Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| identification of specific fragments containing the 5' end of poliovirus rna after ribonuclease iii digestion. | the small protein (vpg) covalently linked to the 5' end of poliovirus type 1 (pv-1) rna has been labeled in vitro with 125i using the bolton and hunter reagent. the rna is not degraded under the conditions used and nearly all the label enters vpg and not the poly-nucleotide chain. when this 125i-labeled rna is cleaved with rnase iii at low monovalent salt concentrations, one major 125i-labeled fragment, approximately 100 nucleotides long, is produced. the corresponding fragment from similar dige ... | 1978 | 214765 |
| [method of milk processing for detecting the poliomyelitis virus]. | 1978 | 214962 | |
| viral pollution of surface waters due to chlorinated primary effluents. | the role of chlorinated primary effluents in viral pollution of the ottawa river (ontario) was assessed by examining 282 field samples of wastewaters from two different sewage treatment plants over a 2-year period. the talc-celite technique was used for sample concentration, and bs-c-1 cells were employed for virus detection. viruses were detected in 80% (75/94) of raw sewage, 72% (68/94) of primary effluent, and 56% (53/94) of chlorinated effluent samples. both raw sewage and primary effluent s ... | 1978 | 215085 |
| potential sources of error during virus thermal inactivation. | a review of virus thermal inactivation data published in the literature demonstrated variations in reported virus resistance. examination of the methods used indicated that numerous studies were made by heat processing virus suspensions in test tubes. duplication of some of the methods using milk suspensions of poliovirus 1 showed virus persistence after heating as a result of uneven temperature distribution inside the test tubes. unless the containers (preferably sealed ampoules or capillary tu ... | 1978 | 215086 |
| an enzymatic activity in uninfected cells that cleaves the linkage between poliovirion rna and the 5' terminal protein. | the 5' terminal protein (vpg) on poliovirion rna can be removed by cell-free extracts from a variety of uninfected cells. this soluble enzymatic activity is found in both nuclear and cytoplasmic extracts of hela cells and is activated by mg++. the enzyme activity cleaves the tyrosine-phosphate bond that links the protein to the rna. in a partially purified form it has insufficient nonspecific protease or nuclease activity to account for its action. the existence of this enzyme implies that polio ... | 1984 | 215328 |
| poliomyelitis in the fetus and the newborn. a comment on the new understanding of the pathogenesis. | a survey of the literature shows that although poliovirus may be recovered from fetuses whose mothers have paralytic poliomyelitis, there is no evidence that the fetuses themselves are affected. it is suggested that if postnatal poliomyelitis results from an autoallergic response not developed in the fetus, then poliovirus cannot enter the cns of the fetus. when a mother has paralytic poliomyelitis at delivery the neonate has a 40 per cent chance of poliomyelitis, with a case fatality rate of ab ... | 1979 | 215371 |
| peptide-chain initiation with lsc poliovirus is intrinsically more resistant to hypertonic environment than is peptide-chain initiation with mahoney virus and deletion mutants of mahoney virus. | peptide-chain initiation with lsc poliovirus was more resistant to hypertonic medium than peptide-chain initiation with mahoney poliovirus. protein synthesis of lsc virus retained its relative resistance to high osmolarity created by the addition of excess nacl to the medium in cells coinfected with mahoney virus. the data indicate that peptide-chain initiation with lsc virus is intrinsically more resistant to high osmolarity than that of mahoney virus rather than reflecting different permeabili ... | 1978 | 215776 |
| [poliomyelitis occuring in a previously vaccinated young woman with isolation of a coxsackie type a virus. report of a case]. | 1978 | 215970 | |
| cell-free synthesis and processing of the proteins of poliovirus. | poliovirus rna can be translated completely and accurately in rabbit reticulocyte lysates; the nascent poly-protein is processed to give primary products 1a, x, and 1b indistinguishable from those made in poliovirus-infected hela cells. the capsid precursor protein 1a is processed to form the capsid proteins vp0, vp1, and vp3, while the noncapsid precursor 1b is processed to form protein 2. | 1978 | 215997 |
| [several characteristics of new strains of diploid green marmoset fibroblasts]. | two strains of diploid fibroblasts were derived from green monkey fetal lungs and their main properties were studied. these strains were shown not to differ principally from the well studied human diploid fibroblasts. the derived cultures are free from contaminating viruses. the titers of attenuated poliomyelitis virus strains achieved in green monkey diploid cells are comparable to those in primary cultures of green monkey kidneys. the possiblity of using green monkey diploid fibroblasts in vir ... | 1978 | 216152 |
| high levels of microbial contamination of vegetables irrigated with wastewater by the drip method. | the public health aspects of the use of wastewater in agriculture and the effects of the drip irrigation method on the contamination of vegetables were studied. the method used was to simulate enteric microorganisms' dissemination by contaminated irrigation water in the field. the vegetables were irrigated with an effluent inoculated with a high titer of traceable microorganisms: poliovirus vaccine and a drug-resistant escherichia coli. the dissemination of the marker organisms in the field was ... | 1978 | 216306 |
| identification of detergents as components of wastewater sludge that modify the thermal stability of reovirus and enteroviruses. | the agent in wastewater sludge previously shown to reduce the heat required to inactivate reovirus (r. l. ward and c. s. ashley, appl. environ. microbiol. 34:681--688, 1977) was "separated" from other sludge components and analyzed by infrared spectroscopy. the infrared spectrum of this material was quite similar to the spectra of commercial anionic detergents, and subsequent analyses of the fractionated sludge samples revealed that anionic detergents in sludge were copurified with the virucidal ... | 1978 | 216308 |
| heat inactivation of enteric viruses in dewatered wastewater sludge. | the effect of moisture content on the rates of heat inactivation of enteric viruses in wastewater sludge was determined. the protective effect of raw sludge on poliovirus previously observed (r. l. ward, c. s. ashley, and r. h. moseley, appl. environ. microbiol. 32:339--346, 1976) was found to be greatly enhanced in sludge dewatered by evaporation. other enteroviruses responded in a similar fashion. this effect did not appear to be due merely to the state of dryness of the sludge samples because ... | 1978 | 216309 |
| isolation of viruses from sewage, with special regard to poliovirus: an evaluation of the sensitivity of three tissue culture methods. | this report concerns experiments to isolate different viruses from sewage. using a special cell-line from utrecht, derived from human amniotic cells, it was possible to isolate poliovirus selectively when antisera against six types of coxsackievirus b were added to the tissue culture. the method was tested in connexion with the epidemiological investigation of a case of poliomyelitis in sweden in 1977. it rapidly demonstrated that the virus implicated was present in all neighbouring sewage plant ... | 1978 | 216501 |
| fatal poliomyelitis with recovery of virus from cerebrospinal fluid. | 1978 | 216907 | |
| o4-(5'-uridylyl)tyrosine is the bond between the genome-linked protein and the rna of poliovirus. | virion rna of poliovirus type 1 has been analyzed for the linkage between genome-protein vpg and the polyribonucleotide chain. hydrolysis of the linkage with acid or alkali and enzymatic degradation lead to the conclusion that the bond is neither a phosphodiester such as nucleotidyl-(p-o)-serine (or threonine) nor a phosphoramidate such as nucleotidyl-(p-n)-amino acid. vpg-rna can be iodinated by the bolton and hunter reagent [iodinated 3-(4-hydroxyphenyl)propionic acid n-hydroxysuccinimide este ... | 1981 | 217003 |
| amino terminal sequence ambiguity in three capsid polypeptides of poliovirus. | 1983 | 217390 | |
| [melting and reassociation of double-stranded rna of the encephalomyocarditis virus]. | the processes of melting and reassociation of double-stranded rna in dimethylsulfoxide were studied. the addition of a small amount of licl results in great results in great reduction of tm (temperature of melting), whereas the nacl produces the opposite effect. it is suggested, that licl coordinates the molecules of h2o, reducing their activity, and consequently destabilises dsrna. mild conditions for melting and reassociation of rna can be created. it was found that under optimal conditions fo ... | 1979 | 217455 |
| enteric viruses in the danube river water and sludge. | a study of the viral pollution of the roumanian course of the danube river was carried out from 1972 to 1974. the presence of enteric viruses was investigated in 123 water samples, taken by the gauze swab method and in 116 sludge samples from the bottom of the river. the samples were collected from 20 sampling points along the river from moldova veche to brăila, in accordance with the water use and the main pollution sources. viruses were recovered from 17.5% of the samples. the frequency of vir ... | 1978 | 217928 |
| inhibition of a complete replication cycle of human cytomegalovirus in actinomycin pre-treated cells. | the study of human cytomegalovirus (hcmv) in cultures of human embryo lung fibroblasts, pre-treated with actinomycin d, has shown that under these conditions the virus infection does not proceed beyond the 'early' events of the virus replication cycle. in the same experimental conditions the growth of poliovirus type i, vaccinia virus and herpes simplex type i virus, was completely unaffected. these results suggest that the complete hcmv replication cycle requires some cellular function(s) betwe ... | 1979 | 217966 |
| growth of poliovirus type 1 in a non-primate cell line (ps cell) after artificial adsorption. | 1978 | 218082 | |
| an electron microscope study of the proteins attached to polio virus rna and its replicative form (rf). | a recently described method (wu, m. and davidson, n. (1978), nucleic acids research 5, in press) for visualizing proteins attached to nucleic acids in the electron microscope has been applied to study proteins attached to poliovirion rna and to the viral double-stranded intracellular rf form. a protein is found at the 5' end of the plus strand virion rna, and protein components are found at both ends of the duplex rf. in the rf as usually extracted, there is frequently a larger or compound prote ... | 1978 | 218168 |
| strand-specific attachment of avidin-spheres to double-stranded poliovirus rna. | poliovirus-specific double-stranded rna molecules containing covalently attached protein were coupled with a biotin ester through the protein moiety. subsequent interaction of the rna-biotin with avidin attached to electronopaque plastic spheres led to the formation of complexes that were easily visualized in the electron microscope. avidinspheres were associated only with one end of the rna-biotin molecules, as seen in the electron microscope. avidin-sphere attachment to poliovirus double-stran ... | 1979 | 218216 |
| [network for the epidemiological survey of the enteroviruses in sewage (author's transl)]. | poliomyelitis still occurs each year in france: 27 cases in 1973, 21 cases in each of 1974 and 1975, 8 cases in 1976. because of the risk, a survey of the spread of poliovirus in healthy populations and in sewage was organized by the ministry of health according to the departmental note dgs/hp/1st/250, 9-2-73. the laboratoire d'epidémiologie virale of the laboratoire national de la santé was in charge of the technical aspects of the survey. a preliminary questionnaire was sent to each d.d.a.s.s. ... | 1978 | 218261 |
| comparative studies on the genomes of some picornaviruses: denaturation mapping of replicative form rna and electron microscopy of heteroduplex rna. | 1979 | 218342 | |
| virus replication, cytopathology, and lysosomal enzyme response in enucleated hep-2 cells infected with poliovirus. | 1979 | 218347 | |
| epidemiological events surrounding a paralytic case of poliomyelitis in sweden. | a case of clinical poliomyelitis occurred in sweden in january 1977; it was the first indigenous case in sweden since 1962. this incident was of particular interest as it provided an opportunity to study the spread of virus in a cluster of unvaccinated persons and in individuals more or less fully vaccinated with inactivated vaccine. the patient excreted wild type 2 virus and the same type was isolated from 25 other individuals, all of whom were close contacts of virus excretors. among the close ... | 1979 | 218746 |
| intratypic serodifferentiation of poliomyelitis virus strains by strain-specific antisera. | strain-specific antisera against non-sabin-like and sabin-like poliovirus strains were prepared by cross-absorption of the antisera with the heterologous strain. isolates from different parts of the world were differentiated intratypically as non-sabin-like and sabin-like strains with the aid of these sera in the immunodiffusion test and neutralization test. in comparison with other marker tests, intratypic differentiation can be performed more easily. further, fewer intermediate strains were fo ... | 1979 | 218891 |
| restricted fragmentation of poliovirus type 1, 2 and 3 rnas by ribonuclease iii. | 1979 | 219203 | |
| defective interfering particles of poliovirus: mapping of the deletion and evidence that the deletions in the genomes of di(1), (2) and (3) are located in the same region. | 1985 | 219204 | |
| poliovirus polyuridylic acid polymerase and rna replicase have the same viral polypeptide. | a poliovirus-specific polyuridylic acid [poly(u)] polymerase that copies a polyadenylic acid template complexed to an oligouridylic acid primer was isolated from the membrane fraction of infected hela cells and was found to sediment at 4 to 5s on a linear 5 to 20% glycerol gradient. when the poly(u) polymerase was isolated from cells labeled with [(35)s]methionine and was analyzed by glycerol gradient centrifugation and polyacrylamide gel electrophoresis, the position of only one viral protein w ... | 1985 | 219230 |
| [adaptation of the type i poliomyelitis virus to cotton rats]. | adaptation of poliomyelitis virus type i (mahoney strain) to the cns of cotton rats which at the time of inoculation had been temporarily immunosuppressed with cyclophosphane is described. in the course of passages the virus properties were observed to change. the virus became pathogenic for cotton rats with normal immunologic responsiveness by the intracerebral, intraperitoneal and subcutaneous routes, as well as for white mice intracerebrally. virions pathogenic for cotton rats were predominan ... | 1979 | 219612 |
| [change in the level of immunoglobulins a in the intestines of children after administration of live trivalent poliomyelitis vaccine by different methods]. | it was shown that titers of the neutralizing activity of fecal extracts (coproantibody, ca) to poliomyelitis virus type ii in the course of four administrations of live trivalent poliovaccine in drops directly into the mouth and in capsules were higher than ca titers to poliomyelitis virus types i and iii. particularly low ca titers to poliovirus types i and iii were in children given the vaccine in the capsule, that is directly into the gastro-intestinal tract. accordingly iga levels in fecal e ... | 1978 | 219616 |
| [method of determination of the number of enteroviruses present in natural waters]. | the effectiveness of concentratin of enteroviruses present in water by adsorption on membrane filters, ion-exchange resin ab-17-8 and bentonite was compared with set concentrations of virus in water (0.05-0.1-0.5-2.5 and 12.5 pfu/ml). the effectiveness of concentration was shown to depend upon the initial dose of virus present in water. with virus adsorption on membrane filters and ionexchange resin the effectiveness of concentration was the higher (100 and 74%) the lower the virus dose in water ... | 1978 | 219620 |
| [recommendations for evaluation of interferon inducers of clinical importance]. | 1978 | 219621 | |
| [detection and persistence of human-pathogenic viruses in chemical sludge (author's transl)]. | virological investigations, using poliovirus type i, were carried out on the detection of enterovirus and their persistence in the chemical sludge from a tertiary treatment process, which could be suitable for agricultural and horticultural applications.--a comparison of six eluents showed that tryptose phosphate broth yielded best results allowing approximately 30% overall recovery with the method described. with sludge of low virus content a concentration was possible with alginate filters and ... | 1978 | 219641 |
| unsuitability of polioviruses as indicators of virological quality of water. | polioviruses could not be detected in about 50% of sewage samples despite routine administration of live attenuated poliovirus vaccine to the infant population. | 1979 | 219773 |
| seroimmunity to polioviruses in u.s. army recruits. | titers of neutralizing antibody to poliovirus types 1, 2, and 3 were determined for serum specimens obtained from 268 u.s. army recruits. among those tested, 20.9% lacked neutralizing antibody to one or more types of poliovirus, and 1.1% lacked antibody to all three types. an analysis of demographic data showed that age of less than 18 years, schooling for less than 10 years, and residence in the northeastern united states were associated with higher percentages of recruits lacking neutralizing ... | 1982 | 220335 |
| enterovirus infection in a semi-closed community. | infection with enteroviruses was studied over a 61-week period (during 1960-61) in a semi-closed child community in the detention home of the allegheny county juvenile court in pittsburgh, pennsylvania. while most of the viruses isolated were known adeno- and enterovirus types, three apparently represent new enterovirus types or 'prime strains'. viruses were isolated in all but 6 weeks of the 61-week study period from the gastro-intestinal tracts of 110 children out of a total population of 514 ... | 1979 | 220514 |
| failure to detect poliovirus genetic information in amyotrophic lateral sclerosis. | 1979 | 220904 | |
| csf viral antibodies. evaluation in amyotrophic lateral sclerosis and late-onset postpoliomyelitis progressive muscular atrophy. | serum and csf from 48 patients with amyotrophic lateral sclerosis and six patients with late-onset postpoliomyelitis progressive muscular atrophy were investigated for the presence of antibody to poliovirus types 1, 2, and 3, coxsackie viruses b3 and b4, influenza a, measles, rubella, mumps, herpes simplex types 1 and 2, cytomegalovirus, varicella-zoster, and toxoplasma gondii. these results were compared with those from 53 control patients with neuromuscular disease matched for age, sex, race, ... | 1979 | 220938 |
| regulation of glycolipid biosynthesis: effects of virus infection and drug-induced translational inhibition on glycolipid metabolism. | 1979 | 221008 | |
| [various problems in the molecular biology of picornaviruses]. | 1977 | 221803 | |
| translation of poliovirus rna in vitro: studies on n-formylmethionine-labeled polypeptides initiated in cell-free extracts prepared from poliovirus infected hela cells. | 1979 | 222055 | |
| degradation of poliovirus by adsorption on inorganic surfaces. | alteration of the specific infectivity of 3h-labeled ribonucleic acid and 14c-protein labeled poliovirus type 1 by adsorption on inorganic surfaces is investigated by application of kinetic theory to data obtained from sequential extractions of adsorbed virus. some surfaces, e.g., sio2, appear to have no significant effect. on the other hand, cuo substantially decreases the specific infectivity of adsorbed preparations. differences in kinetic plots between 3h-labeled ribonucleic acid and 14c-lab ... | 1979 | 222209 |
| variation in susceptibility of hela cell lines to coxsackievirus a9. | in the course of serial passages for several years a line of uncloned hela cells (a line) showed a gradual decrease in plaquing efficiency by coxsackievirus a9 (ca9 virus), while subcultures prepared from the same line kept frozen at an early passage level (a original line) did not show any change. however, it was observed later that the plaque-forming ability of the a original line (a orig. line) also decreased after serial passages as was observed with the a line. comparing the characteristics ... | 1979 | 222235 |
| fluorescence polarization of dph-labeled cells adsorbing viruses and its diagnostic potential. | mammalian or avian cells were labeled with a fluorescent probe dph (1,6-diphenyl-1,3,5-hexatriene). within a few minutes after adsorption of various naked and enveloped viruses, the degree of fluorescence polarization (p) of the dph embedded in the adsorbing cells as measured at 37 degrees c, was reduced, a finding indicating a decrease in the microviscosity of the lipids in the cell membrane. this change of fluidity was proportional to the concentration of the adsorbing virus and could be aboli ... | 1979 | 222236 |
| the cytoskeletal framework and poliovirus metabolism. | the cytoskeletal framework prepared by detergent lysis of suspension-grown hela cells is compared to the structure obtained from poliovirus-infected cells. this framework, which retains major features of cell morphology and carries the cellular polyribosomes as well as the major structural filaments, is profoundly reorganized following virus infection. this reorganization underlies, at least in part, the morphological changes termed the "cytoplasmic effect." these cytoskeletal changes appear rel ... | 1979 | 222454 |
| the introduction of poliovirus rna into cells via lipid vesicles (liposomes). | large unilamellar vesicles (luv) composed of phosphatidylserine are capable of encapsulating poliovirus ribonucleic acid (rna) and delivering it efficiently to cells in an infectious form. the biological activity of vesicle-entrapped poliovirus rna was 1-2 x 10(4) plaque forming units/nanogram (pfu/ng) and appeared to be enhanced by ribonuclease treatment of the vesicle preparations (infectivity = 1-2 x 10(5) pfu/ng). vesicle-mediated rna infection produced equivalent titers in primate and nonpr ... | 1978 | 222477 |
| assay of human interferon in vero cells by several methods. | four methods for the assay of human interferon in vero cells were compared based on the inhibition of viral cytopathic effect (cpe) in tubes, the inhibition of cpe in microplates, the reduction of plaques, and the inhibition of quantitative hemadsorption. for inhibition of cpe, sindbis virus, vesicular stomatitis virus, poliovirus type 2, and vaccinia virus were used for challenge. in the plaque reduction method, sindbis virus, vesicular stomatitis virus, and poliovirus were employed, and newcas ... | 1979 | 222803 |
| poliovirus replicase: a soluble enzyme able to initiate copying of poliovirus rna. | the soluble phase of the cytoplasm of poliovirus-infected cells contains an enzymatic activity able to copy rna without an added primer. this replicase activity has been purified 60-fold; it is absent from uninfected cells. poly(u) polymerase activity copurifies with replicase activity. although less pure replicase fractions copy a variety of rnas, purer fractions respond better to poliovirus rna than to other viral rnas. even the less pure fractions make a specific copy of the added template, a ... | 1979 | 223155 |
| virus-specified protease in poliovirus-infected hela cells. | previous studies have shown that primary cleavages in nascent picornavirus precursors are accomplished by cellular proteases. this study has characterized the enzyme in infected cells that produces the capsid polypeptides by secondary cleavages of viral precursors. the kinetics of the production of protease activity correlate with the time course of virus protein synthesis, and the new enzyme has characteristic ph and temperature optima. guanidine and cycloheximide, which are inhibitors of virus ... | 1979 | 223165 |
| [development of immunity to the poliomyelitis virus in infants with various methods of administering a live poliomyelitis vaccine]. | the data have been obtained which indicate that the effectiveness of vaccination with live trivalent poliomyelitis vaccine may be increased and 90--100% of the vaccinated infants under 1 year of age may develop marked resistance of the alimentary tract and high antibody titers in the blood serum to the threee types of poliomyelitis virus in the course of 3 vaccinations. some regularities of the formation of immunity to individual poliomyelitis virus types in the course of 4 vaccinations with tri ... | 1979 | 223328 |
| experiences of vaccination with inactivated poliovirus vaccine and epidemiology round a single case of polio in 1977 in sweden. | inactivated poliovaccine manufactured in this country has been used in sweden since 1957. the coverage of the vaccination in children is close to 100%. the majority of adults has also been immunized. no indigenous case occurred between 1962 and 1977. in 1977 a 2-year-old unvaccinated girl caught a mild paralytic disease caused by polio type 2. an epidemiologic investigation revealed excretion of virus in 25 persons, all close contacts to other excretors. no virus excretors were found among vacci ... | 1978 | 223905 |
| poliomyelitis survey in middle-east countries. | the poliomyelitis survey was started in 1976 in bahrain and kuwait. for each paralytic case, diagnosis was done by virus isolation, neutralizing antibody titration and specific igm characterization. more than 90% of the infants who were ill were less than 2 years old. in bahrain, 17 paralytic cases in 1976 and 12 in 1977 occurred between january and july (annual incidence was respectively 7.7 and 5.4/100,000). the first epidemic was due to poliovirus type 2, the second one to poliovirus type1. f ... | 1978 | 223907 |
| new approach to the production of concentrated and purified inactivated polio and rabies tissue culture vaccines. | the preparation of inactivated polio and rabies vaccine in tissue culture has been developed into a "unit process" at our laboratory during recent years. the process comprises trypsinization of animal kidney cells by the perfusion method, cell/virus cultivation in microcarrier culture and concentration and purification of the virus suspension followed by inactivation. also, the control tests on the virus suspensions and vaccines are performed, to the greatest possible extent, according to the sa ... | 1978 | 223908 |
| large-scale concentration and purification of virus suspension from microcarrier culture for the preparation of inactivated virus vaccines. | because of the presence of bovine serum proteins in poliovirus and rabiesvirus suspensions harvested from microcarrier cultures, a concentration and purification process has been developed in order to remove these proteins. the process comprises clarification by filtration, concentration by ultrafiltration, gel filtration on sepharose 6b and column chromatography on deae-sephadex a50. the serum protein content of the virus suspensions if reduced to a serum dilution of less than 1: 10(6) at a vir ... | 1979 | 223923 |
| purification of poliovirus obtained from human diploid cells by means of deae-sepharose. | mahoney, mef and saukett poliovirus strains were grown on human diploid cells (mrc 5) and concentrated on amicon filter. concentrated viral suspensions containing 3h-uridine labelled virus were mixed with 14c-amino acid labelled cell extracts containing calf serum and passed through a deae-sepharose column. fractions eluted at various ionic strengths were analyzed for infectivity, radio-activity and serum content by counter immunoelectrophoresis in the presence of a rabbit anti-calf serum. a pea ... | 1979 | 223925 |
| poliovirus-induced suppression of lymphocyte stimulation: a macrophage-mediated effect. | poliovirus was shown to suppress the in vitro response of human mononuclear blood cells stimulated with phytohaemagglutinin (pha), pokeweed mitogen (pwm), tuberculin purified protein derivative (ppd) or allogeneic cells. the suppression required infectious virus and the presence of macrophages. experiments with combined cultures of human lymphocytes stimulated with pha, pwm, ppd or allogeneic cells, and different numbers of autologous macrophages indicated that both lymphocyte stimulation and th ... | 1979 | 223973 |
| [structure of poliovirus genome rna and its biological significance (author's transl)]. | 1979 | 224117 | |
| capsid protein precursor is one of two initiated products of translation of poliovirus rna in vitro. | previous studies in our laboratory have demonstrated that cell-free systems translating the mahoney strain of poliovirus type i rna utilize two unique initiation sites. in this study, defective-interfering particles of poliovirus, which contain deletions in the region encoding the capsid proteins, are shown to initiate translation of proteins in vitro at these same two sites. both the standard virus and the defective-interfering virus rna direct the synthesis of two polypeptides labeled with n-f ... | 1979 | 224212 |
| cell-mediated immunity to polio and hla antigens in amyotrophic lateral sclerosis. | cell-mediated immunity to poliovirus was demonstrated in 21 of 33 patients suffering from amyotrophic lateral sclerosis (als), whereas no response to poliovirus was found in patients suffering from other neurologic disorders or in healthy controls. three of the severe bulbar cases produced a migration inhibition factor (mif) in the presence of poliovirus, although skin tests to common antigens were negative. an increased incidence (46 percent) of hla-a3 was found in patients with amytrophic late ... | 1979 | 224341 |
| inhibition of uncoating of poliovirus by arildone, a new antiviral drug. | 2003 | 224584 | |
| translation of poliovirus rna in vitro: changes in cleavage pattern and initiation sites by ribosomal salt wash. | 1979 | 224589 | |
| comparative monkey neurovirulence of sabin type iii poliovirus vaccines. | 1979 | 225329 | |
| inhibition of 80s initiation complex formation by infection with poliovirus. | anisomycin has been shown to stabilize ribosome initiation complexes containing messenger rna and met-trnaf met to high salt conditions. extracts from hela cells treated with 5 x 10(-7) m-anisomycin for 15 min accumulate 80s initiation complexes which can be detected by their absorbance in sucrose gradients. poliovirus-infected cells fail to form the 80s initiation complex early after infection, when inhibition of host cell protein synthesis occurs. these complexes re-form later in infection aft ... | 1979 | 225427 |
| fate of poliovirus during anaerobic digestion. | 1979 | 225573 | |
| lymphoblastic transformation in response to viral antigens in multiple sclerosis. | the blastogenic response of lymphocytes to pokeweed mitogen and a battery of viral antigens was studied in healthy individuals, patients with multiple sclerosis (ms), and patients with other neurologic disorders. the multiple sclerosis patients exhibited a diminished response to pokeweed mitogen and to mumps, parainfluenza, and poliomyelitis i antigens when compared with healthy individuals. however, there was no significant difference between the multiple sclerosis patients and the neurologic c ... | 1979 | 225695 |
| [studies on the incidence and genetic characteristics of polioviruses during vaccination with polyvalent vaccines]. | 1979 | 225761 | |
| isolation of poliovirus from cases of poliomyelitis in rio de janeiro, in 1975. | 2006 | 225773 | |
| method of soil column preparation for the evaluation of viral transport. | a method for packing soil columns to investigate viral transport phenomena is described. the columns were 10 cm in diameter and ranged from 33 to 100 cm in length. field conditions of the soil, including bulk density and profile, were reproduced in columns. ionic gradients resulting from sequential applications of wastewater and distilled water affected the movement of poliovirus i (chat) through soil. compared with 33-cm- and 66-cm-length columns, lower concentrations of infectious virions were ... | 1979 | 225992 |
| survival of poliovirus within organic solids during chlorination. | poliovirus in fecal homogenates was used to determine the protection against inactivation by chlorination afforded virus that was occluded within particulates. virus that was closely associated with or occluded within small fecal particulates was protected. a fourfold increase in combined residual chlorine was required to achieve the same degree of inactivation for occluded virus as for free or secondarily adsorbed virus. a combined chlorine residual of 6.6 mg/liter was necessary to achieve 50% ... | 1979 | 225993 |
| mechanisms of persistent infections by cytopathic viruses in tissue culture. brief review. | 1979 | 226039 | |
| virucidal activity of some quaternary ammonium compounds. | four different quaternary ammonium compounds were tested by microtechniques for evaluating their activity against the type species of six viral groups very frequently involved in human and animal pathology. three disinfectants showed good activity against five of the representative viruses under test, while the fourth proved less effective. poliovirus type 1 was found to be completely resistant. | 1978 | 226100 |
| choice of cell substrate for biological products. | 1979 | 227238 | |
| alterations in initiation factor activity from poliovirus-infected hela cells. | 1979 | 227856 | |
| genetical analysis on the stability of poliovirus type 3 leon 12a1b. | 1979 | 227906 | |
| pattern of plaquing efficiency at different temperatures (ppet) as a differentiation tool among poliovirus type 3 leon 12a1b progenies and its possible use as a genetic marker. | 1979 | 227907 | |
| guanidine-sensitive na+ accumulation by poliovirus-infected hela cells. | the na+ content of poliovirus-infected hela s3 cells increased during the late phase of virus replication, after virus inhibition of host cell protein synthesis and in coincidence with late viral functions. guanidine hydrochloride blocked the rise in na+ content, whereas the antiguanidine agent choline fully reversed the guanidine block. expression of one or more late viral functions was essential for na+ accumulation to occur because accumulation was inhibited by cycloheximide or guanidine adde ... | 1979 | 228053 |
| reactivity of neutralizing antibodies with different specificities to h particles of poliovirus. | in order to elucidate the antigenic structure of poliovirus, the reactivity of antibody produced with h antigenic particles of mahoney strain (polio type 1) was investigated. injection of h particles of mahoney strain into rabbits yielded neutralizing antibody as well as cf-n and cf-h antibodies. this result coincided with the report by hinuma and coworkers. neutralization tests with inhibitor resistant mahoney mutants revealed that the neutralizing antibody produced with h particles was of hn31 ... | 1979 | 228166 |
| [concentration of the poliomyelitis virus from water on fp-brand material]. | a method of filtration through fp cloth (petryanov's cloth) has been suggested for concentration of small virus quantities from large volumes of water. the effectiveness of virus removal from water of different degrees of contamination at virus concentrations of 0.05--0.1--0.5--2.5--12.5 pfu/ml was studied. about 88--100% of the initial virus content was removed from sewage, 80--90% from river water, 10--90% from tap water. when the effect of the eluting solutions for the removal of the virus re ... | 1979 | 228498 |
| [use of the vero transplantable cell line in a color test reaction for the quantitative determination of poliomyelitis virus-neutralizing antibodies]. | 1979 | 228500 | |
| n-alpha-cocoyl-l-arginine ethyl ester, dl-pyroglutamic acid salt, as an inactivator of hepatitis b surface antigen. | n-alpha-cocoyl-l-arginine ethyl ester, dl-pyroglutamic acid salt (cae), exhibited a strong inactivating effect on hepatitis b surface antigen. concentrations of cae required for 50 and 100% inactivation of the antigen were 0.01 to 0.025% and 0.025 to 0.05% respectively. cae completely inactivated hepatitis b surface antigen at the lowest concentration compared with various compounds including about 500 amino acid derivatives, sodium hypochlorite, 2,4,4'-trichloro-2'-hydroxydiphenyl ether, and so ... | 1979 | 228595 |
| factors involved in enzyme-linked immunoassay of viruses and evaluation of the method for identification of enteroviruses. | a quantitative enzyme-linked immunosorbent assay was used for identification of selected enteroviruses: poliovirus type 1, echovirus type 6, coxsackievirus a type 9, and coxsackievirus b types 1 through 6. partially purified viral antigens or virus-specific antibodies were adsorbed to polystyrene spectrophotometer cuvettes, which permitted the assays to be reported and compared in terms of enzyme units specifically reacting. both the adsorbed antigen and the adsorbed antibody methods were approx ... | 1979 | 229122 |
| determination of the length distribution of poly(a) at the 3' terminus of the virion rnas of emc virus, poliovirus, rhinovirus, rav-61 and cpmv and of mouse globin mrna. | rapid, detailed, and accurate analysis of the length spectrum of 3' terminal poly(a) in an rna population can be obtained by 3'-terminal 32p-labelling of rna with t4 rna ligase, digestion with ribonucleases t1 and a, and use of gel sequencing methods. length distributions of 3'-terminal poly(a) of emc virus, poliovirus, rhinovirus, rav-61, and cpmv virion rnas as well as mouse globin mrna are presented. | 1979 | 229465 |
| a convenient sequencing method for 5' protein-linked rnas. | a convenient nucleotide sequencing method for 5' end protein-linked rnas was developed. genome of lsc, 2ab poliovirus, which has a protein (vpg) covalently linked to the 5' terminus, was labelled with 125i bolton and hunter reagent after proteinase k treatment. no sign of labelling of nucleotide moiety in the genome with the reagent was detected. a labelled oligo peptide-linked ribonuclease t1 fragment was obtained from the 5' end of the genome. analysis of the complex by two dimensional gel ele ... | 1996 | 229466 |
| bioaccumulation and depuration of enteroviruses by the soft-shelled clam, mya arenaria. | low levels of feces-associated natural virus, simulating virus numbers estimated to exist in moderately polluted shellfish-growing waters, were used to evaluate the effectiveness of depuration as a virus depletion procedure in soft-shell clams. depuration effectiveness depended upon the numbers of virus bioaccumulated and whether virus was solids associated. virus uptake was greatest when viruses were solids associated and pollution levels were equivalent or greater than those likely to be found ... | 1979 | 229766 |
| mode of initiation of cell infection with sludge-associated poliovirus. | association with sludge solids did not allow poliovirus to enter cells other than through the normal receptor-mediated route. this implies that the infectivities of sludge-encapsulated virions are masked until their exposure permits binding to cell surface receptors. | 1979 | 229768 |
| rhinoviruses: kinetics of ultraviolet inactivation and effects of uv and heat on immunogenicity. | the kinetics of ultraviolet inactivation for two human rhinoviruses and poliovirus were compared. no major differences in the rates of ultraviolet inactivation were detectable. all viral preparations inactivated by ultraviolet irradiation induced neutralizing antibody in guinea pigs. in contrast, when guinea pigs were immunized with a heat inactivated rhinovirus preparation, little or no neutralizing antibody was elicited. immune electron microscopy of the heated rhinovirus preparations revealed ... | 1979 | 229797 |
| [transmission of poliovirus in the infantile population in cuba]. | 1997 | 229883 | |
| [levels of specific antibodies against poliomyelitis virus and degree of immune protection in umbria population]. | the authors studied neutralizing antibodies against poliomyelitis wild viruses in a sample of umbria population. the number of seronegative results for each virus strain was very low (less than 1%). the geometric mean titres were 21.07, 26.72, 19.13 for poliovirus types 1, 2, 3, respectively. the results of this seroepidemiological study shows that the umbria population is satisfactory protected against the poliomyelitis. | 1979 | 229884 |
| [method for isolation of enteroviruses from edible mollusca]. | a method for recovery of poliovirus from experimentally inoculated mussels has been evaluated. the method, based on adsorption-elution-acid precipitation of virus particles, allows a recovery of more than 93% of the added virus. | 1979 | 229885 |
| characterization of a new isolate of poliovirus defective interfering particles. | an independent isolate of poliovirus defective interfering particles has been analyzed. these particles, designated di(a), are apparently analogous to the di particles described by baltimore and co-investigators. electron microscopic heteroduplex analysis reveals that the di(a) isolate is a mixture of deletion mutants which changes with passage level. the di(a) population consists of at least five distinct deletion mutants, including one double deletion. electron microscopic mapping of the delet ... | 1979 | 229964 |
| immunization against poliomyelitis: risk/benefit/cost in a changing context. | benefit/risk factors in immunization against poliomyelitis are examined from the viewpoint of the relative risk and efficacy of killed and live poliovirus vaccines in the currently changing contexts of poliomyelitis prevalence in developed and developing countries. risk factors include virus of vaccine origin gaining access to the cns, and failure of the vaccine to immunize. data are presented to illustrate the degree to which the respective risks occur in developed and developing countries. the ... | 1979 | 230106 |
| antibody to poliovirus in older children and young adults in israel and the use of poliovaccines for the immunization of the seronegatives. | this report represents preliminary results of a more extensive study which is presently being carried out in israel. the main aims of this study were twofold: (1) to carry out a survey of the state of immunity of children of various ages and of teenagers to the three poliovirus types and (2) to compare the antibody response of the seronegatives to the two kinds of presently available poliovaccines, the oral poliovaccine (o. p. v., savin vaccine) and the inactivated poliovaccine (i. p. v., salk v ... | 1997 | 230108 |
| poliovirus antibodies in age groups: an assessment of obligatory vaccination in belgium. | since 1967, compulsory oral vaccination before 2 years of age has been nearly the only form of poliovaccination practiced in belgium, and there have been almost no cases of polio. in recent sera from 2,225 belgians, 2 year olds lacked demonstrable seroneutralizing antibody, in 31% for type 1, in 8% for type 2, and in 21% for type 3. the lack of antibodies grew as the children reached 11--12 years, at which age 65% lacked antibody for type 1, 15% for type 2, and 54% for type 3; only 19% had antib ... | 1979 | 230111 |
| sequence studies of poliovirus rna. iv. nucleotide sequence complexities of poliovirus type 1, type 2 and two type 1 defective interfering particles rnas, and fingerprint of the poliovirus type 3 genome. | the 32p-labelled genomes of poliovirus type 1, 2 and 3 have been digested with rnase t1 and the products separated by two-dimensional gel electrophoresis. all three fingerprints differ in the separation pattern of the large oligonucleotides. the molar yields of the large rnase t1-resistant oligonucleotides of type 1 and type 2 rna of poliovirus rna are close to one. by comparing the yields of these oligonucleotides to the amount of rna from which they originated, the chain length of type 1 polio ... | 1979 | 230285 |
| topographical studies on poliovirus capsid proteins by chemical modification and cross-linking with bifunctional reagents. | poliovirus capsid proteins comprise 15.1 lysines in vp1, 5.6 lysines in vp2, 11.7 lysines in vp3 and 5.5 lysines in vp4. treatment with monofunctional reagent n-succinimidyl 2,3-3h-proprionate leads to the modification of 3.4 lysines in vp1, 0.6 lysines in vp2, 2.0 lysines in vp3 and 0.03 lysines in vp4. chemical modification with the monofunctional reagent n-succinimidyl 3-(4-hydroxy,5-125i-iodophenyl)propionate results in a predominant labelling of vp1 and vp3, whereas vp2 is less accessible a ... | 1979 | 230294 |
| possible point mutation sites in lsc, 2ab poliovirus rna and a protein covalently linked to the 5'-terminus. | poliovirus type i, vaccine strain (lsc, 2ab), which is a temperature- and actinomycin d-sensitive mutant derived from type i mahoney strain, was grown in hela cells in the presence of 32p and a low concentration of actinomycin d. seven and a half h p.i., genome 32p-rna was recovered from the purified virion. analysis of rnase ti digests of the rna by two-dimensional gel electrophoresis revealed that three possible point mutation sites exist in the large and unique oligonucleotides in the fingerp ... | 1979 | 230298 |
| studies on antiviral glycosides, 4. inhibition of the multiplication of paramyxoviruses by phenyl-6-chloro-6-deoxy-beta-d-glucopyranoside. | the antiviral activity of phenyl-6-chloro-6-deoxy-beta-d-glucopyranoside (pcg) was studied. pcg specifically inhibited the growth of paramyxoviruses including sendai, measles and newcastle disease viruses in llcmk2 cells at a concentration of 0.5 to 1.0 mm, but did not restrict the multiplication of other rna viruses (influenza, vesicular stomatitis and polio viruses) at these concentrations. pcg might act in the late stage during virus replication of sendai virus as it did not inhibit virus rna ... | 1979 | 230307 |