Publications

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changes in peripheral plasma concentrations of progesterone, estradiol-17 beta, and luteinizing hormone during pregnancy and around parturition in the buffalo (bubalus bubalis). 19827152242
comparative biochemical and pathological studies on acute ruminal acidosis induced by molasses and grain feeding in buffalo calves (bubalus bubalis). 19827187819
serum lh concentrations in cyclic buffalo (bubalus bubalis).circulating lh levels of 14 murrah buffaloes were determined by a double-antibody radioimmunoassay using an homologous bovine system. at the onset of oestrus, the mean peak circulating lh level was 20.80 +/- 3.43 (s.e.m.) ng/ml in the hotter months (june-august) and 21.24 +/- 0.98 ng/ml during the cooler months (october-december), values remaining high for about 4 h. basal levels throughout the rest of the cycle averaged 1-2 ng/ml. conception occurred in 7 of the animals showing lh peaks (2/8 in ...19807191903
radioimmunoassay of cortisol in blood of buffaloes (bubalus bubalis) during the oestrous cycle. 19817198052
diurnal variations of progesterone in the milk of pregnant and non-pregnant buffaloes (bubalus bubalis). 19817198654
isolation of mycoplasma from a buffalo (bubalus bubalis) with mastitis. 19817199239
effects of climate on the thermoregulatory responses of male buffalo (bubalus-bubalis) calves supplemented with different levels of testosterone and tdn. 19827201981
ag-nors variation and banding homologies in two species of bovidae: bubalus bubalis and bos taurus.g,q and r-binding pattern comparisons between the murrah type of bubalus bubalis l. (2n - 50) and the holstein friesian breed of bos taurus l. (2n = 60) chromosomes revealed that the autosomes are similar in both species; in the former, the five pairs of submetacentrics correspond to centric fusions of chromosomes 1-29, 2-22, 8-19, 5-28 and 16-25 in the latter. silver staining on somatic cells of buffalo revealed telomeric ag-nors located on six pairs of autosomes, identified as 3p, 4p, 8, 21, 2 ...19817237236
a chromosomal analysis based on the g and c band staining techniques of the buffalo (bubalus bubalis). 19807253359
studies on the histogenesis of the tunica mucosa of the stomach of the egyptian water buffalo (bos bubalus l.).the histogenesis of the ruminal mucosa was studied in 9 buffalo fetuses (crl 90--730 mm) and one suckling female buffalo calf. the lamina epithelialis was found to consist of a basal and superficial layer. the former consists firstly of 2--4 cell layers and becomes later reduced to only one, made up to columnar cells; the latter one consists firstly of about 9 cell layers and increases then to 28--35, depending on the ruminal compartment. the epithelium shows its embryological feature, i.e. all ...19817258667
isolation of glycogen from buffalo liver.the glycogen has been isolated from buffalo (bos bubalus bubalis) liver, purified several-fold, and characterized to compare with rabbit and oyster glycogen. once-purified buffalo glycogen has been found to contain 1.3% protein, 0.16% nitrogen, 0.69% phosphorous, no lipids, and nucleic acids sufficient to cause absorption at 260 mu. the buffalo glycogen may be used as a potential substitute for rabbit and oyster glycogen after two-or three- purifications and a treatment with deae-cellulose.19807272087
ringer's lactate & homologous blood transfusion in experimental haemorrhagic shock in buffalo calves (bubalus bubalis). 19817275193
hexaxial reference system for frontal plane cardiac vectors in buffaloes bubalus bubalis. 19817309141
trypanosoma vivax in water buffalo (bubalus bubalis) of the amazon basin and the diagnosis of subpatent infection by anion exchange separation. 19817324118
muzzle secretion electrolytes as a possible indicator of sodium status in buffalo (bubalus bubalis) calves: effects of sodium depletion and aldosterone administration.in two separate experiments, the effects of sodium depletion and aldosterone administration on sodium and potassium concentrations in muzzle secretion, saliva and urine were studied in buffalo calves. sodium deficiency in the animals was experimentally produced by unilateral parotid saliva deprivation for 18 days. during sodium depletion, the sodium levels in saliva and muzzle secretion gradually fell while the potassium level gradually rose. the concentrations of both of these cations in urine ...19817340768
patho-anatomy of herniation of the reticulum through the diaphragm in the bovine.dissection of embalmed and untreated water buffalo carcasses (n=10) revealed that hernias had occurred at the musculotendinous junction of the diaphragm, ventral to the foramen venae cavae and slightly lateral to the median plane. the diameter of the hernial ring varied from 7 cm to 20 cm. herniation was more common in the right thoracic cavity with the reticulum firmly adherent to the hernia ring. adhesions between the herniated portion of the reticulum and pleura, lung, pericardium or thoracic ...19817340924
studies on the histogenesis of the tunica mucosa of the stomach of the egyptian water buffalo (bos bubalus l.). ii. histogenesis of the reticular mucosa.in the fetus with 9 cm crl the reticular mucosa was made up of a lamina epithelialis and a lamina propria/submucosa. the primordia of the reticular crests firstly appeared in the fetus with 16 cm crl as evaginations of the basal zone of the lamina epithelialis into the superficial one. invaginations from the luminal surface of the lamina epithelialis above the tips of the long reticular crests were formed in the fetus with 27 cm crl. aggregations of smooth muscle cells appeared in the cores of t ...19817342797
effect of spray and evaporative cooling on certain galactopoietic responses in buffaloes (bubalus bubalis).nine normal lactating murrah buffaloes from punjab agricultural university dairy herd were used to assess the effect of spray and evaporative cooling on certain galactopoietic responses in buffaloes in three equal groups of control (group i), showers (group ii) and evaporative cooling (group iii). at 10.30, 12.30, 14.30 and 16.30 h daily the animals in group ii were given showers for fifteen minutes while the animals of group iii were kept on evaporative cooling system from 07.00 to 21.00 h dail ...19817345888
a study on renal function in the indian buffalo (bubalus bubalis).glomerular filtration rate (gfr) and effective renal plasma flow (erpf) in buffalo species (bubalus bubalis) were estimated using a single injection technique. the total body clearances of inulin and para-aminohippuric acid (pah) served as estimates of gfr and erpf, respectively. inulin and pah were administered to animals as a single i.v. bolus. the time-concentration curves were determined for each compound. three mathematical models were applied to the data. the two compartment model gave the ...19817349346
on the leakage of acrosomal hyaluronidase from the spermatozoa of the buffalo (bubalus bubalis).the sonication method produced a quantitatively higher release of buffalo sperm hyaluronidase than the freeze-thaw technique. the released enzyme constituted 10 p. 100 of the total enzymatic activity of the fresh semen. seminal plasma hyaluronidase activity was not correlated with the motility score of the semen sample. unlike cattle semen, the seminal plasma enzyme level in buffalo semen stored at 37 degrees c showed a sharp rise, whereas samples stored at 0 degrees c evidenced negligible enzym ...19807349430
quantitative studies on spermatogenesis in buffalo (bubalus bubalis).the qualitative behaviour of spermatogenetic cells and sertoli cells in the buffalo (bubalus bubalis) was studied by identifying 8 stages of the seminiferous epithelium cycle based on meiotic division and morphology as well as the position of more mature spermatids. to achieve better results, stages 1, 2, 3, 4 and 8 were sub-divided. six peaks of spermatogonial mitosis suggested six generations of spermatogonia. of these, three were due to a (a1, a2, a3), two to b (b1, b2) and one to intermediat ...19807349465
primary structures of pancreatic ribonucleases from bovidae. impala, thomson's gazelle, nilgai and water buffalo.the amino acid sequences of the pancreatic ribonucleases from impala thomson's gazelle, nilgai and two types of water buffalo were studied from several enzymic digests. peptides were positioned by homology with other ribonucleases of known sequence. only peptides that differ in amino acid composition from the corresponding peptides of ox and goat ribonuclease were sequenced. the primary structures of pancreatic ribonucleases from 11 species of the bovidae family are known to date. the evolutiona ...19807353035
on the etiology of diaphragmatic hernia in buffaloes (bubalus bubalis). 19807366057
biochemical composition of microfiliarial and adult stages of setaria cervi.the microfilariae and adults of setaria cervi, a filarial parasite of indian water-buffalo (bubalus bubalis linn.) and of other ruminants were analysed for various biochemical constituents. both stages of the parasite were rich in protein, carbohydrate, and lipids while nucleic acids were detectable in only small amounts. microfilariae and adults contained 10 and 25% dry matter, 57.4 and 63.0% protein, 8.9 and 14.8% carbohydrate, and 12.8 and 9.2% lipid, respectively. reducing sugars accounted f ...19807395308
anatomical studies on the pudendal nerve in the indian buffalo (bubalus bubalis).this is a study of the origin, formation, course and distribution of the pudendal nerve (n. pudendus) in the indian buffalo (bubalus bubalis). the pudendal nerve originates from the union of the ventral rami of the 2nd, 3rd and 4th sacral spinal nerves. the largest contribution comes from the 3rd sacral nerve (13,284 +/- 844.45 fibres). the number of fasciculi, their area and arrangement, the total fibre count and the diameter spectra of the myelinated nerve fibres in n. pudendus and its rami ha ...19807396227
anatomical and neurohistological studies on the tongue of the indian buffalo (bubalus bubalis).in the indian buffalo (bubalus bubalis), the gross anatomical and neurohistological aspects of the tongue, its tunics and various lingual papillae have been studied. the observations indicate that the widest part of the tongue is also the thickest. the papillae encountered therein include filiform (four sub-types), fungiform and circumvallate papillae. simple branched tubulo-acinar serous glands are located in the lamina propria of the circumvallate papillae. the mucous glands are limited to the ...19807405526
study on the biokinetics of glucose metabolism using (2-3h) glucose during preruminant and ruminant stages in buffalo calves (bubalus bubalis). 19807424210
effects of some sugars, amino acids, glycerol and alpha-glycerophosphate on oxygen uptake of some digenetic trematodes.effect of various substrates on the oxygen uptake of some digenetic trematodes -- srivastavaia indica, gastrothylax crumenifer, gigantocotyle explanatum from the buffalo, bubalus bubalis and isoparorchis hypselobagri from the fish wallago attu was studied. in all the four species, the effect of various substrates except maltose was found to be stimulatory. the inhibitory or stimulatory effect of these substrates on oxygen uptake reflects the importance of these compounds in the energy metabolism ...19807450612
[the idiogram of the egyptian water buffalo (bubalus bubalis)]. 19807455281
[onchocerca sweetae occurring in the buffalo in thailand (author's transl)].the intradermal onchocerca from bubalus bubalis of thailand is the same as that which was described as o. sweetae from intradermal nodules in b. bubalis from australia. this identification suggests there has been no speciation between the parasites of australian and asiatic buffaloes. the taxon o. indica sweet 1915 consists of two distinct species; one in perimuscular nodules of bos indicus is most probably o. gibsoni (and thus, the species o. indica, based essentially on the description of this ...19807458162
a set of polymorphic dna microsatellites useful in swamp and river buffalo (bubalus bubalis).dna microsatellites have found widespread application in gene mapping, pedigree determination and population genetics. in closely related species such as bovids, heterologous polymerase chain reaction (pcr) primers may in some cases be used, bypassing the need to isolate and characterize microsatellite-containing sequences and design pcr primers. we report on the ability of a set of eighty bovine derived dna microsatellite primers to amplify sequences in the two types (swamp and river) of water ...19957486257
chromosomal evolution in bovids: a comparison of cattle, sheep and goat g- and r-banded chromosomes and cytogenetic divergences among cattle, goat and river buffalo sex chromosomes.a g- and r-banding comparison of cattle (bos taurus, 2n = 60), goat (capra hircus, 2n = 60) and sheep (ovis aries, 2n = 54) chromosomes at the 450 band level was made. the study revealed a large number of banding homologies among the autosomes of the three species and resolved some ambiguities in arranging some of their small disputed acrocentrics by direct and indirect comparisons with some bovid marker chromosomes. a loss of the subcentromeric g-positive band in sheep chromosome 2g was observe ...19957551543
evolution and recombination of bovine dna repeats.the history of the abundant repeat elements in the bovine genome has been studied by comparative hybridization and pcr. the bov-a and bov-b sine elements both emerged just after the divergence of the camelidae and the true ruminants. a 31-bp subrepeat motif in satellites of the bovidae species cattle, sheep, and goat is also present in cervidae (deer) and apparently predates the bovidae. however, the other components of the bovine satellites were amplified after the divergence of the cattle and ...19957563113
nucleotide diversity of mitochondrial dnas between the swamp and the river types of domestic water buffaloes, bubalus bubalis, based on restriction endonuclease cleavage patterns.cleavage patterns of mitochondrial dnas (mtdnas) by 15 restriction endonucleases were analyzed for 10 swamp and 13 river types of domestic water buffaloes. digestions with nine enzymes exhibited polymorphisms giving two or three kinds of cleavage patterns. five mtdna types were identified, three types in the swamp buffaloes of the philippines, vietnam, and indonesia (s-types) and two types in the river buffaloes of bangladesh and pakistan (r-types). nucleotide diversities ranged from 0.2 to 0.6% ...19957575390
assessment of amplicons in the dna from boiled tissue by pcr and ap-pcr amplification.polymerase chain reaction (pcr) is a technique sensitive enough to amplify small dna fragments a billion-fold. the generation of amplicons either by pcr with a set of oligo primers or by arbitrarily primed ap-pcr with a single oligonucleotide primer is based on the availability of intact template and priming sites. with these approaches, it is possible to generate specific and random amplicons to assess the extent of damage to dna caused by any of the physical, chemical, or environmental factors ...19957648472
identification of nucleolus organizer chromosomes in sheep (ovis aries l.) by sequential gbg/ag-nor and rbg/ag-nor techniques.seven gentile di puglia sheep (ovis aries l., 2n = 54) reared in southern italy were cytogenetically investigated to reveal which chromosomes carry nucleolus organizer regions (nors). the use of sequential gbg/ag-nor and rbg/ag-nor techniques in prometaphase preparations revealed nor-localization at the telomeres of chromosomes 1p, 2q, 3q, 6 and 25 on the basis of the standard r-banded karyotype. this procedure also permitted the characterization of g- and r-banding patterns of acrocentrics 6 an ...19937694824
purification of a 240 kda protein from serum and follicular fluid of water buffalo and its identification as haptoglobin.the fluids from healthy growing follicles of water buffalo were previously found free of the polypeptides h (m(r) 36,000) and l (m(r) 21,000) which were instead detected in fluids from atretic follicles and blood. here we report evidence that these two polypeptides, as selected from serum by specific anti-l antibodies, are the subunits of an oligomeric protein. the protein was purified from serum or follicular fluid, and its molecular weight (240 kda), isoelectric point (6.5), and amino acid com ...19957722477
sarcocystis fusiformis: some krebs cycle enzymes in various fractions of sarcocysts of buffalo (bubalus bubalis).a biochemical investigation was carried out on the relative presence of some enzymes of the krebs cycle and of the associated energy metabolism in various fractions (namely, cyst wall, cyst fluid and zoites) of sarcocysts of sarcocystis fusiformis from the oesophageal muscles of naturally infected indian water buffalo (bubalus bubalis). except for malate dehydrogenase, the activities of aconitase, isocitrate dehydrogenase, succinate dehydrogenase and fumarase were beyond detectable limits, point ...19957732635
an anonymous water buffalo probe detects a taqi rflp in cattle. 19947818189
pcr detection of ovine herpesvirus-2 dna in indonesian ruminants--normal sheep and clinical cases of malignant catarrhal fever.malignant catarrhal fever (mcf), a fatal viral disease of cattle and other large ruminants, has a worldwide distribution. there are two forms of the disease, one of which, is caused by alcelaphine herpesvirus-1 (ahv-1) and is derived from wildebeest. the other form is associated with domestic sheep and is caused by ovine herpesvirus-2 (ohv-2). the disease in indonesia is sheep-associated with the preferred livestock of this area, balinese cattle (bos javanicus) and water buffalo (bubalus bubalis ...19947839584
association of cumulus-oocyte complexes with the intrafollicular levels of a blood protein in bubalus bubalis.the protein pattern of the follicular fluid (ff) and the ultrastructure of the inner cumulus-oocyte complex (coc) has been analysed in single antral follicles (n = 146) of buffalo b. bubalis ovaries. the protein population of ff was fractionated by sds-page; the resulting pattern was coomassie stained and processed for densitometry. comparative analysis of sera and autologous ffs showed a marked difference in the level (measured as the percentage of total proteins) of one 21 kda polypeptide band ...19947874459
synaptonemal complex analysis of hybrid and purebred water buffaloes (bubalus bubalis).the morphology of the synaptonemal complex (sc) in river (2n = 50) and swamp (2n = 48) water buffaloes and their hybrids, was studied by electron microscopic analysis. in 2n = 49 hybrids, f2 and backcrosses the formation and pairing behaviour of a trivalent at zygotene-pachytene confirmed that river and swamp buffaloes differ by a centromere-to-telomere (c-t) tandem fusion. while 29% of spermatocytes from a purebred river buffalo and 16% from a purebred swamp buffalo had pairing abnormalities, a ...19947876032
localisation and capacitation-dependent loss of buffalo sperm-coating antigens shared with rat sperm.the heterodimeric sperm-coating protein cfs was previously localised on the middle-piece region of rat spermatozoa by anti-cfs rabbit antibodies. cfs-immunorelated antigens were detected in the secretion of the water buffalo seminal vesicle by protein electrophoresis and western blotting. spermatozoa from buffalo epididymal cauda were incubated with the rat antigen and, upon immunostaining with anti-cfs antibodies and goat anti-rabbit fluorescein isothiocyanate (fitc)-conjugated iggs, cfs was fo ...19947881916
recurrence of rift valley fever in egypt.rift valley fever (rvf) has been recorded in man and in domestic animals in egypt after a 12-year absence. human infections were first noted in the aswan governorate in late may, 1993. only cases of ocular disease, an infrequent and late manifestation, were reported. of 41 cases, 35 were tested serologically and 27 (77%) had rvf virus-specific igm antibodies. an estimated 600-1500 infections occurred in the region. abortions in cattle and buffalo were seen concurrently and antibodies to rvfv wer ...19937901480
chromosomal localization of conglutinin (cgn1) gene to river buffalo by sequential rbh-banding and fish. 19947928391
hereditary suprabasilar acantholytic mechanobullous dermatosis in buffaloes (bubalus bubalis).a skin disease characterized by trauma-induced sloughing of haired skin, hooves, and horns is described in four calves from a herd of murrah buffaloes (bubalus bubalis) in brazil. affected calves were detected shortly after birth by the presence of lesions affecting the distal extremities, the scapular and gluteal regions, and the tip of the tail. on histologic evaluation of affected skin, the lesions were characterized by suprabasilar vesicles and acantholysis affecting the epidermis and outer ...19947941234
an anonymous water buffalo probe detects rflp in sheep. 19947943957
genetic variants of ribosomal dna and mitochondrial dna between swamp and river buffaloes.to clarify the genetic relationship between swamp and river buffaloes, the restriction fragment length polymorphisms (rflps) of nuclear genomic ribosomal dna (rdna) and cytoplasmic mitochondrial dna (mtdna) were analysed. blood or liver samples from 73 swamp and three river buffaloes were collected in east and south-east asian countries. dna samples from cattle, goats and sheep were used for comparisons. the analysis of rdna allowed water buffaloes, cattle, goats and sheep to be characterized by ...19947943981
toxocara vitulorum: maternal transfer of antibodies from buffalo cows (bubalis bubalis) to calves and levels of infection with t vitulorum in the calves.the levels of antibody to the excretory/secretory antigens of the infective larvae and adults of toxocara vitulorum were measured by gel precipitation and elisas in the serum and colostrum of 12 buffalo cows naturally infected with t vitulorum and in the serum of their calves. the antibody levels were compared with the extent of t vitulorum infection as judged by faecal egg counts in the calves. the patterns of bands of the larval antigens and gel precipitating antibodies in cow serum taken one ...19947973098
the disposition of lead in blood and tissues of buffalo calves (bubalus bubalis). 19947975194
comparative pharmacokinetics of fenbendazole in buffalo and cattle.swamp buffalo (bubalus bubalis) and droughtmaster cattle (bos indicus x b. taurus), fitted with gastrointestinal cannulae, were dosed intraruminally with fenbendazole at 7.5 mg/kg liveweight, together with a chromium oxide capsule and a pulse dose of nacoedta, to estimate the flow dynamics of the digesta in the rumen and duodenum. the concentrations of fenbendazole (fbz) metabolites were measured in plasma and duodenal fluid collected over 120 h. in plasma, significantly lower peak concentration ...19947985383
some enzymes of gluconeogenesis in various fractions of sarcocysts of sarcocystis fusiformis of buffalo (bubalus bubalis).a biochemical study was conducted to assess the relative presence of some enzymes of gluconeogenesis in various fractions--the cyst wall, cyst fluid and zoites--of sarcocysts of sarcocystis fusiformis obtained from the oesophageal muscles of naturally infected indian water buffalo (bubalus bubalis). the activities of fructose 1,6-diphosphatase and malic enzyme were beyond detectable limits. phosphoenol pyruvate carboxykinase (pepck) activity was maximally present in the zoites, whereas glucose 6 ...19948030180
genetic studies of water buffalo blood markers. ii. carbonic anhydrase, esterase d, malate dehydrogenase, and malic enzyme. 19948031293
standard karyotype of the river buffalo (bubalus bubalis l., 2n = 50). report of the committee for the standardization of banded karyotypes of the river buffalo.during the 10th european colloquium on cytogenetics of domestic animals held in utrech, the netherlands, in 1992, an international committee on river buffalo chromosomes was established. at the recent 8th north american colloquium on domestic animal cytogenetics and gene mapping in guelph, canada (1993), q-, g- and r-banded river buffalo karyotypes were presented and discussed. this material was used to establish the first standard karyotype of the river buffalo.19948039419
the pathology of experimental trypanosoma evansi infection in the indonesian buffalo (bubalus bubalis).six indonesian buffaloes (bubalus bubalis) were inoculated intravenously with 10(5) trypanosoma evansi, examined clinically, haematologically and serologically, and then killed 1, 2, 3, 4, 8 or 12 weeks after infection for detailed pathological study. relapsing fever was related to the waves of parasitaemia and fluctuations of pulse and respiration rates. anaemic mucous membranes, depression, weakness, refusal to walk, loss of appetite and emaciation were seen. body weight, packed cell volume, t ...19948040389
strategies for purification of four reproductive hormones from the same batch of buffalo (bubalus bubalis) pituitaries.the occurrence of different reproductive hormones like lh, fsh, tsh and prolactin, in different side fractions obtained during the extraction of buffalo pituitary glands either by the procedure of papkoff et al. [arch biochem biophys, 111 (1965) 431] or by that of ellis [endocrinology, 69 (1961) 554], was examined with the aid of antisera to respective heterologous hormones as well as bio-assays. thus in the procedure of papkoff et al., the sp-sephadex fractions could be taken for purification o ...19948045616
localization of uridine monophosphate synthase (umps) gene to river buffalo chromosomes by fish.uridine monophosphate synthase plays an important role in pyrimidine synthesis, converting orotic acid to uridine 5' monophosphate. in cattle, umps deficiency is inherited as a monogenic autosomal recessive trait. while heterozygous carriers are phenotypically normal, homozygotes are lethal in utero (shanks et al. 1992). umps has been mapped to human chromosome 3q13 (qumsiyeh et al. 1989), sheep chromosome 1q (burkin et al. 1993) and cattle chromosome 1q31 (ryan et al. 1994). in the present stud ...19948069469
detection of genome specific monomorphic loci in bos taurus and bubalus bubalis with oligodeoxyribonucleotide probe.a synthetic oligodeoxyribonucleotide probe (oat36) comprising nine repeats of 5'gaca 3' and several enzymes were used to analyse cow, (bos taurus) and buffalo (bubalus bubalis) genomes and a number of monomorphic loci were detected in both the species. different animals from the same species showed an almost 'similar' monomorphic hybridization pattern but animals from two separate species showed a different 'genome specific' pattern. the overall hybridization with any enzyme and probe combinatio ...19938103300
a new centric fusion translocation in cattle, rob(16;18).a barrosã bull (portugal) has been found to carry a new robertsonian translocation involving chromosomes 16 and 18 of standard cattle karyotype, as demonstrated by gbg- and rbg-banding techniques. c-banding patterns revealed the dicentric nature of this translocation. a comparison between normal cattle chromosome 16 and the q-arms of translocation chromosome and river buffalo chromosome 5 revealed the same g- and r-banding patterns, with only exception of a pericentromeric g-positive band which ...19938144362
chromosomal localization of the lysozyme gene cluster in river buffalo (bubalus bubalis l.).lysozyme (lyz) is an antibacterial enzyme which allows the digestion of bacteria present in tears and saliva. in the true stomach of ruminants lyz breaks open the bacteria of the foregut, which are subsequently digested by typical mammalian digestive enzymes, allowing the incorporation of nutrients from the bacteria. southern analysis with a single exon from a cow lysozyme gene revealed that there are about 10 genes in ruminants (irwin & wilson 1989), while pig and primates have a single lysozym ...19938156163
trisomy-x in a sterile river buffalo. 19948197685
some blood parameters of water buffalo in different physiological conditions.the authors have studied the changes of got, gpt, alp, cpk and se in buffaloes under different physiological conditions (age, diet, different pre and post partum times). 99 adult buffaloes were classified in 8 groups on the basis of different pre, post partum time intervals. moreover, other 39 animals were divided into 4 groups: a = 60-90 day old animals, fed with reconstituted milk supplemented with vit e; b = same age, fed with maternal milk; c = 6-8 month old animals; d = 12-18 month old anim ...19938198807
the disposition of carbenicillin in blood, other body fluids and tissues of buffalo calves (bubalus bubalis). 19938212521
[morphostructural study of the lingual papillae in the buffalo (bubalus bubalus)].the morphostructural characteristics of lingual papillae in 30 adult buffaloes were studied by sem. in this species, dorsal lingual surface papillae shows different shape so that their classification is complicated. topography and morphology of conical and vallate papillae presents significant aspects. particularly, the conical papillae have a different morphological organization when they are localized over the side of the tongue apex or over dorso-lateral tract of the tongue body. the function ...19938238954
genetic studies of water buffalo blood markers. i. red cell acid phosphatase, albumin, catalase, red cell alpha-esterase-3, group-specific component, and protease inhibitor.we have developed the methodologies for typing and family studies to establish the modes of inheritance of water buffalo red cell acid phosphatase (acp), protease inhibitor (pi), and group-specific component (gc) on isoelectric focusing and albumin (alb), red cell alpha-esterase-3 (est-3), and catalase (cat) on polyacrylamide gel electrophoresis. family studies showed that pi, gc, alb, and cat are coded by autosomal genes with two codominant alleles, while est-3 is autosomal with two codominant ...19938259925
ultrastructural localization of wheat germ agglutinin binding sites on the sperm surface of water buffalo (bubalus bubalis). a fracture label study.in the present study we have examined the plasma membrane surface organization employing fluorescein isothiocyanate linked wheat germ agglutinin (wga) of the cauda epididymal and ejaculated spermatozoa of water buffalo. intramembrane particle distribution pattern in the various segments of the spermatozoa has also been observed. wga-ovomucoid gold has been used to study the distribution of sialoproteins on the sperm surface. with fracture label, wga receptor sites have been identified on the fra ...19938269396
sex determination of in vitro developed buffalo (bubalus bubalis) embryos by dna amplification.this study was conducted to determine the sex of buffalo embryos produced in vitro by amplifying male specific dna sequences using the polymerase chain reaction (pcr). this method uses three different pairs of bovine y-chromosome specific primers and a pair of bovine satellite specific primers. buffalo in vitro fertilized embryos at the 4-cell to blastocyst stage were collected at days 3, 4, 6, and 8 postinsemination, and the sex of each embryo was determined using all three different y-chromoso ...19938286109
[the possible pathways of substance transport in the muscle cysts of 2 species of sarcosporidia (sarcocystis, apicomplexa, sporozoa)].the present work is a sequel of our previous cytological investigations of the cyst-forming coccidia of the genus sarcocystis (reported elsewhere), now performed on two species from different hosts: s. muris (mouse) and sarcocystis sp. (water buffalo). acid phosphatase is synthesized in the endoplasmic reticulum of cystic zoites, its activity being seen in particular in the microenemes. as was established earlier (radchenko, 1991b), these organelles are extended regions of the smooth endoplasmic ...19938328021
chromosomal localization of the major histocompatibility complex in cattle and river buffalo by fluorescent in situ hybridization. 19938335474
chromosomal localization of omega and trophoblast interferon genes in goat and sheep by fluorescent in situ hybridization.fluorescent in situ hybridization (fish) of the cattle cdna probe btp-509 to goat (capra hircus l.) and sheep (ovis aries l.) r-banded metaphase chromosomes localized the omega (ifnw) and trophoblast (ifnt) interferon genes to goat chromosome 8q15 and sheep chromosome 2p15. caprine chromosome 8 and ovine chromosome 2p are equivalent in banding pattern to cattle chromosome 8. since fish has previously localized ifnw and ifnt genes to cattle chromosome 8q15 and river buffalo 3q15, the localization ...19938340618
purification and characterization of igg1 and igg2 from buffalo (bubalus bubalis) serum and colostrum.buffalo igg1 and igg2 were purified from serum and colostrum using salt precipitation, dialysis, gel filtration and ion-exchange chromatography. their purity was monitored by immunodiffusion and immunoelectrophoresis using anti-heavy chain specific sera and sds-page. selective binding of igg2 to protein-a was used to remove igg2 from igg1 preparations. the igg1 and igg2 had a molecular mass (mr) of 162.0 and 161.5 kd, respectively and were found to consist of heavy (h) and light (l) chains. the ...19938342260
distribution of some elements in hydatid cysts of echinococcus granulosus from buffalo (bubalus bubalis).distribution of some elements during development of endogenous daughter (hydatid) cysts of echinococcus granulosus of buffalo origin was determined by atomic absorption spectrophotometry. these analyses showed that the distribution of copper and cobalt was highest in the smallest cysts. these elements gradually decreased in the cysts as they enlarged. the distribution of zinc, iron and manganese was very high in the smallest cysts in comparison to copper and cobalt, but these three elements decr ...19938354855
electrospray mass spectrometric analysis of river buffalo (bubalus bubalis) hemoglobins. re-examination of alpha 1 and alpha 3 globin chain sequences.1. the accurate molecular weight of the globin chains from river buffalo hemoglobin components has been determined by means of electrospray mass spectrometry. 2. the es/ms analysis demonstrated that all the buffalo hb components share a common beta chain whose mass value coincides with that expected on the basis of the reported sequence. 3. the aa phenotype alpha 1 and alpha 3 globin chains exhibited a 30 da mass difference as compared to their predicted molecular weights. careful re-examination ...19938365113
spectroscopic and microscopic studies of buffalo-bull (bubalus bubalis) spermatozoa.we have examined the epididymal (caput, corpus and cauda) and ejaculated spermatozoa of bufallo-bull (bubalus bubalis) employing microscopic and spectroscopic techniques. fluorescein isothiocyanate conjugated lectins namely concanavalin a (con a), dolichos biflorus (dba), maclura pomifera (mpa), peanut agglutinin (pna), soybean agglutinin (sba) and wheat germ agglutinin (wga) were used to study the changes in the sperm surface carbohydrate make up as the spermatozoa mature. quantitative analysis ...19938384930
modification of fenitrothion-induced circulating enzymatic alterations in bubalus bubalis by 2,3-butanedione monoxime. 19938391347
effect of calmodulin-like protein from buffalo (bubalus bubalis) seminal plasma on ca2+, mg(2+)-atpase of purified plasma membrane of buffalo spermatozoa.buffalo sperm heads and tails were cleaved by sonication and isolated in relatively pure proportions i.e. 95% and 98% respectively, by discontinuous sucrose density-gradient centrifugation. purified plasma membranes from the isolated sperm heads and tails were obtained by hypotonic treatment and brief sonication followed by discontinuous sucrose density-gradient centrifugation. ca2+, mg(2+)-atpase activity was evident in plasma membrane from sperm heads and tails, although activity was greater i ...19938427418
studies on activation and levels of haemolytic complement of buffalo (bubalus bubalis). iii. c3 haemolytic activity in health and chronic disease.estimation of haemolytic complement component c3 activity in serum was done by using buffalo serum functionally depleted of c3, with methylamine. in the one-step alternate pathway (ap) haemolytic assay, c3 activity in the serum was estimated by its capacity to reconstitute the ap haemolytic activity in c3 depleted serum. levels of haemolytic c3 were determined in the sera of 70 apparently healthy buffaloes and of seven diseased buffaloes of which five were suffering from johne's disease (jd) and ...19938430503
chromosomal localization of omega and trophoblast interferon genes in cattle and river buffalo by sequential r-banding and fluorescent in situ hybridization.fluorescent in situ hybridization (fish) of the cattle cdna probe btp-509 to rba-banded cattle (bos taurus l.) chromosomes confirmed the assignment of the omega (ifnw) and trophoblast (ifnt) interferon genes to chromosome 8q15. using the same probe, these genes were also localized to river buffalo (bubalus bubalis l.) chromosome 3q15 following sequential rba-banding and fish. the extensive g- and r-banding homology observed between cattle chromosome 8 and river buffalo chromosome arm 3q supports ...19938440140
sarcocystis fusiformis: some protein metabolic enzymes in various fractions of sarcocysts of buffalo (bubalus bubalis).an investigation on the relative presence of some protein metabolic enzymes, namely aspartate aminotransferase (ast), alanine aminotransferase (alt), nad+ and nadp+ dependent glutamate dehydrogenase (gldh) and arginase in cyst wall (cw), cyst fluid (cf) and zoite (zt) fractions of the sarcocysts of sarcocystis fusiformis in the oesophageal muscles of indian water buffalo was carried out. both the transaminases were present in all the fractions of the cyst, although in variable amounts. there was ...19938447061
enterocin 226nwc, a bacteriocin produced by enterococcus faecalis 226, active against listeria monocytogenes.enterococcus faecalis 226, isolated from natural whey cultures utilized as starters in the manufacture of mozzarella cheese from water-buffalo milk, produces a bacteriocin designated enterocin 226nwc. the bacteriocin was isolated from culture supernatant fluids of the producer strain and was active against strains of the same species and listeria monocytogenes, but not against useful lactic acid bacteria. enterocin 226nwc is a protein with an apparent molecular weight of about 5800; it is relati ...19938486543
vitiligo in two water buffaloes: histological, histochemical, and ultrastructural investigations.vitiligo, a skin disease, characterized by the spontaneous loss of melanin, has been described in several animals as well as in humans. most of the reports of large domestic animals have dealt with clinical investigations without morphological data. in this report, the histological and ultrastructural characteristics of two cases of vitiligo in water buffaloes (bubalus bubalis) are presented. interestingly, many of the ultrastructural observations for vitiliginous buffaloes resemble those previo ...19938502622
purification and characterization of an alkaline proteinase from kidney cortex lysosomes.an alkaline proteinase was purified to apparent homogeneity from buffalo (bubalus bubalis) kidney cortex lysosomes by affinity chromatography on sti sepharose 4b and gel filtration over sephadex g-100. the molecular weight of the enzyme was 17,000 and 21,000 by gel filtration and sds/page respectively. the purified enzyme was optimally active at ph 8.5-9.0 at 50 degrees c and hydrolysed synthetic substrates of chymotrypsin but not those of elastase or trypsin. it was inhibited by serine proteina ...19938509124
the synlophe and other structural characteristics of sarwaria bubalis (nematoda: trichostrongyloidea) from cattle in guyana.the synlophe (longitudinal, surface cuticular ridges) of sarwaria bubalis is described for the first time. it is a tapering lateral synlophe of about 40 ridges. the synlophe of s. bubalis is similar to that of ostertagia ostertagi but markedly different from that of species of spiculopteragia and mazamastrongylus. new information is provided also on the structure of the esophagus and perivulval pores. the esophageal valve is more than twice as long as wide. the bilateral perivulval pores were lo ...19968627485
[the fertility of the water buffalo (bubalus bubalus)].in the present study the conception- and calving-frequencies of nili-ravi milk buffaloes were calculated over a year's period in the punjab region of pakistan. the results show prominent fluctuations throughout the year with a minimum calving-frequency of 1.6% in march and a minimum conception-frequency of 1.8% in may and maximum calving frequencies of 15.2% in november. this distribution occurs in association with unsuitable and suitable climatic conditions respectively, and also in association ...19958651897
behaviour of listeria monocytogenes during the traditional manufacture of water-buffalo mozzarella cheese.the behaviour of a four-strains mixture of listeria monocytogenes (strains scott a, v7, oh and cal) during the traditional manufacture of water-buffalo mozzarella cheese was investigated at two levels of inoculation: ca 10(5) and 10(3) cfu ml-1 of vat milk. no significant change in listeria counts was observed during the curd ripening (4.0-4.5 h), at the end of which the ph ranged between 4.83 and 4.91. a decrease of about 2 log was observed after stretching of the curd in hot water (95 degrees ...19968672274
detection of antibodies to staphylococcus aureus in water buffalo milk by flow cytometry.an assay has been developed to detect antibodies to staphylococcus aureus in water buffalo milk by flow cytometry. the method was the protein a-deficient strain wood 46 of s aureus incubated with milk samples and fluorescein-labelled rabbit anti-water buffalo antiserum. the assay can detect antibodies when the pathogen is not detectable by bacterial tests and can determine the antibody titre directly on undiluted samples.19968685542
liquid-phase blocking sandwich enzyme-linked immunosorbent assay for detection of antibodies against foot-and-mouth disease virus in water buffalo sera.to develop and apply the liquid-phase blocking sandwich elisa (blocking-elisa) for the quantification of antibodies against foot-and-mouth disease virus (fmdv) strains o1 campos, a24 cruzeiro, and c3 indaial. design--antibody quantification.19968725810
pharmacokinetics of radiolabelled buffalo follicle-stimulating hormone (125i-bufsh) in female buffalo calves (bubalus bubalis). 19968735427
kinetics of calcium transport through purified plasma membrane of buffalo (bubalus bubalis) spermatozoa: effect of calmodulin-like protein.a procedure is described for the isolation, identification, and purification of plasma membrane from buffalo ejaculated spermatozoa. a typical yield of plasma membrane vesicles obtained by this procedure was 280 micrograms of membrane protein per (10)9 spermatozoa. kinetics of calcium transport through purified plasma membrane vesicles were studied using 45ca2+. the transport of ca2+ through plasma membrane vesicles was electrogenic with maximum ca2+ uptake (47 mumol) occurring at 30 min in the ...19958777436
ovarian follicular dynamics during the estrus cycle in buffalo (bubalus bubalis). preliminary research. 19968784521
urinary excretion of purine derivatives and tissue xanthine oxidase (ec 1.2.3.2) activity in buffaloes (bubalis bubalis) with special reference to differences between buffaloes and bos taurus cattle.the urinary excretion of purine derivatives (pd) was measured in six buffaloes (bubalis bubalis) during fasting and in fourteen buffaloes given four restricted levels of roughage (2.5-4.8 kg dm/d). only allantoin and uric acid, not xanthine and hypoxanthine, were present in the urine, the pattern of excretion being similar to that in cattle. the fasting pd excretion amounted to 0.20 (sd 0.06) mmol/kg metabolic weight (w)0.75 per d, and the rate of pd excretion as a linear function of feed intake ...19968785213
fish mapping of the alpha-s2 casein gene on river buffalo and cattle chromosomes identifies a nomenclature discrepancy in the bovine karyotype.a mixture of five genomic clones spanning the alpha-s2 casein gene (csn1s2) were mapped to river buffalo (bubalus bubalis l.) and cattle (bos taurus l.) chromosomes by fluorescence in situ hybridization (fish) and r-banding. clear probe hybridization signals were detected on river buffalo chromosome 7q, band 32, and the homologous cattle chromosome. these mapping data allow the indirect assignment of the entire cattle u15 syntenic group to river buffalo chromosome 7. the assignment of u15 to riv ...19968785611
tumours of the urinary bladder in cattle and water buffalo in the black sea region of turkey. 19968791855
passive immune cross-protection in mice produced by rabbit antisera against different serotypes of pasteurella multocida.haemorrhagic septicaemia (hs) and fowl cholera (fc) are specific diseases caused by certain serotypes of pasteurella multocida. strains that usually cause hs in cattle and water buffalo do not produce fc in avian species, and strains that cause fc do not produce hs in cattle and water buffalo. a variety of p. multocida serotypes, including unusual strains which can cause hs in wild ruminants, were evaluated in passive immune protection studies to determine the immunological relationship between ...19968814530
ameloblastic fibromas and related tumors in cattle.this article concerns rare odontogenic tumors that occur predominantly in the mandibular incisor region of young cattle and which have often in the past been referred to as ameloblastomas, or as the outdated synonym, adamantinoma. twenty-two examples from the literature and two new ones were studied. six consisted of epithelial islands which resembled those of ameloblastoma but which were located within a cellular fibrous connective tissue that was the second component of the tumor; these mixed ...19968860142
synteny mapping in river buffalo.the cosegregation of ten coding loci has been investigated, in a panel of 37 somatic cell hybrids resulting from the fusion of a hamster cell line and river buffalo lymphocytes, by use of southern hybridization technique. five syntenic groups, tcrb-pgy3, ass-abl, fuca1p-cryg, mbp-yes1, and cgn1-acta1, previously assigned to cattle as u13, u16, u17, u28, and u29 respectively, were also found to be syntenic in buffalo. based on the extensive syntenic conservation and banding homology between cattl ...19968875891
phylogenetically conserved epitopes of leukocyte antigens.the occurrence of conserved epitopes in the immune system was investigated on the leukocytes of cattle, river buffalo, sheep, camel, swine and humans by indirect immunofluorescence and flow cytometry. the distribution of the most conservative epitopes on leukocyte sub-populations suggests that the expression pattern of the proteins is similar. western blotting experiments indicate that the recognized antigens are structural homologues.19968896233
assignment of genes coding for leukocyte surface molecules to river buffalo chromosomes.a panel of 35 somatic cell hybrids between river buffalo (bubalus bubalis l.) and chinese hamster was used in combination with 40 monoclonal antibodies to find synteny among leukocyte antigens and between already established synteny groups and leukocyte antigens. six synteny groups were identified and three sets of markers were related to already existing synteny groups. antibody il-a165 detected cd71, which is syntenic with synteny groups u10 and u25, confirming that u10 and u25 are both are on ...19968896235
mtx-block during s-phase in brdu-treated cells depends on species and medium used.stimulated peripheral blood cells from humans (homo sapiens), cattle (bos taurus), river buffaloes (bubalus bubalis), and sheep (ovis aries) were cultured in three different mediums, also differing in their respective quantities of folic acid. mtx (0.5 microgram/ml) and brdu (20 micrograms/ml) were simultaneously added to cell cultures 24 h before harvest and removed 18 h later. the mtx-block in late s-phase was checked by counting the number of cells which replicate once (g + c bands in both ch ...19968931357
isolation of mycobacterium bovis from the water buffalo (bubalus bubalis). 19958966765
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