Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| prospective study of human metapneumovirus detection in clinical samples by use of light diagnostics direct immunofluorescence reagent and real-time pcr. | a light diagnostics human metapneumovirus (hmpv) monoclonal antibody reagent was evaluated using cytospin-enhanced direct immunofluorescence (dfa), and the results were compared to those for taqman reverse transcription-pcr (rt-pcr). of the 202 samples tested, 48 were positive by rt-pcr, and 41 (85.4%) of these were positive by dfa (p = 0.0771). the commercial availability of an hmpv dfa reagent will be of significant benefit to clinical laboratories. | 2008 | 18184854 |
| human metapneumovirus pneumonia in adults: results of a prospective study. | we prospectively collected clinical, laboratory, and radiographic data regarding community-acquired human metapneumovirus (hmpv) infection in consecutive adults hospitalized with pneumonia. hmpv infection was diagnosed using highly accurate reverse-transcription-polymerase chain reaction analysis of nasopharyngeal samples. eight (4%) of 193 patients had hmpv rna present, all detected during influenza season. hmpv is an important cause of pneumonia in adults. | 2008 | 18194097 |
| molecular epidemiology of human metapneumovirus in ireland. | human metapneumovirus (hmpv) is a cause of respiratory illness ranging from wheezing to bronchiolitis and pneumonia in children. a quantitative real-time reverse-transcriptase polymerase chain reaction (rt-pcr) assay was developed for the detection of all four main genetic lineages of hmpv and employed to validate an indirect immunofluorescence (if) assay to detect hmpv positive specimens. the if assay detected 24 positives from a screen of 625 randomly selected pediatric respiratory specimens c ... | 2008 | 18205227 |
| human metapneumovirus inhibits ifn-alpha signaling through inhibition of stat1 phosphorylation. | the recently discovered human metapneumovirus (hmpv) is a major cause of lower and upper respiratory tract infections worldwide. acute viral infection initiates the interferon response that is critical in mediating viral clearance, viral host defense, and development of adaptive immunity. mouse models of infection suggest that hmpv can cause persistent lung infections, yet the mechanisms of evading host viral clearance are unknown. here we report that hmpv can subvert host type i interferon sign ... | 2008 | 18218993 |
| identification of human metapneumovirus-induced gene networks in airway epithelial cells by microarray analysis. | human metapneumovirus (hmpv) is a major cause of lower respiratory tract infections in infants, elderly and immunocompromised patients. little is known about the response to hmpv infection of airway epithelial cells, which play a pivotal role in initiating and shaping innate and adaptive immune responses. in this study, we analyzed the transcriptional profiles of airway epithelial cells infected with hmpv using high-density oligonucleotide microarrays. of the 47,400 transcripts and variants repr ... | 2008 | 18234263 |
| prevalence of human metapneumovirus in central illinois in patients thought to have respiratory viral infection. | studies suggest that by age 5 years nearly all people have been exposed to metapneumovirus. to determine its prevalence in central illinois, we tested respiratory secretions by direct immunofluorescence staining from december to march. metapneumovirus was detected in 11/391 specimens. the distribution of metapneumovirus was bimodal, with the split being between children aged </=4 years and adults. | 2008 | 18234862 |
| major histocompatibility complex class i cytotoxic t lymphocyte immunity to human metapneumovirus (hmpv) in individuals with previous hmpv infection and respiratory disease. | recently identified human metapneumovirus (hmpv) is an important respiratory pathogen in children and adults worldwide. little is known about cytotoxic t lymphocyte (ctl) responses that may control hmpv infection in humans. to address this, we evaluated major histocompatibility complex (mhc) class i t cell immunity in 7 patients with previous hmpv respiratory disease. ctl responses were evident in most patients and to most proteins of hmpv. individual patients had responses to at least 2 hmpv pr ... | 2008 | 18240952 |
| human metapneumovirus and human coronavirus nl63. | 2008 | 18245437 | |
| human metapneumovirus in children, singapore. | four hundred specimens were collected from pediatric patients hospitalized in singapore; 21 of these specimens tested positive for human metapneumovirus (hmpv), with the a2 genotype predominating. a 5% infection rate was estimated, suggesting that hmpv is a significant cause of morbidity among the pediatric population of singapore. | 2007 | 18252120 |
| respifinder: a new multiparameter test to differentially identify fifteen respiratory viruses. | broad-spectrum analysis for pathogens in patients with respiratory tract infections is becoming more relevant as the number of potential infectious agents is still increasing. here we describe the new multiparameter respifinder assay, which is based on the multiplex ligation-dependent probe amplification (mlpa) technology. this assay detects 15 respiratory viruses in one reaction. the mlpa reaction is preceded by a preamplification step which ensures the detection of both rna and dna viruses wit ... | 2008 | 18256230 |
| human metapneumovirus infection among children, bangladesh. | we confirmed circulation of human metapneumovirus (hmpv) among children with febrile and respiratory illness in an urban slum in dhaka, bangladesh, during active surveillance in 2001. hmpv was the most common single virus identified among febrile children and appears to contribute to the high rates of illness in this population. | 2007 | 18258022 |
| human metapneumovirus infections in children. | human metapneumovirus (hmpv) is an important cause of lower respiratory tract infections in hospitalized children, but the age-related incidence and effect of hmpv in unselected children in the community have not been evaluated. we studied a cohort of 1,338 children <13 years of age throughout 1 respiratory season in finland during 2000-2001. we examined children and obtained a nasal swab for viral detection at any sign of respiratory infection. hmpv was detected in 47 (3.5%) of the 1,338 childr ... | 2008 | 18258088 |
| clinical evaluation of nuclisens magnetic extraction and nuclisens analyte-specific reagents for real-time detection of human metapneumovirus in pediatric respiratory specimens. | in this study, we evaluated the nuclisens minimag (mm) and easymag (em) nucleic acid extraction platforms (biomérieux, durham, nc) in combination with the nuclisens easyq basic kit and analyte-specific reagents (asrs) (biomérieux) for the detection of human metapneumovirus (hmpv) in respiratory samples. total nucleic acids from pediatric clinical samples (n = 653) and an hmpv-specific inhibition control (h-ic) were coextracted using the mm and/or the em. nucleic acid sequence-based amplification ... | 2008 | 18272716 |
| prospective multicenter study of the viral etiology of bronchiolitis in the emergency department. | to determine the viral etiology of bronchiolitis and clinical characteristics of children age < 2 years presenting to the emergency department (ed) with bronchiolitis. | 2008 | 18275439 |
| [virology: the contribution of molecular biology in the microbiologic diagnosis of pediatric respiratory diseases]. | the conventionnal tools used for virological diagnosis include direct antigen detection by immunofluorescence (ifa) or an immunoenzymatic test (eia), and viral isolation technique (vit). in most cases, ifa and eia have a slightly lower sensitivity than vit but are also able to detect some vit-negative samples. results of several teams using rt-pcr technologies show that the molecular methods detect more positive cases than the conventional tools. work is under way to expand the number of viruses ... | 2007 | 18280911 |
| impact of human bocavirus on children and their families. | this study was planned to investigate the prevalence and clinical features of the illnesses associated with human bocavirus (hbov) in children with acute disease. we prospectively enrolled all subjects aged less than 15 years attending an emergency room in milan, italy, on wednesdays and sundays between 1 november 2004 and 31 march 2005 for any acute medical reason, excluding surgical diseases and trauma. nasopharyngeal swabs were collected at admission to detect hbov; influenza a and b viruses; ... | 2008 | 18287315 |
| two-year prospective study of single infections and co-infections by respiratory syncytial virus and viruses identified recently in infants with acute respiratory disease. | a prospective 2-year analysis including 322 infant patients with acute respiratory disease (ard) hospitalized in a pediatric department in northern italy was carried out to evaluate the role as respiratory pathogens or co-pathogens of recently identified viruses. the presence of respiratory syncitial virus (rsv), human metapneumoviruses (hmpvs), human bocaviruses (hbovs), and human coronaviruses (hcovs) was assayed by molecular detection and clinical symptoms evaluated. nasopharyngeal aspirates ... | 2008 | 18297694 |
| undiagnosed respiratory viruses in children. | an estimated 12 to 32 million upper respiratory infections occur in young children each year. in addition, 20% to 53% of infants will have > or = 1 episode of lower respiratory infection in the first year of life. the current methods of diagnosing respiratory viruses are limited in scope and sensitivity. polymerase chain reaction is a more sensitive method than antigen detection and is often used for newly discovered viruses. using polymerase chain reaction, we sought to diagnose adenoviruses, h ... | 2008 | 18310182 |
| prospective evaluation of rapid antigen tests for diagnosis of respiratory syncytial virus and human metapneumovirus infections. | respiratory syncytial virus (rsv) and human metapneumovirus (hmpv) are two important viral pathogens that cause respiratory tract infections in the pediatric population. the rapid detection of these agents allows the prompt isolation and treatment of infected patients. in the present prospective study, we evaluated the performances of four rapid antigen detection assays, including a rapid chromatographic immunoassay (cia) for rsv (directigen ez rsv; becton dickinson, sparks, md), a direct fluore ... | 2008 | 18337386 |
| [clinical and epidemiological characteristics of respiratory infections caused by human metapneumovirus in pediatric patients]. | human metapneumovirus (hmpv) was first identified in 2001 in respiratory samples from children and adults with acute respiratory tract infection. the aim of this prospective study was to determine the clinical and epidemiological characteristics of pediatric patients with an acute respiratory tract infection and exclusive isolation of hmpv in respiratory samples (december 2005-january 2007). | 2008 | 18341917 |
| specificity and functional interaction of the polymerase complex proteins of human and avian metapneumoviruses. | human metapneumovirus (hmpv) and avian metapneumovirus (ampv) have a similar genome organization and protein composition, but a different host range. ampv subgroup c (ampv-c) is more closely related to hmpv than other ampvs. to investigate the specificity and functional interaction of the polymerase complex proteins of human and avian metapneumoviruses, a minireplicon system was generated for ampv-c and used in combination with minireplicon systems for hmpv lineages a1 and b1. viral rna-like mol ... | 2008 | 18343839 |
| immune modulatory activity of ribavirin for serious human metapneumovirus disease: early i.v. therapy may improve outcomes in immunosuppressed sct recipients. | 2008 | 18347567 | |
| respiratory viruses in hiv-infected patients with suspected respiratory opportunistic infection. | to assess the incidence and epidemiological pattern of respiratory viruses in hiv-infected patients and to evaluate their potential clinical impact. | 2008 | 18356599 |
| genotype variability of human metapneumovirus, south korea. | human metapneumovirus (hmpv), a virus causing lower respiratory tract infections in children, is classified two major groups or genotypes of hmpv and recently existence of multiple lineages has been suggested. the purpose of this study was to examine the extent of genetic variation and circulation pattern of hmpv in korea. between january 2005 and april 2007, nasopharyngeal aspirates were collected from 1,214 children <16 years of age hospitalized with acute respiratory tract infection at sanggy ... | 2008 | 18360903 |
| role of human metapneumovirus, human coronavirus nl63 and human bocavirus in infants and young children with acute wheezing. | the role of the novel respiratory viruses, human metapneumovirus (hmpv), human coronavirus nl63 (hcov nl63) and human bocavirus (hbov), in wheezing illness in children has not been well studied, especially in africa. the aim of this study was to investigate the prevalence of hmpv, hcov nl63 and hbov in south african children with acute wheezing. a prospective study of consecutive children presenting with acute wheezing to a pediatric hospital from may 2004 to november 2005 was undertaken. a nasa ... | 2008 | 18360904 |
| comparison of human metapneumovirus genotypes from the province of bolzano in northern italy with strains from surrounding regions in italy and austria. | the epidemiology of the genetic sublineages of human metapneumovirus (hmpv) and their clinical relevance are not fully understood. we compared hmpv genotypes isolated in the province of bolzano in northern italy with strains from nearby italian and austrian regions by sequencing of np- and l-gene fragments. our results suggest that similar strains cycle through adjacent geographic areas, with the respective subtypes replacing each other on a seasonal basis. | 2008 | 18362411 |
| evaluation of a commercial direct fluorescent-antibody assay for human metapneumovirus in respiratory specimens. | the performance of a commercially manufactured direct fluorescent-antibody assay kit for human metapneumovirus was compared to that of reverse transcriptase pcr, the current "gold standard." the kit demonstrated a sensitivity of 95.2%, a specificity of 100%, and an accuracy of 98.9%. | 2008 | 18367577 |
| viral respiratory infections at the hajj: comparison between uk and saudi pilgrims. | a high incidence of respiratory infection, including influenza, has been reported at the hajj in mecca, saudi arabia. reported rates of influenza have been higher among uk than among domestic pilgrims, but this could be explained by methodological differences among studies. accordingly, the present study compared the frequencies of respiratory viruses among uk and saudi pilgrims using the same study design. pilgrims with upper respiratory tract symptoms were recruited from mecca and the neighbou ... | 2008 | 18373688 |
| human metapneumovirus infection in young children hospitalized with acute respiratory tract disease: virologic and clinical features. | human metapneumovirus (hmpv) is an emerging virus associated with acute respiratory tract infections (aris) in young children. | 2008 | 18382388 |
| the viral bronchiolites diagnosis in children by pcr multiplex. | the aim of the study was to determine the etiology of the viral bronchiolites in children by using direct immunofluorescence test and 3 rt-pcr multiplex (s.bellau-pujol) the study was performed on 122 nasal inspirations collected from 3 weeks-6 month old children hospitalizated in the pediatrics service of ch rouen. the results were that the majority (53%) of bronchiolites in children had like etiology rsv and a lot of these infections had double viral etiology (26% rsv+ rhinovirus; 2,7% rsv+hmp ... | 2006 | 18389721 |
| the prophylactic administration of a monoclonal antibody against human metapneumovirus attenuates viral disease and airways hyperresponsiveness in mice. | human metapneumovirus (hmpv) is one of the most frequent causes of respiratory tract infections in children. our objective was to assess the prophylactic benefit of a monoclonal antibody (mab) against the hmpv fusion protein in a murine model. | 2008 | 18389897 |
| emerging respiratory agents: new viruses for old diseases? | the recent advances in molecular technology have enabled the detection of several new viral agents in specimens collected from the human respiratory tract. human metapneumovirus was first described in 2001, and is a significant respiratory pathogen, particularly of children. following the identification of severe acute respiratory syndrome (sars) associated coronavirus, two other newly detected coronaviruses, nl63 and hku1, have been linked to respiratory disease in humans. however, identifying ... | 2008 | 18406664 |
| respiratory viruses in laryngeal croup of young children. | to determine the viral cause of laryngeal croup by use of highly sensitive methods, and including recently recognized viruses in the analysis. | 2008 | 18410770 |
| hospitalization rates for human metapneumovirus infection among 0- to 3-year-olds in gipuzkoa (basque country), spain. | numerous studies have been published on human metapneumovirus (hmpv) infection, but few have been population based. the main aim of this study was to estimate the incidence rate of hospitalization for community-acquired hmpv infection in infants and children aged <3 years. between july 2004 and june 2007, 796 episodes (742 patients) of community-acquired acute respiratory infection were hospitalized. hmpv was detected in 90 episodes (11.3%). fifty-nine episodes occurred in infants aged <1 year. ... | 2009 | 18419854 |
| human metapneumovirus infection in an immunocompetent adult presenting as mononucleosis-like illness. | a 37-year-old man was presented with incidental findings of neutropenia, atypical lymphocytosis, thrombocytopenia and deranged liver parenchymal enzymes. four days later, he developed fever, sore throat and cervical lymphadenopathy, compatible with mononucleosis-like illness (mli). polymerase chain reaction (pcr) and viral culture of the nasopharyngeal swab showed human metapneumovirus (hmpv). there was a >/=4-fold rise in igg against hmpv. this is the first case report illustrating the natural ... | 2008 | 18423876 |
| clinical impact of human metapneumovirus genotypes and genotype-specific seroprevalence in yamagata, japan. | the clinical impact of human metapneumovirus (hmpv) genotypes and the relation between the hmpv genotype in circulation and genotype-specific seroprevalence are yet to be clarified. we determined the genotypes of 93 hmpv strains that were isolated between 2004 and 2006 in yamagata, japan, and identified 35 genotype a2, 14 genotype b1, and 44 genotype b2 isolates. children infected with genotype a2 hmpv were significantly older than those infected with genotype b1 hmpv. diagnosis of laryngitis wa ... | 2008 | 18428137 |
| human metapneumovirus infection in immunocompromised child. | 2008 | 18439384 | |
| use of throat swab or saliva specimens for detection of respiratory viruses in children. | nasopharyngeal (np) specimens are commonly used for the detection of respiratory viruses, but throat and saliva specimens are easier to obtain. the objective of this study was to compare the viral yield of direct fluorescent antigen detection of np specimens and nucleic acid amplification tests (nat) of direct fluorescent antigen-negative np specimens with the viral yield of nat of throat swab and saliva specimens. | 2008 | 18444806 |
| the highest prevalence of human metapneumovirus in ahwaz children accompanied by acute respiratory infections. | the newly described human metapneumovirus (hmpv) has been recently discovered as an etiological agent of acute respiratory infections (arti) in infants and children. the aim of this study was to determine the prevalence of hmpv and its potential role as causative agent of arti in ahwaz children. | 2008 | 18445946 |
| detection and characterisation of human metapneumovirus from children with acute respiratory symptoms in north-west england, uk. | human metapneumovirus (hmpv) causes a spectrum of respiratory disease ranging from trivial coryzal symptoms to fatal pneumonia, with a predilection for the very young, the immune suppressed and the frail elderly. five distinct lineages of the virus genome have been described. | 2008 | 18455957 |
| [evaluation of seeplex rv detection kit for detecting rhinovirus, human metapneumovirus, and coronavirus]. | direct antigen test (dat) and culture are primary tests to diagnose infections by respiratory viruses, but are mainly available for the traditional viral pathogens such as respiratory syncytial virus (rsv), influenza virus, parainfluenza virus (piv), and adenovirus in clinical laboratories. the objective of this study was to evaluate a multiplex reverse transcriptase-pcr method using seeplex rv detection kit (seegene, korea) for the detection of rhinovirus, coronavirus, and human metapneumovirus ... | 2008 | 18458506 |
| acute febrile respiratory illness in the icu: reducing disease transmission. | acute febrile respiratory illness (fri) leading to respiratory failure is a common reason for admission to the icu. viral pneumonia constitutes a portion of these cases, and often the viral etiology goes undiagnosed. emerging viral infectious diseases such as severe acute respiratory syndrome and avian influenza present with acute fris progressing to respiratory failure and ards. therefore, early recognition of a viral cause of acute fri leading to ards becomes important for protection of health ... | 2008 | 18460521 |
| detection of genetic lineages of human metapneumovirus in croatia during the winter season 2005/2006. | human metapneumovirus (hmpv) is an important respiratory pathogen, especially among young children. the genetic characteristics of hmpv circulating in croatia have not been studied so far. the aim of this study was to determine the incidence of hmpv infection in hospitalized children with acute respiratory tract infection (arti) in the season 2005/2006 in croatia, as well as to perform the genotypic analysis of detected hmpv strains. from december 1 to march 31 nasopharyngeal secretions (npss) w ... | 2008 | 18461619 |
| association between high nasopharyngeal viral load and disease severity in children with human metapneumovirus infection. | previous studies have shown that viral genotype and viral load may play a significant role in the pathogenesis of viral infections. | 2008 | 18479963 |
| human rhinoviruses: the cold wars resume. | human rhinoviruses (hrvs) are the most common cause of viral illness worldwide but today, less than half the strains have been sequenced and only a handful examined structurally. this viral super-group, known for decades, has still to face the full force of a molecular biology onslaught. however, newly identified viruses (nivs) including human metapneumovirus and bocavirus and emergent viruses including sars-cov have already been exhaustively scrutinized. the clinical impact of most respiratory ... | 2008 | 18502684 |
| analysis of monthly isolation of respiratory viruses from children by cell culture using a microplate method: a two-year study from 2004 to 2005 in yamagata, japan. | although well over 200 viral agents have been implicated in acute respiratory infections (aris) among children, no system able to detect such a wide range of viruses has been established. between january 2004 and december 2005, a modified microplate method, including hef, hep-2, vero e6, mdck, rd-18s, and gmk cell lines (hhve6mrg plate), was adopted to isolate viruses. a total of 1,551 viruses were isolated, representing both outbreaks and sporadic cases, from 4,107 nasopharyngeal specimens, at ... | 2008 | 18503168 |
| respiratory failure associated with human metapneumovirus infection in an infant posthepatic transplant. | this is the first reported case of respiratory failure associated with human metapneumovirus (hmpv) infection in a liver transplant recipient or in a pediatric solid transplant recipient. a 9-month-old female developed respiratory distress 8 days following a liver transplant. hmpv was detected and she required intubation followed by extracorporeal membrane oxygenation for 26 days. immunosuppressive medications were stopped during the acute infection except for methylprednisolone as treatment for ... | 2008 | 18513273 |
| [human metapneumovirus]. | the human metapneumovirus (hmpv) is a new pneumovirinae related to the avian metapneumovirus type c. hmpv genome differs from human respiratory syncytial virus (rsv) genome by the gene order and the lack of nonstructural genes. two genetic sub-groups and four sub-types of hmpv are identified. hmpv infections evolve as regular winter outbreaks which have roughly the same size and overlaping rsv epidemics. among hospitalized children in caen, hmpv is detected in 9.7% of the cases after rsv (37%), ... | 2009 | 18515017 |
| frequency of human metapneumovirus infection in hematopoietic sct recipients during 3 consecutive years. | respiratory viruses can cause significant morbidity in immunocompromised hosts. human metapneumovirus (hmpv) has been increasingly associated with lower respiratory tract infection in hematopoietic sct (hsct) recipients, with mortality rates up to 50%. no data on the occurrence of hmpv infection in hsct recipients have been reported in the southern hemisphere. we conducted a retrospective study including 228 nasal wash samples from 153 hsct recipients with respiratory symptoms during 2001, 2002 ... | 2008 | 18516078 |
| human metapneumovirus glycoprotein g inhibits innate immune responses. | human metapneumovirus (hmpv) is a leading cause of acute respiratory tract infection in infants, as well as in the elderly and immunocompromised patients. no effective treatment or vaccine for hmpv is currently available. a recombinant hmpv lacking the g protein (rhmpv-delta g) was recently developed as a potential vaccine candidate and shown to be attenuated in the respiratory tract of a rodent model of infection. the mechanism of its attenuation, as well as the role of g protein in modulation ... | 2008 | 18516301 |
| deletion of human metapneumovirus m2-2 increases mutation frequency and attenuates growth in hamsters. | human metapneumovirus (hmpv) infection can cause acute lower respiratory tract illness in infants, the immunocompromised, and the elderly. currently there are no licensed preventative measures for hmpv infections. using a variant of hmpv/nl/1/00 that does not require trypsin supplementation for growth in tissue culture, we deleted the m2-2 gene and evaluated the replication of rhmpv/deltam2-2 virus in vitro and in vivo. | 2008 | 18519001 |
| the treatment of bronchiolitis. | bronchiolitis is the commonest reason for hospital admission in infancy and the most frequent cause of acute respiratory failure in children admitted to paediatric intensive care units in the uk and north america. the respiratory syncytial virus accounts for most cases of bronchiolitis, however, new virus isolation techniques have led to the discovery of previously unrecognised viruses, including the human metapneumovirus and bocavirus which also play a significant role. the main developments in ... | 2008 | 18539685 |
| stability of human metapneumovirus and human coronavirus nl63 on medical instruments and in the patient environment. | 2008 | 18541340 | |
| human metapneumovirus small hydrophobic protein inhibits nf-kappab transcriptional activity. | human metapneumovirus, a leading cause of respiratory tract infections in infants, encodes a small hydrophobic (sh) protein of unknown function. in this study, we showed that infection of airway epithelial cells or mice with recombinant human metapneumovirus lacking sh expression (rhmpv-deltash) enhanced secretion of proinflammatory mediators, including interleukin 6 (il-6) and il-8, encoded by two nf-kb-dependent genes, compared to infection with wild-type rhmpv. rhmpv-deltash infection resulte ... | 2008 | 18550666 |
| prevalence of human metapneumovirus among hospitalized children younger than 1 year in catalonia, spain. | human metapneumovirus was discovered recently respiratory virus implicated in both upper and lower respiratory tract infection. in children, the clinical symptoms of human metapneumovirus are similar to those produced by respiratory syncytial virus, ranging from mild to severe diseases such as bronchiolitis and pneumonia. the aim of the present study was to describe the prevalence of human metapneumovirus and other common respiratory viruses among admitted to hospital infants. from january 2006 ... | 2008 | 18551601 |
| long-term care facilities: a cornucopia of viral pathogens. | to determine the frequency and types of respiratory viruses circulating in boston long-term care facilities (ltcfs) during a 3-year period. | 2008 | 18557966 |
| role of respiratory pathogens in infants hospitalized for a first episode of wheezing and their impact on recurrences. | in order to evaluate the infectious agents associated with the first episode of severe acute wheezing in otherwise healthy infants and to define the role of each of them in recurrences, 85 patients in italy, aged <12 months, hospitalized because of a first acute episode of wheezing, were prospectively enrolled between 1 october 2005 and 31 march 2006. upon enrollment, nasopharyngeal swabs were collected for the real-time pcr detection of respiratory syncytial virus (rsv) types a and b, influenza ... | 2008 | 18558940 |
| generation of temperature-sensitive human metapneumovirus strains that provide protective immunity in hamsters. | human metapneumovirus (hmpv) causes acute respiratory tract illness primarily in young children, immunocompromised individuals and the elderly. vaccines would be desirable to prevent severe illnesses in these risk groups. here, we describe the generation and evaluation of cold-passage (cp) temperature-sensitive (ts) hmpv strains as vaccine candidates. repeated passage of hmpv at low temperatures in vero cells resulted in the accumulation of mutations in the viral genome. introduction of these mu ... | 2008 | 18559924 |
| t lymphocytes contribute to antiviral immunity and pathogenesis in experimental human metapneumovirus infection. | human metapneumovirus (hmpv), a member of the family paramyxoviridae, is a leading cause of lower respiratory tract infections in children, the elderly, and immunocompromised patients. virus- and host-specific mechanisms of pathogenesis and immune protection are not fully understood. by an intranasal inoculation model, we show that hmpv-infected balb/c mice developed clinical disease, including airway obstruction and hyperresponsiveness (ahr), along with histopathologic evidence of lung inflamma ... | 2008 | 18562525 |
| efficient multiplication of human metapneumovirus in vero cells expressing the transmembrane serine protease tmprss2. | human metapneumovirus (hmpv) is a major causative agent of severe bronchiolitis and pneumonia. its fusion (f) protein must be cleaved by host proteases to cause membrane fusion, a critical step for virus infection. by generating vero cells constitutively expressing the transmembrane serine protease tmprss2 and green fluorescent protein-expressing recombinant hmpv, we show that tmprss2, which is expressed in the human lung epithelium, cleaves the hmpv f protein efficiently and supports hmpv multi ... | 2008 | 18562527 |
| [analysis of human metapneumovirus genotype in hunan, china and its attachment protein g sequence character]. | to understand the genotypes of human metapneumovirus (hmpv) and the genetic character of hmpv attachment protein g sequence in hunan, china. | 2008 | 18574527 |
| human metapneumovirus: an emerging respiratory pathogen. | human metapneumovirus (hmpv) is an important emerging respiratory pathogen, previously unreported in the emergency medicine literature. it is primarily associated with the clinical syndromes of bronchiolitis and pneumonia. hmpv may predispose to bacterial pneumonia; coinfection with respiratory syncytial virus may lead to increased severity of clinical disease, and complications include asthma and chronic obstructive pulmonary disease exacerbations. given its high prevalence and potential clinic ... | 2010 | 18584996 |
| immunogenicity and efficacy of two candidate human metapneumovirus vaccines in cynomolgus macaques. | human metapneumovirus (hmpv) is an important cause of acute respiratory tract disease for which the development of vaccine candidates is warranted. we have previously described the generation of an iscom matrix-adjuvanted hmpv fusion protein subunit vaccine (fsol) and a live-attenuated vaccine (hmpvm11). here, we evaluate the immunogenicity and efficacy of these vaccines in cynomolgus macaques. immunization with fsol induced hmpv f-specific antibody responses, virus neutralizing antibody titers, ... | 2008 | 18585830 |
| low-ph-induced membrane fusion mediated by human metapneumovirus f protein is a rare, strain-dependent phenomenon. | membrane fusion promoted by human metapneumovirus (hmpv) fusion (f) protein was suggested to require low ph (r. m. schowalter, s. e. smith, and r. e. dutch, j. virol. 80:10931-10941, 2006). using prototype f proteins representing the four hmpv genetic lineages, we detected low-ph-dependent fusion only with some lineage a proteins and not with lineage b proteins. a glycine at position 294 was found responsible for the low-ph requirement in lineage a proteins. only 6% of all hmpv lineage a f seque ... | 2008 | 18596097 |
| detection of human metapneumovirus by direct antigen test and shell vial cultures using immunofluorescent antibody staining. | human metapneumovirus (hmpv) has emerged as an important etiologic agent of upper and lower respiratory tract infections, especially in young children. although rapid and simple diagnostic methods for hmpv are needed in clinical laboratories, routine diagnostic tests are not readily available. the purpose of this study was to evaluate a commercial anti-hmpv monoclonal antibody for a direct antigen test and a shell vial culture of hmpv. in the pilot study, 15 nasopharyngeal aspirates from 15 chil ... | 2008 | 18602421 |
| human metapneumovirus pneumonia in children: results of an italian study and mini-review. | human metapneumovirus (hmpv) is a newly identified paramyxovirus causing lower respiratory tract infections (lrti). current knowledge on hmpv is mainly based on retrospective studies performed in stored respiratory and serum samples. we found 15 previous prospective clinical studies on lrti (11 clinical and 4 epidemiological studies) that have been reviewed. our aims were to analyse the role of hmpv in community acquired pneumonia (cap) and the seroconversion rate to hmpv in a prospective study ... | 2008 | 18618373 |
| detection of four genetic subgroup-specific antibodies to human metapneumovirus attachment (g) protein in human serum. | human metapneumovirus (hmpv) strains are classified into two genetic groups, a and b, each of which is further divided in two genetic subgroups, a1, a2, b1 and b2. hmpv encodes two major surface glycoproteins, the fusion (f) and attachment (g) proteins, which may be immunogenic and protective antigens. although the amino acid sequences of hmpv f protein are highly conserved, those of the g protein are highly variable with low amino acid identity between the two groups. to address the antigenic v ... | 2008 | 18632969 |
| role of retinoic acid inducible gene-i in human metapneumovirus-induced cellular signalling. | human metapneumovirus (hmpv) is a recently discovered pathogen that causes a significant proportion of respiratory infections in young infants, the elderly and immunocompromised patients. very little is known regarding the cellular signalling elicited by this virus in airway epithelial cells, the target of hmpv infection. in this study, we investigated the role of the rna helicases retinoic acid inducible gene-i (rig-i) and melanoma differentiation-associated gene-5 (mda-5) as the main pattern r ... | 2008 | 18632970 |
| epidemiologic characteristics and seasonal distribution of human metapneumovirus infections in five epidemic seasons in stockholm, sweden, 2002-2006. | the presence of human metapneumovirus (hmpv) was analyzed retrospectively by reverse transcriptase-polymerase chain reaction (rt-pcr) in five epidemic seasons, in stockholm, 2002-2006. the occurrence of hmpv was compared with five common respiratory viruses; respiratory syncytial virus, influenza a virus, influenza b virus, parainfluenza virus and adenovirus. with a detection rate of 2.9% (n = 143/4,989) in nasopharyngeal samples over the whole period, hmpv was the fourth most common respiratory ... | 2008 | 18649344 |
| human metapneumovirus in lung transplant recipients and comparison to respiratory syncytial virus. | human metapneumovirus is a newly described virus isolated in 2001 from children with acute respiratory viral infection. it has subsequently been reported globally, although there are limited data in lung transplant recipients. | 2008 | 18658110 |
| respiratory viral infection in exacerbations of copd. | patients with copd have frequent exacerbations. the role of respiratory viral infection is just emerging. we wished to determine prospectively the incidence of viral infection in exacerbated and stable copd patients as well as smokers who do not have airways obstruction. | 2008 | 18672353 |
| seasonal invasive pneumococcal disease in children: role of preceding respiratory viral infection. | our objective was to demonstrate correlations between invasive pneumococcal disease in children and circulating respiratory viruses. | 2008 | 18676537 |
| human metapneumovirus reinfection among children in thailand determined by elisa using purified soluble fusion protein. | human metapneumovirus (hmpv) is a newly discovered paramyxovirus that causes acute respiratory illness. despite apparent near-universal exposure during early childhood, immunity is transient. | 2008 | 18680407 |
| viral infection in adults hospitalized with community-acquired pneumonia: prevalence, pathogens, and presentation. | the potential role of respiratory viruses in the natural history of community-acquired pneumonia (cap) in adults has not been well described since the advent of nucleic amplification tests (nats). | 2008 | 18689592 |
| viral diagnostics and antiviral therapy in hematopoietic stem cell transplantation. | viral infections are important causes of morbidity and mortality in hematopoietic stem cell transplant (hsct) recipients. some viruses, such as the respiratory and gastrointestinal viruses, are acquired from the healthcare or community in the midst of or after hsct. other viruses, such as the herpes-virus family, establish latency after resolution of primary infection but then may reactivate during the immunosuppression that occurs with hsct. due to the improved sensitivity and turn-around time ... | 2008 | 18691110 |
| informing the front line about common respiratory viral epidemics. | the nature of clinical medicine is to focus on individuals rather than the populations from which they originate. this orientation can be problematic in the context of acute healthcare delivery during routine winter outbreaks of viral respiratory disease where an individuals likelihood of viral infection depends on knowledge of local disease incidence. the level of interest in and perceived utility of community and regional infection data for front line clinicians providing acute care is unclear ... | 2007 | 18693841 |
| viruses in community-acquired pneumonia in children aged less than 3 years old: high rate of viral coinfection. | the occurrence of viral coinfections in childhood pneumonia has received little attention, probably because suitable detection methods have been lacking. between november 2004 and october 2006, the presence of 14 respiratory viruses in children aged less than 3 years old with community-acquired pneumonia were investigated using molecular or immunochromatographic techniques and/or viral culture. a total of 315 children (338 episodes) were included, and hospitalization was required in 178 episodes ... | 2008 | 18712820 |
| [human bocavirus-infection (hbov): an important cause of severe viral obstructive bronchitis in children]. | apart from established pathogens of lower respiratory tract infections, such as respiratory syncytial virus (rsv), an increasing number of additional agents has been identified in recent years. in 2005 the human bocavirus (hbov) has been isolated from respiratory tract samples and has been reported worldwide with frequencies ranging from 1.5 to 18.3% in respiratory samples from children with airway infections. | 2008 | 18716982 |
| optimization of plasmid-only rescue of highly attenuated and temperature-sensitive respiratory syncytial virus (rsv) vaccine candidates for human trials. | respiratory syncytial virus (rsv) is the most common cause of severe bronchiolitis in infants and young children in the u.s. no licensed rsv vaccines are currently available. established techniques for recovering rsv from cdna utilize mammalian cells, such as hep-2 or bsr t7/5, that are not currently suitable for vaccine manufacture. when using hep-2 cells, co-infection with an attenuated vaccinia virus that expresses t7 rna polymerase is also required. for human clinical trials, processes that ... | 2008 | 18722472 |
| dry cotton or flocked respiratory swabs as a simple collection technique for the molecular detection of respiratory viruses using real-time nasba. | this paper describes the molecular detection of influenza a, influenza b, respiratory syncytial virus and human metapneumovirus using real-time nucleic acid sequence based amplification (nasba) from respiratory samples collected on simple dry cotton swabs, non-invasively and in the absence of transport medium. viral rna was detectable on dry cotton and flocked swabs for at least 2 weeks at room temperature and was readily extracted using magnetic silica extraction methods. dry cotton respiratory ... | 2008 | 18761378 |
| a scoring system for management of acute pharyngo-tonsillitis in adults. | the aim of this study was to develop and evaluate a scoring system for the management of acute pharyngo-tonsillitis. | 2009 | 18774249 |
| [development and comparison of real-time and conventional rt-pcr assay for detection of human coronavirus nl63 and hku1]. | we designed specific primers and fluorescence-labeled probes to develop real-time and conventional rt-pcr assays for detection of human coronavirus nl63 or hku1. subsequently, experiments were undertaken to assess diagnostic criteria such as specificity, sensitivity and reproducibility. the detection limit of the real-time rt-pcr assays was 10 rna copies per reaction mixture. no cross-reactivity was observed between rna samples derived from designed hcov and other hcov or human metapneumovirus. ... | 2008 | 18780635 |
| multiple viral respiratory pathogens in children with bronchiolitis. | the aim of the study was to describe the frequency of viral pathogens and relative frequency of co-infections in nasal specimens obtained from young children with bronchiolitis receiving care at a children's hospital. | 2009 | 18785966 |
| the challenge of respiratory virus infections in hematopoietic cell transplant recipients. | respiratory virus infections in hematopoietic cell transplant (hct) recipients are a major cause of morbidity and mortality. while respiratory syncytial virus (rsv), human metapneumovirus, parainfluenzaviruses, and influenza viruses are well known for their potential to cause fatal pneumonia, information has only recently emerged regarding the significance of the newly discovered viruses, such as human coronaviruses nl63 and hku1, and human bocavirus. lymphopenia seems to be the most important r ... | 2008 | 18785968 |
| an alphavirus replicon-based human metapneumovirus vaccine is immunogenic and protective in mice and cotton rats. | human metapneumovirus (hmpv) is a recently discovered paramyxovirus that causes upper and lower respiratory tract infections in infants, the elderly, and immunocompromised individuals worldwide. here, we developed venezuelan equine encephalitis virus replicon particles (vrps) encoding hmpv fusion (f) or attachment (g) glycoproteins and evaluated the immunogenicity and protective efficacy of these vaccine candidates in mice and cotton rats. vrps encoding hmpv f protein, when administered intranas ... | 2008 | 18786987 |
| role of cellular glycosaminoglycans and charged regions of viral g protein in human metapneumovirus infection. | human metapneumovirus (hmpv) is an important cause of lower respiratory tract disease, particularly in infants and young children. hmpv has two major glycoproteins, g and f, which are responsible for virus attachment and membrane fusion, respectively. we investigated the role of cellular glycosaminoglycans (gags) and g protein in hmpv infection. the pretreatment of hmpv with soluble heparin markedly inhibited the infection of hep-2 cells. recombinant g protein, comprising the extracellular domai ... | 2008 | 18786997 |
| new respiratory viruses of humans. | acute respiratory viruses are a major cause of morbidity and mortality in humans worldwide and most acute respiratory infections are caused by viruses. many of these viruses cause the highest burden of disease in specific risk groups such as young infants, the elderly, and immune-compromised individuals. although the most important respiratory viruses of humans have been identified in the last century, in the past decade about a dozen "new" viruses have been discovered that may cause a high burd ... | 2008 | 18820582 |
| human metapneumovirus infection in adults. | human metapneumovirus (hmpv) was first identified in 2001 in dutch children with bronchiolitis. the virus is an rna virus in the pneumovirinae subfamily and is most closely related to respiratory syncytial virus. hmpv has been shown to have worldwide circulation with nearly universal infection by age 5. similar to influenza and respiratory syncytial virus, activity is greatest during the winter in temperate climates. most of the available data on the clinical manifestations of hmpv infection are ... | 2008 | 18820584 |
| viral pathogens in acute exacerbations of chronic obstructive pulmonary disease. | the objective of this study is to determine the prevalence of respiratory syncytial virus (rsv) and other viral respiratory pathogens in emergency department (ed) patients with acute exacerbations of chronic obstructive pulmonary disease (aecopd). copd patients presenting to the ed with <10 days of aecopd symptoms were eligible. we used pcr to test nasal swabs for common viral respiratory pathogens. we completed viral studies on 76 patients from two eds. patients had a mean age of 72 years, and ... | 2008 | 18825480 |
| mixed respiratory virus infections. | mixed respiratory viral infections are double negative common and evidence that they are associated with severe disease is supported by some groups. this controversial observation can be explained by the lack of sensitivity of the assessed methods used for viral identification and by the small number of patients included in the randomized cohorts studied. most studies showed that respiratory syncytial virus (rsv) is identified in about 70% of hospitalized infants with bronchiolitis during season ... | 2008 | 18829380 |
| human bocavirus infection in children with respiratory tract disease. | human bocavirus (hbov) is a ubiquitous, newly described member of the parvoviridae family frequently detected in the respiratory tract of children, but only few reports provide data proving the link between hbov and respiratory tract disease (rtd). | 2008 | 18833027 |
| inhibition of human metapneumovirus replication by small interfering rna. | human metapneumovirus (hmpv) is a major respiratory viral pathogen in young children, elderly individuals and immunocompromised patients. despite its major effects related to bronchiolitis, pneumonia and its potential role in recurrent wheezing episodes, there is still no commercial treatment or vaccine available against this paramyxovirus. | 2008 | 18839783 |
| clinical disease and viral load in children infected with respiratory syncytial virus or human metapneumovirus. | the relationship between respiratory syncytial virus (rsv) and human metapneumovirus (hmpv) quantity in respiratory secretions and severity of illness in children remains unclear. we assessed the effect of hmpv and rsv viral load as determined by reverse transcriptase polymerase chain reaction on disease characteristics. data were abstracted from medical records of 418 children with rsv and 81 children with hmpv; associations were evaluated in multivariate analyses, both continuously and compari ... | 2008 | 18842376 |
| [human metapneumovirus infection in children with acute respiratory tract inflammation in urumuqi]. | to understand whether human metapneumovirus (hmpv) is one of the pathogens leading to the children's respiratory infections in urumqi. | 2008 | 18843997 |
| first detection of human metapneumovirus in children with respiratory infections in romania. | the human metapneumovirus (hmpv) was first isolated in 2001 in the netherlands (van der hoogen and collaborators) from a nasopharyngeal aspirate sampled from an infant. based on the morphological, biochemical and genetic characteristics, the hmpv was initially classified in the genus metapneumovirus with the avian metapneumovirus (apv), the agent causing the respiratory infections of the upper tract in turkeys and other birds. subsequently, together with the respiratory syncytial virus (rsv), it ... | 2007 | 18928061 |
| human metapneumovirus nucleoprotein and phosphoprotein interact and provide the minimal requirements for inclusion body formation. | human metapneumovirus (hmpv) is a recently discovered paramyxovirus of the subfamily pneumovirinae, which also includes avian pneumovirus and human respiratory syncytial virus (hrsv). hmpv is an important cause of respiratory disease worldwide. to understand early events in hmpv replication, cdnas encoding the hmpv nucleoprotein (n), phosphoprotein (p), matrix protein (m), m2-1 protein and m2-2 protein were cloned from cells infected with the genotype a1 hmpv wild-type strain tn/96-12. hmpv n an ... | 2008 | 18931065 |
| effect of nmso3 treatment in a murine model of human metapneumovirus infection. | balb/c mice infected with human metapneumovirus (hmpv) were treated with the sulfated sialyl lipid nmso3 (one dose of 50 mg kg(-1)) given at the time of infection. nmso3 significantly reduced viral replication in the lungs, as well as hmpv-induced body weight loss, pulmonary inflammation and cytokine production, suggesting that antiviral treatment initiated at the beginning of viral infection can modify hmpv-induced disease. | 2008 | 18931066 |
| fatal hemorrhagic pneumonia caused by human metapneumovirus in an immunocompetent child. | 2008 | 18937761 | |
| biennial spring activity of human metapneumovirus in austria. | human metapneumovirus (hmpv) is considered an important respiratory pathogen in young children. to gain insight into the seasonality and epidemiologic characteristics of hmpv infection, this study determined the frequency of hmpv infections in hospitalized infants during a 7-year period. | 2008 | 18978517 |
| co-circulating genetically divergent a2 human metapneumovirus strains among children in southern taiwan. | an outbreak of human metapneumovirus (hmpv) among children in southern taiwan in 2004 prompted the investigation of the molecular epidemiology of hmpv from september 2003 to august 2005. respiratory specimens that were culture negative for a panel of respiratory viruses were examined for the presence of hmpv by rt-pcr. the results indicated that 59 out of 546 (10.8%) children were hmpv-positive. the majority of these hmpv-positive children were less than 2 years old (59.4%), females (61%), and i ... | 2008 | 19002556 |