Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| design of potent fluoro-substituted chalcones as antimicrobial agents. | owing to ever-increasing bacterial and fungal drug resistance, we attempted to develop novel antitubercular and antimicrobial agents. for this purpose, we developed some new fluorine-substituted chalcone analogs (3, 4, 9-15, and 20-23) using a structure-activity relationship approach. target compounds were evaluated for their antitubercular efficacy against mycobacterium tuberculosis h37rv and antimicrobial activity against five common pathogenic bacterial and three common fungal strains. three ... | 2017 | 28118738 |
| distinguishing potential bacteria-tumor associations from contamination in a secondary data analysis of public cancer genome sequence data. | a variety of bacteria are known to influence carcinogenesis. therefore, we sought to investigate if publicly available whole genome and whole transcriptome sequencing data generated by large public cancer genome efforts, like the cancer genome atlas (tcga), could be used to identify bacteria associated with cancer. the burrows-wheeler aligner (bwa) was used to align a subset of illumina paired-end sequencing data from tcga to the human reference genome and all complete bacterial genomes in the r ... | 2017 | 28118849 |
| depupylase dop requires inorganic phosphate in the active site for catalysis. | analogous to eukaryotic ubiquitination, proteins in actinobacteria can be post-translationally modified in a process referred to as pupylation, the covalent attachment of prokaryotic ubiquitin-like protein pup to lysine side chains of the target protein via an isopeptide bond. as in eukaryotes, an opposing activity counteracts the modification by specific cleavage of the isopeptide bond formed with pup. however, the enzymes involved in pupylation and depupylation have evolved independently of ub ... | 2017 | 28119453 |
| ppe surface proteins are required for heme utilization by mycobacterium tuberculosis. | iron is essential for replication of mycobacterium tuberculosis, but iron is efficiently sequestered in the human host during infection. heme constitutes the largest iron reservoir in the human body and is utilized by many bacterial pathogens as an iron source. while heme acquisition is well studied in other bacterial pathogens, little is known in m. tuberculosis to identify proteins involved in heme utilization by m. tuberculosis, a transposon mutant library was screened for resistance to the t ... | 2017 | 28119467 |
| mycobacterial esx-1 secretion system mediates host cell lysis through bacterium contact-dependent gross membrane disruptions. | mycobacterium tuberculosis and mycobacterium marinum are thought to exert virulence, in part, through their ability to lyse host cell membranes. the type vii secretion system esx-1 [6-kda early secretory antigenic target (esat-6) secretion system 1] is required for both virulence and host cell membrane lysis. both activities are attributed to the pore-forming activity of the esx-1-secreted substrate esat-6 because multiple studies have reported that recombinant esat-6 lyses eukaryotic membranes. ... | 2017 | 28119503 |
| duration of pulmonary tuberculosis infectiousness under adequate therapy, as assessed using induced sputum samples. | a sputum culture is the most reliable indicator of the infectiousness of pulmonary tuberculosis (ptb); however, a spontaneous sputum specimen may not be suitable. the aim of this study was to evaluate the infectious period in patients with non-drug-resistant (dr) ptb receiving adequate standard chemotherapy, using induced sputum (is) specimens. | 2017 | 28119744 |
| health-related quality of life, depression and anxiety in hospitalized patients with tuberculosis. | much of the attention of tuberculosis (tb) programs is focused on outcomes of microbiological cure and mortality, and health related quality of life (hrql) is undervalued. also, tb patients have a significantly higher risk of developing depression and anxiety compared with those in the general population. we intend to evaluate the hrql and the prevalence of symptoms of depression and anxiety in hospitalized patients with tb. | 2017 | 28119749 |
| mycobacterium tuberculosis esat6 and cpf10 induce adenosine deaminase 2 mrna expression in monocyte-derived macrophages. | delayed hypersensitivity plays a large role in the pathogenesis of tuberculous pleural effusion (tpe). macrophages infected with live mycobacterium tuberculosis (mtb) increase the levels of adenosine deaminase2 (ada2) in the pleural fluid of tpe patients. however, it is as yet unclear whether ada2 can be produced by macrophages when challenged with mtb antigens alone. this study therefore evaluated the levels of ada2 mrna expression, using monocyte-derived macrophages (mdms) stimulated with mtb ... | 2017 | 28119750 |
| genetic structure and drug susceptibility patterns of mycobacterium tuberculosis complex strains responsible of human pulmonary tuberculosis in the major rearing region in cameroon. | background. cameroon this last decade continues to present a low contribution of m. africanum and m. bovis in human tuberculosis (tb), while m. bovis was prevalent in cattle but all these pieces of information only concerned west and center regions. methods. we carried out the first study in adamaoua, one of the most rearing regions of cameroon, on the genetic structure and drug susceptibility of the mtbc strains isolated from newly diagnosed sputum smear-positive patients aged 15 years and abov ... | 2016 | 28119925 |
| clinical-pathological conference series from the medical university of graz : case no 162: a 30-year-old woman from nigeria with fever 3 months postpartum. | 2017 | 28120106 | |
| the role of universal stress proteins in bacterial infections. | universal stress proteins are ubiquitously expressed in bacteria, archaea and plants and other eukaryotes. a general property of usps is their role in adaptation of bacteria to oxidative stress, high temperature, low ph and/or hypoxia. there is increasing evidence that these proteins facilitate the adaption of bacterial pathogens to the human host environment, thereby facilitating colonisation and pathogenicity. usps in mycobacterium tuberculosis are well studied and may play a role in latency o ... | 2017 | 28120710 |
| [au]/[ag]-catalysed expedient synthesis of branched heneicosafuranosyl arabinogalactan motif of mycobacterium tuberculosis cell wall. | emergence of multidrug-resistant and extreme-drug-resistant strains of mycobacterium tuberculosis (mtb) can cause serious socioeconomic burdens. arabinogalactan present on the cellular envelope of mtb is unique and is required for its survival; access to arabinogalactan is essential for understanding the biosynthetic machinery that assembles it. isolation from nature is a herculean task and, as a result, chemical synthesis is the most sought after technique. here we report a convergent synthesis ... | 2017 | 28120821 |
| mycofactocin-associated mycobacterial dehydrogenases with non-exchangeable nad cofactors. | during human infection, mycobacterium tuberculosis (mtb) survives the normally bacteriocidal phagosome of macrophages. mtb and related species may be able to combat this harsh acidic environment which contains reactive oxygen species due to the mycobacterial genomes encoding a large number of dehydrogenases. typically, dehydrogenase cofactor binding sites are open to solvent, which allows nad/nadh exchange to support multiple turnover. interestingly, mycobacterial short chain dehydrogenases/redu ... | 2017 | 28120876 |
| evaluation of the impact of polyclonal infection and heteroresistance on treatment of tuberculosis patients. | mixed strain infections of mycobacterium tuberculosis make diagnosis, treatment, and control of tuberculosis (tb) more difficult. this study was aimed to evaluate the relationship between mixed infections, antibiotic resistance patterns and treatment of tb patients. in this study, among 2850 suspected tb clinical samples, a total of ninety-six clinical samples from 66 tb confirmed patients were subjected to the 24-locus variable-number tandem repeat method to evaluate the prevalence of mixed inf ... | 2017 | 28120910 |
| assessment of metabolic changes in mycobacterium smegmatis wild-type and alr mutant strains: evidence of a new pathway of d-alanine biosynthesis. | in mycobacteria, d-alanine is an essential precursor for peptidoglycan biosynthesis. the only confirmed enzymatic pathway to form d-alanine is through the racemization of l-alanine by alanine racemase (alr, ec 5.1.1.1). nevertheless, the essentiality of alr in mycobacterium tuberculosis and mycobacterium smegmatis for cell survivability in the absence of d-alanine has been a point of controversy with contradictory results reported in the literature. to address this issue, we examined the effects ... | 2017 | 28121156 |
| prospective etiological investigation of community-acquired pulmonary infections in hospitalized people living with hiv. | the study of the etiological agents of community-acquired pulmonary infections is important to guide empirical therapy, requires constant updating, and has a substantial impact on the prognosis of patients. the objective of this study is to determine prospectively the etiology of community-acquired pulmonary infections in hospitalized adults living with hiv. patients were submitted to an extended microbiological investigation that included molecular methods. the microbiological findings were eva ... | 2017 | 28121925 |
| targeting proteasomes in infectious organisms to combat disease. | proteasomes are multisubunit, energy-dependent, proteolytic complexes that play an essential role in intracellular protein turnover. they are present in eukaryotes, archaea, and in some actinobacteria species. inhibition of proteasome activity has emerged as a powerful strategy for anticancer therapy and three drugs have been approved for treatment of multiple myeloma. these compounds react covalently with a threonine residue located in the active site of a proteasome subunit to block protein de ... | 2017 | 28122162 |
| mtb-specific lymphocyte responses are impaired in tuberculosis patients with pulmonary cavities. | tuberculosis (tb), an infectious disease caused by the bacillus mycobacterium tuberculosis (mtb), is a global health problem. because the failing immune response in the lung can lead to formation of a pulmonary cavity, this study was designed to clarify mtb-specific lymphocyte responses in tb patients with pulmonary cavities. | 2017 | 28122644 |
| mycobacterium tuberculosis membrane vesicles inhibit t cell activation. | mycobacterium tuberculosis utilizes multiple mechanisms to evade host immune responses, and inhibition of effector cd4(+) t cell responses by m. tuberculosis may contribute to immune evasion. tcr signaling is inhibited by m. tuberculosis cell envelope lipoglycans, such as lipoarabinomannan and lipomannan, but a mechanism for lipoglycans to traffic from m. tuberculosis within infected macrophages to reach t cells is unknown. in these studies, we found that membrane vesicles produced by m. tubercu ... | 2017 | 28122965 |
| ag85b synergizes with esat-6 to induce efficient and long-term immunity of c57bl/6 mice primed with recombinant bacille calmette-guerin. | the latest probable scenario in vaccination strategies is to prime one live attenuated vaccine candidate followed by boost dose of second vaccine candidate. in the present study, we primed the mice with a recombinant bacille calmette-guerin (bcg) comprising ag85b and esat-6 followed by boost doses of ag85b, esat-6 and ag85b-esat-6 fusion protein in the dda adjuvant, separately. after boost doses of 8 and 12 weeks, the levels of antigen-stimulated t cells secreting interferon (ifn)-γ, the content ... | 2017 | 28123491 |
| synthesis of adamantane aminoethers with antitubercular potential. | intrigued by the fact that aminoadamantane derivatives, bearing the active 1,2-ethylenediamine moiety, are promising antitubercular agents, we report herein on the synthesis and the antitubercular evaluation of n,n'-substituded-4,4'-[adamantane-2,2-diyl]bis(phenoxyalkyl amines) 1a-g, 1,1'-{4,4'-[adamantane-1,3-diyl]bis(phenoxyalkyl)} amines 2a-f, n,n'-substituded-[4-(1-adamantane)phenoxy] alkylamines) 3a-d and 2-[4-(dimethylaminoalkoxy)phenyl]adamantan-2-ol 4a,b, methods: a substituted diarylmet ... | 2017 | 28124614 |
| toxoplasma gondii gra7-targeted asc and pld1 promote antibacterial host defense via pkcα. | tuberculosis is a global health problem and at least one-third of the world's population is infected with mycobacterium tuberculosis (mtb). mtb is a successful pathogen that enhances its own intracellular survival by inhibiting inflammation and arresting phago-lysosomal fusion. we previously demonstrated that toxoplasma gondii (t. gondii) dense granule antigen (gra) 7 interacts with tnf receptor-associated factor 6 via myeloid differentiation primary response gene 88, enabling innate immune resp ... | 2017 | 28125719 |
| ruptured aortic aneurysm due to mycobacterium bovis bcg with a delayed bacteriological diagnosis due to false negative result of the mpb 64 immunochromatographic assay. | adjuvant therapy with bacillus calmette-guerin (bcg), a live attenuated strain of mycobacterium bovis, has become the treatment of choice for low-risk superficial bladder carcinoma following transurethral resection of the bladder. complications following vesical bcg instillations are uncommon but, in some cases, severe side-effects can occur such as sepsis or mycotic aneurysm. besides usual laboratory techniques used for the diagnosis of mycobacterium tuberculosis complex (mtbc) infections (smea ... | 2017 | 28126017 |
| rv1458c: a new diagnostic marker for identification of mycobacterium tuberculosis complex in a novel duplex pcr assay. | we explored the efficiency of rv1458c, the gene encoding a putative abc drug transporter specific for the mycobacterium tuberculosis complex (mtbc), as a diagnostic marker. | 2017 | 28126044 |
| rapid screening for mycobacterium tuberculosis complex in clinical elephant trunk wash samples. | mycobacterium tuberculosis can infect and be transmitted between elephants and humans. in elephants, the 'gold standard' reference test for detection of tuberculosis is culture, which takes a minimum of eight weeks for results and has limited sensitivity. a screening test that is rapid, easily implemented, and accurate is needed to aid in diagnosis of tuberculosis in elephants. ninety-nine clinical trunk wash samples obtained from 33 elephants were utilized to validate three molecular extraction ... | 2017 | 28126601 |
| the -938c>a polymorphism in myd88 is associated with susceptibility to tuberculosis: a pilot study. | introduction. tuberculosis (tb) is a major disease worldwide, caused by mycobacterium tuberculosis (mtb) infection. the toll-like receptor (tlr) pathway plays a crucial role in the recognition of mtb. aim. the present study aimed to investigate the involvement of myeloid differentiation primary response protein 88 (myd88) gene polymorphisms in tb. materials and methods. a total of 103 tb cases and 92 control subjects were genotyped for the myd88 -938c>a (rs4988453) and 1944c>g (rs4988457) polymo ... | 2016 | 28127112 |
| high-throughput automated microfluidic sample preparation for accurate microbial genomics. | low-cost shotgun dna sequencing is transforming the microbial sciences. sequencing instruments are so effective that sample preparation is now the key limiting factor. here, we introduce a microfluidic sample preparation platform that integrates the key steps in cells to sequence library sample preparation for up to 96 samples and reduces dna input requirements 100-fold while maintaining or improving data quality. the general-purpose microarchitecture we demonstrate supports workflows with arbit ... | 2017 | 28128213 |
| m2 macrophages or il-33 treatment attenuate ongoing mycobacterium tuberculosis infection. | the protective effects of mycobacterial infections on lung allergy are well documented. however, the inverse relationship between tuberculosis and type 2 immunity is still elusive. although type 1 immunity is essential to protection against mycobacterium tuberculosis it might be also detrimental to the host due to the induction of extensive tissue damage. here, we determined whether lung type 2 immunity induced by allergen sensitization and challenge could affect the outcome of m. tuberculosis i ... | 2017 | 28128217 |
| matrix metalloproteinase-1 promoter -1607 bp 1g/2g polymorphism associated with increased risk of spinal tuberculosis in southern chinese han population. | spinal tuberculosis is the most common form of musculoskeletal tuberculosis. the expression of matrix metalloproteinase-1 (mmp-1) is increased in cells with mycobacterium tuberculosis infection. mmp-1 plays a curial role in extracellular matrix degradation during the progression of tuberculosis. although the 1g/2g polymorphism in mmp-1-1607 influences its transcription, its role in spinal tuberculosis remains unknown. | 2017 | 28129430 |
| bias in detection of mycobacterium tuberculosis polyclonal infection: use clinical samples or cultures? | the application of miru-vntr has unveiled that infection by mycobacterium tuberculosis can be polyclonal. our comparative study demonstrated that based on the studied samples (clinical specimen or culture) detection of polyclonal m. tuberculosis infection can be significantly different. | 2017 | 28130122 |
| diverse effects of mycobacterial proline-proline-glutamic acid proteins upon interaction with host macrophages. | the proline-proline-glutamic acid (ppe) family proteins are abundant only in pathogenic mycobacteria, but their general functions are far from unveiled. to investigate their roles in how mycobacterium tuberculosis (mtb) resists killing by the host, 25 ppe recombinant mycobacterium smegmatis strains that overexpress mtb ppe proteins were constructed. during phagocytosis, a similar amount of intracellular bacteria was observed at 2 h post-infection (hpi) for 24 ppe recombinants, while a 50% reduct ... | 2017 | 28130364 |
| analysis of uracil phosphoribosyltransferase expression in mycobacterium tuberculosis and evaluation of upp knockout strain in infected mice. | the upp (rv3309c)-encoded uracil phosphoribosyltransferase from mycobacterium tuberculosis (mtuprt) converts uracil and 5-phosphoribosyl-α-1-pyrophosphate into pyrophosphate and uridine 5΄-monophosphate, the precursor of all pyrimidine nucleotides. a m. tuberculosis knockout strain for upp gene was generated by allelic replacement. knockout and complemented strains were validated by a functional assay of uracil incorporation. a basal level of mtuprt expression is shown to be independent of eithe ... | 2017 | 28130366 |
| endogenous endophthalmitis due to roseomonas mucosa presenting as a subretinal abscess. | endogenous bacterial endophthalmitis is an infrequently reported entity. although roseomonas mucosa has been reported to cause systemic infections in immunosuppressed individuals, ocular infection due to roseomonas has been rarely reported in literature previously. | 2017 | 28130734 |
| intracellular growth of mycobacterium tuberculosis after macrophage cell death leads to serial killing of host cells. | a hallmark of pulmonary tuberculosis is the formation of macrophage-rich granulomas. these may restrict mycobacterium tuberculosis (mtb) growth, or progress to central necrosis and cavitation, facilitating pathogen growth. to determine factors leading to mtb proliferation and host cell death, we used live cell imaging to track mtb infection outcomes in individual primary human macrophages. internalization of mtb aggregates caused macrophage death, and phagocytosis of large aggregates was more cy ... | 2017 | 28130921 |
| targeting pknb, an eukaryotic-like serine/threonine protein kinase of mycobacterium tuberculosis with phytomolecules. | tuberculosis (tb), caused by mycobacterium tuberculosis is one of the most lethal communicable disease globally. as per the who global tb report (2015), 9.6 million cases were reported in year 2014 alone. the receptor-like protein kinase, pknb is crucial for sustained mycobacterial growth. therefore, pknb can be a potential target to develop anti-tuberculosis drugs. in present study, we performed a comparative study to investigate binding efficacies of three phytomolecules namely, demethylcalaba ... | 2017 | 28131886 |
| structure-guided, target-based drug discovery - exploiting genome information from hiv to mycobacterial infections. | the use of protein crystallography in structure-guided drug discovery allows identification of potential inhibitor-binding sites and optimisation of interactions of hits and lead compounds with a target protein. an early example of this approach was the use of the structure of hiv protease in designing aids antivirals. more recently, use of structure-guided design with fragment-based drug discovery, which reduces the size of screening libraries by decreasing complexity, has improved ligand effic ... | 2016 | 28132480 |
| siv infection facilitates mycobacterium tuberculosis infection of rhesus macaques. | tuberculosis (tb) is a common opportunistic infection and the leading cause of death for human immunodeficiency virus (hiv)-infected patients. thus, it is necessary to understand the pathogenetic interactions between m.tb and hiv infection. in this study, we examined m.tb and/or simian immunodeficiency virus (siv) infection of chinese rhesus macaques. while there was little evidence that m.tb enhanced siv infection of macaques, siv could facilitate m.tb infection as demonstrated by x-rays, patho ... | 2016 | 28133458 |
| rapid sputum multiplex detection of the m. tuberculosis complex (mtbc) and resistance mutations for eight antibiotics by nucleotide maldi-tof ms. | the increasing incidence of multidrug-resistant (mdr) and extensively drug-resistant (xdr) mycobacterium tuberculosis (mtb) adds further urgency for rapid and multiplex molecular testing to identify the mtb complex and drug susceptibility directly from sputum for disease control. a nucleotide matrix-assisted-laser-desorption-ionization time-of-flight mass spectrometry (maldi-tof ms)-based assay was developed to identify mtb (mtbid panel) and 45 chromosomal mutations for resistance to eight antib ... | 2017 | 28134321 |
| pathogenic mechanisms of intracellular bacteria. | we wished to overview recent data on a subset of epigenetic changes elicited by intracellular bacteria in human cells. reprogramming the gene expression pattern of various host cells may facilitate bacterial growth, survival, and spread. | 2017 | 28134679 |
| docking into mycobacterium tuberculosis thioredoxin reductase protein yields pyrazolone lead molecules for methicillin-resistant staphylococcus aureus. | the thioredoxin/thioredoxin reductase system (trx/trxr) is an attractive drug target because of its involvement in a number of important physiological processes, from dna synthesis to regulating signal transduction. this study describes the finding of pyrazolone compounds that are active against staphylococcus aureus. initially, the project was focused on discovering small molecules that may have antibacterial properties targeting the mycobacterium tuberculosis thioredoxin reductase. this led to ... | 2017 | 28134858 |
| role of reactive oxygen species (ros) in therapeutics and drug resistance in cancer and bacteria. | evading persistent drug resistance in cancer and bacteria is quintessential to restore health in humans, and impels intervention strategies. a distinct property of the cancer phenotype is enhanced glucose metabolism and oxidative stress. reactive oxygen species (ros) are metabolic byproducts of aerobic respiration and are responsible for maintaining redox homeostasis in cells. redox balance and oxidative stress are orchestrated by antioxidant enzymes, reduced thiols and nadp(h) cofactors, which ... | 2017 | 28135088 |
| the role of cysteine residues in catalysis of phosphoenolpyruvate carboxykinase from mycobacterium tuberculosis. | mycobacterium tuberculosis (mtb), the causative agent of tuberculosis, can persist in macrophages for decades, maintaining its basic metabolic activities. phosphoenolpyruvate carboxykinase (pck; ec 4.1.1.32) is a key player in central carbon metabolism regulation. in replicating mtb, pck is associated with gluconeogenesis, but in non-replicating mtb, it also catalyzes the reverse anaplerotic reaction. here, we explored the role of selected cysteine residues in function of mtb pck under different ... | 2017 | 28135343 |
| hypoxia sensing and persistence genes are expressed during the intra-granulomatous survival of m. tuberculosis. | while it is accepted that the environment within the granuloma profoundly affects mycobacterium tuberculosis (mtb) and infection outcome, our ability to understand mtb gene expression in these niches has been limited. we determined intragranulomatous gene expression in human like lung lesions derived from nonhuman primates with both active (atb) and latent (ltbi) tuberculosis. | 2017 | 28135421 |
| analysis of the association between mycobacterium tuberculosis infection and immunoglobulin a nephropathy by early secreted antigenic target 6 detection in renal biopsies: a prospective study. | immunoglobulin a nephropathy (igan) is the most frequent cause of primary renal disease, and clarifying the pathogenesis of igan is of great importance for its diagnosis and treatment. it is well known that mycobacterium tuberculosis (mtb) can infect the urinary tract and result in the typical symptoms of cystitis. however, mtb can also affect the kidney more insidiously. patients may present with glomerular disease, and sometimes with advanced renal failure. this study was to investigate the as ... | 2017 | 28135876 |
| mycobacterial dna replication as a target for antituberculosis drug discovery. | mycobacterium tuberculosis (m. tuberculosis), the causative agent of tuberculosis, is a leading infectious disease organism, causing millions of deaths each year. this serious pathogen has been greatly spread worldwide and recent years have observed an increase in the number of multi-drug resistant and totally drug resistant m. tuberculosis strains (who report, 2014). the danger of tuberculosis becoming an incurable disease has emphasized the need for the discovery of a new generation of antimic ... | 2017 | 28137234 |
| model systems for pulmonary infectious diseases: paradigms of anthrax and tuberculosis. | robert koch utilized animal model systems to put forward his postulates while discovering the etiological agents of anthrax and tuberculosis, bacillus anthracis and mycobacterium tuberculosis, respectively. after more than 130 years, we have achieved limited success towards understanding these two pestilences, which have propagated as scourge against humans. b. anthracis and m. tuberculosis are diverse organisms, which share a common evolutionary path in tropics. they adapt unique strategies to ... | 2017 | 28137237 |
| how can nanoparticles contribute to antituberculosis therapy? | therapeutic approaches using nanoparticles are being successfully used in foods and in several fields of medicine, including infectious diseases. regarding tuberculosis (tb) treatment, nanoparticles can be a useful strategy for two distinct applications: (i) for their intrinsic antimycobacterial activity; (ii) as vehicles for known antitubercular drugs to allow reduction of dose- and drug-associated side-effects and administration via user-friendly administration routes such as pulmonary or oral ... | 2017 | 28137645 |
| some synonymous and nonsynonymous gyra mutations in mycobacterium tuberculosis lead to systematic false-positive fluoroquinolone resistance results with the hain genotype mtbdrsl assays. | in this study, using the hain genotype mtbdrsl assays (versions 1 and 2), we found that some nonsynonymous and synonymous mutations in gyra in mycobacterium tuberculosis result in systematic false-resistance results to fluoroquinolones by preventing the binding of wild-type probes. moreover, such mutations can prevent the binding of mutant probes designed for the identification of specific resistance mutations. although these mutations are likely rare globally, they occur in approximately 7% of ... | 2017 | 28137812 |
| pharmacokinetic modeling and limited sampling strategies based on healthy volunteers for monitoring of ertapenem in patients with multidrug-resistant tuberculosis. | ertapenem is a broad-spectrum carbapenem antibiotic whose activity against mycobacterium tuberculosis is being explored. carbapenems have antibacterial activity when the plasma concentration exceeds the mic at least 40% of the time (40% tmic). to assess the 40% tmic in multidrug-resistant tuberculosis (mdr-tb) patients, a limited sampling strategy was developed using a population pharmacokinetic model based on data for healthy volunteers. a two-compartment population pharmacokinetic model was de ... | 2017 | 28137814 |
| an interactive macrophage signal transduction map facilitates comparative analyses of high-throughput data. | macrophages (mϕs) are key players in the coordination of the lifesaving or detrimental immune response against infections. the mechanistic understanding of the functional modulation of mϕs by pathogens and pharmaceutical interventions at the signal transduction level is still far from complete. the complexity of pathways and their cross-talk benefits from holistic computational approaches. in the present study, we reconstructed a comprehensive, validated, and annotated map of signal transduction ... | 2017 | 28137890 |
| genexpert: a new tool for the rapid detection of rifampicin resistance in mycobacterium tuberculosis. | to evaluate the diagnostic accuracy of genexpert assay for the detection of rifampicin resistance in mycobacterium tuberculosis using conventional drug susceptibility testing as gold standard. | 2017 | 28138184 |
| homology modeling and identification of amino acids involved in the catalytic process of mycobacterium tuberculosis serine acetyltransferase. | serine acetyltransferase (cyse) belongs to the hexapeptide acetyltransferase family and is involved in the biosynthesis of l‑cysteine in microorganisms. mycobacterium tuberculosis cyse is regarded as a potential target for anti‑tuberculosis (tb) drugs; however, the structure and active sites of m. tuberculosis cyse remain unknown. the present study aimed to predict the secondary structure and to construct a 3d model for m. tuberculosis cyse using bioinformatics analysis. to determine the essenti ... | 2017 | 28138713 |
| the diagnostic value of polymerase chain reaction for mycobacterium tuberculosis to distinguish intestinal tuberculosis from crohn's disease: a meta-analysis. | intestinal tuberculosis (itb) and crohn's disease (cd) are important differential diagnoses that can be difficult to distinguish. polymerase chain reaction (pcr) for mycobacterium tuberculosis (mtb) is an efficient and promising tool. this meta-analysis was performed to systematically and objectively assess the potential diagnostic accuracy and clinical value of pcr for mtb in distinguishing itb from cd. | 2017 | 28139494 |
| virulence-dependent induction of interleukin-10-producing-tolerogenic dendritic cells by mycobacterium tuberculosis impedes optimal t helper type 1 proliferation. | mycobacterium tuberculosis inhibits optimal t helper type 1 (th1) responses during infection. however, the precise mechanisms by which virulent m. tuberculosis limits th1 responses remain unclear. here, we infected dendritic cells (dcs) with the virulent m. tuberculosis strain h37rv or the attenuated strain h37ra to investigate the phenotypic and functional alterations in dcs and resultant t-cell responses. h37rv-infected dcs suppressed th1 responses more strongly than h37ra-infected dcs. intere ... | 2017 | 28140445 |
| ethno-medicinal documentation of polyherbal medicines used for the treatment of tuberculosis in amathole district municipality of the eastern cape province, south africa. | tuberculosis (tb) has remained a devastating global public health problem. in the continuing search for effective treatment, polyherbal remedies used as alternative medicines in the eastern cape province of south africa were surveyed. | 2017 | 28140734 |
| mycobacterial phenolic glycolipid synthesis is regulated by camp-dependent lysine acylation of fadd22. | the mycobacterial cell envelope is unique in its chemical composition, and has an important role to play in pathogenesis. phthiocerol dimycocerosates (pdims) and glycosylated phenolphthiocerol dimycocerosates, also known as phenolic glycolipids (pgls), contribute significantly to the virulence of mycobacterium tuberculosis. fadd22 is essential for pgl biosynthesis. we have recently shown in vitro that fadd22 is a substrate for lysine acylation by a unique camp-dependent, protein lysine acyltrans ... | 2017 | 28141495 |
| effect of pregnancy on interferon gamma release assay and tuberculin skin test detection of latent tb infection among hiv-infected women in a high burden setting. | peripartum immunologic changes may affect latent tuberculosis infection (ltbi) diagnostic performance among hiv-infected women. | 2017 | 28141782 |
| a novel benzothiazinethione analogue sklb-tb1001 displays potent antimycobacterial activities in a series of murine models. | new chemotherapeutic compounds and regimens are needed to combat multidrug-resistant mycobacterium tuberculosis. here, we used a series of murine models to assess an antitubercular lead compound sklb-tb1001. in the mycobacterium bovis bacillus calmette-guérin and the acute m. tuberculosis h37rv infection mouse models, sklb-tb1001 significantly attenuated the mycobacterial load in lungs and spleens. the colony forming unit counts and histological examination of lungs from h37rv infected mice reve ... | 2017 | 28142116 |
| mycobacterium tuberculosis virulence-regulator phop interacts with alternative sigma factor sige during acid-stress response. | the ability to sense acid stress and mount an appropriate adaptive response by mycobacterium tuberculosis, which adapts a long-term residence in the macrophage phagosome, remains one of the critical features that defines mycobacterial physiology and its intracellular location. to understand the mechanistic basis of adaptation of the intracellular pathogen, we studied global regulation of m. tuberculosis gene expression in response to acid stress. although recent studies indicate a role for the v ... | 2017 | 28142206 |
| prokaryotic ubiquitin-like protein remains intrinsically disordered when covalently attached to proteasomal target proteins. | the post-translational modification pathway referred to as pupylation marks proteins for proteasomal degradation in mycobacterium tuberculosis and other actinobacteria by covalently attaching the small protein pup (prokaryotic ubiquitin-like protein) to target lysine residues. in contrast to the functionally analogous eukaryotic ubiquitin, pup is intrinsically disordered in its free form. its unfolded state allows pup to adopt different structures upon interaction with different binding partners ... | 2017 | 28143508 |
| identification of mycobacterium tuberculosis in clinical specimens of patients suspected of having extrapulmonary tuberculosis by application of nested pcr on five different genes. | definitive and rapid diagnosis of extrapulmonary tuberculosis (eptb) is challenging since conventional techniques have limitations due to the paucibacillary nature of the disease. to increase the sensitivity of detection of mycobacterium tuberculosis (mtb) in eptb specimens, we performed a nested pcr assay targeting several genes of mtb on eptb specimens. a total of 100 clinical specimens from suspected cases of eptb were processed. standard staining for acid fast bacilli (afb) was performed as ... | 2017 | 28144587 |
| molecular detection of m. tuberculosis and m. bovis and hematological and biochemical analyses in agricultural sprayers exposed to pesticides: a cross-sectional study in punjab, pakistan during 2014-2016. | we determined the correlation between a pesticide exposure, physical health and susceptibility toward tuberculosis along with hematological indices and liver enzymes' alterations in sprayers exposed to pesticides. molecular detection of mycobacterium tuberculosis and mycobacterium bovis was detected by targeting histone-like protein (hupb) gene. the wbc (white blood cells) and rbc (red blood cells) levels of male sprayers and non-sprayers were significantly different (p<0.05). in female spray wo ... | 2017 | 28145400 |
| colonization with helicobacter is concomitant with modified gut microbiota and drastic failure of the immune control of mycobacterium tuberculosis. | epidemiological and experimental observations suggest that chronic microbial colonization can impact the immune control of other unrelated pathogens contracted in a concomitant or sequential manner. possible interactions between mycobacterium tuberculosis infection and persistence of other bacteria have scarcely been investigated. here we demonstrated that natural colonization of the digestive tract with helicobacter hepaticus in mice is concomitant with modification of the gut microbiota, subcl ... | 2017 | 28145441 |
| the antibacterial activities of aditoprim and its efficacy in the treatment of swine streptococcosis. | aditoprim (adp) has potential use as an antimicrobial agent in animals. however, its pharmacodynamic properties have not been systematically studied yet. in this study, the in vitro antibacterial activities of adp and its main metabolites were assayed, and the in vivo antibacterial efficacy of adp for the treatment of swine streptococcosis was evaluated. it was shown that salmonella and streptococcus from swine, escherichia coli and salmonella from chickens, e. coli, streptococcus, mannheimia, p ... | 2017 | 28145487 |
| hepatic tuberculosis in human immunodeficiency virus co-infected adults: a case series of south african adults. | although mycobacterium tuberculosis (tb) infection may cause extrapulmonary disease in hiv-infected adults, hiv-associated hepatic tb has been poorly characterized. our objective was to describe hepatic tb in hiv-infected adults. | 2017 | 28148232 |
| potential of zanthoxylum leprieurii as a source of active compounds against drug resistant mycobacterium tuberculosis. | tuberculosis (tb) is still a global health problem mainly due to development of resistance and co-infection with the human immune virus (hiv). treatment of multi and extensively drug resistant tb requires use of second line drugs which are less efficacious, expensive and very toxic. this has necessitated a need to search for new treatment regimens especially from medicinal plants. zanthoxylum leprieurii, a plant species from rutaceae is used locally in the treatment of tuberculosis in uganda. th ... | 2017 | 28148252 |
| bacterial virulence factors: secreted for survival. | virulence is described as an ability of an organism to infect the host and cause a disease. virulence factors are the molecules that assist the bacterium colonize the host at the cellular level. these factors are either secretory, membrane associated or cytosolic in nature. the cytosolic factors facilitate the bacterium to undergo quick adaptive-metabolic, physiological and morphological shifts. the membrane associated virulence factors aid the bacterium in adhesion and evasion of the host cell. ... | 2017 | 28148975 |
| structural basis for the in vitro known acyl-depsipeptide 2 (adep2) inhibition to clp 2 protease from mycobacterium tuberculosis. | inhibition of mycobacterium tuberculosis clp 2 protease has emerged as an attractive therapeutic option for treatment. acyldepsipeptides (adeps) is known as an inhibitor for clp 2 protease. therefore, it is of interest to document its affinity, enzyme activity and adme profiles. we report the predicted binding affinity of all known clp 2 inhibitors like idr-10001 and idr-10011 against clp2 protease using moldock algorithm aided molecular docking. the predicted activity (using molinspiration serv ... | 2016 | 28149041 |
| in silico identification and characterization of a hypothetical protein of mycobacterium tuberculosis eai5 as a potential virulent factor. | tuberculosis, a life threatening disease caused by different strains of mycobacterium tuberculosis is creating an alarming condition due to the emergence of increasing multi drug resistance (mdr) trait. in this study, in silico approach was used for the identification of a conserved novel virulent factor in mycobacterium tuberculosis eai5 (accession no.cp006578) which can also act as potential therapeutic target. systematic comparative search of genes that are common to strain eai5 and other hum ... | 2016 | 28149053 |
| molecular typing methods used in studies of mycobacterium tuberculosis in iran: a systematic review. | molecular typing methods are important and useful tools to assess the transmission, diversity of strains and differentiation between new infections and relapses which can effectively help in controlling infections. the aim of this study was to evaluate the molecular typing methods which have been used in iran. by evaluating the results and discriminatory power of each method, we can assign appropriate weight to each technique and ultimately offer a common strategy for future epidemiological stud ... | 2016 | 28149495 |
| primary ethambutol resistance among iranian pulmonary tuberculosis patients: a systematic review. | ethambutol (emb) is an anti-mycobacterial agent that is most commonly used in combination with other anti-tuberculosis (tb) drugs in the treatment of tb. studies have shown that primary resistance rates of mycobacterium tuberculosis to emb vary widely, that is, from 1% to as high as 14%. in this study, we aimed to determine the exact prevalence of primary emb resistance among pulmonary tb cases. | 2016 | 28149517 |
| immunological consequences of strain variation within the mycobacterium tuberculosis complex. | in 2015, there were an estimated 10.4 million new cases of tuberculosis (tb) globally, making it one of the leading causes of death due to an infectious disease. tb is caused by members of the mycobacterium tuberculosis complex (mtbc), with human disease resulting from infection by m. tuberculosis sensu stricto and m. africanum. recent progress in genotyping techniques, in particular the increasing availability of whole genome sequence data, has revealed previously under appreciated levels of ge ... | 2017 | 28150302 |
| discovery of anti-microbial and anti-tubercular molecules from fusarium solani: an endophyte of glycyrrhiza glabra. | glycyrrhiza glabra is a high-value medicinal plant thriving in biodiversity rich kashmir himalaya. the present study was designed to explore the fungal endophytes from g. glabra as a source of bioactive molecules. | 2017 | 28150900 |
| miliary tuberculosis: epidemiologicaland clinical analysis of large-case series from moderate to low tuberculosis endemic country. | the aim of this study was to determine the clinical features, and outcome of the patients with miliary tuberculosis (tb).we retrospectively evaluated 263 patients (142 male, 121 female, mean age: 44 years, range: 16-89 years) with miliary tb. criteria for the diagnosis of miliary tb were at least one of the followings in the presence of clinical presentation suggestive of miliary tb such as prolonged fever, night sweats, anorexia, weight loss: radiologic criterion and pathological criterion and/ ... | 2017 | 28151863 |
| the biodistribution of 5-[18f]fluoropyrazinamide in mycobacterium tuberculosis-infected mice determined by positron emission tomography. | 5-[18f]f-pyrazinamide (5-[18f]f-pza), a radiotracer analog of the first-line tuberculosis drug pyrazinamide (pza), was employed to determine the biodistribution of pza using pet imaging and ex vivo analysis. 5-[18f]f-pza was synthesized in 60 min using a halide exchange reaction. the overall decay-corrected yield of the reaction was 25% and average specific activity was 2.6 × 106 kbq (70 mci)/μmol. the biodistribution of 5-[18f]f-pza was examined in a pulmonary mycobacterium tuberculosis mouse m ... | 2017 | 28151985 |
| human immunology of tuberculosis. | immunology is a central theme when it comes to tuberculosis (tb). the outcome of human infection with mycobacterium tuberculosis is dependent on the ability of the immune response to clear or contain the infection. in cases where this fails, the bacterium replicates, disseminates within the host, and elicits a pathologic inflammatory response, and disease ensues. clinical presentation of tb disease is remarkably heterogeneous, and the disease phenotype is largely dependent on host immune status. ... | 2017 | 28155806 |
| metabolic perspectives on persistence. | accumulating evidence has left little doubt about the importance of persistence or metabolism in the biology and chemotherapy of tuberculosis. however, knowledge of the intersection between these two factors has only recently begun to emerge. here, we provide a focused review of metabolic characteristics associated with mycobacterium tuberculosis persistence. we focus on metabolism because it is the biochemical foundation of all physiologic processes and a distinguishing hallmark of m. tuberculo ... | 2017 | 28155811 |
| erythema nodosum and the risk of tuberculosis in a high incidence setting. | objective this study estimates the erythema nodosum (en) incidence in a tuberculosis (tb) endemic setting and evaluates the likelihood of a subsequent tb diagnosis among individuals with mycobacterium tuberculosis infection (mti) with or without en. design we estimated en incidence rates (irs) in east greenland in 2010-2011 and conducted a cohort study following all individuals who tested positive for mti from 1 january 2010 until 31 december 2012. a personal identifier allowed individual follow ... | 2016 | 28156379 |
| drug-resistant tuberculosis in hiv-infected patients in a national referral hospital, phnom penh, cambodia. | background and objective there are no recent data on the prevalence of drug-resistant tuberculosis (dr tb) in cambodia. we aim to describe tb drug resistance amongst adults with pulmonary and extra-pulmonary tb and human immunodeficiency virus (hiv) co-infection in a national referral hospital in phnom penh, cambodia. design between 22 november 2007 and 30 november 2009, clinical specimens from hiv-infected patients suspected of having tb underwent routine microscopy, mycobacterium tuberculosis ... | 2015 | 28156718 |
| planarity of benzoylthiocarbazate tuberculostatics. iii. diesters of 3-(2-hydroxybenzoyl)dithiocarbazic acid. | searches for new tuberculostatic agents are important considering the occurrence of drug-resistant strains of mycobacterium tuberculosis. the structures of three new potentially tuberculostatic compounds, namely isopropyl methyl (2-hydroxybenzoyl)carbonohydrazonodithioate, c12h16n2o2s2, (z)-benzyl methyl (2-hydroxybenzoyl)carbonohydrazonodithioate, c16h16n2o2s2, and dibenzyl (2-hydroxybenzoyl)carbonohydrazonodithioate propan-2-ol monosolvate, c22h20n2o2s2·c3h8o, were determined by x-ray diffract ... | 2017 | 28157125 |
| transmission electron microscopy of xdr mycobacterium tuberculosis isolates grown on high dose of ofloxacin. | the aim of the study was to investigate behavior of resistant mycobacterium tuberculosis (mtb) isolates under a high dose of ofloxacin and its morphological changes. 19 extensively drug resistant (xdr) clinical isolates of mtb were grown on löwenstein-jensen medium containing progressively increasing concentrations of ofloxacin (2, 4, 8, 16, 32 mg/l). ultra-structure analyses of resistant isolates grown on ofloxacin were conducted by transmission electron microscopy (tem). fixation was carried out ... | 2017 | 28157163 |
| synthesis of novel pyrazinamide derivatives based on 3-chloropyrazine-2-carboxamide and their antimicrobial evaluation. | aminodehalogenation of 3-chloropyrazine-2-carboxamide with variously substituted benzylamines yielded a series of fifteen 3-benzylaminopyrazine-2-carboxamides. four compounds possessed in vitro whole cell activity against mycobacterium tuberculosis h37rv that was at least equivalent to that of the standard pyrazinamide. mic values ranged from 6 to 42 μm. the best mic (6 μm) was displayed by 3-[(4-methylbenzyl)amino]pyrazine-2-carboxamide (8) that also showed low cytotoxicity in the hepg2 cell li ... | 2017 | 28157178 |
| profile of tuberculous cervical lymphadenopathy in children. | analyze clinical profile of tuberculous cervical lymphadenopathy in children. | 2017 | 28158382 |
| humoral immune profiling of mycobacterial antigen recognition in sarcoidosis and löfgren's syndrome using high-content peptide microarrays. | sarcoidosis is considered an idiopathic granulomatous disease, although similar immunological and clinical features with tuberculosis (tb) suggest mycobacterial involvement in its pathogenesis. high-content peptide microarrays (hcpm) may help to decipher mycobacteria-specific antibody reactivity in sarcoidosis. | 2017 | 28159576 |
| safety and efficacy of the c-tb skin test to diagnose mycobacterium tuberculosis infection, compared with an interferon γ release assay and the tuberculin skin test: a phase 3, double-blind, randomised, controlled trial. | targeted screening and treatment of mycobacterium tuberculosis infection substantially reduces the risk of developing active tuberculosis. c-tb (statens serum institute, copenhagen, denmark) is a novel specific skin test based on esat-6 and cfp10 antigens. we investigated the safety and diagnostic potential of c-tb compared with established tests in the contact-tracing setting. | 2017 | 28159608 |
| rifz (amed_0655) is a pathway-specific regulator for rifamycin biosynthesis in amycolatopsis mediterranei. | rifamycin and its derivatives are particularly effective against the pathogenic mycobacteria mycobacterium tuberculosis and mycobacterium leprae although the biosynthetic pathway of rifamycin has been extensively studied in amycolatopsis mediterranei, little is known about the regulation in rifamycin biosynthesis. here, an in vivo transposon system was employed to identify genes involved in the regulation of rifamycin production in a. mediterranei u32. in total, nine rifamycin-deficient mutants ... | 2017 | 28159794 |
| rv0774c, an iron stress inducible, extracellular esterase is involved in immune-suppression associated with altered cytokine and tlr2 expression. | tuberculosis, one of the leading cause of death from infectious diseases, is caused by mycobacterium tuberculosis. the genome of m. tuberculosis has been sequenced and nearly 40% of the whole genome sequence was categorized as hypothetical. rv0774c was annotated as membrane exported hypothetical protein in tb database. in silico analysis revealed that rv0774c is a paralog of pe-pgrs multi gene family with 100 aa n-terminal domain similar to pe domain of pe-pgrs proteins. its c-terminal domain is ... | 2017 | 28161108 |
| mycobacterium tuberculosis proteins involved in cell wall lipid biosynthesis improve bcg vaccine efficacy in a murine tb model. | advances in tuberculosis (tb) vaccine development are urgently required to enhance global disease management. we evaluated the potential of mycobacterium tuberculosis (m. tb)-derived protein antigens rv0447c, rv2957 and rv2958c to boost bcg vaccine efficacy in the presence or absence of glucopyranosyl lipid adjuvant formulated in a stable emulsion (gla-se) adjuvant. | 2017 | 28161464 |
| activity of moxifloxacin and linezolid against mycobacterium tuberculosis in combination with potentiator drugs verapamil, timcodar, colistin and sq109. | current treatment for tuberculosis (tb) is complicated by the emergence of multidrug resistant tb (mdr-tb). as a result, there is an urgent need for new powerful anti-tb regimens and novel strategies. in this study, we aimed to potentiate a moxifloxacin + linezolid backbone as treatment for mdr-tb with the efflux pump inhibitors verapamil and timcodar as well as with drugs that act on mycobacterial cell wall stability such as colistin and sq109. using a time-kill kinetics assay, the activities o ... | 2017 | 28162983 |
| the risk of global epidemic replacement with drug-resistant mycobacterium tuberculosis strains. | multidrug-resistant tuberculosis (mdr-tb) is a threat to tuberculosis (tb) control. to guide tb control, it is essential to understand the extent to which and the circumstances in which mdr-tb will replace drug-susceptible tb (ds-tb) as the dominant phenotype. the issue was examined by assessing evidence from genomics, pharmacokinetics, and epidemiology studies. this evidence was then synthesized into a mathematical model. | 2017 | 28163165 |
| experimental animal modelling for tb vaccine development. | research for a novel vaccine to prevent tuberculosis is an urgent medical need. the current vaccine, bcg, has demonstrated a non-homogenous efficacy in humans, but still is the gold standard to be improved upon. in general, the main indicator for testing the potency of new candidates in animal models is the reduction of the bacillary load in the lungs at the acute phase of the infection. usually, this reduction is similar to that induced by bcg, although in some cases a weak but significant impr ... | 2017 | 28163168 |
| hematospermia in a returned traveler. | hematospermia is a common complaint among patients seen in outpatient urology clinics. the differential diagnosis is broad and includes inflammatory, infectious, neoplastic, structural, systemic, and traumatic causes. the most common infectious causes are uropathogens and sexually transmitted infections. however, with increasing global travel, physicians must maintain a high clinical suspicion for pathogens not endemic to their region, including echinococcus, mycobacterium tuberculosis, and schi ... | 2017 | 28163813 |
| aminorifamycins and sporalactams produced in culture by a micromonospora sp. isolated from a northeastern-pacific marine sediment are potent antibiotics. | the new ansa macrolide antibiotics 1 to 4 have been isolated from cultures of a micromonospora sp. obtained from a marine sediment. rifamycins 1 and 2 are the first natural ansa macrolides to have a 3-amino substituent. sporalactams a (3) and b (4) are comprised of a heterocylic core 5 and a 14-membered ansa bridge that are both unprecedented. sporalactam b (4) shows selective and potent inhibition of mycobacterium tuberculosis. | 2017 | 28164711 |
| characterization and function of mycobacterium tuberculosis h37rv lipase rv1076 (lipu). | lipids and lipases/esterases are essential for mycobacterium tuberculosis (mtb) survival and persistence, even virulence. mycobacterium tuberculosis h37rv rv1076 (lipu), a member of lipase family, is homologous to the human hormone sensitive lipase (hsl) based on the presence of conserved motif 'gxsxg'. to define the enzymatic characteristics of rv1076, the gene was cloned, and expressed in escherichia coli. the protein was purified for enzymatic characterization. lipu showed high specific activ ... | 2017 | 28164792 |
| oral recombinant human or mouse lactoferrin reduces mycobacterium tuberculosis tdm induced granulomatous lung pathology. | trehalose 6'6-dimycolate (tdm) is the most abundant glycolipid on the cell wall of mycobacterium tuberculosis (mtb). tdm is capable of inducing granulomatous pathology in mouse models that resembles those induced by mtb infection. using the acute tdm model, this work investigates the effect of recombinant human and mouse lactoferrin to reduce granulomatous pathology. c57bl/6 mice were injected intravenously with tdm at a dose of 25 μg·mouse(-1). at day 4 and 6, recombinant human or mouse lactofe ... | 2017 | 28165282 |
| programmable transcriptional repression in mycobacteria using an orthogonal crispr interference platform. | the development of new drug regimens that allow rapid, sterilizing treatment of tuberculosis has been limited by the complexity and time required for genetic manipulations in mycobacterium tuberculosis. crispr interference (crispri) promises to be a robust, easily engineered and scalable platform for regulated gene silencing. however, in m. tuberculosis, the existing streptococcus pyogenes cas9-based crispri system is of limited utility because of relatively poor knockdown efficiency and proteot ... | 2017 | 28165460 |
| correction: evaluation of direct colorimetric mtt assay for rapid detection of rifampicin and isoniazid resistance in mycobacterium tuberculosis. | [this corrects the article doi: 10.1371/journal.pone.0169188.]. | 2017 | 28166294 |
| bayesian population structure analysis reveals presence of phylogeographically specific sublineages within previously ill-defined t group of mycobacterium tuberculosis. | mycobacterium tuberculosis genetic structure, and evolutionary history have been studied for years by several genotyping approaches, but delineation of a few sublineages remains controversial and needs better characterization. this is particularly the case of t group within lineage 4 (l4) which was first described using spoligotyping to pool together a number of strains with ill-defined signatures. although t strains were not traditionally considered as a real phylogenetic group, they did contai ... | 2017 | 28166309 |
| risk factors for readmission to hospital in patients with tuberculosis in tehran, iran: three-year surveillance. | tuberculosis (tb) is still a major health problem and tb hospital readmission could increase health system costs. in a retrospective study in a tertiary referral hospital for tb in tehran, iran, tb patients with readmission were evaluated. these tb patients in the index year who were then readmitted were compared with tb patients in the same year who were not readmitted during the follow-up period. one hundred and forty-six patients had hospital readmission within three-year follow-up with mean ... | 2017 | 28166697 |
| two cases of eyelid tuberculosis - an uncommon presentation of ocular tuberculosis. | mycobacterium tuberculosis apart from being the causative agent of pulmonary tuberculosis is also notorious to cause tuberculosis at various sites in the human body and ocular tuberculosis is one of the extra pulmonary manifestations of this organism. the most common presentation of ocular tuberculosis is anterior uveitis or choroiditis caused by hematogenous infection or hypersensitivity after another organ infection. eyelid involvement by tuberculosis is most of the times secondary to orbital ... | 2017 | 28166917 |