Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| new multilocus genotypes of giardia lamblia human isolates. | giardia lamblia is considered a species complex, whose members show little differences in their morphology, but have remarkable genetic variability. the aim of this study was to identify inter- and intra-assemblage genetic variation in g. lamblia among patients in rio de janeiro. the parasitological study was performed on faeces, and dna was extracted from the samples which tested positive for g. lamblia. the genetic assemblages and subtypes were determined via multilocus sequence typing (mlst) ... | 2017 | 28669825 |
| recreational waterborne illnesses: recognition, treatment, and prevention. | illness after recreational water activities can be caused by a variety of agents, including bacteria, viruses, parasites, algae, and even chlorine gas. these illnesses are more common in summer. waterborne illnesses are underreported because most recreational activity occurs in unsupervised venues or on private property, and participants tend to disperse before illness occurs. symptoms of waterborne illness are primarily gastrointestinal, but upper respiratory and skin manifestations also occur. ... | 2017 | 28671392 |
| acute acalculous cholecystitis caused by giardia lamblia. | we report a case of a 70-year-old man with acute acalculous cholecystitis caused by giardia lamblia. contrast-enhanced computed tomography (ct) showed distention of the gallbladder due to a pericholecystic abscess without gallstones. magnetic resonance cholangiopancreatography and drip infusion cholecystocholangiography-ct demonstrated a stricture of the hilar bile duct and cystic duct obstruction. we conducted transpapillary bile duct brush cytology and a biopsy of the hilar bile duct stricture ... | 2017 | 28674353 |
| myosin-independent cytokinesis in giardia utilizes flagella to coordinate force generation and direct membrane trafficking. | devoid of all known canonical actin-binding proteins, the prevalent parasite giardia lamblia uses an alternative mechanism for cytokinesis. unique aspects of this mechanism can potentially be leveraged for therapeutic development. here, live-cell imaging methods were developed for giardia to establish division kinetics and the core division machinery. surprisingly, giardia cytokinesis occurred with a median time that is ∼60 times faster than mammalian cells. in contrast to cells that use a contr ... | 2017 | 28679631 |
| morphological and physiological characteristics of a virulent and zoonotic assemblage a giardia duodenalis canine strain. | giardiasis is an intestinal parasitosis that affects millions of people worldwide and is considered a zoonotic disease. frequently in contact with humans, dogs are the main host involved in this zoonotic transmission. here, we compared some aspects of giardia duodenalis biology between two strains: a recently isolated dog strain (bhfc1) and a human reference strain (portland-1). growth curve analysis revealed that bhfc1 trophozoites multiply faster than the human isolate portland-1 in axenic cul ... | 2017 | 28689000 |
| sorting without a golgi complex. | the endoplasmic reticulum-golgi-target organelle route is one of the most studied events and has fascinated researchers for years. however, the conservative mechanism of protein sorting and delivery is now being challenged by the finding of unconventional pathways driving protein sorting and transport. protozoa parasites are being rediscovered as good models for analyzing alternative targeting pathways, associated with their ability to adapt to diverse environments and hosts. here, we have gathe ... | 2017 | 28696551 |
| endoplasmic reticulum is the sorting core facility in the golgi-lacking protozoan giardia lamblia. | our understanding of protein and lipid trafficking in eukaryotic cells has been challenged by the finding of different forms of compartmentalization and cargo processing in protozoan parasites. here, we show that, in the absence of a golgi compartment in giardia, proteins destined for secretion are directly sorted and packaged at specialized er regions enriched in copii coatomer complexes and ceramide. we also demonstrated that er-resident proteins are retained at the er by the action of a kdel ... | 2017 | 28696565 |
| reduced efficacy of albendazole against ascaris lumbricoides in rwandan schoolchildren. | control of human soil-transmitted helminths (sths) relies on preventive chemotherapy of schoolchildren applying the benzimidazoles (bz) albendazole or mebendazole. anthelmintic resistance (ar) is a common problem in nematodes of veterinary importance but for human sths, information on drug efficacy is limited and routine monitoring is rarely implemented. herein, the efficacy of single dose albendazole (400 mg) was evaluated in 12 schools in the huye district of rwanda where ascaris is the predom ... | 2017 | 28697451 |
| detection of zoonotic and livestock-specific assemblages of giardia duodenalis in free-living wild lizards. | giardia duodenalis is a zoonotic parasite that infects the gut of a wide range of vertebrates, including numerous wildlife species. however, little is known about this protozoan parasite in reptiles. fecal samples from 31 wild lizards were collected in galicia (northwest spain) and screened for the presence of giardia by pcr amplification and sequencing of the its1-5.8s-its2 region in the ribosomal unit. this allowed detection of the parasite in 5 samples (16.1%), and enabled identification of g ... | 2017 | 28699999 |
| intestinal parasitic infections: current status and associated risk factors among school aged children in an archetypal african urban slum in nigeria. | intestinal parasitic infections (ipis) among school aged children (sac) in nigeria remains endemic, hence the need for regular surveillance to attract the attention of policy makers. this cross-sectional study investigated the current prevalence and factors associated with intestinal parasitic infections among school aged children in an urban slum of lagos city, nigeria. | 2017 | 28711437 |
| prevalence of enteric infections among hospitalized patients in two referral hospitals in ghana. | diarrhea is an important cause of morbidity and mortality worldwide. in africa and ghana in particular, it is estimated to contribute directly to 19 and 25% of pediatric mortality among children under 5 years, respectively. | 2017 | 28716138 |
| stool antigen immunodetection for diagnosis of giardia duodenalis infection in human subjects with hiv and cancer. | human infection with the protozoan parasite giardia duodenalis is one the most common parasitic diseases worldwide. higher incidence rates of giardiasis have been reported from human subjects with multiple debilitating chronic conditions, including hypogammaglobulinemia and common variable immunodeficiency (cvid). in the current study, stool specimens were collected from 199 individuals diagnosed with hiv or cancer and immunocompetent subjects. the sensitivity of microscopy-based detection on fr ... | 2017 | 28716658 |
| comparison of cytokine responses in ecuadorian children infected with giardia, ascaris, or both parasites. | abstractmore than 2 billion people are infected with parasites globally, and the majority have coinfections. intestinal protozoa and helminths induce polarizing cd4(+) t-helper cell 1 (th1) mediated cytokine responses within the host. such immune polarization may inhibit the ability of the host to mount an adequate immune response for pathogen clearance to concurrent pathogens. the current study evaluated the plasma cytokine profile in ascaris and giardia coinfected children compared with giardi ... | 2017 | 28719267 |
| cryptosporidium infection in children with cancer undergoing chemotherapy: how important is the prevention of opportunistic parasitic infections in patients with malignancies? | cryptosporidiosis is a relatively uncommon disease in healthy individuals but could be potentially worrisome in immunocompromised patients. this study aimed to evaluate cryptosporidium infection in children with cancer undergoing chemotherapy. a case-control study was conducted in 132 children with cancer undergoing chemotherapy and 132 non-cancer controls. the modified ziehl-neelsen (mzn) staining and polymerase chain reaction methods were used for the detection of cryptosporidium parasite. all ... | 2017 | 28730516 |
| cryptosporidium and giardia infections in children: a review. | diarrheal disease remains the second leading cause of mortality in children in developing countries. cryptosporidium is a leading cause and its importance stands to increase as rotavirus vaccine becomes used around the world. cryptosporidium is particularly problematic in children younger than 2 years old and in the immunocompromised. giardia lamblia is a common intestinal protozoan that is associated with diarrhea and, perhaps, growth faltering in impoverished settings. this review establishes ... | 2017 | 28734513 |
| use of serologic responses against enteropathogens to assess the impact of a point-of-use water filter: a randomized controlled trial in western province, rwanda. | diarrhea is a leading contributor to childhood morbidity and mortality in sub-saharan africa. given the challenge of blinding most water, sanitation, and hygiene (wash) interventions, diarrheal disease outcome measures in wash intervention trials are subject to potential bias and misclassification. using the platform of a cluster-randomized controlled trial of a household-based drinking water filter in western province, rwanda, we assessed the impact of the drinking water filter on enteric seroc ... | 2017 | 28749764 |
| cross-modulation of pathogen-specific pathways enhances malnutrition during enteric co-infection with giardia lamblia and enteroaggregative escherichia coli. | diverse enteropathogen exposures associate with childhood malnutrition. to elucidate mechanistic pathways whereby enteric microbes interact during malnutrition, we used protein deficiency in mice to develop a new model of co-enteropathogen enteropathy. focusing on common enteropathogens in malnourished children, giardia lamblia and enteroaggregative escherichia coli (eaec), we provide new insights into intersecting pathogen-specific mechanisms that enhance malnutrition. we show for the first tim ... | 2017 | 28750066 |
| immunologic detection of giardia duodenalis in a specific pathogen-free captive olive baboon ( papio cynocephalus anubis) colony. | several commercial giardia immunoassays were evaluated in baboons for sensitivity and specificity as well as ease of use in a large specific pathogen-free (spf) colony. an additional objective was to identify the assemblage(s) of giardia duodenalis present in this baboon colony. a direct immunofluorescent antibody test (ifat) was used as the reference test. tests evaluated were a patient-side rapid test for dogs and cats, a human rapid test, and a well-plate elisa designed for use with humans. t ... | 2017 | 28754076 |
| genetic variation of giardia lamblia isolates from food-handlers in kashan, central iran. | based on genotyping study of human isolates of giardia lamblia; humans are mainly infected by two assemblages a and b. the present study was carried out to determine the sub-assemblages of g. lamblia isolated from food handlers referred to kashan health centers, central iran, 2015. | 2017 | 28761464 |
| detection and multilocus genotyping of giardia duodenalis in dogs in sichuan province, china. | giardia duodenalis (also known as g. intestinalis) is a flagellated protozoan that parasitizes the small intestine and is a common causal agent of zoonotic infections in humans and animals. to assess the genetic diversity and zoonotic transmission potential of g. duodenalis in stray dogs, 159 fecal specimens were collected from dogs in chengdu, yaan, and leshan in sichuan province, china. of the 159 fecal samples from stray dogs, 18 (11.3%) were g. duodenalis-positive based on nested pcr amplifi ... | 2017 | 28770713 |
| insights into the giardia intestinalis enolase and human plasminogen interaction. | giardia intestinalis is an intestinal parasite that causes diarrhea in humans and animals worldwide. the enolase of g. intestinalis (gieno) participates in its glycolysis pathway and is abundantly expressed in the parasite cytosol; however, its localization on the surface of trophozoites and cysts has been demonstrated. enolases from bacteria and parasites can have different functions and are considered moonlighting proteins, for example, as a cell surface plasminogen receptor. in relation to gi ... | 2017 | 28770921 |
| a method for incorporating a time-dose-response model into a giardia lamblia outbreak. | experimental time-to-infection data is a useful, but often underutilized, material for examining the mechanics of in vivo pathogen growth. in this paper, the authors attempt to incorporate a time-dose-response (tdr) equation into a model which predicts the number of ill persons per day in a giardia lamblia epidemic using data collected from a pittsfield, massachusetts outbreak. to this end, dose-response and tdr models were generated for giardia exposure to beaver and human volunteers, and a max ... | 2017 | 28771146 |
| evaluation of the bd max enteric parasite panel for the detection of cryptosporidium parvum/hominis, giardia duodenalis and entamoeba histolytica. | conventional laboratory detection methods for gastrointestinal parasites are time consuming, require considerable technical expertise and may suffer from poor analytical sensitivity. this study sought to evaluate the automated bd max enteric parasite panel (epp) for the detection of cryptosporidium parvum/hominis, entamoeba histolytica and giardia duodenalis.methodolgy. a total of 104 known positive samples (43 cryptosporidium parvum/hominis and 61 g. duodenalis), 15 simulated samples (e. histol ... | 2017 | 28792380 |
| socioenvironmental conditions and intestinal parasitic infections in brazilian urban slums: a cross-sectional study. | a cross-sectional study covering an agglomeration of urban slums was conducted between 2015 and 2016 using participants observation, a socioeconomic survey, and the spontaneous sedimentation method with three slides per sample to analyze fresh stool specimens ( n =595) searching for intestinal parasites. | 2017 | 28793024 |
| environmental aspects related to tuberculosis and intestinal parasites in a low-income community of the brazilian amazon. | we carried out a cross-sectional study from january to december 2015 on 1,425 inhabitants from a floating population in the brazilian amazon (murinin district, pará state) to describe the population-based prevalence of tuberculosis (tb) from 2011 to 2014, recent tb contacts (rcts) latently infected with mycobacterium tuberculosis (ltbi) , the coverage of the local health network, socio-environmental factors, and frequency of intestinal parasitic infection (ipi). we found that the sanitary struct ... | 2017 | 28793025 |
| larrea tridentata: a novel source for anti-parasitic agents active against entamoeba histolytica, giardia lamblia and naegleria fowleri. | protozoan parasites infect and kill millions of people worldwide every year, particularly in developing countries where access to clean fresh water is limited. among the most common are intestinal parasites, including giardia lamblia and entamoeba histolytica. these parasites wreak havoc on the epithelium lining the small intestines (g. lamblia) and colon (e. histolytica) causing giardiasis and amebiasis, respectively. in addition, there are less common but far more deadly pathogens such as naeg ... | 2017 | 28793307 |
| variation in basal body localisation and targeting of trypanosome rp2 and for20 proteins. | tof-lish-pll motifs define fop family proteins; some members are involved in flagellum assembly. the critical role of fop family protein for20 is poorly understood. here, we report relative localisations of the four fop family proteins in parasitic trypanosoma brucei: tbrp2, tbofd1 and tbfop/fop1-like are mature basal body proteins whereas tbfor20 is present on pro- and mature basal bodies - on the latter it localises distal to tbrp2. we discuss how the data, together with published work for ano ... | 2017 | 28822909 |
| cysteine protease-dependent mucus disruptions and differential mucin gene expression in giardia duodenalis infection. | the intestinal mucus layer provides a critical host defense against pathogen exposure and epithelial injury, and yet little is known of how enteropathogens may circumvent this physiological barrier. giardia duodenalis is a small intestinal parasite responsible for diarrheal disease and chronic post-infectious illness. this study reveals a complex interaction at the surface of epithelial cells, between g. duodenalis and the intestinal mucus layer. here, we reveal mechanisms whereby g. duodenalis ... | 2017 | 28823873 |
| molecular analysis of the enteric protozoa associated with acute diarrhea in hospitalized children. | pediatric diarrhea is a common cause of death among children under 5 years of age. in the current study, we investigated the frequency of intestinal parasites among 580 pediatric patients with chronic diarrhea. parasitic protozoa (all species combined) were detected by molecular tools in 22.9% of the children and the most common parasite was cryptosporidium spp. (15.1%). blastocystis hominis was detected in 4.7%, dientamoeba fragilis in 4%, giardia duodenalis in 1.7%, and entamoeba histolytica i ... | 2017 | 28824878 |
| the intersection of immune responses, microbiota, and pathogenesis in giardiasis. | giardia lamblia is one of the most common infectious protozoans in the world. giardia rarely causes severe life-threatening diarrhea, and may even have a slight protective effect in this regard, but it is a major contributor to malnutrition and growth faltering in children in the developing world. giardia infection also appears to be a significant risk factor for postinfectious irritable bowel and chronic fatigue syndromes. in this review we highlight recent work focused on the impact of giardia ... | 2017 | 28830665 |
| survey of the occurrence and human infective potential of giardia duodenalis and cryptosporidium spp. in wastewater and different surface water sources of western romania. | from the group of parasitic protozoa, giardia and cryptosporidium are the most common pathogens spread in surface water sources, representing a continuous threat to public health and water authorities. the aim of this survey was to assess the occurrence and human infective potential of these pathogens in treated wastewaters and different surface water sources. a total of 76 western romanian water bodies in four counties (arad, bihor, caraș-severin and timiș) were investigated, including the effl ... | 2017 | 28832257 |
| evaluation of primitive ground water supplies as a risk factor for the development of major waterborne zoonosis in egyptian children living in rural areas. | endemic waterborne zoonosis frequently occurs in both developed and less developed countries. thus, bio-surveillance of waterborne zoonosis is a "necessity" for the implementation of effective preventive public health measures in egyptian rural areas. the primitive individual water supplies created by the rural agriculture population, primarily from ground water, usually maximize the customers' exposure to impurity pathogens via diffused humans and animal excreta or wastages. the current study a ... | 2017 | 28843417 |
| intestinal parasitic infections: current prevalence and risk factors among schoolchildren in capital area of the republic of marshall islands. | intestinal parasitic infections (ipis) among schoolchildren in republic of marshall islands (rmi) largely remains unknown, thus investigation on ipis status to establish the baseline data is urgently needed. this cross-sectional study intended to investigate the current ipis status and associated risk factors among schoolchildren at capital of rmi. single stool sample from 400 schoolchildren (207 boys and 193 girls) aged 9.73±2.50 yrs old was examined by employing merthiolate-iodine-formaldehyde ... | 2017 | 28847672 |
| an overview of parasitic infections of the gastro-intestinal tract in developed countries affecting immunocompromised individuals. | in both developed and developing countries, parasitic infections continue to be a frequent cause of mortality and morbidity. due to the globalization of the world, doctors must be fully prepared to deal with a plethora of parasitic infections. more commonly the gastrointestinal (gi) tract is infected and in developed countries protozoans are more likely to be the cause of infection compared to helminths. these gi protozoa are progressively becoming recognized as important pathogens in patients t ... | 2017 | 28848249 |
| prevalence of intestinal parasitic infection among primary school children in southern iran. | a cross-sectional study was carried out to determine the prevalence of intestinal parasites in primary school children in bandar abbas, southern iran during january to march 2016. single stool samples were collected from 1465 participants by clean stool cup. the questionnaire was prepared on the basis of demographic characteristics. the diagnosis was made on the basis of the direct wet mount, formalin-ether concentration, and ziehl-neelsen and trichrome stained slides. out of the 1465 school chi ... | 2017 | 28848255 |
| antiprotozoal activity of extracts of elaeodendron trichotomum (celastraceae). | chagas disease, amebiasis, giardiasis and trichomoniasis represent a serious health problem in latin america. the drugs employed to treat these illnesses produce important side effects and resistant strains have appeared. the present study was aimed to evaluate the antiprotozoal activity of leaves, stem bark and root bark of elaeodendron trichotomum, a celastraceus, that is used in mexico as an anti-infective in febrile-type diseases. | 2016 | 28852732 |