Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| identification of an extracellular matrix protein adhesin, emaa, which mediates the adhesion of actinobacillus actinomycetemcomitans to collagen. | actinobacillus actinomycetemcomitans is an aetiologic agent in the development of periodontal and some systemic diseases in humans. this pathogen localizes to the underlying connective tissue of the oral cavity in individuals with periodontal disease. the adhesion of a. actinomycetemcomitans to extracellular matrix components of the connective tissue prompted this study to identify gene products mediating the interaction of a. actinomycetemcomitans to these molecules. a transposon mutagenesis sy ... | 2004 | 15289564 |
| complete genomic nucleotide sequence of the temperate bacteriophage aa phi 23 of actinobacillus actinomycetemcomitans. | the entire double-stranded dna genome of the actinobacillus actinomycetemcomitans bacteriophage aa phi 23 was sequenced. linear dna contained in the phage particles is circularly permuted and terminally redundant. therefore, the physical map of the phage genome is circular. its size is 43,033 bp with an overall molar g+c content of 42.5 mol%. sixty-six potential open reading frames (orfs) were identified, including an orf resulting from a translational frameshift. a putative function could be as ... | 2004 | 15292156 |
| implant therapy following liver transplantation: clinical and microbiological results after 10 years. | the number of immunocompromised patients is increasing as a consequence of successful organ transplantation. placing dental implants in these patients has been questioned because of their increased risk for infections. the 10-year follow-up data of a 71-year-old liver transplant recipient with long-term immunosuppressive therapy is reported. six months after liver transplantation, two interforaminal implants were inserted in the edentulous mandible, and an overdenture using non-rigid telescopic ... | 2004 | 15295960 |
| microbiological shifts in intra- and extraoral habitats following mechanical periodontal therapy. | the aim of the present study was to analyze the intra- and extraoral colonization dynamics of periodontal pathogens following supra- and subgingival debridement. | 2004 | 15312101 |
| dendritic cells stimulated with actinobacillus actinomycetemcomitans elicit rapid gamma interferon responses by natural killer cells. | human immunoglobulin g2 (igg2) responses are gamma interferon (ifn-gamma) dependent, and monocyte-derived dendritic cells (mdcs) promote igg2 production. dcs spontaneously emerge from monocytes in cultures prepared from localized aggressive periodontitis (lagp) patients, and these patients have high levels of igg2 that is reactive with actinobacillus actinomycetemcomitans. these results prompted the hypothesis that an interaction between mdcs and a. actinomycetemcomitans promotes ifn-gamma produ ... | 2004 | 15322002 |
| initial antimicrobial effect of controlled-release doxycycline in subgingival sites. | to determine the ability of a 10% doxycycline hyclate controlled-release polymer (atridox) to suppress periodontopathic bacteria when placed subgingivally following scaling and root planing (sc/rp). | 2004 | 15324352 |
| gamma-interferon enhances expression of cd14/myd88 and subsequent responsiveness to lipopolysaccharide from actinobacillus actinomycetemcomitans in human gingival fibroblasts. | cd14, toll-like receptor 4 (tlr4) and myd88 have been shown to mediate responsiveness in host cells to lipopolysaccharide. we investigated here the regulatory effects of inflammatory cytokines on the expression of membrane cd14 (mcd14), tlr4 and myd88, and on subsequent responsiveness to lipopolysaccharide from actinobacillus actinomycetemcomitans in human gingival fibroblasts. | 2004 | 15324355 |
| quantitative detection of periodontopathogens by real-time pcr. | specific bacteria are believed to play an important role in chronic periodontitis, yet the significance of their relative numbers in initiation and progress of the disease is still unclear. we report here the development of a sensitive, quantitative pcr technique for enumerating actinobacillus actinomycetemcomitans (aa), porphyromonas gingivalis (pg), prevotella intermedia (pi), dialister pneumosintes (dp) and micromonas micros (mm) as well as total eubacteria in subgingival plaque samples from ... | 2004 | 15325758 |
| microbiological profile of early onset/aggressive periodontitis patients. | the objectives of this study were to characterize the bacterial profile and to seek possible bacterial associations in the subgingival microbiota of early onset periodontitis/aggressive periodontitis patients by using two different techniques, culture and immunofluorescence. | 2004 | 15327644 |
| clonal distribution of natural competence in actinobacillus actinomycetemcomitans. | the competence for natural transformation was investigated in 67 actinobacillus actinomycetemcomitans strains. the transformation assays were performed with both cloned dna fragments and chromosomal markers of a. actinomycetemcomitans. competence was found in 12 of 18 serotype a strains, 0 of 21 serotype b strains, 0 of 14 serotype c strains, 3 of 6 serotype d strains, 3 of 4 serotype e strains, 0 of 3 serotype f strains, and 0 of 1 nonserotypeable strain. the transformation frequencies varied f ... | 2004 | 15327649 |
| dna probe analysis in smoker and non smoker rapidly progressive periodontitis patients--a pilot study. | smoking is one of the most significant risk factors in the development and further advancement of inflammatory periodontal disease. the bacteria a. actinomycetemcomitans, p. gingivalis and p. intermedius as indicated as the potential pathogens associated with periodontal disease. since the bacteria mentioned as well as smoking are factors associated with periodontitis it is of importance to elucidate the interrelationship between these factors. the purpose of this study was to investigate the pr ... | 2003 | 15328997 |
| relationship of subgingival and salivary microbiota to gingival overgrowth in heart transplant patients following cyclosporin a therapy. | severe gingival overgrowth (go) is induced in patients taking cyclosporin a (csa) following organ transplantation. determining which patient will develop go is still not possible. the purpose of this study was to establish an association between csa and gingival overgrowth in heart transplant patients taking into account periodontal and microbiological conditions. | 2004 | 15341348 |
| localized aggressive periodontitis in a six-year-old: a case report. | the purpose of this report was to describe an approach to diagnose and effectively treat a pediatric patient with localized aggressive periodontitis. a 6-year-old female presented with clinical and radiographic evidence of severe attachment loss around several primary teeth. she had no history of systemic disease, periodontal disease, or caries prior to the periodontal abscess that prompted her referral. routine immunological tests did not reveal any functional defects, but dna testing for perio ... | 2004 | 15344629 |
| clinical and microbiologic changes after removal of orthodontic appliances. | the goal of this study was to evaluate the clinical and microbiological factors associated with orthodontic appliances during an episode of gingival inflammation and the impact of appliance removal on periodontal health. this prospective study included 10 patients, aged 12 to 20 years, with clinical signs of gingival inflammation at the final phase of orthodontic treatment (appliance removal). plaque index, gingival index, and probing depth were evaluated, and microbiological samples were collec ... | 2004 | 15356501 |
| prevalence of periodontal pathogens in dental plaque of children. | porphyromonas gingivalis, actinobacillus actinomycetemcomitans, and tannerella forsythensis have been implicated as the main etiological agents of periodontal disease. the purpose of this work was to estimate the prevalence of these organisms in plaque from children without gingivitis (group 1; n = 65) and from those with gingivitis (group 2; n = 53). extracted dna from plaque was subjected to two rounds of pcr targeting the 16s rrna gene using both universal primers and species-specific primers ... | 2004 | 15365002 |
| considerations in evaluating the applicability of universal detection of oral pathogens. | 2004 | 15365062 | |
| card15 gene mutations in periodontitis. | the card15 gene encodes the nod2 protein, which is involved in intracellular recognition of bacterial products like peptidoglycan, activates inflammation and regulates apoptosis through nuclear factor-kappa b, a transcription factor that plays a central role in the innate immunity. two functional mutations, an insertion mutation at nucleotide 3020 (3020insc) and a missense mutation c2104t in the card15 gene (originally nod2 gene) have been reported to be associated with crohn's disease. our aim ... | 2004 | 15367194 |
| modulating effect of serum on the stimulation of plasminogen activator inhibitor 2 production in human gingival fibroblasts by lipopolysaccharide and interleukin-1beta. | plasminogen activator inhibitor-2 (pai-2) is an important counter proteolysis factor which helps protect tissues from inflammatory stress. the expression of pai-2 can be modulated by various inflammatory stimulants and mediators. the aim of the present study was to investigate how serum factors, might modulate the effects of lipopolysaccharide (lps) and interleukin-1beta on pai-2 production by human gingival fibroblasts. | 2004 | 15368874 |
| no link between low-grade periodontal disease and preterm birth: a pilot study in a healthy caucasian population. | 2004 | 15370639 | |
| the highly leukotoxic jp2 clone of actinobacillus actinomycetemcomitans and progression of periodontal attachment loss. | the jp2 clone of actinobacillus actinomycetemcomitans has been implicated in the etiology of periodontitis in adolescents. the aim of this two-year longitudinal study was to describe clinical attachment loss (cal) progression and to assess its association with baseline occurrence of the jp2 and non-jp2 types of a. actinomycetemcomitans. clinical re-examination of 121 adolescents in morocco was performed. progression of cal > or = 1 mm, > or = 2 mm, > or = 3 mm, and > or = 4 mm on at least one si ... | 2004 | 15381716 |
| in situ biofilm formation by multi-species oral bacteria under flowing and anaerobic conditions. | an understanding of biofilm behavior of periodontopathic bacteria is key to the development of effective oral therapies. we hypothesized that interspecies bacterial aggregates play an important role in anaerobic biofilm establishment and proliferation, and contribute to the survivability of the biofilm against therapeutic agents. the system developed in this study assessed a multi-species (streptococcus gordonii, actinobacillus actinomycetemcomitans, and fusobacterium nucleatum) biofilm formatio ... | 2004 | 15381723 |
| gingivitis as probable source of a thoracic actinomycosis due to actinomyces israelii and actinobacillus actinomycetemcomitans. | 2004 | 15383427 | |
| possible periodontal pathogens associated with clinical symptoms of periodontal disease in japanese high school students. | the purpose of this study is to investigate how the components of biofilm and clinical oral status change in adolescents and to identify specific periodontal pathogens as risk markers for the onset of periodontitis. | 2004 | 15455735 |
| clinical and microbiological effects of topical subgingival application of hyaluronic acid gel adjunctive to scaling and root planing in the treatment of chronic periodontitis. | hyaluronic acid (ha) has shown anti-inflammatory effects in gingivitis therapy. the potential benefits of local subgingival application of ha adjunctive to scaling and root planing (srp) were evaluated in this study. | 2004 | 15455740 |
| delivery of cytolethal distending toxin b induces cell cycle arrest and apoptosis in gingival squamous cell carcinoma in vitro. | the cytolethal distending toxin (cdt) from actinobacillus actinomycetemcomitans consists of three proteins, cdta, cdtb, and cdtc, which are responsible for cell cycle arrest and apoptosis. in the present study, local delivery systems of recombinant cdtb and cdtb-expressing plasmid were established using ca9-22, human gingival squamous cell carcinoma cell line. when cdtb was delivered to ca9-22 cells using a bioporter, a 32-kda protein was detected by western blotting, and g2 cell cycle arrest an ... | 2004 | 15458505 |
| characterization of plasmid por1 from ornithobacterium rhinotracheale and construction of a shuttle plasmid. | the bacterium ornithobacterium rhinotracheale has been recognized as an emerging pathogen in poultry since about 10 years ago. knowledge of this bacterium and its mechanisms of virulence is still very limited. here we report the development of a transformation system that enables genetic modification of o. rhinotracheale. the system is based on a cryptic plasmid, por1, that was derived from an o. rhinotracheale strain of serotype k. sequencing indicated that the plasmid consisted of 14,787 nucle ... | 2004 | 15466524 |
| nucleotide sequences and comparison of two large conjugative plasmids from different campylobacter species. | two large tetracycline resistance (tcr) plasmids have been completely sequenced, the ptet plasmid (45.2 kb) from campylobacter jejuni strain 81-176 and a plasmid pcc31 (44.7 kb) from campylobacter coli strain cc31 that was isolated from a human case of severe gastroenteritis in the uk. both plasmids are mosaic in structure, having homologues of genes found in a variety of different commensal and pathogenic bacteria, but nevertheless, showed striking similarities in dna sequence and overall gene ... | 2004 | 15470128 |
| need for procedural details in detection of periodontopathic bacterial dna in the atheromatous plaque by pcr. | 2004 | 15472382 | |
| analysis of the human periodontal ligament fibroblast response to the cytolethal distending toxin produced by actinobacillus actinomycetemcomitans. | a small number of diverse bacterial pathogens have been found to produce cytolethal distending toxin (cdt). much evidence has been obtained to show that cdts cause a variety of eukaryotic cells to become irreversibly blocked in the g2 phase of the cell cycle. cells which are sensitive to cdt, such as chinese hamster ovary (cho) cells, die within a few days after exposure to the toxin. recent research has shown that cdt is a potential virulence factor for actinobacillus actinomycetemcomitans, a b ... | 2001 | 15484634 |
| expression of cytolethal distending toxin genes from actinobacillus actinomycetemcomitans in escherichia coli. | 2001 | 15484635 | |
| [virulence of outer membrane proteins of periodontopathogenic bacteria]. | 2004 | 15484812 | |
| the role of iron in periodontal disease. | 1996 | 15485183 | |
| the distribution of periodontopathic bacteria among japanese children and their parents. | it is not well known how periodontopathic bacteria colonize in the oral cavity during childhood. the purpose of this study was to investigate the distribution of periodontopathic bacteria in oral cavities of children and their parents and the relationship between the bacterial findings and clinical parameters. | 2004 | 15491344 |
| periodontal pathogens in atheromatous plaques. a controlled clinical and laboratory trial. | a possible relationship between periodontitis and cardiovascular disease has been suggested. the aims of this controlled clinical study were: (i) to ascertain the presence of periodontal bacteria dna [actinobacillus actinomycetemcomitans, fusobacterium nucleatum, porphyromonas gingivalis, prevotella intermedia, tannerella forsythensis (formerly bacteroides forsythus)] in carotid atheromatous plaques and (ii) to assess the concomitant presence of the same periodontal bacteria dna, if any, in peri ... | 2004 | 15491349 |
| detection of pathogens from periodontal lesions. | to comparatively detect a. actinomycetemcomitans and f. nucleatum from periodontal and healthy sites. | 2004 | 15499445 |
| antiproliferative activity of actinobacillus (haemophilus) actinomycetemcomitans and fusobacterium nucleatum in peripheral blood mononuclear cells. | several studies indicate actinobacillus (haemophilus) actinomycetemcomitans and fusobacterium nucleatum as etiologic agents of periodontal disease. immunosuppressive factors produced by microorganisms probably contribute to the initiation and evolution of this disease. this study evaluated the antiproliferative activity of ammonium precipitate fractions of a. (h.) actinomycetemcomitans and f. nucleatum isolates from humans and marmosets both with and without periodontal disease. all a. (h.) acti ... | 2004 | 15501650 |
| intra-familial distribution of nine putative periodontopathogens in dental plaque samples analyzed by pcr. | it is of great importance to understand the distribution of periodontopathogens within family members when considering the risk of periodontitis in children. the purpose of this study was to investigate the distribution of periodontopathogens among family members. we used the polymerase chain reaction method to test 4,8, and 7 probands with healthy gingiva, gingivitis, and periodontitis, respectively, and their 60 immediate family members. plaque samples were collected from all erupted teeth sit ... | 2004 | 15508747 |
| rate of cultivable subgingival periodontopathogenic bacteria in chronic periodontitis. | although microbiological studies have identified more than 400 bacterial species in periodontal pockets, only a limited number have been implicated as periodontal pathogens. the purpose of this study was to investigate the incidence of cultivable subgingival periodontopathogenic bacteria in chronic periodontitis. bacterial samples were collected with sterile paper points from the deepest periodontal pockets ((5 mm) of 203 patients: 92 males and 111 females, aged 35-55 years. the samples were cul ... | 2004 | 15508748 |
| mmp-2 activation by actinobacillus actinomycetemcomitans supernatant in human pdl cells was corresponded with reduction of timp-2. | matrix metalloproteinase 2 (mmp-2) has been implicated to play a role in pathogenesis of periodontal disease. we recently reported that porphyromonas gingivalis supernatant could activate mmp-2 in human periodontal ligament (hpdl) cells. in this study, activation of mmp-2 by actinobacillus actinomycetemcomitans supernatant and the mechanism was investigated. | 2004 | 15533216 |
| identification of early microbial colonizers in human dental biofilm. | to elucidate the first colonizers within in vivo dental biofilm and to establish potential population shifts that occur during the early phases of biofilm formation. | 2004 | 15546422 |
| herpesviruses, the missing link between gingivitis and periodontitis? | herpesviruses appear to assume a major etiopathogenic role in various types of destructive periodontal disease. human cytomegalovirus (hcmv), epstein-barr virus (ebv) and hcmv-ebv co-infection are closely associated with disease-active periodontitis in juveniles and adults, with acute necrotizing ulcerative gingivitis in children, and with periodontal abscesses. in particular, hcmv reactivation in periodontitis lesions seems to be linked to advancing disease. hcmv infects periodontal monocytes/m ... | 2004 | 15553977 |
| methods of detection of actinobacillus actinomycetemcomitans, porphyromonas gingivalis and tannerella forsythensis in periodontal microbiology, with special emphasis on advanced molecular techniques: a review. | certain specific bacterial species from the subgingival biofilm have demonstrated aetiological relevance in the initiation and progression of periodontitis. among all the bacteria studied, three have shown the highest association with destructive periodontal diseases: actinobacillus actinomycetemcomitans (aa), porphyromonas gingivalis (pg) and tannerella forsythensis (tf). therefore, the relevance of having accurate microbiological diagnostic techniques for their identification and quantificatio ... | 2004 | 15560803 |
| subgingival plaque microbiota in saudi arabians after use of miswak chewing stick and toothbrush. | the chewing stick, the miswak, is used in many developing countries as the traditional means for oral hygiene. it is prepared from the roots, twigs and stem of salvadora persica or other alternative local plants. | 2004 | 15560804 |
| quantitative real-time polymerase chain reaction based on single copy gene sequence for detection of periodontal pathogens. | to develop a method for quantification of actinobacillus actinomycetemcomitans (aa), porphyromonas gingivalis (pg) and tannerella forsythensis (tf) from subgingival plaque samples based on taqman real-time polymerase chain reaction (pcr) technology. | 2004 | 15560805 |
| quantitative real-time polymerase chain reaction versus culture: a comparison between two methods for the detection and quantification of actinobacillus actinomycetemcomitans, porphyromonas gingivalis and tannerella forsythensis in subgingival plaque samples. | the purpose of this investigation was to validate a real-time quantitative polymerase chain reaction (pcr) assay in identifying and quantifying actinobacillus actinomycetemcomitans, porphyromonas gingivalis and tannerella forsythensis from subgingival plaque samples taken from subjects with different periodontal conditions, when compared with conventional cultural procedures. | 2004 | 15560806 |
| pnuc and the utilization of the nicotinamide riboside analog 3-aminopyridine in haemophilus influenzae. | the utilization pathway for the uptake of nad and nicotinamide riboside was previously characterized for haemophilus influenzae. we now report on the cellular location, topology, and substrate specificity of pnuc. pnuc of h. influenzae is only distantly related to pnuc of escherichia coli and salmonella enterica serovar typhimurium. when e. coli pnuc was expressed in an h. influenzae pnuc mutant, it was able to take up only nicotinamide riboside and not nicotinamide mononucleotide. therefore, we ... | 2004 | 15561822 |
| efficacy of antibiotics against periodontopathogenic bacteria within epithelial cells: an in vitro study. | periodontopathogenic bacteria can invade and survive within epithelial cells, but susceptibility of intracellular infection to antibiotics used in periodontitis treatment has not been studied to date. | 2004 | 15562909 |
| killing of periodontopathogenic bacteria by photodynamic therapy. | the aim of this study was to evaluate a new approach for killing periodontopathogenic bacteria using photodynamic therapy (pdt). | 2004 | 15562911 |
| genes involved in the synthesis and degradation of matrix polysaccharide in actinobacillus actinomycetemcomitans and actinobacillus pleuropneumoniae biofilms. | biofilms are composed of bacterial cells embedded in an extracellular polysaccharide matrix. a major component of the escherichia coli biofilm matrix is pga, a linear polymer of n-acetyl-d-glucosamine residues in beta(1,6) linkage. pga mediates intercellular adhesion and attachment of cells to abiotic surfaces. in this report, we present genetic and biochemical evidence that pga is also a major matrix component of biofilms produced by the human periodontopathogen actinobacillus actinomycetemcomi ... | 2004 | 15576769 |
| oral flora in the age of molecular biology. | the present work describes the fundamental aspects of the molecular methods that are applied to oral microbiology: probes, pcr, multiple real time pcr, 16s rdna, and proteomics. likewise, it presents the results obtained by applying these methods to the study of the bacterial flora encountered inside the mouth. by identifying 16s rdna, up to 132 known species and 215 new, unknown phylotypes have been detected inside patients s periodontal pockets. we are currently using a commercial system based ... | 2004 | 15580127 |
| periodontal diseases: microbiological considerations. | the location of plaque-associated gingivitis at the gingival portion of the tooth plays an essential role in its genesis. however, at times local and other host response modifying factors also have an influence. the pathogeny of periodontitis is more complex. the microorganisms that comprise subgingival plaque are capable of acting directly on periodontal tissues or of modifying the host response, whereas the participation of the plaque per se (normal, decreased, or increased) is as decisive as ... | 2004 | 15580139 |
| epidural abscess and osteomyelitis due to actinobacillus actinomycetemcomitans. | actinobacillus actinomycetemcomitans is a microaerophilic, fastidious gram-negative rod that most commonly causes periodontitis and odontogenic infections. we report the first case of an epidural abscess and osteomyelitis due to this organism resulting from self-extraction of carious teeth. the patient responded to surgical debridement and prolonged antimicrobial therapy with intravenous ceftriaxone. | 2004 | 15582301 |
| clinical and microbiological associations in chronic periodontitis. | chronic periodontitis is the most prevalent form of periodontal disease. the subgingival microflora has been associated with the onset and progression of the disease. the aim of this work was to establish associations and correlations between clinical indicators and subgingival plaque microbiological data in patients diagnosed with moderate or severe periodontitis. one hundred sites with a probing depth of > or = 5 mm were selected. plaque index (pi), gingival index (gi), bleeding on probing (bp ... | 2004 | 15584257 |
| deletion and purification studies to elucidate the structure of the actinobacillus actinomycetemcomitans cytolethal distending toxin. | cytolethal distending toxin (cdt) is one of the exotoxins produced by actinobacillus actinomycetemcomitans, an agent of localized aggressive periodontitis. we constructed n-terminal deletion mutants of cdta using an escherichia coli expression system and found that adelta19-47, with a deletion from asn-19 to pro-47, showed comparable cdt activity but no apparent heterogeneity of cdta. the wild-type cdt (wtcdt) and the mutant cdt (adelta19-47cdt) were purified to homogeneity by introducing a hist ... | 2004 | 15598890 |
| cell cycle arrest of human gingival fibroblasts and periodontal ligament cells by actinobacillus actinomycetemcomitans: involvement of the cytolethal distending toxin. | the cytolethal distending toxin (cdt) is produced by several gram-negative bacterial species and causes growth arrest and morphological alterations in mammalian cells. actinobacillus actinomycetemcomitans, which is involved in the pathogenesis of localized aggressive periodontitis, also produces a cdt that affects periodontal connective tissue cells. the aim of this study was to investigate in which phase of the cell cycle these cells are arrested and enlarged when challenged with a. actinomycet ... | 2004 | 15601319 |
| depolymerization of beta-1,6-n-acetyl-d-glucosamine disrupts the integrity of diverse bacterial biofilms. | polymeric beta-1,6-n-acetyl-d-glucosamine (poly-beta-1,6-glcnac) has been implicated as an escherichia coli and staphylococcus epidermidis biofilm adhesin, the formation of which requires the pgaabcd and icaabcd loci, respectively. enzymatic hydrolysis of poly-beta-1,6-glcnac, demonstrated for the first time by chromatography and mass spectrometry, disrupts biofilm formation by these species and by yersinia pestis and pseudomonas fluorescens, which possess pgaabcd homologues. | 2005 | 15601723 |
| the junctional epithelium: from health to disease. | the junctional epithelium is located at a strategically important interface between the gingival sulcus, populated with bacteria, and the periodontal soft and mineralized connective tissues that need protection from becoming exposed to bacteria and their products. its unique structural and functional adaptation enables the junctional epithelium to control the constant microbiological challenge. the antimicrobial defense mechanisms of the junctional epithelium, however, do not preclude the develo ... | 2005 | 15615869 |
| actinobacillus actinomycetemcomitans, porphyromonas gingivalis, and tannerella forsythensis are components of a polymicrobial intracellular flora within human buccal cells. | previously, we used in situ hybridization and confocal microscopy to detect the periodontal pathogens actinobacillus actinomycetemcomitans, porphyromonas gingivalis, and tannerella forsythensis within buccal epithelial cells taken directly from the mouth. this study tested the hypothesis that the intracellular flora of buccal cells is polymicrobial. mixtures containing a red fluorescent universal probe paired with green fluorescent versions of either a. actinomycetemcomitans-, p. gingivalis-, or ... | 2005 | 15615877 |
| high serum antibody levels to porphyromonas gingivalis predict myocardial infarction. | an association between coronary heart disease (chd) and clinically diagnosed periodontitis has been found in several epidemiological studies. however, seroepidemiologic evidence based on prospective data on this association is totally lacking. | 2004 | 15616414 |
| the cytolethal distending toxin induces receptor activator of nf-kappab ligand expression in human gingival fibroblasts and periodontal ligament cells. | actinobacillus actinomycetemcomitans is associated with localized aggressive periodontitis, a disease characterized by rapid loss of the alveolar bone surrounding the teeth. receptor activator of nf-kappab ligand (rankl) and osteoprotegerin (opg) are two molecules that regulate osteoclast formation and bone resorption. rankl induces osteoclast differentiation and activation, whereas opg blocks this process by acting as a decoy receptor for rankl. the purpose of this study was to investigate the ... | 2005 | 15618171 |
| abundant secretion of bioactive interleukin-1beta by human macrophages induced by actinobacillus actinomycetemcomitans leukotoxin. | actinobacillus actinomycetemcomitans produces a leukotoxin that selectively kills human leukocytes. recently, we reported that macrophages are highly sensitive to leukotoxin and that their lysis involves activation of caspase 1. in this study, we show that leukotoxin also induces the production and release of proinflammatory cytokines from human macrophages. the macrophages were challenged with leukotoxin or lipopolysaccharide (lps) from a. actinomycetemcomitans or lps from escherichia coli, and ... | 2005 | 15618184 |
| introns in the cytolethal distending toxin gene of actinobacillus actinomycetemcomitans. | in eukaryotic cells, genes are interrupted by intervening sequences called introns. introns are transcribed as part of a precursor rna that is subsequently removed by splicing, giving rise to mature mrna. however, introns are rarely found in bacteria. actinobacillus actinomycetemcomitans is a periodontal pathogen implicated in aggressive forms of periodontal disease. this organism has been shown to produce cytolethal distending toxin (cdt), which causes sensitive eukaryotic cells to become irrev ... | 2005 | 15629928 |
| clinical effects of scaling and root planing on untreated teeth. | the aim of this report is to examine whether scaling and root planing (srp) in one area of the mouth may affect periodontal improvement in untreated areas in the same patient, possibly through systemic effects of treatment. | 2005 | 15642054 |
| effectiveness of a triclosan/copolymer dentifrice on microbiological and inflammatory parameters. | according to the us surgeon general's report, "oral health in america," published in 2000, most adults in the united states show some degree of periodontal pathology, with severe periodontal diseases affecting about 14% of middle-aged adults. periodontal diseases are polymicrobial-induced inflammatory diseases, and they vary from mild gingival inflammation to severe deterioration of the periodontium, ie, loss of periodontal supportive tissues and, ultimately, tooth loss. new evidence shows that ... | 2004 | 15645886 |
| detection of dna from periodontal pathogenic bacteria in biofilm obtained from waterlines in dental units. | direct person-to-person transmission of periodontal bacteria through saliva has recently been widely reported and dental units have been demonstrated to retract saliva from patients under treatment and to release it into the mouths of subjects undergoing the next operation. in this study the presence of a group of periodontal pathogenic bacteria inside waterlines in dental units was investigated using polymerase chain reaction (pcr) based methods. briefly, 18 dental units of three different manu ... | 2004 | 15646054 |
| human atherosclerotic plaque contains viable invasive actinobacillus actinomycetemcomitans and porphyromonas gingivalis. | 2005 | 15662025 | |
| expression of major histocompatibility complex class ii and cd80 by gingival epithelial cells induces activation of cd4+ t cells in response to bacterial challenge. | hla-dr (major histocompatibility complex [mhc] class ii) is often expressed by epithelial cells in gingival tissues with periodontal disease but not by cells in healthy gingival tissues. confocal microscopic analyses revealed that gingival epithelial cells (gec) from tissue with periodontal disease express both hla-dr and b7-1 (cd80) costimulatory molecules. rat gec lines were established to elucidate the possible role of mhc class ii and b7-1 expression by gec. stimulation of a rat gec line wit ... | 2005 | 15664948 |
| [judicious use of antibiotics in dental practice]. | with the discovery of penicillin and entrance into the antibiotic era, the capability of dentists to treat dental infections have changed dramatically. many antibacterial agents have developed since, but bacterial resistance using diverse mechanisms, have increased concomitantly. since antimicrobial agents are frequently needed in dentistry, their judicious use is of prime importance. dental infections can be divided to two main groups according to the origin of the infection. first, odontogenic ... | 2004 | 15672640 |
| hacek endocarditis causing endogenous endophthalmitis and a metastatic abscess. | 2006 | 15688044 | |
| effects of an antimicrobial additive to toothbrushes on residual periodontal pathogens. | previous studies have reported the link between residual microbial contamination of toothbrushes and periodontal diseases. the goal of this pilot study was to evaluate the effects of an antimicrobial additive (microban) to toothbrushes on residual retention of periodontal pathogens. | 2004 | 15688961 |
| periodontal infections and pre-term low birth weight: a case-control study. | pre-term delivery of low-birth-weight infants [pre-term low birth weight (plbw)] remains a significant public health issue and a major cause of neonatal death and long-term health problems. there is a growing consensus that infections remote from fetal-placental unit may influence plbw infants. recent studies have suggested that maternal periodontal disease may be an independent risk factor for plbw. the purpose of the present study was to evaluate the possible link between periodontal infection ... | 2005 | 15691348 |
| serum antibody levels to actinobacillus actinomycetemcomitans predict the risk for coronary heart disease. | the association between serum antibody levels to major periodontal pathogens and coronary heart disease (chd) was analyzed in a prospective population-based study. | 2005 | 15692101 |
| relationship of actinobacillus actinomycetemcomitans serotypes to periodontal condition: prevalence and proportions in subgingival plaque. | no study available has utilized the new classification scheme (the consensus report of the american academy of periodontology 1999) to determine the prevalence of actinobacillus actinomycetemcomitans in different periodontal conditions. the purpose of this study was to investigate prevalence and proportions of a. actinomycetemcomitans serotypes in subgingival plaque samples from a young taiwanese population with aggressive periodontitis, chronic periodontitis and no periodontal disease. a total ... | 2005 | 15693826 |
| dna-dna hybridization demonstrates multiple bacteria in osteoradionecrosis. | bone necrosis secondary to radiation was previously attributed to trauma of devitalized bone and microbiological sepsis. however, conventional microbiological technique has failed to demonstrate microorganisms throughout osteoradionecrotic bone, claimed to be hypoxic, hypovascular and hypocellular. the aim of the present study was to examine such bone for bacteria using dna-dna hybridization. compared to standard culture methods this technique enables the investigation of a vast number of bacter ... | 2005 | 15695050 |
| induction of cell cycle arrest in lymphocytes by actinobacillus actinomycetemcomitans cytolethal distending toxin requires three subunits for maximum activity. | we have previously shown that actinobacillus actinomycetemcomitans produces an immunosuppressive factor encoded by the cytolethal distending toxin (cdt)b gene. in this study, we used rcdt peptides to study the contribution of each subunit to toxin activity. as previously reported, cdtb is the only cdt subunit that is capable of inducing cell cycle arrest by itself. although cdta and cdtc do not exhibit activity alone, each subunit is able to significantly enhance the ability of cdtb to induce g2 ... | 2005 | 15699156 |
| actinobacillus actinomycetemcomitans induces apoptosis in human monocytic thp-1 cells. | it has previously been reported that the murine macrophage cell line j774.1 and the human oral epithelial cell line kb undergo apoptosis as a result of actinobacillus actinomycetemcomitans infection. recent studies have demonstrated that apoptosis regulation is modulated by multiple phosphorylation of several different protein kinases, including the major subtypes of the mitogen-activated protein kinase (mapk) family. the mapk family promotes cell survival and/or proliferation in response to gro ... | 2005 | 15713614 |
| presence of actinobacillus actinomycetemcomitans on the community periodontal index (cpi) teeth in periodontally healthy individuals. | the aim of this study was to compare the prevalence of actinobacillus actinomycetemcomitans (a. actinomycetemcomitans) located at community periodontal index (cpi) teeth versus randomly selected teeth to determine if the cpi teeth are representative of this bacteria subgingival colonization in periodontally healthy young individuals. forty-four individuals between 18 and 27 years of age were included in the study (mean age 23.11 +/- 2.91 years). pooled subgingival plaque samples were collected w ... | 2005 | 15719080 |
| binding of actinobacillus actinomycetemcomitans lipopolysaccharides to peptostreptococcus micros stimulates tumor necrosis factor alpha production by macrophage-like cells. | peptostreptococcus micros is a gram-positive bacterium that has been associated with periodontitis and endodontic infections. in this study, we hypothesized that p. micros binds the immunomodulating component lipopolysaccharide derived from gram-negative bacteria to increase its capacity to stimulate cytokine production by host cells. the ability of p. micros to bind actinobacillus actinomycetemcomitans lipopolysaccharide was demonstrated by an enzyme-linked immunosorbent assay and by immunoelec ... | 2005 | 15720573 |
| impact of sample storage on detection of periodontal bacteria. | information on the impact of sample storage prior to analysis by dna methods is limited. the aim of this study was to investigate the effect of subgingival sample storage on bacterial detection and enumeration. | 2005 | 15720575 |
| susceptibility of streptococcus mutans and actinobacillus actinomycetemcomitans to bactericidal activity of human beta-defensin 3 in biological fluids. | bactericidal activity of human beta-defensin 3 (hbd-3) against streptococcus mutans and actinobacillus actinomycetemcomitans was inhibited in a dose-dependent manner by the presence of saliva and/or serum. increasing the concentration of hbd-3 partially overcame this inhibition. a fast bactericidal effect was observed against both bacterial strains, suggesting a potential therapeutic use for hbd-3 in the local treatment of oral infections. | 2005 | 15728941 |
| microbiological changes with the use of locally delivered doxycycline in the periodontal treatment of smokers. | the aim of this study was to evaluate the effect of the association of locally delivered doxycycline 10% and scaling and root planing in the subgingival plaque of smokers. | 2004 | 15732860 |
| actinobacillus actinomycetemcomitans lipopolysaccharide activates matrix metalloproteinase-2 and increases receptor activator of nuclear factor-kappab ligand expression in human periodontal ligament cells. | the lipopolysaccharide (lps) of a. actinomycetemcomitans is one of the major pathogenic factors in periodontal disease. it induces secretion of proinflammatory cytokines and is involved in alveolar bone destruction. we hypothesized that the lps of a. actinomycetemcomitans could affect the activation of matrix metalloproteinase (mmp)-2 and the expression of receptor activator of nuclear factor kappa b ligand (rankl) and osteoprotegerin in human periodontal ligament (hpdl) cells leading to the des ... | 2004 | 15732867 |
| the prevalence and pathogenic differences of porphyromonas gingivalis fima genotypes in patients with aggressive periodontitis. | the fima gene, which encodes fimbrillin (fima), is found in porphyromonas gingivalis and has been classified into six genotypes based on nucleotide sequence. p. gingivalis that possesses the type ii fima gene is prevalent in adult periodontitis. | 2005 | 15733149 |
| p38 mapk-dependent and yy1-mediated chemokine receptors ccr5 and cxcr4 up-regulation in u937 cell line infected by mycobacterium tuberculosis or actinobacillus actinomycetemcomitans. | we have found previously that the chemokine receptors ccr5 and cxcr4, which are the coreceptors of hiv, are up-regulated in human macrophage cell line u937 infected by mycobacterium tuberculosis (mtb). this suggests another possibility to explain the co-infection of mtb and hiv. in order to detect the up-regulation of ccr5 and cxcr4 as a unique phenomenon of mtb infection or a ubiquitous phenomenon of pathogenic bacteria, we investigated the expression changes of these two chemokine receptors in ... | 2005 | 15737629 |
| using photodynamic therapy to treat peri-implantitis. interview. | 2005 | 15747830 | |
| apoptotic activity and sub-cellular localization of a t4ss-associated cage-homologue in actinobacillus actinomycetemcomitans. | a potent virulence factor, cage homologue of actinobacillus actinomycetemcomitans, was identified via an expression cloning strategy and periodontitis-associated cd4(+)t-cells of a humanized mouse model. through the immuno-gold labeling with transmission electron microscopy, immunofluorescent staining, in vitro co-cultures and western blot studies, the resulting data clearly demonstrate that: (i) in cage-homologue treated human epithelia in vitro, there are ultra-structural features of plasma me ... | 2005 | 15748814 |
| the actinobacillus actinomycetemcomitans autotransporter adhesin aae exhibits specificity for buccal epithelial cells from humans and old world primates. | cells of the gram-negative periodontopathogen actinobacillus actinomycetemcomitans express a surface-exposed, outer membrane autotransporter protein, designated aae, which has been implicated in epithelial cell binding. we constructed a mutant strain of a. actinomycetemcomitans that contained a transposon insertion in the aae structural gene (aae) and tested the mutant to determine its ability to bind to buccal epithelial cells (becs) isolated from healthy volunteers. significantly fewer mutant ... | 2005 | 15784534 |
| brain abscess due to actinobacillus actinomycetemcomitans. | actinobacillus actinomycetemcomitans, a constituent of the oral flora, is a rare cause of brain abscesses. we report the case of a 47-year-old male who presented with multiple brain abscesses due to this organism, presumably originating from his poor dentition. problems met in isolating and identifying a. actinomycetemcomitans suggest that its true rate of isolation from non-oral samples may have been underestimated. | 2005 | 15799768 |
| [cloning and sequence analysis of recombinant fusion gene of escherichia coli heat-liable enterotoxin b subunit and actinobacillus actinomycetemcomitans fimbria associative protein]. | to clone the recombinant fusion gene of escherichia coli heat-liable enterotoxin b subunit (ltb) and actinobacillus actinomycetemcomitans fimbria associative protein (fap). | 2005 | 15804013 |
| cytokine responses of human gingival fibroblasts to actinobacillus actinomycetemcomitans cytolethal distending toxin. | actinobacillus actinomycetemcomitans is implicated in the pathogenesis of localized aggressive periodontitis, and has the capacity to express a cytolethal distending toxin (cdt). gingival fibroblasts (gf) are resident cells of the periodontium, which can express several osteolytic cytokines. the aims of this study were a) to investigate the role of cdt in a. actinomycetemcomitans-induced expression of osteolytic cytokines and their cognate receptors in gf and b) to determine if the previously de ... | 2005 | 15804596 |
| concurrence between the gene expression pattern of actinobacillus actinomycetemcomitans in localized aggressive periodontitis and in human epithelial cells. | actinobacillus actinomycetemcomitans is a facultatively intracellular pathogen and the aetiological agent of localized aggressive periodontitis. screening of the genome of a. actinomycetemcomitans for in vivo-induced antigen determinants previously demonstrated that the proteome of this organism differs in laboratory culture compared with conditions found during active infection. the aim of the present study was to determine whether the bacterial gene expression pattern inferred with in vivo-ind ... | 2005 | 15824431 |
| non-surgical periodontal therapy with adjunctive topical doxycycline: a double-masked, randomized, controlled multicenter study. ii. microbiological results. | topical application of active substances offers an additional option in periodontal therapy. the aim of this study was to examine the influence of the administration of a novel, biodegradable 14% doxycycline gel on microbiological findings, in connection with scaling and root planing. | 2005 | 15830639 |
| dna probe identification of bacteria colonizing internal surfaces of the implant-abutment interface: a preliminary study. | currently, there is limited knowledge concerning the specific genus and species of bacteria that may colonize internal surfaces of the implant-abutment interface (iai) of two-stage dental implants. the purpose of this study was to use dna probe analysis to identify those periodontopathic bacteria that may inhabit the internal surfaces and healing abutment screw-threads of the iai of dental implants in situ. | 2005 | 15830645 |
| elevated serum igg titer and avidity to actinobacillus actinomycetemcomitans serotype c in japanese periodontitis patients. | the purpose of this study was to characterize serum antibody responses to different serotypes of actinobacillus actinomycetemcomitans strains in various forms of periodontitis and to determine whether any specific type of a. actinomycetemcomitans was associated with any specific form of periodontitis in a japanese population. | 2005 | 15836519 |
| mutation analysis of the flp operon in actinobacillus actinomycetemcomitans. | fresh clinical isolates of the periodontal pathogen actinobacillus actinomycetemcomitans live as autoaggregates, in which cells are densely packed and embedded in an extracellular matrix composed of bundled fimbriae, exopolymers, and vesicles. the expression of fimbriae is known to be determined by the flp operon of 14 genes, flp-1-flp-2-tadv-rcpcab-tadzabcdefg. we generated mutations of each gene of this operon in a. actinomycetemcomitans strain d7s. all mutants expressed some changes in the pr ... | 2005 | 15837433 |
| treponema medium glycoconjugate inhibits activation of human gingival fibroblasts stimulated with phenol-water extracts of periodontopathic bacteria. | oral treponemes are well-known as causative agents of periodontal diseases; however, the details have not been fully clarified. here, we examined the effects of treponema medium glycoconjugate on the activation of human gingival fibroblasts using phenol-water extracts from porphyromonas gingivalis, prevotella intermedia, fusobacterium nucleatum subsp. nucleatum, and actinobacillus actinomycetemcomitans. the phenol-water extracts activated human gingival fibroblasts to mediate il-8 production, as ... | 2005 | 15840783 |
| actinobacillus actinomycetemcomitans-induced periodontal disease in mice: patterns of cytokine, chemokine, and chemokine receptor expression and leukocyte migration. | although the pathogenesis of periodontal disease (pd) is not well known, cytokines, chemotactic factors and inflammatory cells are certainly involved in the disease outcome. here, we characterized the evolution of the pd induced by actinobacillus actinomycetemcomitans in mice, showing that oral inoculation of these bacteria leads to the migration of leukocytes to periodontal tissues and marked alveolar bone resorption. we found the expression of pro-inflammatory and th1-type cytokines including ... | 2005 | 15850760 |
| microbiology of periodontal diseases: introduction. | 2005 | 15853934 | |
| in vivo induced genes in human diseases. | 2005 | 15853939 | |
| characterization of actinobacillus actinomycetemcomitans isolated from young chinese aggressive periodontitis patients. | this study characterized actinobacillus actinomycetemcomitans isolates from young chinese aggressive periodontitis patients. | 2005 | 15853973 |