Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| current status and prospects on microbial control in japan. | historically in japan, studies on the diseases of the silkworm, bombyx mori, as a factor affecting the well-being of the silk industry, have dominated insect pathology. however, work by hidaka in 1933 demonstrated the possibility of controlling the pine moth, dendrolimus spectabilis, with the fungus beauveria bassiana and since then, various attempts have been made to develop a method to control insect pests using insect pathogens. the cypovirus product, matsukemin, was the first microbial contr ... | 2007 | 17462666 |
| nonribosomal peptide synthesis in aspergillus fumigatus and other fungi. | in fungi, nonribosomal peptide synthetases (nrp synthetases) are large multi-functional enzymes containing adenylation, thiolation (or peptidyl carrier protein, pcp) and condensation domains. these enzymes are often encoded within gene clusters. multiple nrp synthetase orfs have also been identified in fungi (14 in aspergillus fumigatus). leaa, a methyltransferase, is involved in secondary metabolite gene cluster regulation in aspergillus spp. the nrp synthetases glip and ftma respectively direc ... | 2007 | 17464044 |
| directed evolution for increased chitinase activity. | directed evolution through dna shuffling and screening was used to enhance the catalytic ability of a fungal, beauveria bassiana, chitinase, bbchit1. the bbchit gene was first linked to various prokaryotic signal sequences and expressed in escherichia coli. the signal peptide, pelb, from erwinia carotovora resulted in greatest chitinase secretion into broth. the nucleotide sequence expressing pelb signal peptide was then incorporated into an e. coli vector to express bbchit1 variants generated b ... | 2007 | 17468866 |
| [molecular evaluation on the efficacy of sustainable control of monochamus alternatus by non-woven fabric fungal bands impregnated with beauveria bassiana]. | by using molecular marker of group i intron, a field evaluation was made on the efficacy of sustainable control of monochamus alternatus by non-woven fabric bands impregnated with beauveria bassiana. the results showed that the control efficacy in two treated plots was 19.5% and 10.8%, and 14.2% and 11% when evaluated with conventional and molecular evaluation methods, respectively, suggesting that the conventional method which evaluated the control efficacy in terms of microbes mortality or inf ... | 2007 | 17552194 |
| a tritrophic effect of host plant on susceptibility of western flower thrips to the entomopathogenic fungus beauveria bassiana. | adult female western flower thrips (frankliniella occidentalis) were exposed 12-24h to bean (phaseolus vulgaris) and impatiens (impatiens wallerana) leaf disks treated with beauveria bassiana conidia and then transferred to clean bean or impatiens at various times post-treatment. significantly greater levels of fungal infection were observed when thrips were treated on bean versus impatiens, but exposure to impatiens following treatment had no effect on fungal infection (percent mortality). this ... | 2007 | 17572438 |
| [efficiency of beauveria bassiana and metarhizium anisopliae formulations on amblyomma cajennense (fabricius, 1787) nymphae]. | amblyomma cajennense is a vector of several infectious diseases on mammals and became a serious problem in some localities in brazil. entomopathogenic fungi are very important biocontrol agents and could be a viable alternative for the control of this tick in permanent preserved areas. the aim of this study was to evaluated the mortality of tick nymphae treated with beauveria bassiana and metarhizium anisopliae formulations in laboratory conditions. non fed nymphae were inoculated with 2x10(7) a ... | 2007 | 17588319 |
| comparative evaluation and economic potential of ecorational versus chemical insecticides for crucifer flea beetle (coleoptera: chrysomelidae) management in canola. | from 2001 to 2004, field studies were conducted to evaluate the effect of the ecorational insecticides spintor (spinosad), botanigard (beauveria bassiana), neemix (azadirachtin), and surround (kaolin) against crucifer flea beetle on canola, brassica napus l., at the cotyledon stage. the ecorational treatments were compared with a standard foliar chemical insecticide, capture (bifenthrin), and the chemical seed treatment insecticide helix xtra (thiamethoxam). this study indicated that flea beetle ... | 2007 | 17598529 |
| inoculation of coffee plants with the fungal entomopathogen beauveria bassiana (ascomycota: hypocreales). | the entomopathogenic fungus beauveria bassiana was established in coffee seedlings after fungal spore suspensions were applied as foliar sprays, stem injections, or soil drenches. direct injection yielded the highest post-inoculation recovery of endophytic b. bassiana. establishment, based on percent recovery of b. bassiana, decreased as time post-inoculation increased in all treatments. several other endophytes were isolated from the seedlings and could have negatively influenced establishment ... | 2007 | 17604149 |
| variability in tolerance to uv-b radiation among beauveria spp. isolates. | solar radiation, particularly the uv-b component, negatively affects survival of entomopathogenic fungi in the field. in an effort to identify beauveria spp. isolates with promise for use in biological control settings with high insolation, we examined 53 beauveria bassiana isolates, 7 isolates of 4 other beauveria spp. and engyodontium albus (=beauveria alba). the origins of these fungi varied widely as to host/substrate and country, but approximately 30% of these isolates were b. bassiana from ... | 2007 | 17610892 |
| [pathogenicity of several fungal species on spodoptera litura]. | the virulence test of five species of entomogenous fungi beauveria brongniartii, beauveria bassiana, paecilomyces fumosoroseus, metarhizium anisopliae and nomuraea rileyi to spodoptera litura larvae showed that b. brongniartii and n. rileyi had evident pathogenic effects on s. litura, with the lt50 value to s. litura 2nd instars being 2.95 and 4.10 days, and the corrected accumulative mortality of the instars being 100% and 95.2%, respectively. the virulence of b. brongniartii and n. rileyi to t ... | 2007 | 17615897 |
| control of the sheep scab mite psoroptes ovis in vivo and in vitro using fungal pathogens. | as part of a research programme designed to identify biological agents for the control of sheep scab, the pathogenicity of the fungus metarhizium anisopliae to psoroptes mites in the presence of sheepskin and wool was examined in the laboratory. no inhibitory effects of skin and wool were observed and high levels of infection were recorded. subsequently the pathogenicity of formulations of both m. anisopliae and beauveria bassiana to psoroptes ovis was studied in vivo. for this, 36 batches of 20 ... | 2007 | 17624674 |
| human exposure to airborne fungi from genera used as biocontrol agents in plant production. | the fungi trichoderma harzianum, t. polysporum, t. viride, paeciliomyces fumosoroseus, p. lilacinus, verticillium/lecanicillium lecanii, ulocladium oudemansii, u. atrum and beauveria bassiana are used or considered to be used for biocontrol of pests and plant diseases. human exposure to these fungi in environments where they may naturally occur or are used as biocontrol agents has not been directly investigated to date. this review aims to provide an overview of the current knowledge of human ex ... | 2007 | 17655172 |
| broad-spectrum antimicrobial activity of the reactive compounds generated in vitro by manduca sexta phenoloxidase. | although quinone production and melanin formation are widely recognized as an integral part of the insect defense system, experimental evidence is lacking that the proteolytic activation of prophenoloxidase participates in the direct killing of invading microbes-active phenoloxidase generates quinones that polymerize to form melanin. here, we report the antimicrobial effect of reactive intermediates produced in phenoloxidase-catalyzed reactions. after being treated with manduca sexta phenoloxida ... | 2007 | 17681234 |
| expression of a beauveria bassiana chitinase (bbchit1) in escherichia coli and pichia pastoris. | beauveria bassiana chitinase (bbchit1) is an important cuticle degrading enzyme involved in pathogenesis of fungi against insect. to obtain enough active chitinase for performing in vitro functional analysis, bbchit1 gene was expressed in escherichia coli and pichia pastoris, respectively. the high-level production of recombinant bbchit1 was detected in e. coli expression system, however mainly located in inclusion bodies. refolding of solubilized inclusion body proteins was achieved by dialysis ... | 2007 | 17692532 |
| recalcitrant beauveria bassiana keratitis: confocal microscopy findings and treatment with posaconazole (noxafil). | to report the clinical, confocal microscopic, and histologic appearance of a case of beauveria bassiana keratitis and response to medical therapy. | 2007 | 17721308 |
| analysis of differential gene expression in the generalist entomopathogenic fungus beauveria bassiana (bals.) vuillemin grown on different insect cuticular extracts and synthetic medium through cdna-aflps. | the entomopathogen beauveria bassiana has an extremely diverse insect host range in addition to a saprophytic phase. the molecular basis for this plasticity was investigated by a comparative analysis of gene expression patterns through cdna-aflps of a b. bassiana isolate grown on cuticular extracts of insects of diverse orders as well as synthetic medium. for some of the fragments, expression patterns were verified by q-rt-pcr. among the differentially expressed genes two proteases were identifi ... | 2007 | 17723310 |
| factors affecting the occurrence and distribution of entomopathogenic fungi in natural and cultivated soils. | factors affecting the occurrence and distribution of entomopathogenic fungi in 244 soil samples collected from natural and cultivated areas in spain were studied using an integrated approach based on univariate and multivariate analyses. entomopathogenic fungi were isolated from 175 of the 244 (71.7%) soil samples, with only two species found, beauveria bassiana and metarhizium anisopliae. of the 244 soil samples, 104 yielded b. bassiana (42.6%), 18 yielded m. anisopliae (7.3%), and 53 soil samp ... | 2007 | 17766099 |
| cytotoxic and antihaptotactic beauvericin analogues from precursor-directed biosynthesis with the insect pathogen beauveria bassiana atcc 7159. | precursor-directed biosynthesis was used to produce analogues of the cyclic depsipeptide mycotoxin beauvericin (1) using the filamentous fungus beauveria bassiana atcc 7159. feeding 30 analogues of d-2-hydroxyisovalerate and l-phenylalanine, the natural 2-hydroxycarboxylic acid and amino acid precursors of beauvericin, led to the biosynthesis of novel beauvericins. six of these were isolated and characterized, and their cytotoxicity and directional cell migration (haptotaxis) inhibitory activity ... | 2007 | 17803266 |
| [studies on purification and properties of antagonistic protein from bacteria ss02 of paenibacillus daejeonensis]. | the antifungal, anti-bacterical, anti-brine shrimp activities of sd22 isolated from paenibacillus daejeonensis bacteria ss02 were studied. the separation steps included ultracentrifugation, ultrafiltration and (nh4)2so4 fractional precipitation, further purification was performed by sephadexg-75 and deae-32 chromatography. its molecular weight determined by sds-page was 56.0 kd and its isoelectfic point was 6.4. sd22 was thermostable to some extent and stable to ultraviolet, but sensitive to som ... | 2007 | 17822044 |
| enhanced efficacy of beauveria bassiana for red flour beetle with reduced moisture. | red flour beetle, tribolium castaneum (herbst), is a major pest of stored and processed grains that is tolerant of beauveria bassiana (balsamo) vuillemin under most conditions. laboratory assays demonstrated that desiccating conditions improved the efficacy of the fungus. for t. castaneum larvae exposed to b. bassiana at different vapor pressure deficits (vpds), the lc50 potency ratios were 2.3 for 2.42 kpa and 3.9 for 3.31 kpa compared with 1.06 kpa. there were significant effects of moisture o ... | 2007 | 17849853 |
| comparisons of ecorational and chemical insecticides against crucifer flea beetle (coleoptera: chrysomelidae) on canola. | studies were done comparing the insecticides spintor (spinosad), botanigard (beauveria bassiana), neemix (azadirachtin), and surround (kaolin, a clay) with conventional chemical insecticides capture (bifenthrin) and helix xtra (thiamethoxam), all applied at seven rates in a 7-d assay to assess their impact on adult crucifer flea beetle, phyllotreta cruciferae (goeze) (coleoptera: chrysomelidae). lethal time (lt) required for 25, 50, and 75% mortality (lt25, lt50, and lt75) and relative potencies ... | 2007 | 17849871 |
| natural occurrence of beauveria bassiana in hypothenemus hampei (coleoptera: curculionidae) populations in unsprayed coffee fields. | three unsprayed coffee farms (farm 1, 2 and 3) were studied for the natural occurrence of the insect pathogenic fungus beauveria bassiana in hypothenemus hampei populations throughout the rainy season of 2004 (july-november) and 2005 (july-december). b. bassiana infections were found during most sampling dates in both years, on all three farms. the b. bassiana infection levels were higher in 2005 than in 2004 with mean prevalence of 12.1% and 2.7%, respectively. no consistent significant differe ... | 2008 | 17854825 |
| selection of beauveria bassiana isolates to control alphitobius diaperinus. | this paper presents results on isolates of the entomopathogenic fungus beauveria bassiana for alphitobius diaperinus (coleoptera: tenebrionidae) control. of the 30 isolates tested, four (cg 71, cg 152, unioeste 4, and unioeste 40) resulted in mortality rates >or= 40% within 10 days. these mortality rates could be considered high because of the resistance of these species to b. bassiana. tests were conducted using these isolates to estimate lc(50), mortality rate over time, vegetative growth, and ... | 2008 | 17869265 |
| new feather-degrading filamentous fungi. | among 106 filamentous fungi isolated from poultry farm waste, 13 species belonging to seven genera (aspergillus, acremonium, alternaria, beauvaria, curvularia, paecilomyces, and penicillium) were able to grow and produce keratinase in stationary cultures using poultry feather powder as the only substrate. the four most efficient keratinase producers were selected for a comparative study of keratinase production in submerged and stationary conditions. the highest keratinolytic activities were pro ... | 2008 | 17899245 |
| phage display cdna cloning and expression analysis of hydrophobins from the entomopathogenic fungus beauveria (cordyceps) bassiana. | hydrophobins are small amphipathic proteins that function in a broad range of growth and developmental processes in fungi. they are involved in the formation of aerial structures, the attachment of fungal cells to surfaces, and act in signalling in response to surface cues and pathogenesis. beauveria bassiana is an important entomopathogenic fungus used as an arthropod biological control agent. to examine the feasibility of using phage display technology to clone cdnas encoding hydrophobins, bio ... | 2007 | 17906142 |
| phage display cdna cloning and expression analysis of hydrophobins from the entomopathogenic fungus beauveria (cordyceps) bassiana. | hydrophobins are small amphipathic proteins that function in a broad range of growth and developmental processes in fungi. they are involved in the formation of aerial structures, the attachment of fungal cells to surfaces, and act in signalling in response to surface cues and pathogenesis. beauveria bassiana is an important entomopathogenic fungus used as an arthropod biological control agent. to examine the feasibility of using phage display technology to clone cdnas encoding hydrophobins, bio ... | 2007 | 17906142 |
| surface characteristics of the entomopathogenic fungus beauveria (cordyceps) bassiana. | marked differences in surface characteristics were observed among three types of single-cell propagules produced by the entomopathogenic fungus beauveria bassiana. atomic force microscopy (afm) revealed the presence of bundles or fascicles in aerial conidia absent from in vitro blastospores and submerged conidia. contact angle measurements using polar and apolar test liquids placed on cell layers were used to calculate surface tension values and the free energies of interaction of the cell types ... | 2007 | 17906143 |
| surface characteristics of the entomopathogenic fungus beauveria (cordyceps) bassiana. | marked differences in surface characteristics were observed among three types of single-cell propagules produced by the entomopathogenic fungus beauveria bassiana. atomic force microscopy (afm) revealed the presence of bundles or fascicles in aerial conidia absent from in vitro blastospores and submerged conidia. contact angle measurements using polar and apolar test liquids placed on cell layers were used to calculate surface tension values and the free energies of interaction of the cell types ... | 2007 | 17906143 |
| ovicidal activity of entomopathogenic hyphomycetes on aedes aegypti (diptera: culicidae) under laboratory conditions. | the ovicidal activity of 21 hyphomycete fungi species against aedes aegypti (l.) (diptera: culicidae) was tested. fungi with ovicidal activity developed on high numbers of eggs (> or =70%) during 25 d of exposure. a clear ovicidal activity with low values of hatch (1.3-40%) was observed after 25 d of incubation with isaria farinosa (holm: fries) fries, paecilomyces carneus (duché & heim) brown & smith, paecilomyces marquandii (massee) hughes, isaria fumosorosea (wize), metarhizium anisopliae (me ... | 2007 | 17915511 |
| populational parameters of spalangia endius walker (hymenoptera: pteromalidae) on pupae of musca domestica l. (diptera: muscidae) treated with two strains of beauveria bassiana (bals.) vuil. (deuteromycetes). | the parasitoid spalangia endius walker is an efficient controller of dipteran pupae, such as musca domestica l. the entomopathogenic fungus beauveria bassiana (bals.) vuil. is a regulator of insect populations, including these synanthropic pests. the aim of this work was to explore the possibilities of utilizing both agents in a combined form for the biocontrol of the domestic fly. recently formed m. domestica pupae were inoculated by immersion in conidia suspension (10(8) conidia/ml) with two s ... | 2007 | 17934618 |
| biocontrol of almond bark beetle (scolytus amygdali geurin-meneville, coleoptera: scolytidae) using beauveria bassiana (bals.) vuill. (deuteromycotina: hyphomycetes). | to formulate the entomopathogenic fungus beauveria bassiana in invert emulsion, then apply it against adults of almond bark beetle (scolytus amygdali) under laboratory and field conditions. | 2007 | 17953551 |
| isolation and characterization of nucleotide excision repair deficient mutants of the entomopathogenic fungus, beauveria bassiana. | to better understand dna repair in the entomopathogenic fungus beauveria bassiana, three ultraviolet (uv) light sensitive mutants were isolated and characterized to be deficient in nucleotide excision repair (ner). the uv sensitive mutants were scored by comparison to survival of the parental isolate, gk2016, after 36 j/m(2) uv-c irradiation. at this dose, conidial survival of gk2016 was 98% and the mutants lc75, lc194, and lc85 had survival values of 63%, 45%, and 31%, respectively. an immunolo ... | 2008 | 18005981 |
| means to mediating accumulation of hydrophobin-like proteins in the wall of beauveria bassiana conidia for improved tolerance to thermal stress. | 2007 | 18057822 | |
| [species diversity and seasonal change of entomogenous fungi in langya mountains nature reserve]. | 672 specimens of entomogenous fungi belonging to 20 species, 8 genera and 4 families were collected from 20 sampling quadrates in the langya mountains nature reserve of east anhui. the dominant species were beauveria bassiana, paecilomyces farinosus, p. cateinannulatus, b. brongniartii and p. fumosoroseus, and b. bassiana was most dominant, with the relative abundance of 73.8%. the entomogenous fungi in the reserve were high in amount but low in diversity. the amount of isolates and the abundanc ... | 2007 | 18062316 |
| production and regulation of cuticle-degrading proteases from beauveria bassiana in the presence of rhammatocerus schistocercoides cuticle. | extracellular proteases have been shown to be virulence factors in fungal pathogenicity toward insects. we examined the production of extracellular proteases, subtilisin-like activity (pr1) and trypsin-like activity (pr2), by beauveria bassiana cg425, which is a fungus of interest for control of the grasshopper rhammatocerus schistocercoides. to access the role of these proteases during infection of r. schistocercoides, we analyzed their secretion during fungus growth either in nitrate-medium or ... | 2008 | 18071791 |
| [development of the fungi metarhizium anisopliae (metschnikoff, 1879) sorokin, 1883 and beauveria bassiana (balsamo) vuillemin, 1912 on the ctenophephalides felis felis (bouché, 1835)]. | the flea ctenocephalides felis felis is a parasite that causes allergic dermatitis and also may transmit etiologic agents to domestic animals and humans. this study investigated by scanning electron microscopy the development of entomopathogenic fungi on flea cuticle. fleas were exposed to conidia (10(8) ml(-1)) of metarhizium anisopliae (isolate 959) or beauveria bassiana (isolate 986). following standard protocols for electron microscopy, the specimens were prepared 2, 15, 26 and 96 h after in ... | 2007 | 18078609 |
| mycobiota of the date palm phylloplane: description and interactions. | we have analysed the mycobiota of date palm (phoenix dactylifera, l.) leaves using several techniques. profusely sporulating fungi (penicillium spp. and cladosporium spp.) developed when plating leaf fragments and leaf washings. fusarium oxysporum, was particularly abundant in leaves infested with the red scale insect phoenicococcus marlatti cockerell, 1899, but an undescribed lecanicillium cf. psalliotae was also found. dual and overlay cultures showed interactions between palm pathogens, entom ... | 2007 | 18095764 |
| implication of a regulator of g protein signalling (bbrgs1) in conidiation and conidial thermotolerance of the insect pathogenic fungus beauveria bassiana. | beauveria bassiana is an insect pathogenic fungus widely used as a biocontrol agent to infect and control insect pests. the conidium initiates pathogenesis and mediates disease transmission, however, little is known about genetic elements that control conidiation. here, the cloning and characterization of a regulatory g protein-signalling (rgs) gene bbrgs1 from b. bassiana is reported. phylogenetic analysis showed that bbrgs1 was orthologous to the rgs protein, flba from aspergillus nidulans. th ... | 2008 | 18201190 |
| selection of entomopathogenic fungi for aphid control. | twelve strains of entomopathogenic fungi such as lecanicillium lecanii, paecilomyces farinosus, beauveria bassiana, metarhizium anisopliae, cordyceps scarabaeicola, and nomuraea rileyi were screened for aphid control. at 25 degrees c and 75% relative humidity (rh), among tested entomopathogenic fungi, l. lecanii 41185 showed the highest virulent pathogenicity for both myzus persicae and aphis gossypii, and their control values were both nearly 100% 5 and 2 d after treatment, respectively. moreov ... | 2007 | 18215637 |
| protected by fumigants: beetle perfumes in antimicrobial defense. | beetles share with other eukaryotes an innate immune system that mediates endogenous defense against pathogens. in addition, larvae of some taxa produce fluid exocrine secretions that contain antimicrobial compounds. in this paper, we provide evidence that larvae of the brassy willow leaf beetle phratora vitellinae constitutively release volatile glandular secretions that combat pathogens in their microenvironment. we identified salicylaldehyde as the major component of their enveloping perfume ... | 2008 | 18236110 |
| authentic heterologous expression of the tenellin iterative polyketide synthase nonribosomal peptide synthetase requires coexpression with an enoyl reductase. | the tens gene encoding tenellin synthetase (tens), a 4239-residue polyketide synthase nonribosomal-peptide synthetase (pks-nrps) from beauveria bassiana, was expressed in aspergillus oryzae m-2-3. this led to the production of three new compounds, identified as acyl tetramic acids, and numerous minor metabolites. consideration of the structures of these compounds indicates that the putative c-terminal thiolester reductase (r) domain does not act as a reductase, but appears to act as a dieckmann ... | 2008 | 18266306 |
| addition of exogenous carbon and nitrogen sources to aphid exuviae modulates synthesis of proteases and chitinase by germinating conidia of beauveria bassiana. | secretion of catabolic extracellular enzymes (ece) is the hallmark of the infection of insects through the cuticle by entomopathogenic fungi (epf). in this paper, we show that germinating conidia of beauveria bassiana (bb) regulate the synthesis of ece through a multiple control mode during the initial stages of germination. we tested bb conidial growth on aphid exuviae with or without supplementation of additional carbon and/or nitrogen (c/n) compounds. to understand the interrelation between c ... | 2008 | 18286267 |
| molecular characterization of isolates of beauveria bassiana obtained from overwintering and summer populations of sunn pest (eurygaster integriceps). | to examine whether isolates of the entomopathogenic fungus beauveria bassiana are more closely associated to their summer hosts compared with overwintering hosts, with recently developed molecular tools based on mitochondrial regions. | 2008 | 18290810 |
| investigations into the taxonomy of the mushroom pathogen verticillium fungicola and its relatives based on sequence analysis of nitrate reductase and its regions. | the full sequence of the nitrate reductase gene was obtained from a type isolate of verticilliumfungicola var. fungicola and used for phylogenetic analysis against other ascomycete fungi. sequencing obtained 2749 bp of coding region, 668 bp of 5' flanking sequence and 731 bp of 3' flanking sequence. in silico analysis indicated that the coding region contains a single intron and translates into an 893 amino acid protein, with blast analysis identifying five conserved nitrate reductase domains wi ... | 2007 | 18297878 |
| effect of some biorational insecticides on spodoptera eridania in organic cabbage. | to reduce pest attack, several biorational products are allowed for use on organic vegetables in brazil. this study investigated eight biorational products applied singly or in combination against spodoptera eridania cramer in field plots of cabbage intercropped with coriander. the treatments were applied once a week over a 5 week period, beginning 34 days after transplanting. the evaluations consisted of counting the larvae of s. eridania on the day before and 7 and 21 days after spraying. the ... | 2008 | 18300205 |
| selection for resistance to a fungal pathogen in drosophila melanogaster. | an artificial selection experiment designed to explore the evolution of resistance to a fungal pathogen, beauveria bassiana, in drosophila melanogaster is reported here. the experiment was designed to test whether there is sufficient additive genetic variation in this trait for increased resistance to evolve, and, if so, whether there are correlated responses that might represent a cost to defence. after 15 generations of selection, flies from selected lines did not have higher overall fitness a ... | 2008 | 18301441 |
| comparison of application methods for suppressing the pecan weevil (coleoptera: curculionidae) with beauveria bassiana under field conditions. | the pecan weevil, curculio caryae (horn), is a key pest of pecans. the entomopathogenic fungus beauveria bassiana (balsamo) vuillemin is pathogenic to c. caryae. one approach to managing c. caryae may be application of b. bassiana directed toward adult weevils as they emerge from the soil to attack nuts in the tree canopy. our objective was to compare different application methods for suppression of c. caryae adults. treatments included direct application of b. bassiana (gha strain) to soil unde ... | 2008 | 18348807 |
| microbial metabolism of 1-aminoanthracene by beauveria bassiana. | the carcinogen and mutagen, 1-aminoanthracene, was efficiently metabolized by the fungal strain beauveria bassiana atcc 7159 to yield three new metabolites identified as 1-acetamido-5-[(4'-o-methyl-beta-d-glucopyranosyl)oxy]anthracene, 1-acetamido-8-[(4'-o-methyl-beta-d-glucopyranosyl)oxy]anthraquinone, and 1-acetamido-6-[(4'-o-methyl-beta-d-glucopyranosyl)oxy]anthraquinone, together with 1-acetamidoanthracene and 1-acetamidoanthraquinone. formation of these metabolites suggests that the metabol ... | 2008 | 18378148 |
| microbial metabolism part 9. structure and antioxidant significance of the metabolites of 5,7-dihydroxyflavone (chrysin), and 5- and 6-hydroxyflavones. | 5,7-dihydroxyflavone (chrysin) (1) when fermented with fungal cultures, aspergillus alliaceous (atcc 10060), beauveria bassiana (atcc 13144) and absidia glauco (atcc 22752) gave mainly 4'-hydroxychrysin (4), chrysin 7-o-beta-d-4-o-methylglucopyranoside (5) and chrysin 7-sulfate (6), respectively. mucore ramannianus (atcc 9628), however, transformed chrysin into six metabolites: 4'-hydroxy-3'-methoxychrysin (chrysoeriol) (7), 4'-hydroxychrysin (apigenin) (4) 3',4'-dihydroxychrysin (luteolin) (8), ... | 2008 | 18379084 |
| assessment of the suitability of tinopal as an enhancing adjuvant in formulations of the insect pathogenic fungus beauveria bassiana (bals.) vuillemin. | biopesticides based on beauveria bassiana (bals.) vuillemin hold great promise for the management of a wide range of insect pests. the conidia in the biopesticide formulation require an adjuvant to protect them from photoinactivation by sunlight. the suitability of tinopal, an optical brightener used as sunscreen for baculovirus formulations, for use with b. bassiana was assessed. the aim was to study the effect of tinopal on the growth and photoprotection of b. bassiana, and its effect on the s ... | 2008 | 18383501 |
| whey for mass production of beauveria bassiana and metarhizium anisopliae. | spore production of beauveria bassiana and metarhizium anisopliae was studied in a novel whey-based culture media. spore yield and viability were determined for two b. bassiana (gha-726 and ca-603) and two m. anisopliae (ca-1 and imi 330189) isolates following production in three whey-based systems: solid, liquid, and a diphasic production system. our study indicated that whey permeate can be used effectively for production of spores of entomopathogenic fungi. however, spore yield and viability ... | 2008 | 18396025 |
| pathogenicity of entomopathogenic fungi on hibernating pupae of cameraria ohridella deschka & dimic 1986 (lepidoptera, gracillariidae). part 1: pathogenicity against the naked pupa. | strains from paecilomyces fumosoroseus, lecanicillium muscarium, metarhizium anisopliae and beauveria bassiana were examined in standardized biotest to control the horse-chestnut leaf miner (cameraria ohridella) in her pupal stage in winter. the fungi were pathogenic against the hibernating pupae of cameraria ohridella at dose of 1.9 x 10(7) conidia/ml. they were aggressive, led to infection, death and mouldiness of naked pupae. even at low temperature of 5 degrees c and 12 degrees c. l. muscari ... | 2007 | 18399468 |
| pathogenicity of entomopathogenic fungi on hibernating pupae of cameraria ohridella deschka & dimic 1986 (lepidoptera, gracillariidae). part 2: efficacy of entomopathogenic fungi against pupa in her pupal cell. | the naked pupae of chestnut leafminer cameraria ohridella are susceptible for entomopathogenic fungi paecilomyces fumosoroseus strain p6; lecanicillium muscarium strain v24; metarhizium anisopliae strain m72 and beauveria bassiana strain b412. their activity at low temperature is advantageous especially for the use in the winter season. (richter et al., 2007a). therefore the possibility of infection should be examined in biotest for pupae in their pupal cells on leaf of chestnut at conditions li ... | 2007 | 18399469 |
| perspectives for the biological control of cameraria ohridella. | the horse chestnut leaf-miner, cameraria ohridella deschka et dimic (lepidoptera, gracillariidae) is a serious invasive pest of aesculus hippocastanum in europe. the larvae of this species feed on leaf parenchyma and can reduce the tree growth. we studied the impact of parasitoids on c. ohridella in the czech republic and also searched for entomopathogenic fungi associated with this pest. the results showed that the rate of parasitism varied between 5% and 15% in most cases. the most parasitized ... | 2007 | 18399483 |
| interaction effects between beauveria bassiana and imidacloprid against thrips tabaci (thysanoptera: thripidae). | the onion thrips, thrips tabaci lindeman is a major pest of many horticultural crops in many parts of the world. the entomopathogenic fungus beauveria bassiana (balsamo) vuillemin and imidacloprid are used for the control of the onion thrips. the potential interactions between the fungus and the chemical against the onion thrips adults were evaluated in a laboratory and two greenhouse bioassays. the laboratory bioassay was a leaf dip bioassay for a mixture of b. bassiana and imidacloprid. the fi ... | 2007 | 18399487 |
| an improved method for beauveria bassiana transformation using phosphinothricin acetyltransferase and green fluorescent protein fusion gene as a selectable and visible marker. | an improved transformation method for the biocontrol agent, beauveria bassiana, was developed. for convenience of transformation selection and detection, the coding regions of the genes for phosphinothricin acetyltransferase and green fluorescent protein were fused and an expression vector, pbft, carrying this fusion was constructed. under optimum conditions, over 60 transformants microg(-1) plasmid dna were obtained. b. bassiana conidia frozen 1 month at -80 degrees c were fully competent for t ... | 2008 | 18414799 |
| application timing and efficacy of alternatives for the insecticidal control of tipula paludosa meigen (diptera: tipulidae), a new invasive pest of turf in the northeastern united states. | two invasive crane flies, tipula paludosa meigen and tipula oleracea l. (diptera: tipulidae), were detected in new york state for the first time in 2004. both are damaging pests of turfgrass and other horticultural systems in north america where establishment has already been documented. to develop management recommendations for the northeast and define opportunities for preventive (autumn) and curative (spring) control, four insecticide trials targeting t. paludosa larvae were conducted over a ... | 2008 | 18416446 |
| cloning and expression of a gene encoding gallerimycin, a cysteine-rich antifungal peptide, from eri-silkworm, samia cynthia ricini. | a cdna clone encoding gallerimycin was isolated from larval fat body of immunized samia cynthia ricini and named as scr-gallerimycin. in naive larvae, no gene expression was detected, but strongly induced in fat body and hemocytes following immune challenge with bacteria or entomopathogenic fungus beauveria bassiana. strong expression of the gene was also induced by injection of peptidoglycan and zymosan, but very weakly by non-pathogenic fungus aspergillus oryzae. analysis of the sequence upstr ... | 2008 | 18436462 |
| beauveria bassiana: endophytic colonization and plant disease control. | seed application of beauveria bassiana 11-98 resulted in endophytic colonization of tomato and cotton seedlings and protection against plant pathogenic rhizoctonia solani and pythium myriotylum. both pathogens cause damping off of seedlings and root rot of older plants. the degree of disease control achieved depended upon the population density of b. bassiana conidia on seed. using standard plating techniques onto selective medium, endophytic 11-98 was recovered from surface-sterilized roots, st ... | 2008 | 18442830 |
| characterization of a highly active promoter, pbbgpd, in beauveria bassiana. | the promoter of glyceraldehyde-3-phosphate dehydrogenase (gpd) gene from aspergillus nidulans (pgpda) is widely used to direct expression of target genes constitutively in fungi. however, in some species, a heterogeneous promoter is found to be of low efficiency. to obtain a high-efficiency promoter for transformation of beauveria bassiana, an entomopathogenic fungus widely used as an mycoinsecticide, a glyceraldehyde-3-phosphate dehydrogenase gene (bbgpd) promoter, was cloned and characterized. ... | 2008 | 18443858 |
| identification of novel genes associated with conidiation in beauveria bassiana with suppression subtractive hybridization. | the conidiation of the entomopathogenic fungus beauveria bassiana (hyphomycete) is a complex process that involves the stage- and cell-type-specific expression of hundreds of genes. the suppression subtractive hybridization method was used to target genes involved in conidiation. seventeen genes were cloned that potentially were involved in conidia formation. six of them demonstrated differential expression between conidial and vegetative cultures. sequence analysis showed three cdna fragments h ... | 2008 | 18488350 |
| impact of two treatments of a formulation of beauveria bassiana (deuteromycota: hyphomycetes) conidia on varroa mites (acari: varroidae) and on honeybee (hymenoptera: apidae) colony health. | bee colonies in southern france were treated with conidia (asexual spores) from two strains of beauveria bassiana, an entomopathogenic fungus. one strain was commercial (gha) and the other had been isolated from varroa mites in the region (bb05002). objectives were to evaluate treatment effect on colony weight, adult bee mass, capped brood, and on varroa fall onto sticky boards. treatments included conidia formulated with either carnauba or candelilla wax powder, candelilla wax powder alone, or ... | 2008 | 18506583 |
| [effects of metarhizium anisopliae (metsch.) sorok. and beauveria bassiana (bals.) vuill. on the predatory stinkbug podisus nigrispinus (dallas) (hemiptera: pentatomidae)]. | entomopathogenic fungi and the predatory stinkbug podisus nigrispinus (dallas) can target simultaneously the same or different pests in agroecosystems. topical contact during fungal dispersion or spraying, walking on plant surfaces and ingestion of contaminated prey are some of possible ways of interactions between fungi and the predatory stinkbug. the impact of three isolates of metarhizium anisopliae (metsch.) sorok. (866, 1022 and 1189) and beauveria bassiana (bals.) vuill. (604, 634 and 561) ... | 2006 | 18575695 |
| sprays of emulsifiable beauveria bassiana formulation are ovicidal towards tetranychus urticae (acari: tetranychidae) at various regimes of temperature and humidity. | aerial conidia of beauveria bassiana in an emulsifiable formulation germinated by >95% after 24 h exposure to the regimes of 20, 25 and 30 degrees c with 51%, 74% and 95% rh. ovicidal activities of the formulation towards two-spotted spider mite, tetranychus urticae, were assayed at the concentrations of 0, 18, 160 and 693 conidia mm(-2) sprayed separately onto fava bean leaves including 39 (25-76) eggs per capita. all the sprayed eggs on the leaves were directly exposed to the different regimes ... | 2008 | 18584129 |
| increased pathogenicity against coffee berry borer, hypothenemus hampei (coleoptera: curculionidae) by metarhizium anisopliae expressing the scorpion toxin (aait) gene. | coffee berry borer (cbb) is the worlds most devastating coffee pest causing an estimated us$500 million worth of losses annually through damage and control costs. beauveria bassiana and metarhizium anisopliae have been employed to control this pest but their low virulence (slow kill and large inoculums) is an important factor constraining their use. m. anisopliae (aait-ma549) has been modified to express the scorpion toxin (aait) in insect hemolymph and this greatly increased pathogenicity again ... | 2008 | 18597773 |
| up-regulation of lysozyme gene expression during metamorphosis and immune challenge of the cotton bollworm, helicoverpa armigera. | lysozymes act as crucial bacteriolytic enzymes in insect immune system by hydrolyzing the beta (1-->4) bonds between n-acetylglucosamine and n-acetylmuramic acid in the peptidoglycan of prokaryotic cell walls. we have isolated and characterized a helicoverpa armigera cdna encoding an insect lysozyme named halyz. we amplified a fragment by pcr, using degenerate primers derived from the conservative amino acid sequences for performing 5' and 3' race. the full-length cdna was 661 base pairs. the th ... | 2009 | 18615707 |
| effect of temperature on virulence of beauveria bassiana and metarhizium anisopliae isolates to tetranychus evansi. | the virulence of three isolates of beauveria bassiana (bals.) vuill. and 23 isolates of metarhizium anisopliae (metschnik.) sorok. (ascomycota: hypocreales) against the tomato spider mite, tetranychus evansi baker and pritchard (acari: tetranychidae), was assessed in the laboratory. the effect of temperature on germination, radial growth and virulence of selected isolates (two isolates of b. bassiana and nine of m. anisopliae) on t. evansi was also investigated in the laboratory. all the fungal ... | 2008 | 18648993 |
| effects of bacillus thuringiensis toxin cry1ac and beauveria bassiana on asiatic corn borer (lepidoptera: crambidae). | in this study, interactions between cry1ac, a toxic crystal protein produced by bacillus thuringiensis (berliner), and beauveria bassiana on the mortality and survival of ostrinia furnacalis was evaluated in the laboratory. the results showed that cry1ac is toxic to o. furnacalis. not only were larval growth and development delayed, but pupation, pupal weight and adult emergency also decreased when larvae were fed on artificial diet containing purified cry1ac toxin. when third instars o. furnaca ... | 2008 | 18656480 |
| virulence of hypocreales fungi to pecan aphids (hemiptera: aphididae) in the laboratory. | there is need for efficacious biocontrol agents for aphids in commercial orchards. as a preliminary step to this end we determined the virulence of several hypocreales fungi to pecan aphids. in the first experiment we tested the virulence of isaria fumosorosea (arsef 3581) blastospores to three pecan aphids monellia caryella, melanocallis caryaefoliae, and monelliopsis pecanis under laboratory conditions. rates of 1x10(7) or 1x10(8) spores per ml were applied in 2 ml via a spray tower to 90 mm p ... | 2008 | 18675272 |
| microbial metabolism of biologically active secondary metabolites from nerium oleander l. | ursolic acid (1) and kaempferol (3) are two major constituents of the mediterranean plant nerium oleander l. microbial metabolism of (1) with aspergillus flavus (atcc 9170) resulted in the formation of 3-oxo-ursolic acid derivative, ursonic acid (2). on the other hand, cunninghamella blakesleeana (atcc 8688a) was able to convert (3) into kaempferol 3-o-beta-d-glucopyranoside (4) as well as the new natural product kaempferol 4'-sulfate (5). incubation of kaempferol with mucor ramannianus (atcc 96 ... | 2008 | 18758096 |
| gene expression of a novel defensin antimicrobial peptide in the silkworm, bombyx mori. | cdna encoding a novel defensin (bmdefensinb) was cloned from the fat body of the silkworm, bombyx mori, and gene expression was analyzed. bmdefensinb showed typical structural characteristics of invertebrate defensins. phylogenetic and bootstrap analyses indicated that it has no orthologs, whereas previously reported bmdefensina is the ortholog of spodoptera frugiperda (sf)spodoptericin. the bmdefensinb gene was expressed tissue-specifically in the fat body and was strongly activated by bacteria ... | 2008 | 18776686 |
| insecticidal evaluation of beauveria bassiana engineered to express a scorpion neurotoxin and a cuticle degrading protease. | to improve the insecticidal efficacy of the entomopathogen beauveria bassiana, the fungus was genetically modified with an insect-specific scorpion neurotoxin aait and an insect cuticle degrading protease pr1a from another insect pathogen (metarhizium anisopliae). the wild-type and the transformants were bioassayed against the larvae of masson's pine caterpillar dendrolimus punctatus and the wax moth galleria mellonella. in comparison to the wild-type strain, engineered isolates took fewer spore ... | 2008 | 18800183 |
| biosynthesis of the cyclooligomer depsipeptide beauvericin, a virulence factor of the entomopathogenic fungus beauveria bassiana. | beauvericin, a cyclohexadepsipeptide ionophore from the entomopathogen beauveria bassiana, shows antibiotic, antifungal, insecticidal, and cancer cell antiproliferative and antihaptotactic (cell motility inhibitory) activity in vitro. the bbbeas gene encoding the bbbeas nonribosomal peptide synthetase was isolated from b. bassiana and confirmed to be responsible for beauvericin biosynthesis by targeted disruption. bbbeas utilizes d-2-hydroxyisovalerate (d-hiv) and l-phenylalanine (phe) for the i ... | 2008 | 18804027 |
| glycosylation of xanthohumol by fungi. | a screening test on 29 microorganisms for transformation of xanthohumol led to the selection of twelve fungal strains. one of them, beauveria bassiana am278, converted xanthohumol into a glucosylated derivative. this product was identified as xanthohumol 4'-o-beta-d-4'''-methoxyglucopyranoside. | 2008 | 18811001 |
| entomopathogens (beauveria bassiana and steinernema carpocapsae) for biological control of bark-feeding moth indarbela dea on field-infested litchi trees. | the bark-feeding moth indarbela dea (swinhoe) is one of the major pests of litchi (litchi chinensis sonn.) in asia. effects on larval mortality and web production were tested by repeated, single and combined tree trunk applications of the entomopathogenic fungus beauveria bassiana (bals.) vuill. and the entomopathogenic nematode steinernema carpocapsae (weiser). two separate application trials were conducted in different seasons on young and older larvae respectively. | 2009 | 18823078 |
| [influence of different drying temperatures for solid substrate after fermentation on conidia characteristics of the entomopathogenic fungus beauveria bassiana]. | this study was to evaluate the effect of drying temperature for solid substrate after fermentation on conidia characteristics of entomopathogenic fungus beauveria bassiana. | 2008 | 18837365 |
| physiological implication of intracellular trehalose and mannitol changes in response of entomopathogenic fungus beauveria bassiana to thermal stress. | to explore possible role of intracellular trehalose accumulation in fungal tolerance to summer-like thermal stress, 3-day colonies of beauveria bassiana grown on a glucose-free medium at 25 degrees c were separately exposed to 35, 37.5 and 40 degrees c for 1-18 h, respectively. trehalose accumulation in stressed mycelia increased from initial 4.2 to 88.3, 74.7 and 65.5 mg g(-1) biomass after 6-h stress at 35, 37.5 and 40 degrees c, respectively, while intracellular mannitol level generally decli ... | 2009 | 18850325 |
| molecular characterization of a subgroup ie intron with wide distribution in the large subunit rrna genes of dermatophyte fungi. | group i introns have the ability to catalyse their own excision (self-splice) from pre-rna, and are found in a wide range of eukaryotic organisms. in fungal nuclear genomes, they have been identified in the small subunit (ssu) and large subunit (lsu) of the ribosomal rna gene. sequencing of the 3' region of the lsu rrna gene of the dermatophyte trichophyton interdigitale revealed a 393 bp group i intron, tin.2563, containing the four characteristic conserved motifs (p,q,r and s) essential for se ... | 2009 | 18949625 |
| pathogenicity of endophytic entomopathogenic fungi to ornithodoros erraticus and ornithodoros moubata (acari: argasidae). | the argasid ticks o. erraticus and o. moubata are of great medical and veterinary importance because they are vectors of the african swine fever virus and several species of human relapsing fever borreliae. biocontrol of these ticks using entomopathogenic fungi has not been previously reported. we examined the pathogenicity to different developmental stages of these two argasids of six strains of the fungal species beauveria bassiana (strains bb1764 and bb2157), lecanicillium lecanii (strains ll ... | 2008 | 18976863 |
| influence of temperature on virulence of fungal isolates of metarhizium anisopliae and beauveria bassiana to the two-spotted spider mite tetranychus urticae. | twenty-three isolates of metarhizium anisopliae (metschnikoff) sokorin and three isolates of beauveria bassiana (balsamo) vuillemin (ascomycota: hypocreales: clavicipitaceae) were assessed for their virulence against the two-spotted spider mite, tetranychus urticae koch (acari: tetranychidae). based on the screening results, nine isolates of m. anisopliae and two isolates of b. bassiana were tested for their virulence against young adult (1- to 2-day-old) female t. urticae at constant temperatur ... | 2009 | 18987988 |
| relationships among activities of extracellular enzyme production and virulence against helicoverpa armigera in beauveria bassiana. | extracellular enzymes produced by beauveria bassiana, are believed to play a key role in cuticle hydrolysis. enzyme production and pathogenicity has been found to be positively correlated. twenty-eight isolates of b. bassiana, collected from different geographical regions and host ranges were characterized by in vitro extracellular enzyme production and sds-page techniques for discerning biochemical basis for virulence among the different isolates. in vitro analysis of extracellular enzymes like ... | 2009 | 19025880 |
| effects of sheep fleece washings on the germination and growth of beauveria bassiana. | the development of biopesticides against ectoparasites must take into account the effect that an animal host's secretions and host associated micro-organisms may have on the viability of the applied agent. in this study, the effects of secretions washed from the pelt of sheep on the viability and growth of the fungal pathogen beauveria bassiana, were assessed. the fungal isolate had been obtained from the parasitic sheep scab mite psoroptes ovis. it was added to 0.05% tween 80 in which sheep fle ... | 2009 | 19041656 |
| cold stress increases resistance to fungal infection throughout life in drosophila melanogaster. | flies were subjected to one of three mild stresses known to have positive effects on longevity (heat, hypergravity, cold), prior to an infection with the entomopathogenic fungus beauveria bassiana. flies subjected to cold survived longer to infection, while the other mild stresses had no positive effect. these positive effects of a cold stress on resistance to infection were observed mainly in males and throughout life, i.e., a long time after the cold stress was applied. it was confirmed that c ... | 2009 | 19067222 |
| late stage oxidations during the biosynthesis of the 2-pyridone tenellin in the entomopathogenic fungus beauveria bassiana. | late stage oxidations during the biosynthesis of the 2-pyridone tenellin in the insect pathogenic fungus beauveria bassiana were investigated by a combination of gene knockout, antisense rna, and gene coexpression studies. open reading frames (orf) 3 and 4 of the tenellin biosynthetic gene cluster were previously shown to encode a trans-acting enoyl reductase and a hybrid polyketide synthase nonribosomal peptide synthetase (pks-nrps), respectively, which together synthesize the acyltetramic acid ... | 2008 | 19067514 |
| combinatorial mutasynthesis of scrambled beauvericins, cyclooligomer depsipeptide cell migration inhibitors from beauveria bassiana. | fungal cyclooligomer depsipeptides such as beauvericin, bassianolide, and enniatins display antibiotic, antifungal, insecticidal, broad-spectrum cancer cell antiproliferative, and cell migration inhibitory activities. we have identified a gene encoding a novel enzyme, ketoisovalerate reductase (kivr), which is the sole provider of d-hydroxyisovalerate (d-hiv), a common precursor for cyclooligomer depsipeptide biosynthesis in beauveria bassiana. kivr and related hypothetical oxidoreductases encod ... | 2009 | 19105175 |
| fungal formulations to control rhipicephalus sanguineus engorged females. | entomopathogenic fungi are promising alternatives to chemical acaricides in controlling tick populations; however, biological formulations are required to improve conidial performance of these pathogens in the field. this study evaluated the effect of in vitro treatment of rhipicephalus sanguineus with conidial formulations of beauveria bassiana (bb) or metarhizium anisopliae (ma). conidia were formulated (at 10(8) conidia ml(-1)) in (1) emulsifiable concentrate (ec), (2) polymerized cellulose g ... | 2008 | 19120220 |
| virulence of metarhizium anisopliae and beauveria bassiana to ctenocephalides felis felis. | ctenocephalides felis felis is a flea species that parasitizes dogs and cats. the current study verified the in vitro virulence of the entomopathogenic fungi metarhizium anisopliae and beauveria bassiana to eggs and adults of c. felis felis. the fungal isolates were cultivated on rice grains in plastic bags. conidial suspensions were then prepared by immersing the grains in tween 80 aqueous solution (0.01%). eggs and adults of c. felis felis were obtained from a laboratory colony. experiments we ... | 2008 | 19120257 |
| the size and ratio of homologous sequence to non-homologous sequence in gene disruption cassette influences the gene targeting efficiency in beauveria bassiana. | targeted gene replacement via homologous recombination (hr) is a conventional approach for the analysis of gene function. however, this event is rare in beauveria bassiana, which hampers efficient functional analysis in this widely used entomopathogenic fungus. to improve homologous recombination frequency in b. bassiana, we investigated the effect of the ratio of homologous sequence to non-homologous sequence (hs/nhs) in gene disruption cassette upon the hr frequency by two gene loci bbntl and ... | 2009 | 19148636 |
| effect of fungal infection on reproductive potential and survival time of tetranychus urticae (acari: tetranychidae). | the effect of fungal infection on the reproductive potential of two-spotted spider mite, tetranychus urticae, was evaluated as part of the full biocontrol potential of three entomopathogenic fungi by modeling of fecundity probability. female mites (<or=2-day-old) on leaves were exposed to the sprays of beauveria bassiana, paecilomyces fumosoroseus and metarhizium anisopliae at the concentrations of 1.13 x 10(3), 1.55 x 10(3) and 0.95 x 10(3) deposited conidia mm(-2) and then individually reared ... | 2009 | 19153812 |
| effects of temperature on metal tolerance and the accumulation of zn and pb by metal-tolerant fungi isolated from urban runoff treatment wetlands. | to investigate the ability of two fungi to accumulate zn and pb, the effect of temperature on their metal tolerance and possible mechanisms involved in metal accumulation. | 2009 | 19187150 |
| community composition, host range and genetic structure of the fungal entomopathogen beauveria in adjoining agricultural and seminatural habitats. | although intensively investigated for biological control of insect pests, little is known about the ecology of the fungal entomopathogenic genus beauveria in natural or agricultural habitats. in this study, we used molecular phylogenetic and genotypic information to infer species diversity, reproductive potential and genetic structure of beauveria occurring within a single arable field and bordering hedgerow in denmark. isolates were sampled from cultivated field and hedgerow soils, from insects ... | 2009 | 19226319 |
| eco-friendly management of hadda beetle using beauveria bassiana in brinjal. | brinjal (solanum melongena l.) is one among the vegetables grown in jaffna peninsula of sri lanka. it has been extensively cultivated throughout the country due to its demand among the consumers. the productivity of brinjal depends on the intensity of the attack of pests throughout the year. among the pests hadda beetle, epilachna vigintioctopunctata (coleoptera: coccinellidae) emerged as the key pest and caused serious damage on the foliage. this damage affected the flowering and fruiting abili ... | 2008 | 19226800 |
| humoral immune response of galleria mellonella larvae after infection by beauveria bassiana under optimal and heat-shock conditions. | natural infection of galleria mellonella larvae with the entomopathogenic fungus beauveria bassiana led to antifungal, but not antibacterial host response. this was manifested by induction of gallerimycin and galiomicin gene expression and, consequently, the appearance of antifungal activity in the hemolymph of the infected larvae. the activity of lysozyme increased at the beginning of infection and dropped while infection progressed. exposure of the naturally infected animals to 43 degrees c fo ... | 2009 | 19232408 |
| mmps regulate both development and immunity in the tribolium model insect. | matrix metalloproteinases (mmps) are evolutionarily conserved and multifunctional effector molecules in development and homeostasis. in spite of previous, intensive investigation in vitro and in cell culture, their pleiotrophic functions in vivo are still not well understood. | 2009 | 19270735 |
| pathogenicity of fungi to eggs of heterodera glycines. | twenty-one isolates of 18 fungal species were tested on water agar for their pathogenicity to eggs of heterodera glycines. an egg-parasitic index (epi) for each of these fungi was recorded on a scale from 0 to 10, and hatch of nematode eggs was determined after exposure to the fungi on water agar for 3 weeks at 24 c. the epi for verticillium chlamydosporium was 7.6, and the fungus reduced hatch 74%. pyrenochaeta terrestris and two sterile fungi also showed a high epi and reduced hatch 42-73%. ar ... | 1996 | 19277130 |
| biosynthesis of the cyclooligomer depsipeptide bassianolide, an insecticidal virulence factor of beauveria bassiana. | beauveria bassiana is a facultative entomopathogen with an extremely broad host range that is used as a commercial biopesticide for the control of insects of agricultural, veterinary and medical significance. b. bassiana produces bassianolide, a cyclooligomer depsipeptide secondary metabolite. we have cloned the bbbsls gene of b. bassiana encoding a nonribosomal peptide synthetase (nrps). targeted inactivation of the b. bassiana genomic copy of bbbsls abolished bassianolide production, but did n ... | 2009 | 19285149 |
| real-time quantitative pcr for analysis of candidate fungal biopesticides against malaria: technique validation and first applications. | recent research has indicated that fungal biopesticides could augment existing malaria vector control tools. here we present a set of methodologies to monitor the in vivo kinetics of entomopathogenic fungi in anopheles in the presence or absence of malaria parasites using quantitative real-time pcr. three qpcr assays were successfully developed for counting fungal genomes: "specific" assays capable of distinguishing two well characterized fungal entomopathogens beauveria bassiana isolate imi3915 ... | 2009 | 19320043 |
| selection of native isolates of beauveria bassiana (ascomycetes: clavicipitaceae) for the microbial control of rhipicephalus (boophilus) microplus (acari: ixodidae). | previously undiscovered isolates of beauveria bassiana (balsamo) vuillemin (ascomycetes: clavicipitaceae) able to control rhipicephalus (boophilus) microplus (canestrini) (acari: ixodidae) were obtained for the first time in argentina. the isolates were selected from three sources: 1) soil samples from the provinces of corrientes, formosa, and chaco, where ticks are endemic; 2) dead female ticks; and 3) the fungal collection from the entomopathogenic fungi laboratory of imyza-inta castelar. to s ... | 2009 | 19351079 |
| mitogen-activated protein kinase hog1 in the entomopathogenic fungus beauveria bassiana regulates environmental stress responses and virulence to insects. | beauveria bassiana is an economically important insect-pathogenic fungus which is widely used as a biocontrol agent to control a variety of insect pests. however, its insecticide efficacy in the field is often influenced by adverse environmental factors. thus, understanding the genetic regulatory processes involved in the response to environmental stress would facilitate engineering and production of a more efficient biocontrol agent. here, a mitogen-activated protein kinase (mapk)-encoding gene ... | 2009 | 19363067 |
| comparative tolerances of various beauveria bassiana isolates to uv-b irradiation with a description of a modeling method to assess lethal dose. | the tolerances of 20 beauveria bassiana isolates derived from host insects worldwide to uv-b irradiation were assessed quantitatively in multi-dose bioassays. conidial suspensions of the isolates smeared on glass slides were exposed to the gradient uv-b doses of 0.1-1.6 j cm(-2) (d), which generated from 0.75 to 10.17 min irradiation of weighted 312-nm wavelength at 2.0-2.61 mw cm(-2). irradiated conidia were then incubated for 24 h at 25 degrees c under saturated humidity. the ratio of germinat ... | 2009 | 19390989 |