Publications

TitleAbstractYear
Filter
PMID(sorted ascending)
Filter
synthesis, 3d-qsar analysis and biological evaluation of quinoxaline 1,4-di-n-oxide derivatives as antituberculosis agents.a series of quinoxaline 1,4-di-n-oxide derivatives variously substituted at c-2 position were synthesized and evaluated for in vitro antimycobacterial activity. seventeen compounds exhibited potential activity (mic ⩽6.25μg/ml) against mycobacterium tuberculosis (h37rv), in particular the compounds 3d and 3j having an mic value of 0.39μg/ml. none of the compounds exhibited cytotoxicity when using an mtt assay in vero cells. to further investigate the structure-activity relationship, comfa (q(2)=0 ...201627426298
polymorphisms of 20 regulatory proteins between mycobacterium tuberculosis and mycobacterium bovis.mycobacterium tuberculosis and mycobacterium bovis are responsible for tuberculosis in humans and animals, respectively. both species are closely related and belong to the mycobacterium tuberculosis complex (mtc). m. tuberculosis is the most ancient species from which m. bovis and other members of the mtc evolved. the genome of m. bovis is over >99.95% identical to that of m. tuberculosis but with seven deletions ranging in size from 1 to 12.7 kb. in addition, 1200 single nucleotide mutations in ...201627427512
antitubercular drugs for an old target: gsk693 as a promising inha direct inhibitor.despite being one of the first antitubercular agents identified, isoniazid (inh) is still the most prescribed drug for prophylaxis and tuberculosis (tb) treatment and, together with rifampicin, the pillars of current chemotherapy. a high percentage of isoniazid resistance is linked to mutations in the pro-drug activating enzyme katg, so the discovery of direct inhibitors (di) of the enoyl-acp reductase (inha) has been pursued by many groups leading to the identification of different enzyme inhib ...201627428438
hif-1α is an essential mediator of ifn-γ-dependent immunity to mycobacterium tuberculosis.the cytokine ifn-γ coordinates macrophage activation and is essential for control of pathogens, including mycobacterium tuberculosis however, the mechanisms by which ifn-γ controls m. tuberculosis infection are only partially understood. in this study, we show that the transcription factor hypoxia-inducible factor-1α (hif-1α) is an essential mediator of ifn-γ-dependent control of m. tuberculosis infection both in vitro and in vivo. m. tuberculosis infection of ifn-γ-activated macrophages results ...201627430718
[analysis of koch phenomenon of mycobacterium tuberculosis-infected guinea pigs vaccinated with recombinant tuberculosis vaccine aec/bc02].to observe the koch phenomenon of mycobacterium tuberculosis(mtb)-infected guinea pigs after vaccinated with killed h37ra bacteria or tuberculosis vaccine candidate aec/bc02.201627430923
[experimental study of genexpert(®) system in the diagnosis of extra-pulmonary tuberculosis].to explore the application value of genexpert mtb/rif for detection of extra-pulmonary tuberculosis and resistance to rifampin.201627430924
8-hydroxyquinolines are boosting agents of copper-related toxicity in mycobacterium tuberculosis.copper (cu) ions are likely the most important immunological metal-related toxin utilized in controlling bacterial infections. impairment of bacterial cu resistance reduces viability within the host. thus, pharmacological enhancement of cu-mediated antibacterial toxicity may lead to novel strategies in drug discovery and development. screening for cu toxicity-enhancing antibacterial molecules identified 8-hydroxyquinoline (8hq) to be a potent cu-dependent bactericidal inhibitor of mycobacterium ...201627431227
two new bioactive iridoids from rothmannia wittii.the first reported study of the isolation and identification of compounds from the bark and fruit of rothmannia wittii yielded two new iridoids, 6β-hydroxy-10-o-acetylgenipin (1) and 10-o-acetylmacrophyllide (2) together with six known iridoids; 6β-hydroxygenipin (3), genipin (4), garjasmine (5), cerbinal (6), and mixture of β-gardiol (7) and α-gardiol (8); benzoic acid (9); vanillic acid (10); and stigmasterol (11). their structures were elucidated by spectroscopic methods. iridoid 1 showed ant ...201627431771
successful treatment of peritoneal dialysis-related peritonitis due to mycobacterium iranicum.a 68-year-old man on peritoneal dialysis (pd) was hospitalized with the clinical picture of peritonitis. the patient was diagnosed with peritonitis caused by nontuberculous mycobacteria (ntm) according to positive ziehl-neelsen staining and negative mycobacterium tuberculosis polymerase chain reaction results. oral levofloxacin and clarithromycin, and later intraperitoneal imipenem were started. according to the anti-ntm susceptibility test results, oral minocycline was administered. the patient ...201627432106
a potential target gene for the host-directed therapy of mycobacterial infection in murine macrophages.mycobacterium tuberculosis (mtb), one of the major bacterial pathogens for lethal infectious diseases, is capable of surviving within the phagosomes of host alveolar macrophages; therefore, host genetic variations may alter the susceptibility to mtb. in this study, to identify host genes exploited by mtb during infection, genes were non-selectively inactivated using lentivirus-based antisense rna methods in raw264.7 macrophages, and the cells that survived virulent mtb infection were then screen ...201627432120
infectious complications with the use of biologic response modifiers in infants and children.biologic response modifiers (brms) are substances that interact with and modify the host immune system. brms that dampen the immune system are used to treat conditions such as juvenile idiopathic arthritis, psoriatic arthritis, or inflammatory bowel disease and often in combination with other immunosuppressive agents, such as methotrexate and corticosteroids. cytokines that are targeted include tumor necrosis factor α; interleukins (ils) 6, 12, and 23; and the receptors for il-1α (il-1a) and il- ...201627432853
n-methylation of a bactericidal compound as a resistance mechanism in mycobacterium tuberculosis.the rising incidence of antimicrobial resistance (amr) makes it imperative to understand the underlying mechanisms. mycobacterium tuberculosis (mtb) is the single leading cause of death from a bacterial pathogen and estimated to be the leading cause of death from amr. a pyrido-benzimidazole, 14, was reported to have potent bactericidal activity against mtb. here, we isolated multiple mtb clones resistant to 14. each had mutations in the putative dna-binding and dimerization domains of rv2887, a ...201627432954
pulmonary tuberculosis diagnosis: where we are?in recent years, in spite of medical advancement, tuberculosis (tb) remains a worldwide health problem. although many laboratory methods have been developed to expedite the diagnosis of tb, delays in diagnosis remain a major problem in the clinical practice. because of the slow growth rate of the causative agent mycobacterium tuberculosis, isolation, identification, and drug susceptibility testing of this organism and other clinically important mycobacteria can take several weeks or longer. duri ...201627433173
determination of urinary neopterin/creatinine ratio to distinguish active tuberculosis from latent mycobacterium tuberculosis infection.background. biomarkers to distinguish latent from active mycobacterium (m.) tuberculosis infection in clinical practice are lacking. the urinary neopterin/creatinine ratio can quantify the systemic interferon-gamma effect in patients with m. tuberculosis infection. methods. in a prospective observational study, urinary neopterin levels were measured by enzyme linked immunosorbent assay in patients with active tuberculosis, in people with latent m. tuberculosis infection, and in healthy controls ...201627433370
a computational framework for tracing the origins of genomic islands in prokaryotes.genomic islands (gis) are chunks of genomic fragments that are acquired from nongenealogical organisms through horizontal gene transfer (hgt). current researches on studying donor-recipient relationships for hgt are limited at a gene level. as more gis have been identified and verified, the way of studying donor-recipient relationships can be better modeled by using gis rather than individual genes. in this paper, we report the development of a computational framework for detecting origins of gi ...201427433520
two infants with tuberculid associated with kawasaki disease.bacille de calmette et guerin (bcg) is the only licensed tuberculosis vaccine to prevent severe tuberculosis. the adverse events of bcg vaccination, including local reactions, lymphadenitis, osteomyelitis, tuberculid, and disseminated infection, have been reported. two infants presented erythema at the inoculation site of bcg after the resolution of kawasaki disease (kd). they received bcg vaccination 1 week and 6 weeks before the kd onset, respectively. intravenous immunoglobulin improved the k ...201627435523
mutations in rrs, rpsl and gidb in streptomycin-resistant mycobacterium tuberculosis isolates from thailand.the objectives of this study were to characterise mutations in rrs, rpsl and gidb genes in mycobacterium tuberculosis isolates from thailand and to examine possible associations between mutations and strain genotypes. in total, 110 streptomycin (str)-resistant m. tuberculosis isolates and 51 str-susceptible isolates obtained from a sample collection in thailand during 1999-2011 were sequenced for mutation analysis in rrs, rpsl and gidb. genotypes of the isolates were identified using spoligotypi ...201627436385
haarlem 3 is the predominant genotype family in multidrug-resistant and extensively drug-resistant mycobacterium tuberculosis in the capital of iran: a 5-year survey.the objective of this study was to further understand the genetic diversity of multidrug-resistant (mdr) and extensively drug-resistant (xdr) mycobacterium tuberculosis isolates prevalent in tehran, the capital city of iran. from january 2010 to march 2015, a total of 723 m. tuberculosis strains were isolated from patients with pulmonary tuberculosis (tb). a total of 23 mdr, pre-xdr and xdr m. tuberculosis isolates were genotyped by spoligotyping and 24-loci mycobacterial interspersed repetitive ...201627436458
reduction of minimum inhibitory concentrations in drug-resistant mycobacterium tuberculosis isolates in the presence of efflux pump inhibitors. 201627436475
katg ser315 and rpob 81-bp hotspot region substitutions: reliability for detection of drug-resistant strains of mycobacterium tuberculosis. 201627436477
dehydrozingerone inspired styryl hydrazine thiazole hybrids as promising class of antimycobacterial agents.series of styryl hydrazine thiazole hybrids inspired from dehydrozingerone (dzg) scaffold were designed and synthesized by molecular hybridization approach. in vitro antimycobacterial activity of synthesized compounds was evaluated against mycobacterium tuberculosis h37rv strain. among the series, compound 6o exhibited significant activity (mic = 1.5 μm; ic50 = 0.48 μm) along with bactericidal (mbc = 12 μm) and intracellular antimycobacterial activities (ic50 = <0.098 μm). furthermore, 6o displa ...201627437078
mesenteric panniculitis in a thirteen-year-old korean boy treated with prednisolone: a case report.pediatric mesenteric panniculitis is an extremely rare disease of unknown etiology characterized by chronic inflammation, fat necrosis, and fibrosis in the mesenteric adipose tissue. a previously healthy 13-year-old boy was admitted because of right upper abdominal pain. an abdominal computed tomography scan revealed increased attenuation and enhancement in the left upper abdominal omental fat and anterior peritoneal wall thickening. a laparoscopic biopsy showed mesenteric panniculitis with chro ...201627437192
comparative study of genexpert with zn stain and culture in samples of suspected pulmonary tuberculosis.tuberculosis remains one of the deadliest communicable diseases. there are number of tests available for the diagnosis of tuberculosis but conventional microscopy has low sensitivity and culture although gold standard, but takes longer time for positivity. on the other side, nucleic acid amplification techniques due to its rapidity and sensitivity not only help in early diagnosis and management of tuberculosis especially in patients with high clinical suspicion like immunocompromised patients, h ...201627437212
bioactive potential of actinomycetes from less explored ecosystems against mycobacterium tuberculosis and other nonmycobacterial pathogens.bioactive potential of actinomycetes isolated from certain less explored indian ecosystems against mycobacterium tuberculosis and other nonmycobacterial pathogens was investigated. actinomycetes were isolated from the soil samples collected from desert, coffee plantation, rubber forest, and hill area and their cultural and micromorphological characteristics were studied. crude extracts were prepared by agar surface fermentation and tested against m. tuberculosis isolates by luciferase reporter p ...201427437460
clinical profile of patients with tubercular subretinal abscess in a tertiary eye care center in southern india.to retrospectively analyze clinical features, laboratory investigations, treatment and visual outcomes in patients with tubercular subretinal abscess.201627437721
molecular identification of mycobacterium bovis from cattle and human host in mali: expanded genetic diversity.bovine tuberculosis (btb) is a contagious, debilitating human and animal disease caused by mycobacterium bovis, a member of the mycobacterium tuberculosis complex. the study objective were to estimate the frequency of btb, examine genetic diversity of the m. bovis population in cattle from five regions in mali and to determine whether m. bovis is involved in active tuberculosis (tb) in humans. samples from suspected lesions on cattle at the slaughterhouses were collected. mycobacterial smear, cu ...201627439708
first evaluation of genotype mtbdrplus 2.0 performed directly on respiratory specimens in central america.the turnaround times for conventional methods used to detect mycobacterium tuberculosis in sputum samples and to obtain drug susceptibility information are long in many developing countries, including panama, leading to delays in appropriate treatment initiation and continued transmission in the community. we evaluated the performance of a molecular line probe assay, the genotype mtbdrplus version 2.0 assay, in detecting m. tuberculosis complex directly in respiratory specimens from smear-positi ...201627440816
mycobacterium tuberculosis induces expansion of foxp3 positive cd4 t-cells with a regulatory profile in tuberculin non-sensitized healthy subjects: implications for effective immunization against tb.infection by mtb or exposure to mtb constituents is associated with intense microbial stimulation of the immune system, through both antigenic and tlr components, and induction of a milieu that is rich in pro-inflammatory/anti-inflammatory cytokines. here, we addressed the basis of induced regulatory t-cell (it-reg) expansion in response to mtb stimulation, in the absence of prior t cell antigen responsiveness.201627441095
rapid screening of mdr-tb in cases of extra pulmonary tuberculosis using geno type mtbdrplus.drug resistance in tuberculosis is a major public health challenge in developing countries. the limited data available on drug resistance in extra pulmonary tuberculosis stimulated us to design our study on anti-tuberculosis drug resistance pattern in cases of extra pulmonary tuberculosis in a tertiary referral hospital of north india. we performed geno type mtbdrplus assay in comparison with conventional drug susceptibility testing by proportion method to study the mutation patterns in rpob, ka ...201627441660
loc-sers: a promising closed system for the identification of mycobacteria.a closed droplet based lab-on-a-chip (loc) device has been developed for the differentiation of six species of mycobacteria, i.e., both mycobacterium tuberculosis complex (mtc) and nontuberculous mycobacteria (ntm), using surface-enhanced raman spectroscopy (sers). the combination of a fast and simple bead-beating module for the disruption of the bacterial cell with the loc-sers device enables the application of an easy and reliable system for bacteria discrimination. without extraction or furth ...201627441738
mycobacteria and autophagy: many questions and few answers.tuberculosis (tb) is an ancient disease caused by mycobacterium tuberculosis (mtb). tb is one of the world's deadliest diseases, with one-third of infected individuals falling ill each year especially in many developing countries. upon invading host cells, such as macrophages, mtb can replicate in infected cells by arresting phagosome maturation and then potentially escaping into the cytosol. host cells have a mechanism to control intracellular mtb by inducing autophagy, which is an elaborate ce ...201727443861
[cost-effectiveness study of the microbiological diagnosis of tuberculosis using genexpert mtb/rif(®)].to perform a cost-effectiveness analysis of a molecular biology technique for the diagnosis of tuberculosis compared to the classical diagnostic alternative.201627445177
3d correlative light and electron microscopy of cultured cells using serial blockface scanning electron microscopy.the processes of life take place in multiple dimensions, but imaging these processes in even three dimensions is challenging. here, we describe a workflow for 3d correlative light and electron microscopy (clem) of cell monolayers using fluorescence microscopy to identify and follow biological events, combined with serial blockface scanning electron microscopy to analyse the underlying ultrastructure. the workflow encompasses all steps from cell culture to sample processing, imaging strategy, and ...201727445312
espr-dependent esat-6 protein secretion of mycobacterium tuberculosis requires the presence of virulence regulator phop.attenuation of mycobacterium bovis bcg strain is related to the loss of the rd1-encoded esx-1 secretion system. the esx-1 system secretes virulence factor esat-6 that plays a critical role in modulation of the host immune system, which is essential for establishment of a productive infection. previous studies suggest that among the reasons for attenuation of mycobacterium tuberculosis h37ra is a mutation in the phop gene that interferes with the esx-1 secretion system and inhibits secretion of e ...201627445330
toxin-antitoxin systems in clinical pathogens.toxin-antitoxin (ta) systems are prevalent in bacteria and archaea. although not essential for normal cell growth, ta systems are implicated in multiple cellular functions associated with survival under stress conditions. clinical strains of bacteria are currently causing major human health problems as a result of their multidrug resistance, persistence and strong pathogenicity. here, we present a review of the ta systems described to date and their biological role in human pathogens belonging t ...201627447671
antimycobacterial activity of pyrazinoate prodrugs in replicating and non-replicating mycobacterium tuberculosis.tuberculosis (tb) is an important infectious disease caused by mycobacterium tuberculosis (mtb) and responsible for thousands of deaths every year. although there are antimycobacterial drugs available in therapeutics, just few new chemical entities have reached clinical trials, and in fact, since introduction of rifampin only two important drugs had reached the market. pyrazinoic acid (poa), the active agent of pyrazinamide, has been explored through prodrug approach to achieve novel molecules w ...201627449999
gender biased immune-biomarkers in active tuberculosis and correlation of their profiles to efficacy of therapy.active pulmonary tb is an inflammatory disease and is increasingly viewed as an imbalance of immune responses to mycobacterium tuberculosis (m. tb.) infection. in addition, this immune imbalance may be gender biased (males have a higher prevalence of tb) but reasons for such bias are uncertain. we hypothesized that studies on profiles of immune-biomarkers will not only provide insight into molecular basis of gender bias but may also help identify biomarkers to monitor efficacy of tb therapy. we ...201627450000
influence of phthiocerol dimycocerosate on cd4(+) t cell priming and persistence during mycobacterium tuberculosis infection.the characterisation of mycobacterial factors that influence or modulate the host immune response may aid the development of more efficacious tb vaccines. we have previously reported that mycobacterium tuberculosis deficient in export of phthiocerol dimycocerosates (dim) (mt103(δdrrc)) is more attenuated than wild type m. tuberculosis and provides sustained protective immunity compared to the existing bcg vaccine. here we sought to define the correlates of immunity associated with dim deficiency ...201627450001
a quantitative adaptation of the wayne test for pyrazinamide resistance.pyrazinamide (pza) is the most important drug against the latent stage of tuberculosis (tb) and is used in both first and second line treatment regimens. the continued increase in multi-drug resistant tb and the prevalence of pza resistance makes the development of alternative assays for prompt identification of pza resistance all the more important.201627450003
digital pcr assay detection of circulating mycobacterium tuberculosis dna in pulmonary tuberculosis patient plasma.nucleic acid amplification tests are a major diagnostic tool for pulmonary tuberculosis (ptb). recently, digital pcr (dpcr) assay has improved sensitivity for the detection of small copy numbers of target molecules. the aim of this study is to explore the utility of dpcr for detecting mycobacterium tuberculosis (mtb) dna in ptb patient plasma. total dna was purified from plasma samples of newly diagnosed sputum smear-positive ptb patients. copy numbers of mtb-specific genes in the samples were q ...201627450004
mycobacterium tuberculosis strain 18b, a useful non-virulent streptomycin dependent mutant to study latent tuberculosis as well as for in vivo and in vitro testing of anti-tuberculosis drugs. 201627450005
remap: operon map of m. tuberculosis based on rna sequence data.a map of the transcriptional organization of genes of an organism is a basic tool that is necessary to understand and facilitate a more accurate genetic manipulation of the organism. operon maps are largely generated by computational prediction programs that rely on gene conservation and genome architecture and may not be physiologically relevant. with the widespread use of rna sequencing (rnaseq), the prediction of operons based on actual transcriptome sequencing rather than computational genom ...201627450008
evaluation of il-2, il-10, il-17 and ip-10 as potent discriminative markers for active tuberculosis among pulmonary tuberculosis suspects.although interferon gamma release assays (igras) are useful for specifically detecting mycobacterium tuberculosis, they are limited by their inability to differentiate between active tuberculosis (active tb), latent tuberculosis infection (ltbi), and patients with prior tb infection. the purpose of this study was to rapidly and accurately identify active tb patients among patients with suspected respiratory tb by combining interferon-gamma (ifn-γ) with additional cytokines.201627450011
prevalence of pyrazinamide resistance across the spectrum of drug resistant phenotypes of mycobacterium tuberculosis.pyrazinamide resistance is largely unknown in the spectrum of drug resistant phenotypes. we summarize data on pza resistance in clinical isolates from south africa. pza dst should be performed when considering its inclusion in treatment of patients with rifampicin-resistant tb or mdr-tb.201627450014
defining dormancy in mycobacterial disease.tuberculosis remains a threat to global health and recent attempts to shorten therapy have not succeeded mainly due to cases of clinical relapse. this has focussed attention on the importance of "dormancy" in tuberculosis. there are a number of different definitions of the term and a similar multiplicity of different in vitro and in vivo models. the danger with this is the implicit assumption of equivalence between the terms and models, which will make even more difficult to unravel this complex ...201627450015
detection and characterization of drug-resistant conferring genes in mycobacterium tuberculosis complex strains: a prospective study in two distant regions of ghana.we spoligotyped and screened 1490 clinical mycobacterium tuberculosis complex strains from northern and greater accra regions of ghana against inh and rif using the microplate alamar blue phenotypic assay. specific drug resistance associated genetic elements of drug resistant strains were analyzed for mutations. a total of 111 (7.5%), 10 (0.7%) and 40 (2.6%) were mono-resistant to inh, rif, and mdr, respectively. we found the ghana spoligotype to be associated with drug resistance (inh: 22.1%; p ...201627450017
diguanylate cyclase activity of the mycobacterium leprae t cell antigen ml1419c.the second messenger, bis-(3',5')-cyclic dimeric guanosine monophosphate (cyclic di-gmp), is involved in the control of multiple bacterial phenotypes, including those that impact host-pathogen interactions. bioinformatics analyses predicted that mycobacterium leprae, an obligate intracellular bacterium and the causative agent of leprosy, encodes three active diguanylate cyclases. in contrast, the related pathogen mycobacterium tuberculosis encodes only a single diguanylate cyclase. one of the m. ...201627450520
an outbreak of multidrug-resistant tuberculosis among a family.tuberculosis is a major public health problem and it may be complicated by multidrug-resistant tuberculosis (mdr-tb). wide transmission among immunocompetent contacts of the index case is possible. if you detect tuberculosis in two contacts of the index case, it is called an outbreak. the aim of our paper is to evaluate the characteristics of a mdr-tb outbreak affecting 7 people in a family treated during 2012-2014 in istanbul yedikule training and research hospital for chest disease and thoraci ...201627451825
development of potent chemical antituberculosis agents targeting mycobacterium tuberculosis acetohydroxyacid synthase.mycobacterium tuberculosis acetohydroxyacid synthase (mtb-ahas) has been suggested as a crucial target for antibacterial agents. high-throughput screening of a chemical library was performed to identify potent new inhibitors of mtb-ahas. among the 6800 tested compounds, 15 were identified as potent inhibitors, exhibiting >80-90% inhibition of in vitro mtb-ahas activity at a fixed concentration of 20 µm. five compounds belonging to the triazolopyrimidine structural class showed greater inhibition ...201627451857
microfold cells actively translocate mycobacterium tuberculosis to initiate infection.the prevailing paradigm is that tuberculosis infection is initiated when patrolling alveolar macrophages and dendritic cells within the terminal alveolus ingest inhaled mycobacterium tuberculosis (mtb). however, definitive data for this model are lacking. among the epithelial cells of the upper airway, a specialized epithelial cell known as a microfold cell (m cell) overlies various components of mucosa-associated lymphatic tissue. here, using multiple mouse models, we show that mtb invades via ...201627452467
comparison of cortisol and thyroid hormones between tuberculosis-suspect and healthy elephants of nepal.we compared cortisol and thyroid hormone (t3 and t4) concentrations between tuberculosis (tb)-suspected (n=10) and healthy (n=10) elephants of nepal. whole blood was collected from captive elephants throughout nepal, and tb testing was performed using the elephanttb stat-pak(®) and dpp vettb(®) serological assays that detect antibodies against mycobacterium tuberculosis and m. bovis in elephant serum. cortisol, t3 and t4 were quantified by competitive enzyme immunoassays, and the results showed ...201627452878
[analysis on drug resistance of mycobacterium tuberculosis and influencing factors in six provinces of china].to analyze the drug-resistance of clinical mycobacterium tuberculosis strains isolated from the tuberculosis(tb)patients in six provinces in china and related risk factors, and provide evidences for the effective prevention and treatment of drug resistant tb.201627453102
significance of immune response to mycobacterium tuberculosis culture filtrate protein antigens in cerebrospinal fluid of tuberculous meningitis patients: a search for diagnostic marker.mycobacterium tuberculosis (h37ra) culture filtrate proteins (cfp) are explored as a diagnostic marker for tuberculous meningitis (tbm). cerebrospinal fluid (csf) samples from patients were categorized as confirmed (n = 47), suspected (n = 20), and non-tbm (n = 25) cases. immune response by western blot revealed tbm csf samples are having heterogeneous response to cfp. cfp elisa was 92% sensitive and 38.30% specific. ods of confirmed tbm and non-tbm cases were significantly different (p < 0.0001 ...201727454259
global efforts in the development of vaccines for tuberculosis: requirements for improved vaccines against mycobacterium tuberculosis.currently, more than 9.0 million people develop acute pulmonary tuberculosis (tb) each year and about 1.5 million people worldwide die from this infection. thus, developing vaccines to prevent active tb disease remains a priority. this article discusses recent progress in the development of new vaccines against tb and focusses on the main requirements for development of improved vaccines against mycobacterium tuberculosis (m. tb). over the last two decades, significant progress has been made in ...201627454335
induction of transcription factors, mirnas and cytokines involved in t lymphocyte differentiation in bcg-vaccinated subjects.the bcg vaccine induces a th1 phenotype, which is essential for protection against mycobacterium tuberculosis. however, the effects of bcg vaccination over time on the t helper subpopulation and the micrornas involved in adulthood have not been studied. in the present study, we explored the involvement of micrornas, transcription factors and multifunctional cytokines in bcg vaccination by examining their levels both before and after vaccination of healthy adults. peripheral blood mononuclear cel ...201627454344
[the screening of diagnostic potential of native protein fractions of mycobacterium tuberculosis using technique of immune blotting].the technique of immune blotting was used to analyze surface proteins obtained from cells m tuberculosis exposed to partialmode of delipidization. at that, there were applied serums of patients with tuberculosis of lungs; hiv agents and patients with concomitant infection tuberculosis-aids and also hiv-negative patients without clinical signs of disease of lungs and with chronic diseases of lungs of other etiology the fractions oflow-molecular antigens with molecular mass 6.5-30 kilodaltons beca ...201627455562
tumor necrosis factor and its receptors are crucial to control mycobacterium bovis bacillus calmette-guerin pleural infection in a murine model.tumor necrosis factor (tnf) is crucial to control mycobacterium tuberculosis infection, which remains a leading cause of morbidity and mortality worldwide. tnf blockade compromises host immunity and may cause reactivation of latent infection, resulting in overt pulmonary, pleural, and extrapulmonary tuberculosis. herein, we investigate the roles of tnf and tnf receptors in the control of mycobacterium bovis bacillus calmette-guerin (bcg) pleural infection in a murine model. as controls, wild-typ ...201627456129
convergence of a diabetes mellitus, protein energy malnutrition, and tb epidemic: the neglected elderly population.on a global scale, nearly two billion persons are infected with mycobacterium tuberculosis. from this vast reservoir of latent tuberculosis (tb) infection, a substantial number will develop active tb during their lifetime, with some being able to transmit tb or multi-drug- resistant (mdr) tb to others. there is clinical evidence pointing to a higher prevalence of infectious diseases including tb among individuals with diabetes mellitus (dm). furthermore, ageing and diabetes mellitus may further ...201627456231
controlled fire use in early humans might have triggered the evolutionary emergence of tuberculosis.tuberculosis (tb) is caused by the mycobacterium tuberculosis complex (mtbc), a wildly successful group of organisms and the leading cause of death resulting from a single bacterial pathogen worldwide. it is generally accepted that mtbc established itself in human populations in africa and that animal-infecting strains diverged from human strains. however, the precise causal factors of tb emergence remain unknown. here, we propose that the advent of controlled fire use in early humans created th ...201627457933
multifocal tuberculous osteomyelitis in a 3-year-old child.multifocal skeletal tuberculosis accounts for less than 1% of all tuberculous lesions in children. a 3-year-old unimmunised child presented with painless symmetrical swelling of both arms, pallor and hepatosplenomegaly. a skeletal survey demonstrated lytic lesions of the metadiaphysis of both humeri, a rib and the sacrum. the possibility of langerhans cell histiocytosis or multifocal fibrous dysplasia was considered; however, acid-fast bacilli were detected in the biopsy specimen and real-time p ...201727458046
amikacin optimal exposure targets in the hollow-fiber system model of tuberculosis.aminoglycosides such as amikacin are currently used for the treatment of multidrug-resistant tuberculosis (mdr-tb). however, formal pharmacokinetic/pharmacodynamic (pk/pd) studies to identify amikacin exposures and dosing schedules that optimize mycobacterium tuberculosis killing have not been performed. it is believed that aminoglycosides do not work well under acidic conditions, which, if true, would mean poor sterilizing activity against semidormant bacilli at low ph. we performed time-kill s ...201627458215
correction for molina-moya et al., evaluation of genoflow dr-mtb array test for detection of rifampin and isoniazid resistance in mycobacterium tuberculosis. 201627458275
a recombinant trivalent fusion protein f1-lcrv-hsp70(ii) augments humoral and cellular immune responses and imparts full protection against yersinia pestis.plague is one of the most dangerous infections in humans caused by yersinia pestis, a gram-negative bacterium. despite of an overwhelming research success, no ideal vaccine against plague is available yet. it is well established that f1/lcrv based vaccine requires a strong cellular immune response for complete protection against plague. in our earlier study, we demonstrated that hsp70(ii) of mycobacterium tuberculosis modulates the humoral and cellular immunity of f1/lcrv vaccine candidates indi ...201627458447
genotypic characterization of drug resistant mycobacterium tuberculosis in quebec, 2002-2012.the increasing emergence of drug-resistant tuberculosis presents a threat to the effective control of tuberculosis (tb). rapid detection of drug-resistance is more important than ever to address this scourge. the purpose of this study was to genotypically characterize the first-line antitubercular drug-resistant isolates collected over 11 years in quebec.201627459848
inflammasome activation and function during infection with mycobacterium tuberculosis.tuberculosis (tb) is caused by infection with mycobacterium tuberculosis (mtb) and represents one of the most relevant bacterial diseases worldwide. recent advances have yielded new insights into the molecular basis of the immune response required for restriction of the pathogen and also highlighted determinants of immunopathology in tb. several innate immune mediators including soluble proteins as well as lipid molecules participate in both processes, and their mechanisms of action during tb ha ...201627460810
granulomatous responses in hiv and mycobacterium tuberculosis coinfection. 201627462091
relationship between hiv coinfection, interleukin 10 production, and mycobacterium tuberculosis in human lymph node granulomas.human immunodeficiency virus type 1 (hiv)-infected persons are more susceptible to tuberculosis than hiv-uninfected persons. low peripheral cd4(+) t-cell count is not the sole cause of higher susceptibility, because hiv-infected persons with a high peripheral cd4(+) t-cell count and those prescribed successful antiretroviral therapy (art) remain more prone to active tuberculosis than hiv-uninfected persons. we hypothesized that the increase in susceptibility is caused by the ability of hiv to ma ...201627462092
mycobacterium tuberculosis lineages and anti-tuberculosis drug resistance in reference hospitals across viet nam.mycobacterium tuberculosis, the tuberculosis (tb) pathogen, despite a low level of genetic diversity, has revealed a high variety of biological and epidemiological characteristics linked to their lineages, such as transmissibility, fitness and propensity to acquire drug resistance. this has important implications for the epidemiology of tb. we conducted this first countrywide cross-sectional study to identify the prevalent m. tuberculosis lineages and to assess their epidemiological associations ...201627464737
detection of mycobacterium tuberculosis complex in paraffin-embedded tissues by the new automated abbott realtime mtb assay.the abbott realtime mtb assay, launched in june 2014, has been shown to have a competitive performance in the detection of the mycobacterium tuberculosis (mtb) complex in respiratory specimens. the present study was conducted to investigate the usefulness of the abbott mtb realtime assay in the detection of mtb in formalin-fixed paraffin-embedded (ffpe) tissues.201627466302
cd4+ t cells recognizing pe/ppe antigens directly or via cross reactivity are protective against pulmonary mycobacterium tuberculosis infection.mycobacterium tuberculosis (mtb), possesses at least three type vii secretion systems, esx-1, -3 and -5 that are actively involved in pathogenesis and host-pathogen interaction. we recently showed that an attenuated mtb vaccine candidate (mtb δppe25-pe19), which lacks the characteristic esx-5-associated pe/ppe genes, but harbors all other components of the esx-5 system, induces cd4+ t-cell immune responses against non-esx-5-associated pe/ppe protein homologs. these t cells strongly cross-recogni ...201627467705
optimization of structure based virtual screening protocols against thymidine monophosphate kinase inhibitors as antitubercular agents.thymidine monophosphate kinase from mycobacterium tuberculosis (tmpkmtub ) is an established drug target against tuberculosis. the enzyme tmpkmtub is responsible for the survival of bacterium mtb and required to synthesize an essential building block of the bacterial dna which is thymidine triphosphate (ttp). there are several potent inhibitors available against the target enzyme but the majority are substrate analogues. recently, three dimensional structures of the enzyme tmpkmtub inhibitor com ...201127468105
deciphering the structural requirements of nucleoside bisubstrate analogues for inhibition of mbta in mycobacterium tuberculosis: a fb-qsar study and combinatorial library generation for identifying potential hits.the re-emergence of tuberculosis infections, which are resistant to conventional drug therapy, has steadily risen in the last decade. inhibitors of aryl acid adenylating enzyme known as mbta, involved in siderophore biosynthesis in mycobacterium tuberculosis, are being explored as potential antitubercular agents. the ability to identify fragments that interact with a biological target is a key step in fragment based drug design (fbdd). to expand the boundaries of quantitative structure activity ...201127468106
identification of novel inhibitors of udp-galactopyranose mutase by structure-based virtual screening.udp-galactopyranose mutase (ugm) is a flavo-enzyme involved in the bacterial cell wall biosynthesis. ugm catalyzes the reversible isomerization of udp-galactopyranose (udp-galp) to udp-galactofuranose (udp-galf). udp-galf is the activated precursor of galactofuranose (galf) residues that are essential components of the cell wall of certain pathogenic bacteria such as klebsiella pneumoniae and mycobacterium tuberculosis. neither ugm nor galf residues are found in humans, making galf biosynthesis ...201127468107
loperamide restricts intracellular growth of mycobacterium tuberculosis in lung macrophages.new approaches for improving tuberculosis (tb) control using adjunct host-directed cellular and repurposed drug therapies are needed. autophagy plays a crucial role in the response to tb, and a variety of autophagy-inducing drugs that are currently available for various medical conditions may serve as an adjunct treatment in pulmonary tb. here, we evaluated the potential of loperamide, carbamazepine, valproic acid, verapamil, and rapamycin to enhance the antimicrobial immune response to mycobact ...201627468130
c-type lectin receptors in tuberculosis: what we know.mycobacterium tuberculosis (mtb), the etiologic agent of tuberculosis (tb), is recognized by a number of pathogen recognition receptors (prrs), either soluble or predominantly expressed on the surface of various cells of innate and adaptive immunity. c-type lectin receptors (ctlrs) are a class of prrs which can recognize a variety of endogenous and exogenous ligands, thereby playing a crucial role in immunity, as well as in maintaining homeostasis. mtb surface ligands, including mannose-capped l ...201627469378
draft genome sequence of the mycobacterium tuberculosis complex pathogen m. mungi, identified in a banded mongoose (mungos mungo) in northern botswana.mycobacterium mungi, a mycobacterium tuberculosis complex pathogen, has emerged in banded mongoose in northern botswana and northwest zimbabwe. the pathogen is transmitted through infected secretions used in olfactory communication behavior (k. a. alexander, c. e. sanderson, m. h. larsen, s. robbe-austerman, m. c. williams, and m. v. palmer, mbio 7(3):e00281-16, 2016, http://dx.doi.org/10.1128/mbio.00281-16). we announce here the draft genome sequence of this emerging pathogen.201627469947
bortezomib inhibits bacterial and fungal β-carbonic anhydrases.inhibition of the β-carbonic anhydrases (cas, ec 4.2.1.1) from pathogenic fungi (cryptococcus neoformans, candida albicans, candida glabrata, malassezia globosa) and bacteria (three isoforms from mycobacterium tuberculosis, rv3273, rv1284 and rv3588), as well from the insect drosophila melanogaster (dmeca) and the plant flaveria bidentis (fbica1) with the boronic acid peptidomimetic proteosome inhibitor bortezomib was investigated. bortezomib was a micromolar inhibitor of all these enzymes, with ...201627469982
evaluation of quantiferon-tb gold plus for detection of mycobacterium tuberculosis infection in japan.performance of interferon-γ (ifn-γ) release assays still needs to be improved. the data on the performance of quantiferon-tb gold plus (qft-plus), a new-generation of qft assay are limited. this study evaluated the diagnostic performance of qft-plus, and compared to that of quantiferon-tb gold in-tube (qft-git). blood samples were collected from 162 bacteriologically confirmed tuberculosis (tb) patients and 212 mycobacterium tuberculosis-uninfected volunteers; these samples were then tested with ...201627470684
surgical aspects of pulmonary tuberculosis: an update.tuberculosis remains a major global medical challenge and concern. in the world's population of over 7.4 billion people, 8.6 million are estimated to be infected with mycobacterium tuberculosis; another 2.2 billion have latent tuberculosis. there is an annual incidence of 16,000 new cases in the usa and 7-8 million new cases worldwide, of which 440,000 are multidrug-resistant or extensively multidrug-resistant, mainly in developing countries or emerging economies. according to the world health o ...201627471312
the active site architecture in peroxiredoxins: a case study on mycobacterium tuberculosis ahpe.peroxiredoxins catalyze the reduction of peroxides, a process of vital importance to survive oxidative stress. a nucleophilic cysteine, also known as the peroxidatic cysteine, is responsible for this catalytic process. we used the mycobacterium tuberculosis alkyl hydroperoxide reductase e (mtahpe) as a model to investigate the effect of the chemical environment on the specificity of the reaction. using an integrative structural (r116a - pdb ; f37h - pdb ), kinetic and computational approach, we ...201627471753
preparation, standardization and in vitro safety testing of mycobacterium nosodes (emtact- polyvalent nosode).most of the nosodes in the homeopathic pharmacopeia have been sourced from obscure pathological material over a century ago; of which no scientific documentation is available.201627473543
[next generation sequencing for the diagnostics and epidemiology of tuberculosis].tuberculosis (tb) has overtaken hiv (human immunodeficiency virus) and malaria as the leading cause of death by an infectious disease worldwide. the reduction in the tb incidence is a modest 2% of cases per year, thus we will need 200 years to eradicate the disease. part of the problem is that tb control tools are decades old and cannot anymore contribute to accelerate eradication of tb. new diagnostics, treatments and vaccines are urgently needed. next generation sequencing has the potential to ...201627474245
repurposing of a drug scaffold: identification of novel sila analogues of rimonabant as potent antitubercular agents.the structural similarity between an mmpl3 inhibitor bm212, and a cannabinoid receptor modulator rimonabant, prompted us to investigate the anti-tubercular activity of rimonabant and its analogues. further optimization, particularly through incorporation of silicon into the scaffold, resulted in new compounds with significant improvement in anti-tubercular activity against mycobacterium tuberculosis (h37rv). the sila analogue 18a was found to be the most potent antimycobacterial compound (mic, 3 ...201627476117
a convenient synthesis and screening of benzosuberone bearing 1,2,3-triazoles against mycobacterium tuberculosis.a series of benzosuberone bearing 1,2,3-triazoles were rationally designed and alkyl/aryl groups appended on 1,2,3-triazole derivatives 5a-o were synthesized using click chemistry and evaluated for their in vitro antimycobacterial activity against mycobacterium tuberculosis h37rv (atcc27294). compounds 5h (mic: 3.125μg/ml) and 5l, 5m, 5o (mic: 6.25μg/ml) exhibited promising hits. this is the first letter on the synthesis and in vitro antimycobacterial activity against mycobacterium tuberculosis ...201627476139
evolution of mycobacterium tuberculosis and implications for vaccine development.tuberculosis (tb) is a growing public health threat, particularly in the face of the global epidemics of multidrug resistance. given the limited efficacy of the current tb vaccine and the recent clinical failure of the most advanced new tb vaccine candidate, novel concepts for vaccine design should be explored. most t cell antigens in the human-adapted mycobacterium tuberculosis complex (mtbc) are evolutionarily conserved and under strong purifying selection, indicating that host immune response ...201627476231
design and development of new class of mycobacterium tuberculosisl-alanine dehydrogenase inhibitors.mycobacterium tuberculosisl-alanine dehydrogenase (mtb l-aladh) is one of the important drug targets for treating latent/persistent tuberculosis. in this study we used crystal structure of the mtb l-aladh bound with cofactor nad(+) as a structural framework for virtual screening of our in-house database to identified new classes of l-aladh inhibitor. we identified azetidine-2,4-dicarboxamide derivative as one of the potent inhibitor with ic50 of 9.22±0.72μm. further lead optimization by synthesi ...201627477207
mycobacterium tuberculosis and prosthetic joint infection. 201627477980
mycobacterium tuberculosis and prosthetic joint infection. 201627477982
phenotypic analysis of peripheral b cell populations during mycobacterium tuberculosis infection and disease.mycobacterium tuberculosis (mtb) remains an unresolved threat resulting in great annual loss of life. the role of b cells during the protective immunity to mtb is still unclear. b cells have been described as effector cells in addition to their role as antibody producing cells during disease. here we aim to identify and characterize the frequency of peripheral b-cell subpopulations during active tuberculosis and over treatment response. analysis were done for both class switched (cs) and non-cla ...201627478412
interferon-gamma improves macrophages function against m. tuberculosis in multidrug-resistant tuberculosis patients.background. mycobacterium tuberculosis (m. tuberculosis) that causes tuberculosis (tb) kills millions of infected people annually especially multidrug-resistant tuberculosis (mdr-tb). on infection, macrophages recognize the mycobacteria by toll-like receptor (tlr) followed by phagocytosis and control of mycobacteria. in addition, macrophages also secrete il-12 to induce ifn-γ production by t, which, in turn, increases the phagocytosis and oxidative burst. individuals with defects in innate or ad ...201627478636
mycobacterial protein tyrosine phosphatases a and b inhibitors augment the bactericidal activity of the standard anti-tuberculosis regimen.novel drugs are required to shorten the duration of treatment for tuberculosis (tb) and to combat the emergence of drug resistance. one approach has been to identify and target mycobacterium tuberculosis (mtb) virulence factors, which promote the establishment of tb infection and pathogenesis. mtb produces a number of virulence factors, including two protein tyrosine phosphatases (ptps), mptpa and mptpb, to evade the antimicrobial functions of host macrophages. to assess the therapeutic potentia ...201627478867
bioautography with tlc-ms/nmr for rapid discovery of anti-tuberculosis lead compounds from natural sources.while natural products constitute an established source of lead compounds, the classical iterative bioassay-guided isolation process is both time- and labor-intensive and prone to failing to identify active minor constituents. (hp)tlc-bioautography-ms/nmr, which combines cutting-edge microbiological, chromatographic, and spectrometric technologies, was developed to accelerate anti-tuberculosis (tb) drug discovery from natural sources by acquiring structural information at a very early stage of t ...201627478868
the α/β hydrolase fold proteins of mycobacterium tuberculosis, with reference to their contribution to virulence.the α/β hydrolase fold superfamily is an ancient and widely diversified group of primarily hydrolytic enzymes. in this review, the adaptations of these proteins to the pathogenic lifestyle of mycobacterium tuberculosis (mtb), the causative agent of tuberculosis, are examined. of the 105 α/β hydrolases identified in mtb, many are associated with lipid metabolism, particularly in the biosynthesis and maintenance of the mtb's unique cell envelope, as well in the large number of extracellular lipase ...201627480283
mycobacterium tuberculosis gene expression at different stages of hypoxia-induced dormancy and upon resuscitation.the physiology of dormant mycobacterium tuberculosis was studied in detail by examining the gene expression of 51 genes using quantitative reverse-transcription polymerase chain reaction. a forty-day period of dormancy in the wayne culture model depicted four major transcription patterns. some sigma factors and many metabolic genes were constant, whereas genes belonging to the dormancy regulon were activated on day 9. in particular, alpha-crystallin mrna showed more than a 1,000-fold increase co ...201627480637
glutamate racemase is the primary target of β-chloro-d-alanine in mycobacterium tuberculosis.the increasing global prevalence of drug resistance among many leading human pathogens necessitates both the development of antibiotics with novel mechanisms of action and a better understanding of the physiological activities of preexisting clinically effective drugs. inhibition of peptidoglycan (pg) biosynthesis and cross-linking has traditionally enjoyed immense success as an antibiotic target in multiple bacterial pathogens, except in mycobacterium tuberculosis, where it has so far been unde ...201627480853
neonatal fc receptor regulation of lung immunoglobulin and cd103+ dendritic cells confers transient susceptibility to tuberculosis.the neonatal fc receptor (fcrn) extends the systemic half-life of igg antibodies by chaperoning bound fc away from lysosomal degradation inside stromal and hematopoietic cells. fcrn also transports igg across mucosal barriers into the lumen, and yet little is known about how fcrn modulates immunity in the lung during homeostasis or infection. we infected wild-type (wt) and fcrn-deficient (fcgrt(-/-)) mice with pseudomonas aeruginosa or mycobacterium tuberculosis to investigate whether recycling ...201627481246
transduction of functionally contrasting signals by two mycobacterial ppe proteins downstream of tlr2 receptors.as pathogen-associated molecular pattern sensors, the tlrs can detect diverse ligands to elicit either proinflammatory or anti-inflammatory responses, but the mechanism that dictates such contrasting immune responses is not well understood. in this work, we demonstrate that proline-proline-glutamic acid (ppe)17 protein of mycobacterium tuberculosis induces tlr1/2 heterodimerization to elicit proinflammatory-type response, whereas ppe18-induced homodimerization of tlr2 triggers anti-inflammatory ...201627481848
interaction of erp protein of mycobacterium tuberculosis with rv2212 enhances intracellular survival of mycobacterium smegmatis.the mycobacterium tuberculosis exported repetitive protein (rverp) is a crucial virulence-associated factor as determined by its role in the survival and multiplication of mycobacteria in cultured macrophages and in vivo although attempts have been made to understand the function of erp protein, its exact role in mycobacterium pathogenesis is still elusive. one way to determine this is by searching for novel interactions of rverp. using a yeast two-hybrid assay, an adenylyl cyclase (ac), rv2212, ...201627481930
identification of mycobacterium tuberculosis adherence-mediating components: a review of key methods to confirm adhesin function.anti-adhesion therapy represents a potentially promising avenue for the treatment and prevention of tuberculosis in a post-antibiotic era. adhesins are surface-exposed microbial structures or molecules that enable pathogenic organisms to adhere to host surfaces, a fundamental step towards host infection. although several mycobacterium tuberculosis adhesins have been identified, it is predicted that numerous additional adherence-mediating components contribute to the virulence and success of this ...201627482337
mouse model of pulmonary cavitary tuberculosis and expression of matrix metalloproteinase-9.cavitation is a key pathological feature of human tuberculosis (tb), and is a well-recognized risk factor for transmission of infection, relapse after treatment and the emergence of drug resistance. despite intense interest in the mechanisms underlying cavitation and its negative impact on treatment outcomes, there has been limited study of this phenomenon, owing in large part to the limitations of existing animal models. although cavitation does not occur in conventional mouse strains after inf ...201627482816
Refresh
firstprev
...501502503504505506507508509510...
nextlast
Displaying items 50601 - 50700 of 53400