Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| detection of salmonella in finishing pigs on farm and at slaughter in piedmont, italy. | salmonella is one of the most common causes of human gastroenteritis often associated with pork consumption. the aims of this cross-sectional study were to collect preliminary data on the presence of salmonella enterica in pigs in piedmont (italy), through sampling on farm and at slaughter and to gather pilot data on serotypes and phagetypes present in the sampled area and distribution of anti-microbial resistance among isolated strains. salmonella was detected through culture and identified wit ... | 2009 | 19068072 |
| isolasion, identification and antimicrobial resistance patterns of salmonella from meat products in tehran. | the present study was carried out from 400 samples in different slaughterhouses to report the isolation along with the serotypes and antibiogram pattern of salmonella among products in tehran. salmonella was isolated from samples of chicken, beef; veal, mutton, roast beef and sausage fermentive meat collected at slaughterhouses. the isolates were characterized by serotyping and antimicrobial-susceptibility testing. eighty isolates of salmonella enterica belonging to 19 serotypes--s. adelaide, s. ... | 2007 | 19069997 |
| variable number of tandem repeats (vntr) and its application in bacterial epidemiology. | molecular epidemiology is the using of molecular techniques to study bacterial distribution in human populations. recently molecular epidemiologist benefit from several techniques such as variable number tandem repeat (vntr) typing method to typing bacterial strains. variable number tandem repeat (vntr) typing is a tool for genotyping and provides data in a simple and numeric format based on the number of repetitive sequences. vntr for first time identified in m. tuberculosis as mycobacterial in ... | 2007 | 19070072 |
| the immunobiology of avian systemic salmonellosis. | avian systemic salmonellosis is primarily caused by salmonella enterica serovar gallinarum and serovar pullorum causing the diseases fowl typhoid and pullorum disease respectively. during infection interaction with the immune system occurs in three main phases. first is invasion via the gastrointestinal tract. infection with s. pullorum or s. gallinarum does not cause substantial inflammation, unlike s. typhimurium or s. enteritidis. through in vitro models it was found that s. gallinarum does n ... | 2009 | 19070366 |
| oral delivery of dna vaccine encoding vp28 against white spot syndrome virus in crayfish by attenuated salmonella typhimurium. | protective immune responses in shrimp induced by dna vaccines against white spot syndrome virus (wssv) with intramuscular injection have been reported in recent reports. in this study, we investigated the utilities of attenuated salmonella enterica serovar typhimurium (salmonella typhimurium) as a bactofection vehicle for the oral delivery of a dna vaccine plasmid to crayfish (cambarus clarkii). the dna vaccine plasmid pcdna3.1-vp28, encoding viral envelope protein vp28, was transformed to an at ... | 2009 | 19071178 |
| outbreak of salmonella enterica serotype montevideo infections in france linked to consumption of cheese made from raw milk. | in 2006, an outbreak of salmonella enterica serotype montevideo infections occurred in france. a matched case-control study and microbiological, environmental, and veterinary investigations were conducted to determine the source of this outbreak. a case was defined as a resident of france in whom salmonella montevideo was isolated from a stool or blood specimen between october 16, 2006, and january 6, 2007. patients were interviewed using a standardized questionnaire. salmonella montevideo food ... | 2009 | 19072083 |
| bacterial dna topology and infectious disease. | the gram-negative bacterium escherichia coli and its close relative salmonella enterica have made important contributions historically to our understanding of how bacteria control dna supercoiling and of how supercoiling influences gene expression and vice versa. now they are contributing again by providing examples where changes in dna supercoiling affect the expression of virulence traits that are important for infectious disease. available examples encompass both the earliest stages of pathog ... | 2009 | 19073701 |
| molecular characterization and antimicrobial susceptibility of salmonella isolates from infections in humans in henan province, china. | we characterized 208 human salmonella isolates from 2006 to 2007 and 27 human salmonella enterica serovar typhimurium isolates from 1987 to 1993 from henan province, china, by serotyping, by antimicrobial susceptibility testing, and, for the most common serovars, by pulsed-field gel electrophoresis (pfge). the most common serovars among the 2006-2007 isolates were s. enterica serovar typhimurium (27%), s. enterica serovar enteritidis (17%), s. enterica serovar derby (10%), s. enterica serovar in ... | 2009 | 19073874 |
| leucine-responsive regulatory protein (lrp) acts as a virulence repressor in salmonella enterica serovar typhimurium. | leucine-responsive regulatory protein (lrp) is a global gene regulator that influences expression of a large number of genes including virulence-related genes in escherichia coli and salmonella. no systematic studies examining the regulation of virulence genes by lrp have been reported in salmonella. we report here that constitutive expression of lrp [lrp(con)] dramatically attenuates salmonella virulence while an lrp deletion (deltalrp) mutation enhances virulence. the lrp(con) mutant caused pl ... | 2009 | 19074398 |
| association of is26-composite transposons and complex in4-type integrons generates novel multidrug resistance loci in salmonella genomic island 1. | clinical isolates of salmonella enterica serovar haifa and newport, which displayed extended multidrug resistance phenotypes, were investigated for the presence of salmonella genomic island 1 (sgi1) and the genetic organization of its antibiotic resistance gene clusters. | 2009 | 19074421 |
| identification and characterization of class 1 integron resistance gene cassettes among salmonella strains isolated from imported seafood. | a total of 210 salmonella isolates, representing 64 different serovars, were isolated from imported seafood samples, and 55/210 isolates were found to be resistant to at least one antibiotic. class 1 integrons from three multidrug-resistant salmonella enterica strains (salmonella enterica serovars newport [strain 62], typhimurium var. copenhagen [strain 629], and lansing [strain 803], originating from hong kong, the philippines, and taiwan, respectively) were characterized. southern hybridizatio ... | 2009 | 19074612 |
| a mixture containing galactooligosaccharide, produced by the enzymic activity of bifidobacterium bifidum, reduces salmonella enterica serovar typhimurium infection in mice. | the prebiotic bimuno is a mixture containing galactooligosaccharide, produced by the galactosyltransferase activity of bifidobacterium bifidum ncimb 41171 in the presence of lactose. previous studies have implicated prebiotics in reducing infections by enteric pathogens, thus it was hypothesized that bimuno may confer some protection in the murine host from salmonella enterica serovar typhimurium (s. typhimurium) infection. in this study, infection caused by s. typhimurium sl1344nal(r) in the pr ... | 2009 | 19074651 |
| characterization of bacteriophages used in the salmonella enterica serovar enteritidis phage-typing scheme. | the 16 salmonella enterica serovar enteritidis (s. enteritidis) typing phages (setps) used in the laboratory of enteric pathogens (health protection agency, london, uk) phage-typing scheme have not previously been characterized in detail. we have examined the adsorption properties of the phages with respect to a number of s. enterica serovars and defined phage morphology with electron microscopy. pfge was used to estimate overall genome size and banding patterns generated by electrophoresis foll ... | 2009 | 19074657 |
| downregulation of rpon-controlled genes protects salmonella cells from killing by the cationic antimicrobial peptide polymyxin b. | salmonella enterica polymyxin b (pm) resistance is modulated mainly by substitutions of the acyl chains and the phosphate groups on the lipid a moiety of lipopolysaccharide. these modifications are mediated by genes under the control of the pmra/pmrb and phop/phoq two-component regulatory systems. in this study, a deletion in the gene encoding the alternative sigma(54) factor, rpon, was shown to increase pm resistance without affecting protamine sensitivity. the results presented here showed tha ... | 2009 | 19076233 |
| subversion of antimicrobial calprotectin (s100a8/s100a9 complex) in the cytoplasm of tr146 epithelial cells after invasion by listeria monocytogenes. | expressed by squamous mucosal keratinocytes, calprotectin is a complex of two ef-hand calcium-binding proteins of the s100 subfamily (s100a8 and s100a9) with significant antimicrobial activity. calprotectin-expressing cells resist invasion by porphyromonas gingivalis, listeria monocytogenes, and salmonella enterica serovar typhimurium (s. typhimurium). to understand the interactions between calprotectin and invasive bacteria, we studied the distribution of calprotectin in the cytoplasm of tr146 ... | 2009 | 19079333 |
| a dynamic view of the spread and intracellular distribution of salmonella enterica. | the events that determine the dynamics of proliferation, spread and distribution of microbial pathogens within their hosts are surprisingly heterogeneous and poorly understood. we contend that understanding these phenomena at a sophisticated level with the help of mathematical models is a prerequisite for the development of truly novel, targeted preventative measures and drug regimes. we describe here recent studies of salmonella enterica infections in mice which suggest that bacteria resist the ... | 2009 | 19079353 |
| granulomatous myocarditis and coelomic effusion due to salmonella enterica arizonae in a madagascar dumerili's boa (acrantophis dumerili, jan. 1860). | a granulomatous myocarditis due to salmonella enterica arizonae was diagnosed in an 8-year-old madagascar dumerili's boa (acrantophis dumerili) based on positive coelomic effusion culture, ultrasound visualization of abnormal ventricular myocardium, necropsy and cardiac histological examination. | 2003 | 19081357 |
| molecular characterisation of class 1 integrons in salmonella enterica serovar choleraesuis isolates from southern taiwan. | integron-mediated multidrug resistance in salmonellaenterica serovar choleraesuis poses a major concern in human salmonellosis in taiwan. in this study, 71 strains of s. choleraesuis harbouring a class 1 integron from humans and animals were characterised for detection of integrons, gene cassettes and the spvc virulence gene using molecular genetic techniques. all 71 strains tested were negative for class 2 and 3 integrons. fifty-eight (81.7%) of the isolates harboured a class 1 integron with di ... | 2009 | 19084383 |
| genomic analysis of bacteriophage epsilon 34 of salmonella enterica serovar anatum (15+). | the presence of prophages has been an important variable in genetic exchange and divergence in most bacteria. this study reports the determination of the genomic sequence of salmonella phage epsilon 34, a temperate bacteriophage that was important in the early study of prophages that modify their hosts' cell surface and is of a type (p22-like) that is common in salmonella genomes. | 2008 | 19091116 |
| analysis of the role of 13 major fimbrial subunits in colonisation of the chicken intestines by salmonella enterica serovar enteritidis reveals a role for a novel locus. | salmonella enterica is a facultative intracellular pathogen of worldwide importance. over 2,500 serovars exist and infections in humans and animals may produce a spectrum of symptoms from enteritis to typhoid depending on serovar- and host-specific factors. s. enteritidis is the most prevalent non-typhoidal serovar isolated from humans with acute diarrhoeal illness in many countries. human infections are frequently associated with direct or indirect contact with contaminated poultry meat or eggs ... | 2008 | 19091138 |
| a simple screen to identify promoters conferring high levels of phenotypic noise. | genetically identical populations of unicellular organisms often show marked variation in some phenotypic traits. to investigate the molecular causes and possible biological functions of this phenotypic noise, it would be useful to have a method to identify genes whose expression varies stochastically on a certain time scale. here, we developed such a method and used it for identifying genes with high levels of phenotypic noise in salmonella enterica ssp. i serovar typhimurium (s. typhimurium). ... | 2008 | 19096504 |
| characterization of antimicrobial resistance in salmonella enterica serotype heidelberg isolated from food animals. | fifty-eight salmonella enterica serovar heidelberg isolates isolated from food animals were tested for antimicrobial susceptibilities and further characterized for select antimicrobial resistance genes, plasmid carriage, class 1 integrons, and genetic relatedness using pulsed-field gel electrophoresis (pfge). seventy-two percent of isolates displayed resistance to at least one of the antimicrobial agents tested, while 24% exhibited resistance to eight or more antimicrobial agents. resistance was ... | 2009 | 19099358 |
| mucosal immunization with attenuated salmonella enterica serovar typhi expressing protective antigen of anthrax toxin (pa83) primes monkeys for accelerated serum antibody responses to parenteral pa83 vaccine. | salmonella enterica serovar typhi vaccine strain cvd 908-htra was genetically engineered for stable plasmid-based expression of protective antigen of anthrax toxin (pa83) fused with the export protein clya (clya-pa83). the priming potential of cvd 908-htra expressing clya-pa83 was assessed in 12 rhesus and 20 cynomolgus macaques that were immunized mucosally (i.e., intranasally) on days 0 and 14. a parenteral booster with purified pa83 plus alum was given to rhesus macaques on days 42 and 225; c ... | 2009 | 19099487 |
| [invasive salmonella dublin infection in a case]. | 2008 | 19099872 | |
| [increased liver enzymes and fever]. | 2008 | 19100197 | |
| salmonella-containing vacuoles display centrifugal movement associated with cell-to-cell transfer in epithelial cells. | intracellular salmonella enterica serovar typhimurium (serovar typhimurium) occupies a salmonella-containing vacuole (scv) where bacterial effector proteins are secreted into the host cell using type iii secretion systems (t3ss). cytoskeletal motor proteins and t3ss-delivered effector proteins facilitate scv positioning to juxtanuclear positions where bacterial replication occurs. here, we show that this characteristic scv positioning is not maintained by all scvs during infection of hela cells. ... | 2009 | 19103768 |
| salmonella enterica serovar typhimurium strains with regulated delayed attenuation in vivo. | recombinant bacterial vaccines must be fully attenuated for animal or human hosts to avoid inducing disease symptoms while exhibiting a high degree of immunogenicity. unfortunately, many well-studied means for attenuating salmonella render strains more susceptible to host defense stresses encountered following oral vaccination than wild-type virulent strains and/or impair their ability to effectively colonize the gut-associated and internal lymphoid tissues. this thus impairs the ability of reco ... | 2009 | 19103774 |
| multiple regulatory pathways associated with high-level ciprofloxacin and multidrug resistance in salmonella enterica serovar enteritidis: involvement of rama and other global regulators. | mechanisms of antibiotic resistance were examined in nalidixic acid-resistant salmonella enterica serovar enteritidis field isolates displaying decreased susceptibility to ciprofloxacin and in in vitro-derived ciprofloxacin-resistant mutants (104-cip and 5408-cip). all field isolates harbored a single gyra mutation (d87y). deletion of acrb and complementation with wild-type gyra increased quinolone susceptibility. selection for ciprofloxacin resistance was associated with the development of an a ... | 2009 | 19104017 |
| cmy-31 and cmy-36 cephalosporinases encoded by cole1-like plasmids. | two cmy-2 derivatives, cmy-31 (gln(215)-->arg) from salmonella enterica serotype newport and cmy-36 (ala(77)-->cys and gln(193)-->glu) from klebsiella pneumoniae, were characterized. both cephalosporinases functionally resembled cmy-2. bla(cmy) alleles occurred as parts of a putative transposon comprising isecp1b and a citrobacter freundii-derived sequence carried by cole1-like plasmids similar to cmy-5-encoding ptkh11 from klebsiella oxytoca. | 2009 | 19104021 |
| reaction of adomet with thic generates a backbone free radical. | thic is an [4fe-4s] cluster protein that catalyzes the formation of 4-amino-5-hydroxymethyl-2-methylpyrimidine. epr spectroscopic studies demonstrate that, upon interaction with adomet, active thic from salmonella enterica generates a persistent free radical on the alpha-carbon of an amino acid residue. the epr properties of the radical are consistent with any residue other than a gly or ala. exposure to oxygen was accompanied by a fission of the radical-carrying polypeptide chain between the gl ... | 2009 | 19113839 |
| salmonella enterica typhimurium sipa induces cxc-chemokine expression through p38mapk and jun pathways. | the role of salmonella typhimurium type iii secretion system (t3ss-1)-translocated proteins in chemokines' expression and protein phosphorylation was investigated in hela cells. infection of hela cells with s. typhimurium activated il-8 and gro-alpha expression at higher levels than infection with a s. typhimurium sipasopabde2 mutant, confirming that t3ss-1-secreted proteins are required to fully induce chemokine expression in hela cells. a s. typhimurium sipasopabde2 mutant complemented with si ... | 2009 | 19114119 |
| archaeal apbc/nbp35 homologs function as iron-sulfur cluster carrier proteins. | iron-sulfur clusters may have been the earliest catalytic cofactors on earth, and most modern organisms use them extensively. although members of the archaea produce numerous iron-sulfur proteins, the major cluster assembly proteins found in the bacteria and eukarya are not universally conserved in archaea. free-living archaea do have homologs of the bacterial apbc and eukaryotic nbp35 genes that encode iron-sulfur cluster carrier proteins. this study exploits the genetic system of salmonella en ... | 2009 | 19114487 |
| role of the hya hydrogenase in recycling of anaerobically produced h2 in salmonella enterica serovar typhimurium. | double and triple uptake-type hydrogenase mutants were used to determine which hydrogenase recycles fermentatively produced hydrogen. the deltahyb deltahya and deltahyd deltahya double mutants evolved h(2) at rates similar to that of the triple mutant strain, so hya alone oxidizes the bulk of h(2) produced during fermentation. when only hya was present, no hydrogen production was observed in nutrient-limited medium. h(2) uptake assays showed that hya can oxidize both exogenously added h(2) and f ... | 2009 | 19114523 |
| poultry-associated salmonella enterica subsp. enterica serovar 4,12:d:- reveals high clonality and a distinct pathogenicity gene repertoire. | a european baseline survey during the years 2005 and 2006 has revealed that the monophasic salmonella enterica subsp. enterica serovar 4,12:d:- was, with a prevalence of 23.6%, the most frequently isolated serovar in german broiler flocks. in denmark and the united kingdom, its serovar prevalences were 15.15% and 2.8%, respectively. although poultry is a major source of human salmonellosis, serovar 4,12:d:- is rarely isolated in humans (approximately 0.09% per year). molecular typing studies usi ... | 2009 | 19114530 |
| evaluation of new generation salmonella enterica serovar typhimurium vaccines with regulated delayed attenuation to induce immune responses against pspa. | increasing the immunogenicity to delivered antigens by recombinant attenuated salmonella vaccines (rasv) has been the subject of intensive study. with this goal in mind, we have designed and constructed a new generation of rasv that exhibit regulated delayed attenuation. these vaccine strains are phenotypically wild type at the time of immunization and become attenuated after colonization of host tissues. the vaccine strains are grown under conditions that allow expression of genes required for ... | 2009 | 19114649 |
| variation in antimicrobial resistance in sporadic and outbreak-related salmonella enterica serovar typhimurium. | the prevalence of different antimicrobial resistance profiles and variants of the salmonella genomic island 1 (sgi1) was reported for salmonella enterica serovar typhimurium dt104 strains isolated from patients in denmark. variation in antimicrobial resistance and corresponding changes of sgi1 were shown among isolates from a foodborne outbreak. | 2009 | 19116064 |
| deletion of tola in salmonella typhimurium generates an attenuated strain with vaccine potential. | the gram-negative tol-pal system of envelope proteins plays a key role in maintaining outer membrane integrity and contributes to the virulence of several pathogens. we have investigated the role of one of these proteins, tola, in the biology of salmonella enterica serovar typhimurium. deletion of tola rendered strain sl1344 more susceptible to killing by bile and human serum. in addition the mutant had impaired membrane integrity and displayed alterations in lps production. the tola mutant was ... | 2009 | 19118362 |
| osmoregulated periplasmic glucans of salmonella enterica serovar typhimurium are required for optimal virulence in mice. | we purified osmoregulated periplasmic glucans (opgs) from salmonella enterica serovar typhimurium and found them to be composed of 100 % glucose with 2-linked glucose as the most abundant residue, with terminal glucose, 2,3-linked and 2,6-linked glucose also present in high quantities. the two structural genes for opg biosynthesis, opgg and opgh, form a bicistronic operon, and insertion of a kanamycin resistance gene cassette into this operon resulted in a strain devoid of opgs. the opggh mutant ... | 2009 | 19118363 |
| oxidative stress and disruption of labile iron generate specific auxotrophic requirements in salmonella enterica. | the response of a cell to integrated stresses was investigated using environmental and/or genetic perturbations that disrupted labile iron homeostasis and increased oxidative stress. the effects of the perturbations were monitored as nutritional requirements, and were traced to specific enzymic targets. a yggx gsha cyay mutant strain required exogenous thiamine and methionine for growth. the thiamine requirement, which had previously been linked to the fe-s cluster proteins thih and thic, was re ... | 2009 | 19118370 |
| citrate-mediated iron uptake in pseudomonas aeruginosa: involvement of the citrate-inducible feca receptor and the feob ferrous iron transporter. | in an attempt to identify components of a ferric citrate uptake system in pseudomonas aeruginosa, a mutant library of a siderophore-deficient strain (ia614) was constructed and screened for defects in citrate-promoted growth in an fe-restricted medium. a mutant disrupted in gene pa3901, encoding a homologue of the outer-membrane ferric citrate receptor, feca, of escherichia coli (feca(e.c.)), was recovered and shown to be deficient in citrate-promoted growth and citrate-mediated fe uptake. a mut ... | 2009 | 19118371 |
| evaluation of the control of pathogen load by an anti-salmonella bacterium in a herd of cattle with persistent salmonella infection. | to determine whether an anti-salmonella bacterium is involved in control of pathogen load in persistently infected cattle herds. | 2009 | 19119953 |
| regulators of g-protein signalling are modulated by bacterial lipopeptides and lipopolysaccharide. | regulators of g-protein signalling accelerate the gtpase activity of g(alpha) subunits, driving g proteins in their inactive gdp-bound form. this property defines them as gtpase activating proteins. here the effect of different toll-like receptor agonists on rgs1 and rgs2 expression in murine bone marrow-derived macrophages and j774 cells was analysed. after stimulation with tlr2/1 or tlr2/6 lipopeptide ligands and the tlr4/md2 ligand lipopolysaccharide, microarray analyses show only modulation ... | 2009 | 19120454 |
| regulation of mara, soxs, rob, acrab and micf in salmonella enterica serovar typhimurium. | importance of the overexpression of acrab efflux pumps in the low-level resistance of pathogens to antimicrobials requires a better understanding of the acrab regulation. the goal of the present research was to study the transcription of acrab, as well as the genes that play a role in its regulation in salmonella enterica serovar typhimurium. we monitored the transcription of these genes during growth at 30 degrees c and 37 degrees c, and thoroughly studied the effect of salicylate, paraquat and ... | 2008 | 19120970 |
| genetic stability of vaccine strain salmonella typhi ty21a over 25 years. | the attenuated live bacterial vaccine strain salmonella enterica serovar typhi ty21a is the main constituent of vivotif, the only licensed oral vaccine against typhoid fever. the strain was developed in the 1970s by chemical mutagenesis. in the course of this mutagenesis, a number of mutations were introduced into the vaccine strain. characterisation of the vaccine strain during development as well as release of master- and working seed lots (msl and wsl) and commercial batches is based on pheno ... | 2009 | 19121604 |
| calprotectin s100a9 calcium-binding loops i and ii are essential for keratinocyte resistance to bacterial invasion. | epithelial cells expressing calprotectin, a heterodimer of s100a8 and s100a9 proteins, are more resistant to bacterial invasion. to determine structural motifs that affect resistance to bacterial invasion, mutations were constructed in s100a9 targeting the calcium-binding loops i and ii (e36q, e78q, e36q,e78q) and the c terminus (s100a9(1-99) and s100a9(1-112)), which contains putative antimicrobial zinc-binding and phosphorylation sites. the s100a8 and mutated s100a9 encoding plasmids were tran ... | 2009 | 19122197 |
| phage type and antimicrobial susceptibility of salmonella enterica serovar enteritidis from food-producing animals in japan between 1976 and 2004. | a total of 56 isolates of salmonella enterica serovar enteritidis, including 38 isolates from poultry, 16 from cattle and two from pigs, collected between 1976 and 2004, were subjected to bacteriophage typing and antimicrobial susceptibility testing. phage type (pt) 8 was predominant in bovine isolates, whereas pt1 and pt4 were predominant in poultry isolates. resistance was found for 8 of 11 antimicrobials tested, at the following rates: 46.4% for dihydrostreptomycin followed by ampicillin and ... | 2008 | 19123313 |
| genome expression analyses revealing the modulation of the salmonella rcs regulon by the attenuator igaa. | intracellular growth attenuator a (igaa) was identified as a salmonella enterica regulator limiting bacterial growth inside fibroblasts. genetic evidence further linked igaa to repression of the rcscdb regulatory system, which responds to envelope stress. how igaa attenuates this system is unknown. here, we present genome expression profiling data of s. enterica serovar typhimurium igaa mutants grown at high osmolarity and displaying exacerbated rcs responses. transcriptome data revealed that ig ... | 2009 | 19124574 |
| characterization of a monoclonal antibody directed against salmonella enterica serovar typhimurium and serovar [4,5,12:i:-]. | flagellar extracts of salmonella enterica serovars expressing phase 2 h1 antigenic complex (h:1,2, h:1,5, h:1,6, and h:1,7) and a mutant flagellin obtained by site-directed mutagenesis of the fljb gene from serovar typhimurium at codon 218, transforming threonine to alanine, expressed in escherichia coli (fljb218(a)) were used to analyze the h1 antigenic complex. cross-reactions were detected by western blotting and dot blotting using commercial polyclonal antibodies against the different wild-t ... | 2009 | 19124586 |
| distribution, diversity, and seasonality of waterborne salmonellae in a rural watershed. | salmonella outbreaks from contaminated water and nonanimal foods (e.g., produce) are increasingly reported. to address the environment as a potential source of pathogenic salmonella, we investigated levels of salmonellae and the geographic and temporal variation of salmonella serotypes from surface waters in a region of georgia (united states) with a history of high salmonellosis case rates. monthly water samples were collected from six stations in the little river (upper suwannee basin) for 12 ... | 2009 | 19124594 |
| interleukin-17 in host defence against bacterial, mycobacterial and fungal pathogens. | the mammalian immune system is intricately regulated, allowing for potent pathogen-specific immunity to be rapidly activated in response to infection with a broad and diverse array of potential pathogens. as a result of their ability to differentiate into distinct effector lineages, cd4 t cells significantly contribute to pathogen-specific adaptive immune responses. through the production of effector cytokines, cd4 t helper (th) cells orchestrate the precise mobilization of specific immune cells ... | 2009 | 19125888 |
| the salmonella pathogenicity island (spi) 1 contributes more than spi2 to the colonization of the chicken by salmonella enterica serovar typhimurium. | salmonella enterica serovar typhimurium (typhimurium) is an important pathogen that infects a broad range of hosts. in humans, typhimurium causes a gastroenteritis characterized by vomiting, diarrhea, and abdominal pains. typhimurium infection occurs mainly through the ingestion of contaminated food including poultry, pork, eggs, and milk. chickens that are asymptomatic carriers of typhimurium constitute a potential reservoir for infection. the type three secretion systems encoded by salmonella ... | 2009 | 19126220 |
| structural and functional analysis of the c-terminal dna binding domain of the salmonella typhimurium spi-2 response regulator ssrb. | in bacterial pathogenesis, virulence gene regulation is controlled by two-component regulatory systems. in escherichia coli, the envz/ompr two-component system is best understood as regulating expression of outer membrane proteins, but in salmonella enterica, ompr activates transcription of the ssra/b two-component system located on salmonella pathogenicity island 2 (spi-2). the response regulator ssrb controls expression of a type iii secretory system in which effectors modify the vacuolar memb ... | 2009 | 19126546 |
| distribution of antibody titer against salmonella enterica among healthy individuals in nepal. | enteric fever is an endemic problem in nepal and widal agglutination test is widely used for its diagnosis but a normal baseline titer in healthy population and cutoff values have not been established. | 2009 | 19128506 |
| changes in antimicrobial resistance among salmonella enterica subspecies enterica serovar pullorum isolates in china from 1962 to 2007. | there are few data available for the trends of antimicrobial resistance of salmonella enterica subspecies enterica serovar pullorum (s. pullorum) in china and other parts of the world. thus, the objective of this study was to evaluate the changes in antimicrobial resistance of s. pullorum isolated from diseased chickens in china from 1962 to 2007. a total of 450 s. pullorum isolates were tested for their susceptibility to 17 antimicrobials in a disk diffusion method. 39-95% of the isolates displ ... | 2009 | 19128897 |
| bacterial particle endocytosis by epithelial cells is selective and enhanced by tumor necrosis factor receptor ligands. | bacterial pathogens use virulence strategies to invade epithelial barriers, but active processes of epithelial cells may also contribute to the endocytosis of microbial particles. to focus on the latter, we studied the uptake of fixed and fluorescently labeled bacterial particles in intestinal and bronchoepithelial cell cultures and found it to be enhanced in caco-2bbe and nci-h292 cells after treatment with tumor necrosis factor alpha and an agonist antibody against the lymphotoxin beta recepto ... | 2009 | 19129472 |
| discovery of novel 2',3',4'-trihydroxy-2-phenylacetophenone derivatives as anti-gram-positive antibacterial agents. | a number of 2',3',4'-trihydroxy-2-phenylacetophenone derivatives were synthesized and examined for growth inhibition of several kinds of bacteria. 2',3',4'-trihydroxy-2-phenylacetophenone itself exhibited no antibacterial activity, but some of its derivatives showed various antibacterial activities depending on functional groups introduced on the 2-phenyl ring. eighteen out of 24 compounds synthesized in this study appeared to possess antibacterial activities against at least two gram-positive s ... | 2009 | 19129641 |
| a sensitive liquid chromatography/mass spectrometry-based assay for quantitation of amino-containing moieties in lipid a. | a novel sensitive liquid chromatography/mass spectrometry-based assay was developed for the quantitation of aminosugars, including 2-amino-2-deoxyglucose (glucosamine, glcn), 2-amino-2-deoxygalactose (galactosamine, galn), and 4-amino-4-deoxyarabinose (aminoarabinose, aran), and for ethanolamine (etn), present in lipid a. this assay enables the identification and quantitation of all amino-containing moieties present in lipopolysaccharide or lipid a from a single sample. the method was applied to ... | 2009 | 19130491 |
| tlr2 and tlr4 signaling shapes specific antibody responses to salmonella typhi antigens. | tlr directly induce innate immune responses by sensing a variety of microbial components and are critical for the fine-tuning of subsequent adaptive immune responses. however, their impact and mechanism of action on antibody responses against bacterial antigens are not yet fully understood. salmonella enterica serovar typhi (s. typhi) porins have been characterized as inducers of long-lasting specific antibody responses in mice. in this report, we show that immunization of tlr4-deficient (tlr4(- ... | 2009 | 19130558 |
| is rotavirus contributing to an increase of diarrhoea in a region of spain? | diarrhoeal illnesses are the most frequent of notifiable diseases in aragon. physicians notify diarrhoea cases with presumed infectious origin on a weekly basis. following an increase in 2005-2006, we aimed to identify the responsible organism(s) in order to inform control measures. we described seasonality of diarrhoea notifications for 1998-2004 and 2005-2006. we calculated correlations between diarrhoea notifications and enteric pathogens diagnosed in two aragonese laboratories, and applied l ... | 2009 | 19134236 |
| involvement of the cra global regulatory protein in the expression of the iscrsua operon, revealed during studies of tricarballylate catabolism in salmonella enterica. | in salmonella enterica, tricarballylate (tcb) catabolism requires function of tcub, a membrane-bound protein that contains [4fe-4s] clusters and heme. tcub transfers electrons from reduced flavin adenine dinucleotide in the tcb dehydrogenase (tcua) to electron acceptors in the membrane. we recently showed that functions needed to assemble [fe-s] clusters (i.e., the iscrsua-hscba-fdx operon) compensate for the lack of apbc during growth of an apbc strain on tcb. apbc had been linked to [fe-s] clu ... | 2009 | 19136587 |
| the proteome of salmonella typhimurium grown under in vivo-mimicking conditions. | to successfully infect a host, it is a prerequisite for enteric pathogens such as salmonella enterica serovar typhimurium to adapt to their environment, in casu the gastrointestinal tract. the adoption of an appropriate lifestyle is triggered by environmental signals such as the low oxygen availability and high osmolarity prevalent in the gut. in order to gain more insight in the changes that are induced when s. typhimurium is adapting to these particular conditions, we used 2-d dige technology ... | 2009 | 19137547 |
| campylobacter jejuni drives myd88-independent interleukin-6 secretion via toll-like receptor 2. | gastrointestinal disease caused by campylobacter jejuni is characterized by localized inflammation and the destruction of the epithelial cell barrier that forms host innate protection against pathogens. this can lead to an imbalance in fluid transport across the gastrointestinal tract, resulting in severe diarrhea. the mechanisms of host cell receptor recognition of c. jejuni and downstream immune signaling pathways leading to this inflammatory disease, however, remain unclear. the aim of this s ... | 2009 | 19139198 |
| recovery of salmonella from bermudagrass exposed to simulated wastewater. | most confined swine (sus scrofa) feeding operations in the southeastern united states hold manure in lagoons and apply effluent on bermudagrass [cynodon dactylon (l.) pers.] as fertilizer. salmonella enterica subsp. enterica (ex kauffman and edwards) le minor and popoff, has been reported in mississippi lagoons, but levels and potential for contamination of bermudagrass were unknown. a laboratory method was developed to examine salmonella contamination of bermudagrass and levels of salmonella we ... | 2009 | 19141824 |
| [report on typhoid fever case in maringá, state of paraná]. | typhoid fever is an acute bacterial disease caused by salmonella enterica serotype typhi, which is acquired by consumption of contaminated food or water. this paper had the aim of describing a case of typhoid fever that occurred in maringá, state of paraná, after three years without any notifications of the disease. | 2008 | 19142452 |
| transmission of salmonella between wildlife and meat-production animals in denmark. | to investigate the transmission of salmonella spp. between production animals (pigs and cattle) and wildlife on production animal farms in denmark. | 2008 | 19146492 |
| effects of repeated cycles of acid challenge and growth on the phenotype and virulence of salmonella enterica. | the aim of the study was to investigate how stresses like low ph, which may be encountered in farms or food preparation premises, shape populations of salmonella enterica by the selection of stress-resistant variants. | 2008 | 19146498 |
| typhoid rhabdomyolysis with acute renal failure and acute pancreatitis: a case report and review of the literature. | we report a case of typhoid rhabdomyolysis with acute renal failure and acute pancreatitis in a 23-year-old vietnamese male who was admitted to the intensive care unit with a 15-day history of fever followed by severe abdominal pain. on examination, the patient was febrile and his abdomen was diffusely tender. serum creatinine was 533 micromol/l, pancreatic amylase 1800 u/l and lipase 900 u/l; the myoglobin blood level was high, which is associated with significant myoglobinuria. blood, urine an ... | 2009 | 19147385 |
| rapid real-time pcr assay for detecting salmonella in raw and ready-to-eat meats. | a real-time pcr assay was evaluated for the rapid detection (10 h) of salmonella in meats using molecular beacon probes available as a commercial kit (iq-check, bio-rad laboratories). raw (chicken, pork) and ready-to-eat (rte) meats were artificially contaminated with salmonella enterica serovar typhimurium at the estimated level of 2 to 4 cells per 25 g. after 8 h of pre-enrichment in buffered peptone water, a molecular beacon-based pcr assay was performed to detect contamination in raw and rte ... | 2008 | 19149100 |
| comparative structural and molecular characterization of ribitol-5-phosphate-containing streptococcus oralis coaggregation receptor polysaccharides. | the antigenically related coaggregation receptor polysaccharides (rps) of streptococcus oralis strains c104 and sk144 mediate recognition of these bacteria by other members of the dental plaque biofilm community. in the present study, the structure of strain sk144 rps was established by high resolution nmr spectroscopy as [6galfbeta1-6galnacbeta1-3galalpha1-2ribitol-5-po(4)(-)-6galfbeta1-3galbeta1](n), thereby indicating that this polysaccharide and the previously characterized rps of strain c10 ... | 2009 | 19151140 |
| rapid oligonucleotide-based recombineering of the chromosome of salmonella enterica. | recombinant engineering using red recombinase-based approaches offers efficient and rapid approaches to deletion and modification of genes. here we describe a novel application of red recombinant engineering that enables direct manipulation of chromosomal loci by electroporation with short synthetic dna molecules. we demonstrate the use of this approach for the generation of scarless in-frame deletions in chromosomal genes of salmonella enterica. furthermore, we describe rapid site-directed muta ... | 2009 | 19151186 |
| evaluation of the bacterial diversity in cecal contents of laying hens fed various molting diets by using bacterial tag-encoded flx amplicon pyrosequencing. | laying hens are typically induced to molt to begin a new egg-laying cycle by withdrawing feed for up to 12 to 14 d. fasted hens are more susceptible to colonization and tissue invasion by salmonella enterica serovar enteritidis. much of this increased incidence in fasted hens is thought to be due to changes in the native intestinal microflora. an alternative to feed withdrawal involves feeding alfalfa meal crumble to hens, which is indigestible by poultry but provides fermentable substrate to th ... | 2009 | 19151343 |
| studying the mechanism of rna separations using rna chromatography and its application in the analysis of ribosomal rna and rna:rna interactions. | dna/rna chromatography presents a versatile platform for the analysis of nucleic acids. although the mechanism of separation of double stranded (ds) dna fragments is largely understood, the mechanism by which rna is separated appears more complicated. to further understand the separation mechanisms of rna using ion pair reverse phase liquid chromatography, we have analysed a number of dsrna and single stranded (ss) rna fragments. the high-resolution separation of dsrna was observed, in a similar ... | 2009 | 19155018 |
| rapid dna amplification using a battery-powered thin-film resistive thermocycler. | a prototype handheld, compact, rapid thermocycler was developed for multiplex analysis of nucleic acids in an inexpensive, portable configuration. instead of the commonly used peltier heating/cooling element, electric thin-film resistive heater and a miniature fan enable rapid heating and cooling of glass capillaries leading to a simple, low-cost thin-film resistive thermocycler (tfrt). computer-based pulse width modulation control yields heating rates of 6-7 k/s and cooling rates of 5 k/s. the ... | 2009 | 19159110 |
| pseudogene accumulation in the evolutionary histories of salmonella enterica serovars paratyphi a and typhi. | of the > 2000 serovars of salmonella enterica subspecies i, most cause self-limiting gastrointestinal disease in a wide range of mammalian hosts. however, s. enterica serovars typhi and paratyphi a are restricted to the human host and cause the similar systemic diseases typhoid and paratyphoid fever. genome sequence similarity between paratyphi a and typhi has been attributed to convergent evolution via relatively recent recombination of a quarter of their genomes. the accumulation of pseudogene ... | 2009 | 19159446 |
| the salmonella pathogenicity island 2-encoded type iii secretion system is essential for the survival of salmonella enterica serovar typhimurium in free-living amoebae. | free-living amoebae represent a potential reservoir and predator of salmonella enterica. through the use of type iii secretion system (t3ss) mutants and analysis of transcription of selected t3ss genes, we demonstrated that the salmonella pathogenicity island 2 is highly induced during s. enterica serovar typhimurium infection of acanthamoeba polyphaga and is essential for survival within amoebae. | 2009 | 19168655 |
| salmonella enterica serovar typhimurium mutants unable to convert malate to pyruvate and oxaloacetate are avirulent and immunogenic in balb/c mice. | previously, we showed that the salmonella enterica serovar typhimurium sr-11 tricarboxylic acid (tca) cycle must operate as a complete cycle for full virulence after oral infection of balb/c mice (m. tchawa yimga, m. p. leatham, j. h. allen, d. c. laux, t. conway, and p. s. cohen, infect. immun. 74:1130-1140, 2006). in the same study, we showed that for full virulence, malate must be converted to both oxaloacetate and pyruvate. moreover, it was recently demonstrated that blocking conversion of s ... | 2009 | 19168732 |
| sequence analysis of the plasmid pgy1 harbored in salmonella enterica serovar paratyphi a. | the cryptic plasmid pgy1, which is harbored by a clinical isolate of salmonella enterica serovar paratyphi a, was identified in a 9-year-old girl with paratyphoid fever in 2005, and its dna sequence was determined. it is 3592 bp in length and had a g+c content of 43.3%. three orfs were predicted that share low similarity with hypothetical proteins in the genbank database. pgy1 shared 36.6% sequence homology with the cryptic plasmid pimvs1 from salmonella typhimurium. its unique sequence makes it ... | 2009 | 19169860 |
| acid stress activation of the sigma(e) stress response in salmonella enterica serovar typhimurium. | the alternative sigma factor sigma(e) is activated by unfolded outer membrane proteins (omps) and plays an essential role in salmonella pathogenesis. the canonical pathway of sigma(e) activation in response to envelope stress involves sequential proteolysis of the anti-sigma factor rsea by the pdz proteases degs and rsep. here we show that sigma(e) in salmonella enterica sv. typhimurium can also be activated by acid stress. a sigma(e)-deficient mutant exhibits increased susceptibility to acid ph ... | 2009 | 19170886 |
| isolation of salmonella enterica serotype isangi from a suspected case of enteric encephalopathy. | nontyphoidal salmonella species are thought to be potentially infectious to humans and many are documented to cause human diseases. we isolated s. isangi from the blood of a 30-year-old man with complaints of diarrhoea, fever, and altered sensorium. the serotype of the isolate was confirmed at national salmonella centre (vet.), division of bacteriology and mycology, indian veterinary research institute, izzatnagar, india. the isolate was not an extended spectrum beta-lactamase (esbl) producer an ... | 2009 | 19172065 |
| [mechanism of interaction of tumor necrosis factor (tnf-alpha) of macroorganism with the salmonella enterica cells (ser. typhimurium)]. | the goal of this work was to elucidate the mechanism of direct interaction of bacterial cells with tumor necrosis factor (tnf-alpha; cytokine). it was shown earlier that this interaction facilitated activation of bacterial growth and recultivation of non-cultivated forms in vitro and in vivo. it was shown in experiments with mice deficient in the genes encoding eucaryotic tnf-alpha receptors and infected with salmonella that addition of exogenous tnf-alpha to suspension of infection cells caused ... | 2008 | 19172874 |
| prevalence and risk factors for bacterial food-borne zoonotic hazards in slaughter pigs: a review. | the hygiene package and regulation ec-2160/2003 require information flow from farm to slaughterhouse to enhance european consumers protection in a 'farm to fork' approach. this obligation especially concerns food-borne zoonotic hazards transmitted to humans through pork consumption, such as thermophilic campylobacter spp., listeria monocytogenes, salmonella enterica and yersinia enterocolitica. prevalence estimates of these four hazards are affected by the sampling strategy and diagnostic proced ... | 2009 | 19175574 |
| occurrence of extended-spectrum beta-lactamase-producing salmonella enterica in northern spain with evidence of ctx-m-9 clonal spread among animals and humans. | among the 1233 salmonella enterica isolates obtained in two spanish hospitals, five isolates (0.4%) (serovars: virchow, four; livingstone, one) had the phenotype of an extended-spectrum beta-lactamase (esbl) producer. the genetic characterization of the esbl of s. enterica livingstone revealed a bla(shv-2) gene. the bla(ctx-m-10) gene in a phage-related genetic environment was found in one s. enterica virchow isolate, and the bla(ctx-m-9) gene within the in60 integron was found in the three rema ... | 2009 | 19175621 |
| [application of pulsed-field gel electrophoresis in a food-borne outbreak of salmonella serotype muenchen infection]. | to investigate the application of pulsed-field gel electrophoresis (pfge) in food-borne outbreak. | 2008 | 19176144 |
| anthrax protective antigen delivered by salmonella enterica serovar typhi ty21a protects mice from a lethal anthrax spore challenge. | bacillus anthracis, the etiological agent of anthrax disease, is a proven weapon of bioterrorism. currently, the only licensed vaccine against anthrax in the united states is ava biothrax, which, although efficacious, suffers from several limitations. this vaccine requires six injectable doses over 18 months to stimulate protective immunity, requires a cold chain for storage, and in many cases has been associated with adverse effects. in this study, we modified the b. anthracis protective antige ... | 2009 | 19179420 |
| effect of hemidesmus indicus r.br. root extract against salmonella enterica serovar typhimurium-induced apoptosis in murine macrophage cell line (p388d1). | previous studies on natural products had mainly dealt with their antimicrobial activity and studies on the interference of these bioactive compounds with host-bacterial interaction is limited. the present study was undertaken to investigate the effect of the sterols and fatty acids present in the chloroform fraction of crude methanol extract of hemidesmus indicus root (chi) on salmonella enterica serovar typhimurium (s. typhimurium) mediated apoptosis in a murine macrophage cell line (p388d1). | 2008 | 19179687 |
| inc a/c plasmids are prevalent in multidrug-resistant salmonella enterica isolates. | bacterial plasmids are fragments of extrachromosomal double-stranded dna that can contain a variety of genes that are beneficial to the host organism, like those responsible for antimicrobial resistance. the objective of this study was to characterize a collection of 437 salmonella enterica isolates from different animal sources for their antimicrobial resistance phenotypes and plasmid replicon types and, in some cases, by pulsed-field gel electrophoresis (pfge) in an effort to learn more about ... | 2009 | 19181840 |
| salmonella enterica serotype typhimurium std fimbriae bind terminal alpha(1,2)fucose residues in the cecal mucosa. | the std operon encodes a fimbrial adhesin of salmonella enterica serotype typhimurium that is required for attachment to intestinal epithelial cells and for cecal colonization in the mouse. to study the mechanism by which this virulence factor contributes to colonization we characterized its binding specificity. std-mediated binding to human colonic epithelial (caco-2) cells could be abrogated by removing n-linked glycans. adherence of std fimbriated s. typhimurium to caco-2 cells could be block ... | 2009 | 19183274 |
| protection against experimental fowl typhoid by parenteral administration of live sl5828, an aroa-serc (aromatic dependent) mutant of a wild-type salmonella gallinarum strain made lysogenic for p22 sie. | a wild-type strain of salmonella enterica serotype gallinarum, lysogenized with p22 sie (superinfection-exclusion defective) was greatly attenuated for newly hatched or 21-day-old chickens. an aroa transductant of the lysogenic strain and an aroa-serc tetracycline-sensitive deletion or deletioninversion mutant of the latter were equally attenuated. intramuscular administration of the aroa-serc strain to 21-day-old chickens protected them against oral challenge with 10(6) colony forming units of ... | 2000 | 19184834 |
| evaluation of the effect of mannan-oligosaccharides on the competitive exclusion of salmonella enteritidis colonization in broiler chicks. | this study evaluated the protection against colonization by salmonella enterica serovar enteritidis in the chick's caecum when dosed with caecal contents from hens fed dietary carbohydrates based on mannose. protection was investigated by comparing the effects of four suspensions of hen caecal contents (hcc) fed diets supplemented with 2.5% d -mannose (man-hcc), 2.5% mannoseoligosaccharide (mos-hcc), 2.5% palm kernel meal (pkm-hcc) or unsupplemented mash (mashhcc). four trials to quantify and co ... | 2000 | 19184854 |
| pathogenicity of salmonella enteritidis phage types 4, 8 and 23 in specific pathogen free chicks. | the pathogenicity of two isolates of salmonella enterica serovar enteritidis (se) phage type (pt) 4, three of pt8 and one of pt23 was investigated in groups of 1-day-old specific pathogen free white leghorn chicks. two groups were crop gavaged with each culture but at two different doses. two additional groups were given salmonella enterica serovar pullorum (sp) at similar doses and one further group served as uninoculated controls. body weights were recorded at 14, 21, and 28 days postinoculati ... | 2000 | 19184855 |
| experimental salmonella enterica serovar pullorum infection in two commercial varieties of laying hens. | an experiment was carried out to investigate the biology of salmonella pullorum in two varieties of laying hens, from 5 days of age up to 9 months. one variety was resistant to systemic salmonellosis (light layers producing white eggs) and the other was considered susceptible (brown layers producing brown eggs). the brown birds were more affected by the infection, showing signs of clinical disease in the first month of life. later, these signs disappeared, but postmortem examination revealed per ... | 2001 | 19184886 |
| observations on the persistence and vertical transmission of salmonella enterica serovars pullorum and gallinarum in chickens: effect of bacterial and host genetic background. | commercial laying hens inoculated with a strain of salmonella enterica ser. pullorum when they were 4 days old showed no morbidity, but harboured infection until they came into lay, and then produced s. pullorum-contaminated eggs and infected progeny. there was limited evidence of transmission of maternal immunity to the progeny. attempts were made to set up similar infections in hens with salmonella gallinarum, but without success. infection either resulted in clinical disease or elimination of ... | 2001 | 19184904 |
| further studies on vertical transmission and persistence of salmonella enterica serovar enteritidis phage type 4 in chickens. | one-week-old commercial layers were infected orally with 10(8) colony forming units of salmonella enterica serovar enteritidis phage type 4. no mortality was observed. the inoculated organism was isolated in decreasing viable numbers from a number of tissues, particularly the spleen, liver and caeca. organisms present in the spleen were primarily localized within macrophages. no salmonella enteritidis organisms were isolated between 10 and 24 weeks of age, when the experiment was terminated afte ... | 2001 | 19184915 |
| importance of subunit vaccine antigen of major fli c antigenic site of salmonella enteritidis ii: a challenge trial. | salmonella enterica subsp. enterica serovar enteritidis (se) infection in chickens shows a mild pathogenicity except for young ages, compared with other animals, and laying hens sometimes produce se-contaminated eggs leading to public health concerns. to reduce the problem, se bacterin in poultry farms has been applied. we previously demonstrated that a subunit antigen, g.m. part polypeptide in se-fli c (sep 9), could be a candidate subunit antigen of se vaccine which may show less side effects ... | 2009 | 19186198 |
| the cysteine 354 and 277 residues of salmonella enterica serovar typhi envz are determinants of autophosphorylation and ompr phosphorylation. | an initial biochemical characterization of the salmonella enterica serovar typhi (s. typhi) envz sensor protein and several mutant derivatives was performed. autophosphorylation levels were higher for escherichia coli envz, intermediate for s. enterica serovar typhimurium envz and very low for s. typhi envz, in spite of their high amino acid sequence identity. consequently, ompr phosphorylation was related to envz autophosphorylation. among the mutant derivatives, a c354g mutation in s. typhi en ... | 2009 | 19187206 |
| synergistic role of curli and cellulose in cell adherence and biofilm formation of attaching and effacing escherichia coli and identification of fis as a negative regulator of curli. | curli are adhesive fimbriae of escherichia coli and salmonella enterica. expression of curli (csga) and cellulose (bcsa) is co-activated by the transcriptional activator csgd. in this study, we investigated the contribution of curli and cellulose to the adhesive properties of enterohaemorragic (ehec) o157:h7 and enteropathogenic e. coli (epec) o127:h6. while single mutations in csga, csgd or bcsa in epec and ehec had no dramatic effect on cell adherence, double csgabcsa mutants were significantl ... | 2009 | 19187284 |
| serovar distribution and antimicrobial susceptibility of swine salmonella isolates from clinically ill pigs in diagnostic submissions from indiana in the united states. | to determine serovar distribution and levels of antimicrobial susceptibility of salmonella isolated from clinically ill pigs in diagnostic submissions. | 2009 | 19187513 |
| recombinant attenuated salmonella harboring 4-1bb ligand gene enhances cellular immunity. | to transfect antigen presenting cells (apcs) with 4-1bb ligand dna by attenuated salmonella enterica serovar typhimurium in vivo, and to observe the effects of ectogenous 4-1bbl on the immune functions of infected rats. | 2009 | 19187795 |
| rethinking typhoid fever vaccines: implications for travelers and people living in highly endemic areas. | 2009 | 19192128 | |
| effects of the dicarboxylic acids malate and fumarate on e. coli o157:h7 and salmonella enterica typhimurium populations in pure culture and in mixed ruminal microorganism fermentations. | the dicarboxylic acids malate and fumarate increase ruminal ph, reduce methane production, increase propionate and total volatile fatty acid (vfa) production, and reduce lactic acid accumulation in a manner similar to ionophores. these acids stimulate the ruminal bacterium selenomonas ruminantium to ferment lactate to produce propionate. thus, dicarboxylic acids have been suggested as nonantibiotic modifiers of the ruminal fermentation, but their impact on ruminal microbial ecology remains unkno ... | 2009 | 19194750 |