Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| clya cytolysin from salmonella: distribution within the genus, regulation of expression by slya, and pore-forming characteristics. | functional homologs of the escherichia coli cytolysin a (clya, hlye, shea) gene have recently been detected in salmonella enterica serovars typhi (s. typhi) and paratyphi a (s. paratyphi a). in this study, analysis of a collection of salmonella strains showed that all s. typhi and s. paratyphi a strains tested harbor an intact copy of the corresponding clya variant, i.e. clya(sty) and clya(spaa), respectively. on the other hand, clya proved to be absent in the s. enterica serovar paratyphi b and ... | 2009 | 18715828 |
| assessment of the stability of cell-surface components of microorganisms by maldi-tof-ms following preservation on lenticule discs. | strains representing the species campylobacter coli, escherichia coli, listeria monocytogenes, pseudomonas aeruginosa, salmonella enterica, and staphylococcus aureus were randomly selected to assess the consistency of cells preserved on lenticule discs to those archived in traditional freeze-dried ampoules. each matched pair was cultured using identical conditions and analysed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (ms) to profile the surface-associated m ... | 2008 | 18717884 |
| evolution of genes on the salmonella virulence plasmid phylogeny revealed from sequencing of the virulence plasmids of s. enterica serotype dublin and comparative analysis. | salmonella enterica serotype dublin harbors an approximately 80-kb virulence plasmid (psdv), which mediates systemic infection in cattle. there are two types of psdv: one is psdvu (pou1113) in strain ou7025 and the other psdvr (pou1115) in ou7409 (sd lane) and many clinical isolates. sequence analysis showed that psdvr was a recombinant plasmid (co-integrate) of psdvu and a plasmid similar to a 35-kb indigenous plasmid (pou1114) of s. dublin. most of the f-transfer region in psdvu was replaced b ... | 2008 | 18718522 |
| inactivation of bacteria and viruses in human urine depending on temperature and dilution rate. | source separation and reuse of human urine can decrease the environmental pollution of recipient waters and reduce the need for artificial mineral fertilisers. however, the reuse of urine introduces another pathogen transmission route that needs to be managed. the inactivation of enteric pathogens and model organisms (salmonella enterica subspecies 1 serovar typhimurium (s. typhimurium), enterococcus faecalis, bacteriophages s. typhimurium 28b, ms2 and phi x 174) by urine storage was studied at ... | 2008 | 18718625 |
| effect of poultry decontaminants concentration on growth kinetics for pathogenic and spoilage bacteria. | various chemical compounds are currently under review for final approval as poultry decontaminants in the european union (eu). concentration is among the factors considered by the eu authorities in the evaluation of these treatments. the aim of this research was to compare the growth parameters for pathogenic and spoilage bacteria in presence of high and low concentrations of poultry decontaminants to assess whether such treatments could involve a potential sanitary risk for consumers. growth cu ... | 2008 | 18721678 |
| distribution of integrons and sgi1 among antibiotic-resistant salmonella enterica isolates of animal origin. | in salmonella enterica, resistance to antibiotics can be caused by the presence of sgi1, transposons or conjugative plasmids. in this study we were interested in the relative contribution of these genetic elements to the antibiotic resistance of s. enterica isolates collected within a single year in the czech republic from animal sources. altogether 123 antibiotic-resistant isolates belonging to 16 different s. enterica serovars were classified into 3 groups according to the presence of sgi1 and ... | 2009 | 18723300 |
| suppressor analysis of the motb(d33e) mutation to probe bacterial flagellar motor dynamics coupled with proton translocation. | mota and motb form the stator of the proton-driven bacterial flagellar motor, which conducts protons and couples proton flow with motor rotation. asp-33 of salmonella enterica serovar typhimurium motb, which is a putative proton-binding site, is critical for torque generation. however, the mechanism of energy coupling remains unknown. here, we carried out genetic and motility analysis of a slowly motile motb(d33e) mutant and its pseudorevertants. we first confirmed that the poor motility of the ... | 2008 | 18723617 |
| antimicrobial resistance in typhoidal and nontyphoidal salmonellae. | increasing occurrence of antimicrobial resistance in both typhoidal and nontyphoidal salmonellae is a major public health problem. recent studies documenting the occurrence and types of resistance, with particular reference to quinolones and extended spectrum cephalosporins, and new approaches to treatment are reviewed. | 2008 | 18725804 |
| ecology of e. coli o157:h7 and salmonella enterica in the primary vegetable production chain. | there is an increased concern that plants might be more important as a carrier for human enteric pathogens like e. coli o157:h7 and salmonella enterica serovars than previously thought. this review summarizes the knowledge available on the ecology of e. coli o157:h7 and salmonella enterica in the primary production chain of leafy green vegetables (in particular lettuce), including manure, manure-amended soil, and crop. based on the available literature, suggestions are made for the control of th ... | 2008 | 18728991 |
| the pathogenesis of salmonella enteritidis in experimentally infected ducks: a quantitative time-course study using taqman polymerase chain reaction. | ducks were subcutaneously infected with a high-virulence strain of salmonella enterica ssp. enterica serovar enteritidis (salmonella enteritidis). the kinetics of the salmonella enteritidis genomic dna loads, the immunohistochemical localization of the bacterial antigens, and the histopathological examination in various tissues were investigated. the results showed that the time course of the appearance of the salmonella enteritidis bacterial antigens and the lesions in various tissues was coinc ... | 2008 | 18753444 |
| determination of pyrophosphorylated forms of lipid a in gram-negative bacteria using a multivaried mass spectrometric approach. | lipid a isolated from several bacteria (escherichia coli, pseudomonas aeruginosa, salmonella enterica, and various strains of yersinia) showed abundant formation of pyrophosphate anions upon ion dissociation. pyrophosphate [h(3)p(2)o(7)](-) and/or [hp(2)o(6)](-) anions were observed as dominant fragments from diphosphorylated lipid a anions regardless of the ionization mode (matrix-assisted laser desorption ionization or electrospray ionization), excitation mode (collisional activation or infrar ... | 2008 | 18753624 |
| salmonella sp group a: a rare cause of bacterascites. a case report. | bacterascites (ba) is a minimally studied and defined entity. its prognosis and clinical course are not well defined, and currently there are no management guidelines. we present a rare cause of ba in which salmonella sp group a was isolated in a 44 year old man with cirrhosis who had diarrhea and fever three days earlier. treatment with intravenous ceftriaxone was effective. | 2008 | 18753997 |
| occurrence and characteristics of the cytolysin a gene in shigella strains and other members of the family enterobacteriaceae. | cytolysin a (clya, hlye, shea) is a hemolytic pore-forming toxin found in escherichia coli and salmonella enterica serovars typhi and paratyphi a. in the present study, analysis of several shigella strains revealed that they harbor only nonfunctional clya gene copies that have been inactivated either by the integration of insertion sequence (is) elements (shigella dysenteriae, shigella boydii, and shigella sonnei strains) or by a frameshift mutation (shigella flexneri). shigella dysenteriae and ... | 2008 | 18754791 |
| direct and quantitative analysis of salmonella enterica serovar typhimurium using real-time pcr from artificially contaminated chicken meat. | for quantitative pcr assay of salmonella enterica serovar typhimurium in food samples, a real-time pcr method was developed, based on dna genome equivalent. specific primers and probe designed based on the stm4497 gene of s. typhimurium lt2 showed the specificity to s. typhimurium. threshold cycle (ct) values of real-time pcr were obtained from a quantitative standard curve with genomic dna of salmonella typhimurium. in addition, the recovery of s. typhimurium inoculated artificially to chicken ... | 2008 | 18756108 |
| molecular phylogeny of the salmonellae: relationships among salmonella species and subspecies determined from four housekeeping genes and evidence of lateral gene transfer events. | the salmonellae are a diverse group of bacteria within the family enterobacteriaceae that includes two species, salmonella enterica and salmonella bongori. in order to characterize the phylogenetic relationships of the species and subspecies of salmonella, we analyzed four housekeeping genes, gapa, phop, mdh and reca, comprising 3,459 bp of nucleotide sequence data for each isolate sequenced. sixty-one isolates representing the most common serotypes of the seven subspecies of salmonella enterica ... | 2008 | 18757540 |
| antimicrobial resistance in salmonella enterica serovar heidelberg isolates from retail meats, including poultry, from 2002 to 2006. | salmonella enterica serovar heidelberg frequently causes food-borne illness in humans. there are few data on the prevalence, antimicrobial susceptibility, and genetic diversity of salmonella serovar heidelberg isolates in retail meats. we compared the prevalences of salmonella serovar heidelberg in a sampling of 20,295 meats, including chicken breast (n = 5,075), ground turkey (n = 5,044), ground beef (n = 5,100), and pork chops (n = 5,076), collected during 2002 to 2006. isolates were analyzed ... | 2008 | 18757574 |
| the salmonella spi-2 effector ssej exhibits eukaryotic activator-dependent phospholipase a and glycerophospholipid : cholesterol acyltransferase activity. | intracellular replication of salmonella enterica serovar typhimurium within membrane-bound compartments, called salmonella-containing vacuoles, depends on the activities of several effector proteins translocated by the salmonella pathogenicity island 2 (spi-2)-encoded type iii secretion system. the spi-2 effector protein ssej shows similarity at the amino acid level to several gdsl lipases with glycerophospholipid : cholesterol acyltransferase (gcat) activity. in this study, we show that catalyt ... | 2008 | 18757801 |
| the lysr-type transcriptional regulator hrg counteracts phagocyte oxidative burst and imparts survival advantage to salmonella enterica serovar typhimurium. | lysr-type transcriptional regulators (lttrs) are one of the key players that help bacteria adapt to different environments. we have designated stm0952, a putative lttr in salmonella enterica serovar typhimurium (s. typhimurium), as hydrogen peroxide resistance gene (hrg). a hrg knockout mutant of s. typhimurium was sensitive to oxidative products of the respiratory burst, specifically to h(2)o(2). the hrg mutant is profoundly attenuated in a murine model of infection and showed decreased intrace ... | 2008 | 18757817 |
| the mechanism of ciprofloxacin resistance in dihydrogen peroxide-induced mutants of salmonella enterica subsp. enterica serovar typhimurium consists mainly in mutations in gyra gene and less in mutations affecting ciprofloxacin uptake. | the effect of h(2)o(2) on the induction of ciprofloxacin (cfl) resistant mutants of salmonella enterica subsp. enterica serovar typhimurium was evaluated and determinants of cfl resistance in the mutants were analyzed. factors associated with cfl resistance in h(2)o(2)-induced mutants included (i) mutations in gyra gene, predominantly (63 %) asp(87)-->asn and less (37 %) ser(83)-->phe substitutions, (ii) mutations in the regulatory genes of marrab or soxrs or in the individual structural genes o ... | 2008 | 18759123 |
| cytolocalization of the phop response regulator in salmonella enterica: modulation by extracellular mg2+ and by the scv environment. | the phop/phoq two-component system plays an essential role regulating numerous virulence phenotypes in salmonella enterica. previous work showed that phoq, the sensor protein, switches between the kinase- and the phosphatase-dominant state in response to environmental mg2+ availability. this switch defines the phop phosphorylation status and, as a result, the transcriptional activity of this regulator. in this work, using the flash labelling technique, we examine phop cytolocalization in respons ... | 2008 | 18761685 |
| nationwide outbreak of salmonella enterica serotype kedougou infection in infants linked to infant formula milk, spain, 2008. | 2008 | 18761885 | |
| first report of a salmonella enterica serovar weltevreden outbreak on reunion island, france, august 2007. | an outbreak of gastroenteritis involving 26 guests of a wedding dinner occurred in august 2007 in réunion island, a french overseas department. salmonella was isolated in 61.5% of cases and the two isolates serotyped were of serovar weltevreden. we believe this to be the first food-borne outbreak due to s. enterica serovar weltevreden described in réunion island. the epidemiological and environmental investigations of this outbreak did not provide enough evidence to identify a single vehicle of ... | 2008 | 18761897 |
| effects of a commercially available vaccine against salmonella enterica serotype newport on milk production, somatic cell count, and shedding of salmonella organisms in female dairy cattle with no clinical signs of salmonellosis. | to determine effects of vaccination with siderophore receptor and porin (srp) proteins derived from salmonella enterica serotype newport on milk production, somatic cell count, and shedding of salmonella organisms in female dairy cattle. | 2008 | 18764698 |
| functional analysis of the salmonella pathogenicity island 2-mediated inhibition of antigen presentation in dendritic cells. | salmonella enterica is a facultative intracellular pathogen that is able to modify host cell functions by means of effector proteins translocated by the type iii secretion system (t3ss) encoded by salmonella pathogenicity island 2 (spi2). the spi2-t3ss is also active in salmonella after uptake by murine bone marrow-derived dendritic cells (bm-dc). we have previously shown that intracellular salmonella interfere with the ability of bm-dc to stimulate antigen-dependent t-cell proliferation in an s ... | 2008 | 18765734 |
| a sopb deletion mutation enhances the immunogenicity and protective efficacy of a heterologous antigen delivered by live attenuated salmonella enterica vaccines. | sopb is a virulence factor of salmonella encoded by spi-5. salmonella sopb deletion mutants are impaired in their ability to cause local inflammatory responses and fluid secretion into the intestinal lumen and also can enhance the immunogenicity of a vectored antigen. in this study, we evaluated the effects on immunogenicity and the efficacy of a sopb deletion mutation on two salmonella enterica serovar typhimurium vaccine strains with different attenuating mutations expressing a highly antigeni ... | 2008 | 18765737 |
| pir-b-deficient mice are susceptible to salmonella infection. | paired ig-like receptors of activating (pir-a) and inhibitory (pir-b) isoforms are expressed by many hematopoietic cells, including b lymphocytes and myeloid cells. to determine the functional roles of pir-a and pir-b in primary bacterial infection, pir-b-deficient (pir-b(-/-)) and wild-type (wt) control mice were injected i.v. with an attenuated strain of salmonella enterica typhimurium (wb335). pir-b(-/-) mice were found to be more susceptible to salmonella infection than wt mice, as evidenced ... | 2008 | 18768880 |
| the in vitro anti-pathogenic activity of immunoglobulin concentrates extracted from ovine blood. | an immunoglobulin-rich fraction has been prepared from ovine blood in our laboratory. we have investigated its antibacterial activity and binding activity to pathogenic whole cell antigens, lipopolysaccharide (lps) and staphylococcal enterotoxin b. ovine immunoglobulin concentrate (oic) comprised about 73 +/- 2% of igg and 11 +/- 1% of igm on a protein basis. it inhibited the growth of all 13 strains of pathogens tested, but the inhibitory activity varied according to bacterial strain. the inhib ... | 2009 | 18769875 |
| rapid allelic exchange in enterohemorrhagic escherichia coli (ehec) and other e. coli using lambda red recombination. | this unit describes an allelic exchange system for enterohemorrhagic e. coli (ehec), and similar pathogenic species of bacteria. the phage lambda red recombination system is expressed from a plasmid, inducing a hyper-recombinogenic state where electroporated pcr-generated substrates recombine with the bacterial chromosome at high efficiency. the technique can be used to substitute a drug marker for the gene of interest, or used to generate a clean in-frame deletion of the target gene. single gen ... | 2006 | 18770591 |
| salmonella enterica serovar enteritidis genes induced during oviduct colonization and egg contamination in laying hens. | salmonella enterica serovar enteritidis is the predominant serovar associated with salmonellosis worldwide, which is in part due to its ability to contaminate the internal contents of the hen's egg. it has been shown that s. enterica serovar enteritidis has an unusual tropism for the avian reproductive tract and an ability to persist in the oviduct and ovary. factors allowing s. enterica serovar enteritidis strains to contaminate eggs could be a specific interaction with the oviduct tissue, lead ... | 2008 | 18776023 |
| comparison of five culture methods for salmonella isolation from swine fecal samples of known infection status. | the current study was conducted to evaluate 5 bacteriologic culture methods (methods 1, 2, 3, 4, and 5) for recovery of salmonella enterica from swine feces, both for sensitivity of detection (ability to recover salmonella from a positive sample) and for specificity (not to inadvertently identify an organism as a salmonella species in a negative sample). fifty-six negative samples and 46 positive samples were processed using each of the 5 methods, which differed primarily in the combinations of ... | 2008 | 18776096 |
| salmonella-containing vacuoles: directing traffic and nesting to grow. | salmonella enterica serovar typhimurium (s. typhimurium) is a gram-negative facultative intracellular pathogen that can infect a broad range of mammalian hosts. following invasion of host cells, the majority of s. typhimurium are known to reside in a membrane-bound compartment known as the salmonella-containing vacuole (scv). s. typhimurium actively remodels this compartment using bacterial virulence proteins, called effectors, to establish a protected niche where it can replicate. s. typhimuriu ... | 2008 | 18778407 |
| the pagn protein of salmonella enterica serovar typhimurium is an adhesin and invasin. | the pagn gene of salmonella enterica serovar typhimurium is a phop-regulated gene that is up-regulated during growth within macrophages and in vivo in murine models of infection. the pagn protein displays similarity to the hek and tia invasins/adhesins of escherichia coli. thus far no function has been ascribed to the pagn protein. | 2008 | 18778463 |
| plasmid-mediated quinolone resistance in nalidixic-acid-susceptible strains of salmonella enterica isolated in scotland. | 2008 | 18779257 | |
| membrane permeability and regulation of drug "influx and efflux" in enterobacterial pathogens. | in enterobacteriaceae, membrane permeability is a key in the level of susceptibility to antibiotics. modification of the bacterial envelope by decreasing the porin production or increasing the expression of efflux pump systems has been reported. these phenomena are frequently associated with other resistance mechanisms such as alteration of antibiotics or modification of the drug targets, in various clinical isolates showing a multi drug resistant phenotype (mdr). in escherichia coli, enterobact ... | 2008 | 18781921 |
| structure of the pduu shell protein from the pdu microcompartment of salmonella. | the pdu microcompartment is a proteinaceous, subcellular structure that serves as an organelle for the metabolism of 1,2-propanediol in salmonella enterica. it encapsulates several related enzymes within a shell composed of a few thousand protein subunits. recent structural studies on the carboxysome, a related microcompartment involved in co(2) fixation, have concluded that the major shell proteins from that microcompartment form hexamers that pack into layers comprising the facets of the shell ... | 2008 | 18786396 |
| salmonella infections in antarctic fauna and island populations of wildlife exposed to human activities in coastal areas of australia. | salmonella infections in antarctic wildlife were first reported in 1970 and in a search for evidence linking isolations with exposure to human activities, a comparison was made of serovars reported from marine fauna in the antarctic region from 1982-2004 with those from marine mammals in the northern hemisphere. this revealed that 10 (83%) salmonella enterica serovars isolated from antarctic penguins and seals were classifiable in high-frequency (hf) quotients for serovars prevalent in humans an ... | 2009 | 18789175 |
| lactobacillus acidophilus lap5 able to inhibit the salmonella choleraesuis invasion to the human caco-2 epithelial cell. | the mechanisms for lactic acid bacteria (lab) to inhibit salmonella invasion appear to be multifactorial and include the adhesion of lab to host intestine epithelium, the production of organic acids, or bacteriocin by lab cells. previously, we found a strain of lactobacillus acidophilus isolated from swine, i.e. strain lap5, was with antagonistic effect against salmonella typhimurium. this strain lap5 was also found to meet the requirements for probiotic use. in this study, we evaluate the poten ... | 2008 | 18789392 |
| rsta-promoted expression of the ferrous iron transporter feob under iron-replete conditions enhances fur activity in salmonella enterica. | the fur protein is a primary regulator that monitors and controls cytoplasmic iron levels. we now report the identification of a regulatory pathway mediated by the salmonella response regulator rsta that promotes fur activity. genome-wide expression experiments revealed that under iron-replete conditions, expression of the rsta protein from a plasmid lowered transcription levels of various genes involved in iron acquisition. the rsta protein controlled iron-responsive genes through the fur-fe(ii ... | 2008 | 18790861 |
| brominated furanones inhibit biofilm formation by salmonella enterica serovar typhimurium. | salmonella enterica serovar typhimurium is a main cause of bacterial food-borne diseases. as salmonella can form biofilms in which it is better protected against antimicrobial agents on a wide diversity of surfaces, it is of interest to explore ways to inhibit biofilm formation. brominated furanones, originally extracted from the marine alga delisea pulchra, are known to interfere with biofilm formation in several pathogens. in this study, we have synthesized a small focused library of brominate ... | 2008 | 18791004 |
| darkling beetles (alphitobius diaperinus) and their larvae as potential vectors for the transfer of campylobacter jejuni and salmonella enterica serovar paratyphi b variant java between successive broiler flocks. | broiler flocks often become infected with campylobacter and salmonella, and the exact contamination routes are still not fully understood. insects like darkling beetles and their larvae may play a role in transfer of the pathogens between consecutive cycles. in this study, several groups of beetles and their larvae were artificially contaminated with a mixture of salmonella enterica serovar paratyphi b variant java and three c. jejuni strains and kept for different time intervals before they wer ... | 2008 | 18791034 |
| the phoq/phop regulatory network of salmonella enterica. | the phoq/phop two-component regulatory system is a major regulator of virulence in the enteric pathogen salmonella enterica serovar typhimurium. it also controls the adaptation to low mg2+ environments by governing the expression and/or activity of mg2+ transporters and of enzymes modifying the mg2+-binding sites on the bacterial cell surface. the regulator phop modifies expression of approximately 3% of the salmonella genes in response to the periplasmic mg2+ concentration detected by the phoq ... | 2008 | 18792679 |
| allele distribution and genetic diversity of vntr loci in salmonella enterica serotype enteritidis isolates from different sources. | salmonella enterica serotype enteritidis (s. enteritidis) is a zoonotic pathogen, which can be found in many sources including animals and the environment. however, little is known about the molecular relatedness among s. enteritidis isolates from different sources. we have applied multiple-locus variable number tandem repeat analysis (mlva) to study the genetic diversity of s. enteritidis isolates from human and non-human sources. | 2008 | 18793420 |
| identification of a bile-induced exopolysaccharide required for salmonella biofilm formation on gallstone surfaces. | salmonella enterica serovar typhi can establish a chronic, asymptomatic infection of the human gallbladder, suggesting that this bacterium utilizes novel mechanisms to mediate enhanced colonization and persistence in a bile-rich environment. gallstones are one of the most important risk factors for developing carriage, and we have previously demonstrated that salmonellae form biofilms on human gallstones in vitro. thus, we hypothesize that bile-induced biofilms on gallstone surfaces promote gall ... | 2008 | 18794278 |
| identification of salmonella enterica serovar dublin-specific sequences by subtractive hybridization and analysis of their role in intestinal colonization and systemic translocation in cattle. | salmonella enterica serovar dublin is a host-restricted serovar associated with typhoidal disease in cattle. in contrast, the fowl-associated serovar s. enterica serovar gallinarum is avirulent in calves, yet it invades ileal mucosa and induces enteritis at levels comparable to those induced by s. enterica serovar dublin. suppression subtractive hybridization was employed to identify s. enterica serovar dublin strain sd3246 genes absent from s. enterica serovar gallinarum strain sg9. forty-one s ... | 2008 | 18794283 |
| antimicrobial resistance of salmonella enterica serovar typhimurium isolated from cattle in japan. | the antimicrobial susceptibility of 144 salmonella enterica serovar typhimurium isolates collected from all over japan between 1973 and 1998 were investigated. all the isolates exhibited resistance to four or more antimicrobials and 22 resistance patterns were observed. isolates showing resistance patterns to ampicillin (a), chloramphenicol (c), streptomycin (s), sulfonamides (su) and tetracycline (t), which are typical resistance patterns for s. typhimurium dt104 (dt104), were predominant. thir ... | 2008 | 18798036 |
| hild-mediated transcriptional cross-talk between spi-1 and spi-2. | the acquisition of new genetic traits by horizontal gene transfer and their incorporation into preexisting regulatory networks have been essential events in the evolution of bacterial pathogens. an example of successful assimilation of virulence traits is salmonella enterica, which acquired, at distinct evolutionary times, salmonella pathogenicity island 1 (spi-1), required for efficient invasion of the intestinal epithelium and intestinal disease, and spi-2, essential for salmonella replication ... | 2008 | 18799744 |
| salmonella enterica serovar enteritidis dam mutant induces low nos-2 and cox-2 expression in macrophages via attenuation of mapk and nf-kappab pathways. | although dam mutants of salmonella have been proposed as live vaccines, their capacity to trigger cell inflammatory cascades has not been fully elucidated. we investigated in detail the ability of salmonella enterica dam mutant to activate the signalling pathways of the inflammatory response in raw 264.7 cells. apoptosis in macrophages treated with salmonella dam mutant was low. similarly, the expression of both nos-2 and cox-2 and subsequently the production of no and pge(2) was significantly r ... | 2008 | 18801455 |
| innate immunity in caenorhabditis elegans is regulated by neurons expressing npr-1/gpcr. | a large body of evidence indicates that metazoan innate immunity is regulated by the nervous system, but the mechanisms involved in the process and the biological importance of such control remain unclear. we show that a neural circuit involving npr-1, which encodes a g protein-coupled receptor (gpcr) related to mammalian neuropeptide y receptors, functions to suppress innate immune responses. the immune inhibitory function requires a guanosine 3',5'-monophosphate-gated ion channel encoded by ta ... | 2008 | 18801967 |
| chemoenzymatic synthesis of gdp-azidodeoxymannoses: non-radioactive probes for mannosyltransferase activity. | gdp-2-, 3-, 4- or 6-azidomannoses can be successfully prepared from the corresponding azidomannose-1-phosphates and gtp using the enzyme gdp-mannosepyrophosphorylase (gdp-manpp) from salmonella enterica and may serve as useful probes for mannosyltransferase activity. | 2008 | 18802557 |
| the acquisition and internalization of salmonella by the lesser mealworm, alphitobius diaperinus (coleoptera: tenebrionidae). | in poultry broiler production facilities, it is important to understand the sources and contribution of reservoir populations of pathogens. the lesser mealworm beetle, alphitobius diaperinus (panzer), is a common pest in poultry litter that is reported to carry pathogens affecting both human and animal health. this study investigates whether the carriage of a bacterial pathogen occurs by the harboring of bacteria internally by these insects. beetles were exposed to a marker bacterium, salmonella ... | 2008 | 18803502 |
| a rapid serological assay for prediction of salmonella infection status in slaughter pigs using surface plasmon resonance. | we present a rapid surface plasmon resonance-based serological assay for the detection of salmonella typhimurium infection in pigs using the plasmonic((r)) spr device. lipopolysaccharide (lps, 10 microg ml(-1)) from salmonella typhimurium was immobilised by self-assembly on a hydrophobic spr chip. using this lps-coated chip, it was possible to bind and detect the anti-salmonella typhimurium antibodies in serum of pigs infected with the bacteria. the developed spr assay is able to differentiate b ... | 2008 | 18804126 |
| new malaria vaccine candidates based on the plasmodium vivax merozoite surface protein-1 and the tlr-5 agonist salmonella typhimurium flic flagellin. | the present study evaluated the immunogenicity of new malaria vaccine formulations based on the 19kda c-terminal fragment of plasmodium vivax merozoite surface protein-1 (msp1(19)) and the salmonella enterica serovar typhimurium flagellin (flic), a toll-like receptor 5 (tlr5) agonist. flic was used as an adjuvant either admixed or genetically linked to the p. vivax msp1(19) and administered to c57bl/6 mice via parenteral (s.c.) or mucosal (i.n.) routes. the recombinant fusion protein preserved m ... | 2008 | 18804504 |
| towards a human oral vaccine for anthrax: the utility of a salmonella typhi ty21a-based prime-boost immunization strategy. | we previously demonstrated the ability of an orally administered attenuated salmonella enterica serovar typhimurium strain expressing the protective antigen (pa) of bacillus anthracis to confer protection against lethal anthrax aerosol spore challenge [stokes mg, titball rw, neeson bn, et al. oral administration of a salmonella enterica-based vaccine expressing bacillus anthracis protective antigen confers protection against aerosolized b. anthracis. infect immun 2007;75(april (4)):1827-34]. to ... | 2008 | 18805452 |
| regulation of the salmonella enterica std fimbrial operon by dna adenine methylation, seqa, and hdfr. | dna adenine methylase (dam) mutants of salmonella enterica serovar typhimurium grown under laboratory conditions express the std fimbrial operon, which is tightly repressed in the wild type. here, we show that uncontrolled production of std fimbriae in s. enterica serovar typhimurium dam mutants contributes to attenuation in mice, as indicated by the observation that an stda dam strain is more competitive than a dam strain upon oral infection. dam methylation appears to regulate std transcriptio ... | 2008 | 18805972 |
| [construction and characterization of a novel somatostatin prokaryotic expression]. | in the current work, the fusion gene including somatostatin (ss) and the hepatitis b surface antigen gene was cloned into a balanced lethal system plasmid (pya3493), and then transformed into asd- attenuated salmonella choleraesuis c500 strain, the positive transformant without antibiotic resistance gene was confirmed by restriction analysis and dna sequencing, designated as pya-ss. the expression and immunogenicity of fusion protein were detected by sds-page and western blot analysis. these res ... | 2008 | 18807981 |
| the genome of rhodobacter sphaeroides strain 2.4.1 encodes functional cobinamide salvaging systems of archaeal and bacterial origins. | bacteria and archaea use distinct pathways for salvaging exogenous cobinamide (cbi), a precursor of adenosylcobalamin (coenzyme b(12)). the bacterial pathway depends on a bifunctional enzyme with kinase and guanylyltransferase activities (cobp in aerobic adenosylcobalamin synthesizers) to convert adenosylcobinamide (adocbi) to adocbi-guanosine diphosphate (adocbi-gdp) via an adocbi-phosphate intermediate. archaea lack cobp, and use a different strategy for the synthesis of adocbi-gdp. archaea cl ... | 2008 | 18808385 |
| molecular typing and antimicrobial resistance of salmonella enterica subspecies enterica serovar choleraesuis isolates from diseased pigs in japan. | salmonella enterica serovar choleraesuis (s. choleraesuis) isolates derived from diseased pigs in japan during 2001 and 2005 were analyzed for biotype, based on h(2)s production and dulcitol fermentation, pulsed-field gel electrophoresis (pfge) profile, and antimicrobial resistance profile. s. choleraesuis biotype choleraesuis (biotype choleraesuis) was classified into one genotype, while varietas kunzendorf (var. kunzendorf) was classified into two genotypes. the isolates of var. kunzendorf bel ... | 2010 | 18809209 |
| development of a pcr assay for the identification of salmonella enterica serovar brandenburg. | currently, salmonella enterica serovar brandenburg is identified serologically on the basis of two surface antigens, somatic (o) polysaccharide and flagellar (h) proteins. this procedure is time-consuming and requires expensive typing reagents. to overcome these problems, a pcr method was developed and validated for the identification of s. brandenburg. portions of the inva, rfbj(b), flic and fljb genes were targeted for amplification using four pairs of oligonucleotide primers. to validate the ... | 2008 | 18809549 |
| rising prevalence of enteric fever due to multidrug-resistant salmonella: an epidemiological study. | a prospective study of the prevalent aetiology of enteric fever was undertaken at a tertiary care hospital in north india at intervals of every 3 years. salmonella spp. were isolated from 174 (7%) patients. amongst these, 140 (80%) patients were infected by salmonella enterica subspecies enterica serovar typhi (s. typhi) and 16 (9%) by s. enterica serovar paratyphi a; the remaining 11% were infected by other s. enterica serogroups, typhimurium, paratyphi c and senftenberg, and other group e salm ... | 2008 | 18809553 |
| immunogenicity of recombinant attenuated salmonella enterica serovar typhimurium vaccine strains carrying a gene that encodes eimeria tenella antigen so7. | recombinant attenuated salmonella vaccines against avian coccidiosis were developed to deliver eimeria species antigens to the lymphoid tissues of chickens via the type 3 secretion system (t3ss) and the type 2 secretion system (t2ss) of salmonella. for antigen delivery via the t3ss, the eimeria tenella gene encoding sporozoite antigen so7 was cloned downstream of the translocation domain of the salmonella enterica serovar typhimurium sope gene in the parental pya3868 and pya3870 vectors to gener ... | 2008 | 18809658 |
| multiplication of salmonella enteritidis on the yolk membrane and penetration to the yolk contents at 30 degrees c in an in vitro egg contamination model. | refrigeration to limit bacterial multiplication is a critical aspect of efforts to control the transmission of salmonella enterica serovar enteritidis (se) to consumers of contaminated eggs. although the nutrient-rich yolk interior is an uncommon location for se contamination in freshly laid, naturally contaminated eggs, migration across the vitelline membrane could lead to rapid bacterial multiplication even when the initial site of deposition is outside the yolk. multiplication on the yolk mem ... | 2008 | 18810876 |
| the rate of protein secretion dictates the temporal dynamics of flagellar gene expression. | flagellar gene expression is temporally regulated in response to the assembly state of the growing flagellum. the key mechanism for enforcing this temporal hierarchy in salmonella enterica serovar typhimurium is the sigma(28)-flgm checkpoint, which couples the expression of the late flagellar (p(class3)) genes to the completion of the hook-basal body. this checkpoint is triggered when flgm is secreted from the cell. in addition to the sigma(28)-flgm checkpoint, a number of other regulatory mecha ... | 2008 | 18811728 |
| analysis of porcine transcriptional response to salmonella enterica serovar choleraesuis suggests novel targets of nfkappab are activated in the mesenteric lymph node. | specific knowledge of the molecular pathways controlling host-pathogen interactions can increase our understanding of immune response biology as well as provide targets for drug development and genetic improvement of disease resistance. toward this end, we have characterized the porcine transcriptional response to salmonella enterica serovar choleraesuis (s. choleraesuis), a salmonella serovar that predominately colonizes swine, yet can cause serious infections in human patients. affymetrix tech ... | 2008 | 18811943 |
| computational integration of structural and functional genomics data across species to develop information on the porcine inflammatory gene regulatory pathway. | we are investigating the porcine gut immune response to infection through gene expression profiling. porcine affymetrix genechip data was obtained from rna prepared from mesenteric lymph node of swine infected with either salmonella enterica serovar typhimurium (st) or s. choleraesuis (sc) for 0, 8, 24, 48 or 504 hours post-inoculation (hpi). in total, 2365 genes with statistical evidence for differential expression (de; p < 0.01, q < 0.26, fold-change > 2) between at least two time-points were ... | 2008 | 18817292 |
| transcriptional response of chicken macrophages to salmonella enterica serovar enteritidis infection. | the transcriptional profiles of chicken macrophages (hd11) infected with salmonella enterica serovar enteritidis (se) were analyzed by using an avian macrophage microarray and real time rt-pcr. out of 4906 array elements interrogated, 269 genes exhibited a 2 fold change (p < 0.001) over a 24 h time-course. genes coding for proinflammatory cytokines, cc and cxc chemokines, and chemokine ligand were upregulated; whereas genes associated with transcription, cell adhesion and proliferation were down ... | 2008 | 18817296 |
| dynamic remodeling of the endosomal system during formation of salmonella-induced filaments by intracellular salmonella enterica. | the infection by salmonella enterica results in the massive remodeling of the endosomal system of eukaryotic host cells. one unique consequence is the formation of long tubular endosomal compartments, so-called salmonella-induced filaments (sif). formation of sif requires the function of type iii secretion system and is a requirement of efficient intracellular proliferation of salmonella. using high-resolution live cell imaging approaches and electron microscopy, we report for the first time the ... | 2008 | 18817527 |
| binding of the unorthodox transcription activator, crl, to the components of the transcription machinery. | the small regulatory protein crl binds to sigmas, the rna polymerase stationary phase sigma factor. crl facilitates the formation of the sigmas-associated holoenzyme (esigmas) and thereby activates sigmas-dependent genes. using a real time surface plasmon resonance biosensor, we characterized in greater detail the specificity and mode of action of crl. crl specifically forms a 1:1 complex with sigmas, which results in an increase of the association rate of sigmas to core rna polymerase without a ... | 2008 | 18818199 |
| smva, and not acrb, is the major efflux pump for acriflavine and related compounds in salmonella enterica serovar typhimurium. | the aim was to study the role played by smva pump in the efflux of quaternary ammonium compounds (qacs) in salmonella enterica serovar typhimurium (salmonella typhimurium). | 2008 | 18819967 |
| characterization of a t7-like lytic bacteriophage (phisg-jl2) of salmonella enterica serovar gallinarum biovar gallinarum. | phisg-jl2 is a newly discovered lytic bacteriophage infecting salmonella enterica serovar gallinarum biovar gallinarum but is nonlytic to a rough vaccine strain of serovar gallinarum biovar gallinarum (sg-9r), s. enterica serovar enteritidis, s. enterica serovar typhimurium, and s. enterica serovar gallinarum biovar pullorum. the phisg-jl2 genome is 38,815 bp in length (gc content, 50.9%; 230-bp-long direct terminal repeats), and 55 putative genes may be transcribed from the same strand. functio ... | 2008 | 18820072 |
| nationwide outbreak of salmonella enterica serotype gives infections in infants in france, linked to infant milk formula, september 2008. | 2008 | 18822245 | |
| [a novel salmonella typhimurium plasmid, panks: an example for plasmid evolution in antibiotic resistance]. | in this study, a plasmid, carrying ampicillin resistance (ampr) gene, isolated from a clinical isolate of salmonella enterica serotype typhimurium presenting acssut (ampicilin, chloramphenicol, streptomycin, sulphonamide, tetracycline) resistance phenotype, was defined. the length of complete sequence of this plasmid was 8271 base pairs (bp), and it was named as panks owing to its isolation place (plasmid-ankara- salmonella). the plasmid was analyzed for potential reading frames and structural f ... | 2008 | 18822880 |
| salmonella infection of a penile prosthesis. | patients with erectile dysfunction (ed) who have failed oral or intracavernosal erectogenic therapy may benefit from inflatable penile prosthesis (ipp) placement. such individuals commonly include patients with diabetes mellitus or chronic renal insufficiency. complications of ipp placement include erosion and infection. most often, infection of ipps involves skin flora that are hypothesized to be introduced at the time of device placement and persist as a biofilm, causing clinical infection as ... | 2009 | 18823319 |
| salmonella indiana as a cause of abortion in ewes: genetic diversity and resistance patterns. | salmonella enterica subspecies enterica indiana, a food-borne serovar uncommon in most countries, was responsible for an outbreak of abortion in a flock of lacaune dairy ewes in southern spain. drinking water and feedstuff samples were analysed in an attempt to determine the source of the infection. pigeons (columba livia) and turtledoves (streptopelia turtur) in close contact with the ewes were captured and examined for the bacterium. seventeen s. indiana strains were isolated from the ewes and ... | 2009 | 18823722 |
| synthesis and recovery of high bioactive phenolics from table-olive brine process wastewater. | the generated wastewater of table-olive brine processing contains a high amount of polyphenols which are endowed with interesting biological activities. the ethyl acetate extract (eae) of such wastewater shows high hydroxytyrosol (ht) and tyrosol concentrations of 690 and 98 mg g(-1) dry weight extract, respectively. phenolic compounds analysis was performed by gas chromatography-mass spectrometry (gc-ms). the antioxidant activity was evaluated by the scavenging effect on dpph (2,2-diphenyl-1-pi ... | 2008 | 18823782 |
| 'form variation' of the o12 antigen is critical for persistence of salmonella typhimurium in the murine intestine. | salmonella enterica subspecies i serotypes are responsible for the vast majority of salmonellosis in mammals and birds, yet only a few factors specific to this group that allow them to persist in this niche have been identified. we show that stm0557, a s. enterica subspecies i-specific gene encoding an inner membrane protein, is critical for faecal shedding and intestinal persistence of s. enterica serotype typhimurium atcc14028 in salmonella-resistant mice, but mutations in this gene do not dim ... | 2008 | 18826410 |
| expression of antimicrobial peptides in cecal tonsils of chickens treated with probiotics and infected with salmonella enterica serovar typhimurium. | several strategies currently exist for control of salmonella enterica serovar typhimurium colonization in the chicken intestine, among which the use of probiotics is of note. little is known about the underlying mechanisms of probiotic-mediated reduction of salmonella colonization. in this study, we asked whether the effect of probiotics is mediated by antimicrobial peptides, including avian beta-defensins (also called gallinacins) and cathelicidins. four treatment groups were included in this s ... | 2008 | 18827189 |
| prevalence and characterization of salmonella spp. among marine animals in the channel islands, california. | salmonella enterica is a zoonotic pathogen that has been isolated from free-ranging marine mammals throughout the world, with animals in the channel islands of california (usa) showing the highest prevalence. the goal of this study was to determine prevalence, antimicrobial sensitivity and genetic similarity using pulsed-field gel electrophoresis (pfge) of salmonella in several non-domestic animal species on san miguel and san nicolas islands. fecal samples were collected from 90 california sea ... | 2008 | 18828559 |
| enhanced bactericidal effect of enterocin as-48 in combination with high-intensity pulsed-electric field treatment against salmonella enterica in apple juice. | the effect of the broad spectrum cyclic antimicrobial peptide enterocin as-48 combination with high-intensity pulsed-electric field (hipef) treatment (35 kv/cm, 150 hz, 4 micros and bipolar mode) was tested on salmonella enterica cect 915 in apple juice. a response surface methodology was applied to study the bactericidal effects of the combined treatment. the process variables were as-48 concentration, temperature, and hipef treatment time. while treatment with enterocin as-48 alone up to 60 mi ... | 2008 | 18829125 |
| interaction of salmonella enterica with basil and other salad leaves. | contaminated salad leaves have emerged as important vehicles for the transmission of enteric pathogens to humans. a recent outbreak of salmonella enterica serovar senftenberg (s. senftenberg) in the united kingdom has been traced to the consumption of contaminated basil. using the outbreak strain of s. senftenberg, we found that it binds to basil, lettuce, rocket and spinach leaves showing a pattern of diffuse adhesion. flagella were seen linking s. senftenberg to the leaf epidermis, and the del ... | 2009 | 18830276 |
| reduction in infection risk through treatment of microbially contaminated surfaces with a novel, portable, saturated steam vapor disinfection system. | surface-mediated infectious disease transmission is a major concern in various settings, including schools, hospitals, and food-processing facilities. chemical disinfectants are frequently used to reduce contamination, but many pose significant risks to humans, surfaces, and the environment, and all must be properly applied in strict accordance with label instructions to be effective. this study set out to determine the capability of a novel chemical-free, saturated steam vapor disinfection syst ... | 2009 | 18834748 |
| a novel dna microarray for rapid diagnosis of enteropathogenic bacteria in stool specimens of patients with diarrhea. | a microarray technique for the detection and identification of enteropathogenic bacteria at the species and subspecies levels was developed in this study, and the target bacteria included pathogenic escherichia coli, vibrio cholerae, vibrio parahaemolyticus, salmonella enterica, campylobacter jejuni, shigellae, yersinia enterocolitica, and listeria monocytogenes. the virulence gene of each pathogen was chosen as the amplification target, labeled with a fluorescence dye by multiplex polymerase ch ... | 2008 | 18834908 |
| recombinant spao and h1a as immunogens for protection of mice from lethal infection with salmonella paratyphi a: implications for rational design of typhoid fever vaccines. | the vi capsular polysaccharide vaccine is one of two vaccines against typhoid recommended worldwide and is the vaccine generally used in china. however, in recent years a salmonella paratyphi a strain that is naturally devoid of capsule has caused frequent outbreaks of typhoid fever in southern china, leading to the need for identification of additional antigens that could be incorporated into new vaccines. spao acts as a major invasion factor of salmonella enterica spp. and h1a is the unique fl ... | 2008 | 18834913 |
| modulation of macrophage proinflammatory functions by cytokine-expressing salmonella vectors. | we previously reported that the intraperitoneal administration of recombinant strains of salmonella enterica serovar typhimurium, engineered to express murine il-2 (designated gidil2) or ifn-gamma (gidifngamma), induced a cytokine-specific modulation of the host innate immune response. interestingly, the bacteria-expressed cytokines were not secreted, but instead were associated with the bacterial cytosol. to understand the mechanism by which these two transfectants influence immune cells, we in ... | 2009 | 18835224 |
| predicting salmonella enterica serotypes by repetitive sequence-based pcr. | repetitive extragenic palindromic sequence-based pcr (rep-pcr) utilizing a semi-automated system, was evaluated as a method to determine salmonella serotypes. a group of 216 salmonella isolates belonging to 13 frequently isolated serotypes and one rarer serotype from poultry were used to create a dna fingerprint library with the diversilab system software. subsequently, a blinded set of 44 poultry isolates were fingerprinted and queried against the library in an attempt to putatively assign a se ... | 2009 | 18835303 |
| fluorescent bio-barcode dna assay for the detection of salmonella enterica serovar enteritidis. | salmonella enterica serovar enteritidis is one of the most frequently reported causes of foodborne illness. it is a major threat to the food safety chain and public health. a highly amplified bio-barcode dna assay for the rapid detection of the insertion element (iel) gene of salmonella enteritidis is reported in this paper. the biosensor transducer is composed of two nanoparticles: gold nanoparticles (au-nps) and magnetic nanoparticles (mnps). the au-nps are coated with the target-specific dna ... | 2009 | 18835708 |
| analysis of yggx and gsha mutants provides insights into the labile iron pool in salmonella enterica. | strains of salmonella enterica lacking yggx and the cellular reductant glutathione exhibit defects similar to those resulting from iron deficiency and oxidative stress. mutant strains are sensitive to hydrogen peroxide and superoxide, deregulate the expression of the fur-regulated gene entb, and fail to grow on succinate medium. suppression of some yggx gsha mutant phenotypes by the cell-permeable iron chelator deferoxamine allowed the conclusion that increased levels of cellular fenton chemistr ... | 2008 | 18835989 |
| [identification of genes for biofilm formation in a salmonella enteritidis strain by transposon mutagenesis]. | biofilm plays an important role in pathogenicity of salmonella and food poisoning caused by salmonella. our aim was to identify genes associated with salmonella biofilm formation. | 2008 | 18837362 |
| early cellular responses to salmonella infection: dendritic cells, monocytes, and more. | summary: dendritic cells (dcs), monocytes, macrophages, and neutrophils are myeloid-derived phagocytes critical to controlling bacterial infections, and these cells have complementary functions to ensure host survival. recent data have shed light on the dynamics and function of myeloid cells at the early stage of infection. in particular, murine infection models with salmonella enterica serovar typhimurium have been useful for understanding the host response required to develop immunity to syste ... | 2008 | 18837781 |
| adrenaline modulates the global transcriptional profile of salmonella revealing a role in the antimicrobial peptide and oxidative stress resistance responses. | the successful interaction of bacterial pathogens with host tissues requires the sensing of specific chemical and physical cues. the human gut contains a huge number of neurons involved in the secretion and sensing of a class of neuroendocrine hormones called catecholamines. recently, in escherichia coli o157:h7, the catecholamines adrenaline and noradrenaline were shown to act synergistically with a bacterial quorum sensing molecule, autoinducer 3 (ai-3), to affect bacterial virulence and motil ... | 2008 | 18837991 |
| microflora of bile aspirates in patients with acute cholecystitis with or without cholelithiasis: a tropical experience. | the current study determined the spectrum of biliary microflora with special emphasis on enteric fever organisms in patients with acute cholangitis with and without cholelithiasis or other biliary diseases. the patients were divided into three groups: group a consisted of patients with acute cholecystitis with cholelithiasis; group b consisted of patients with acute cholecystitis with gastrointestinal ailments requiring biliary drainage and group c consisted of patients with gallbladder carcinom ... | 2008 | 18839486 |
| comparison of classical serotyping and premitest assay for routine identification of common salmonella enterica serovars. | the commercial premitest salmonella kit uses a multiplexed dna typing test aimed at identifying common serovars of salmonella enterica. it was used in assays over a 9-month period in the belgian reference laboratory that performs the routine identification of salmonella strains of animal origin. a blind analysis of 754 strains was conducted in parallel by classical serotyping and the premitest assay. full results were available for 685 strains (90.8%) by serotyping, while the remaining 69 strain ... | 2008 | 18842945 |
| azithromycin for treating uncomplicated typhoid and paratyphoid fever (enteric fever). | enteric fever (typhoid and paratyphoid fever) is potentially fatal. infection with drug-resistant strains of the causative organism salmonella enterica serovar typhi or paratyphi increases morbidity and mortality. azithromycin may have better outcomes in people with uncomplicated forms of the disease. | 2008 | 18843701 |
| rapid screening of salmonella enterica serovars enteritidis, hadar, heidelberg and typhimurium using a serologically-correlative allelotyping pcr targeting the o and h antigen alleles. | classical salmonella serotyping is an expensive and time consuming process that requires implementing a battery of o and h antisera to detect 2,541 different salmonella enterica serovars. for these reasons, we developed a rapid multiplex polymerase chain reaction (pcr)-based typing scheme to screen for the prevalent s. enterica serovars enteritidis, hadar, heidelberg, and typhimurium. | 2008 | 18845003 |
| potent anti-tumor activity of systemically-administered il2-expressing salmonella correlates with decreased angiogenesis and enhanced tumor apoptosis. | salmonella enterica serovar typhimurium (hereafter s. typhimurium) stains have been shown to exert a potent inhibitory effect on the growth of human and mouse tumors in experimental models. our laboratory has previously demonstrated that an attenuated strain of s. typhimurium engineered to express il2 (designated strain gidil2) has demonstrable immunopotentiating properties, particularly affecting the innate arm of the immune system. in the present study, we wished to explore the properties of i ... | 2009 | 18849195 |
| characterization of the genomes of a diverse collection of salmonella enterica serovar typhimurium definitive phage type 104. | salmonella enterica serovar typhimurium definitive phage type 104 (dt104) has caused significant morbidity and mortality in humans and animals for almost three decades. we completed the full dna sequence of one dt104 strain, nctc13348, and showed that significant differences between the genome of this isolate and the genome of the previously sequenced strain salmonella serovar typhimurium lt2 are due to integrated prophage elements and salmonella genomic island 1 encoding antibiotic resistance g ... | 2008 | 18849424 |
| bacterial flagella are firmly anchored. | there are mutants of salmonella enterica (with mutations in flif and flil) that shed flagella when they are swimming in a viscous medium or on the surface of soft agar. filaments with hooks and the distal rod segment flgg are recovered. we tried to extract flagellar filaments from such cells by pulling on them with an optical trap but failed, even when we used forces large enough to straighten the filaments. thus, flagella are firmly anchored. | 2008 | 18849436 |
| packed with salmonella--investigation of an international outbreak of salmonella senftenberg infection linked to contamination of prepacked basil in 2007. | salmonella senftenberg is uncommon in the united kingdom. in january-june 2007, the health protection agency reported on 55 primary human cases of salmonella senftenberg in england and wales. in may 2007, fresh basil sold in the united kingdom was found to be contaminated with salmonella senftenberg. we launched an investigation to elucidate the cause of this outbreak. isolates were examined using plasmid profiling and pulsed-field gel electrophoresis, and the outbreak strain (ssftxb.0014) was i ... | 2008 | 18851676 |
| distribution of multidrug-resistant human isolates of mdr-acssut salmonella typhimurium and mdr-ampc salmonella newport in the united states, 2003-2005. | multidrug-resistant (mdr) salmonella strains are associated with excess bloodstream infections, hospitalizations, and deaths compared with pansusceptible strains. bovine products are sometimes a source of mdr salmonella. to generate hypotheses for regional differences in risk factors for human infection, we analyzed distributions of the two most prevalent mdr salmonella phenotypes in the united states, 2003-2005: (i) mdr-acssut (resistant to at least ampicillin, chloramphenicol, streptomycin, su ... | 2008 | 18851677 |
| impact of prior immunological exposure on vaccine delivery by salmonella enterica serovar typhimurium. | recombinant salmonella have been employed as vaccine vectors to deliver dna and protein vaccines of viral, bacterial and parasitic origin. however, the effectiveness of salmonella delivery may be hampered by prior immunological exposure to salmonella. we investigated the effects of prior exposure to the salmonella typhimurium aroad strain brd509 on its ability to deliver the non-toxic c fragment of tetanus toxin (tt). balb/c mice were orally immunised with brd509 containing the c fragment expres ... | 2008 | 18852007 |
| characterization of puo-stvr2, a virulence-resistance plasmid evolved from the pslt virulence plasmid of salmonella enterica serovar typhimurium. | puo-stvr2 is a virulence-resistance plasmid which originated from pslt of salmonella enterica serovar typhimurium through acquisition of a complex resistance island, flanked by regions that provide a toxin-antitoxin system and an iron uptake system. the presence of resistance and virulence determinants on the same plasmid allows coselection of both properties, potentially increasing health risks. | 2008 | 18852276 |