Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| exploring protein lipidation with chemical biology. | 2011 | 21919527 | |
| reactive oxygen species as transducers of sphinganine-mediated cell death pathway. | long chain bases or sphingoid bases are building blocks of complex sphingolipids that display a signaling role in programmed cell death in plants. so far, the type of programmed cell death in which these signaling lipids have been demonstrated to participate is the cell death that occurs in plant immunity, known as the hypersensitive response. the few links that have been described in this pathway are: mpk6 activation, increased calcium concentrations, and reactive oxygen species (ros) generatio ... | 2011 | 21921699 |
| Characterisation of histamine-producing bacteria from farmed blackspot seabream (Pagellus bogaraveo) and turbot (Psetta maxima). | Turbot (Psetta maxima) and blackspot seabream (Pagellus bogaraveo) represent two of the most important emerging farmed fish species in European countries. However, no information of the presence and development of histamine-producing bacteria on them has been reported so far. Accordingly, the aim of this study was to isolate and identify the main histamine-producing bacteria in farmed turbot and blackspot seabream. For this study, 24 isolates (12 from turbot and 12 from blackspot seabream) were ... | 2011 | 21925757 |
| inhibitor of apoptosis (iap)-like protein lacks a baculovirus iap repeat (bir) domain and attenuates cell death in plant and animal systems. | a novel arabidopsis thaliana inhibitor of apoptosis was identified by sequence homology to other known inhibitor of apoptosis (iap) proteins. arabidopsis iap-like protein (atilp) contained a c-terminal ring finger domain but lacked a baculovirus iap repeat (bir) domain, which is essential for anti-apoptotic activity in other iap family members. the expression of atilp in hela cells conferred resistance against tumor necrosis factor (tnf)-α/actd-induced apoptosis through the inactivation of caspa ... | 2011 | 21926169 |
| rnr gene from the antarctic bacterium pseudomonas syringae lz4w, encoding a psychrophilic rnase r. | rnase r is a highly processive, hydrolytic 3'-5' exoribonuclease belonging to the rnb/rnr superfamily which plays significant roles in rna metabolism in bacteria. the enzyme was observed to be essential for growth of the psychrophilic antarctic bacterium pseudomonas syringae lz4w at a low temperature. we present results here pertaining to the biochemical properties of rnase r and the rnase r-encoding gene (rnr) locus from this bacterium. by cloning and expressing a his₆-tagged form of the p. syr ... | 2011 | 21926201 |
| contribution of siderophore systems to growth and urinary tract colonization of asymptomatic bacteriuria escherichia coli. | the molecular mechanisms that define asymptomatic bacteriuria (abu) escherichia coli colonization of the human urinary tract remain to be properly elucidated. here, we utilize abu e. coli strain 83972 as a model to dissect the contribution of siderophores to iron acquisition, growth, fitness, and colonization of the urinary tract. we show that e. coli 83972 produces enterobactin, salmochelin, aerobactin, and yersiniabactin and examine the role of these systems using mutants defective in sideroph ... | 2012 | 21930757 |
| attenuation of virulence in pathogenic bacteria using synthetic quorum-sensing modulators under native conditions on plant hosts. | quorum sensing (qs) is often critical in both pathogenic and mutualistic relationships between bacteria and their eukaryotic hosts. gram-negative bacteria typically use n-acylated l-homoserine lactone (ahl) signals for qs. we have identified a number of synthetic ahl analogues that are able to strongly modulate qs in culture-based, reporter gene assays. while informative, these assays represent idealized systems, and their relevance to qs under native conditions is often unclear. as one of our g ... | 2011 | 21932837 |
| a plasmodesmata-localized protein mediates crosstalk between cell-to-cell communication and innate immunity in arabidopsis. | plasmodesmata (pd) are thought to play a fundamental role in almost every aspect of plant life, including normal growth, physiology, and developmental responses. however, how specific signaling pathways integrate pd-mediated cell-to-cell communication is not well understood. here, we present experimental evidence showing that the arabidopsis thaliana plasmodesmata-located protein 5 (pdlp5; also known as hopw1-1-induced gene1) mediates crosstalk between pd regulation and salicylic acid-dependent ... | 2011 | 21934146 |
| a molecular insight into algal-oomycete warfare: cdna analysis of ectocarpus siliculosus infected with the basal oomycete eurychasma dicksonii. | brown algae are the predominant primary producers in coastal habitats, and like land plants are subject to disease and parasitism. eurychasma dicksonii is an abundant, and probably cosmopolitan, obligate biotrophic oomycete pathogen of marine brown algae. oomycetes (or water moulds) are pathogenic or saprophytic non-photosynthetic stramenopiles, mostly known for causing devastating agricultural and aquacultural diseases. whilst molecular knowledge is restricted to crop pathogens, pathogenic oomy ... | 2011 | 21935414 |
| Ethylene-Responsive Element-Binding Factor 5, ERF5, Is Involved in Chitin-Induced Innate Immunity Response. | Our recent work demonstrated that chitin treatment modulated the expression of 118 transcription factor (TF) genes in Arabidopsis. To investigate the potential roles of these TF in chitin signaling and plant defense, we initiated an interaction study among these TF proteins, as well as two chitin-activated mitogen-activated protein kinases (MPK3 and MPK6), using a yeast two-hybrid system. This study revealed interactions among the following proteins: three ethylene-responsive element-binding fac ... | 2012 | 21936663 |
| n-acyl-homoserine lactone confers resistance toward biotrophic and hemibiotrophic pathogens via altered activation of atmpk6. | pathogenic and symbiotic bacteria rely on quorum sensing to coordinate the collective behavior during the interactions with their eukaryotic hosts. many gram-negative bacteria use n-acyl-homoserine lactones (ahls) as signals in such communication. here we show that plants have evolved means to perceive ahls and that the length of acyl moiety and the functional group at the γ position specify the plant's response. root treatment with the n-3-oxo-tetradecanoyl-l-homoserine lactone (oxo-c14-hsl) re ... | 2011 | 21940998 |
| Cloning, protein expression and display of synthetic multi-epitope mycobacterial antigens on Salmonella typhi Ty21a cell surface. | Expressing proteins of interest as fusion to proteins of bacterial envelope is a powerful technique for biotechnological and medical applications. The synthetic gene (VacII) encoding for T-cell epitopes of selected genes of Mycobacterium tuberculosis namely, ESAT6, MTP40, 38 kDa, and MPT64 was fused with N- terminus of Pseudomonas syringae ice nucleation protein (INP) outer membrane protein. The fused genes were cloned into a bacterial expression vector pKK223-3. The recombinant protein was puri ... | 2011 | 21941936 |
| the nac domain-containing protein, gmnac6, is a downstream component of the er stress- and osmotic stress-induced nrp-mediated cell-death signaling pathway. | the endoplasmic reticulum (er) is a major signaling organelle, which integrates a variety of responses against physiological stresses. in plants, one such stress-integrating response is the n-rich protein (nrp)-mediated cell death signaling pathway, which is synergistically activated by combined er stress and osmotic stress signals. despite the potential of this integrated signaling to protect plant cells against different stress conditions, mechanistic knowledge of the pathway is lacking, and d ... | 2011 | 21943253 |
| dihydrophenylalanine: a prephenate-derived photorhabdus luminescens antibiotic and intermediate in dihydrostilbene biosynthesis. | 2,5-dihydrophenylalanine (h(2)phe) is a multipotent nonproteinogenic amino acid produced by various actinobacteria and gammaproteobacteria. although the metabolite was discovered over 40 years ago, details of its biosynthesis have remained largely unknown. we show here that l-h(2)phe is a secreted metabolite in photorhabdus luminescens cultures and a precursor of a recently described 2,5-dihydrostilbene. bioinformatic analysis suggested a candidate gene cluster for the processing of prephenate t ... | 2011 | 21944749 |
| SagS contributes to the motile-sessile switch and acts in concert with BfiSR to enable Pseudomonas aeruginosa biofilm formation. | The interaction of Pseudomonas aeruginosa with surfaces has been described as a two-stage process requiring distinct signaling events and the reciprocal modulation of small RNAs (sRNAs). However, little is known regarding the relationship between sRNA-modulating pathways active under planktonic or surface-associated growth conditions. Here, we demonstrate that SagS (PA2824), the cognate sensor of HptB, links sRNA-modulating activities via the Gac/HptB/Rsm system postattachment to the signal tran ... | 2011 | 21949078 |
| arabidopsis rtnlb1 and rtnlb2 reticulon-like proteins regulate intracellular trafficking and activity of the fls2 immune receptor. | receptors localized at the plasma membrane are critical for the recognition of pathogens. the molecular determinants that regulate receptor transport to the plasma membrane are poorly understood. in a screen for proteins that interact with the flagelin-sensitive2 (fls2) receptor using arabidopsis thaliana protein microarrays, we identified the reticulon-like protein rtnlb1. we showed that fls2 interacts in vivo with both rtnlb1 and its homolog rtnlb2 and that a ser-rich region in the n-terminal ... | 2011 | 21949153 |
| basic peptide-morpholino oligomer conjugate that is very effective in killing bacteria by gene-specific and nonspecific modes. | basic peptides covalently linked to nucleic acids, or chemically modified nucleic acids, enable the insertion of such a conjugate into bacteria grown in liquid medium and mammalian cells in tissue culture. a unique peptide, derived from human t cells, has been employed in a chemical synthesis to make a conjugate with a morpholino oligonucleotide. this new conjugate is at least 10- to 100-fold more effective than previous peptides used in altering the phenotype of host bacteria if the external gu ... | 2011 | 21949365 |
| degradation of a benzene-toluene mixture by hydrocarbon-adapted bacterial communities. | we examined the rate of degradation of a benzene-toluene mixture in aerobic microcosms prepared with samples of an aquifer that lies below a petrochemical plant (siren, uk). five samples exposed to different concentrations of benzene (from 0.6 to 317 mg l(-1)) were used. fast degradation (approx. 1-6 mg l(-1) day(-1)) of both contaminants was observed in all groundwater samples and complete degradation was recorded by the seventh day except for one sample. we also identified the microbial commun ... | 2011 | 21949494 |
| degradation of a benzene-toluene mixture by hydrocarbon-adapted bacterial communities. | we examined the rate of degradation of a benzene-toluene mixture in aerobic microcosms prepared with samples of an aquifer that lies below a petrochemical plant (siren, uk). five samples exposed to different concentrations of benzene (from 0.6 to 317 mg l(-1)) were used. fast degradation (approx. 1-6 mg l(-1) day(-1)) of both contaminants was observed in all groundwater samples and complete degradation was recorded by the seventh day except for one sample. we also identified the microbial commun ... | 2011 | 21949494 |
| nuclear dynamics during plant innate immunity. | 2011 | 21951465 | |
| nuclear dynamics during plant innate immunity. | 2011 | 21951465 | |
| novel dimeric β-helical model of an ice nucleation protein with bridged active sites. | ice nucleation proteins (inps) allow water to freeze at high subzero temperatures. due to their large size (>120 kda), membrane association, and tendency to aggregate, an experimentally-determined tertiary structure of an inp has yet to be reported. how they function at the molecular level therefore remains unknown. | 2011 | 21951648 |
| Cheating, trade-offs and the evolution of aggressiveness in a natural pathogen population. | The evolutionary dynamics of pathogens are critically important for disease outcomes, prevalence and emergence. In this study we investigate ecological conditions that may promote the long-term maintenance of virulence polymorphisms in pathogen populations. Recent theory predicts that evolution towards increased virulence can be reversed if less-aggressive social 'cheats' exploit more aggressive 'cooperator' pathogens. However, there is no evidence that social exploitation operates within natura ... | 2011 | 21951910 |
| oswrky iia transcription factors modulate rice innate immunity. | wrky transcription factors regulate diverse plant processes including responses to biotic stresses. our previous studies indicate that oswrky62, an oswrky iia subfamily member, functions as a negative regulator of the rice defense against xanthomonas oryzae pv. oryzae. here, we report that a large inverted repeat construct designed to knock down the expression of the four oswrky iia subfamily members (oswrky62, oswrky28, oswrky71, and oswrky76) leads to overexpression of all four genes and disea ... | 2010 | 21961049 |
| the jaz proteins: a crucial interface in the jasmonate signaling cascade. | jasmonates are phytohormones that regulate many aspects of plant growth, development, and defense. within the signaling cascades that are triggered by jasmonates, the jasmonate-zim domain (jaz) repressor proteins play a central role. the endogenous bioactive ja-ile conjugate mediates the binding of jaz proteins to the f-box protein coronatine insensitive1 (coi1), part of the skp1/cullin/f-box scf(coi1) ubiquitin e3 ligase complex. upon the subsequent destruction of the jaz proteins by the 26s pr ... | 2011 | 21963667 |
| identification of quantitative trait loci controlling gene expression during the innate immunity response of soybean. | microbe-associated molecular pattern-triggered immunity (mti) is an important component of the plant innate immunity response to invading pathogens. however, most of our knowledge of mti comes from studies of model systems with relatively little work done with crop plants. in this work, we report on variation in both the microbe-associated molecular pattern-triggered oxidative burst and gene expression across four soybean (glycine max) genotypes. variation in mti correlated with the level of pat ... | 2011 | 21963820 |
| ab initio structural modeling of and experimental validation for chlamydia trachomatis protein ct296 reveal structural similarity to fe(ii) 2-oxoglutarate-dependent enzymes. | chlamydia trachomatis is a medically important pathogen that encodes a relatively high percentage of proteins with unknown function. the three-dimensional structure of a protein can be very informative regarding the protein's functional characteristics; however, determining protein structures experimentally can be very challenging. computational methods that model protein structures with sufficient accuracy to facilitate functional studies have had notable successes. to evaluate the accuracy and ... | 2011 | 21965559 |
| Characterization of pyoverdine and achromobactin in Pseudomonas syringae pv. phaseolicola 1448a. | Pseudomonas syringae pv. phaseolicola 1448a (P. syringae 1448a), the causative agent of bean halo blight, is a bacterium capable of occupying diverse biological niches. Under conditions of iron starvation P. syringae 1448a secretes siderophores for active uptake of iron. The primary siderophore of P. syringae 1448a is pyoverdine, a fluorescent molecule that is assembled from amino acid precursors by non-ribosomal peptide synthetase (NRPS) enzymes. Whereas other species of Pseudomonas often exhib ... | 2011 | 21967163 |
| glutathione as a signaling molecule: another challenge to pathogens. | plants harbor a variety of signaling molecules which are members of a vast array of signaling networks in maintaining their physiological balance. the well known members up till now are salicylic acid (sa), jasmonic acid (ja), ethylene (et), abscissic acid (aba) and reactive oxygen species (ros) which are employed by plants for their adaptation to various environmental stresses in order to survive. gsh is gradually gaining importance and becoming a molecule of interest to a number of researchers ... | 2011 | 21969955 |
| a dual regulatory role of arabidopsis calreticulin-2 in plant innate immunity. | calreticulin (crt) is an endoplasmic reticulum-resident calcium-binding molecular chaperone that is highly conserved in multi-cellular eukaryotes. higher plants contain two distinct groups of crts: crt1/crt2 and crt3 isoforms. previous studies have shown that bacterial elongation factor tu receptor (efr), a pattern-recognition receptor that is responsible for pathogen-associated molecular pattern-triggered immunity, is a substrate for arabidopsis crt3, suggesting a role for crt3 in regulating pl ... | 2011 | 21974727 |
| tagetitoxin inhibits rna polymerase through trapping of the trigger loop. | tagetitoxin (tgt) inhibits multisubunit chloroplast, bacterial, and some eukaryotic rna polymerases (rnaps). a crystallographic structure of tgt bound to bacterial rnap apoenzyme shows that tgt binds near the active site but does not explain why tgt acts only at certain sites. to understand the tgt mechanism, we constructed a structural model of tgt bound to the transcription elongation complex. in this model, tgt interacts with the β' subunit trigger loop (tl), stabilizing it in an inactive con ... | 2011 | 21976682 |
| arabidopsis seedling flood-inoculation technique: a rapid and reliable assay for studying plant-bacterial interactions. | abstract: | 2011 | 21978451 |
| a conserved phd finger protein and endogenous rnai modulate insulin signaling in caenorhabditis elegans. | insulin signaling has a profound effect on longevity and the oxidative stress resistance of animals. inhibition of insulin signaling results in the activation of daf-16/foxo and skn-1/nrf transcription factors and increased animal fitness. by studying the biological functions of the endogenous rna interference factor rde-4 and conserved phd zinc finger protein zfp-1 (af10), which regulate overlapping sets of genes in caenorhabditis elegans, we identified an important role for these factors in th ... | 2011 | 21980302 |
| separable fragments and membrane tethering of arabidopsis rin4 regulate its suppression of pamp-triggered immunity. | rpm1-interacting protein 4 (rin4) is a multifunctional arabidopsis thaliana protein that regulates plant immune responses to pathogen-associated molecular patterns (pamps) and bacterial type iii effector proteins (t3es). rin4, which is targeted by multiple defense-suppressing t3es, provides a mechanistic link between pamp-triggered immunity (pti) and effector-triggered immunity and effector suppression of plant defense. here we report on a structure-function analysis of rin4-mediated suppression ... | 2011 | 21984695 |
| AtRbohF is a crucial modulator of defence-associated metabolism and a key actor in the interplay between intracellular oxidative stress and pathogenesis responses in Arabidopsis. | This work investigated the contribution of AtRbohD and AtRbohF to regulating defence-associated metabolism during three types of interaction: (i) incompatible and (ii) compatible interaction with Pseudomonas syringae; and (iii) intracellular oxidative stress in the catalase-deficient cat2 background. In all three cases, loss of function of either gene modulated the response of defence compounds. AtRbohF gene function was necessary for rapid and full induction of salicylic acid (SA) during compat ... | 2011 | 21985584 |
| RNA-Seq Analysis of a Soybean Near-Isogenic Line Carrying Bacterial Leaf Pustule-Resistant and -Susceptible Alleles. | Bacterial leaf pustule (BLP) disease is caused by Xanthomonas axonopodis pv. glycines (Xag). To investigate the plant basal defence mechanisms induced in response to Xag, differential gene expression in near-isogenic lines (NILs) of BLP-susceptible and BLP-resistant soybean was analysed by RNA-Seq. Of a total of 46 367 genes that were mapped to soybean genome reference sequences, 1978 and 783 genes were found to be up- and down-regulated, respectively, in the BLP-resistant NIL relative to the BL ... | 2011 | 21987089 |
| Cysteine homeostasis plays an essential role in plant immunity. | • Cysteine is the metabolic precursor of essential biomolecules such as vitamins, cofactors, antioxidants and many defense compounds. The last step of cysteine metabolism is catalysed by O-acetylserine(thiol)lyase (OASTL), which incorporates reduced sulfur into O-acetylserine to produce cysteine. In Arabidopsis thaliana, the main OASTL isoform OAS-A1 and the cytosolic desulfhydrase DES1, which degrades cysteine, contribute to the cytosolic cysteine homeostasis. • Meta-analysis of the transcripto ... | 2012 | 21988475 |
| accelerated cell death 2 suppresses mitochondrial oxidative bursts and modulates cell death in arabidopsis. | the arabidopsis accelerated cell death 2 (acd2) protein protects cells from programmed cell death (pcd) caused by endogenous porphyrin-related molecules like red chlorophyll catabolite or exogenous protoporphyrin ix. we previously found that during bacterial infection, acd2, a chlorophyll breakdown enzyme, localizes to both chloroplasts and mitochondria in leaves. additionally, acd2 cells show mitochondrial dysfunction. in plants with acd2 and acd2 (+) sectors, acd2 functions cell autonomously, ... | 2011 | 21988537 |
| accelerated cell death 2 suppresses mitochondrial oxidative bursts and modulates cell death in arabidopsis. | the arabidopsis accelerated cell death 2 (acd2) protein protects cells from programmed cell death (pcd) caused by endogenous porphyrin-related molecules like red chlorophyll catabolite or exogenous protoporphyrin ix. we previously found that during bacterial infection, acd2, a chlorophyll breakdown enzyme, localizes to both chloroplasts and mitochondria in leaves. additionally, acd2 cells show mitochondrial dysfunction. in plants with acd2 and acd2 (+) sectors, acd2 functions cell autonomously, ... | 2011 | 21988537 |
| arabidopsis sigma factor binding proteins are activators of the wrky33 transcription factor in plant defense. | necrotrophic pathogens are important plant pathogens that cause many devastating plant diseases. despite their impact, our understanding of the plant defense response to necrotrophic pathogens is limited. the wrky33 transcription factor is important for plant resistance to necrotrophic pathogens; therefore, elucidation of its functions will enhance our understanding of plant immunity to necrotrophic pathogens. here, we report the identification of two wrky33-interacting proteins, nuclear-encoded ... | 2011 | 21990940 |
| a critical role of staygreen/mendel's i locus in controlling disease symptom development during pseudomonas syringae pv tomato infection of arabidopsis. | production of disease symptoms represents the final phase of infectious diseases and is a main cause of crop loss and/or marketability. however, little is known about the molecular basis of disease symptom development. in this study, a genetic screening was conducted to identify arabidopsis (arabidopsis thaliana) mutants that are impaired specifically in the development of disease symptoms (leaf chlorosis and/or necrosis) after infection with the bacterial pathogen pseudomonas syringae pv tomato ... | 2011 | 21994350 |
| Genome sequence of Desulfosporosinus sp. OT, an acidophilic sulfate-reducing bacterium from copper mining waste in Norilsk, Northern Siberia. | We have sequenced the genome of Desulfosporosinus sp. OT, a Gram-positive, acidophilic sulfate-reducing Firmicute isolated from copper tailing sediment in the Norilsk mining-smelting area in Northern Siberia, Russia. This represents the first sequenced genome of a Desulfosporosinus species. The genome has a size of 5.7 Mb and encodes 6,222 putative proteins. | 2011 | 21994931 |
| fast scaffolding with small independent mixed integer programs. | assembling genomes from short read data has become increasingly popular, but the problem remains computationally challenging especially for larger genomes. we study the scaffolding phase of sequence assembly where preassembled contigs are ordered based on mate pair data. | 2011 | 21998153 |
| a genetic screen reveals arabidopsis stomatal and/or apoplastic defenses against pseudomonas syringae pv. tomato dc3000. | bacterial infection of plants often begins with colonization of the plant surface, followed by entry into the plant through wounds and natural openings (such as stomata), multiplication in the intercellular space (apoplast) of the infected tissues, and dissemination of bacteria to other plants. historically, most studies assess bacterial infection based on final outcomes of disease and/or pathogen growth using whole infected tissues; few studies have genetically distinguished the contribution of ... | 2011 | 21998587 |
| gene-counter: a computational pipeline for the analysis of rna-seq data for gene expression differences. | gene-counter is a complete perl-based computational pipeline for analyzing rna-sequencing (rna-seq) data for differential gene expression. in addition to its use in studying transcriptomes of eukaryotic model organisms, gene-counter is applicable for prokaryotes and non-model organisms without an available genome reference sequence. for alignments, gene-counter is configured for cashx, bowtie, and bwa, but an end user can use any sequence alignment/map (sam)-compliant program of preference. to a ... | 2011 | 21998647 |
| Overexpression of a cotton cyclophilin gene (GhCyp1) in transgenic tobacco plants confers dual tolerance to salt stress and Pseudomonas syringae pv. tabaci infection. | The full-length cDNA of a cyclophilin-like gene was cloned from Gossypium hirsutum using rapid amplification of cDNA ends and was designated as GhCyp1, a member of the immunophilin protein family. GhCyp1 expression level was higher in roots and stems than in other tissues of cotton, as determined by real-time reverse transcription polymerase chain reaction (RT-PCR). To characterize the GhCyp1 gene, tobacco (Nicotiana tabacum) was transformed via Agrobacterium tumefaciens with a vector to express ... | 2011 | 22000049 |
| malate-mediated carbon catabolite repression in bacillus subtilis involves the hprk/ccpa pathway. | most organisms can choose their preferred carbon source from a mixture of nutrients. this process is called carbon catabolite repression. the gram-positive bacterium bacillus subtilis uses glucose as the preferred source of carbon and energy. glucose-mediated catabolite repression is caused by binding of the ccpa transcription factor to the promoter regions of catabolic operons. ccpa binds dna upon interaction with its cofactors hpr(ser-p) and crh(ser-p). the formation of the cofactors is cataly ... | 2011 | 22001508 |
| an efficient agrobacterium-mediated transient transformation of arabidopsis. | agrobacterium tumefaciens-mediated transient transformation has been a useful procedure for characterization of proteins and their functions in plants, including analysis of protein-protein interactions. agrobacterium-mediated transient transformation of nicotiana benthamiana by leaf infiltration has been widely used due to its ease and high efficiency. however, in arabidopsis this procedure has been challenging. previous studies suggested that this difficulty was caused by plant immune response ... | 2011 | 22004025 |
| agc kinases in plant development and defense. | more than 100,000 publications demonstrate that agc kinases are important regulators of growth, metabolism, proliferation, cell divison, survival and apoptosis in mammalian systems. mutation and/or dysregulation of these kinases contribute to the pathogenesis of many human diseases, including cancer and diabetes. although agc kinases are also present in plants, little is known about their functions. we demonstrated that the agc kinase oxidative signal-inducible 1 (oxi1/agc2-1) regulate important ... | 2011 | 22005000 |
| glutathione deficiency of the arabidopsis mutant pad2-1 affects oxidative stress-related events, defense gene expression, and the hypersensitive response. | the arabidopsis (arabidopsis thaliana) phytoalexin-deficient mutant pad2-1 displays enhanced susceptibility to a broad range of pathogens and herbivorous insects that correlates with deficiencies in the production of camalexin, indole glucosinolates, and salicylic acid (sa). the pad2-1 mutation is localized in the glutamate-cysteine ligase (gcl) gene encoding the first enzyme of glutathione biosynthesis. while pad2-1 glutathione deficiency is not caused by a decrease in gcl transcripts, analysis ... | 2011 | 22007023 |
| Engineering modular and orthogonal genetic logic gates for robust digital-like synthetic biology. | Modular and orthogonal genetic logic gates are essential for building robust biologically based digital devices to customize cell signalling in synthetic biology. Here we constructed an orthogonal AND gate in Escherichia coli using a novel hetero-regulation module from Pseudomonas syringae. The device comprises two co-activating genes hrpR and hrpS controlled by separate promoter inputs, and a s(54)-dependent hrpL promoter driving the output. The hrpL promoter is activated only when both genes a ... | 2011 | 22009040 |
| arabidopsis actin depolymerizing factor4 modulates the stochastic dynamic behavior of actin filaments in the cortical array of epidermal cells. | actin filament arrays are constantly remodeled as the needs of cells change as well as during responses to biotic and abiotic stimuli. previous studies demonstrate that many single actin filaments in the cortical array of living arabidopsis thaliana epidermal cells undergo stochastic dynamics, a combination of rapid growth balanced by disassembly from prolific severing activity. filament turnover and dynamics are well understood from in vitro biochemical analyses and simple reconstituted systems ... | 2011 | 22010035 |
| Structure Function Analysis of an ADP-ribosyltransferase Type III Effector and Its RNA-binding Target in Plant Immunity. | The Pseudomonas syringae type III effector HopU1 is a mono-ADP-ribosyltransferase that is injected into plant cells by the type III protein secretion system. Inside the plant cell it suppresses immunity by modifying RNA-binding proteins including the glycine-rich RNA-binding protein GRP7. The crystal structure of HopU1 at 2.7-Å resolution reveals two unique protruding loops, L1 and L4, not found in other mono-ADP-ribosyltransferases. Site-directed mutagenesis demonstrates that these loops are es ... | 2011 | 22013065 |
| the chloroplast division mutant caa33 of arabidopsis thaliana reveals the crucial impact of chloroplast homeostasis on stress acclimation and retrograde plastid-to-nucleus signaling. | retrograde plastid-to-nucleus signaling tightly controls and coordinates the nuclear and plastid gene expression that is required for plastid biogenesis and chloroplast activity. as chloroplasts act as sensors of environmental changes, plastid-derived signaling also modulates stress responses of plants by transferring stress-related signals and altering nuclear gene expression. various mutant screens have been undertaken to identify constituents of plastid signaling pathways. almost all mutation ... | 2011 | 22014227 |
| the chloroplast division mutant caa33 of arabidopsis thaliana reveals the crucial impact of chloroplast homeostasis on stress acclimation and retrograde plastid-to-nucleus signaling. | retrograde plastid-to-nucleus signaling tightly controls and coordinates the nuclear and plastid gene expression that is required for plastid biogenesis and chloroplast activity. as chloroplasts act as sensors of environmental changes, plastid-derived signaling also modulates stress responses of plants by transferring stress-related signals and altering nuclear gene expression. various mutant screens have been undertaken to identify constituents of plastid signaling pathways. almost all mutation ... | 2011 | 22014227 |
| Miniature transposable sequences are frequently mobilized in the bacterial plant pathogen Pseudomonas syringae pv. phaseolicola. | Mobile genetic elements are widespread in Pseudomonas syringae, and often associate with virulence genes. Genome reannotation of the model bean pathogen P. syringae pv. phaseolicola 1448A identified seventeen types of insertion sequences and two miniature inverted-repeat transposable elements (MITEs) with a biased distribution, representing 2.8% of the chromosome, 25.8% of the 132-kb virulence plasmid and 2.7% of the 52-kb plasmid. Employing an entrapment vector containing sacB, we estimated tha ... | 2011 | 22016774 |
| the influence of the accessory genome on bacterial pathogen evolution. | bacterial pathogens exhibit significant variation in their genomic content of virulence factors. this reflects the abundance of strategies pathogens evolved to infect host organisms by suppressing host immunity. molecular arms-races have been a strong driving force for the evolution of pathogenicity, with pathogens often encoding overlapping or redundant functions, such as type iii protein secretion effectors and hosts encoding ever more sophisticated immune systems. the pathogens' frequent expo ... | 2011 | 22016845 |
| mRNA context dependent regulation of cytotoxic necrotizing factor 1 translation by GidA, a tRNA modification enzyme in Escherichia coli. | Cytotoxic necrotizing factor 1 (CNF1), the paradigm of Rho GTPase activating bacterial toxins has been shown to promote E. coli invasion of human brain microvascular endothelial cells (HBMEC), which constitute the blood-brain barrier, but its synthesis and secretion is unclear. In this study, we performed mini Tn5 mutagenesis screen to identify genetic requirements for CNF1 production and secretion. Transposon mutagenesis screen of meningitis-causing E. coli K1 strain RS218 revealed that CNF1 pr ... | 2012 | 22020226 |
| mRNA context dependent regulation of cytotoxic necrotizing factor 1 translation by GidA, a tRNA modification enzyme in Escherichia coli. | Cytotoxic necrotizing factor 1 (CNF1), the paradigm of Rho GTPase activating bacterial toxins has been shown to promote E. coli invasion of human brain microvascular endothelial cells (HBMEC), which constitute the blood-brain barrier, but its synthesis and secretion is unclear. In this study, we performed mini Tn5 mutagenesis screen to identify genetic requirements for CNF1 production and secretion. Transposon mutagenesis screen of meningitis-causing E. coli K1 strain RS218 revealed that CNF1 pr ... | 2012 | 22020226 |
| The extent to which methyl salicylate is required for signaling systemic acquired resistance is dependent on exposure to light after infection. | Systemic acquired resistance (SAR) is a state of heightened defense to a broad spectrum of pathogens that is activated throughout a plant following local infection. Development of SAR requires the translocation of one or more mobile signals from the site of infection through the vascular system to distal (systemic) tissues. The first such signal identified was methyl salicylate (MeSA) in tobacco (Nicotiana tabacum). Subsequent studies demonstrated that MeSA also serves as a SAR signal in Arabido ... | 2011 | 22021417 |
| Genomic and Experimental Evidence Suggests that Verrucomicrobium spinosum Interacts with Eukaryotes. | Our knowledge of pathogens and symbionts is heavily biased toward phyla containing species that are straightforward to isolate in pure culture. Novel bacterial phyla are often represented by a handful of strains, and the number of species interacting with eukaryotes is likely underestimated. Identification of predicted pathogenesis and symbiosis determinants such as the Type III Secretion System (T3SS) in the genomes of "free-living" bacteria suggests that these microbes participate in uncharact ... | 2011 | 22022322 |
| Diverting the flux of the JA pathway in Nicotiana attenuata compromises the plant's defense metabolism and fitness in nature and glasshouse. | A plant's inducible defenses against herbivores as well as certain developmental processes are known to be controlled by the jasmonic acid (JA) pathway. We have previously shown that ectopically expressing Arabidopsis thaliana JA O-methyltransferase in Nicotiana attenuata (35S-jmt) strongly reduces the herbivory-elicited jasmonate bursts by acting as metabolic sink that redirects free JA towards methylation; here we examine the consequences of this metabolic sink on N. attenuata's secondary meta ... | 2011 | 22022469 |
| regulation of disease-responsive genes mediated by epigenetic factors: interaction of arabidopsis-pseudomonas. | genes in eukaryotic organisms function within the context of chromatin, and the mechanisms that modulate the structure of chromatin are defined as epigenetic. in arabidopsis, pathogen infection induces the expression of at least one histone deacetylase, suggesting that histone acetylation/deacetylation has an important role in the pathogenic response in plants. how/whether histone methylation affects gene response to pathogen infection is unknown. to gain a better understanding of the epigenetic ... | 2011 | 22023111 |
| Identification and characterization of the Non-race specific Disease Resistance 1 (NDR1) orthologous protein in coffee. | Leaf rust, which is caused by the fungus Hemileia vastatrix (Pucciniales), is a devastating disease that affects coffee plants (Coffea arabica L.). Disadvantages that are associated with currently developed phytoprotection approaches have recently led to the search for alternative strategies. These include genetic manipulations that constitutively activate disease resistance signaling pathways. However, molecular actors of such pathways still remain unknown in C. arabica. In this study, we have ... | 2011 | 22023696 |
| silencing and heterologous expression of ppo-2 indicate a specific function of a single polyphenol oxidase isoform in resistance of dandelion (taraxacum officinale) against pseudomonas syringae pv. tomato. | dandelion (taraxacum officinale) possesses an unusually high degree of disease resistance. as this plant exhibits high polyphenol oxidase (ppo) activity and ppo have been implicated in resistance against pests and pathogens, we analyzed the potential involvement of five ppo isoenzymes in the resistance of dandelion against botrytis cinerea and pseudomonas syringae pv. tomato. only one ppo (ppo-2) was induced during infection, and ppo-2 promoter and β-glucuronidase marker gene fusions revealed st ... | 2011 | 22026646 |
| helicobacter pylori interferes with an embryonic stem cell micro rna cluster to block cell cycle progression. | abstract: | 2011 | 22027184 |
| decontamination of mda reagents for single cell whole genome amplification. | single cell genomics is a powerful and increasingly popular tool for studying the genetic make-up of uncultured microbes. a key challenge for successful single cell sequencing and analysis is the removal of exogenous dna from whole genome amplification reagents. we found that uv irradiation of the multiple displacement amplification (mda) reagents, including the phi29 polymerase and random hexamer primers, effectively eliminates the amplification of contaminating dna. the methodology is quick, s ... | 2011 | 22028825 |
| HPR1, a component of the THO/TREX complex, plays an important role in disease resistance and senescence in Arabidopsis. | ENHANCED DISEASE RESISTANCE 1 (EDR1) is a negative regulator of powdery mildew resistance, cell death and ethylene-induced senescence. To identify components involved in EDR1 signaling, we performed a forward genetic screen for edr1 suppressors. In this screen, we identified the hpr1-4 mutation, which partially suppresses edr1-mediated resistance to the powdery mildew pathogen Golovinomyces cichoracearum and mildew-induced cell death. However, the hpr1-4 mutation enhanced the ethylene-induced se ... | 2011 | 22035198 |
| excision of an unstable pathogenicity island in salmonella enterica serovar enteritidis is induced during infection of phagocytic cells. | the availability of the complete genome sequence of several salmonella enterica serovars has revealed the presence of unstable genetic elements in these bacteria, such as pathogenicity islands and prophages. this is the case of salmonella enterica serovar enteritidis (s. enteritidis), a bacterium that causes gastroenteritis in humans and systemic infection in mice. the whole genome sequence analysis for s. enteritidis unveiled the presence of several genetic regions that are absent in other salm ... | 2011 | 22039432 |
| high throughput chemical screening supports the involvement of ca2+ in cyclic nucleotide-gated ion channel-mediated programmed cell death in arabidopsis. | recently, we reported the role of arabidopsis cyclic nucleotide-gated ion channel (atcngc) 11 and 12 in ca2+-dependent physiological responses. atcngc11 and 12 have been reported to be involved in plant immunity, but whether these channels play additional physiological roles was not clear before. using single and double knockout mutants, we have found that these channels play significant roles in ca2+ signaling, which mediates several physiological processes, such as gravitropic bending and sene ... | 2011 | 22041991 |
| fungicidal activities and mechanisms of action of pseudomonas syringae pv. syringae lipodepsipeptide syringopeptins 22a and 25a. | the plant-associated bacterium pseudomonas syringae pv. syringae simultaneously produces two classes of metabolites: the small cyclic lipodepsinonapeptides such as the syringomycins and the larger cyclic lipodepsipeptide syringopeptins sp22 or sp25. the syringomycins inhibit a broad spectrum of fungi (but particularly yeasts) by lipid-dependent membrane interaction. the syringopeptins are phytotoxic and inhibitory to gram-positive bacteria. in this study, the fungicidal activities of two major s ... | 2011 | 22046175 |
| the expression pattern of the picea glauca defensin 1 promoter is maintained in arabidopsis thaliana, indicating the conservation of signalling pathways between angiosperms and gymnosperms. | a 1149 bp genomic fragment corresponding to the 5' non-coding region of the pgd1 (picea glauca defensin 1) gene was cloned, characterized, and compared with all arabidopsis thaliana defensin promoters. the cloned fragment was found to contain several motifs specific to defence or hormonal response, including a motif involved in the methyl jasmonate reponse, a fungal elicitor responsive element, and tc-rich repeat cis-acting element involved in defence and stress responsiveness. a functional anal ... | 2011 | 22048038 |
| the expression pattern of the picea glauca defensin 1 promoter is maintained in arabidopsis thaliana, indicating the conservation of signalling pathways between angiosperms and gymnosperms. | a 1149 bp genomic fragment corresponding to the 5' non-coding region of the pgd1 (picea glauca defensin 1) gene was cloned, characterized, and compared with all arabidopsis thaliana defensin promoters. the cloned fragment was found to contain several motifs specific to defence or hormonal response, including a motif involved in the methyl jasmonate reponse, a fungal elicitor responsive element, and tc-rich repeat cis-acting element involved in defence and stress responsiveness. a functional anal ... | 2011 | 22048038 |
| a highly conserved nb-lrr encoding gene cluster effective against setosphaeria turcica in sorghum. | the fungal pathogen setosphaeria turcica causes turcicum or northern leaf blight disease on maize, sorghum and related grasses. a prevalent foliar disease found worldwide where the two host crops, maize and sorghum are grown. the aim of the present study was to find genes controlling the host defense response to this devastating plant pathogen. a cdna-aflp approach was taken to identify candidate sequences, which functions were further validated via virus induced gene silencing (vigs), and real- ... | 2011 | 22050783 |
| Two small RNAs, CrcY and CrcZ, act in concert to sequester the Crc global regulator in Pseudomonas putida, modulating catabolite repression. | The Crc protein is a translational repressor that recognizes a specific target at some mRNAs, controlling catabolite repression and co-ordinating carbon metabolism in pseudomonads. In Pseudomonas aeruginosa, the levels of free Crc protein are controlled by CrcZ, a sRNA that sequesters Crc, acting as an antagonist. We show that, in Pseudomonas putida, the levels of free Crc are controlled by CrcZ and by a novel 368 nt sRNA named CrcY. CrcZ and CrcY, which contain six potential targets for Crc, we ... | 2012 | 22053874 |
| hopas1 recognition significantly contributes to arabidopsis nonhost resistance to pseudomonas syringae pathogens. | • plant immunity is activated by sensing either conserved microbial signatures, called pathogen/microbe-associated molecular patterns (p/mamps), or specific effectors secreted by pathogens. however, it is not known why most microbes are nonpathogenic in most plant species. • nonhost resistance (nhr) consists of multiple layers of innate immunity and protects plants from the vast majority of potentially pathogenic microbes. effector-triggered immunity (eti) has been implicated in race-specific di ... | 2012 | 22053875 |
| Proteomic analysis of colony morphology variants of Burkholderia pseudomallei defines a role for the arginine deiminase system in bacterial survival. | Colony morphology variation of Burkholderia pseudomallei is a notable feature of a proportion of primary clinical cultures from patients with melioidosis. Here, we examined the hypothesis that colony morphology switching results in phenotypic changes associated with enhanced survival under adverse conditions. We generated isogenic colony morphology types II and III from B. pseudomallei strain 153 type I, and compared their protein expression profiles using 2D gel electrophoresis. Numerous protei ... | 2012 | 22062159 |
| Conserved Structural Mechanisms for Autoinhibition in IpaH Ubiquitin Ligases. | The IpaH family of novel E3 ligase (NEL) enzymes occur in a variety of pathogenic and commensal bacteria that interact with eukaryotic hosts. We demonstrate that the leucine-rich repeat (LRR) substrate recognition domains of different IpaH enzymes autoinhibit the enzymatic activity of the adjacent catalytic novel E3 ligase domain by two distinct but conserved structural mechanisms. Autoinhibition is required for the in vivo biological activity of two IpaH enzymes in a eukaryotic model system. Au ... | 2012 | 22065585 |
| Conserved Structural Mechanisms for Autoinhibition in IpaH Ubiquitin Ligases. | The IpaH family of novel E3 ligase (NEL) enzymes occur in a variety of pathogenic and commensal bacteria that interact with eukaryotic hosts. We demonstrate that the leucine-rich repeat (LRR) substrate recognition domains of different IpaH enzymes autoinhibit the enzymatic activity of the adjacent catalytic novel E3 ligase domain by two distinct but conserved structural mechanisms. Autoinhibition is required for the in vivo biological activity of two IpaH enzymes in a eukaryotic model system. Au ... | 2012 | 22065585 |
| phytoplasma protein effector sap11 enhances insect vector reproduction by manipulating plant development and defense hormone biosynthesis. | phytoplasmas are insect-transmitted phytopathogenic bacteria that can alter plant morphology and the longevity and reproduction rates and behavior of their insect vectors. there are various examples of animal and plant parasites that alter the host phenotype to attract insect vectors, but it is unclear how these parasites accomplish this. we hypothesized that phytoplasmas produce effectors that modulate specific targets in their hosts leading to the changes in plant development and insect perfor ... | 2011 | 22065743 |
| a photolyase-like protein from agrobacterium tumefaciens with an iron-sulfur cluster. | photolyases and cryptochromes are evolutionarily related flavoproteins with distinct functions. while photolyases can repair uv-induced dna lesions in a light-dependent manner, cryptochromes regulate growth, development and the circadian clock in plants and animals. here we report about two photolyase-related proteins, named phra and phrb, found in the phytopathogen agrobacterium tumefaciens. phra belongs to the class iii cyclobutane pyrimidine dimer (cpd) photolyases, the sister class of plant ... | 2011 | 22066008 |
| Defence responses of Arabidopsis thaliana to infection by Pseudomonas syringae are regulated by the circadian clock. | The circadian clock allows plants to anticipate predictable daily changes in abiotic stimuli, such as light; however, whether the clock similarly allows plants to anticipate interactions with other organisms is unknown. Here we show that Arabidopsis thaliana (Arabidopsis) has circadian clock-mediated variation in resistance to the virulent bacterial pathogen Pseudomonas syringae pv. tomato DC3000 (Pst DC3000), with plants being least susceptible to infection in the subjective morning. We suggest ... | 2011 | 22066021 |
| Two Arabidopsis guard cell-preferential MAPK genes, MPK9 and MPK12, function in biotic stress response. | Abscisic acid (ABA) plays a major role in plant development and adaptation to severe environmental conditions. ABA evokes cellular events to regulate stomatal apertures and thus contributes to the plant's ability to respond to abiotic stresses. Reactive oxygen species (ROS) are produced in response to ABA and mediate ABA-induced stomatal closure. We have shown that two MAP kinases, MPK9 and MPK12, are highly and preferentially expressed in guard cells and function as positive regulators of ROS-m ... | 2011 | 22067989 |
| toxins and secretion systems of photorhabdus luminescens. | photorhabdus luminescens is a nematode-symbiotic, gram negative, bioluminescent bacterium, belonging to the family of enterobacteriaceae. recent studies show the importance of this bacterium as an alternative source of insecticides, as well as an emerging human pathogen. various toxins have been identified and characterized in this bacterium. these toxins are classified into four major groups: the toxin complexes (tcs), the photorhabdus insect related (pir) proteins, the "makes caterpillars flop ... | 2010 | 22069636 |
| cycle inhibiting factors (cifs): cyclomodulins that usurp the ubiquitin-dependent degradation pathway of host cells. | cycle inhibiting factors (cifs) are type iii secreted effectors produced by diverse pathogenic bacteria. cifs are "cyclomodulins" that inhibit the eukaryotic host cell cycle and also hijack other key cellular processes such as those controlling the actin network and apoptosis. this review summarizes current knowledge on cif since its first characterization in enteropathogenic escherichia coli, the identification of several xenologues in distant pathogenic bacteria, to its structure elucidation a ... | 2011 | 22069713 |
| modes of action of microbially-produced phytotoxins. | some of the most potent phytotoxins are synthesized by microbes. a few of these share molecular target sites with some synthetic herbicides, but many microbial toxins have unique target sites with potential for exploitation by the herbicide industry. compounds from both non-pathogenic and pathogenic microbes are discussed. microbial phytotoxins with modes of action the same as those of commercial herbicides and those with novel modes of action of action are covered. examples of the compounds dis ... | 2011 | 22069756 |
| Chemical and Metabolic Aspects of Antimetabolite Toxins Produced by Pseudomonas syringae Pathovars. | Pseudomonas syringae is a phytopathogenic bacterium present in a wide variety of host plants where it causes diseases with economic impact. The symptoms produced by Pseudomonas syringae include chlorosis and necrosis of plant tissues, which are caused, in part, by antimetabolite toxins. This category of toxins, which includes tabtoxin, phaseolotoxin and mangotoxin, is produced by different pathovars of Pseudomonas syringae. These toxins are small peptidic molecules that target enzymes of amino a ... | 2011 | 22069758 |
| SAG101 forms a ternary complex with EDS1 and PAD4 and is required for resistance signaling against turnip crinkle virus. | EDS1, PAD4, and SAG101 are common regulators of plant immunity against many pathogens. EDS1 interacts with both PAD4 and SAG101 but direct interaction between PAD4 and SAG101 has not been detected, leading to the suggestion that the EDS1-PAD4 and EDS1-SAG101 complexes are distinct. We show that EDS1, PAD4, and SAG101 are present in a single complex in planta. While this complex is preferentially nuclear localized, it can be redirected to the cytoplasm in the presence of an extranuclear form of E ... | 2011 | 22072959 |
| Multiple candidate effectors from the oomycete pathogen Hyaloperonospora arabidopsidis suppress host plant immunity. | Oomycete pathogens cause diverse plant diseases. To successfully colonize their hosts, they deliver a suite of effector proteins that can attenuate plant defenses. In the oomycete downy mildews, effectors carry a signal peptide and an RxLR motif. Hyaloperonospora arabidopsidis (Hpa) causes downy mildew on the model plant Arabidopsis thaliana (Arabidopsis). We investigated if candidate effectors predicted in the genome sequence of Hpa isolate Emoy2 (HaRxLs) were able to manipulate host defenses i ... | 2011 | 22072967 |
| use of low-coverage, large-insert, short-read data for rapid and accurate generation of enhanced-quality draft pseudomonas genome sequences. | next-generation genomic technology has both greatly accelerated the pace of genome research as well as increased our reliance on draft genome sequences. while groups such as the genomics standards consortium have made strong efforts to promote genome standards there is a still a general lack of uniformity among published draft genomes, leading to challenges for downstream comparative analyses. this lack of uniformity is a particular problem when using standard draft genomes that frequently have ... | 2011 | 22073286 |
| alkamides activate jasmonic acid biosynthesis and signaling pathways and confer resistance to botrytis cinerea in arabidopsis thaliana. | alkamides are fatty acid amides of wide distribution in plants, structurally related to n-acyl-l-homoserine lactones (ahls) from gram-negative bacteria and to n- acylethanolamines (naes) from plants and mammals. global analysis of gene expression changes in arabidopsis thaliana in response to n-isobutyl decanamide, the most highly active alkamide identified to date, revealed an overrepresentation of defense-responsive transcriptional networks. in particular, genes encoding enzymes for jasmonic a ... | 2011 | 22076141 |
| Fortunella margarita Transcriptional Reprogramming Triggered by Xanthomonas citri subsp. citri. | ABSTRACT: | 2011 | 22078099 |
| meta-analytic approach to the accurate prediction of secreted virulence effectors in gram-negative bacteria. | many pathogens use a type iii secretion system to translocate virulence proteins (called effectors) in order to adapt to the host environment. to date, many prediction tools for effector identification have been developed. however, these tools are insufficiently accurate for producing a list of putative effectors that can be applied directly for labor-intensive experimental verification. this also suggests that important features of effectors have yet to be fully characterized. | 2011 | 22078363 |
| disinfection of gram-negative and gram-positive bacteria using dynajets® hydrodynamic cavitating jets. | cavitating jet technologies (dynajets®) were investigated as a means of disinfection of gram-negative escherichia coli, klebsiellapneumoniae, pseudomonas syringae, and pseudomonas aeruginosa, and gram-positive bacillus subtilis. the hydrodynamic cavitating jets were found to be very effective in reducing the concentrations of all of these species. in general, the observed rates of disinfection of gram-negative species were higher than for gram-positive species. however, different gram-negative s ... | 2011 | 22079473 |
| Genome-wide transcriptome analysis of the plant pathogen Xanthomonas identifies sRNAs with putative virulence functions. | The Gram-negative plant-pathogenic bacterium Xanthomonas campestris pv. vesicatoria (Xcv) is an important model to elucidate the mechanisms involved in the interaction with the host. To gain insight into the transcriptome of the Xcv strain 85-10, we took a differential RNA sequencing (dRNA-seq) approach. Using a novel method to automatically generate comprehensive transcription start site (TSS) maps we report 1421 putative TSSs in the Xcv genome. Genes in Xcv exhibit a poorly conserved -10 promo ... | 2011 | 22080557 |
| Genome-wide transcriptome analysis of the plant pathogen Xanthomonas identifies sRNAs with putative virulence functions. | The Gram-negative plant-pathogenic bacterium Xanthomonas campestris pv. vesicatoria (Xcv) is an important model to elucidate the mechanisms involved in the interaction with the host. To gain insight into the transcriptome of the Xcv strain 85-10, we took a differential RNA sequencing (dRNA-seq) approach. Using a novel method to automatically generate comprehensive transcription start site (TSS) maps we report 1421 putative TSSs in the Xcv genome. Genes in Xcv exhibit a poorly conserved -10 promo ... | 2011 | 22080557 |
| the arabidopsis glucosyltransferase ugt76b1 conjugates isoleucic acid and modulates plant defense and senescence. | plants coordinate and tightly regulate pathogen defense by the mostly antagonistic salicylate (sa)- and jasmonate (ja)-mediated signaling pathways. here, we show that the previously uncharacterized glucosyltransferase ugt76b1 is a novel player in this sa-ja signaling crosstalk. ugt76b1 was selected as the top stress-induced isoform among all 122 members of the arabidopsis thaliana ugt family. loss of ugt76b1 function leads to enhanced resistance to the biotrophic pathogen pseudomonas syringae an ... | 2011 | 22080599 |
| repression of sucrose/ultraviolet b light-induced flavonoid accumulation in microbe-associated molecular pattern-triggered immunity in arabidopsis. | recognition of microbe-associated molecular patterns (mamps) leads to the generation of mamp-triggered immunity (mti), which restricts the invasion and propagation of potentially infectious microbes. it has been described that the perception of different bacterial and fungal mamps causes the repression of flavonoid induction upon light stress or sucrose application. however, the functional significance of this mti-associated signaling output remains unknown. in arabidopsis (arabidopsis thaliana) ... | 2011 | 22080602 |
| repression of sucrose/ultraviolet b light-induced flavonoid accumulation in microbe-associated molecular pattern-triggered immunity in arabidopsis. | recognition of microbe-associated molecular patterns (mamps) leads to the generation of mamp-triggered immunity (mti), which restricts the invasion and propagation of potentially infectious microbes. it has been described that the perception of different bacterial and fungal mamps causes the repression of flavonoid induction upon light stress or sucrose application. however, the functional significance of this mti-associated signaling output remains unknown. in arabidopsis (arabidopsis thaliana) ... | 2011 | 22080602 |
| cj1386 is an ankyrin-containing protein involved in heme trafficking to catalase in campylobacter jejuni. | campylobacter jejuni, a microaerophilic bacterium, is the most frequent cause of human bacterial gastroenteritis. c. jejuni is exposed to harmful reactive oxygen species (ros) produced during its own normal metabolic processes and during infection from the host immune system and from host intestinal microbiota. these ros will damage dna and proteins and cause peroxidation of lipids. consequently, identifying ros defense mechanisms is important for understanding how campylobacter survives this en ... | 2012 | 22081390 |