa-uirou, a sticky rice cake containing sweet red bean paste, was evaluated. toxin-producing bacteria such as staphylococcus aureus and bacillus cereus are the main causes of food poisoning linked to this kind of food. the water activity in this product is in the range suitable for growth of s. aureus, b. cereus, and b. subtilis. the viable count of s. aureus or b. cereus spore cocktail was significantly reduced to 2.3 log colony-for ...200818564913
challenge testing the lactoperoxidase system against a range of bacteria using different activation agents.lactoperoxidase (lp) exerts antimicrobial effects in combination with h(2)o(2) and either thiocyanate (scn(-)) or a halide (e.g., i(-)). garlic extract in the presence of ethanol has also been used to activate the lp system. this study aimed to determine the effects of 3 lp activation systems (lp+scn(-)+h(2)o(2); lp+i(-)+h(2)o(2); lp + garlic extract + ethanol) on the growth and activity of 3 test organisms (staphylococcus aureus, pseudomonas aeruginosa, and bacillus cereus). sterilized milk was ...200818565914
antagonism between bacillus cereus and pseudomonas fluorescens in planktonic systems and in biofilms.in the environment, many microorganisms coexist in communities competing for resources, and they are often associated as biofilms. the investigation of bacterial ecology and interactions may help to improve understanding of the ability of biofilms to persist. in this study, the behaviour of bacillus cereus and pseudomonas fluorescens in the planktonic and sessile states was compared. planktonic tests were performed with single and dual species cultures in growth medium with and without supplemen ...200818576180
single-chain variable fragment antibodies selected by phage display against the sporozoite surface antigen p23 of cryptosporidium parvum.cryptosporidium parvum, an apicomplexan parasite transmitted via animal fecal wastes, is the causative agent of cryptosporidiosis. clones were selected from 2 synthetic naïve human single-chain variable fragment (scfv) phagemid libraries that bound to the recombinant p23 protein of c. parvum. panning the tomlinson i and j phagemid libraries resulted in 6 distinct clones. two clones had full-length scfv sequences, while the remaining clones were either truncated or missing a section of the heavy ...200918576885
antibacterial activity of labdane diterpenoids from stemodia foliosa.as part of a continuing interest in exploring the chemistry of brazilian medicinal plants, three new labdane diterpenoids, 6alpha-acetoxymanoyl oxide (1), 6alpha-malonyloxymanoyl oxide (2), and 6alpha-malonyloxy-n-butyl ester manoyl oxide (3), together with the known betulinic acid, lupeol, sitosterol, and stigmasterol, were isolated from the aerial parts of stemodia foliosa. the structures of 1-3 were established on the basis of interpretation of spectroscopic data, including hresims, and 1d an ...200818582112
screening of some tanzanian medicinal plants from bunda district for antibacterial, antifungal and antiviral activities.extracts from 50 plant parts obtained from 39 different plants belonging to 22 families used to treat infectious diseases in bunda district, tanzania, were screened against twelve microorganisms, including the bacteria bacillus cereus, staphylococcus aureus, escherichia coli, pseudomonas aeruginosa, klebsiella pneumoniae and salmonella typhimurium, the fungi aspergillus niger, candida albicans, and the viruses herpes simplex virus type 1, vesicular stomatitis virus t2, coxsackie b2 and semliki f ...200818582554
evaluation of the plasmid copy number in b. cereus spores, during germination, bacterial growth and sporulation using real-time pcr.only a small number of studies have measured the plasmid copy number (pcn) variation during bacterial growth. besides, information about the pcn in spores is still rare. in this work, we utilized a real-time pcr assay to evaluate the pcn of four different plasmids in bacillus cereus. the pcn was measured in spores as well as during germination, active bacterial growth, and sporulation. plasmid stability was also evaluated to ensure that plasmid loss does not affect the accuracy of the pcn measur ...200818582938
a new protein architecture for processing alkylation damaged dna: the crystal structure of dna glycosylase alkd.dna glycosylases safeguard the genome by locating and excising chemically modified bases from dna. alkd is a recently discovered bacterial dna glycosylase that removes positively charged methylpurines from dna, and was predicted to adopt a protein fold distinct from from those of other dna repair proteins. the crystal structure of bacillus cereus alkd presented here shows that the protein is composed exclusively of helical heat-like repeats, which form a solenoid perfectly shaped to accommodate ...200818585735
a role for tumor necrosis factor-alpha in experimental bacillus cereus endophthalmitis pathogenesis.to determine the contribution of tumor necrosis factor-alpha (tnfalpha) in the pathogenesis of experimental bacillus cereus endophthalmitis.200818586878
survey of group i and group ii introns in 29 sequenced genomes of the bacillus cereus group: insights into their spread and evolution.group i and group ii introns are different catalytic self-splicing and mobile rna elements that contribute to genome dynamics. in this study, we have analyzed their distribution and evolution in 29 sequenced genomes from the bacillus cereus group of bacteria. introns were of different structural classes and evolutionary origins, and a large number of nearly identical elements are shared between multiple strains of different sources, suggesting recent lateral transfers and/or that introns are und ...200818587153
[cloning and characterization of ectabc cluster from bacillus alcalophilus dty1].bacillus alcalophilus dty1, one moderate halophytic bacterium isolated from saline soil in loess plateau of china, was characterized with efficient production of ectoine. in this study, the gene cluster ectabc taking in charge of biosynthesizing ectoine was cloned from the genomic library of strain dty1. nucleotide sequencing indicated that ecta, ectb and ectc were predicted to encode peptides of 169, 428 and 132 amino acids, respectively. the deduced amino acid sequences of ecta, ectb and ectc ...200818589814
levels and toxigenicity of bacillus cereus and clostridium perfringens from retail seafood.for the period 1990 through 2003, seafood was the most commonly identified food linked to foodborne outbreaks in the united states. fish as a commodity has rarely been examined for the presence of bacillus cereus in particular. for the present study, 347 fresh and processed retail seafood samples were examined for the presence of clostridium botulinum, clostridium perfringens, and b. cereus. the presence of c. botulinum was not confirmed in any of the isolates, but c. perfringens was confirmed i ...200818592743
[markers for species-specific detection of bacteria from bacillus cereus group].rpob and gyr genes (and their fragments) of chromosomal dna of bacteria from bacillus cereus group - b. anthracis, b. cereus, and b. thuringiensis - which are the potential markers for their genotyping were sequenced and phylogenetic trees were constructed. sets of primers for species-specific detection of b. anthracis, b. cereus, and b. thuringiensis by multiplex polymerase chain reaction were designed. also primers sets, which allow to differentiate strains of b. anthracis with various plasmid ...200818595460
genotyping of bacillus cereus strains by microarray-based resequencing.the ability to distinguish microbial pathogens from closely related but nonpathogenic strains is key to understanding the population biology of these organisms. in this regard, bacillus anthracis, the bacterium that causes inhalational anthrax, is of interest because it is closely related and often difficult to distinguish from other members of the b. cereus group that can cause diverse diseases. we employed custom-designed resequencing arrays (ras) based on the genome sequence of bacillus anthr ...200818596941
riboprint and virulence gene patterns for bacillus cereus and related species.a total of 72 bacillus cereus strains and 5 bacillus thuringiensis strains were analyzed for their ecori ribogroup by ribotyping and for the presence or absence of seven virulence-associated genes. from these 77 strains, 42 distinctive ribogroup were identified using ecori, but the two species could not be discriminated by their ecori ribogroup. the 77 strains were also examined by pcr for the presence of seven virulence-associated genes, cerab, pi-plc, entfm, bcet, hbla, hblc, and hbld. all fiv ...200818600061
risk profiles of pork and poultry meat and risk ratings of various pathogen/product combinations.risk profiles of pork and poultry meat were carried out using an excel-based software program, risk ranger. it is a semi-quantitative risk estimator answering various questions relating to the probability of exposure to a hazard, susceptibility of the population of interest, severity of the illness caused by the hazard if present and probability of food containing an infectious dose. therefore, qualitative and quantitative inputs were used to estimate and rank the risk of various hazards/food co ...200818602180
bacillus cereus nosocomial infection from reused towels in japan.it was noticed that there was an increase in bacillus cereus nosocomial infections in the summer from 2000 to 2005. in 2005, five bloodstream infections occurred in five patients related to catheter use. the causative strains were distinct from each other and belonged to novel multilocus sequence types (st): st365, st366, st367 and st368. two st365 strains from two patients were further distinguished by pulsed-field gel electrophoresis. b. cereus contamination was observed with reused (dried and ...200818602188
real-time pcr system targeting a chromosomal marker specific for bacillus anthracis.specific identification of bacillus anthracis and differentiation from closely related bacillus cereus and bacillus thuringiensis strains is still a major diagnostic problem. commercially available diagnostic kits targeting plasmid-markers cannot differentiate between b. anthracis, non-anthracis bacillus species harbouring anthrax-specific virulence plasmids, and plasmidless b. anthracis strains. a taqman pcr assay was designed targeting sequences of gene locus ba_5345 of the b. anthracis strain ...200818602986
synthesis and anti-microbial screening of some schiff bases of 3-amino-6,8-dibromo-2-phenylquinazolin-4(3h)-ones.in the present study, a series of novel schiff bases were synthesized by condensation of 3-amino-6,8-dibromo-2-phenylquinazolin-4(3h)-ones with different aromatic aldehydes via cyclized intermediate 6,8-dibromo-2-phenyl benzoxazin-4-one. the chemical structures were confirmed by means of ir, (1)h nmr, (13)c nmr, mass spectral and elemental analysis. these compounds were screened for anti-bacterial (staphylococcus aureus atcc-9144, staphylococcus epidermidis atcc-155, micrococcus luteus atcc-4698 ...200918603337
enteric bacteria isolated from acute diarrheal patients in the republic of korea between the year 2004 and 2006.in an epidemiological survey of human enterobacterial infections in the republic of korea during three years from 2004 to 2006, we isolated 1,784 (6.2%, isolation rate of enteropathogens from stool samples) in 2004, 2,547 (9.5%) in 2005 and 3,506 bacteria (12.3%) from people who visited clinics. among the isolated bacteria, pathogenic escherichia coli, especially, eaec was the most frequently identified pathogen in both urban and rural regions followed by staphylococcus aureus, salmonella specie ...200818604503
crystallization and preliminary x-ray crystallographic studies of a pduo-type atp:cob(i)alamin adenosyltransferase from bacillus cereus.cobalamin adenosyltransferases transfer a 5'-deoxyadenosyl moiety from atp and covalently attach it to the cobalt(i) ion of the corrin ring of cobalamin to generate adenosylcobalamin. the pduo-type adenosyltransferase from bacillus cereus was overexpressed in escherichia coli, purified and crystallized as the apoenzyme as well as in complex with mg(2+) and atp (mgatp). diffraction data were collected to 1.9 a resolution for the native crystals and 2.0 a resolution for the complexed crystals. bot ...200818607099
synthesis and antimicrobial studies of novel 1-benzhydryl-piperazine sulfonamide and carboxamide derivatives.a series of novel substituted 1-benzhydryl-piperazine sulfonamide 8(a-f) and benzamides 9(a-h) were synthesized and their antimicrobial activities evaluated in vitro by paper disc diffusion and micro dilution method against standard strains of gram-positive (staphylococcus aureus atcc 25953, staphylococcus epidermis 25212, bacillus cereus 11778, bacillus substilis 6051) and gram-negative (escherichia coli atcc 25922, pseudomonas aeruginosa atcc 2853, proteus vulgaris atcc 2853 and salmonella typ ...200818608768
bacteriocin-producing lactobacillus strains isolated from poto poto, a congolese fermented maize product, and genetic fingerprinting of their plantaricin operons.thirty one bacteriocin-producing lactobacillus isolates were identified among 135 lactobacilli isolated from the congolese fermented maize product poto poto, during the preparation and from the finished product. using species-specific pcr and 16s rrna gene sequencing, 28 and 3 isolates were identified as l. plantarum and l. fermentum, respectively. cluster analysis of rapd-pcr fingerprints revealed two main groups (g1 and g2) plus the l. fermentum isolate c4-13. group g1 contained 23 isolates wi ...200818620772
inhibition of bacillus cereus and bacillus weihenstephanensis in raw vegetables by application of washing solutions containing enterocin as-48 alone and in combination with other antimicrobials.enterocin as-48 is a broad-spectrum cyclic antimicrobial peptide produced by enterococcus faecalis. in the present study, the bacteriocin was tested alone and in combination with other antimicrobials for decontamination of bacillus inoculated on alfalfa, soybean sprouts and green asparagus. washing with enterocin as-48 solutions reduced viable cell counts of bacillus cereus and bacillus weihenstephanensis by 1.0-1.5 and by 1.5-2.38 log units right after application of treatment, respectively. in ...200818620967
amide bonds assemble pili on the surface of bacilli.pilin precursors are the building blocks of pili on the surface of gram-positive bacteria; however, the assembly mechanisms of these adhesive fibers are unknown. here, we describe the chemical bonds that assemble bcpa pilin subunits on the surface of bacillus cereus. sortase d cleaves bcpa precursor between the threonine (t) and the glycine (g) residues of its lpxtg sorting signal and catalyzes formation of an amide bond between threonine (t) of the sorting signal and lysine (k) in the ypkn moti ...200818621716
reduction of pollutants in pulp paper mill effluent treated by pcp-degrading bacterial strains.two pcp-degrading bacterial strains, bacillus cereus (itrc-s6) and serratia marcescens (itrc-s7) were used for the treatment of pulp and paper mill effluent at conditions; 1.0% glucose and 0.5% peptone at 30 +/- 1 degrees c at 120 rpm for 168 h of incubation. these two bacterial strains effectively reduced colour (45-52%), lignin (30-42%), bod (40-70%), cod (50-60%), total phenol (32-40%) and pcp (85-90%) within 168 h of incubation. however, the highest reduction in colour (62%), lignin (54%), b ...200918622710
[analysis of microbial community of a beijing simulator water distribution system].identification of compositions of the biofilm in a beijing simulator water distribution system pipe networks by pcr and single strand conformation polymorphism (pcr-sscp) technique and analysis of its heterotrophic bacterial by heterotrophic plate count (hpc) were performed. results showed that when the water velocities were same, the count of heterotrophic bacterial on the zinficated steel wall was about five times of the pvc. on the other hand, when the pipe materials were zinficated steel, th ...200818624174
environment driven cereulide production by emetic strains of bacillus cereus.the impacts of growth media and temperature on production of cereulide, the emetic toxin of bacillus cereus, were measured for seven well characterised strains selected for diversity of biochemical and genetic properties and sources of origin. all strains carried cereulide synthase gene, ces, on a megaplasmid of ca. 200 kb and all grew up to 48-50 degrees c, but produced cereulide only up to 39 degrees c. on tryptic soy agar five strains, originating from foods, food poisonings and environment, ...200818625533
continuous enzymatic production of xylitol with simultaneous coenzyme regeneration in a charged membrane reactor.we have developed a new process for the production of xylitol from d-xylose by enzyme technology. an nadh-dependent xylose reductase (xr) from candida tenuis catalyzes the reduction of xylose, which is coupled to enzymatic oxidations of d-glucose or d-xylose by glucose dehydrogenase from bacillus cereus to make achievable an up to 10,000-fold regeneration of nadh per cycle of discontinuous conversion. using a simple kinetic model as a tool for process optimization, suitable conditions with regar ...199618629908
riboswitches in eubacteria sense the second messenger cyclic di-gmp.cyclic di-guanosine monophosphate (di-gmp) is a circular rna dinucleotide that functions as a second messenger in diverse species of bacteria to trigger wide-ranging physiological changes, including cell differentiation, conversion between motile and biofilm lifestyles, and virulence gene expression. however, the mechanisms by which cyclic di-gmp regulates gene expression have remained a mystery. we found that cyclic di-gmp in many bacterial species is sensed by a riboswitch class in messenger r ...200818635805
the in vitro pharmacological activities of 12 south african hermannia species.hermannia species are widely used in traditional medicine in southern africa, however no extensive study has been conducted on this genus. the acetone extracts of 12 indigenous hermannia species (flowers, stems and leaves combined) were evaluated for various pharmacological activities. all investigated species displayed promising antimicrobial activity, with hermannia saccifera being the most active against staphylococcus aureus (mic=19.5 microg/ml), bacillus cereus (mic=19.5 microg/ml) and ente ...200818640257
the bacillus cereus gern and gert protein homologs have distinct roles in spore germination and outgrowth, respectively.the gert protein of bacillus cereus shares 74% amino acid identity with its homolog gern. the latter is a na(+)/h(+)-k(+) antiporter that is required for normal spore germination in inosine. the germination properties of single and double mutants of b. cereus atcc 10876 reveal that unlike gern, which is required for all germination responses that involve the geri germinant receptor, the gert protein does not have a significant role in germination, although it is required for the residual geri-me ...200818641133
mitracarpus frigidus aerial parts exhibited potent antimicrobial, antileishmanial, and antioxidant effects.the crude extract and the hexane, ch(2)cl(2), etoac, n-buoh, and hydromethanolic fractions of the aerial parts of mitracarpus frigidus were evaluated against promastigote forms of two species of leishmania (l. chagasi and l. amazonensis), 11 strains of bacteria (staphylococcus aureus, pseudomonas aeruginosa, salmonella enterica sorovar tythimurium, shigella sonnei, klebsiella pneumoniae, escherichia coli, micrococcus luteus, enterococcus faecalis, enterobacter cloacae, streptococcus pyogenes and ...200918644325
in vitro evidence of phyto-synergy for plant part combinations of croton gratissimus (euphorbiaceae) used in african traditional healing.despite the extensive traditional use of croton gratissimus burch. var. gratissimus for medicinal purposes, scientific studies validating the therapeutic properties of this indigenous plant are lacking. as the bark, roots and leaves of c. gratissimus are used separately as well as in combination, this study focused on determining antimicrobial efficacies of the plant parts independently and in combination to assess possible pharmacological interactions (e.g. synergy, antagonism).200818644430
[mechanisms and enzymatic characters of hexavalent chromium reduction by bacillus cereus s5.4].a strain of bacteria identified as bacillus cereus s5.4, which was isolated from the electroplating sludge of baosteel corporation, shanghai, was able to completely reduce 2 mmol/l cr6+ in lb liquid after 72 hours. experiments on concentrations of hexavalent and total chromium inside and outside bacterial cells after hexavalent chromium reduction, and hexavalent chromium reduction abilities of different cellular components, and sem analysis of cellular morphology before and after hexavalent chro ...200818649539
engineered mononuclear variants in bacillus cereus metallo-beta-lactamase bcii are inactive.metallo-beta-lactamases (mbetals) are zinc enzymes able to hydrolyze almost all beta-lactam antibiotics, rendering them inactive, at the same time endowing bacteria high levels of resistance. the design of inhibitors active against all classes of mbetals has been hampered by their structural diversity and by the heterogeneity in metal content in enzymes from different sources. bcii is the metallo-beta-lactamase from bacillus cereus, which is found in both the mononuclear and dinuclear forms. des ...200818652482
enhanced synthesis and antimicrobial activities of bacteriocins produced by mexican strains of bacillus thuringiensis.recently, we reported the synthesis of five bacteriocin-like inhibitor substances (bt-blis: morricin 269, kurstacin 287, kenyacin 404, entomocin 420, and tolworthcin 524) by mexican strains of bacillus thuringiensis. here we show that, collectively, these bt-blis have a moderate to broad spectrum of antibacterial activity, being toxic to clinically significant against gram-positive and gram-negative bacteria, including common etiological agents of human diseases, such as strep throat and scarlet ...200818654760
the response to a specific germinant by bacillus anthracis spores in primary mouse macrophages is modulated by a protein encoded on the pxo1 plasmid.a bacillus anthracis sterne pxo1 plasmid-encoded protein designated cot43 was found in coat extracts of purified spores. cot43 is a tetratricopeptide repeat domain protein related to those which function as phosphatases in the sporulation phosphorelay and as regulators of competence and pathogenic factors. the synthesis of cot43 began in the late exponential phase downstream from a sigmaa promoter (as mapped by race) and it was present at least until the formation of phase white endospores. ther ...200818654763
the identification of response regulator-specific binding sites reveals new roles of two-component systems in bacillus cereus and closely related low-gc gram-positives.in bacteria, environmental challenges are often translated into a transcriptional response via the cognate response regulators (rrs) of specialized two-component systems (tcss). a phylogenetic footprinting/shadowing approach was designed and used to identify many novel rr-specific operators for species of the bacillus cereus group and related gram-positives. analysis of the operator sequences revealed characteristic traits for each rr subfamily. for instance, operators related to the largest sub ...200818662309
food poisoning as a cause of acute liver failure.we report a 9-year-old girl with cereulide-producing bacillus cereus food poisoning, who developed fulminant hepatitis, renal and pancreatic insufficiency, shock, and prolonged seizures. she was transferred to our institution for hepatic transplantation before her diagnosis was established. as a result of rapid identification of the microorganism and supportive care, liver transplantation was avoided, and she recovered fully.200818664929
the plcr virulence regulon of bacillus cereus.plcr is a bacillus cereus transcriptional regulator, which activates gene expression by binding to a nucleotidic sequence called the 'plcr box'. to build a list of all genes included in the plcr regulon, a consensus sequence was identified by directed mutagenesis. the reference strain atcc14579 sequenced genome was searched for occurrences of this consensus sequence to produce a virtual regulon. plcr control of these genes was confirmed by comparing gene expression in the reference strain and it ...200818665214
chemical composition and antimicrobial activities of the essential oil from the seeds of enterolobium contortisiliquum (leguminosae).seeds of enterolobium contortisiliquum were subjected to steam distillation to obtain a light yellow essential oil in a yield of 3 ml/kg of seeds. the major components of the oil were identified using gas chromatography/mass spectrometry (gc-ms) and were furfural, limonene, linalool, estragole, carvone, and apiole with carvone representing more than 50% of the total composition. antimicrobial activities of the essential oil were determined against four species of gram positive bacteria (bacillus ...200818665989
towards on-site pathogen detection using antibody-based sensors.in this paper, the recent progress within biosensors for plant pathogen detection will be reviewed. bio-recognition layers on sensors can be designed in various ways, however the most popular approach is to immobilise antibodies for specific capture of analytes. focus will be put on antibody surface-immobilisation strategies as well as the use of antibodies in the widely used sensors, quartz crystal microbalance, surface plasmon resonance and cantilevers. we will describe the available data on a ...200818675543
neonatal meningoencephalitis caused by bacillus cereus.the classic organisms associated with central nervous system infection in the neonate are herpes simplex, listeria monocytogenes, escherichia coli, and streptococcus agalactiae; we describe an unusual case of neonatal meningoencephalitis caused by bacillus cereus.200818679155
survival of bacillus cereus vegetative cells during spanish-style fermentation of conservolea green olives.the aim of this research was to investigate the survival of artificially inoculated bacillus cereus during spanish-style green olive fermentation. olives were initially treated with lye and subjected to different fermentation procedures including (i) heat shock (85 degrees c for 10 min) and inoculation with lactobacillus plantarum aca-dc 287, (ii) heat shock and inoculation with l. plantarum aca-dc 146, (iii) heat shock and inoculation with lactobacillus pentosus isolated during previous studies ...200818680938
toxin gene pattern in bacillus cereus group strains isolated from sheep ricotta cheese. 200818685978
use of sensitivity analysis to aid interpretation of a probabilistic bacillus cereus spore lag time model applied to heat-treated chilled foods (repfeds).the microbiological safety and quality of refrigerated processed foods of extended durability (repfeds) relies on a combination of mild heat treatment and refrigeration, sometimes in combination with other inhibitory agents that are not effective when used alone. in this context, the output of a probabilistic model predicting the lag time of heat-treated bacillus cereus spores under realistic heat-treatment profile and chilled supply-chain conditions, has been investigated using a sensitivity an ...200818691785
antibacterial iridoid glucosides from eremostachys laciniata.eremostachys laciniata (l) bunge (family: lamiaceae alt. labiatae; subfamily: lamioideae) is one of the 15 endemic iranian herbs of the genus eremostachys. a decoction of the roots and flowers of e. laciniata has traditionally been taken orally for the treatment of allergies, headache and liver diseases. three antibacterial iridoid glucosides, phloyoside i (1), phlomiol (2) and pulchelloside i (3) have been isolated from the rhizomes of this plant. the structures of these compounds were elucidat ...200918693303
detection of quorum-sensing-related molecules in vibrio scophthalmi.cell-to-cell communication (also referred to as quorum sensing) based on n-acyl-homoserine lactones (ahls) is a widespread response to environmental change in gram-negative bacteria. ahls seem to be highly variable, both in terms of the acyl chain length and in the chemical structure of the radicals. another quorum sensing pathway, the autoinducer-2-based system, is present both in gram-positive and gram-negative bacteria. in this study the presence of signal molecules belonging to both quorum s ...200818700048
flavonoids from pterocaulon alopecuroides with antibacterial activity.three flavonoids, (2 r,3 r)-5,4'-dihydroxy-3'- o-methyl-7-(gamma,gamma-dimethylallyloxy)dihydroflavonol 1, (2 r,3 r)-5,7,4'-trihydroxy-3'- o-methyl-6-(alpha,alpha-dimethylallyl)dihydroflavonol 2, and (2 r,3 r)-5,7,4'-trihydroxy-6-(alpha,alpha-dimethylallyl)dihydroflavonol 3, together with three known flavonoids ( 4 - 6), were isolated from the aerial parts of pterocaulon alopecuroides. the structures of the compounds were determined by mass and by 1 d and 2 d nmr spectroscopy. screening of the a ...200818704884
bacillus cereus endocarditis in a permanent pacemaker: a case report.abstract:200818706120
structural and functional insights into o-methyltransferase from bacillus cereus.the specific substrates, mechanisms, and structures of the bacterial o-methyltransferases (omts) are not as well characterized as those of other omts. recent studies have suggested that bacterial omts catalyze regiospecific reactions that might be used to produce novel compounds. in this study, we investigated the structural and functional features of an omt from bacillus cereus (bcomt2). this enzyme catalyzes the o-methylation of flavonoids in vitro in an s-adenosylmethionine-dependent and regi ...200818706426
construction of a non toxic chimeric protein (l1-l2-b) of haemolysin bl from bacillus cereus and its application in hbl toxin detection.among the many potential virulence factors of b. cereus, haemolysin bl is a unique and potent three component pore forming toxin composed of a binding component, b, and two lytic components, l(1) and l(2). heterogeneity in nucleic acid and protein sequences of hbl components and problems during expression of l(1) and l(2) proteins in recombinant host due to their toxicity causes problems for development of specific detection systems based on pcr and immunoassay, respectively. commercially availa ...200818718851
[the regulation of sigmab activity and its role in some gram-positive food-borne pathogens--a review].the alternative sigma factor sigmab modulates the stress response of several gram-positive bacteria. not only does sigmab play a prominent role in sporulation in the gram-positive model organism bacillus subtilis, but it also contributes both directly and indirectly to bacterial virulence in the food-borne human pathogens bacillus cereus, listeria monocytogenes and staphylococcus aureus. as sigmab has been shown to regulate expression of both virulence and virulence-associated genes, it indicate ...200818720853
comparative survey of putrescine production from agmatine deamination in different bacteria.this article aims to study putrescine production in lactobacillus hilgardii strain x(1)b, an agmatine degrader isolated from wine, and to compare it with three other different species, previously reported as putrescine producers from agmatine: pseudomonas aeruginosa pao1, enterococcus faecalis atcc11700 and bacillus cereus cect 148(t). the effect of different biogenic amines, organic acids, cofactors, amino acids and sugars on putrescine production was evaluated. in some cases, a similar effect ...200818721677
critical factors for the performance of chip array-based electrical detection of dna for analysis of pathogenic bacteria.different factors influencing chip array-based electrical detection of dna for analysis of pathogenic bacteria were examined. both rehydration of capture probe layer of functionalized chip arrays and efficient hybridization of targets irrespective of their length resulted in signal enhancement when high-ionic phosphate-buffered saline (i.e., 600mm sodium chloride and 40mm disodium hydrogen phosphate) was used. similarly, placement of two adjacent capture and detection probe-binding sites at a te ...200818722993
from enzyme to zymogen: engineering vip2, an adp-ribosyltransferase from bacillus cereus, for conditional toxicity.the adenosine diphosphate (adp)-ribosyltransferase, vip2 (vegetative insecticidal protein), from bacillus cereus in combination with another protein from the same organism, vip1, has insecticidal activity against western corn rootworm larvae. the vip2 protein exerts its intracellular poisoning effect by modifying actin and preventing actin polymerization. due to the nature of this toxin, expression of vip2 in planta is lethal. in this work, we attempted to build an enzyme precursor (proenzyme, z ...200818723852
antimicrobial activity of extracts of edible wild and cultivated mushrooms against foodborne bacterial strains.the antimicrobial activity of aqueous, methanol, hexane, and ethyl acetate extracts from edible wild and cultivated mushrooms against nine foodborne pathogenic bacterial strains (escherichia coli o157:h7, salmonella enteritidis, shigella sonnei, vibrio parahaemolyticus, yersinia enterocolitica, bacillus cereus, clostridium perfringens, listeria monocytogenes, and staphylococcus aureus) was screened with a disk diffusion assay. twenty-nine of the 48 species tested had antimicrobial activity. meth ...200818724768
monitoring growth phase-related changes in phosphatidylcholine-specific phospholipase c production, adhesion properties and physiology of bacillus cereus vegetative cells.the physiological status and metabolic heterogeneity of bacillus cereus cells within a culture during an 8-h batch fermentation process was measured using flow cytometry (fcm). concurrently, production of the toxin, pc-plc, and the extent of cell adhesion of live and dead cells were monitored using novel fluorescent assays. flow cytometry analysis detected growth phase-related changes in the physiological profiles of cells over the course of the fermentation, with variation in the percentage of ...200818726128
fluorescence microscopy as a tool for in situ life detection.the identification of extant and, in some cases, extinct bacterial life is most convincingly and efficiently performed with modern high-resolution microscopy. epifluorescence microscopy of microbial autofluorescence or in conjunction with fluorescent dyes is among the most useful of these techniques. we explored fluorescent labeling and imaging of bacteria in rock and soil in the context of in situ life detection for planetary exploration. the goals were two-fold: to target non-earth-centric bio ...200818752456
effects of pulsed electric fields on pathogenic microorganisms of major concern in fluid foods: a review.pathogenic microorganisms such as escherichia coli o157:h7, salmonella spp., listeria monocytogenes, bacillus cereus, staphylococcus aureus, yersinia enterocolitica, and campylobacter jejuni have been implicated in foodborne diseases and outbreaks worldwide. these bacteria have been associated with the consumption of fresh fruit juices, milk, and dairy products, which are foodstuff, highly demanded by consumers in retails and supermarkets. nowadays, consumers require high quality, fresh-like, an ...200818756397
structural elucidation of the nonclassical secondary cell wall polysaccharide from bacillus cereus atcc 10987. comparison with the polysaccharides from bacillus anthracis and b. cereus type strain atcc 14579 reveals both unique and common structural features.nonclassical secondary cell wall polysaccharides constitute a major cell wall structure in the bacillus cereus group of bacteria. the structure of the secondary cell wall polysaccharide from bacillus cereus atcc 10987, a strain that is closely related to bacillus anthracis, was determined. this polysaccharide was released from the cell wall with aqueous hydrogen fluoride (hf) and purified by gel filtration chromatography. the purified polysaccharide, hf-ps, was characterized by glycosyl composit ...200818757856
transcriptional analysis of the conjugative plasmid paw63 from bacillus thuringiensis.the broad-host range plasmid paw63 is a model for the study of molecular mechanisms associated with conjugation in the gram-positive bacillus cereus group. its main features are a conjugative apparatus that includes type iv secretion system-like components and two group ii introns, b.th.i1 and b.th.i2, located within conjugation genes, as well as a putative regulatory control circuit. furthermore, paw63 shares a common backbone with pxo2, the second virulence plasmid of bacillus anthracis, and w ...200818761035
construction of bacillus thuringiensis wild-type s76 and cry- derivatives expressing a green fluorescent protein: two potential marker organisms to study bacteria-plant interactions.collectively, the species bacillus thuringiensis, bacillus cereus, and bacillus anthracis represent microorganisms of high economic, medical, and biodefense importance. although the genetic correlation and pathogenic characteristics have been extensively dissected, the ecological properties of these three species in their natural environments remain poorly understood. thus, a tractable marker for detecting these bacteria under specific environmental and physiological conditions is a valuable too ...200818772942
purification and some properties of an extracellular ribonuclease with antiviral activity against tobacco mosaic virus from bacillus cereus.a new ribonuclease (rnase) with tobacco mosaic virus inhibition was isolated and purified from bacillus cereus zh14 through ammonium sulfate precipitation, ultrafiltration, ion-exchange chromatography of deae-sephadex a-50 column, and gel chromatography of sephacryl s-200hr column. the enzyme was purified approximately 134-fold with a recovery of 9.2%. the rnase had an mw of 75.6 kda in sds-page, which differed from rnases reported previously. the inhibitory activity of the rnase in the purifica ...200918777010
[the effectiveness of glass bead sterilizer in the dental practice].although it is not fda-approved as inter-patients sterilization measure, in israel, glass bead sterilizer is still a common method for chair-side sterilization of small dental hand instruments, especially endodontic files. studies from the 1950-1970s achieved sterilization by the bead sterilizer within few seconds. nevertheless, there are no current evidence-based instructions for using this sterilizer. the study was designed to evaluate the effectiveness of this method in sterilization of endod ...200818780544
design and synthesis of 3-pyrazolyl-thiophene, thieno[2,3-d]pyrimidines as new bioactive and pharmacological activities.two series of 5-ethyl-2-amino-3-pyrazolyl-4-methylthiophenecarboxylate and 2-thioxo-n(3)-aminothieno[2,3-d]pyrimidines were prepared from 3,5-diethyl-2-amino-4-methylthio-phenecaboxylate and evaluated as anti-inflammatory, analgesic and ulcerogenic activities. among the compounds studied, compounds which containing the substituted hydrazide at c-3 position 7, 16, and 17a showed more potent anti-inflammatory and analgesic activities than the standard drug (indomethacin and aspirin), along without ...200818783947
investigating the effect of patulin, penicillic acid and edta on biofilm formation of isolates from dental unit water lines.this study investigated the effect of patulin and penicillic acid, two known quorum-sensing inhibitors, and the common biocide ethylenediaminetetraacetic acid (edta) on the biofilm formation and auto-inducer (ai)-2 production of three isolates from dental unit water lines, klebsiella sp., bacillus subtilis and bacillus cereus. penicillic acid on its own had no effect on the biofilm formation of all isolates, whereas in combination with edta, it enhanced biofilm formation significantly in klebsie ...200818787817
wound healing, anti-microbial and antioxidant potential of dendrophthoe falcata (l.f) ettingsh.dendrophthoe falcata (l.f) ettingsh (loranthaceae) of the order santalales, is used ethnomedicinally for treating ulcers, asthma, impotence, paralysis, skin diseases, and wounds. in this context, validations of the ethnotherapeutic claims of the plant in wound healing activity was studied, besides anti-microbial activity and antioxidant activity were performed to understand the mechanism of wound healing potency. the ethanolic extract of aerial parts of dendrophthoe falcata ethanolic extract (df ...200818790035
molecular identification of hemolymph-associated symbiotic bacteria in red imported fire ant larvae.the red imported fire ant (rifa) (solenopsis invicta buren), an exotic insect pest in texas, has become well established throughout the eastern part of the state. more aggressive than native ant species, rifa gradually have enlarged their range and spread north and west despite intense efforts to stop them. symbiotic bacteria have an important relationship in the midgut of fourth instar rifa larvae. however, the presence of symbiotic bacteria in hemolymph has not been explored. in this study, sy ...200818795363
genetic relationships between sympatric populations of bacillus cereus and bacillus thuringiensis, as revealed by rep-pcr genomic fingerprinting.the bacterial strain bacillus cereus is closely related to bacillus thuringiensis, although any genetic relationship between the two strains is still in debate. using rep-pcr genomic fingerprinting, we established the genetic relationships between brazilian sympatric populations of b. cereus and b. thuringiensis simultaneously collected from two geographically separate sites. we observed the formation of both b. thuringiensis and b. cereus clusters, as well as strains of b. cereus that are more ...200818797766
the efficacy of intravitreal vancomycin and dexamethasone in the treatment of experimental bacillus cereus endophthalmitis.bacillus cereus causes the most virulent and refractory form of endophthalmitis. the authors analyzed effectiveness of intravitreal vancomycin with or without dexamethasone for experimental bacillus cereus endophthalmitis.200818798079
enzymatic synthesis of isopropyl myristate using immobilized lipase from bacillus cereus mtcc 8372.a purified alkaline thermo-tolerant bacterial lipase from bacillus cereus mtcc 8372 was immobilized on a poly (mac-co-dma-cl-mbam) hydrogel. the hydrogel showed approximately 94% binding capacity for lipase. the immobilized lipase (2.36 iu) was used to achieve esterification ofmyristic acid and isopropanol in n-heptane at 65 degrees c under continuous shaking. the myristic acid and isopropanol when used at a concentration of 100 mm each in n-heptane resulted in formation of isopropyl myristate ( ...200818800597
antimicrobial effects of turkish propolis, pollen, and laurel on spoilage and pathogenic food-related microorganisms.the antimicrobial activities of propolis extract, pollen extract, and essential oil of laurel (laurus nobilis l.) at concentrations from 0.02% to 2.5% (vol/vol) were investigated on bacteria (bacillus cereus, bacillus subtilis, escherichia coli, salmonella typhimurium, staphylococcus aureus, yersinia enterocolitica, enterococcus faecalis, and listeria monocytogenes), yeasts (saccharomyces cerevisiae and candida rugosa), and molds (aspergillus niger and rhizopus oryzae). pollen has no antimicrobi ...200818800911
improving quantitative exposure assessment by considering genetic diversity of b. cereus in cooked, pasteurised and chilled foods.the natural contamination of foods with a bacterial pathogen frequently consists of a mixture of strains with their own characteristics of survival, growth potential and virulence. quantitative microbial risk assessment (qmra) must account for this genetic diversity to reflect the variability of the pathogen risk and to identify the genetic groups present at key stages of the food pathway. to describe the transmission dynamics of a heterogeneous population of b. cereus, we developed an exposure ...200818805600
efficacy of plant essential oils against foodborne pathogens and spoilage bacteria associated with ready-to-eat vegetables: antimicrobial and sensory screening.the objectives of this study were to evaluate the antimicrobial activity of plant essential oils (eos) against foodborne pathogens and key spoilage bacteria pertinent to ready-to-eat vegetables and to screen the selected eos for sensory acceptability. the eos basil, caraway, fennel, lemon balm, marjoram, nutmeg, oregano, parsley, rosemary, sage, and thyme were evaluated. the bacteria evaluated were listeria spp., staphylococcus aureus, lactobacillus spp., bacillus cereus, salmonella, enterobacte ...200818810868
antibacterial activity of xanthorrhizol isolated from curcuma xanthorrhiza roxb. against foodborne pathogens.xanthorrhizol, isolated from the ethanol extract of curcuma xanthorrhiza roxb., is a sesquiterpene compound with a molecular weight of 218. the aim of this study was to investigate the antibacterial activity of xanthorrhizol against foodborne pathogens. the antibacterial activity of xanthorrhizol was measured in terms of the mic and the mbc. mics and mbcs of xanthorrhizol against bacillus cereus, clostridium perfringens, listeria monocytogenes, staphylococcus aureus, salmonella typhimurium, and ...200818810881
biodegradation of chlorpyrifos in soil by enriched cultures.three aerobic bacterial consortia, ac, bc, and dc, developed from pesticide-contaminated soils of punjab were able to degrade chlorpyrifos after 21 days of incubation in basal medium by 54, 46, and 61% and chlorpyrifos (50 mg/l) in soil after 30 days by 50, 56, and 64%. pseudomonas aeruginosa, bacillus cereus, klebsiella sp., and serratia marscecens obtained from these consortia showed 84, 84, 81, and 80% degradation of chlorpyrifos (50 mg/l) in liquid medium after 20 days and 92, 60, 56, and 37 ...200918815830
digestion of rice straw and oil palm fronds by microflora from rumen and termite bacteria, in vitro.the digestion and volatile fatty acid (vfa) production from rice straw and oil palm fronds by cellulolytic bacteria isolated from the termite coptotermes curvignathus were investigated. the bacteria were acinetobacter strain raminalimon, enterobacter aerogenes strain razmin c, enterobacter cloacae strain razmin b, bacillus cereus strain razmin a and chryseobacterium kwangyangense strain cb. acinetobacter strain raminalimon is an aerobic bacterium, while the other species are facultative anaerobe ...200818817130
the bacillus subtilis ydjl (bdha) gene encodes acetoin reductase/2,3-butanediol dehydrogenase.bacillus subtilis is capable of producing 2,3-butanediol from acetoin by fermentation, but to date, the gene encoding the enzyme responsible, acetoin reductase/2,3-butanediol dehydrogenase (ar/bdh), has remained unknown. a search of the b. subtilis genome database with the amino acid sequences of functional ar/bdhs from saccharomyces cerevisiae and bacillus cereus resulted in the identification of a highly similar protein encoded by the b. subtilis ydjl gene. a knockout strain carrying a ydjl::c ...200818820069
[effects of low concentration heavy metals on biodegradation of bde209 by bacillus cereus].in view of joint contamination of heavy metals and polybrominated diphenyl ethers (pbdes) caused by electronic-wastes, analysis measures of gc-ms, icp, uv scanning, ftir, sem, etc. were used to research on the debromination of deca-brominated diphenyl ethers (bde209) and biodegradation capability under aerobic condition by combined bacillus cereus xpb and xpc, and the effects of low concentrations of heavy metals on the biodegradation of bde209 were also studied. the experimental results showed ...200818828385
circular dichroism and magnetic circular dichroism studies of the biferrous site of the class ib ribonucleotide reductase from bacillus cereus: comparison to the class ia enzymes.the rate limiting step in dna biosynthesis is the reduction of ribonucleotides to form the corresponding deoxyribonucleotides. this reaction is catalyzed by ribonucleotide reductases (rnrs) and is an attractive target against rapidly proliferating pathogens. class i rnrs are binuclear non-heme iron enzymes and can be further divided into subclasses. class ia is found in many organisms, including humans, while class ib has only been found in bacteria, notably some pathogens. both bacillus anthrac ...200818831534
[antibacterial activity of lactic acid bacteria community sfc-2 used for fermentation of air-dried crop straws].to evaluate the antibacterial activity of lactic acid bacteria community sfc-2 in fermented crop straw.200818837364
purification and characterization of protease and chitinase from bacillus cereus tku006 and conversion of marine wastes by these enzymes.a chitinase- and protease-producing bacterium was isolated and identified as bacillus cereus tku006. the better condition on our tests for protease and chitinase production was found when the culture was shaken at 25 degrees c for 2 days in 25 ml of medium containing 2% shrimp shell powder (w/v), 0.1% k(2)hpo(4), and 0.05% mgso(4).7h(2)o. the molecular masses of tku006 protease and chitinase determined by sodium dodecyl sulfate (sds)-polyacrylamide gel electrophoresis were approximately 39 and 3 ...200918843519
study on cytotoxicity and nucleotide sequences of enterotoxin fm of bacillus cereus isolated from various food sources.to determine the cytotoxicity of the crude culture broth containing enterotoxins of b. cereus and investigate the nucleotide sequences of ent fm among various isolates b. cereus.200818843874
crystal structure and enantiomer selection by d-alanyl carrier protein ligase dlta from bacillus cereus.ubiquitous d-alanylation of lipoteichoic acids modulates the surface charge and ligand binding of the gram-positive cell wall. disruption of the bacterial dltabcd gene involved in teichoic acid alanylation, as well as inhibition of the dlta protein, has been shown to increase a gram-positive bacterium's susceptibility to antibiotics. the dlta d-alanyl carrier protein ligase promotes a two-step process starting with adenylation of d-alanine. we have determined the 2.0 a resolution crystal structu ...200818847223
sphingomyelinase-induced domain shape relaxation driven by out-of-equilibrium changes of composition.sphingomyelinase (smase)-induced ceramide (cer)-enriched domains in a lipid monolayer are shown to result from an out-of-equilibrium situation. this is induced by a change of composition caused by the enzymatic production of cer in a sphingomyelin (sm) monolayer that leads to a fast sm/cer demixing into a liquid-condensed (lc), cer-enriched and a liquid-expanded, sm-enriched phases. the morphological evolution and kinetic dependence of cer-enriched domains is studied under continuous observation ...200918849413
identification of bacillus cereus group species associated with food poisoning outbreaks in british columbia, canada.food poisoning laboratories identify bacillus cereus using routine methods that may not differentiate all bacillus cereus group species. we recharacterized bacillus food-poisoning strains from 39 outbreaks and identified b. cereus in 23 outbreaks, b. thuringiensis in 4, b. mycoides in 1, and mixed strains of bacillus in 11 outbreaks.200818849447
identification of two mosquitocidal bacillus cereus strains showing different host ranges.mosquitocidal bacteria, m413 and c32 have been isolated from sediment samples collected from woodland and ditch, respectively. gas chromatographic analysis of fatty acids methyl esters (gc-fame) and 16s rrna gene sequence alignment results showed these isolates belong to bacillus cereus. the sds-page analysis of sporulated cultures of both isolates showed two major bands very similar in size. interestingly, however, m413 is mainly toxic to 4th instars of ochlerotatus taeniorhynchus whereas c32 i ...200918854193
the action of bacillus cereus and related species on the lecithin complex of egg yolk. 194818863606
film coating of seeds with bacillus cereus rs87 spores for early plant growth enhancement.the plant growth-promoting rhizobacterium bacillus cereus rs87 was previously reported to promote plant growth in various crops in both greenhouse and field trials. to apply as a plant growth promoting agent with practical use, it is essential to ease the burden of routine preparation of a fresh suspension of strain rs87 in laboratory. the objectives of this study were to investigate the feasibility of film-coating seeds with b. cereus rs87 spores for early plant growth enhancement and to reveal ...200818923555
the yvftu two-component system is involved in plcr expression in bacillus cereus.most extracellular virulence factors produced by bacillus cereus are regulated by the pleiotropic transcriptional activator plcr. among strains belonging to the b. cereus group, the plcr gene is always located in the vicinity of genes encoding the yvftu two-component system. the putative role of yvftu in the expression of the plcr regulon was therefore investigated.200818925929
genotypic and phenotypic diversity of bacillus spp. isolated from steel plant waste.molecular studies of bacillus diversity in various environments have been reported. however, there have been few investigations concerning bacillus in steel plant environments. in this study, genotypic and phenotypic diversity and phylogenetic relationships among 40 bacterial isolates recovered from steel plant waste were investigated using classical and molecular methods.200818928552
the effect of bacillus cereus on chicken erythrocytes. 194718935397
fulminant septicemia of bacillus cereus resistant to carbapenem in a patient with biphenotypic acute leukemia.we report a case of fulminant septicemia with bacillus cereus resistant to carbapenem. a 33-year-old man was suffering from febrile neutropenia (fn) on day 15 after the start of remission-induction therapy for biphenotypic acute leukemia under gut decontamination with polymyxin b and nystatin. meropenem, a carbapenem, was administered according to the guideline for fn. two days later (on day 17), he complained of severe abdominal pain, lost consciousness, went into sudden cardiopulmonary arrest, ...200818936889
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