Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| molecular analysis of human oral microbiota. | the application of molecular, mainly 16s ribosomal rna (rrna)-based approaches enables researchers to bypass the cultivation step and has proven its usefulness in studying the microbial composition in a variety of ecosystems, including the human oral cavity. in this mini-review, we describe the impact of these culture-independent approaches on our knowledge of the ecology of the human oral cavity and provide directions for future studies that should emphasize the role of specific strains, specie ... | 2005 | 15853975 |
| g(-) anaerobes-reactive cd4+ t-cells trigger rankl-mediated enhanced alveolar bone loss in diabetic nod mice. | diabetic patients experience a higher risk for severe periodontitis; however, the underlying mechanism remains unclear. we investigated the contribution of antibacterial t-cell-mediated immunity to enhanced alveolar bone loss during periodontal infection in nonobese diabetic (nod) mice by oral inoculation with actinobacillus actinomycetemcomitans, a g(-) anaerobe responsible for juvenile and severe periodontitis. the results show that 1) inoculation with a. actinomycetemcomitans in pre-diabetic ... | 2005 | 15855336 |
| inhibitory effects of areca nut extracts on phagocytosis of actinobacillus actinomycetemcomitans atcc 33384 by neutrophils. | areca quid chewers have a higher prevalence of periodontal disease than non-chewers. little is known about the influence of areca quid on the immune system. this study was to determine the possible effects of the areca nut on phagocytic activity of human neutrophils. | 2005 | 15857070 |
| implants in patients treated for generalized aggressive and chronic periodontitis: a 3-year prospective longitudinal study. | the aim of the present prospective longitudinal study of periodontally diseased and periodontally healthy patients was a clinical, microbiological, and radiographic comparison of teeth and implants and an assessment of the implant success rate. | 2005 | 15857093 |
| detection of actinobacillus actinomycetemcomitans but not bacteria of the red complex in aortic aneurysms by multiplex polymerase chain reaction. | aortic aneurysms affect an increasing number of elderly patients and cause considerable morbidity and mortality. the understanding of the mechanisms involved in the pathogenesis of aortic aneurysms is unclear and little is known about the role of microorganisms in the development of the condition. the aim of the present study was to examine aortic aneurysm samples for the presence of four putative periodontal pathogens: actinobacillus actinomycetemcomitans, treponema denticola, tannerella forsyt ... | 2005 | 15857100 |
| orthodontic treatment in a patient with papillon-lefèvre syndrome. | report of a combined periodontal and orthodontic treatment in a patient with papillon-lefevre syndrome (pls). | 2005 | 15857107 |
| luxs-based signaling affects streptococcus mutans biofilm formation. | streptococcus mutans is implicated as a major etiological agent in human dental caries, and one of the important virulence properties of this organism is its ability to form biofilms (dental plaque) on tooth surfaces. we examined the role of autoinducer-2 (ai-2) on s. mutans biofilm formation by constructing a gs-5 luxs-null mutant. biofilm formation by the luxs mutant in 0.5% sucrose defined medium was found to be markedly attenuated compared to the wild type. scanning electron microscopy also ... | 2005 | 15870324 |
| mechanism of internalization of the cytolethal distending toxin of actinobacillus actinomycetemcomitans. | cytolethal distending toxin (cdt), which is encoded by three genes, cdta, cdtb and cdtc, is now recognized to have a growing list of biological actions, including inhibition of cell cycle progression, promotion of apoptosis and stimulation of cytokine secretion. it appears that internalization of cdt is essential, at least for cell cycle blockade. using purified recombinant cdt proteins from the periodontopathic bacterium actinobacillus actinomycetemcomitans, the authors investigated which combi ... | 2005 | 15870449 |
| periodontitis as manifestation of crohn's disease in primary dentition: a case report. | the purpose of this case report is to detail an initial periodontal manifestation of crohn's disease in a 6-year-old boy. the first clinical diagnosis, localized aggressive periodontitis, was based on the microbiological isolation of actinobacillus actinomycetemcomitans from subgingival sites. on examination, gingival lesions, together with bleeding on probing, edema, and erythema, were observed. although an increased probing depth was detected, no radiographically visible alveolar bone loss was ... | 2004 | 15871451 |
| microbiological and clinical outcomes and patient satisfaction for two treatment options in the edentulous lower jaw after 10 years of function. | long-term data on microbiological and clinical outcome as well as on patient satisfaction after implant therapy in the edentulous mandible are limited. especially comparisons between fixed full prostheses (ffps) and overdentures (ods), or between anchoring systems for the latter are scarce. | 2005 | 15877747 |
| structural analysis of dispersin b, a biofilm-releasing glycoside hydrolase from the periodontopathogen actinobacillus actinomycetemcomitans. | bacteria in a biofilm are enmeshed in a self-synthesized extracellular polysaccharide matrix that holds the bacteria together in a mass and firmly attaches the bacterial mass to the underlying surface. a major component of the extracellular polysaccharide matrix in several phylogenetically diverse bacteria is pga, a linear polymer of n-acetylglucosamine residues in beta(1,6)-linkage. pga is produced by the gram-negative periodontopathogen actinobacillus actinomycetemcomitans as well as by the gr ... | 2005 | 15878175 |
| identification of actinobacillus actinomycetemcomitans using species-specific 16s rdna primers. | the purpose of this study was to develop species-specific pcr primers for use in the identification and detection of actinobacillus actinomycetemcomitans. these primers target variable regions of the 16s ribosomal rna coding gene (rdna). we assessed the specificity of the primers against 9 a. actinomycetemcomitans strains and 11 strains (3 species) of the haemophilus genus. primer sensitivity was determined by testing serial dilutions of the purified genomic dnas of a. actinomycetemcomitans atcc ... | 2005 | 15880099 |
| tobacco smoking and periodontal microflora in a saudi arabian population. | to explore the subgingival periodontal microflora in a saudi arabian population with a special focus on its relationship with various smoking habits. | 2005 | 15882210 |
| clinical and microbiological evaluation of the effectiveness of the nd:yap laser for the initial treatment of adult periodontitis. a randomized controlled study. | enhancement of the results obtained by scaling and planing is most often sought by using antimicrobial therapies. laser beams have been shown to be bactericidal and could possibly target pathogens more effectively and with fewer compliance problems than antiseptic solutions. | 2005 | 15882229 |
| susceptibilities of periodontopathogenic and cariogenic bacteria to antibacterial peptides, {beta}-defensins and ll37, produced by human epithelial cells. | antimicrobial peptides are one of the factors involved in innate immunity. the susceptibility of periodontopathogenic and cariogenic bacteria to the major antimicrobial peptides produced by epithelia was investigated. | 2005 | 15886266 |
| distinct transcriptional profiles characterize oral epithelium-microbiota interactions. | transcriptional profiling, bioinformatics, statistical and ontology tools were used to uncover and dissect genes and pathways of human gingival epithelial cells that are modulated upon interaction with the periodontal pathogens actinobacillus actinomycetemcomitans and porphyromonas gingivalis. consistent with their biological and clinical differences, the common core transcriptional response of epithelial cells to both organisms was very limited, and organism-specific responses predominated. a l ... | 2005 | 15888084 |
| identification of periodontal pathogens in atherosclerotic vessels. | epidemiological studies have shown that periodontitis may be associated with presence of atherosclerosis. dna from periodontal pathogens has been detected in atherosclerotic lesions, but viable oral bacteria have not yet been isolated from atherosclerotic plaques. the purpose of the present study was to determine if viable oral bacteria could be isolated from atherosclerotic lesions and if dna from periodontal pathogens could be detected by use of polymerase chain reaction (pcr) techniques. | 2005 | 15898933 |
| characteristics of periodontal microflora in acute myocardial infarction. | periodontitis has been linked to increased risk of cardiovascular diseases. systemic reactions associated with cardiovascular events may depend on characteristics of the subgingival microflora in periodontitis. our objectives were to compare the numbers of cultivable bacteria, composition of subgingival microflora and clonal distribution of actinobacillus actinomycetemcomitans (a. actinomycetemcomitans) in two groups of patients with generalized chronic periodontitis (gcp), one with an acute myo ... | 2005 | 15898935 |
| adjunctive antimicrobial therapy of periodontitis: long-term effects on disease progression and oral colonization. | this study assessed the long-term effects of adjunctive antimicrobial therapy on periodontal disease progression and oral colonization. | 2005 | 15898936 |
| debridement and local application of tetracycline in the management of persistent periodontitis. clinical and microbiological results after 12 months. | the purpose of this study was to evaluate the clinical and microbiological efficacy of local delivery of tetracycline in persistent periodontal lesions after topical therapy. | 2005 | 15902062 |
| clinical consequences of il-1 genotype on early implant failures in patients under periodontal maintenance. | implant failure and biologic complications such as periimplantitis are not completely avoidable. are there any genetic and microbiologic parameters that could be used to identify patients at risk for implant failure, preferably prior to treatment? this would result in improvement of the diagnostics, treatment decision, and risk assessment. | 2005 | 15903175 |
| gamma interferon positively modulates actinobacillus actinomycetemcomitans-specific rankl+ cd4+ th-cell-mediated alveolar bone destruction in vivo. | recent studies have shown the biological and clinical significance of signaling pathways of osteogenic cytokines rankl-rank/opg in controlling osteoclastogenesis associated with bone pathologies, including rheumatoid arthritis, osteoporosis, and other osteolytic disorders. in contrast to the inhibitory effect of gamma interferon (ifn-gamma) on rankl-mediated osteoclastogenesis reported recently, alternative new evidence is demonstrated via studies of experimental periodontitis using humanized no ... | 2005 | 15908374 |
| genetic basis for conversion of rough-to-smooth colony morphology in actinobacillus actinomycetemcomitans. | the basis of the rough-to-smooth conversion of actinobacillus actinomycetemcomitans was examined. smooth variants often contained mutations at the flp promoter region. replacing the mutated flp promoter with the wild-type promoter restored the rough phenotype. the expression level of the flp promoter was approximately 100-fold lower in smooth than in rough strains. mutations of the flp promoter are a cause of the rough-to-smooth conversion. | 2005 | 15908406 |
| genetic and functional analyses of the actinobacillus actinomycetemcomitans afeabcd siderophore-independent iron acquisition system. | the actinobacillus actinomycetemcomitans afeabcd iron transport system, the expression of which is controlled by iron and fur, was identified in three different isolates. the protein products of this locus are related to bacterial abc transporters involved in metal transport. transformation of the escherichia coli 1017 iron acquisition mutant with a plasmid harboring afeabcd promoted cell growth under iron-chelated conditions. however, insertion disruption of each of the afeabcd coding regions a ... | 2005 | 15908408 |
| periodontal pathogens: a quantitative comparison of anaerobic culture and real-time pcr. | periodontitis is a multi-factorial chronic inflammatory and destructive disease of the tooth-supporting tissues. quantitative anaerobic culture techniques have been used for microbial diagnosis of the different forms of the disease. the aim of this study was to compare real-time pcr with quantitative anaerobic culture for detection and quantification of 5 prominent periodontal pathogens. real-time pcr assays with the 16s rrna genes of actinobacillus actinomycetemcomitans, prevotella intermedia, ... | 2005 | 15919188 |
| [study on the detection of p. gingivalis, a. actinomycetemcomitans and t. denticola and the correlation between coinfections of the microbes and levels of chronic periodontitis lesion]. | to establish a 16s rdna multiplex polymerase chain reaction (pcr) for simultaneously detecting p. gingivalis, a. actinomycetemcomitans and t. denticola in clinical specimens of chronic periodontitis and to study the correlation between different modes of infection and severity of the disease. | 2005 | 15921614 |
| association between sharing of toothbrushes, eating and drinking habits and the presence of actinobacillus actinomycetemcomitans in moroccan adolescents. | actinobacillus actinomycetemcomitans is frequently detected in dental plaque collected from moroccan adolescents, and has been shown to be associated with clinical attachment loss in this population. the aim of this study was to assess whether behaviors such as the sharing of toothbrushes, and eating and drinking habits were associated with the presence of a. actinomycetemcomitans in moroccan adolescents. | 2005 | 15943761 |
| detection of periodontopathic bacteria and an oxidative stress marker in saliva from periodontitis patients. | we assessed the salivary levels of periodontopathic bacteria and 8-hydroxydeoxyguanosine (8-ohdg) in patients with periodontitis. the salivary levels of actinobacillus actinomycetemcomitans, porphyromonas gingivalis, and tannerella forsythia (formerly bacteroides forsythus) were assessed using real-time polymerase chain reaction. the 8-ohdg levels were determined using an enzyme-linked immunosorbent assay. the salivary levels of 8-ohdg, p. gingivalis, and t. forsythia in the periodontitis patien ... | 2005 | 15943765 |
| enhanced root planing and systemic metronidazole administration improve clinical and microbiological outcomes in a two-step treatment procedure. | recently we described a non-surgical two-step treatment concept, in which we distinguished between a first scaling and root planing step (srp) and an additional second enhanced root planing step (erp). until now it is difficult to determine how often a root surface should be instrumented during erp. | 2005 | 15948696 |
| the immunoreactivity of systemic antibodies to actinobacillus actinomycetemcomitans and porphyromonas gingivalis in adult periodontitis. | actinobacillus actinomycetemcomitans and porphyromonas gingivalis secrete several potent virulence factors and are known to be two of the major periodontal pathogens. in the present case-control study, the systemic immunoreactivity to a. actinomycetemcomitans exotoxins, cytolethal distending toxin (cdt) and leukotoxin was analyzed in adult subjects with periodontitis and in periodontally healthy controls. furthermore, systemic immunoreactivity to p. gingivalis was analyzed in these subjects. rea ... | 2005 | 15953243 |
| irsogladine maleate influences the response of gap junctional intercellular communication and il-8 of human gingival epithelial cells following periodontopathogenic bacterial challenge. | gingival epithelial cells first encounter periodontopathogenic bacteria and their metabolic products to produce inflammatory cytokines. gap junctional intercellular communication (gjic) is thought to play a critical role in cellular coordination in tissue homeostasis. gap junctions are structured by connexins (cxs). gjic response of gingival epithelial cells to the bacteria may be involved in the initiation of periodontal disease. irsogladine maleate (im) is known to enhance gjic through camp. i ... | 2005 | 15961066 |
| predictive value of clinical and microbiological parameters for the treatment outcome of scaling and root planing. | to compare the clinical and microbiological outcome of non-surgical periodontal therapy after 6 months with data obtained after hygienic phase or 6 weeks after completion of non-surgical therapy, in order to evaluate the value of clinical and microbiological parameters to predict treatment success. | 2005 | 15966873 |
| comparison of culture and real-time pcr for detection and quantification of five putative periodontopathogenic bacteria in subgingival plaque samples. | bacterial cultivation is a well-established method for analyzing plaque samples. real-time polymerase chain reaction (pcr) is a novel rapid method for the identification and quantification of periodontopathogenic bacteria that has been recently introduced. in this study, we compared real-time pcr with conventional anaerobic cultivation. | 2005 | 15966886 |
| detection of actinobacillus actinomycetemcomitans in unstimulated saliva of patients with chronic periodontitis. | the use of whole saliva has shown to be promising in detecting actinobacillus actinomycetemcomitans out of the subgingival environment. the objective of the present study was to evaluate the use of unstimulated saliva in detecting a. actinomycetemcomitans and to compare the subgingival and extracrevicular occurrence of this pathogen in brazilian subjects with chronic periodontitis. | 2005 | 15974843 |
| prevalence of periodontopathic bacteria in aggressive periodontitis patients in a chilean population. | actinobacillus actinomycetemcomitans is considered a major etiologic agent of aggressive periodontitis (agp). other periodontopathic bacteria such as porphyromonas gingivalis are also suspected of participating in aggressive periodontitis although the evidence to support this is controversial. the aim of the present study was to determine the prevalence of eight periodontopathic bacteria in chilean patients with agp. | 2005 | 15974855 |
| association between coinfection of porphyromonas gingivalis, actinobacillus actinomycetemcomitans and treponema denticola and periodontal tissue destruction in chronic periodontitis. | the association between the infection of porphyromonas gingivalis, actinobacillus actinomycetemcomitans and treponema denticola in chronic periodontitis (cp) and the severity of periodontal disease remains to be elucidated. the aim of this study was to investigate the subgingival infection frequencies of three periodontopathic bacteria in chinese cp patients and to evaluate the correlations between infection by these bacteria and periodontal destruction. | 2005 | 15978192 |
| periodontal disease and coronary heart disease: a reappraisal of the exposure. | results from studies relating periodontal disease to cardiovascular disease have been mixed. residual confounding by smoking and use of clinical measures of periodontal disease rather than measures of infection have been 2 major criticisms. the aims of this study were to investigate relationships between prevalent coronary heart disease (chd) and 2 exposures, (1) clinical periodontal disease and (2) igg antibodies to 17 oral organisms, and to evaluate the role of smoking in these relationships. | 2005 | 15983248 |
| prevalence of putative periodontopathogens in subgingival dental plaques from gingivitis lesions in korean orthodontic patients. | the objective of this study was to detect and compare the presence of periodontopathogens in the subgingival plaques of gingivitis lesions in adults who wore fixed orthodontic appliances, as opposed to adults who did not wear any orthodontic appliances. thirty-six individuals participated in this study. nineteen of these subjects did not wear any orthodontic appliances, and these subjects comprised the control group. the other 17 individuals had been wearing fixed orthodontic appliances for at l ... | 2005 | 15995644 |
| actinobacillus actinomycetemcomitans y4 capsular polysaccharide induces il-1beta mrna expression through the jnk pathway in differentiated thp-1 cells. | capsular polysaccharide from actinobacillus actinomycetemcomitans y4 (y4 cp) induces bone resorption in a mouse organ culture system and osteoclast formation in mouse bone marrow cultures, as reported in previous studies. we also found that y4 cp inhibits the release of interleukin (il)-6 and il-8 from human gingival fibroblast (hgf). thus y4 cp induces various responses in localized tissue and leads to the secretion of several cytokines. however, the effects of y4 cp on human monocytes/macropha ... | 2005 | 15996190 |
| an antibacterial surface on dental implants, based on the photocatalytic bactericidal effect. | it is well known that the moderately roughened surfaces of dental implants enhance direct bone-implant contact. however, rough implant surfaces, as compared to smooth surfaces, are thought to pose a higher risk of bacterial infection when exposed to the oral cavity. | 2005 | 15996357 |
| prevalence of periodontal pathogens in brazilians with aggressive or chronic periodontitis. | previous studies suggest differences between geographically and racially distinct populations in the prevalence of periodontopathic bacteria as well as greater periodontal destruction associated with infection by highly leucotoxic actinobacillus actinomycetemcomitans. the present study examined these hypotheses in brazilians with aggressive or chronic periodontitis. | 2005 | 15998269 |
| antimicrobial profiles of periodontal pathogens isolated from periodontitis patients in the netherlands and spain. | antimicrobial resistance of periodontal pathogens towards currently used antibiotics in periodontics has been investigated in a previous study. microbial resistance in the periodontal microflora was more frequently observed in spanish patients in comparison with dutch patients. the aim of the present study was to compare antimicrobial susceptibility profiles of five periodontal bacteria isolated from periodontitis patients in spain and in the netherlands. | 2005 | 15998275 |
| attachment of periodontal ligament cells onto various antibiotics-loaded gtr membranes. | this study compared the natures of various guided tissue regeneration (gtr) membranes impregnated with amoxicillin or tetracycline. whether the antibiotics-loaded membranes enhance the attachment of the periodontal ligament cells in the presence of oral pathogens was further analyzed. s mutans and a actinomycetemcomitans diminished the attachment of periodontal ligament cells onto the membranes as evaluated by sem analysis. these bacterial effects on cellular attachment could be reversed using t ... | 2005 | 16001739 |
| [methods of microbiological diagnosis in periodontal disease]. | microbiological findings together with clinical and radiological diagnosis are essential for rationale use of antibiotics in periodontal disease. methods used for microbiological diagnosis are: microscopy, cultivation, gas liquid chromatography, pcr, immuno-assays (elisa, immunofluorescence), fish. each of them has some advantages and disadvantages related to cost, accessibility, sensitivity, duration. the most used today are cultivation and pcr techniques. | 2004 | 16004241 |
| oral bacteria in the occluded arteries of patients with buerger disease. | recent studies have suggested that infectious organisms play a role in vascular diseases. in this study, to explore a possible link between oral infection and buerger disease, we investigated whether oral (periodontal) bacteria were present in occluded arteries removed from patients with characteristic buerger disease. | 2005 | 16012459 |
| genes of periodontopathogens expressed during human disease. | since many bacterial genes are environmentally regulated, the screening for virulence-associated factors using classical genetic and molecular biology approaches can be biased under laboratory growth conditions of a given pathogen, because the required conditions for expression of many virulence factors may not occur during in vitro growth. thus, technologies have been developed during the past several years to identify genes that are expressed during disease using animal models of human disease ... | 2002 | 16013215 |
| population studies of microbial ecology in periodontal health and disease. | it has been established that the bacterial diversity in any given environment is severely underestimated when assessed by means of culture-based techniques. yet, almost all currently available knowledge related to the periodontal microbiota in health and disease has been generated either by culture-based surveys or by methods that require prior species identification by culture. a handful of recent studies using culture-independent molecular methods providing 16s rrna sequences for both cultivab ... | 2002 | 16013217 |
| differential effect of the cytolethal distending toxin of actinobacillus actinomycetemcomitans on co-cultures of human oral cells. | the periodontal pathogen actinobacillus actinomycetemcomitans expresses a cytolethal distending toxin (cdt) that typically arrests the growth of eukaryotic cells at either the g0/g1 or g2/m phase of the cell cycle. it was previously found that cdt failed to arrest the growth of human periodontal ligament fibroblasts (hplfs) when grown in pure culture. in contrast, proliferation of an oral epithelial cell line was rapidly inhibited by the toxin. in this study, the feasibility of using mixed-cell ... | 2005 | 16014433 |
| resistance of human periodontal ligament fibroblasts to the cytolethal distending toxin of actinobacillus actinomycetemcomitans. | the cytolethal distending toxin (cdt) of actinobacillus actinomycetemcomitans is a typical member of this gram-negative bacterium holotoxin family that targets a wide spectrum of eukarytotic cells, typically causing cell cycle arrest at either the g(1) or g(2)/m phase of the cell cycle. in view of the possible role of the cdt as a prominent a. actinomycetemcomitans virulence factor in periodontal diseases, we have examined the effects of the toxin on primary cultures of human periodontal ligamen ... | 2005 | 16018764 |
| involvement of ganglioside gm3 in g(2)/m cell cycle arrest of human monocytic cells induced by actinobacillus actinomycetemcomitans cytolethal distending toxin. | actinobacillus actinomycetemcomitans produces a toxin called cytolethal distending toxin (cdt), which causes host cell dna damage leading to the induction of dna damage checkpoint pathways. cdt consists of three subunits, cdta, cdtb, and cdtc. cdtb is the active subunit of cdt and exerts its effect as a nuclease that damages nuclear dna, triggering cell cycle arrest. in the present study, we confirmed that the only combination of toxin proteins causing cell cycle arrest was that of all three rec ... | 2005 | 16040998 |
| [eikenella corrodens infective endocarditis]. | the hacek microorganisms (haemophilus spp, actinobacillus actinomycetemcomitans, cardiobacterium hominis, eikenella corrodens, and kingella kingae) account for 3% of the cases of endocarditis. they have the following similar clinical and microbiological properties: are gram-negative bacilli, more easily isolated in aerobic media; their cultures require prolonged incubation time for growing (mean, 3.3 days); and may be considered part of normal flora of upper respiratory tract and oropharynx. the ... | 2005 | 16041457 |
| [efficacy variation of erythromycin and spiramycin on periopathogens in aggressive periodontitis. an in vitro comparative study]. | erythromycin (ery) and spiramycin (spi) are the most frequently prescribed macrolides by dentists. however, the emergence of resistant anaerobic subgingival bacteria imposes an increased vigilance. this study aims to compare these macrolides efficacy on principal periopathogens. | 2005 | 16087299 |
| the effects of intra-amniotic injection of periodontopathic lipopolysaccharides in sheep. | periodontal disease may cause several complications of pregnancy, including fetal death. the purpose of this study was to investigate in sheep the effects of the intra-amniotic injection of lipopolysaccharide from 3 periodontopathic organisms and to compare these effects with those resulting from similar injection of escherichia coli lipopolysaccharide. the outcomes that were studied included the rates of fetal death and the features of inflammation and lung maturation in survivors. | 2005 | 16098849 |
| differential induction of human beta-defensin expression by periodontal commensals and pathogens in periodontal pocket epithelial cells. | to investigate the possible role of beta-defensins in gingival health and periodontal disease, we examined the effect of several stimuli on the expression of interleukin-8 (il-8), human beta-defensin-1, -2, -3, and -4 (hbd) in primary human diseased gingival epithelial (hge) cell cultures from periodontitis patients by quantitative taqman reverse transcription polymerase chain reaction (rt-pcr). | 2005 | 16101361 |
| influence of nicotine and cotinine on epithelial colonization by periodontopathogens. | since smoking is an established risk factor for the development of periodontitis, the present study investigated whether nicotine and cotinine can make epithelial cells more prone to colonization by periodontopathogens. | 2005 | 16101364 |
| influence of genetic background on transformation and expression of green fluorescent protein in actinobacillus actinomycetemcomitans. | the development of an electro-transformation system and the construction of shuttle plasmids for actinobacillus actinomycetemcomitans have enhanced the molecular analysis of virulence factors. however, inefficient transformation is frequently encountered. this study investigated the efficiency of electro-transformation and expression of green fluorescent protein (gfp) in 12 different a. actinomycetemcomitans strains. the influence of the plasmid vector, serotype, and phenotype were the major fac ... | 2005 | 16101962 |
| impact of smoking on the clinical, microbiological and immunological parameters of adult patients with periodontitis. | the aim of the current study was to assess the impact of smoking on the clinical indices, the humoral immune response and the detection frequency of putative periodontal pathogens in patients with periodontitis cross-sectionally and following therapy. | 2005 | 16104962 |
| the gingival crevicular fluid ciprofloxacin level in subjects with gingivitis and periodontitis, and its effects on clinical parameters. | the purpose of this study, conducted on patients with gingivitis and periodontitis, was twofold: to find out the serum and gingival crevicular fluid concentration of ciprofloxacin, which is a common drug used effectively against actinobacillus actinomycetemcomitans and to determine the effects of ciprofloxacin administration on clinical parameters. | 2005 | 16105092 |
| clinical characteristics of invasive haemophilus aphrophilus infections. | haemophilus aphrophilus, an oral fastidious gram-negative commensal with low pathogenicity, is a member of the hacek group (h. aphrophilus, h. paraphrophilus, actinobacillus actinomycetemcomitans, cardiobacterium hominis, eikenella corrodens, and kingella spp.), and a rare cause of human infections. we reviewed the characteristics of 8 cases of h. aphrophilus infections diagnosed in our hospital from 1990-2003, and an additional 20 cases identified from the medline database, from 1990 to 2003. t ... | 2005 | 16118675 |
| [localized aggressive periodontitis: a possible site-specific rather than tooth-specific disease?]. | the diagnosis of localized aggressive periodontitis includes first molar attachment loss as an obligatory criterion. this tooth-specific based diagnosis has never been questioned or tested previously. we present a rare case of aggressive periodontitis that developed during orthodontic treatment, which included extraction of right lower first molar and bodily movement of the second molar to the original first molar site. at the end of the orthodontic therapy, localized periodontal disease was dia ... | 2005 | 16121953 |
| systemic antibodies to the leukotoxin of the oral pathogen actinobacillus actinomycetemcomitans correlate negatively with stroke in women. | chronic infections and associated inflammatory markers are suggested risk factors for cardiovascular diseases (cvd) and stroke. the proinflammatory cytokine interleukin (il)-1beta is suggested to play a role in the regulation of local inflammatory responses in both cvd and periodontitis. the leukotoxin from the periodontal pathogen actinobacillus actinomycetemcomitans has recently been shown to cause abundant secretion of il-1beta from macrophages. the aim of the present study was to compare the ... | 2005 | 16123541 |
| transmission of periodontal bacteria and models of infection. | bacteria play an essential role in the aetiology of periodontitis. most bacterial species isolated from subgingival plaque are indigenous to the oral cavity. actinobacillus actinomycetemcomitans and porphyromonas gingivalis are detected infrequently in periodontal health, which makes these species prime candidates to study person-to-person transmission. the aim of the present study was to review the literature on transmission of these periodontal bacterial species. | 2005 | 16128826 |
| advances in the aetiology of periodontitis. group a consensus report of the 5th european workshop in periodontology. | 2005 | 16128829 | |
| analytical epidemiology of periodontitis. | to review the literature related to the analytical epidemiology of periodontitis generated over the past decade. this review does not deal with descriptive epidemiologic studies of the prevalence, extent and severity of periodontitis with respect to global geography, but focuses exclusively on analytical epidemiology issues, including the challenges posed by the use of different case definitions across studies, current theories and models of disease progression, and risk factors associated with ... | 2005 | 16128835 |
| microbiological diagnostics in periodontics: biological significance and clinical validity. | 2005 | 16135063 | |
| entry exclusion activity on conjugative plasmid pvt745. | conjugative plasmid transfer into a recipient cell containing the same or a closely related plasmid is inhibited by a mechanism called entry or surface exclusion. the function of entry exclusion is to reduce unproductive conjugation. the current study assessed the exclusion activity on conjugal plasmid pvt745 by conducting mating experiments with genetically distinguishable derivatives of this plasmid. our results demonstrate that a single gene, magb05, that is located in a gene cluster associat ... | 2006 | 16168481 |
| in vivo evaluation of the effect of essential oil-containing oral strips on salivary bacteria using the checkerboard method. | the aim of this study was to evaluate the antimicrobial effect of essential oil-containing oral strips on different species of the oral microbiota. | 2005 | 16170974 |
| real-time polymerase chain reaction for detection and quantification of bacteria in periodontal patients. | accurate laboratory tests for the detection and quantification of periodontopathogens in subgingival plaque samples of periodontal disease patients are becoming essential to study the pathogenesis of this polymicrobial condition. we used a real-time polymerase chain reaction (pcr) assay for the quantification of actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, dialister pneumosintes, campylobacter rectus, and micromonas micros as well as total eubacteria in ... | 2005 | 16171445 |
| tetraploid/diploid mosaicism with generalized aggressive periodontitis. | changes of chromosome number diploid to triploid or tetraploid states are rare in human pregnancies, where the main clinical features of tetraploidy are delayed growth and/or craniofacial abnormalities. the present report describes the oral features of tetraploid/diploid mosaicism. although the medical literature described the physical manifestations of this genetic abnormality, the oral features of this disorder were not previously described. | 2005 | 16171449 |
| microflora in oral ecosystems in subjects with hyposalivation due to medicines or of unknown origin. | to analyse the microbial flora in specific sites in 20 dentate (> or = 16 teeth) subjects with hyposalivation due to medicines or of unknown origin and in 20 controls matched according to age, sex and number of teeth. | 2005 | 16173383 |
| 16s rdna pcr-denaturing gradient gel electrophoresis in determining proportions of coexisting actinobacillus actinomycetemcomitans strains. | certain serotypes of actinobacillus actinomycetemcomitans seem to prefer coexistence in vivo. the 16s rdna pcr-denaturing gradient gel electrophoresis (dgge) was tested for its capability to distinguish coexisting a. actinomycetemcomitans strains of different serotypes or genetic lineages and to determine their proportions in vitro. the migration pattern of the pcr amplicon from serotype c differed from those of the other serotypes. contrary to the strains of serotypes c, d, and e, strains of se ... | 2006 | 16203051 |
| intragenomic recombination in the highly leukotoxic jp2 clone of actinobacillus actinomycetemcomitans. | the highly leukotoxic jp2 clone of actinobacillus actinomycetemcomitans is strongly associated with aggressive periodontitis in adolescents of african descent. dna fingerprinting using the frequently cutting restriction enzyme mspi and multilocus sequence typing (mlst) showed that five strains of this clone were genetically virtually identical, although ribotyping of the six rrn genes and ecori rflp analysis of the seven is150-like elements revealed differences. pcr analyses demonstrated that th ... | 2005 | 16207919 |
| caspase activation is involved in chronic periodontitis. | periodontitis, a common infectious disease, is initiated by various gram-negative bacteria and characterized by the destruction of the periodontal tissue. here, we investigated the role of caspases, intracellular proteases that are the key mediators of apoptosis. we show that activation of caspase-3 and caspase-7 is considerably enhanced in gingival tissue from patients with periodontitis. we also demonstrate in in vitro experiments that various periodontopathic bacteria exert a direct growth-su ... | 2005 | 16213496 |
| environmental influences on actinobacillus actinomycetemcomitans biofilm formation. | fresh clinical isolates of the periodontal pathogen actinobacillus actinomycetemcomitans have an adherent, rough colony morphology that transforms into a minimally adherent, smooth colony phenotype during successive in vitro passage. the objectives of this study were: (1) to compare biofilm formation of the rough (rvs) and smooth variants (svs) of several strains of a. actinomycetemcomitans grown under various environmental conditions and (2) to examine the dynamics of biofilm formation. a micro ... | 2006 | 16226214 |
| analysis of the presence of pathogens which predict the risk of disease at peri-implant sites through polymerase chain reaction (pcr). | the presence of dna of actinobacillus actinomycetemcomitans, porphyromonas gingivalis, and prevotella intermedia in the peri-implant sulcus samples of 19 partially edentulous patients was analyzed by polymerase chain reaction (pcr) and related to the depth of the peri-implant sulcus, bleeding on probing, and probable risk of disease. ten of those patients presented a history of periodontal disease and nine of those did not. the dna amplification of these pathogens was observed in seven samples, ... | 2005 | 16229357 |
| a searchable database for proteomes of oral microorganisms. | an online database of proteomes for two-dimensional electrophoresis (2de) gel data was constructed and it is now freely accessible through a web-based interface. proteins from three oral bacteria, streptococcus mutans ua159, actinobacillus actinomycetemcomitans hk1651, and porphyromonas gingivalis w83, whose genome databases are freely available, were separated by 2de, and protein spots were analyzed by matrix-assisted laser desorption/ionization time-of-flight (maldi-tof) and identified. about ... | 2005 | 16238593 |
| oral lactobacilli in chronic periodontitis and periodontal health: species composition and antimicrobial activity. | lactobacilli are known to play an important role in the maintenance of health by stimulating natural immunity and contributing to the balance of microflora. however, their role in chronic periodontitis is unclear. we aimed to identify oral lactobacilli in chronic periodontitis and periodontally healthy subjects, and to determine their antimicrobial activity against putative oral pathogens. | 2005 | 16238595 |
| cd14 and tlr4 gene polymorphisms in adult periodontitis. | bacterial deposits, smoking, and host genetic factors play a major role in an individual's predisposition to periodontitis. bacterial components are recognized by cd14 and toll-like receptor 4 (tlr4), resulting in a nf-kappab-based inflammatory response. we hypothesized that functional cd14 and tlr4 polymorphisms contribute to periodontitis susceptibility. we aimed to investigate the occurrence of cd14-260c>t, tlr4 299asp>gly, and 399thr>ile gene polymorphisms in adult periodontititis. dna was c ... | 2005 | 16246938 |
| involvement of porphyromonas gingivalis fima genotype in treatment outcome following non-surgical periodontal therapy. | porphyromonas gingivalis is considered a critical pathogen in periodontal diseases. it is classified into six genotypes based on diversity of the fima gene encoding fimbrillin. the present study evaluated the involvement of the fima genotype in treatment outcome following non-surgical periodontal therapy. | 2005 | 16253087 |
| serum antibodies to periodontal pathogens and markers of systemic inflammation. | we examined the relationship between serum antibodies against porphyromonas gingivalis and actinobacillus actinomycetemcomitans, and plasma fibrinogen and serum c-reactive protein (crp) in a nationally representative sample. | 2005 | 16268994 |
| clinical and microbiological characterization of periodontal abscesses. | the knowledge of clinical features, microbial composition and susceptibility to antimicrobials of periodontal abscesses has recently improved. this descriptive clinical and microbiological study provides more information on the characteristics of periodontal abscesses. | 2005 | 16268997 |
| distribution of 10 periodontal bacteria in saliva samples from japanese children and their mothers. | we analyzed the distribution of 10 periodontal bacteria species (porphyromonas gingivalis, tannerella forsythensis, prevotella intermedia, prevotella nigrescens, campylobacter rectus, eikenella corrodens, actinobacillus actinomycetemcomitans, capnocytophaga ochracea, capnocytophaga sputigena, and treponema denticola) in children, and then compared their distribution in those children and their mothers, with special attention given to three of the species known as the red complex (p. gingivalis, ... | 2006 | 16269128 |
| plasma levels of mannan-binding lectin in relation to periodontitis and smoking. | mannan-binding lectin (mbl) is an important molecule of innate immunity; it acts as an opsonin and stimulates the classical complement pathway. moreover, it has been suggested that mbl acts as a weak acute phase protein. we investigated whether mbl levels are increased in periodontitis, and we tested whether individuals deficient for mbl are more susceptible to periodontitis. | 2005 | 16274307 |
| immune response: the key to bone resorption in periodontal disease. | periodontal disease infection with oral biofilm microorganisms initiates host immune response and signs of periodontitis, including bone resorption. this review delineates some mechanisms underlying the host immune response in periodontal infection and alveolar bone resorption. activated t lymphocytes have been historically implicated in experimental periodontal bone resorption. an experimental rat adoptive transfer/gingival challenge periodontal disease model has been demonstrated to require an ... | 2005 | 16277573 |
| identification of a genomic island of actinobacillus actinomycetemcomitans. | horizontal gene transfer (hgt) is a process by which bacteria acquire genes from organisms of distant taxa. hgt is now recognized as a major driving force in the evolution of bacterial pathogens. through this process, bacteria may accumulate blocks of dna such as genomic islands (geis) that encode fitness or virulence factors. the periodontal pathogen a. actinomycetemcomitans has been known to exhibit variable virulence potential. it is postulated that geis may play a role in modifying the virul ... | 2005 | 16277576 |
| inhibitory effects of a super pulsed carbon dioxide laser at low energy density on periodontopathic bacteria and lipopolysaccharide in vitro. | previous studies have described the effect of irradiation by a carbon dioxide (co2) laser at high energy density on oral bacteria, and various side-effects have also been observed. however, no published studies have examined the effect of irradiation by a co2 laser at low energy density on oral bacteria. the purpose of this study was to investigate the effects of super pulsed co2 laser irradiation on periodontopathic bacteria and lipopolysaccharide (lps). | 2005 | 16302925 |
| streptococci dominate the diverse flora within buccal cells. | previously, we reported that intracellular actinobacillus actinomycetemcomitans, porphyromonas gingivalis, and tannerella forsythensis were present within buccal epithelial cells from human subjects, as lesser components of a polymicrobial flora. in this study, we further characterized that intracellular flora by using the same double-labeling techniques to identify fusobacterium nucleatum, prevotella intermedia, oral campylobacter species, eikenella corrodens, treponema denticola, gemella haemo ... | 2005 | 16304448 |
| characterization of point mutations in the cdta gene of the cytolethal distending toxin of actinobacillus actinomycetemcomitans. | the cdt is a family of gram-negative bacterial toxins that typically arrest eukaryotic cells in the g0/g1 or g2/m phase of the cell cycle. the toxin is a heterotrimer composed of the cdta, cdtb and cdtc gene products. although it has been shown that the cdta protein subunit binds to cells in culture and in an enzyme-linked immunosorbent assay (celisa) the precise mechanisms by which cdta interacts with cdtb and cdtc has not yet been clarified. in this study we employed a random mutagenesis strat ... | 2005 | 16313618 |
| a clinical study: melaleuca, manuka, calendula and green tea mouth rinse. | a novel mouthrinse (ind 61,164) containing essential oils and extracts from four plant species (melaleuca alternifolia, leptospermum scoparium, calendula officinalis and camellia sinensis) were tested. this study aimed to evaluate the safety, palatability and preliminary efficacy of the rinse. fifteen subjects completed the phase i safety study. seventeen subjects completed the phase ii randomized placebo-controlled study. plaque was collected, gingival and plaque indices were recorded (baseline ... | 2005 | 16317652 |
| actinobacillus actinomycetemcomitans lipopolysaccharide stimulates the phosphorylation of p44 and p42 map kinases through cd14 and tlr-4 receptor activation in human gingival fibroblasts. | tyrosine phosphorylation is an early step in lipopolysaccharide (lps) stimulated monocytes and macrophages that appears to play a key role in signal transduction. we have demonstrated that lps purified from actinobacillus actinomycetemcomitans also increases protein tyrosine phosphorylation in human gingival fibroblasts (hgf). this effect was elicited rapidly after lps stimulation at concentrations that stimulate anti-bacterial responses in human gingival fibroblasts. two main proteins, with an ... | 2006 | 16318859 |
| proceedings of the 8th european oral microbiology workshop, halkidiki, greece, may 12-15, 2005. abstracts. | 2005 | 16326934 | |
| [epidemiological study of the prevalence periodontal pathogeneus microflorae of oral cavity in the population of russia]. | 2005 | 16329206 | |
| prospective study of the long term change of the oral flora after radiation therapy. | the aim of this prospective study was to evaluate the long term change in oral pathogens following radiation therapy. | 2006 | 16341728 |
| [experience in medical ozone use for root canal treatment]. | the results of clinical and laboratory assessment of effectiveness of psr-diagnosis are presented. the high efficacy of ozone therapy is revealed. the findings demonstrate the reduction of number in the micro-organisms in root canal: actinobacillus actinomycetemcomitans from 31.25% to 10.21%; bacteriodes forsythus from 68.75% to 15.50%; treponema denticola from 37.5% to 11.4%; porphyromonas gingivalis from 56.25% to 45%; prevotella intermedia from 16% to 0%. | 2005 | 16353031 |
| the use of diode lasers in periodontal therapy: literature review and suggested technique. | 2005 | 16358809 | |
| bacterial-responsive b lymphocytes induce periodontal bone resorption. | host immune responses play a key role in periodontal diseases. we have found that b lymphocytes in human periodontal lesions bear abundant receptor activator of nf-kappab ligand (rankl), a major factor in the regulation of osteoclast differentiation. the purpose of this study was to evaluate actinobacillus actinomycetemcomitans-responsive b lymphocytes in their level of rankl expression and their effects on periodontal bone resorption. congenitally athymic rowett rats received injections of form ... | 2006 | 16365458 |
| immediate effect of instrumentation on the subgingival microflora in deep inflamed pockets under strict plaque control. | to investigate (1) reduction in the number of microorganisms obtained directly after subgingival instrumentation, (2) rate of bacterial re-colonization during 2 weeks, under supragingival plaque-free conditions. | 2006 | 16367855 |
| resistance of fluorescent-labelled actinobacillus actinomycetemcomitans strains to phagocytosis and killing by human neutrophils. | neutrophils are initially the predominant cells involved in the host defence of bacterial infections, including periodontal disease. aggressive periodontitis is associated with actinobacillus actinomycetemcomitans, a gram-negative capnophilic microorganism. infections caused by a. actinomycetemcomitans are not resolved by the host immune response despite the accumulation of neutrophils at the site of inflammation. to better understand the role of natural host defence mechanisms in a. actinomycet ... | 2006 | 16367867 |
| serum antibodies to oral anaerobic bacteria in patients with rheumatoid arthritis. | this study was conducted to determine the component that causes the disease in rheumatoid arthritis (ra), which shows great resemblance to periodontitis in a pathologic context. | 2005 | 16369381 |
| characterization of immunoaffinity purified peptidoglycan-associated lipoprotein of actinobacillus actinomycetemcomitans. | peptidoglycan-associated lipoprotein (pal) is a highly conserved structural outer membrane protein among gram-negative bacteria. in some species, it is proinflammatory and released extracellularly. we purified a newly identified pal (aapal) of a periodontal pathogen actinobacillus actinomycetemcomitans by using aapal antipeptide antibodies coupled to immunoaffinity chromatography column. no protein impurities originating in a. actinomycetemcomitans were found in the final product. sera from pati ... | 2006 | 16378764 |