Publications

TitleAbstractYear
Filter
PMID(sorted ascending)
Filter
an extracellular protease of pseudomonas fluorescens inactivates antibiotics of pantoea agglomerans.abstract pseudomonas fluorescens a506 and pantoea agglomerans strains eh252 and c9-1 are biological control agents that suppress fire blight, an important disease of pear and apple caused by the bacterium erwinia amylovora. pseudomonas fluorescens strain a506 suppresses disease largely through competitive exclusion of e. amylovora on surfaces of blossoms, the primary infection court, whereas pantoea agglomerans strains eh252 and c9-1 produce antibiotics that are toxic to e. amylovora. in this st ...200418944458
a rapid bioassay for screening rhizosphere microorganisms for their ability to induce systemic resistance.abstract we developed a rapid and miniaturized bioassay for screening large numbers of rhizosphere microorganisms for their ability to induce systemic resistance to bacterial leaf spot of radish caused by xanthomonas campestris pv. armoraciae. in this bioassay, pantoea agglomerans strain e278ar controlled symptoms of disease as effectively as 2,6-dichloroisonicotinic acid when applied to the roots of seedlings produced in growth pouches in a soilless system. e278ar essentially did not migrate fr ...200018944580
biological control of pseudomonas syringae pv. syringae, the causal agent of basal kernel blight of barley, by antagonistic pantoea agglomerans.abstract strains of pantoea agglomerans (synanamorph erwinia herbicola) suppressed the development of basal kernel blight of barley, caused by pseudomonas syringae pv. syringae, when applied to heads prior to the pseudomonas syringae pv. syringae infection window at the soft dough stage of kernel development. field experiments in 1994 and 1995 revealed 45 to 74% kernel blight disease reduction, whereas glasshouse studies resulted in 50 to 100% disease control depending on the isolate used and ba ...200018944586
effect of nectar on microbial antagonists evaluated for use in control of fire blight of pome fruits.abstract under warm, dry conditions, erwinia amylovora can become established in relatively high populations on apple (malus domestica) or pear (pyrus communis) flower stigmas, and subsequent wet conditions facilitate its movement to the flower hypanthium where infection generally is initiated through the nectarthodes. research on biological control of fire blight has focused mainly on the flower stigma, and knowledge is lacking regarding the effect of nectar on microbial antagonists in the flow ...199918944801
occurrence of indole-3-acetic acid-producing bacteria on pear trees and their association with fruit russet.abstract a relatively high percentage of epiphytic bacteria on pear leaf and fruit surfaces had the ability to produce indole-3-acetic acid (iaa) in culture media supplemented with tryptophan. while over 50% of the strains produced at least small amounts of iaa in culture, about 25% of the strains exhibited high iaa production as evidenced by both colorimetric and high-performance liquid chromatography analysis of culture supernatants. a majority of the strains that produced high amounts of iaa ...199818944847
compost and compost water extract-induced systemic acquired resistance in cucumber and arabidopsis.abstract a biocontrol agent-fortified compost mix, suppressive to several diseases caused by soilborne plant pathogens, induced systemic acquired resistance (sar) in cucumber against anthracnose caused by colletotrichum orbiculare and in arabidopsis against bacterial speck caused by pseudomonas syringae pv. maculicola kd4326. a peat mix conducive to soilborne diseases did not induce sar. the population size of p. syringae pv. maculicola kd4326 was significantly lower in leaves of arabidopsis pla ...199818944926
evaluation of sample preparation methods for the isolation of salmonella from alfalfa and mung bean seeds with the bacteriological analytical manual's salmonella culture method.five pre-enrichment methods were evaluated for effectiveness with the u.s. food and drug administration's bacteriological analytical manual salmonella culture method in recovering s. stanley, s. poona, and s. muenchen from artificially contaminated alfalfa seeds, and s. saintpaul, s. anatum, and s. infantis from artificially contaminated mung bean seeds. the methods included: (1) soak.--test portions were inoculated into pre-enrichment media; (2) rinse.--test portions were rinsed with pre-enrich ...200818980122
isolation of an operon involved in xylitol metabolism from a xylitol-utilizing pantoea ananatis mutant.an operon involved in cryptic xylitol metabolism of pantoea ananatis was cloned by transposon tagging. a xylitol negative mutant with a transposon insertion in the xylitol 4-dehydrogenase gene (xdh) was isolated and genomic dna around the transposon was sequenced. consequently, six consecutive genes, xytb-g are located downstream of xdh in the same strand. these seven genes are cotranscribed as a single transcript in a p. ananatis xylitol-utilizing mutant, suggesting that they comprise an operon ...200819000608
phylogeny and identification of pantoea species associated with plants, humans and the natural environment based on multilocus sequence analysis (mlsa).species belonging to the genus of pantoea are commonly isolated from plants, humans and the natural environment. the species of the genus are phenotypically closely related, making rapid identification of pantoea strains to the species level difficult. multilocus sequence analysis (mlsa) was evaluated as a means for rapid classification and identification of pantoea strains. four housekeeping genes, gyrb, rpob, atpd and infb, were sequenced for strains assigned to the genus. included in the stud ...200819008066
wtse, an avre-family type iii effector protein of pantoea stewartii subsp. stewartii, causes cell death in non-host plants.pantoea stewartii subsp. stewartii (pnss) causes stewart's bacterial wilt of sweet corn and leaf blight of maize. the pathogenicity of pnss depends on synthesis of extracellular polysaccharide and an hrp type iii secretion system. wtse, a type iii secreted effector protein, is essential for the virulence of pnss on corn. it belongs to the avre family of effectors, which includes dspa/e from erwinia amylovora and avre1 from pseudomonas syringae. previously, wtse was shown to cause disease-associa ...200819018993
phylogeny and identification of pantoea species and typing of pantoea agglomerans strains by multilocus gene sequencing.pantoea agglomerans and other pantoea species cause infections in humans and are also pathogenic to plants, but the diversity of pantoea strains and their possible association with hosts and disease remain poorly known, and identification of pantoea species is difficult. we characterized 36 pantoea strains, including 28 strains of diverse origins initially identified as p. agglomerans, by multilocus gene sequencing based on six protein-coding genes, by biochemical tests, and by antimicrobial sus ...200919052179
antimicrobial activity of pyrrocidines from acremonium zeae against endophytes and pathogens of maize.acremonium zeae produces pyrrocidines a and b, which are polyketide-amino acid-derived antibiotics, and is recognized as a seedborne protective endophyte of maize which augments host defenses against microbial pathogens causing seedling blights and stalk rots. pyrrocidine a displayed significant in vitro activity against aspergillus flavus and fusarium verticillioides in assays performed using conidia as inoculum, with pyrrocidine a being more active than b. in equivalent assays performed with c ...200919055442
study on the bacterial midgut microbiota associated to different brazilian populations of lutzomyia longipalpis (lutz & neiva) (diptera: psychodidae).the bacterial community associated with the midgut of three brazilian lutzomyia longipalpis (lutz & neiva) populations, two from endemic areas for visceral leishmaniasis (jacobina, bahia state and são luís, maranhão state) and one from a non-endemic area (lapinha cave, minas gerais state), was identified. five groups, 35 females each, from each population were separated; a total of 175 females per collecting area were analyzed. the species identification was based on molecular and traditional ba ...200819061048
characterization of the mineral phosphate-solubilizing activity of pantoea agglomerans mmb051 isolated from an iron-rich soil in southeastern venezuela (bolívar state).the mineral phosphate-solubilizing (mps) activity of a pantoea agglomerans strain, namely mmb051, isolated from an iron-rich, acidic soil near ciudad piar (bolívar state, venezuela), was characterized on a chemically defined medium (nbrip). various insoluble inorganic phosphates, including tri-calcium phosphate [ca(3)(po(4))(2)], iron phosphate (fepo(4)), aluminum phosphate (alpo(4)), and rock phosphate (rp) were tested as sole sources of p for bacterial growth. solubilization of ca(3)(po(4))(2) ...200919067045
dna analysis of a radiotolerant bacterium pantoea agglomerans by ft-ir spectroscopy.a radiation tolerance strain, pantoea agglomerans was isolated from gamma-irradiated carrot samples (daucus carota). d(10) determination showed that the radioresistance of this bacterium is five-fold higher than escherichia coli, both belonging to the family of enterobacteriaceae. dna isolated from untreated and irradiated bacterial cells was analyzed by ft-ir spectroscopy to investigate the radiotolerance of this bacterium. at doses <5kgy, an alteration of the interbase hydrogen networks was ob ...200719071550
complete genome of the broad-host-range erwinia amylovora phage phiea21-4 and its relationship to salmonella phage felix o1.the first complete genome sequence for a myoviridal bacteriophage, phiea21-4, infecting erwinia amylovora, erwinia pyrifoliae, and pantoea agglomerans strains has been determined. the unique sequence of this terminally redundant, circularly permuted genome is 84,576 bp. the phiea21-4 genome has a gc content of 43.8% and contains 117 putative protein-coding genes and 26 trna genes. phiea21-4 is the first phage in which a precisely conserved rho-independent terminator has been found dispersed thro ...200919181832
lines of evidence for horizontal gene transfer of a phenazine producing operon into multiple bacterial species.phenazines are secondary metabolites with broad-spectrum antibiotic activity against bacteria, fungi, and eukaryotes. in pseudomonad species, a conserved seven-gene phenazine operon (phzabcdefg) is required for the conversion of chorismic acid to the broad-spectrum antibiotic phenazine-1-carboxylate. previous analyses of genes involved in phenazine production from nonpseudomonad species uncovered a high degree of sequence similarity to pseudomonad homologues. the analyses undertaken in this stud ...200919189039
diverse antibacterial activity of pectobacterium carotovorum subsp.carotovorum isolated in korea.fifty-four pectobacterium carotovorum subsp. carotovorum strains isolated in korea were characterized by a spectrum of antibacterial activities against 7 indicator strains chosen to represent various regions and host plants. all p. carotovorum subsp. carotovorum isolates tested could be grouped into 4 classes depending on the pattern of antibacterial substance production. all tested strains had dna fragment(s) homologous to the genes encoding carotovoricin and 21 of them had genes homologous to ...200919190407
effect of gamma-irradiation on membrane fatty acids and peptidoglycan's muropeptides of pantoea agglomerans, a plant pathogen.the aim of this study was to evaluate the effect of gamma-irradiation on the fatty acids (fa) and muropeptides content of two strains of an enterobacteriacea: pantoea agglomerans.200919191968
pathway engineering of brassica napus seeds using multiple key enzyme genes involved in ketocarotenoid formation.brassica napus (canola) plants were genetically manipulated to increase the amount and composition of carotenoids in seeds by using seven key enzyme genes involved in ketocarotenoid formation, which originated from a soil bacterium pantoea ananatis (formerly called erwinia uredovora 20d3), and marine bacteria brevundimonas sp. strain sd212 and paracoccus sp. strain n81106 (formerly called agrobacterium aurantiacum). the seven key gene cassettes, in which each gene was surrounded by an appropriat ...200919204032
inoculation of sugarcane with pantoea sp. increases amino acid contents in shoot tissues; serine, alanine, glutamine and asparagine permit concomitantly ammonium excretion and nitrogenase activity of the bacterium.pantoea sp. is an endophytic nitrogen-fixing bacterium isolated from sugarcane tissues. the aim of the present study was to determine the contents of amino acids in sugarcane as a result of inoculation of nodes and nodal roots with pantoea sp. strain 9c and to evaluate the effects of amino acids on growth, nitrogenase activity and ammonium excretion of the bacterium. content of almost all amino acids increased in 30-day-old plantlets by root inoculation. the most abundant amino acids in shoot ti ...200919215998
study of microbial diversity in raw milk and fresh curd used for fontina cheese production by culture-independent methods.the bacterial populations of raw milk employed for the production of fontina cheese in alpine farms located in different valleys and altitudes (from 700 to 2246 m above sea level) were investigated by culture independent techniques. total microbial dna was isolated from milk and curd samples and used as template in polymerase chain reaction (pcr) to study the hypervariable v3 region of the bacterial 16s rrna gene and analyzed by denaturing gradient gel electrophoresis (dgge). representative band ...200919232767
temporal analysis of cotton boll symptoms resulting from southern green stink bug feeding and transmission of a bacterial pathogen.the southern green stink bug, nezara viridula (l.), is a significant pest of cotton, gossypium hirsutum l., and is becoming an increasing challenge due to the decrease in use of broad-spectrum insecticides on the crop. the southern green stink bug can vector an opportunistic pantoea agglomerans strain (designated sc 1-r) into cotton bolls, resulting in infection. the appearance of stink bug damage varies, and pest managers cannot readily identify its source. this research reports a systematic de ...200919253615
effect of mutagenesis at the region upstream from the g(q/e) motif of three types of geranylgeranyl diphosphate synthase on product chain-length.(all-e) geranylgeranyl diphosphate synthases have been classified into three types based on the characteristic sequences around the first aspartate rich motif, which is highly conserved among the enzymes. in type i geranylgeranyl diphosphate synthases, which consist of archaeal enzymes, a bulky amino acid residue at the 5th position upstream from the motif plays a main role in the product determination, by blocking further elongation of prenyl chain as the bottom of the reaction cavity. on the o ...200919269584
adverse effects of pantoea peritonitis on peritoneal transport. 200919293363
epiphytic bacteria and yeasts on apple blossoms and their potential as antagonists of erwinia amylovora.apple blossoms were sampled for indigenous epiphytic populations of culturable microorganisms during different stages of bloom at two locations in central washington state and one site in corvallis, or. frequencies and population sizes of bacteria on stigmas of apple were lower in washington than at corvallis, where average relative humidity was higher and possibly favored greater colonization; however, bacteria at corvallis were mainly pseudomonads, whereas those in washington were diverse, com ...200919351253
traumatic enucleation with avulsion of the ophthalmic artery resulting in aneurysm-like subarachnoid hemorrhage.traumatic, nonaneurysmal subarachnoid hemorrhage (sah) is common after closed head injury and most often results from ruptured cortical microvessels. here, the authors present the case of a 60-year-old woman who fell and struck her head, causing traumatic enucleation and avulsion of both the optic nerve and ophthalmic artery. the arterial avulsion caused a fisher grade 3 sah. during her stay in the intensive care unit, hydrocephalus and vasospasm developed, clinical conditions commonly observed ...200919374504
six cases of sepsis caused by pantoea agglomerans in a teaching hospital.pantoea agglomerans is an environmental organism which may seldom cause opportunistic infections. here we report on a 6 case outbreak in a teaching hospital. within three months . agglomerans was isolated from blood cultures of 5 patients from oncology and 1 patient from icu departments. p. agglomerans was in pure culture in 5 cases, while in the last one rahnella aquatilis and candida famata were also isolated. therefore, p. agglomerans is able to produce nosocomial infections in patients with ...200919382678
use of the lambda red-recombineering method for genetic engineering of pantoea ananatis.pantoea ananatis, a member of the enterobacteriacea family, is a new and promising subject for biotechnological research. over recent years, impressive progress in its application to l-glutamate production has been achieved. nevertheless, genetic and biotechnological studies of pantoea ananatis have been impeded because of the absence of genetic tools for rapid construction of direct mutations in this bacterium. the lambda red-recombineering technique previously developed in e. coli and used for ...200919389224
recent evolution of bacterial pathogens: the gall-forming pantoea agglomerans case.pantoea agglomerans, a widespread epiphyte and commensal bacterium, has evolved into an hrp-dependent and host-specific tumorigenic pathogen by acquiring a plasmid containing a pathogenicity island (pai). the pai was evolved on an iteron plasmid of the incn family, which is distributed among genetically diverse populations of p. agglomerans. the structure of the pai supports the premise of a recently evolved pathogen. this review offers insight into a unique model for emergence of new bacterial ...200919400643
pantoea ananatis: an unconventional plant pathogen.pantoea ananatis causes disease symptoms in a wide range of economically important agricultural crops and forest tree species worldwide. it is regarded as an emerging pathogen based on the increasing number of reports of diseases occurring on previously unrecorded hosts in different parts of the world. its unconventional nature lies in the fact that, unlike the majority of plant pathogenic microbes, p. ananatis is capable of infecting humans and occurs in diverse ecological niches, such as part ...200919400836
isolation and characterization of plant growth-promoting strain pantoea nii-186. from western ghat forest soil, india.to isolate plant growth-promoting bacterium from western ghat forests in india.200919413772
utility and safety of lps-based fermented flour extract as a macrophage activator.the immune system is part of the homeostasis system. our research is focused on ways to maintain health, with an emphasis on the role of macrophages. we have hypothesized that tissue macrophages form a systemic network which we believe contributes to the homeostasis system, and have named it the 'macrophage network.' this network creates a dynamic equilibrium situation where macrophages control homeostasis. our research is based on this macrophage network theory as we believe that the innate imm ...200919414320
blood culture status in mature horses with diarrhoea: a possible association with survival.the incidence and implications of positive blood cultures in mature horses with diarrhoea is unknown. the diagnosis of bacteraemia may alter treatment and prognosis.200919418745
multiple activities of the plant pathogen type iii effector proteins wtse and avre require wxxxe motifs.the broadly conserved avre-family of type iii effectors from gram-negative plant-pathogenic bacteria includes important virulence factors, yet little is known about the mechanisms by which these effectors function inside plant cells to promote disease. we have identified two conserved motifs in avre-family effectors: a wxxxe motif and a putative c-terminal endoplasmic reticulum membrane retention/retrieval signal (ermrs). the wxxxe and ermrs motifs are both required for the virulence activities ...200919445595
bacterial associates of arboreal ants and their putative functions in an obligate ant-plant mutualism.bacterial communities are highly diverse and have great ecological importance. in the present study, we used an in silico analysis of terminal restriction fragments (trf) to characterize the bacterial community of the plant ant pseudomyrmex ferrugineus. this species is an obligate inhabitant of acacia myrmecophytes and feeds exclusively on plant-derived food sources. ants are the dominant insect group in tropical rain forests. associations of ants with microbes, which contribute particularly to ...200919447959
isolation and characterization of rhamnolipid-producing bacterial strains from a biodiesel facility.novel strains of rhamnolipid-producing bacteria were isolated from soils at a biodiesel facility on the basis of their ability to grow on glycerol as a sole carbon source. strains were identified as acinetobacter calcoaceticus, enterobacter asburiae, enterobacter hormaechei, pantoea stewartii, and pseudomonas aeruginosa. the strains of the former five species were found to produce rhamnolipids in quantities the same as, or similar to, coisolated strains of p. aeruginosa. measurements of surface ...200919473254
antibiotic resistance of gram negatives isolates from loggerhead sea turtles (caretta caretta) in the central mediterranean sea.previous studies on fish and marine mammals support the hypothesis that marine species harbor antibiotic resistance and therefore may serve as reservoirs for antibiotic-resistance genetic determinants. the aim of this study was to assess the resistance to antimicrobial agents of gram negative strains isolated from loggerhead sea turtles (carettacaretta). oral and cloacal swabs from 19 live-stranded loggerhead sea turtles, with hooks fixed into the gut, were analyzed. the antimicrobial resistance ...200919473669
comparative genomics-guided loop-mediated isothermal amplification for characterization of pseudomonas syringae pv. phaseolicola.to design and evaluate a loop-mediated isothermal amplification (lamp) protocol by combining comparative genomics and bioinformatics for characterization of pseudomonas syringae pv. phaseolicola (psp), the causal agent of halo blight disease of bean (phaseolus vulgaris l.).200919486391
phenotypic and genetic diversity of rice seed-associated bacteria and their role in pathogenicity and biological control.to study the phenotypic and genetic diversity of culturable bacteria associated with rice seed and to asses the antagonistic and pathogenic potential of the isolated bacteria.200919486410
the microbiota of lafun, an african traditional cassava food product.lafun is a fermented cassava food product consumed in parts of west africa. in the present work the microorganisms (aerobic bacteria (ab), lactic acid bacteria (lab) and yeasts) associated with the fermentation of lafun under traditional conditions have for the first time been studied using a combination of pheno- and genotypic methods. during lafun fermentation the ab count ranged from 6-7 log(10) cfu/g at the beginning to 9 log(10) cfu/g at the end. similarly, the number of lab increased from ...200919493582
regulatory interactions between quorum-sensing, auxin, cytokinin, and the hrp regulon in relation to gall formation and epiphytic fitness of pantoea agglomerans pv. gypsophilae.gall formation by pantoea agglomerans pv. gypsophilae is controlled by hrp/hrc genes, phytohormones, and the quorum-sensing (qs) regulatory system. the interactions between these three components were investigated. disruption of the qs genes pagi and pagr and deletion of both substantially reduced the transcription levels of the hrp regulatory genes hrpxy, hrps, and hrpl, as determined by quantitative reverse-transcriptase polymerase chain reaction. expression of hrpl in planta was inhibited by ...200919522567
bacterial community and nitrogen fixation in the red turpentine beetle, dendroctonus valens leconte (coleoptera: curculionidae: scolytinae).the red turpentine beetle, dendroctonus valens leconte (coleoptera: curculionidae: scolytinae), colonizes all pines species within its native range throughout north and central america. recently, this species was accidentally introduced to china, where it has caused severe damage in pine forests. it belongs to a group of beetles that spend most of their lives between the tree bark and sapwood, where it feeds on phloem: a poor substrate with very low nutritional value of nitrogen and toxic proper ...200919543937
arsenic detoxification potential of aox genes in arsenite-oxidizing bacteria isolated from natural and constructed wetlands in the republic of korea.arsenic is subject to microbial interactions, which support a wide range of biogeochemical transformations of elements in natural environments such as wetlands. the arsenic detoxification potential of the bacterial strains was investigated with the arsenite oxidation gene, aox genotype, which were isolated from the natural and constructed wetlands. the isolates were able to grow in the presence of 10 mm of sodium arsenite (as(iii) as naaso(2)) and 1 mm of d: +glucose. phylogenetic analysis based ...201019548094
carotenoids' influence on radiotolerance of pantoea agglomerans, a plant pathogen.the aim of this study was to evaluate the effect of gamma radiation on the carotenoid content of two strains of the enterobacteriaceae: pantoea agglomerans.200819552787
broad-range pcr, cloning and sequencing of the full 16s rrna gene for detection of bacterial dna in synovial fluid samples of tunisian patients with reactive and undifferentiated arthritis.broad-range rdna pcr provides an alternative, cultivation-independent approach for identifying bacterial dna in reactive and other form of arthritis. the aim of this study was to use broad-range rdna pcr targeting the 16s rrna gene in patients with reactive and other forms of arthritis and to screen for the presence of dna from any given bacterial species in synovial fluid (sf) samples.200919570210
occupational exposure to organic dust, microorganisms, endotoxin and peptidoglycan among plants processing workers in poland.the objective of present work was to determine and compare the components of bioaerosol in several sectors of plant processing industries. the study was conducted in 10 facilities engaged in herb and grain processing, flax threshing, grain storing, baking, and cereals production. the air samples were taken on glass fibre filters with an as-50 sampler. we determined the concentrations of airborne microorganisms, dust, endotoxin and peptidoglycan. total concentrations of viable airborne microorgan ...200919572487
a novel inhalation challenge set to study animal model of allergic alveolitis.a novel inhalation challenge set for the study of experimental allergic alveolitis (hypersensitivity pneumonitis) in mice was designed. a finely dispersed aerosol of allergenic extract generated by the commercial ultrasonic nebulizer "tajfun mu1" (produced by medbryt, warsaw, poland) was transported to the airtight inhalation chamber. in the chamber were placed 15 perforated containers made of transparent plastic, each containing one mouse. they were coupled in 3 units, each consisted of 5 conta ...200919572490
evaluation of two spike-and-recovery controls for assessment of extraction efficiency in microbial source tracking studies.quantitative pcr (qpcr), applied to complex environmental samples such as water, wastewater, and feces, is susceptible to methodological and sample related biases. in this study, we evaluated two exogenous dna spike-and-recovery controls as proxies for recovery efficiency of bacteroidales 16s rdna gene sequences (allbac and qhf183) that are used for microbial source tracking (mst) in river water. two controls--(1) the plant pathogen pantoea stewartii, carrying the chromosomal target gene cpsd, a ...200919589555
an assessment of bacterial dysbiosis in pouchitis using terminal restriction fragment length polymorphisms of 16s ribosomal dna from pouch effluent microbiota.previous studies on dysbiosis and pouchitis using conventional culture techniques have been disappointing because of inherent limitations associated with the technique. this study was designed to use terminal restriction fragment length polymorphism to evaluate patients with and without pouchitis.200919617766
pantoea vagans sp. nov., pantoea eucalypti sp. nov., pantoea deleyi sp. nov. and pantoea anthophila sp. nov.bacteria isolated from eucalyptus leaves and shoots showing symptoms of blight and die-back collected in uganda, uruguay and argentina and from maize displaying brown stalk rot symptoms in south africa were tentatively placed in the genus pantoea on the basis of phenotypic and biochemical tests. these isolates, together with two strains (lmg 2558 and lmg 2560) previously assigned to pantoea agglomerans based on protein electrophoregrams but later excluded from this species, were further investig ...200919620357
altered fecal microflora and increased fecal calprotectin in infants with colic.we explored whether gut inflammation, colonic fermentation, and/or an altered colonic flora could provide a pathophysiological mechanism for colic.200919628216
potential for nezara viridula (hemiptera: pentatomidae) to transmit bacterial and fungal pathogens into cotton bolls.recently, we showed that the southern green stink bug (sgsb), nezara viridula (l.), can transmit pantoea agglomerans (ewing and fife), an opportunistic bacterium, into green cotton bolls resulting in plant disease. here, we hypothesized that our established model could be used to determine if the sgsb was a general, non-discriminate vector by using two other opportunistic bacterial pathogens of bolls (pantoea ananatis [serano] and klebsiella pneumoniae [schroeter]) and the known fungal pathogen ...200919636620
pantoea agglomerans: the gardener's peritonitis? 200919640377
phylogenetic evidence for extensive horizontal gene transfer of type iii secretion system genes among enterobacterial plant pathogens.this study uses sequences from four genes, which are involved in the formation of the type iii secretion apparatus, to determine the role of horizontal gene transfer in the evolution of virulence genes for the enterobacterial plant pathogens. sequences of erwinia, brenneria, pectobacterium, dickeya and pantoea were compared (a) with one another, (b) with sequences of enterobacterial animal pathogens, and (c) with sequences of plant pathogenic gamma and beta proteobacteria, to evaluate probable p ...200919643761
transfer of pantoea citrea, pantoea punctata and pantoea terrea to the genus tatumella emend. as tatumella citrea comb. nov., tatumella punctata comb. nov. and tatumella terrea comb. nov. and description of tatumella morbirosei sp. nov.pantoea citrea, pantoea punctata and pantoea terrea were described for strains isolated from fruit and soil originating in japan. these three 'japanese' species have been shown to be phylogenetically distant from other species of the genus pantoea. it has been observed previously that, using multilocus sequence analysis (mlsa), the 'japanese' species consistently formed a distinct clade with an extended branch length, casting doubt on the inclusion of these species within the genus pantoea. furt ...201019654354
population dynamics and identification of endophytic bacteria antagonistic toward plant-pathogenic fungi in cotton root.the antagonistic potentials of endophytic bacteria isolated from the roots of six cotton cultivars at different developmental stages were determined in vitro toward three pathogens: verticillium dahliae kleb v107 and v396 and fusarium oxysporum f.sp. vasinfectum (f108). the populations of antagonistic endophytic bacteria (aeb) toward v107, v396, and f108 at the flowering and maturation stages were significantly higher than those at the seedling stage were. more aeb were found to be antagonistic ...201019669227
isolation of enterobacter cowanii from eucalyptus showing symptoms of bacterial blight and dieback in uruguay.this study was performed to identify bacterial strains isolated simultaneously with pantoea species from eucalyptus trees showing symptoms of bacterial blight and dieback in uruguay.200919674289
the potential anti-herbivory role of microorganisms on plant thorns.thorns, spines and prickles are some of the anti-herbivore defenses that plants have evolved. they were recently found to be commonly aposematic (warning coloration). however, the physical anti-herbivore defense executed by these sharp structures seems to be only the tip of the iceberg. we show that thorns of various plant species commonly harbor an array of aerobic and anaerobic pathogenic bacteria including clostridium perfringens the causative agent of the life-threatening gas gangrene, bacil ...200719704540
prevalence of ewingella americana in retail fresh cultivated mushrooms (agaricus bisporus, lentinula edodes and pleurotus ostreatus) in zaragoza (spain).the prevalence and mycopathogenic potential of enterobacteriaceae (especially ewingella americana) in cultivated mushrooms were studied. a total of 95 samples of agaricus bisporus, lentinula edodes and pleurotus ostreatus were analyzed to quantify the enterobacteriaceae and to identify the species isolated. the host pathogenicity test was used to verify their mycopathogenic potential. the genus pseudomonas was also quantified, since it is the predominant bacterial group in cultivated mushrooms. ...200419712317
comparison of the bacterial community and characterization of plant growth-promoting rhizobacteria from different genotypes of chrysopogon zizanioides (l.) roberty (vetiver) rhizospheres.molecular approaches [pcr-denaturing gradient gel electrophoresis (dgge)] were used to determine whether three different vetiver (chrysopogon zizanioides) genotypes, commercially used in brazil and considered economically important over the world, select specific bacterial populations to coexist in their rhizospheres. dgge profiles revealed that the predominant rhizospheric bacterial community hardly varies regarding the vetiver genotype. moreover, using traditional cultivation methods, bacteria ...200919763409
endophytic bacterial diversity in grapevine (vitis vinifera l.) leaves described by 16s rrna gene sequence analysis and length heterogeneity-pcr.diversity of bacterial endophytes associated with grapevine leaf tissues was analyzed by cultivation and cultivation-independent methods. in order to identify bacterial endophytes directly from metagenome, a protocol for bacteria enrichment and dna extraction was optimized. sequence analysis of 16s rrna gene libraries underscored five diverse operational taxonomic units (otus), showing best sequence matches with gamma-proteobacteria, family enterobacteriaceae, with a dominance of the genus panto ...200919763412
genotypic comparison of pantoea agglomerans plant and clinical strains.pantoea agglomerans strains are among the most promising biocontrol agents for a variety of bacterial and fungal plant diseases, particularly fire blight of apple and pear. however, commercial registration of p. agglomerans biocontrol products is hampered because this species is currently listed as a biosafety level 2 (bl2) organism due to clinical reports as an opportunistic human pathogen. this study compares plant-origin and clinical strains in a search for discriminating genotypic/phenotypic ...200919772624
characterization of a broad-host-range flagellum-dependent phage that mediates high-efficiency generalized transduction in, and between, serratia and pantoea.a phage (phiot8) isolated on serratia sp. atcc 39006 was shown to be flagellum-dependent, and to mediate generalized transduction with high efficiency (up to 10(-4) transductants per p.f.u.). phiot8 was shown to have a broad host range because it also infected a strain of pantoea agglomerans isolated from the rhizosphere. transduction of plasmid-borne antibiotic resistance between the two bacterial genera was demonstrated, consistent with purported ecological roles of phages in dissemination of ...201019778959
a single genetic locus in the phytopathogen pantoea stewartii enables gut colonization and pathogenicity in an insect host.aphids are typically exposed to a variety of epiphytic and phytopathogenic bacteria, many of which have entomopathogenic potential. here we describe the interaction between pantoea stewartii ssp. stewartii dc283 (dc283), an enteric phytopathogen and causal agent of stewart's wilt, and the pea aphid, acyrthosiphon pisum. when ingested by aphids, dc283 establishes and aggregates in the crop and gut, preventing honeydew flow and excretion, resulting in aphid death in 72 h. a mutagenesis screen iden ...201019788413
association and attraction of blueberry maggot fly curran (diptera: tephritidae) to pantoea (enterobacter) agglomerans.the attraction of washed, medium-free cells of pantoea (enterobacter) agglomerans to wild, adult rhagoletis mendax curran, the blueberry maggot fly, was evaluated in managed blueberry fields in maine. attraction was evaluated using pherocon am and ladd traps, each tested with or without washed bacterial cells. field studies showed significant increases in fly captures on the pherocon am traps. apple volatiles odors on ladd traps seemed to cancel the effects of bacterial odors. aerobic heterotrop ...200919791604
polyphasic study of plant- and clinic-associated pantoea agglomerans strains reveals indistinguishable virulence potential.pantoea species are ubiquitous in nature and occasionally associated with infections caused by contaminated clinical material. hence, pantoea agglomerans is considered as an opportunistic pathogen of humans. since species of the genus pantoea and closely related species of other enterobacteriaceae genera are phenotypically very similar, many clinical isolates are misassigned into p. agglomerans based on the use of quick commercial-offered biochemical tests. our objective was to find markers enab ...200919800991
the atp-dependent amide ligases ddag and ddaf assemble the fumaramoyl-dipeptide scaffold of the dapdiamide antibiotics.the enzymes ddag and ddaf, encoded in the pantoea agglomerans dapdiamide antibiotic biosynthetic gene cluster, when expressed in escherichia coli, form the tandem amide bonds of the dapdiamide scaffold at the expense of atp cleavage. ddag uses fumarate, 2,3-diaminopropionate (dap), and atp to make fumaroyl-amp transiently on the way to the n(beta)-fumaroyl-dap regioisomer. then ddaf acts as a second atp-dependent amide ligase, but this enzyme cleaves atp to adp and p(i) during amide bond formati ...200919807062
structure/function analysis of the pantoea stewartii quorum-sensing regulator esar as an activator of transcription.in pantoea stewartii subsp. stewartii, two regulatory proteins are key to the process of cell-cell communication known as quorum sensing: the luxi and luxr homologues esai and esar. most luxr homologues function as activators of transcription in the presence of their cognate acylated homoserine lactone (ahl) signal. however, esar was initially found to function as a repressor in the absence of ahl. previous studies demonstrated that, in the absence of ahl, esar retains the ability to function as ...200919820098
2-amino-3-(oxirane-2,3-dicarboxamido)-propanoyl-valine, an effective peptide antibiotic from the epiphyte pantoea agglomerans 48b/90.the epiphyte pantoea agglomerans 48b/90, which has been isolated from soybean leaves, belongs to the enterobacteriaceae, as does the plant pathogen erwinia amylovora, which causes fire blight on rosaceous plants such as apples and leads to severe economic losses. since p. agglomerans efficiently antagonizes phytopathogenic bacteria, the p. agglomerans strain c9-1 is used as a biocontrol agent (blightban c9-1). here we describe the bioassay-guided isolation of a peptide antibiotic that is highly ...200919820144
microbial diversity changes in soybean sprouts treated with enterocin as-48.seed sprouts may act as vehicles for foodborne pathogenic bacteria. in the present study, the effect of washing treatment with the enterococcal bacteriocin enterocin as-48 on the microbiota of two batches of soybean sprouts was studied by culture-dependent and independent methods throughout storage at 10 degrees c. viable cell counts of bacteriocin-treated samples revealed some modifications only for lactic acid bacteria and enterococci during storage. in the control samples from batch 1, the cu ...200919835782
identification and characterization of three novel esai/esar quorum-sensing controlled stewartan exopolysaccharide biosynthetic genes in pantoea stewartii ssp. stewartii. 200919843225
[genetic diversity of cultivable bacteria of dominant plants in a potassium mine tailing of nanjing].plant rhizosphere bacteria play an important role in biogeochemical cycles. microbial diversity of cultivable bacteria from soil-plant-mineral system in a mine tailing was assessed.200919873749
uranium(vi) reduction and removal by high performing purified anaerobic cultures from mine soil.biological uranium reduction was investigated using bacteria isolated from a uranium mine in limpopo, south africa. background uranium concentration in soil from the mine was determined to be 168 mgkg(-1) much higher than the typical background uranium concentration in natural soils (0.30-11.7 mgkg(-1)). therefore it was expected that the bacteria isolated from the site were resistant to u(vi) toxicity. preliminary studies using a non-purified consortium from the mine soil showed that u(vi) [ura ...201019883933
micro-scale water potential gradients visualized in soil around plant root tips using microbiosensors.water availability and movement in soil are critical determinants of resource availability to, and interactions among, members of the soil community. however, it has been impossible to observe gradients in soil water potential empirically at millimetre spatial scales. here we describe progress towards that goal using output from two microbial biosensors, pantoea agglomerans brt98/ppprogreen and pseudomonas putida kt2442/ppprogreen, engineered with a reporter system based on the osmotically sensi ...201019906152
technology-induced selection towards the spoilage microbiota of artisan-type cooked ham packed under modified atmosphere.the microbiota associated with a highly-perishable belgian artisan-type cooked ham was analyzed through plating and (gtg)(5)-fingerprinting of isolates throughout its processing chain. the raw tumbled meat was characterized by the presence of a versatile microbiota around 4.8 log(cfu g(-1)), consisting of lactic acid bacteria, staphylococci, brochothrix thermosphacta, gram-negative bacteria, and yeasts. pasteurisation of the ham logs reduced bacterial counts below 2 log(cfu g(-1)) and subsequent ...201019913696
bacteria colonizing root nodules of wild legumes exhibit virulence-associated properties of mammalian pathogens.bacteria not proficient in nitrogen fixing symbiosis were proven able to invade root nodules of three wild legumes of the genus hedysarum in algeria and to be multiplying in these in place of the natural rhizobium symbionts. the involved species featured taxa known as human pathogens including: enterobacter cloacae, enterobacter kobei, escherichia vulneris, pantoea agglomerans and leclercia adecarboxylata. a direct screening of the phenotypic determinants of virulence using human cultured cells ...201019916054
robustness of the bacterial community in the cabbage white butterfly larval midgut.microbial communities typically vary in composition and structure over space and time. little is known about the inherent characteristics of communities that govern various drivers of these changes, such as random variation, changes in response to perturbation, or susceptibility to invasion. in this study, we use 16s ribosomal rna gene sequences to describe variation among bacterial communities in the midguts of cabbage white butterfly (pieris rapae) larvae and examine the influence of community ...201019924467
[bacterial diversity recovered from qiyi glacier and runoff, qilian mts].abundance and species diversity of bacteria were investigated respectively by epifluorescence microscope, the culture method and the analysis of 16s rdna genes, with snow and runoff samples from qiyi glacier in the qilian mts. according to the results, the total microbial cells and bacterial cfu range from 10(3) to 10(5) cells x ml(-1) and 0-600 cfu x ml(-1), respectively. the 16s rdna gene of 22 bacterial isolates recovered from snow and runoff samples belong to following groups: bacteroidetes, ...200919927833
characterization of an endophytic bacterial community associated with eucalyptus spp.endophytic bacteria were isolated from stems of eucalyptus spp (eucalyptus citriodora, e. grandis, e. urophylla, e. camaldulensis, e. torelliana, e. pellita, and a hybrid of e. grandis and e. urophylla) cultivated at two sites; they were characterized by rapd and amplified rdna restriction analysis (ardra). endophytic bacteria were more frequently isolated from e. grandis and e. pellita. the 76 isolates were identified by 16s rdna sequencing as erwinia/pantoea (45%), agrobacterium sp (21%), curt ...200919937585
[phytopathogenic bacteria of couch-grass in the crops of wheat].bacterialdiseases of weeds in the crops of wheat on the fields of kyiv and vinnytsya regions of ukraine elytrigia repens (l.) nevski agropyrum repens l. were revealed. the following symptoms of bacterial affections: the leaves wither, oval or hatched necrotic spots on green leaves, necroses on the stalks, empty-ears, partial blackening of the ear axes, awns, caryopsises, scales, water-soaked or dark brown with violet shade spots on the rhizomes were found. during the vegetation period bacteria w ...200919938603
a first report on the microbial colonisation of the equine oesophagus.based on cryo-sem, standard and high resolution tem, glycoconjugate histochemistry, and microbiological differentiation, the present study demonstrates the colonisation of the epithelium of the equine oesophagus with microorganisms. as particularly apparent using cryo-sem to illustrate natural conditions, the present microbiota were clearly dominated by bacteria, forming a one-layer system, as attached to and embedded in concentrated mannose/mannan substances covering the outer stratum corneal c ...201019942420
emended description of the genus pantoea, description of four species from human clinical samples, pantoea septica sp. nov., pantoea eucrina sp. nov., pantoea brenneri sp. nov. and pantoea conspicua sp. nov., and transfer of pectobacterium cypripedii (hori 1911) brenner et al. 1973 emend. hauben et al. 1998 to the genus as pantoea cypripedii comb. nov.bacterial strains belonging to dna hybridization groups (hg) ii, iv and v, in the erwinia herbicola-enterobacter agglomerans complex, of brenner et al. [int j syst bacteriol 34 (1984), 45-55] were suggested previously to belong to the genus pantoea, but have never been formally described and classified. additionally, it has been shown in several studies that pectobacterium cypripedii is more closely related to species of pantoea than to those of pectobacterium. in this study, the phylogenetic po ...201019946052
a simple and reliable method to conduct and monitor expression cassette integration into the escherichia coli chromosome.we report a method for the integration of expression cassettes into the escherichia coli chromosome using rare and dispensable sugar degradation gene loci as sites for integration. clones carrying successfully recombined dna fragments in the chromosome are easily screened using a solid differential medium containing the respective sugar compound. as an example for the heterologous expression of a complex natural product biosynthesis pathway, we show the stepwise chromosomal integration of the ze ...201019946879
retained hawthorn fragment in a child's foot complicated by infection: diagnosis and excision aided by localization with ultrasound.puncture wounds in children are very common and often result in foreign body retention. organic materials in the form of plant thorns present problems in identification and localization because they are not visualized with plain radiographs. a case of a 10-year-old girl with a small piece of retained hawthorn is presented. correct diagnosis and treatment were delayed because of misinterpretation of magnetic resonance image studies. ultrasound ultimately located the foreign body, and assisted in ...201019962326
symbiotic nitrogen fixation in the fungus gardens of leaf-cutter ants.bacteria-mediated acquisition of atmospheric n2 serves as a critical source of nitrogen in terrestrial ecosystems. here we reveal that symbiotic nitrogen fixation facilitates the cultivation of specialized fungal crops by leaf-cutter ants. by using acetylene reduction and stable isotope experiments, we demonstrated that n2 fixation occurred in the fungus gardens of eight leaf-cutter ant species and, further, that this fixed nitrogen was incorporated into ant biomass. symbiotic n2-fixing bacteria ...200919965433
discrete site surface complexation constants for lanthanide adsorption to bacteria as determined by experiments and linear free energy relationships.bacteria are abundant in many natural and engineered environments where they are thought to exert important controls on the cycling, mobility, bioavailability, and toxicity of metal contaminants. in order to probe their role in moderating the behavior of lanthanides, ph-dependent adsorption edges of 13 individual lanthanides and yttrium to the gram-negative bacterium pantoea agglomerans were used to generate discrete site surface complexation constants. the calculated surface complexation consta ...201020000843
the purb gene controls rhizosphere colonization by pantoea agglomerans.to isolate the rhizosphere competence-defective transposon tn5 mutant of pantoea agglomerans nbrisrm (srm) and to identify the gene causing defect in its root colonization ability.201020002573
miniprimer pcr assay targeting multiple genes: a new rapid and reliable tool for genotyping pantoea stewartii subsp. stewartii.development of a 'miniprimer' pcr assay for genotyping pantoea stewartii subsp. stewartii, the causal agent of the stewart's bacterial wilt on maize.201020002575
use of a foam spatula for sampling surfaces after bioaerosol deposition.the present study had three goals: (i) to evaluate the relative quantities of aerosolized bacillus atrophaeus spores deposited on the vertical, horizontal top, and horizontal bottom surfaces in a chamber; (ii) to assess the relative recoveries of the aerosolized spores from glass and stainless steel surfaces with a polyester swab and a macrofoam sponge wipe; and (iii) to estimate the relative recovery efficiencies of aerosolized b. atrophaeus spores and pantoea agglomerans using a foam spatula a ...201020023101
[population dynamics and antagonism toward fusarium oxysporium f. sp. vasinfectum. and verticillium dahliae kleb of endophytic bacteria from cotton].to explore population dynamics of endophytic bacteria and obtain antagonistic endophytic bacteria toward verticillium dahliae kleb (vd), fusarium oxysporium f. sp. vasinfectum (fov) from cotton.200920030058
improvement of allergic dermatitis via regulation of the th1/th2 immune system balance by macrophages activated with lipopolysaccharide derived from pantoea agglomerans (ip-pa1).recently, the incidence of allergies has been increasing, especially in advanced countries. the cause of these allergies is believed to be a failure in the immune system balance that has been caused by changes in the living environment. the incidence of allergy shows a negative correlation with the decrease of infectious diseases in childhood. it has been suggested that the key to alleviating allergies is to activate innate immunity by exposure to microbial components such as lipopolysaccharides ...200920032449
comparative immunological analysis of innate immunity activation after oral administration of wheat fermented extract to teleost fish.intradermal or oral administration of lipopolysaccharide derived from pantoea agglomerans (ip-pa1) has shown prophylactic and antitumor effects without serious side-effects. while it is known that tumor necrosis factor (tnf)-alpha produced by activated macrophages plays an important role in the expression mechanism following intradermal administration, details of the mechanism after oral administration remain unclear. in this study, the activation of innate immunity using fish as an animal model ...200920032450
immune recovery effects of immunopotentiator from pantoea agglomerans 1 (ip-pa1) on low antibody productions in response to salmonella enteritidis vaccine and sheep red blood cells in dexamethasone-treated stressed chicken models.considering the usefulness of the immunopotentiator from pantoea agglomerans 1 (ip-pa1), which is a purified lipopolysaccharide (lps) derived from symbiotic gram-negative bacteria of food crops, in controlling immunosuppression in poultry husbandry, in this study, we examined its immune-recovery effects in dexamethasone-treated stressed chicken models. three-week-old chickens daily administered 10 microg/kg of dexamethasone for 35 days to induce stress showed more whole body weight loss; relativ ...201020035113
dapdiamides, tripeptide antibiotics formed by unconventional amide ligases.construction of a genomic dna library from pantoea agglomerans strain cu0119 and screening against the plant pathogen erwinia amylovora yielded a new family of antibiotics, dapdiamides a-e (1-5). the structures were established through 2d-nmr experiments and mass spectrometry, as well as the synthesis of dapdiamide a (1). transposon mutagenesis of the active cosmid allowed identification of the biosynthetic gene cluster. the dapdiamide family's promiscuous biosynthetic pathway contains two uncon ...201020041689
pantoea gaviniae sp. nov. and pantoea calida sp. nov., isolated from infant formula and an infant formula production environment.five gram-negative, facultatively anaerobic, non-spore-forming, coccoid rod-shaped bacterial isolates were obtained from infant formula and an infant formula production environment and were investigated by use of a polyphasic taxonomic study. biochemical tests and partial rpob gene sequence analysis of the five isolates revealed that they formed two distinct groups in the family enterobacteriaceae, closely related to several species of the genera pantoea and erwinia, which indicated a phylogenet ...201020061487
molecular and biochemical characterization of three bacterial symbionts of fruit fly, bactrocera tau (tephritidae: diptera).characterization of three bacterial symbionts (bc1, bc2 and bc3) of fruit fly bactrocera tau including morphological, biochemical and 16s rdna (rrs gene) analysis was done to determine their taxonomic position. morphological and biochemical characterization placed two bacteria (bc1, klebsiella oxytoca and bc2, pantoea agglomerans) into family enterobacteriaceae and the third one (bc3, staphylococcus sp.) into family staphylococcaceae. 16s rdna gene sequence comparison with the available ncbi dat ...200920118612
histamine, cadaverine, and putrescine produced in vitro by enterobacteriaceae and pseudomonadaceae isolated from spinach.a total of 364 bacterial isolates, obtained from spinach leaves, were assayed in a decarboxylase broth containing histidine, lysine, and ornithine to check their ability to produce biogenic amines, and then quantified by high-performance liquid chromatography. among these isolates, 240 formed cadaverine, 208 formed putrescine, and 196 formed histamine, in widely varying amounts. they frequently produced more than one biogenic amine. klebsiella pneumoniae subsp. pneumoniae and morganella morganii ...201020132689
the lycopene cyclase crty from pantoea ananatis (formerly erwinia uredovora) catalyzes an fadred-dependent non-redox reaction.the cyclization of lycopene generates provitamin a carotenoids such as beta-carotene and paves the way toward the formation of cyclic xanthophylls playing distinct roles in photosynthesis and as precursors for regulatory molecules in plants and animals. the biochemistry of lycopene cyclization has been enigmatic, as the previously proposed acid-base catalysis conflicted with the possibility of redox catalysis as predicted by the presence of a dinucleotide binding site. we show that reduced fad i ...201020178989
[pantoea agglomerans bacteriemia in a liver transplant patient]. 201020187690
Displaying items 401 - 500 of 650