Publications

dihedral angle study in hesperidin using nmr spectroscopy.	hesperidin is flavonoid molecule found in citrus fruits (citrus reticulata), especially difficult to extract, classify and characterize. present work is to study the unresolved relative configuration of hesperidin through the dihedral angle, coupling constant and different nmr techniques. the karplus equation and its modifications have been originated from the valence bond theory and associated with dihedral angle and coupling constant. the result data set of calculated dihedral angle can probe ...	2016	27943395
volatile and nonvolatile constituents and antioxidant capacity of oleoresins in three taiwan citrus varieties as determined by supercritical fluid extraction.	as local varieties of citrus fruit in taiwan, ponkan (citrus reticulata blanco), tankan (c. tankan hayata), and murcott (c. reticulate × c. sinensis) face substantial competition on the market. in this study, we used carbon dioxide supercritical technology to extract oleoresin from the peels of the three citrus varieties, adding alcohol as a solvent assistant to enhance the extraction rate. the supercritical fluid extraction was fractionated with lower terpene compounds in order to improve the o ...	2016	27999320
transcriptome profiling to understand the effect of citrus rootstocks on the growth of 'shatangju' mandarin.	to obtain insight into potential mechanisms underlying the influence of rootstock on scion growth, we performed a comparative analysis of 'shatangju' mandarin grafted onto 5 rootstocks: fragrant orange (citrus junons sieb. ex. tanaka), red tangerine (citrus reticulata blanco), 'shatangju' mandarin (citrus reticulata blanco), rough lemon (citrus jambhiri lush) and canton lemon (citrus limonia osbeck). the tree size of 'shatangju' mandarin grafted onto canton lemon and rough lemon were the largest ...	2017	28081213
restoration using azolla imbricata increases nitrogen functional bacterial groups and genes in soil.	microbial groups are major factors that influence soil function. currently, there is a lack of studies on microbial functional groups. although soil microorganisms play an important role in the nitrogen cycle, systematic studies of the effects of environmental factors on microbial populations in relation to key metabolic processes in the nitrogen cycle are seldom reported. in this study, we conducted a systematic analysis of the changes in nitrogen functional groups in mandarin orange garden soi ...	2017	28091792
comparative transcriptional survey between self-incompatibility and self-compatibility in citrus reticulata blanco.	seedlessness is an excellent economical trait, and self-incompatibility (si) is one of important factors resulting in seedless fruit in citrus. however, si molecular mechanism in citrus is still unclear. in this study, rna-seq technology was used to identify differentially expressed genes related to si reaction of 'wuzishatangju' (citrus reticulata blanco). a total of 35.67gb raw rna-seq data was generated and was de novo assembled into 50,364 unigenes with an average length of 897bp and n50 val ...	2017	28137595
chemical compositions, chromatographic fingerprints and antioxidant activities of citri exocarpium rubrum (juhong).	citri exocarpium rubrum (cer), which is known as juhong in chinese, is the dried exocarp of citrus reticulata blanco and its cultivars (fam. rutaceae) and is currently used in chinese medicine to protect the stomach and eliminate dampness and phlegm. the main aim of this study was to develop a high-performance liquid chromatography ultraviolet mass spectrometry (hplc-uv-ms) method for determining the chemical compositions and fingerprint of cer. we also evaluated the antioxidant properties of ce ...	2017	28138336
gaba pathway rate-limit citrate degradation in postharvest citrus fruit evidence from hb pumelo (citrus grandis) × fairchild (citrus reticulata) hybrid population.	organic acids are a major index of fresh fruit marketing properties. however, the genetic effects on the organic acid level in postharvest citrus fruit still remain unknown. here, we used the fruits of about 40 lines in a hybrid population (high-acid "hb pumelo" × low-acid "fairchild") to analyze the organic acid metabolism of postharvest citrus fruit. a transgressive content of titratable acid (ta) was observed, which was attributed to citrate accumulation. high- and low-acid fruits (no. 130, 1 ...	2017	28150945
largely different contents of terpenoids in beef red-flesh tangerine and its wild type.	niurouhong (citrus reticulata blanco. niurouhong) (nrh) is a spontaneous beef-red flesh mutant with distinctive flavor compared with its wild type orange-red flesh zhuhongju (zhj). to illustrate the biochemical mechanism of its special flesh color and flavor, fruits at commercial mature stage were used to profile the volatiles in the flavedo and determine the levels of carotenoids, limonoid aglycones and phytohormones in the juice sacs in two seasons.	2017	28158965
characterization of pseudomonas syringae pv. syringae, causal agent of citrus blast of mandarin in montenegro.	citrus blast caused by bacterium pseudomonas syringae is a very important disease of citrus occuring in many areas of the world, but with few data about genetic structure of the pathogen involved. considering the above fact, this study reports genetic characterization of 43 p. syringae isolates obtained from plant tissue displaying citrus blast symptoms on mandarin (citrus reticulata) in montenegro, using multilocus sequence analysis of gyrb, rpod, and gap1 gene sequences. gene sequences from a ...	2017	28167885
changes in anthocyanin production during domestication of citrus.	mandarin (citrus reticulata), citron (citrus medica), and pummelo (citrus maxima) are important species of the genus citrus and parents of the interspecific hybrids that constitute the most familiar commercial varieties of citrus: sweet orange, sour orange, clementine, lemon, lime, and grapefruit. citron produces anthocyanins in its young leaves and flowers, as do species in genera closely related to citrus, but mandarins do not, and pummelo varieties that produce anthocyanins have not been repo ...	2017	28196843
polyphenolic content and bactericidal effect of mexican citrus limetta and citrus reticulata.	in this study, total phenolics, total flavonoids, hesperidin and ascorbic acid contents in bagasse, juice and seed of mexican sweet lime (citrus limetta) and mandarine (citrus reticulata) were determined at two commercial maturity stages (maturation index), as well as their bactericidal effect on escherichia coli and staphylococcus aureus. the results showed that bagasses had the highest total phenolics, total flavonoids, and hesperidin content for both, c. limetta, and c. reticulata; highest as ...	2017	28242952
fast separation and sensitive quantitation of polymethoxylated flavonoids in the peels of citrus using uplc-q-tof-ms.	a rapid, sensitive, and efficient ultraperformance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (uplc-q-tof-ms) method has been developed to analyze polymethoxylated flavonoids (pmfs) in 14 citrus peels, including 7 citrus reticulata (c. reticulata) and 7 citrus sinensis (c. sinensis). in this study, fast separation can be achieved within 12 min and 42 pmfs have been identified including 33 flavones and 9 flavanones. most c. reticulata were shown to contain more ...	2017	28260382
evaluation of bioactive flavonoids and antioxidant activity in pericarpium citri reticulatae (citrus reticulata 'chachi') during storage.	a simple and accurate method using high performance liquid chromatography (hplc) with dual wavelength detection was developed to simultaneously determine the contents of one flavanone glycoside (hesperidin) and five polymethoxylated flavones (pmfs: sinensetin, 4',5,7,8-tetramethoxyflavone, nobiletin, tangeretin and 5-o-desmethyl nobiletin) in pericarpium citri reticulatae (citrus reticulata 'chachi') ('chachi' pcr). by modifying the mobile phase compositions and detection wavelengths, an optimal ...	2017	28407962
a wireless sensor network for growth environment measurement and multi-band optical sensing to diagnose tree vigor.	we have tried to develop the guidance system for farmers to cultivate using various phenological indices. as the sensing part of this system, we deployed a new wireless sensor network (wsn). this system uses the 920 mhz radio wave based on the wireless smart utility network that enables long-range wireless communication. in addition, the data acquired by the wsn were standardized for the advanced web service interoperability. by using these standardized data, we can create a web service that off ...	0	28448452
quantification of transfer of salmonella from citrus fruits to peel, edible portion, and gloved hands during hand peeling.	although studies have quantified bacterial transfer between hands and various materials, cross-contamination between the surface of fresh citrus fruit and the edible portions during hand peeling has not been reported. this study quantifies transfer of salmonella to the edible portion of citrus fruit from a contaminated peel during hand peeling. citrus fruits used for this study were citrus sinensis (sweet orange) cultivars 'valencia' and 'navel', citrus unshiu (satsuma mandarins), citrus reticul ...	2017	28463082
fermented citrus reticulata (ponkan) fruit squeezed draff that contains a large amount of 4'-demethylnobiletin prevents mk801-induced memory impairment.	a previous study reported biotransformation of a citrus peel polymethoxyflavone, nobiletin, by aspergillus enabling production of 4'-demethylnobiletin, and the product's antimutagenic activity. however, the effects of fermented citrus peel on the basal forebrain-hippocampal system remain unidentified. citrus reticulata (ponkan) fruit squeezed draffs are generated as mass waste in beverage factories. in this study using pc12d cells and cultured central nervous system neurons, we therefore examine ...	2017	28488113
comparative transcriptome analyses of a late-maturing mandarin mutant and its original cultivar reveals gene expression profiling associated with citrus fruit maturation.	characteristics of late maturity in fruit are good agronomic traits for extending the harvest period and marketing time. however, underlying molecular basis of the late-maturing mechanism in fruit is largely unknown. in this study, rna sequencing (rna-seq) technology was used to identify differentially expressed genes (degs) related to late-maturing characteristics from a late-maturing mutant 'huawan wuzishatangju' (hwwzstj) (citrus reticulata blanco) and its original line 'wuzishatangju' (wzstj ...	2017	28533983
in silico identification and validation of potential micrornas in kinnow mandarin (citrus reticulata blanco).	micrornas (mirnas) are a large family of 19-25 nucleotides, regulatory, non-coding rna molecules that control gene expression by cleaving or inhibiting the translation of target gene transcripts in animals and plants. despite the important functions of mirnas related to regulation of plant growth and development processes, metabolism, and abiotic and biotic stresses, little is known about the disease-related mirna. here, we present a new pipeline for mirna analysis using expressed sequence tags ...	2017	28534166
essential and toxic element concentrations in monofloral honeys from southern croatia.	the concentrations of 24 elements in seven honey types (multifloral, heather, common heather, bearberry, sage, mandarin orange-blossom and honeydew) collected in southern mediterranean regions of croatia were determined using icp-ms. significant differences were found in the concentrations of ag, as, ba, cu, co, fe, k, mg, mn, mo, na, ni, se, sb, u and th (p<0.05, all) among honeys. the highest element concentrations were determined in honeydew honeys, with the exception of multifloral (ca, cr, ...	2017	28551232
polymethoxyflavones in peel of citrus reticulata 'chachi' and their biological activities.	citrus polymethoxyflavones (pmfs) have been of increasing interest due to their extensive biological activities. in the present study, a total of eight pmfs were isolated from the peel of citrus reticulata 'chachi' (crc). they were individually identified as 5-hydroxy-6,7,8,4'-tetramethoxyflavone (1), 5,6,7,3',4'-pentamethoxyflavanone (2), 5-hydroxy-6,7,8,3',4'-pentamethoxyflavone (3), 3,5,6,7,8,3',4'- heptamethoxyflavone (4), 5,6,7,8,3',4'-hexamethoxyflavone (5), 5,6,7,8,4'-pentamethoxyflavone ...	2017	28551233
five new limonoids from peels of satsuma orange (citrus reticulata).	five new: 21,23-dihydro-21-hydroxy-23-oxonomilin (1), 21,23-dihydro-23-methoxy-21-oxonomilin (2), 21,23-dihydro-21-hydroxy-23-oxonomilinic acid methyl ester (3), 21,23-dihydro-23-methoxy-21-oxolimonin (4), and 21,23-dihydro-21-oxolimonin (5), and seven known limonoids were isolated from peels of satsuma orange (citrus reticulata). the isolated compounds were evaluated for their inhibitory effects on macrophage activation by an inhibitory assay of nitric oxide (no) production. among them, compoun ...	2017	28561764
cytotoxic properties of the stem bark of citrus reticulata blanco (rutaceae).	the bioassay-guided fractionation of the n-hexane extract of citrus reticulata blanco (rutaceae) stem bark yielded scoparone (1), xanthyletin (2), lupeol (3), β-amyrin (4), stigmasterol (5), β-sitosterol (6) and palmitic acid. the structures of these compounds were determined by comprehensive spectroscopic analyses, i.e., 1d and 2d nmr and ei-ms, and by comparison with the reported data. extracts, fractions and isolated compounds 1-6 were assessed for cytotoxicity by the 3-(4,5-dimethylthiazol-2 ...	2017	28568378
effect of mandarin orange yogurt on allergic conjunctivitis induced by conjunctival allergen challenge.	to evaluate the effects of mandarin orange yogurt containing nobiletin and β-lactoglobulin on the allergic conjunctivitis induced by a conjunctival allergen challenge (cac).	2017	28593244
effect of applying cinnamaldehyde incorporated in wax on green mold decay in citrus fruits.	green mold caused by penicillium digitatum is the most damaging postharvest diseases of citrus fruit. cinnamaldehyde (ca) is a food additive that has potential use in controlling postharvest disease of fruits and vegetables. in this study, the effectiveness of wax with ca (wca) in controlling ponkan (citrus reticulata blanco) green mold was investigated.	0	28620947
antioxidant capacity, anticancer ability and flavonoids composition of 35 citrus (citrus reticulata blanco) varieties.	citrus (citrus reticulate blanco) is one of the most commonly consumed and widely distributed fruit in the world, which is possessing extensive bioactivities. present study aimed to fully understand the flavonoids compositions, antioxidant capacities and in vitro anticancer abilities of different citrus resources. citrus fruits of 35 varieties belonging to 5 types (pummelos, oranges, tangerines, mandarins and hybrids) were collected. combining li quid chromatography combined with electrospray io ...	2017	28678176
an r2r3-myb transcription factor represses the transformation of α- and β-branch carotenoids by negatively regulating expression of crbch2 and crnced5 in flavedo of citrus reticulate.	although the functions of carotenogenic genes are well documented, little is known about the mechanisms that regulate their expression, especially those genes involved in α - and β-branch carotenoid metabolism. in this study, an r2r3-myb transcriptional factor (crmyb68) that directly regulates the transformation of α- and β-branch carotenoids was identified using green ougan (mt), a stay-green mutant of citrus reticulata cv suavissima. a comprehensive analysis of developing and harvested fruits ...	2017	28681945
flavonoid composition of tarocco (citrus sinensis l. osbeck) clone "lempso" and fast antioxidant activity screening by dpph-uhplc-pda-it-tof.	clonal selection and hybridisation are valid strategies to obtain fruits with enhanced sensorial and nutraceutical properties. within citrus sinensis varieties, tarocco clone "lempso" is a typical product of the calabria region (italy) characterised by its red pulp. this is the first report concerning its accurate profiling.	2017	28686312
comparative analysis of juice volatiles in selected mandarins, mandarin relatives and other citrus genotypes.	citrus fruit flavor is an important attribute prioritized in variety improvement. the present study compared juice volatiles compositions from 13 selected citrus genotypes, including six mandarins (citrus reticulata), three sour oranges (citrus aurantium), one blood orange (citrus sinensis), one lime (citrus limonia), one clementine (citrus clementina) and one satsuma (citrus unshiu).	2018	28731231
unreduced megagametophyte production in lemon occurs via three meiotic mechanisms, predominantly second-division restitution.	unreduced (2n) gametes have played a pivotal role in polyploid plant evolution and are useful for sexual polyploid breeding in various species, particularly for developing new seedless citrus varieties. the underlying mechanisms of 2n gamete formation were recently revealed for citrus reticulata but remain poorly understood for other citrus species, including lemon (c. limon [l.] burm. f.). here, we investigated the frequency and causal meiotic mechanisms of 2n megagametophyte production in lemo ...	2017	28747921
antimicrobial spray nanocoating of supramolecular fe(iii)-tannic acid metal-organic coordination complex: applications to shoe insoles and fruits.	numerous coating strategies are available to control the surface properties and confer new properties to substrates for applications in energy, environment, biosystems, etc., but most have the intrinsic limitations in the practical setting: (1) highly specific interactions between coating materials and target surfaces are required for stable and durable coating; (2) the coating of bulk substrates, such as fruits, is time-consuming or is not achievable in the conventional solution-based coating. ...	2017	28765556
epidemiologic characteristics of scrub typhus on jeju island.	scrub typhus is the most common febrile disease in korea during the autumn. jeju island is the largest island in south korea and has a distinctive oceanic climate. this study aimed to identify epidemiologic characteristics of scrub typhus on jeju island.	2017	28823118
identification of protoplast-isolation responsive micrornas in citrus reticulata blanco by high-throughput sequencing.	protoplast isolation is a stress-inducing process, during which a variety of physiological and molecular alterations take place. such stress response affects the expression of totipotency of cultured protoplasts. micrornas (mirnas) play important roles in plant growth, development and stress responses. however, the underlying mechanism of mirnas involved in the protoplast totipotency remains unclear. in this study, high-throughput sequencing technology was used to sequence two populations of sma ...	2017	28829800
identification of qtls controlling aroma volatiles using a 'fortune' x 'murcott' (citrus reticulata) population.	flavor is an important attribute of mandarin (citrus reticulata blanco), but flavor improvement via conventional breeding is very challenging largely due to the complexity of the flavor components and traits. many aroma associated volatiles of citrus fruit have been identified, which are directly related to flavor, but knowledge of genetic linkages and relevant genes for these volatiles, along with applicable markers potentially for expeditious and economical marker-assisted selection (mas), is ...	2017	28830354
optimization of low power ultrasound-assisted extraction of phenolic compounds from mandarin (citrus reticulata blanco cv. sainampueng) peel.	mandarin peel is a good source of phenolic compounds, which can be extracted by the ultrasound-assisted extraction (uae) method. this research was to optimize the uae conditions for maximum mandarin peel extract (mpe) relating to the extract yield, total phenolic content and the content of a mandarin peel rich flavonoid, hesperidin, using a response surface method comparing with the maceration extraction (mae) method. the results showed that the selected factors (temperature, time and power) hav ...	2018	28958537
impact of in vitro digestion phases on the stability and bioaccessibility of carotenoids and their esters in mandarin pulps.	the composition of carotenoids (carotenes and free and acylated xanthophylls) and their bioaccessibilities were determined for the first time in pulps of mandarins cultivated in brazil. two cultivars of mandarin, citrus reticulata blanco cv. 'ponkan' and citrus reticulata × c. sinensis cv. 'murcott', showed higher contents of most carotenoids compared to those found in c. deliciosa tenore cv. 'rio'. the major carotenoids in mandarin cv. 'ponkan' and 'murcott' were (all-e)-β-cryptoxanthin laurate ...	2017	28972218
chemical composition of the essential oils isolated from peel of three citrus species and their mosquitocidal activity against culex pipiens.	three essential oils (eos) were isolated from the peel of citrus fruits citrus reticulata l., citrus reticulata chinase blanco and citrus sinensis (l.) osbeck (family: rutaceae) and evaluated against culex pipiens l.1758 (family: culicidae). chemical composition indicated that the eos were rich in essential phytochemicals including hydrocarbons, monoterpenes and sesquiterpenes. these constituents revealed some variability among the oils displaying interesting chemotypes limonene (35-51%), 1r-α-p ...	2017	29017021
management of fruit species in urban home gardens of argentina atlantic forest as an influence for landscape domestication.	home gardens are considered germplasm repositories and places for experimentation, thus they are key sites for the domestication of plants. domestication is considered a constant process that occurs along a continuum from wild to managed to domesticated populations. management may lead to the modification of populations and in other cases to their distribution, changing population structure in a landscape. our objective is focused on the management received in home gardens by perennial species o ...	2017	29033964
effect of citrus reticulata blanco essential oil on cryptolestes ferrugineus (stephens) adults.	the rusty grain beetle, cryptolestes ferrugineus (stephens), is a major insect pest of stored products worldwide. in an effort to manage populations of c. ferrugineus in stored grains, the essential oil from citrus reticulata blanco peel was prepared by the soxhlet method with anhydrous diethyl ether, and its contact activity, repellent activity, and fumigant activity were investigated against c. ferrugineus adults. c. reticulata peel essential oil had potent contact, repellent, and fumigant act ...	2017	29154719
processing of wet kinnow mandarin (citrus reticulata) fruit waste into novel brønsted acidic ionic liquids and their application in hydrolysis of sucrose.	in citrus processing industries, where up to 60% of the whole fruit is discarded, generates citrus waste (peel, seeds, membrane, and pulp) on a massive scale. the waste does not currently have high-value applications, instead the majority is disposed of or pelletized for animal feed. therefore, a concise and efficacious protocol for processing of kinnow mandarin (citrus reticulata) fruit waste to numerous novel brønsted acidic ionic liquids (3a-k) have been developed. bails were characterized us ...	2017	29220805
differentiation between flavors of sweet orange (citrus sinensis) and mandarin (citrus reticulata).	pioneering investigations referring to citrus flavor have been intensively conducted. however, the characteristic flavor difference between sweet orange and mandarin has not been defined. in this study, sensory analysis illustrated the crucial role of aroma in the differentiation between orange flavor and mandarin flavor. to study aroma, valencia orange and lb8-9 mandarin were used. their most aroma-active compounds were preliminarily identified by aroma extract dilution analysis (aeda). quantit ...	2018	29237265
high incidence of citrus tristeza virus in mandarin (citrus reticulata) in north-east states of india.	khasi mandarin is one of the most remunerative crop in north eastern hills region of india playing a very critical role in the socio-economic upliftment of the people. the orchards of this region were found to express the typical symptoms of citrus tristeza virus (ctv). an extensive survey of the khasi mandarin orchard for six north eastern states of india namely arunachal pradesh, meghalaya, assam, nagaland, sikkim and tripura, was carried out to establish the identity of these viruses using el ...	2017	29291231
chemical constituents from the flowers of satsuma mandarin and their free radical scavenging and α-glucosidase inhibitory activities.	flowers of citrus plants are used as mild sedatives and for the treatment of insomnia in traditional medicines. in japan, tea made from the flowers of satsuma mandarin is consumed as healthy drink. hesperidin (1), hesperetin (2), rutin (3), quercetin (4), nicotiflorin (5), eriocitrin (6), narirutin (7), phenylethyl glucoside (8) and unshuoside a (9) were isolated from the meoh extract of fresh flowers. structure elucidation of these compounds was performed on the basis of nmr spectroscopic data. ...	2018	29334238
efficient analysis of phytochemical constituents in the peel of chinese wild citrus mangshanju (citrus reticulata blanco) by uhplc-q-tof-ms.	an efficient ultra high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry method was developed for separation and profiling of phytochemical constituents of chinese wild mandarin, mangshanju (citrus reticulata blanco). all constituents were well separated within 16 min. based on retention times, accurate mass, mse fragments and/or reference standards as well as databases, a total of 81 compounds were unambiguously identified or tentatively assigned includ ...	2018	29370467