Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| perspectives on mycobacterial vacuole-to-cytosol translocation: the importance of cytosolic access. | mycobacterium tuberculosis, the infectious agent of human tuberculosis is a master player in circumventing the defense mechanisms of the host immune system. the host-pathogen interaction in the case of an infection with m. tuberculosis is highly complex, involving dedicated mycobacterial virulence factors as well as the action of the innate and adapted immune systems, which determine the outcome of infection. macrophages play a key role in this process through internalizing the bacterium in a ph ... | 2016 | 27247079 |
| subtle genotypic changes can be observed soon after diagnosis in mycobacterium tuberculosis infection. | clonal variants of mycobacterium tuberculosis (mtb) coexist in specific patients, although the dynamics of their emergence is unknown. we used miru-vntr to detect microevolution leading to variants of mtb in 3 out of 19 patients (15%) soon after diagnosis (61-85days). most harbored snps and for some of them a potential functional role was suggested. microevolution in tuberculosis seems to occur sooner and more often than expected and could affect tracking of transmission. | 2016 | 27247102 |
| the ins and outs of the mycobacterium tuberculosis-containing vacuole. | the past few years have seen publication of reports from several groups documenting the escape of mycobacterium tuberculosis (mtb) from its intracellular vacuole to access the cytosol. the major questions addressed in these publications are the mechanism(s) underlying this process, the frequency of its occurrence and, most importantly, the biological significance of this phenomenon to bacterial survival, growth and virulence. i believe that the first two questions are moving towards resolution, ... | 2016 | 27247149 |
| standardization of natural mycolic acid antigen composition and production for use in biomarker antibody detection to diagnose active tuberculosis. | mycobacterium tuberculosis, the causative agent of tuberculosis, is characterized by the abundance of species specific, antigenic cell wall lipids called mycolic acids. these wax-like molecules all share an identical, amphiphilic mycolic motif, but have different functional groups in a long hydrophobic hydrocarbon mero-chain that divide them into three main classes: alpha-, keto- and methoxy-mycolic acids. whereas alpha-mycolic acids constitutively maintain an abundance of around 50%, the ratio ... | 2016 | 27247168 |
| targeting mycobacterium tuberculosis tumor necrosis factor alpha-downregulating genes for the development of antituberculous vaccines. | tumor necrosis factor alpha (tnf) plays a critical role in the control of mycobacterium tuberculosis, in part by augmenting t cell responses through promoting macrophage phagolysosomal fusion (thereby optimizing cd4(+) t cell immunity by enhancing antigen presentation) and apoptosis (a process that can lead to cross-priming of cd8(+) t cells). m. tuberculosis can evade antituberculosis (anti-tb) immunity by inhibiting host cell tnf production via expression of specific mycobacterial components. ... | 2016 | 27247233 |
| vaccination against tuberculosis with whole-cell mycobacterial vaccines. | live attenuated and killed whole-cell vaccines (wcvs) offer promising vaccination strategies against tuberculosis. a number of wcv candidates, based on recombinant bacillus calmette-guerin (bcg), attenuated mycobacterium tuberculosis, or related mycobacterial species are in various stages of preclinical or clinical development. in this review, we discuss the vaccine candidates and key factors shaping the development pathway for live and killed wcvs and provide an update on progress. | 2016 | 27247343 |
| activation profile of mycobacterium tuberculosis-specific cd4(+) t cells reflects disease activity irrespective of hiv status. | 2016 | 27248590 | |
| mycobacterium tuberculosis thioredoxin reductase is essential for thiol redox homeostasis but plays a minor role in antioxidant defense. | mycobacterium tuberculosis (mtb) must cope with exogenous oxidative stress imposed by the host. unlike other antioxidant enzymes, mtb's thioredoxin reductase trxb2 has been predicted to be essential not only to fight host defenses but also for in vitro growth. however, the specific physiological role of trxb2 and its importance for mtb pathogenesis remain undefined. here we show that genetic inactivation of thioredoxin reductase perturbed several growth-essential processes, including sulfur and ... | 2016 | 27249779 |
| synthesis and evaluation of new 2-aminothiophenes against mycobacterium tuberculosis. | tuberculosis (tb) and its drug resistant forms kills more people than any other infectious disease. this fact emphasizes the need to identify new drugs to treat tb. 2-aminothiophenes (2at) have been reported to inhibit pks13, a validated anti-tb drug target. we synthesized a library of 42 2at compounds. among these, compound 33 showed remarkable potency against mycobacterium tuberculosis (mtb) h37rv (mic = 0.23 μm) and showed an impressive potency (mic = 0.20-0.44 μm) against mtb strains resista ... | 2016 | 27251120 |
| construction and immunogenicity of a new fc-based subunit vaccine candidate against mycobacterium tuberculosis. | as an ancient disease, tuberculosis (tb) is a major global health threat. therefore, there is an urgent need for an effective and safe anti-tb vaccine. in the current study, a delivery system of fc domain of mouse igg2a and early secreted antigenic target protein 6 (esat-6) was evaluated for the selective uptake of antigens by antigen-presenting cells (apcs). thus, it was based on the immunogenicity of a fusion protein. the study was initiated by the transfer of recombinant expression vectors of ... | 2016 | 27251218 |
| macrophage-mediated inflammatory response decreases mycobacterial survival in mouse mscs by augmenting no production. | mycobacterium tuberculosis (mtb) is a hard-to-eradicate intracellular microbe, which escapes host immune attack during latent infection. recent studies reveal that mesenchymal stem cells (mscs) provide a protective niche for mtb to maintain latency. however, the regulation of mycobacterial residency in mscs in the infectious microenvironment remains largely unknown. here, we found that macrophage-mediated inflammatory response during mtb infection facilitated the clearance of bacilli residing in ... | 2016 | 27251437 |
| vaccine-elicited mucosal and systemic antibody responses are associated with reduced simian immunodeficiency viremia in infant rhesus macaques. | despite significant progress in reducing peripartum mother-to-child transmission (mtct) of human immunodeficiency virus (hiv) with antiretroviral therapy (art), continued access to art throughout the breastfeeding period is still a limiting factor, and breast milk exposure to hiv accounts for up to 44% of mtct. as abstinence from breastfeeding is not recommended, alternative means are needed to prevent mtct of hiv. we have previously shown that oral vaccination at birth with live attenuated myco ... | 2016 | 27252535 |
| toll-like receptor 2 mediates in vivo pro- and anti-inflammatory effects of mycobacterium tuberculosis and modulates autoimmune encephalomyelitis. | mycobacteria display pro- and anti-inflammatory effects in human and experimental pathology. we show here that both effects are mediated by toll-like receptor 2 (tlr2), by exploiting a previously characterized tlr2 variant (met82ile). tlr2 82ile promoted self-specific proinflammatory polarization as well as expansion of ag-specific foxp3(+) tregs, while tlr2 82met impairs the expansion of tregs and reduces the production of ifn-γ and il-17 proinflammatory cytokines. preferential dimerization wit ... | 2016 | 27252700 |
| tuberculosis and leprosy infections in the marshallese population of arkansas, usa. | the cross-immunity between tuberculosis and leprosy is unknown. the aim of this pilot study was to evaluate the occurrence of mycobacterium tuberculosis and m. leprae infection in marshallese adult volunteers in springdale, arkansas, u.s.a., a population that experiences high rates of leprosy and tuberculosis. we used immunodiagnostic testing for tuberculosis and leprosy infection and found significant prevalence of latent tuberculosis infection (19.0%), and asymptomatic mycobacterium leprae inf ... | 2016 | 27255065 |
| pulse proteolysis and precipitation for target identification. | in recent years, phenotypic screening has assumed a leading role in drug discovery efforts. however, development of new drugs from bioactive compounds obtained in screening campaigns requires identification of the cellular targets responsible for their biological activities. a new energetics-based method for target identification is presented: pulse proteolysis and precipitation for target identification (peptid). in this method, proteins incubated with or without a ligand and submitted to a bri ... | 2016 | 27255303 |
| active tuberculosis in psoriasis patients treated with tnf antagonists: a french nationwide retrospective study. | there is limited information about active tuberculosis (tb) occurring in psoriasis patients treated with tumor necrosis factor (tnf) antagonists. | 2016 | 27256154 |
| draft genome sequence of a mycobacterium africanum clinical isolate from antioquia, colombia. | mycobacterium africanum is a member of the mycobacterium tuberculosis complex. most commonly found in west african countries, it has scarcely been described in south america. here, we report the first genome sequence of a colombian m. africanum clinical isolate. it is composed of 4,493,502 bp, with 4,069 genes. | 2016 | 27257203 |
| brief report: hiv-1 infection impairs cd16 and cd35 mediated opsonophagocytosis of mycobacterium tuberculosis by human neutrophils. | using a flow cytometric assay, we investigated neutrophil-mycobacterium tuberculosis opsonophagocytosis and the impact of hiv-1-infected serum on this process. the mean (±sd) percentage of neutrophils internalizing bacilli after 30 minutes incubation was significantly reduced by pretreatment with anti-cd16 (18.2% ± 8.1%, p < 0.001) or anti-cd35 antibody (23.2% ± 10.6%, p < 0.05) versus anti-cd4 controls (29.9% ± 8.1%). blocking cd88 or cd11a did not affect internalization. using heat-inactivated ... | 2016 | 27258232 |
| g protein-coupled receptor160 regulates mycobacteria entry into macrophages by activating erk. | mycobacterium tuberculosis (mtb), the causative agent of tuberculosis, invades and replicates within susceptible hosts by disturbing host antimicrobial mechanisms. although g protein-coupled receptors (gpcrs) are involved in most physiological and pathological activities of mammalian cells, the roles of gpcrs in mtb invasion into host cell remain elusive. here, we report that gpr160 expression is elevated at both mrna and protein level in macrophages in response to bcg infection. both the piggyb ... | 2016 | 27259691 |
| a novel resistance mutation in eccc5 of the esx-5 secretion system confers ofloxacin resistance in mycobacterium tuberculosis. | fluoroquinolone resistance in mycobacterium tuberculosis is often conferred by dna gyrase mutations. however, a substantial proportion of fluoroquinolone-resistant m. tuberculosis isolates do not have such mutations. | 2016 | 27261264 |
| delivery of llkkk18 loaded into self-assembling hyaluronic acid nanogel for tuberculosis treatment. | tuberculosis (tb), a disease caused by the human pathogen mycobacterium tuberculosis, recently joined hiv/aids on the top rank of deadliest infectious diseases. low patient compliance due to the expensive, long-lasting and multi-drug standard therapies often results in treatment failure and emergence of multi-drug resistant strains. in this scope, antimicrobial peptides (amps) arise as promising candidates for tb treatment. here we describe the ability of the exogenous amp llkkk18 to efficiently ... | 2016 | 27261333 |
| induced folding under membrane mimetic and acidic conditions implies undiscovered biological roles of prokaryotic ubiquitin-like protein pup. | ubiquitin-like proteins play important roles in diverse biological processes. in mycobacterium tuberculosis, pup (prokaryotic ubiquitin-like protein), a functional homologue of eukaryotic ubiquitin, interacts with the proteasome atpase subunit mpa to recognize and unfold substrates, and then translocate them into the proteasome core for degradation. previous studies revealed that, pup, an intrinsically disordered protein (idp), adopts a helical structure upon binding to the n-terminal coiled-coi ... | 2016 | 27262305 |
| complement factor h interferes with mycobacterium bovis bcg entry into macrophages and modulates the pro-inflammatory cytokine response. | mycobacterium tuberculosis is an accomplished intracellular pathogen, particularly within the macrophage and this is of the utmost importance in the host-pathogen stand-off observed in the granuloma during latent tuberculosis. contact with innate immune molecules is one of the primary interactions that can occur with the pathogen m. tuberculosis once inhaled. complement proteins may play a role in facilitating m. tuberculosis interactions with macrophages. here, we demonstrate that factor h, a c ... | 2016 | 27262511 |
| antimycobacterial activity of medicinal plants used by the mayo people of sonora, mexico. | tuberculosis (tb) is an infectious disease mainly caused by mycobacterium tuberculosis (mtb), which generates 9 million new cases worldwide each year. the mayo ethnicity of southern sonora, mexico is more than 2000 years old, and the mayos possess extensive knowledge of traditional medicine. | 2016 | 27262564 |
| glucose levels affect ll-37 expression in monocyte-derived macrophages altering the mycobacterium tuberculosis intracellular growth control. | diabetes mellitus (dm)-2 patients have an increased susceptibility to develop pulmonary tuberculosis; this is partly due to the impairment of the innate immunity because of their higher glucose concentrations. in the present study, we determined the effect of the glucose concentrations in the ll-37 expression in infected and non-infected macrophages. our results showed that the increasing glucose concentrations correlates with the low cathelicidin expression in non-infected cells, however in myc ... | 2016 | 27263098 |
| [a case of miliary tuberculosis originated from cutaneous infection]. | an 86-year-old woman with severe dementia had been treated with oral prednisolone at 2 mg/day for autoimmune bullous dermatosis for several years. one year ago, she referred to our hospital due to an ulcerative skin lesion over the right tibial tuberosity. the lesion was treated by an iodine-containing ointment, but did not heal. subsequently, a new skin lesion appeared in the right popliteal fossa. one month ago, the patient had increased sputum production that was accompanied by fever, anorexi ... | 2016 | 27263227 |
| 2-aryl-8-aza-3-deazaadenosine analogues of 5'-o-[n-(salicyl)sulfamoyl]adenosine: nucleoside antibiotics that block siderophore biosynthesis in mycobacterium tuberculosis. | a series of 5'-o-[n-(salicyl)sulfamoyl]-2-aryl-8-aza-3-deazaadenosines were designed to block mycobactin biosynthesis in mycobacterium tuberculosis (mtb) through inhibition of the essential adenylating enzyme mbta. the synthesis of the 2-aryl-8-aza-3-deazaadenosine nucleosides featured sequential copper-free palladium-catalyzed sonogashira coupling of a precursor 4-cyano-5-iodo-1,2,3-triazolonucleoside with terminal alkynes and a minakawa-matsuda annulation reaction. these modified nucleosides w ... | 2016 | 27265685 |
| evaluation of pulmonary microsporidiosis in iatrogenically immunosuppressed patients. | microsporidia spp. are ubiquitous and infect a wide variety of intervertebrates and vertebrates, including humans. pulmonary microsporidiosis, characterized by nonspecific symptoms like fever, cough and dyspnea, is often overlooked in the differential diagnosis of pulmonary infections in immunsupressed patients. in this study, we aimed to determine the prevalence of pulmonary microsporidiosis in iatrogenically immunosuppressed patients and to evaluate the patient characteristics. | 2016 | 27266280 |
| synthesis and antituberculosis activity of the first macrocyclic glycoterpenoids comprising glucosamine and diterpenoid isosteviol. | the first macrocyclic glycoterpenoids comprising glucosamine and diterpenoid isosteviol moieties were synthesized and evaluated for inhibition activity against mycobacterium tuberculosis h37rv. | 2016 | 27267064 |
| a novel dendritic cell-targeted lentiviral vector, encoding ag85a-esat6 fusion gene of mycobacterium tuberculosis, could elicit potent cell-mediated immune responses in mice. | tuberculosis (tb) is an infectious disease caused by mycobacterium tuberculosis (mtb), leading to high mortality worldwide. it is well-established that cellular immunity plays a critical role to control mtb infection. dendritic cells (dcs) are potent antigen presenting cells, which play an important role to prime cell-mediated immune responses. in vivo targeting of dcs has been shown to induce both strong cellular immunity and protection against tumor challenges. the aim of the present study was ... | 2016 | 27267270 |
| laterosporulin10: a novel defensin like class iid bacteriocin from brevibacillus sp. strain skdu10 with inhibitory activity against microbial pathogens. | bacteriocins are antimicrobial peptides (amps) produced by bacteria to acquire survival benefits during competitive inter- and intra-species interactions in complex ecosystems. in this study, an amp-producing soil bacterial strain designated skdu10 was isolated and identified as a member of the genus brevibacillus. the amp produced by strain skdu10 identified as a class iid bacteriocin with 57.6 % homology to laterosporulin, a defensin-like class iid bacteriocin. however, substantial differences ... | 2016 | 27267959 |
| negligible risk of inducing resistance in mycobacterium tuberculosis with single-dose rifampicin as post-exposure prophylaxis for leprosy. | post-exposure prophylaxis (pep) for leprosy is administered as one single dose of rifampicin (sdr) to the contacts of newly diagnosed leprosy patients. sdr reduces the risk of developing leprosy among contacts by around 60 % in the first 2-3 years after receiving sdr. in countries where sdr is currently being implemented under routine programme conditions in defined areas, questions were raised by health authorities and professional bodies about the possible risk of inducing rifampicin resistanc ... | 2016 | 27268059 |
| differential diagnosis and treatment of acute cauda equina syndrome in the human immunodeficiency virus positive patient: a case report and review of the literature. | acute cauda equina syndrome is an uncommon but significant neurologic presentation due to a variety of underlying diseases. anatomical compression of nerve roots, usually by a lumbar disk hernia is a common cause in the general population, while inflammatory, neoplastic, and ischemic causes have also been recognized. among human immunodeficiency virus (hiv) infected patients with acquired immunodeficiency syndrome, infectious causes are encountered more frequently, the most prevalent of which ar ... | 2016 | 27268102 |
| evaluation of xpert® mtb/rif and ustar easynat™ tb iad for diagnosis of tuberculous lymphadenitis of children in tanzania: a prospective descriptive study. | fine needle aspiration biopsy has become a standard approach for diagnosis of peripheral tuberculous lymphadenitis. the aim of this study was to compare the performance of xpert mtb/rif and ustar easynat tb iad nucleic acid amplification assays, against acid-fast bacilli microscopy, cytology and mycobacterial culture for the diagnosis of tb lymphadenitis in children from a tb-endemic setting in tanzania. | 2016 | 27268404 |
| new insights into the mycolate-containing compound biosynthesis and transport in mycobacteria. | mycolic acids are extremely-long-chain fatty acids that compose a large family of mycolate-containing compounds, major envelope lipid components and critical pathogenicity factors of mycobacterium tuberculosis. in recent years there have been major advances in understanding their metabolic pathway. unknown enzymes of the fatty acid synthase type ii elongation system and the condensation system that builds the mycolic acid scaffold were identified. missing links with the mycolate-containing compo ... | 2016 | 27268593 |
| physiologically based pharmacokinetic model of rifapentine and 25-desacetyl rifapentine disposition in humans. | rifapentine (rpt) is a rifamycin antimycobacterial and, as part of a combination therapy, is indicated for the treatment of pulmonary tuberculosis (tb) caused by mycobacterium tuberculosis although the results from a number of studies indicate that rifapentine has the potential to shorten treatment duration and enhance completion rates compared to other rifamycin agents utilized in antituberculosis drug regimens (i.e., regimens 1 to 4), its optimal dose and exposure in humans are unknown. to hel ... | 2016 | 27270284 |
| pyrazinamide is active against mycobacterium tuberculosis cultures at neutral ph and low temperature. | for the past decades, an acidic ph has been used to render mycobacterium tuberculosis susceptible to pyrazinamide for in vitro testing. here, we show that at the standard breakpoint concentration and reduced culture temperatures, pyrazinamide (pza) is active against tuberculosis (tb) at neutral ph. this finding should help unravel the mechanism of action of pza and allow drug susceptibility testing (dst) methods to be optimized. | 2016 | 27270287 |
| loop-mediated isothermal amplification assay targeting the mpb70 gene for rapid differential detection of mycobacterium bovis. | loop-mediated isothermal amplification (lamp) is a highly sensitive, rapid, cost-effective nucleic acid amplification method. tuberculosis (tb) is widely popular in the world and it is difficult to cure. the fundamental treatment is to clear the types of tb pathogens such as mycobacterium bovis (m. bovis), mycobacterium tuberculosis (m. tuberculosis). in order to detect and diagnose tb early, we constructed the differential diagnostic method of tb. in this study, we used lamp for detection of m. ... | 2016 | 27270464 |
| correction: locked nucleic acid probe-based real-time pcr assay for the rapid detection of rifampin-resistant mycobacterium tuberculosis. | [this corrects the article doi: 10.1371/journal.pone.0143444.]. | 2017 | 27271433 |
| a rare case of pott's disease (spinal tuberculosis) mimicking metastatic disease in the southern region of denmark. | pott's disease (pd) or spinal tuberculosis is a rare condition which accounts for less than 1% of total tuberculosis (tb) cases. the incidence of pd has recently increased in europe and the united states, mainly due to immigration; however, it is still a rare diagnosis in scandinavian countries, and if overlooked it might lead to significant neurologic complications. | 2016 | 27272065 |
| nonclassical mhc ib-restricted cd8+ t cells recognize mycobacterium tuberculosis-derived protein antigens and contribute to protection against infection. | mhc ib-restricted cd8+ t cells have been implicated in host defense against mycobacterium tuberculosis (mtb) infection. however, the relative contribution of various mhc ib-restricted t cell populations to anti-mycobacterial immunity remains elusive. in this study, we used mice that lack mhc ia (kb-/-db-/-), mhc ia/h2-m3 (kb-/-db-/-m3-/-), or β2m (β2m-/-) to study the role of m3-restricted and other mhc ib-restricted t cells in immunity against mtb. unlike their dominant role in listeria infecti ... | 2016 | 27272249 |
| a retrospective review of the two-step tuberculin skin test in dialysis patients. | reactivation of latent mycobacterium tuberculosis infection (ltbi) is a health concern for patients on dialysis or receiving a kidney transplant, as these patients are often immunosuppressed. the most frequently used test for ltbi screening in this population is the tuberculin skin test (tst). the diagnostic accuracy (sensitivity and specificity) of the tst in a contemporary north american or western european dialysis population is unknown. | 2016 | 27274397 |
| use of genexpert mycobacterium tuberculosis/rifampicin for rapid detection of rifampicin resistant mycobacterium tuberculosis strains of clinically suspected multi-drug resistance tuberculosis cases. | multi-drug resistance (mdr) tb is defined as tuberculosis (tb) disease caused by a strain of mycobacterium tuberculosis (mtb) that was resistant to at least isoniazid and rifampicin (rif). emerging multidrug-resistant tb is one of the major concerns of health policy and rapid detection of m. tuberculosis and detection of rif resistance in infected patients are essential for disease management. the aim of this study was to evaluate patterns of rif resistance in cases of sputum positive pulmonary ... | 2016 | 27275481 |
| indole-2-carboxamide-based mmpl3 inhibitors show exceptional antitubercular activity in an animal model of tuberculosis infection. | our team had previously identified certain indolecarboxamides that represented a new chemical scaffold that showed promising anti-tb activity at both an in vitro and in vivo level. based on mutational analysis using bacteria found resistant to one of these indolecarboxamides, we identified the trehalose monomycolate transporter mmpl3 as the likely target of these compounds. in the present work, we now further elaborate on the sar of these compounds, which has led in turn to the identification of ... | 2016 | 27275668 |
| lack of association between il-10 gene promoter polymorphisms and susceptible to tuberculosis in thai patients. | cytokines play a major role in defense against mycobacterium tuberculosis infection. polymorphisms in the genes encoding various cytokines have been associated with tuberculosis susceptibility. polymorphisms of the regulatoy cytokine gene, the interleukin (il)-10 is associated with the risk of tuberculosis (tb) in different populations. however il-10 gene polymorphism and susceptibility to tb in thai is still unknown. | 2015 | 27276844 |
| high-resolution melting analysis for molecular detection of multidrug resistance tuberculosis in peruvian isolates. | the emergence of multidrug-resistant strains is a major health problem especially for countries with high tb incidence such as peru. in this study, we evaluated high resolution melting (hrm) assay in peruvian isolates for the detection of mutations within rpob, katg genes and promoter region inha to determine isoniazid and rifampicin resistance in mycobacterium tuberculosis (mtb). | 2016 | 27278526 |
| syndecans promote mycobacterial internalization by lung epithelial cells. | pulmonary tuberculosis (tb) is an airborne disease caused by the intracellular bacterial pathogen mycobacterium tuberculosis (mtb). alveolar epithelial cells and macrophages are the first point of contact for mtb in the respiratory tract. however, the mechanisms of mycobacterial attachment to, and internalization by, nonprofessional phagocytes, such as epithelial cells, remain incompletely understood. we identified syndecan 4 (sdc4) as mycobacterial attachment receptor on alveolar epithelial cel ... | 2016 | 27279134 |
| ag85a/esat-6 chimeric dna vaccine induces an adverse response in tuberculosis-infected mice. | the mycobacterium tuberculosis (m. tb) antigens encoded by the 6 kda early secretory antigenic target (esat-6) and antigen 85a (ag85a) genes are known to exert protective effects against tuberculosis in animal models. in addition, these antigens represent vaccine components that were tested in early human clinical trials. in the present study, a chimeric dna vaccine was constructed that contained two copies of the esat‑6 gene inserted into the ag85a gene from m. tb. balb/c mice were treated with ... | 2016 | 27279275 |
| establishing virulence associated polyphosphate kinase 2 as a drug target for mycobacterium tuberculosis. | inorganic polyphosphate (polyp) plays an essential role in microbial stress adaptation, virulence and drug tolerance. the genome of mycobacterium tuberculosis encodes for two polyphosphate kinases (ppk-1, rv2984 and ppk-2, rv3232c) and polyphosphatases (ppx-1, rv0496 and ppx-2, rv1026) for maintenance of intracellular polyp levels. microbial polyphosphate kinases constitute a molecular mechanism, whereby microorganisms utilize polyp as phosphate donor for synthesis of atp. in the present study w ... | 2016 | 27279366 |
| tackling drug-resistant tuberculosis: current trends and approaches. | tuberculosis is very much rampant in our society and accounts for a large number of deaths annually. in spite of consistent efforts being made, the disease has not been curtailed yet. the emergence of mdr and xdr strains in the society along with an increase in the number of hiv cases and that of latent tb, have further aggravated the problem making the disease very much persistent. the current situation clearly manifests the need to discover and develop new potent molecules/approaches that coul ... | 2017 | 27280980 |
| iron acquisition pathways as targets for antitubercular drugs. | tuberculosis nowadays ranks as the second leading cause of death from an infectious disease worldwide. in the last twenty years, this disease has again started to spread mainly for the appearance of multi-drug resistant forms. therefore, new targets are needed to address the growing emergence of bacterial resistance and for antitubercular drug development. efficient iron acquisition is crucial for the pathogenesis of mycobacterium tuberculosis, because it serves as cofactor in many essential bio ... | 2016 | 27281295 |
| human tuberculosis i. epidemiology, diagnosis and pathogenetic mechanisms. | mycobacterium tuberculosis (m. tuberculosis), an almost genetically monomorphic pathogen is a human parasite, transmitted mostly by humans and causes tuberculosis (tb). tb is firmly associated to poverty, although lack of proper nutrition and lowered immune status are contributing factors for disease development. tb remains second only to hiv/aids as the leading cause of mortality worldwide due to a single infectious agent and is responsible for nearly 1.5 million deaths annually. some steps of ... | 2016 | 27281297 |
| predictive modeling targets thymidylate synthase thyx in mycobacterium tuberculosis. | there is an urgent need to identify new treatments for tuberculosis (tb), a major infectious disease caused by mycobacterium tuberculosis (mtb), which results in 1.5 million deaths each year. we have targeted two essential enzymes in this organism that are promising for antibacterial therapy and reported to be inhibited by naphthoquinones. thyx is an essential thymidylate synthase that is mechanistically and structurally unrelated to the human enzyme. dna gyrase is a dna topoisomerase present in ... | 2016 | 27283217 |
| acute tuberculous myopericarditis mimicking acute myocardial infarction: a case report and literature review. | a number of cases of acute myopericarditis mimicking acute myocardial infarction (ami) have previously been reported in the literature. however, to the best of our knowledge, such a case resulting from mycobacterium tuberculosis infection has not previously been described. the present study reports the case of a 21-year-old male patient presenting with acute chest pain, in whom focal st-segment elevation and elevated cardiac enzymes mimicked a diagnosis of ami. however, acute tuberculous myoperi ... | 2016 | 27284323 |
| variation in c - reactive protein response according to host and mycobacterial characteristics in active tuberculosis. | the c - reactive protein (crp) response is often measured in patients with active tuberculosis (tb) yet little is known about its relationship to clinical features in tb, or whether responses differ between ethnic groups or with different mycobacterium tuberculosis (m.tb) strain types. we report the relationship between baseline serum crp prior to treatment and disease characteristics in a metropolitan population with tb resident in a low tb incidence region. | 2016 | 27287260 |
| spirooxindoles as novel 3d-fragment scaffolds: synthesis and screening against cyp121 from m. tuberculosis. | the search for new scaffolds to complement current hts and fragment libraries is an active area of research. the development of novel strategies to synthesise compounds with 3d character in order to expand the diversity of a fragment library was explored. a range of substituted bicyclo[2,2,1]spirooxindoles were synthesised using a diels-alder [4+2] cycloaddition reaction. both diastereoisomers were isolated from the reactions and these 3d fragment scaffolds were screened against the cytochrome p ... | 2016 | 27287372 |
| a survey of tuberculosis infection control practices at the nih/niaid/daids-supported clinical trial sites in low and middle income countries. | health care associated transmission of mycobacterium tuberculosis (tb) is well described. a previous survey of infection control (ic) practices at clinical research sites in low and middle income countries (lmic) funded by the national institute of allergy and infectious diseases (niaid) conducting hiv research identified issues with respiratory ic practices. a guideline for tb ic based on international recommendations was developed and promulgated. this paper reports on adherence to the guideli ... | 2016 | 27287374 |
| functional divergence of hbha from mycobacterium tuberculosis and its evolutionary relationship with tada from rhodococcus opacus. | rhodococcus opacus pd630 and rhodococcus jostii rha1 are oleaginous bacteria able to synthesize and accumulate triacylglycerols (tag) in lipid bodies (lb). highly relevant to the structure of lb is a protein homologous to heparin-binding hemagglutinin (hbha) (called tada in rhodococci), which is a virulence factor found in mycobacterium tuberculosis. hbha is an adhesin involved in binding to non-phagocytic cells and extrapulmonary dissemination. we observed a conserved synteny of three genes enc ... | 2016 | 27287527 |
| mycobacterium tuberculosis bacteremia in adults and children: a systematic review and meta-analysis. | settingp: among human immunodeficiency virus (hiv) infected adults living in tuberculosis (tb) endemic settings, mycobacterium tuberculosis is a common cause of bloodstream infections. although young children have an increased propensity for m. tuberculosis dissemination, m. tuberculosis bacteremia is infrequently described in children. | 2016 | 27287641 |
| diagnostic accuracy of a uniform research case definition for tbm in children: a prospective study. | bacteriological confirmation of tuberculous meningitis (tbm) is problematic, and rarely guides initial clinical management. a uniform tbm case definition has been proposed for research purposes. | 2016 | 27287642 |
| isoniazid therapy for mycobacterium tuberculosis infection in hiv clinics, los angeles, california. | publicly funded human immunodeficiency virus (hiv) clinics in los angeles county, california, usa. | 2016 | 27287651 |
| quantification of colony-forming units for m. tuberculosis complex using gyrb-based real-time pcr assay. | it is inappropriate to select insertion sequence (is) 6110 as the amplification target for the quantitative analysis of mycobacterium tuberculosis complex (mtc). | 2016 | 27287652 |
| identification of mycobacterium tuberculosis enzyme involved in vitamin d and 7-dehydrocholesterol metabolism. | problems arising during treatment of tuberculosis are well known, therefore studies of mycobacterium drug molecular targets are an area of particular importance. members of the cytochrome p450 family (cyp) may belong to potential candidates for drug targets being involved in metabolism of biologically important molecules in the host organism. cyp124 of mycobacterium tuberculosis (mtcyp124) catalyzes ω-hydroxylation of methyl-branched lipids. the data obtained in the present study indicate that t ... | 2016 | 27289046 |
| identification of novel 2-aminothiazole conjugated nitrofuran as antitubercular and antibacterial agents. | the emergence of antibiotic resistant pathogens is an ongoing main problem in the therapy of bacterial infections. in order to develop promising antitubercular and antibacterial lead compounds, we designed and synthesized a new series of derivatives of 2-aminothiazole conjugated nitrofuran with activities against both mycobacterium tuberculosis and staphylococcus aureus. eight compounds 12e, 12k, 12l, 12m, 18a, 18d, 18e, and 18j emerged as promising antitubercular agents. structure-activity rela ... | 2016 | 27289321 |
| reassessment of the positive predictive value and specificity of xpert mtb/rif: a diagnostic accuracy study in the context of community-wide screening for tuberculosis. | community-wide screening for tuberculosis with xpert mtb/rif as a primary screening tool overcomes some of the limitations of conventional screening. however, concerns exist about the low positive predictive value of this test in screening settings. we did a cross-sectional assessment of this diagnostic test to directly estimate the actual positive predictive value of xpert mtb/rif when used in the setting of community-wide screening for tuberculosis, and to draw an inference about the specifici ... | 2016 | 27289387 |
| [determination of in vitro synergy by a checkerboard method when 3 core antimicrobial agents of the retreatment new scheme combined against mdr-mtb and xdr-mtb]. | in order to detect the in vitro synergistic effect of 4 drugs-pasiniazid (pa), moxifloxacin, rifabutin and rifapentini on multidrug-resistant mycobacterium tuberculosis (mdr-mtb) and extensively drug-resistant mycobacterium tuberculosis(xdr-mtb), which were core drugs of"the program of retreatment research of tuberculosis". | 2016 | 27289577 |
| microrna-206 regulates the secretion of inflammatory cytokines and mmp9 expression by targeting timp3 in mycobacterium tuberculosis-infected thp-1 human macrophages. | tuberculosis (tb) is a serious disease that is characterized by mycobacterium tuberculosis (m.tb)-triggered immune system impairment and lung tissue damage shows limited treatment options. micrornas (mirnas) are regulators of gene expression that play critical roles in many human diseases, and can be up- or downregulated by m.tb infection in macrophage. recently, tissue inhibitor of matrix metalloproteinase (timp) 3 has been found to play roles in regulating macrophage inflammation. here, we fou ... | 2016 | 27291149 |
| complement 3 receptor expression in individuals with type 2 diabetes. | according to the world health organization, as of 2014 9% of the world's adult population is affected by diabetes. uncontrolled diabetes is a pro-inflammatory process that increases generation of reactive oxygen species (ros). | 2016 | 27291248 |
| cheminformatics based machine learning approaches for assessing glycolytic pathway antagonists of mycobacterium tuberculosis. | tuberculosis is the second leading cause of death from an infectious disease worldwide after hiv, thus reasoning the expeditions in antituberculosis research. the rising number of cases of infection by resistant forms of m. tuberculosis has given impetus to the development of novel drugs that have different targets and mechanisms of action against the bacterium. | 2016 | 27291589 |
| structural views along the mycobacterium tuberculosis mend reaction pathway illuminate key aspects of thiamin diphosphate-dependent enzyme mechanisms. | menaquinone (mq) is an essential component of the respiratory chains of many pathogenic organisms, including mycobacterium tuberculosis (mtb). the first committed step in mq biosynthesis is catalyzed by 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase (mend), a thiamin diphosphate (thdp)-dependent enzyme. catalysis proceeds through two covalent intermediates as the substrates 2-oxoglutarate and isochorismate are successively added to the cofactor before final cleavage o ... | 2016 | 27291649 |
| mycobacterium tuberculosis contaminant risk on bone marrow aspiration material from iliac bone patients with active tuberculous spondylitis. | there was a concern on mycobacterium tuberculosis spreading to the bone marrow, when it was applied on tuberculous spine infection. this research aimed to study the probability of using autologous bone marrow as a source of mesenchymal stem cell for patients with tuberculous spondylitis. as many as nine patients with tuberculous spondylitis were used as samples. during the procedure, the vertebral lesion material and iliac bone marrow aspirates were obtained for acid fast staining, bacteria cult ... | 2016 | 27294117 |
| mycobacterial l-forms are found in cord blood: a potential vertical transmission of bcg from vaccinated mothers. | our previous studies showed that mycobacterial l-forms persist in the blood of bcg vaccinated people and that bcg vaccine is able to produce, under appropriate conditions, filterable, self-replicating l-bodies with virus-like size. because filterability is one of the characteristics of l-forms, considerable interest has been shown in their capacity to cross the maternal-fetal barrier. the current study demonstrated isolation of mycobacterial l-form cultures from umbilical cord blood of 5 healthy ... | 2016 | 27294392 |
| twenty years of mycobacterial glycans: furanosides and beyond. | the cell surface (or cell wall) of bacteria is coated with carbohydrate (or glycan) structures that play a number of important roles. these include providing structural integrity, serving as a permeability barrier to extracellular compounds (e.g., drugs) and modulating the immune system of the host. of interest to this account is the cell wall structure of mycobacteria. there are a host of different mycobacterial species, some of which cause human disease. the most well-known is mycobacterium tu ... | 2016 | 27294709 |
| cytotoxic labdane diterpenes from hedychium ellipticum buch.-ham. ex sm. | in order to reveal the constituents and their biological activities, we carried out a phytochemical study on hedychium ellipticum buch.-ham. ex sm. (zingiberaceae). ten labdane diterpenoids (1-10) were isolated from the rhizomes of h. ellipticum for the first time. their structures were identified on the basis of spectroscopic analyses including two-dimensional nmr and comparison with literature data. all of these compounds were evaluated for their antimycobacterial activity against mycobacteriu ... | 2016 | 27294893 |
| clinical presentation, diagnosis, and bacterial epidemiology of peritoneal tuberculosis in two university hospitals in france. | diagnosis of peritoneal tuberculosis (ptb) is difficult, even in developed countries, where data are lacking. the aim of the present study was to describe the clinical presentation, diagnosis, and bacterial epidemiology of ptb in france over a 10-year period. | 2016 | 27295189 |
| zoonotic tuberculosis. a comprehensive one health approach. | the objective of this report is to provide information on mycobacterium tuberculosis complex infections in animals and in humans. included is information on the susceptibility of different species as well as information on etiology, epidemiology, pathogenesis, diagnosis, prevention and control of this disease. the term one health has been adopted to describe the unified human medical and veterinary interdisciplinary/multidisciplinary collaborative approach to zoonoses and will be critical for fu ... | 2016 | 27295705 |
| early detection of multidrug resistant (mdr) mycobacterium tuberculosis in a single tube with in-house designed fluorescence resonance energy transfer (fret) probes using real-time pcr. | rapid and correct diagnosis is crucial for the management of multidrug resistance (mdr) in mycobacterium tuberculosis (mtb). the present study aims at rapid diagnosis for identification of multidrug resistance tuberculosis (mdr-tb) using real-time pcr. fret hybridization probes targeting most prominent four selected codons for rpob526 and 531 and for katg314 and 315 genes were designed and evaluated on 143 clinical mtb isolates and paired sputa for rapid detection of mdr-tb. the results of real- ... | 2016 | 27295919 |
| molecular analysis of idiopathic subglottic stenosis for mycobacterium species. | idiopathic subglottic stenosis (isgs) is an unexplained obstruction involving the lower laryngeal and upper tracheal airway. persistent mucosal inflammation is a hallmark of the disease. epithelial microbiota dysbiosis is found in other chronic inflammatory mucosal diseases; however, the relationship between tracheal microbiota composition and isgs is unknown. given the critical role for host defense at mucosal barriers, we analyzed tissue specimens from isgs patients for the presence of microbi ... | 2017 | 27295947 |
| substantial molecular evolution and mutation rates in prolonged latent mycobacterium tuberculosis infection in humans. | the genome of mycobacterium tuberculosis (mtb) of latently infected individuals may hold the key to understanding the processes that lead to reactivation and progression to clinical disease. we report here analysis of pairs of mtb isolates from putative prolonged latent tb cases. we identified two confirmed cases, and used whole genome sequencing to investigate the mutational processes that occur over decades in latent mtb. we found an estimated mutation rate between 0.2 and 0.3 over 33 years, s ... | 2016 | 27296510 |
| mir-381-3p regulates the antigen-presenting capability of dendritic cells and represses antituberculosis cellular immune responses by targeting cd1c. | tuberculosis is still the widest spread infectious disease in the world, and more in-depth studies are needed on the interaction between the pathogen and the host. due to the highest lipid components in mycobacterium tuberculosis, the cd1 family that specifically presents antigenic lipids plays important roles in the antituberculosis immunity, especially cd1c, which functions as the intracellular ag inspector at the full intracellular range. however, downregulation of the cd1c mrna level has bee ... | 2016 | 27296666 |
| a subset of protective γ9δ2 t cells is activated by novel mycobacterial glycolipid components. | γ9δ2 t cells provide a natural bridge between innate and adaptive immunity, rapidly and potently respond to pathogen infection in mucosal tissues, and are prominently induced by both tuberculosis (tb) infection and bacillus calmette guérin (bcg) vaccination. mycobacterium-expanded γ9δ2 t cells represent only a subset of the phosphoantigen {isopentenyl pyrophosphate [ipp] and (e)-4-hydroxy-3-methyl-but-2-enylpyrophosphate [hmbpp]}-responsive γ9δ2 t cells, expressing an oligoclonal set of t cell r ... | 2016 | 27297390 |
| therapeutic potential of the mycobacterium tuberculosis mycolic acid transporter, mmpl3. | in recent years, whole-cell-based screens for novel small molecule inhibitors active against mycobacterium tuberculosis in culture followed by the whole-genome sequencing of spontaneous resistant mutants have identified multiple chemical scaffolds thought to kill the bacterium through the inactivation of the mycolic acid transporter, mmpl3. consistent with the fact that mmpl3 is required for the formation of the mycobacterial outer membrane, we have conclusively shown in this study, using condit ... | 2016 | 27297488 |
| role of gyrb mutations in pre-extensively and extensively drug-resistant tuberculosis in thai clinical isolates. | dna gyrase mutations are a major cause of quinolone resistance in mycobacterium tuberculosis we therefore conducted the first comprehensive study to determine the diversity of gyrase mutations in pre-extensively drug-resistant (pre-xdr) (n = 71) and extensively drug-resistant (xdr) (n = 30) thai clinical tuberculosis (tb) isolates. all pre-xdr-tb and xdr-tb isolates carried at least one mutation within the quinolone resistance-determining region of gyra (g88a [1.1%], a90v [17.4%], s91p [1.1%], o ... | 2016 | 27297489 |
| antimicrobial resistance prediction in patric and rast. | the emergence and spread of antimicrobial resistance (amr) mechanisms in bacterial pathogens, coupled with the dwindling number of effective antibiotics, has created a global health crisis. being able to identify the genetic mechanisms of amr and predict the resistance phenotypes of bacterial pathogens prior to culturing could inform clinical decision-making and improve reaction time. at patric (http://patricbrc.org/), we have been collecting bacterial genomes with amr metadata for several years ... | 2016 | 27297683 |
| molecular analysis of genetic mutations among cross-resistant second-line injectable drugs reveals a new resistant mutation in mycobacterium tuberculosis. | mutations causing mono and cross-resistance among amikacin, kanamycin and capreomycin of second-line injectable drugs (slids) namely are not well understood. we investigated 124 isolates of mycobacterium tuberculosis for mutations within rrs, eis, tlya and efflux pump (rv1258c and rv0194) genes involved in resistance towards slids. the distribution of mutations across these genes were significantly different in strains with mono-resistance or cross-resistance. a new mutation g878a was found in r ... | 2016 | 27298046 |
| human tuberculosis caused by mycobacterium bovis in the united states, 2006-2013. | using genotyping techniques that have differentiated mycobacterium bovis from mycobacterium tuberculosis since 2005, we review the epidemiology of human tuberculosis caused by m. bovis in the united states and validate previous findings nationally. | 2016 | 27298329 |
| young adult hip: reactivation of dormant, previously undiagnosed mycobacterium tuberculosis infection following intra-articular steroid injection. | tuberculosis (tb) still remains a common problem in the uk and, with the increasing number of patients being offered arthroplasties; periprosthetic involvement is not uncommon anymore. however, the diagnosis of tb infected arthroplasties still remains difficult and misdiagnosis is common, therefore delaying treatment. | 2017 | 27299119 |
| application of the multistate tuberculosis pharmacometric model in patients with rifampicin-treated pulmonary tuberculosis. | this is the first clinical implementation of the multistate tuberculosis pharmacometric (mtp) model describing fast-, slow-, and nonmultiplying bacterial states of mycobacterium tuberculosis. colony forming unit data from 19 patients treated with rifampicin were analyzed. a previously developed rifampicin population pharmacokinetic (pk) model was linked to the mtp model previously developed using in vitro data. drug effect was implemented as exposure-response relationships tested at several effe ... | 2016 | 27299939 |
| evaluation of the inhibitory activity of (aza)isoindolinone-type compounds: toward in vitro inha action, mycobacterium tuberculosis growth and mycolic acid biosynthesis. | inhibitors of the mycobacterium tuberculosis enoyl-acp reductase (inha) are considered as potential promising therapeutics for the treatment of tuberculosis. previously, we reported that azaisoindolinone-type compounds displayed, in vitro, inhibitory activity toward inha. herein, we describe chemical modifications of azaisoindolinone scaffold, the synthesis of 15 new compounds and their evaluations toward the in vitro inha activity. based on these results, a structure-inha inhibitory activity re ... | 2016 | 27301022 |
| potential of immunomodulatory agents as adjunct host-directed therapies for multidrug-resistant tuberculosis. | treatment of multidrug-resistant tuberculosis (mdr-tb) is extremely challenging due to the virulence of the etiologic strains of mycobacterium tuberculosis (m. tb), the aberrant host immune responses and the diminishing treatment options with tb drugs. new treatment regimens incorporating therapeutics targeting both m. tb and host factors are urgently needed to improve the clinical management outcomes of mdr-tb. host-directed therapies (hdt) could avert destructive tuberculous lung pathology, fa ... | 2016 | 27301245 |
| intracardiac tuberculomas caused by mycobacterium tuberculosis in a dog. | this paper presents an unusual form of disseminated mycobacterium tuberculosis infection in a dog. the infection lasted at least one year and its main gross lesions were massive cardiac tuberculomas. to the best of our knowledge, this is the first report of heart tuberculomas in a dog. | 2016 | 27301275 |
| synthesis and antitubercular activity of new 1,3,4-oxadiazoles bearing pyridyl and thiazolyl scaffolds. | in search of more potent and safe new antitubercular agents, here new 2-pyridinyl substituted thiazolyl-5-aryl-1,3,4-oxadiazoles (6a-o), have been designed and synthesized using thionicotinamide as a starting, following novel multistep synthetic route. an intermediate, pyridinyl substituted thiazolyl acid hydrazide (4) when condensed with benzoic acids/nicotinic acids (5a-o) in the presence of silica supported pocl3 yielded better to excellent yields of the title compounds. all the synthesized c ... | 2016 | 27301367 |
| a patient presenting with tuberculous encephalopathy and human immunodeficiency virus infection. | in the usa, mycobacterium tuberculosis infection is more likely to be found in foreign-born individuals, and those co-infected with human immunodeficiency virus (hiv) are more likely to have tuberculous meningitis. the literature is lacking in details about the clinical workup of patients presenting with tuberculous meningitis with encephalopathic features who are co-infected with hiv. this report demonstrates a clinical approach to diagnosis and management of tuberculous meningitis. | 2016 | 27302013 |
| autophagy induction targeting mtorc1 enhances mycobacterium tuberculosis replication in hiv co-infected human macrophages. | to survive and replicate in macrophages mycobacterium tuberculosis (mtb) has developed strategies to subvert host defence mechanisms, including autophagy. autophagy induction has the potential to clear mtb, but little is known about its effect during controlled tuberculosis and hiv co-infection. mammalian target of rapamycin complex1 (mtorc1) inhibitors were used to induce autophagy in human macrophages pre-infected with hiv-1bal and infected with a low dose of mtb (co-infected), or single mtb i ... | 2016 | 27302320 |
| fidelity and promiscuity of a mycobacterial glycosyltransferase. | members of the genus mycobacterium cause devastating human diseases, including tuberculosis. mycobacterium tuberculosis can resist some antibiotics because of its durable and impermeable cell envelope. this barrier is assembled from saccharide building blocks not found in mammals, including galactofuranose (galf). within the cell envelope, galf residues are linked together to afford an essential polysaccharide, termed the galactan. the formation of this polymer is catalyzed by the glycosyltransf ... | 2016 | 27302377 |
| identification of quantitative proteomic differences between mycobacterium tuberculosis lineages with altered virulence. | evidence currently suggests that as a species mycobacterium tuberculosis exhibits very little genomic sequence diversity. despite limited genetic variability, members of the m. tuberculosis complex (mtbc) have been shown to exhibit vast discrepancies in phenotypic presentation in terms of virulence, elicited immune response and transmissibility. here, we used qualitative and quantitative mass spectrometry tools to investigate the proteomes of seven clinically-relevant mycobacterial strains-four ... | 2016 | 27303394 |
| testicular tuberculosis without epididymitis simulating neoplasm. | we present the case of a 47-year-old man with testicular tuberculosis without epididymal involvement that simulated neoplasm on sonography. the patient also had evidence of contralateral spermatic cord involvement. the diagnosis was made following transinguinal intrascrotal exploration and excisional biopsy of the left spermatic cord mass and right transinguinal radical orchiectomy. histopathology showed caseating granulomatous inflammation, with positive cultures for mycobacterium tuberculosis ... | 2008 | 27303531 |
| reactivation mycobacterium tuberculosis presenting as empyema necessitans 55 years following thoracoplasty. | we describe the case of a 79-year-old man who presented with an enlarging mass on his chest wall. he had a history of thoracoplasty performed 55 years ago for treatment of pulmonary tuberculosis. the mass was subsequently proven to be the result of empyema neccesitans caused by reactivation tuberculosis. empyema neccesitans is a well described entity in which an empyema spontaneously decompresses by dissecting into the chest wall and extrathoracic soft tissues. this can occur following necrotizi ... | 2008 | 27303540 |
| targeting drug-sensitive and -resistant strains of mycobacterium tuberculosis by inhibition of src family kinases lowers disease burden and pathology. | in view of emerging drug resistance among bacterial pathogens, including mycobacterium tuberculosis, the development of novel therapeutic strategies is increasingly being sought. a recent paradigm in antituberculosis (anti-tb) drug development is to target the host molecules that are crucial for intracellular survival of the pathogen. we previously showed the importance of src tyrosine kinases in mycobacterial pathogenesis. here, we report that inhibition of src significantly reduced survival of ... | 2017 | 27303736 |
| the secb-like chaperone rv1957 from mycobacterium tuberculosis: crystallization and x-ray crystallographic analysis. | protein export is important in all bacteria, and bacteria have evolved specialized export machineries to fulfil this task. in mycobacterium tuberculosis, the causative agent of tuberculosis, the general secretion pathway (sec pathway) is conserved and is essential in performing the export of proteins. the bacterial sec pathway post-translationally exports unfolded proteins out of the cytoplasm, and the core of the sec pathway is composed of a heterotrimeric membrane-embedded channel, secyeg, and ... | 2016 | 27303898 |