Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| immune activation by mycobacterium tuberculosis in hiv-infected and -uninfected subjects. | this study investigates the influence of mycobacterium tuberculosis infection on immune activation biomarkers, both in hiv-infected and -uninfected subjects. | 2017 | 27532475 |
| stool microbiome reveals diverse bacterial ureases as confounders of oral urea breath testing for helicobacter pylori and mycobacterium tuberculosis in bamako, mali. | detection of bacterial urease activity has been utilized successfully to diagnose helicobacter pylori (h. pylori). while mycobacterium tuberculosis (m. tuberculosis) also possesses an active urease, it is unknown whether detection of mycobacterial urease activity by oral urease breath test (ubt) can be exploited as a rapid point of care biomarker for tuberculosis (tb) in humans. we enrolled 34 individuals newly diagnosed with pulmonary tb and 46 healthy subjects in bamako, mali and performed ora ... | 2016 | 27532494 |
| [clinical and x-ray changes in patients with new-onset multidrug-resistant mycobacterium pulmonary tuberculosis in the early use of a reserve chemotherapy regimen according to the tb-biochip test system data]. | clinical and x-ray changes were comparatively studied in new-onset tuberculosis patients excreting susceptible mycobacterium tuberculosis (mbt) and mutidrug resistant (mdr) mbt. eighty tuberculosis patients who had not received antituberculous drugs were followed up. before chemotherapy, sputum was tested in all cases, by using the tb-biochip system, to determine drug susceptibility of mbt. according to the results of microchip diagnosis, the patients were divided into 2 groups: 1) patients with ... | 2010 | 27534019 |
| [effect of liquid probiotics on the growth of mycobacterium tuberculosis in vitro]. | the mycobacterium tuberculosis (mbt) culture isolated from the sputum of 25 patients was experimentally studied. the probiotics were tested for their bacterium-neutralizing effect on a dense luwenstein-jensen medium, by using the absolute concentration method. the nature of their bactericidal and bacteriostatic effects was assessed from the presence and intensity of mbt growth on day 21 after inoculation. in most cases, the probiotics exerted an inhibitory effect on mbt growth. the latter was co ... | 2010 | 27534022 |
| [the sensitivity and specificity of the molecular genotype mtbdrpl us assay (hain lifescience gmbh, germany) for rapid diagnosis of drug susceptibility in mycobacterium tuberculosis on sputum specimens]. | the efficiency of the molecular genotype mtbdrplus (hain lifescience gmbh, germany) assay was evaluated for the rapid diagnosis of rifampicin and isoniazid susceptibility in mycobacterium tuberculosis (mbt) on 168 sputum specimens from patients with pulmonary tuberculosis in the samara region. the interpretable results from the molecular genetic assay were obtained for 91.7% of the specimens with the rates significantly higher in the sputum specimens graded 2+ and 3+ than in those graded 1+. the ... | 2010 | 27534023 |
| [simulation of chronic tuberculosis infection in mice by intraperitoneal inoculation and the testing of aminoguanidine-induced activation of rpf gene/deletion mutants of the h37rv strain]. | intraperitoneal inoculation of mice with the virulent strain h37rv of mycobacterium tuberculosis led to the development of chronic tuberculosis for a long time. the developed model of chronic tuberculosis was used to study tuberculous infection caused by rpf gene knockout mutants of the h37rv strain. these mutant strains were unable to produce proteins that determined the transition of quies- cent to active forms of the pathogen of tuberculosis in vitro. unlike the wild-type m. tuberculosis h37r ... | 2010 | 27534027 |
| [the current aspects of diagnosis and differential diagnosis of complications after bcg vaccination]. | whether the diagnosis of bcg complications might be verified by molecular genetic methods, such as polymerase chain reaction (pcr) and sequencing, was studied. pcr could identify mycobacterium tuberculosis as m. bovis strain bcg and increase the detection rate of m. tuberculosis by 38% as compared with traditional microbiological assays, which provides a means of recommending the method to verify the diagnosis of bcg complications. | 2010 | 27534052 |
| [low-intensity laser therapy in the prophylactic treatment of tuberculin hyperergic children]. | the results of sanatorium treatment were analyzed in 156 children and adolescents aged 11-17 years at high risk for tuberculosis. all the patients had been long infected with mycobacterium tuberculosis with hyperergic tuberculin sensitivity; they had comorbidity and were susceptible to frequent respiratory diseases. a course of percutaneous low-intensity laser irradiation was incorporated as a new method into a complex of prophylactic treatment. the time course of changes in tuberculin sensitivi ... | 2010 | 27534057 |
| adaptation of mycobacterium tuberculosis to impaired host immunity in hiv-infected patients. | it is unknown whether immunosuppression influences the physiologic state of mycobacterium tuberculosis in vivo. we evaluated the impact of host immunity by comparing m. tuberculosis and human gene transcription in sputum between human immunodeficiency virus (hiv)-infected and uninfected patients with tuberculosis. | 2016 | 27534685 |
| drug-resistant tuberculosis in central mozambique: the role of a rapid genotypic susceptibility testing. | genotypic molecular testing may be very helpful for tuberculosis (tb) drug-resistance surveillance and for treatment guidance in low resource settings. | 2016 | 27534745 |
| development of one-tube multiplex polymerase chain reaction (pcr) for detecting mycobacterium bovis. | a multiplex pcr (m-pcr) with primers targeting the 16s rrna, rv3873 and a 12.7-kb fragment in the genomes of a mycobacterium tuberculosis complex was designed for the differential diagnosis of m. tuberculosis, m. bovis, m. bovis bcg and non-tuberculosis mycobacterium (ntm). the specificity of this assay was 100%, and the detection limit was 15 pg of genomic dna. of the 206 blinded clinical samples, the detection rate of m. bovis infection by m-pcr was lower than that of the interferon gamma (ifn ... | 2017 | 27534910 |
| how old are bacterial pathogens? | only few molecular studies have addressed the age of bacterial pathogens that infected humans before the beginnings of medical bacteriology, but these have provided dramatic insights. the global genetic diversity of helicobacter pylori, which infects human stomachs, parallels that of its human host. the time to the most recent common ancestor (tmrca) of these bacteria approximates that of anatomically modern humans, i.e. at least 100 000 years, after calibrating the evolutionary divergence withi ... | 2016 | 27534956 |
| a case of testicular tuberculosis mimicking malignancy in a healthy young man. | genitourinary tuberculosis represents a form of extra-pulmonary tuberculosis that occurs in the kidneys, ureters, seminal vesicles, prostate, testis, vas deferens, and epididymis. isolated testicular involvement is unusual, and differential diagnosis includes testicular tumor, acute infection, infarction, and granulomatous infection. we report a case of a 36-year-old ecuadorian man residing in new york, new york, who presented with a painful scrotal mass, weight loss, and purulent discharge from ... | 2016 | 27536355 |
| construction of a novel dna vaccine candidate encoding an hspx-ppe44-esxv fusion antigen of mycobacterium tuberculosis. | mycobacterium tuberculosis is the causative agent of tuberculosis (tb). bacille calmette-guerin (bcg) vaccine, is not effective in adults, therefore, many efforts have been made to produce an effective adult tb vaccine. the aim of this study was to develop a new tuberculosis dna vaccine candidate encoding a recombinant hspx-ppe44-esxv fusion antigen of m. tuberculosis. | 2016 | 27536702 |
| design, synthesis, structural elucidation, pharmacological evaluation of metal complexes with pyrazoline derivatives. | a bioactive pyrazoline derivatives have been synthesized by the base-catalyzed claisen-schmidt condensation of imidazole-2-carboxaldehyde with 1-acetyl-2-hydroxynaphthalene followed by cyclization with phenylhydrazine (l(1))/2,3-dimethylphenylhydrazine (l(2)) and 3-nitrophenylhydrazine (l(3)). the metal(ii) complexes [ni(ii), co(ii), cu(ii) and zn(ii)] were formed by reacting the corresponding metal acetates with the ligands. all complexes were characterized by elemental analyses, electronic, ir ... | 2016 | 27537519 |
| polishing surgical metal pieces, granulomatosis and mineralogical analysis. | this report describes the case of a 44-year-old man with pulmonary nodules whose histological analysis initially suggested tuberculosis. the mycobacterium tuberculosis (mt) culture was negative and a questionnaire revealed a professional activity of brushing and polishing surgical instruments without any protection for 7 years. a mineralogical analysis by optical and electron microscopy was performed on both a healthy lung tissue biopsy and a lung nodule in a paraffin block. electron microscopy ... | 2016 | 27537720 |
| n-alkoxyphenylhydroxynaphthalenecarboxamides and their antimycobacterial activity. | a series of nineteen n-(alkoxyphenyl)-2-hydroxynaphthalene-1-carboxamides and a series of their nineteen positional isomers n-(alkoxyphenyl)-1-hydroxynaphthalene-2-carboxamides were prepared and characterized. primary in vitro screening of all the synthesized compounds was performed against mycobacterium tuberculosis h37ra, m. kansasii and m. smegmatis. screening of the cytotoxicity of the compounds was performed using human monocytic leukemia thp-1 cells. some of the tested compounds showed ant ... | 2016 | 27537867 |
| characterization of myma protein as a flavin-containing monooxygenase and as a target of isoniazid. | tuberculosis is a global health problem especially with the emergence of drug-resistant mycobacterium tuberculosis strains, creating an urgent need to identify new drug targets. the mycobacterial cell wall is an attractive target for chemotherapeutic agents. gene products of myma operon are known to be required for the maintenance of cell wall and play an important role in persistence, thus making them important drug targets. this study was undertaken to biochemically characterize the myma as a ... | 2017 | 27539124 |
| genotypic detection of rpob and katg gene mutations associated with rifampicin and isoniazid resistance in mycobacterium tuberculosis isolates: a local scenario (kelantan). | drug resistant tuberculosis (dr-tb) remains a public health issue that is of major concern on a global scale. the characterisation of clinical isolates may provide key information regarding the underlying mechanisms of drug resistance, and helps to augment therapeutic options. this study aims to evaluate the frequency of gene mutations associated with rifampicin (rif) and isoniazid (inh) resistance among nine clinical isolates. | 2016 | 27540322 |
| binding sites for acylated trehalose analogs of glycolipid ligands on an extended carbohydrate recognition domain of the macrophage receptor mincle. | the macrophage receptor mincle binds to trehalose dimycolate on the surface of mycobacterium tuberculosis signaling initiated by this interaction leads to cytokine production, which underlies the ability of mycobacteria to evade the immune system and also to function as adjuvants. in previous work the mechanism for binding of the sugar headgroup of trehalose dimycolate to mincle has been elucidated, but the basis for enhanced binding to glycolipid ligands, in which hydrophobic substituents are a ... | 2016 | 27542410 |
| strain differentiation of mycobacterium tuberculosis for epidemiology in northwest of iran. | tuberculosis is a major health problem throughout the world and there are still a great number of people with the disease. planning for controlling tuberculosis is required to identify the sources of infection and screening for the disease. the aim of this study is the differentiation of mycobacterium tuberculosis strains for better understands the spread of the disease in north west iran. in this study, 194 positive cultures of m. tuberculosis in north west iran were evaluated by the miru-vntr ... | 2016 | 27545209 |
| proteomic and morphological changes produced by subinhibitory concentration of isoniazid in mycobacterium tuberculosis. | to study the proteomic and morphological changes in mycobacterium tuberculosis h37rv exposed to subinhibitory concentration of isoniazid (inh). | 2016 | 27545345 |
| rifapentine for the treatment of latent tuberculosis. | the goal of this article is to review the use of rifapentine in the treatment of latent tuberculosis infection (ltbi). controlling ltbi is an important part of the global strategy to end the spread of tuberculosis. rifapentine's potent sterilizing effect against mycobacterium tuberculosis combined with its long half-life make it an attractive ltbi treatment option. | 2016 | 27545595 |
| new tests for detection of mycobacterium tuberculosis infection: sufficient to meet the who 2035 targets? | 2016 | 27545599 | |
| mycobacterial whib6 differentially regulates esx-1 and the dos regulon to modulate granuloma formation and virulence in zebrafish. | during the course of infection, mycobacterium tuberculosis (mtb) is exposed to diverse redox stresses that trigger metabolic and physiological changes. how these stressors are sensed and relayed to the mtb transcriptional apparatus remains unclear. here, we provide evidence that whib6 differentially regulates the esx-1 and dosr regulons through its fe-s cluster. when challenged with no, whib6 continually activates expression of the dosr regulons but regulates esx-1 expression through initial act ... | 2016 | 27545883 |
| the bcgδbcg1419c strain, which produces more pellicle in vitro, improves control of chronic tuberculosis in vivo. | mycobacterium tuberculosis (mtb) has been a threat to humans since ancient times, and it is the main causative agent of tuberculosis (tb). until today, the only licensed vaccine against mtb is the live attenuated m. bovis bacillus calmette-guérin (bcg), which has variable levels of protection against the pulmonary form of infection. the quest for a new vaccine is a priority given the rise of multidrug-resistant mtb around the world, as well as the tremendous burden imposed by latent tb. the obje ... | 2016 | 27546876 |
| tuberculosis and pulmonary candidiasis co-infection present in a previously healthy patient. | the coexistance among fungal pathogens and tuberculosis pulmonary is a clinical condition that generally occurs in immunosuppressive patients, however, immunocompetent patients may have this condition less frequently. | 2016 | 27546933 |
| do not feed the wildlife: associations between garbage use, aggression, and disease in banded mongooses (mungos mungo). | urbanization and other human modifications of the landscape may indirectly affect disease dynamics by altering host behavior in ways that influence pathogen transmission. few opportunities arise to investigate behaviorally mediated effects of human habitat modification in natural host-pathogen systems, but we provide a potential example of this phenomenon in banded mongooses (mungos mungo), a social mammal. our banded mongoose study population in botswana is endemically infected with a novel myc ... | 2016 | 27547366 |
| new strain multidrug resistant tuberculosis g24767 in puerto rico: old disease a continuous threat. | multidrug resistant tuberculosis (mdr-tb) is defined as a mycobacterium tuberculosis strain resistant to two or more first-line anti-tuberculous drugs. tuberculosis (tb) is a global threat to society despite improvement in therapy as it continues to be an economic burden especially in underdeveloped countries. the downfall of global economics and growing travel destinations in developing countries has escalade the exposure of organism not previously encountered in industrialized nations. most ca ... | 2016 | 27547724 |
| structure of the essential mtb fadd32 enzyme: a promising drug target for treating tuberculosis. | mycolic acids are indispensible lipids of mycobacterium tuberculosis (mtb), the causative agent of tuberculosis (tb), and contribute to the distinctive architecture and impermeability of the mycobacterial cell envelope. fadd32 plays a pivotal role in mycolic acid biosynthesis by functionally linking fatty acid synthase (fas) and polyketide synthase (pks) biosynthetic pathways. fadd32, a fatty acyl-amp ligase (faal), represents one of the best genetically and chemically validated new tb drug targ ... | 2016 | 27547819 |
| plastic-chip-based magnetophoretic immunoassay for point-of-care diagnosis of tuberculosis. | tuberculosis (tb) remains a relevant infectious disease in the 21st century, and its extermination is still far from being attained. due to the extreme infectivity of incipient tb patients, a rapid sensing system for proficient point-of-care (poc) diagnostics is required. in our study, a plastic-chip-based magnetophoretic immunoassay (pcmpi) is introduced using magnetic and gold nanoparticles (nps) modified with mycobacterium tuberculosis (mtb) antibodies. this pcmpi offers an ultrasensitive lim ... | 2016 | 27548010 |
| trisaccharides of phenolic glycolipids confer advantages to pathogenic mycobacteria through manipulation of host-cell pattern-recognition receptors. | despite mycobacterial pathogens continue to be a threat to public health, the mechanisms that allow them to persist by modulating the host immune response are poorly understood. among the factors suspected to play a role are phenolic glycolipids (pgls), produced notably by the major pathogenic species such as mycobacterium tuberculosis and mycobacterium leprae. here, we report an original strategy combining genetic reprogramming of the pgl pathway in mycobacterium bovis bcg and chemical synthesi ... | 2016 | 27548027 |
| direct matrix-assisted laser desorption ionisation time-of-flight mass spectrometry identification of mycobacteria from colonies. | matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (maldi-tof-ms) identification of mycobacteria requires a standard acetonitrile/formic acid pre-maldi-tof-ms. we prospectively compared this standard protocol with direct deposit with matrix for the identification of mycobacteria cultured on solid media. we first verified that mycobacterium tuberculosis was killed after it was mixed with matrix. then, 111 mycobacterium isolates previously identified by partial rpob gene ... | 2016 | 27549109 |
| hiv-1 tat protein vaccination in mice infected with mycobacterium tuberculosis is safe, immunogenic and reduces bacterial lung pathology. | the therapeutic hiv-1 tat protein vaccine is in advanced clinical development. tuberculosis, the main aids co-infection, is highly endemic in areas where aids prevention through vaccination is needed. however, safety and immunogenicity of tat vaccination in the course of mycobacterium tuberculosis (mtb) infection is still unknown and it prevents the possibility to administer the vaccine to mtb-infected individuals. we addressed the interplay and effects of tat vaccination on mtb infection in imm ... | 2016 | 27549342 |
| early bactericidal activity of azd5847 in patients with pulmonary tuberculosis. | azd5847 is an oxazolidinone antibiotic with in vitro activity against mycobacterium tuberculosis the objective of this study was to evaluate the antimycobacterial activity, safety, and pharmacokinetics of azd5847 in patients with pulmonary tuberculosis. groups of 15 treatment-naive, sputum smear-positive adults with pulmonary tuberculosis were randomly assigned to receive azd5847 at one of four doses (500 mg once daily, 500 mg twice daily, 1,200 mg once daily, and 800 mg twice daily) or daily st ... | 2016 | 27550361 |
| impact of lytr-cpsa-psr proteins on cell wall biosynthesis in corynebacterium glutamicum. | proteins of the lcp (lytr, cpsa, psr) family have been shown to inherit important roles in bacterial cell wall biosynthesis. however, their exact function in the formation of the complex cell wall structures of the corynebacteriales, including the prominent pathogens mycobacterium tuberculosis and corynebacterium diphtheriae, remains unclear. here, we analyzed the role of the lcp proteins lcpa and lcpb of corynebacterium glutamicum, both of which localize at regions of nascent cell wall biosynth ... | 2016 | 27551018 |
| hypoxia and classical activation limits mycobacterium tuberculosis survival by akt-dependent glycolytic shift in macrophages. | cellular reactive oxygen species (ros) is a major antibacterial defense mechanism used by macrophages upon activation. exposure of mycobacterium tuberculosis (mtb)-infected macrophages to hypoxia is known to compromise the survival of the pathogen. here we report that the hypoxia-induced control of intracellular mtb load in raw 264.7 macrophages was mediated by regulating the cellular ros levels. we show that similar to classical activation, hypoxia incubation of macrophages resulted in decrease ... | 2016 | 27551515 |
| comparison of maldi-tof ms, nucleic acid hybridization and the mpt64 immunochromatographic test for the identification of m. tuberculosis and non-tuberculosis mycobacterium species. | mycobacteria are an important cause of morbidity in humans. rapid and accurate mycobacterial identification is important for improving patient outcomes. however, identification of mycobacterium species is not easy, due to the slow and fastidious growth of mycobacteria. recently, biochemical, sequencing, and probing methods have come to be used for identification. this study compared the performance of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) for ... | 2016 | 27551724 |
| an easy and sensitive sandwich assay for detection of mycobacterium tuberculosis ag85b antigen using quantum dots and gold nanorods. | mycobacterium tuberculosis is a serious global infectious pathogen causing tuberculosis (tb). the development of an easy and sensitive method for the detection of m. tuberculosis is in urgent need due to complex and low specificity of the current assays. herein, we present a novel method for m. tuberculosis detection based on a sandwich assay via antigen-antibody interaction using silica-coated quantum dots (siqds) and gold nanorods (aunrs). a genetically engineered recombinant antibody (gbp-50b ... | 2017 | 27551994 |
| tuberculosis screening: an update for nps. | one-third of the world's population is infected with mycobacterium tuberculosis. if nps are to effectively participate in eliminating and preventing tuberculosis (tb), they must be cognizant of the current cdc-approved tb screening methods and guidelines. | 2016 | 27552684 |
| the role of prostate apoptosis response-4 (par-4) in mycobacterium tuberculosis infected macrophages. | prostate apoptosis response-4 (par-4) is a tumor suppressor protein that forms a complex with glucose-regulated protein 78 (grp78) to induce apoptosis. previously, we reported that er stress-induced apoptosis is a critical host defense mechanism against mycobacterium tuberculosis (mtb). we sought to understand the role of par-4 during er stress-induced apoptosis in response to mycobacterial infection. par-4 and grp78 protein levels increased in response mtb (strain: h37ra) infection. furthermore ... | 2016 | 27552917 |
| tuberculosis and its management: an overview. | tuberculosis (tb) is a primordial infectious disease that mainly affects the lungs. mycobacterium tuberculosis (m. tuberculosis) is the etiological agent of tb and currently more than one-third of the world population is suffering from tb. for the treatment of tb, administration of multiple antibiotics such as isoniazid, rifampicin, pyrazinamide and ethambutol is required for long time period to kill the bacteria. therefore antibiotic resistance is the emerging problem in multiple drug-resistant ... | 2016 | 27553018 |
| evaluation of multiplex pcr using mpb64 and is6110 primers for rapid diagnosis of tuberculous meningitis. | tuberculous meningitis (tbm) is one of those most serious manifestations of extra-pulmonary tuberculosis and prompt diagnosis and treatment is required for better clinical outcome. it is difficult to diagnose due to lack of rapid, sensitive, and specific tests. newer methods, which are easy and reliable, are required to diagnose tbm at an early stage. thus our aim was to evaluate the multiplex polymerase chain reaction (pcr) technique, using primers directed against the insertion sequence is6110 ... | 2016 | 27553404 |
| comparative study of enzymatic activities of new katg mutants from low- and high-level isoniazid-resistant clinical isolates of mycobacterium tuberculosis. | resistance to isoniazid (inh-r) in mycobacterium tuberculosis is mainly due to mutations at position 315 (s315t) of the catalase-peroxidase katg. we identified 16 mutations (including 13 biochemically uncharacterized mutations) in katg from inh-r clinical isolates of m. tuberculosis showing mutations other than s315t. the katg enzymatic activities (catalase, peroxidase, free radical production and isonicotinoyl-nad formation) of wild-type katg and the 16 mutants were determined and correlated to ... | 2016 | 27553406 |
| mmpl transporter-mediated export of cell-wall associated lipids and siderophores in mycobacteria. | mycobacteria produce a large variety of surface-exposed lipids with unusual structures. some of these compounds are ubiquitously present in mycobacteria and play an important role in the structural organization of the cell envelope, while others are species-specific. the biosynthesis of most of these lipids requires modular polyketide synthases (pks) or non-ribosomal peptide synthetases (nrps) that are intracellular, suggesting that the assembly of these compounds takes place in the cytosolic co ... | 2016 | 27553408 |
| a snapshot of the predominant single nucleotide polymorphism cluster groups of mycobacterium tuberculosis clinical isolates in delhi, india. | several attempts have been made to associate phylogenetic differences among mycobacterium tuberculosis strains to variations in the clinical outcome of the disease and to drug resistance. we genotyped 139 clinical isolates of m. tuberculosis obtained from patients of pulmonary tuberculosis in north delhi region. the isolates were analyzed using nine single nucleotide polymorphism (snp) markers, spoligotyping and miru-vntrs; and the results were correlated with their drug susceptibility profile. ... | 2016 | 27553413 |
| immunoglobulin g response to mammalian cell entry 1a (mce1a) protein as biomarker of active tuberculosis. | cell wall components are major determinants of virulence of mycobacterium tuberculosis and they contribute to the induction of both humoral and cell-mediated immune response. the mammalian cell entry protein 1a (mce1a), in the cell wall of m. tuberculosis, mediates entry of the pathogen into mammalian cells. here, we examined serum immunoglobulin levels (iga, igm and total igg) against mce1a as a potential biomarker for diagnosis and monitoring tuberculosis (tb) treatment response. serum samples ... | 2016 | 27553414 |
| sputum is a surrogate for bronchoalveolar lavage for monitoring mycobacterium tuberculosis transcriptional profiles in tb patients. | pathogen-targeted transcriptional profiling in human sputum may elucidate the physiologic state of mycobacterium tuberculosis (m. tuberculosis) during infection and treatment. however, whether m. tuberculosis transcription in sputum recapitulates transcription in the lung is uncertain. we therefore compared m. tuberculosis transcription in human sputum and bronchoalveolar lavage (bal) samples from 11 hiv-negative south african patients with pulmonary tuberculosis. we additionally compared these ... | 2016 | 27553415 |
| micrococcin p1 - a bactericidal thiopeptide active against mycobacterium tuberculosis. | the lack of proper treatment for serious infectious diseases due to the emergence of multidrug resistance reinforces the need for the discovery of novel antibiotics. this is particularly true for tuberculosis (tb) for which 3.7% of new cases and 20% of previously treated cases are estimated to be caused by multi-drug resistant strains. in addition, in the case of tb, which claimed 1.5 million lives in 2014, the treatment of the least complicated, drug sensitive cases is lengthy and disagreeable. ... | 2016 | 27553416 |
| dissecting host factors that regulate the early stages of tuberculosis infection. | incomplete understanding of mechanisms involved in the host-pathogen interactions constrains our efforts to eliminate tuberculosis. in many individuals, resulting from immune response to mycobacterial infection organised structures called granulomas are formed. to identify host responses that may control at least the early stages of infection, we employed an in vitro granuloma model. here, human pbmcs were infected with live mycobacterium tuberculosis in culture, and the appearance of granuloma- ... | 2016 | 27553417 |
| loop-mediated isothermal amplification (lamp) assay for speedy diagnosis of tubercular lymphadenitis: the multi-targeted 60-minute approach. | tuberculous lymphadenitis (tbla), the most common presentation of tuberculosis, poses a significant diagnostic challenge in the developing countries. timely, accurate and cost-effective diagnosis can decrease the high morbidity associated with tbla especially in resource-poor high-endemic regions. the loop-mediated isothermal amplification assay (lamp), using two targets, was evaluated for the diagnosis of tbla. | 2016 | 27553418 |
| safety and immunogenicity of the m72/as01e candidate tuberculosis vaccine in adults with tuberculosis: a phase ii randomised study. | previous studies have shown that the m72/as01e candidate tuberculosis vaccine is immunogenic with a clinically acceptable safety profile in healthy and mycobacterium tuberculosis-infected adults. this phase ii, observer-blind, randomised study compared the safety, reactogenicity, and immunogenicity of m72/as01e in 3 cohorts: tuberculosis-naïve adults (n = 80), adults previously treated for tuberculosis (n = 49), and adults who have completed the intensive phase of tuberculosis treatment (n = 13) ... | 2016 | 27553419 |
| corrigendum to "katg ser315 and rpob 81-bp hotspot region substitutions: reliability for detection of drug-resistant strains of mycobacterium tuberculosis" [j. global antimicrob. resist. 5 (2016) 92-93]. | 2016 | 27553705 | |
| plasma contributes to the antimicrobial activity of whole blood against mycobacterium tuberculosis. | the whole blood model for infection has proven useful to analyze the immunological response to mycobacterium tuberculosis, because it exerts a significant antimicrobial activity. although this activity has been generally assumed to be cellular, we have found that the leukocyte fraction of blood from healthy volunteers did not kill the bacilli. we have discovered that plasma was responsible for a large proportion, but not all, of the antimicrobial activity. furthermore, infected monocytes control ... | 2016 | 27554054 |
| subunit vaccine h56/caf01 induces a population of circulating cd4 t cells that traffic into the mycobacterium tuberculosis-infected lung. | the capacity of cd4 t cells to protect against mycobacterium tuberculosis (mtb) is governed by their ability to localize to the lung site of infection. subunit vaccine h56/caf01, a liposome-adjuvanted fusion protein of mtb antigens ag85b, esat-6, and rv2660, conferred durable protection and elicited polyfunctional cd4 t cells that preferentially localized to the lung parenchyma. these lung-resident t cells had reduced klrg1 and increased cxcr3 expression, an intermediate state of th1 differentia ... | 2017 | 27554293 |
| human cd40 ligand deficiency dysregulates the macrophage transcriptome causing functional defects that are improved by exogenous ifn-γ. | cd40 ligand (cd40l) deficiency predisposes to opportunistic infections, including those caused by fungi and intracellular bacteria. studies of cd40l-deficient patients reveal the critical role of cd40l-cd40 interaction for the function of t, b, and dendritic cells. however, the consequences of cd40l deficiency on macrophage function remain to be investigated. | 2017 | 27554817 |
| a rheostat mechanism governs the bifurcation of carbon flux in mycobacteria. | fatty acid metabolism is an important feature of the pathogenicity of mycobacterium tuberculosis during infection. consumption of fatty acids requires regulation of carbon flux bifurcation between the oxidative tca cycle and the glyoxylate shunt. in escherichia coli, flux bifurcation is regulated by phosphorylation-mediated inhibition of isocitrate dehydrogenase (icd), a paradigmatic example of post-translational mechanisms governing metabolic fluxes. here, we demonstrate that, in contrast to e. ... | 2016 | 27555519 |
| whole genome sequencing of mycobacterium tuberculosis sb24 isolated from sabah, malaysia. | mycobacterium tuberculosis (m. tuberculosis) is the causative agent of tuberculosis (tb) that causes millions of death every year. we have sequenced the genome of m. tuberculosis isolated from cerebrospinal fluid (csf) of a patient diagnosed with tuberculous meningitis (tbm). the isolated strain was referred as m. tuberculosis sb24. genomic dna of the m. tuberculosis sb24 was extracted and subjected to whole genome sequencing using pacbio platform. the draft genome size of m. tuberculosis sb24 w ... | 2016 | 27556011 |
| chemical insight into the mechanism and specificity of glft2, a bifunctional galactofuranosyltransferase from mycobacteria. | mycobacteria, including the human pathogen mycobacterium tuberculosis, produce a complex cell wall structure that is essential to survival. a key component of this structure is a glycoconjugate, the mycolyl-arabinogalactan-peptidoglycan complex, which has at its core a galactan domain composed of galactofuranose (galf) residues linked to peptidoglycan. because galactan biosynthesis is essential for mycobacterial viability, compounds that interfere with this process are potential therapeutic agen ... | 2016 | 27557056 |
| mycobacterium tuberculosis phosphate uptake system component psta2 is not required for gene regulation or virulence. | the mycobacterium tuberculosis genome encodes two complete high-affinity pst phosphate-specific transporters. we previously demonstrated that a membrane-spanning component of one pst system, psta1, was essential both for m. tuberculosis virulence and for regulation of gene expression in response to external phosphate availability. to determine if the alternative pst system is similarly required for virulence or gene regulation, we constructed a deletion of psta2. transcriptome analysis revealed ... | 2016 | 27557082 |
| investments in tuberculosis research - what are the gaps? | through decades of research, numerous studies have generated robust evidence about effective interventions for tuberculosis control. yet, the global annual decline in incidence of approximately 1 % is evidence that current approaches and investment strategies are not sufficient. in this article, we assess recent tuberculosis research funding and discuss two critical gaps in funding and in scientific evidence from topics that have been left off the research priority agenda.we first examine resear ... | 2016 | 27558175 |
| deoxyfluoro-d-trehalose (fdtre) analogues as potential pet probes for imaging mycobacterial infection. | mycobacterium tuberculosis, the etiological agent of human tuberculosis, requires the non-mammalian disaccharide trehalose for growth and virulence. recently, detectable trehalose analogues have gained attention as probes for studying trehalose metabolism and as potential diagnostic imaging agents for mycobacterial infections. of particular interest are deoxy-[(18)f]fluoro-d-trehalose ((18)f-fdtre) analogues, which have been suggested as possible positron emission tomography (pet) probes for in ... | 2016 | 27560008 |
| antimycobacterial metabolites from marine invertebrates. | marine organisms play an important role in natural product-based drug research due to accumulation of structurally unique and bioactive metabolites. the exploration of marine-derived compounds may significantly extend the scientific knowledge of potential scaffolds for antibiotic drug discovery. development of novel antitubercular agents is especially significant as the emergence of drug-resistant mycobacterium tuberculosis strains remains threateningly high. marine invertebrates (i.e., sponges, ... | 2016 | 27561360 |
| suppression of autophagy and antigen presentation by mycobacterium tuberculosis pe_pgrs47. | suppression of major histocompatibility complex (mhc) class ii antigen presentation is believed to be among the major mechanisms used by mycobacterium tuberculosis to escape protective host immune responses. through a genome-wide screen for the genetic loci of m. tuberculosis that inhibit mhc class ii-restricted antigen presentation by mycobacteria-infected dendritic cells, we identified the pe_pgrs47 protein as one of the responsible factors. targeted disruption of the pe_pgrs47 (rv2741) gene l ... | 2016 | 27562263 |
| a subunit vaccine based on rh-ns induces protection against mycobacterium tuberculosis infection by inducing the th1 immune response and activating macrophages. | mycobacterium tuberculosis (mtb) is a gram-positive pathogen which causes tuberculosis in both animals and humans. all tested rh-ns formulations induced a specific th1 response, as indicated by increased production of interferon γ (ifn-γ) and interleukin 2 (il-2) by lymphocytes in the spleen of mice which were immunized with rh-ns alone or with rh-ns and the adjuvant cyclic gmp-amp (cgamp). serum from mice immunized with rh-ns with or without adjuvant also had higher levels of il-12p40 and tnf-α ... | 2016 | 27563010 |
| synthesis and antitubercular activity of new benzo[b]thiophenes. | in vitro and ex vivo efficacies of four series of benzo[b]thiophene-2-carboxylic acid derivatives were studied against mycobacterium tuberculosis h37ra (mtb). benzo[b]thiophenes were also tested in vitro against multidrug resistant mycobacterium tuberculosis h37ra (mdr-mtb), and 7b was found to be highly active against a- and d-mdr-mtb/mtb (mic ranges 2.73-22.86 μg/ml). the activity of all benzo[b]thiophenes against m. bovis bcg (bcg) was also assessed grown under aerobic and under conditions of ... | 2016 | 27563398 |
| spatio-temporal distribution of mycobacterium tuberculosis complex strains in ghana. | there is a perception that genomic differences in the species/lineages of the nine species making the mycobacterium tuberculosis complex (mtbc) may affect the efficacy of distinct control tools in certain geographical areas. we therefore analyzed the prevalence and spatial distribution of mtbc species and lineages among isolates from pulmonary tb cases over an 8-year period, 2007-2014. | 2016 | 27564240 |
| reconstitution of protein translation of mycobacterium reveals functional conservation and divergence with the gram-negative bacterium escherichia coli. | protein translation is essential for all bacteria pathogens. it has also been a major focus of structural and functional studies and an important target of antibiotics. here we report our attempts to biochemically reconstitute mycobacterial protein translation in vitro from purified components. this mycobacterial translation system consists of individually purified recombinant translation factors from mycobacterium tuberculosis (m. tuberculosis), purified trnas and ribosomes from mycobacterium s ... | 2016 | 27564552 |
| the essential mycobacterial amidotransferase gatcab is a modulator of specific translational fidelity. | although regulation of translation fidelity is an essential process(1-7), diverse organisms and organelles have differing requirements of translational accuracy(8-15), and errors in gene translation serve an adaptive function under certain conditions(16-20). therefore, optimal levels of fidelity may vary according to context. most bacteria utilize a two-step pathway for the specific synthesis of aminoacylated glutamine and/or asparagine trnas, involving the glutamine amidotransferase gatcab(21-2 ... | 2016 | 27564922 |
| post-translational acetylation of mbta modulates mycobacterial siderophore biosynthesis. | iron is an essential element for life, but its soluble form is scarce in the environment and is rarer in the human body. mtb (mycobacterium tuberculosis) produces two aryl-capped siderophores, mycobactin (mbt) and carboxymycobactin (cmbt), to chelate intracellular iron. the adenylating enzyme mbta catalyzes the first step of mycobactin biosynthesis in two half-reactions: activation of the salicylic acid as an acyl-adenylate and ligation onto the acyl carrier protein (acp) domain of mbtb to form ... | 2016 | 27566542 |
| mycobacterium tuberculosis multistage antigens confer comprehensive protection against pre- and post-exposure infections by driving th1-type t cell immunity. | there is an urgent need for a vaccine against tuberculosis (tb) that is more effective than the current sole licensed option. however, target antigens of mycobacterium tuberculosis with the vaccine potential remain elusive. five immunodominant antigens with characteristic expressions at the stages of primary infection (ag85a), the regulation of nutrition and metabolism when transferring from rapid growth to latency (phoy2 and rv3407), latency (rv2626c), and reactivation (rpfb) were selected to c ... | 2016 | 27566581 |
| drug-resistant tuberculosis viewed from bacterial and host genomes. | the outcome of infection with mycobacterium tuberculosis (mtb) is largely influenced by the host-pathogen interaction in which both the human host and the mtb genetic backgrounds play an important role. whether this interaction also influences the selection and expansion of drug-resistant mtb strains is the primary focus of this review. we first outline the main and recent findings regarding mtb determinants implicated in the development of drug resistance. second, we examine data regarding huma ... | 2016 | 27566907 |
| identification of mycobacterium spp. and nocardia spp. from solid and liquid cultures by matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms). | identification of microorganisms by maldi-tof ms has been widely accepted in clinical microbiology. however, for mycobacterium spp. and nocardia spp. such identification has not yet reached the optimal level of routine testing. here we describe the development of an identification tool for 49 and 15 species of mycobacterium spp. and nocardia spp., respectively. during database construction, a number of ambiguous reference identifications were revealed and corrected via molecular analyses. eventu ... | 2016 | 27567285 |
| mycobacterium tuberculosis lppm displays an original structure and domain composition linked to a dual localization. | mycobacterium tuberculosis (mtb) encodes several bacterial effectors impacting the colonization of phagocytes. lppm (rv2171) is both implicated in phagocytosis and able to efficiently block phagosomal acidification in the macrophage, two key processes contributing to mtb persistence. we show that lppm is anchored to the mycobacterial cell wall by a c-terminal membrane domain. however, the protein also exists as a truncated protein secreted into the culture medium. the lppm solution structure we ... | 2016 | 27568926 |
| mycobacterium tuberculosis (mtb) gyrb inhibitors: an attractive approach for developing novel drugs against tb. | new classes of drugs are needed to treat tuberculosis (tb) in order to combat the emergence of resistance (mdr and xdr) to existing agents and shorten the duration of therapy. mycobacterial dna gyrase b subunit has been identified to be one of the potentially under exploited drug targets in the field of antitubercular drug discovery. in the present review, we discussed the synthesis, structural optimization and docking study of effective potent dna gyrase inhibitor against m. tuberculosis, with ... | 2016 | 27569197 |
| enolase of mycobacterium tuberculosis is a surface exposed plasminogen binding protein. | enolase, a glycolytic enzyme, has long been studied as an anchorless protein present on the surface of many pathogenic bacteria that aids in tissue remodeling and invasion by binding to host plasminogen. | 2017 | 27569900 |
| mycobacterium aurum is unable to survive mycobacterium tuberculosis latency associated stress conditions: implications as non-suitable model organism. | mycobacterium tuberculosis manages to remain latent in the human body regardless of extensive chemotherapy. complete eradication of tuberculosis (tb) requires treatment strategies targeted against latent form of infection, in addition to the current regimen of antimycobacterials. many in vitro and in vivo models have been proposed to imitate latent tb infection, yet none of them is able to completely mimic latent infection state of m. tuberculosis. highly infectious nature of the pathogen requir ... | 2016 | 27570312 |
| two-year follow-up study of mycobacterium tuberculosis antigen-driven ifn-γ responses and macrophage scd14 levels after tuberculosis contact. | clinical data regarding the prediction of active tuberculosis (tb) development in close tb contacts are scarce. to address this problem, we performed a 2-year follow-up study of mycobacterium tuberculosis (m.tb) antigen-driven ifn-gamma responses and serum levels of soluble macrophage cd14 receptor in individuals with recent or prolonged m.tb exposure. between june 2011 and june 2013, we studied 60 healthy polish adults with recent household or long-term work tb contact and individuals without k ... | 2016 | 27570313 |
| acute respiratory distress syndrome complicating community-acquired pneumonia secondary to mycobacterium tuberculosis in a tertiary care center in saudi arabia. | to discuss our center's experience with acute respiratory distress syndrome (ards) secondary to pulmonary tuberculosis (tb) in a major tertiary referral hospital in the kingdom of saudi arabia. | 2016 | 27570853 |
| blockade of the kv1.3 k+ channel enhances bcg vaccine efficacy by expanding central memory t lymphocytes. | tuberculosis is the oldest known infectious disease, yet there is no effective vaccine against adult pulmonary tuberculosis. emerging evidence indicates that t-helper 1 and t-helper 17 cells play important roles in host protection against tuberculosis. however, tuberculosis vaccine efficacy in mice is critically dependent on the balance between antigen-specific central memory t (tcm) and effector memory t (tem) cells. specifically, a high tcm/tem cell ratio is essential for optimal vaccine effic ... | 2016 | 27571906 |
| thpp target assignment reveals echa6 as an essential fatty acid shuttle in mycobacteria. | phenotypic screens for bactericidal compounds against drug-resistant tuberculosis are beginning to yield novel inhibitors. however, reliable target identification remains challenging. here, we show that tetrahydropyrazo[1,5-a]pyrimidine-3-carboxamide (thpp) selectively pulls down echa6 in a stereospecific manner, instead of the previously assigned target mycobacterium tuberculosis mmpl3. while homologous to mammalian enoyl-coenzyme a (coa) hydratases, echa6 is non-catalytic yet essential and bin ... | 2016 | 27571973 |
| pks5-recombination-mediated surface remodelling in mycobacterium tuberculosis emergence. | mycobacterium tuberculosis is a major, globally spread, aerosol-transmitted human pathogen, thought to have evolved by clonal expansion from a mycobacterium canettii-like progenitor. in contrast, extant m. canettii strains are rare, genetically diverse, and geographically restricted mycobacteria of only marginal epidemiological importance. here, we show that the contrasting evolutionary success of these two groups is linked to loss of lipooligosaccharide biosynthesis and subsequent morphotype ch ... | 2016 | 27571976 |
| genomic analysis of the evolution of fluoroquinolone resistance in mycobacterium tuberculosis prior to tuberculosis diagnosis. | fluoroquinolones (fqs) are effective second-line drugs for treating antibiotic-resistant tuberculosis (tb) and are being considered for use as first-line agents. because fqs are used to treat a range of infections, in a setting of undiagnosed tb, there is potential to select for drug-resistant mycobacterium tuberculosis mutants during fq-based treatment of other infections, including pneumonia. here we present a detailed characterization of ofloxacin-resistant m. tuberculosis samples isolated di ... | 2016 | 27572408 |
| bioluminescent reporters for rapid mechanism of action assessment in tuberculosis drug discovery. | the tuberculosis (tb) drug discovery pipeline is fueled by compounds identified in whole-cell screens against the causative agent, mycobacterium tuberculosis phenotypic screening enables the selection of molecules that inhibit essential cellular functions in live, intact bacilli grown under a chosen in vitro condition. however, deducing the mechanism of action (moa), which is important to avoid promiscuous targets, often requires significant biological resources in a lengthy process that risks d ... | 2016 | 27572410 |
| role of cathepsins in mycobacterium tuberculosis survival in human macrophages. | cathepsins are proteolytic enzymes that function in the endocytic pathway, especially in lysosomes, where they contribute directly to pathogen killing or indirectly, by their involvement in the antigen presentation pathways. mycobacterium tuberculosis (mtb) is a facultative intracellular pathogen that survives inside the macrophage phagosomes by inhibiting their maturation to phagolysosomes and thus avoiding a low ph and protease-rich environment. we previously showed that mycobacterial inhibiti ... | 2016 | 27572605 |
| identifying lineage effects when controlling for population structure improves power in bacterial association studies. | bacteria pose unique challenges for genome-wide association studies because of strong structuring into distinct strains and substantial linkage disequilibrium across the genome(1,2). although methods developed for human studies can correct for strain structure(3,4), this risks considerable loss-of-power because genetic differences between strains often contribute substantial phenotypic variability(5). here, we propose a new method that captures lineage-level associations even when locus-specific ... | 2016 | 27572646 |
| structural insight into dephosphorylation by trehalose 6-phosphate phosphatase (otsb2) from mycobacterium tuberculosis. | trehalose serves as a key structural component in the cell wall of mycobacterium tuberculosis. m. tuberculosis trehalose-6-phosphate phosphatase (mtbtpp), an essential enzyme in the trehalose biosynthesis otsab pathway, catalyzes the dephosphorylation of trehalose-6-phosphate (trehalose-6-p) to generate trehalose, and plays a critical role in m. tuberculosis survival-associated cell wall formation and permeability. therefore, mtbtpp (otsb2) is considered a promising potential target for discover ... | 2016 | 27572957 |
| network analysis identifies rv0324 and rv0880 as regulators of bedaquiline tolerance in mycobacterium tuberculosis. | the resilience of mycobacterium tuberculosis (mtb) emerges from its ability to effectively counteract immunological, environmental and antitubercular challenges. here, we demonstrate that mtb can tolerate drug treatment by adopting a tolerant state that can be deciphered through systems analysis of its transcriptional responses. specifically, we demonstrate how treatment with the antitubercular drug bedaquiline activates a regulatory network that coordinates multiple resistance mechanisms to pus ... | 2016 | 27573104 |
| plasma adenosine deaminase enzyme reduces with treatment of pulmonary tuberculosis in nigerian patients: indication for diagnosis and treatment monitoring. | tuberculosis(tb)-specific host biomarkers for diagnosis and monitoring of treatment response have been identified as priorities for tb research. macrophage and t cell lymphocytes play vital roles in mycobacterium tuberculosis immune response and their associated biomarkers could form good candidates for diagnosis and treatment monitoring. the enzyme adenosine deaminase (ada) is produced mainly by monocytes and macrophages and increase in biological fluids in the course of infection with microorg ... | 2016 | 27574764 |
| parallel reaction monitoring of clinical mycobacterium tuberculosis lineages reveals pre-existent markers of rifampicin tolerance in the emerging beijing lineage. | the spread of multidrug resistant mycobacterium tuberculosis is one of the major challenges in tuberculosis control. in eurasia, the spread of multidrug resistant tuberculosis is driven by the m. tuberculosis beijing genotype. in this study, we examined whether selective advantages are present in the proteome of beijing isolates that contribute to the emergence of this genotype. to this end, we compared the proteome of m. tuberculosis beijing to that of m. tuberculosis h37rv, both in the presenc ... | 2017 | 27576137 |
| crystal structure of vapc21 from mycobacterium tuberculosis at 1.31 å resolution. | persisters are individual bacterial cells that exhibit a phenotype characterized by slow growth, low metabolic rate and multidrug tolerance. the processes that drive cells into a persistence state constitute an active but incipient research field, and structural data regarding its components are scarce. the molecular targets of many therapeutic drugs are involved in cell wall synthesis and cell division, and these cellular processes are down-regulated in persister cells, consequently these cells ... | 2016 | 27576202 |
| effect of peg and water-soluble chitosan coating on moxifloxacin-loaded plga long-circulating nanoparticles. | moxifloxacin (mox) is a mycobacterium tuberculosis dna gyrase inhibitor. due to its intense hydrophilicity, mox is cleared from the body within 24 h and required for repetitive doses which may then result in hepatotoxicity and acquisition of mox resistant-tb, related with its use. to overcome the aforementioned limitations, the current study aimed to develop plga nanoparticles (plga nps), to act as an efficient carrier for controlled delivery of mox. to achieve a substantial extension in blood c ... | 2017 | 27576453 |
| shedding light on the performance of a pyrosequencing assay for drug-resistant tuberculosis diagnosis. | rapid molecular diagnostics, with their ability to quickly identify genetic mutations associated with drug resistance in mycobacterium tuberculosis clinical specimens, have great potential as tools to control multi- and extensively drug-resistant tuberculosis (m/xdr-tb). the qiagen pyromark q96 id system is a commercially available pyrosequencing (psq) platform that has been validated for rapid m/xdr-tb diagnosis. however, the details of the assay's diagnostic and technical performance have yet ... | 2016 | 27576542 |
| modulation of the immune response to mycobacterium tuberculosis during malaria/m. tuberculosis co-infection. | tuberculosis (tb) causes significant morbidity and mortality on a global scale. the african region has 24% of the world's tb cases. tb overlaps with other infectious diseases such as malaria and hiv, which are also highly prevalent in the african region. tb is a leading cause of death among hiv-positive patients and co-infection with hiv and tb has been described as a syndemic. in view of the overlapping epidemiology of these diseases, it is important to understand the dynamics of the immune res ... | 2017 | 27577087 |
| antibiotic bactericidal activity is countered by maintaining ph homeostasis in mycobacterium smegmatis. | antibiotics target specific biosynthetic processes essential for bacterial growth. it is intriguing that several commonalities connect the bactericidal activity of seemingly disparate antibiotics, such as the numerous conditions that confer broad-spectrum antibiotic tolerance. whether antibiotics kill in a manner unique to their specific targets or by a universal mechanism is a critical and contested subject. herein, we demonstrate that the bactericidal activity of diverse antibiotics against my ... | 2017 | 27579369 |
| propargyl-linked antifolates are potent inhibitors of drug-sensitive and drug-resistant mycobacterium tuberculosis. | mycobacterium tuberculosis continues to cause widespread, life-threatening disease. in the last decade, this threat has grown dramatically as multi- and extensively-drug resistant (mdr and xdr) bacteria have spread globally and the number of agents that effectively treat these infections is significantly reduced. we have been developing the propargyl-linked antifolates (plas) as potent inhibitors of the essential enzyme dihydrofolate reductase (dhfr) from bacteria and recently found that charged ... | 2016 | 27580226 |
| ifn-γ release assay result is associated with disease site and death in active tuberculosis. | the ifn-γ release assays and tuberculin skin tests are used to support the diagnosis of both latent and active tuberculosis. however, we previously demonstrated that a negative tuberculin test in active tuberculosis is associated with disseminated disease and death. it is unknown whether the same associations exist for ifn-γ release assays. | 2016 | 27580246 |
| identification of kasa as the cellular target of an anti-tubercular scaffold. | phenotypic screens for bactericidal compounds are starting to yield promising hits against tuberculosis. in this regard, whole-genome sequencing of spontaneous resistant mutants generated against an indazole sulfonamide (gsk3011724a) identifies several specific single-nucleotide polymorphisms in the essential mycobacterium tuberculosis β-ketoacyl synthase (kas) a gene. here, this genomic-based target assignment is confirmed by biochemical assays, chemical proteomics and structural resolution of ... | 2016 | 27581223 |
| smear positive pulmonary tuberculosis and associated factors among homeless individuals in dessie and debre birhan towns, northeast ethiopia. | tuberculosis (tb) remains one of the globe's deadliest communicable diseases. the homeless individuals are at high risk to acquire tb and multi-drug resistant tb (mdr-tb), because of their poor living conditions and risky behaviors. tuberculosis and mdr-tb in the homeless individuals can pose a risk to entire communities. however, the magnitude of the problem is not known in ethiopia. therefore, the aim of this study was to determine the prevalence and associated factors of smear positive pulmon ... | 2016 | 27581729 |
| insights into horizontal acquisition patterns of dormancy and reactivation regulon genes in mycobacterial species using a partitioning-based framework. | horizontal gene transfer (hgt) events, initially thought to be rare in mycobacterium tuberculosis, have recently been shown to be involved in the acquisition of virulence operons in m. tuberculosis. we have developed a new partitioning framework based hgt prediction algorithm, called grid3m, and applied the same for the prediction of hgts in mycobacteria. validation and testing using simulated and real microbial genomes indicated better performance of grid3m as compared with other widely used hg ... | 2016 | 27581938 |