Publications

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sarcoptes scabiei infestation does not alter the stability of ectoparasite communities.the host represents a heterogeneous ecosystem where multiple parasite species co-occur and interact with each other for space and resources. although these interactions may rule the features of an infracommunity and may shape the infracommunity response to external perturbations, the resilience of ectoparasite communities to new infestations remains poorly explored.201627370780
anaplasma marginale and theileria annulata in questing ticks from portugal.ticks are ubiquitous arthropods and vectors of several pathogenic agents in animals and humans. monitoring questing ticks is of great importance to ascertain the occurrence of pathogens and the potential vector species, offering an insight into the risk of disease transmission in a given area. in this study 428 host-seeking ticks, belonging to nine species of ixodidae and collected from 17 of the 23 portuguese mainland subregions, were screened for several tick-borne agents with veterinary relev ...201627394441
seasonal dynamics of ixodid ticks on wild rabbits oryctolagus cuniculus (leporidae) from central spain.rabbit hemorrhagic disease and myxomatosis caused a decline in the rabbit population in the second half of the last century. despite some recovery, the risk of vector-borne disease is present and thus the importance of controlling vector populations. in the current study, we describe the ixodid tick fauna in wild rabbit in a natural reserve in ciudad real (central spain) during the course of two 3-year periods (2007-2009 and 2012-2014). of all the ticks collected on average 72.5 % were larvae, 2 ...201627411939
tick species, tick-borne pathogens and symbionts in an insular environment off the coast of western france.insular environments provide ideal natural conditions to study disease ecology, especially emerging diseases, due to clear differentiation between local and long-distance transmission. such environments are of particular interest regarding tick-borne pathogens (tbp), since animal exchange with the mainland (along with any ticks they carry) is limited, and because such locations could lie on migratory routes for birds carrying ticks. therefore both tick species and tbp may display different preva ...201627622976
species diversity and spatial distribution of ixodid ticks on small ruminants in greece.a cross sectional field study on the distribution of ixodidae ticks was carried out over two consecutive tick seasons (2012 and 2013) in small ruminants (309 farms) located in mainland and five islands of greece. a total of 2108 ticks (1199 females, 908 males, and 1 nymph) were collected from sheep and goats. two species of rhipicephalus, two of ixodes, five of hyalomma, three of haemaphysalis, and one of dermacentor were detected. the species with the widest distribution were rhipicephalus sang ...201627655133
bacterial pathogens and endosymbionts in ticks.ticks collected from goats in northern greece were tested for the presence of tick-borne bacteria. among adult ticks, 37 (57.8%) were rhipicephalus bursa, 11 (17.2%) dermacentor marginatus, 10 (15.6%) ixodes ricinus, 3 (4.7%) rhipicephalus sanguineus sensu lato and 2 (3.1%) haemaphysalis parva; one (1.6%) rhipicephalus spp. tick was nymph. rickettsia monacensis, rickettsia massilae, anaplasma phagocytophilum and anaplasma platys were detected in i. ricinus and rh. bursa ticks. a variety of coxie ...201727686386
assessment of domestic goats as models for experimental and natural infection with the north american isolate of rickettsia slovaca.rickettsia slovaca is a tick-borne human pathogen that is associated with scalp eschars and neck lymphadenopathy known as tick-borne lymphadenopathy (tibola) or dermacentor-borne necrosis erythema and lymphadenopathy (debonel). originally, r. slovaca was described in eastern europe, but since recognition of its pathogenicity, human cases have been reported throughout europe. european vertebrate reservoirs of r. slovaca remain unknown, but feral swine and domestic goats have been found infected o ...201627741322
introduction and persistence of tularemia in bulgaria.outbreaks of the zoonotic disease tularemia occurred in north-east bulgaria in the 1960s. then came 30 years of epidemiological silence until new outbreaks occurred in west bulgaria in the 1990s. to investigate how bacterial strains of francisella tularensis causing tularemia in wildlife and humans in the 1960s and the 1990s were related, we explored their genetic diversity.201627790972
ticks (acari: ixodidae) infesting cattle and some other domestic and wild hosts on the french mediterranean island of corsica.corsica is a mountainous french island in the north-western mediterranean presenting a large diversity of natural environments where many interactions between domestic animals and wild fauna occur. despite a favourable context for ticks and tick-borne diseases (tbds), the tick fauna of corsica has not systematically been investigated.201627842608
first report of rickettsia raoultii and r. slovaca in melophagus ovinus, the sheep ked.melophagus ovinus (diptera: hippoboscidae), a hematophagous ectoparasite, is mainly found in europe, northwestern africa, and asia. this wingless fly infests sheep, rabbits, and red foxes, and causes inflammation, wool loss and skin damage. furthermore, this parasite has been shown to transmit diseases, and plays a role as a vector. herein, we investigated the presence of various rickettsia species in m. ovinus.201627884170
pcr screening of tick-borne agents in sensitive conservation areas, southeast portugal.the southeast region of portugal, particularly the guadiana valley, is currently the reintroduction territory of lynx pardinus (iberian lynx), one of the most endangered felids in the world that is only found in the iberian peninsula. over the last century, populations have declined, placing l. pardinus at extremely high risk of extinction in the wild and relying on reintroduction projects. among the aspects taken into account in the establishment of new populations is the sanitary status of the ...201727894847
tick-borne diseases in turkey: a review based on one health perspective.the importance of tick-borne diseases is increasing all over the world, including turkey. global warming, environmental and ecological changes and the existence of suitable habitats increase the impact of ticks and result in frequent emergence or re-emergence of tick-borne diseases (tbds) with zoonotic characteristics. in turkey, almost 19 tbds have been reported in animals and men, involving four protozoa (babesiosis, theileriosis, cytauxzoonosis, hepatozoonosis), one filarial nematode (acantho ...201627977689
detection of rickettsia hoogstraalii, rickettsia helvetica, rickettsia massiliae, rickettsia slovaca and rickettsia aeschlimannii in ticks from sardinia, italy.tick-borne diseases represent a large proportion of infectious diseases that have become a world health concern. the presence of rickettsia spp. was evaluated by standard pcr and sequencing in 123 ticks collected from several mammals and vegetation in sardinia, italy. this study provides the first evidence of the presence of rickettsia hoogstralii in haemaphysalis punctata and haemaphysalis sulcata ticks from mouflon and rickettsia helvetica in ixodes festai ticks from hedgehog. in addition, ric ...201728110915
pyrosequencing based assessment of bacterial diversity in turkish rhipicephalus annulatus and dermacentor marginatus ticks (acari: ixodidae).ticks continue to be a threat to human and animal health in turkey, as they are considered important vectors of human and animal diseases. the objectives of this investigation are to characterize the microbial communities of two tick species, rhipicephalus annulatus and dermacenter marginatus, analyze patterns of co-occurrence among microbial taxa, identify and compare pathogens contributing human diseases, and determine whether avirulent symbionts could exclude human pathogens from tick communi ...201728111714
a comparative test of ixodid tick identification by a network of european researchers.this study reports the results of a comparative test of identification of ticks occurring in western europe and northern africa. a total of 14 laboratories were voluntarily enrolled in the test. each participant received between 22 and 25 specimens of adult and nymphal ticks of 11 species: dermacentor marginatus, d. reticulatus, haemaphysalis punctata, hyalomma lusitanicum, hy. marginatum, ixodes ricinus, i. hexagonus, rhipicephalus annulatus, r. bursa, r. rossicus, and/or r. sanguineus s.l. tic ...201728320640
molecular evidence of rickettsia spp. in ixodid ticks and rodents in suburban, natural and rural habitats in slovakia.natural foci of tick-borne spotted fever group (sfg) rickettsiae of public health concern have been found in slovakia, but the role of rodents in their circulation is unclear. ticks (ixodes ricinus, ixodes trianguliceps, dermacentor marginatus, dermacentor reticulatus, haemaphysalis concinna and haemaphysalis inermis) and tissues of rodents (apodemus flavicollis, apodemus sylvaticus, myodes glareolus, microtus arvalis, microtus subterraneus and micromys minutus) were examined for the presence of ...201728340608
molecular characterization of babesia and theileria species in ticks collected in the outskirt of monte romano, lazio region, central italy.in 2012-2013, an investigation was carried out in the viterbo province, lazio region, on ticks and tick-borne apicomplexan protozoa of the babesia and theileria genera. this followed the reporting of high density of ticks by soldiers operating in a military shooting range, and the signaling by owners and local veterinary authorities of several cases of babesiosis among cattle.201728361802
mesocarnivores and macroparasites: altitude and land use predict the ticks occurring on red foxes (vulpes vulpes).the red fox vulpes vulpes is the most common mesocarnivore in europe and with a wide geographical distribution and a high density in most terrestrial habitats of the continent. it is fast urbanising species, which can harbor high numbers of different tick species, depending on the region. here we present the results of a large-scale study, trying to disentangle the intricate relationship between environmental factors and the species composition of ectoparasites in red foxes. the samples were col ...201728381228
crimean-congo hemorrhagic fever virus in ticks in turkey: a broad range tick surveillance study.in turkey, crimean-congo hemorrhagic fever (cchf) outbreaks started in northern regions in 2002. human cases still continue to increase and the disease spreads in many other provinces of turkey. the ecological role of the vector tick species occurring in turkey is not certain exactly. therefore, we planned a broad range tick study in three different ecological and geographical areas extending from the west black sea regions down to the central anatolia. the aim of this study was to determine and ...201728433738
a retrospective study of the characterization of rickettsia species in ticks collected from humans.rickettsiae (family rickettsiaceae, order rickettsiales) are obligate intracellular bacteria transmitted by arthropod vectors. several rickettsia species causing vector-borne rickettsioses belong to the spotted fever group (sfg). traditionally, rickettsia conorii has been considered as the main etiologic agent of mediterranean spotted fever. however, the molecular characterization of rickettsiae allowed identifying other species involved in spotted fever in the mediterranean region. in this stud ...201728457821
rickettsia spp. in dermacentor marginatus ticks: analysis of the host-vector-pathogen interactions in a northern mediterranean area.in this work, we have analyzed the key features of rickettsia spp. maintenance in dermacentor marginatus ticks in four regions of the northern mediterranean area, where extensive investigations are currently lacking. for this purpose, 380 adult d. marginatus ticks were collected from vegetation and wild ungulates during a 3-year study. a host-vector-pathogen interaction approach was applied to investigate the key components of the disease systems and evaluate their respective weight in the inten ...201728500468
partial characterization of tick-borne encephalitis virus isolates from ticks of southern ukraine.tick-borne encephalitis (tbe) is the most common tick-borne viral infection in eurasia; thousands of human cases are annually reported from several european countries. several tick species are vectors of the tick-borne encephalitis virus (tbev), while tbe appears to be spreading from the eurasian continent westward to europe. fifteen study sites were chosen from five territories of southern ukraine, including odessa, mykolaiv, kherson oblast, the autonomous republic of crimea, and sevastopol. ti ...201728654319
diversity and distribution of ticks from domestic ruminants in lebanon.ticks (acari: ixodidae) are ectoparasites infesting livestock in every geographic area in the world and they are vectors of several viral, bacterial, and protozoan pathogens to animals and humans worldwide. a deep knowledge of the geographical distribution of these arthropods would have a key role in the control of tick-borne diseases. few data are available about tick presence in domestic ruminants in lebanon. the study aimed at providing an analysis of tick presence and distribution in lebanon ...201728675252
combination of rt-pcr and proteomics for the identification of crimean-congo hemorrhagic fever virus in ticks.crimean-congo hemorrhagic fever (cchf) is an emerging tick-borne zoonotic disease caused by the cchf virus (cchfv). in this study, an experimental approach combining rt-pcr and proteomics was used for the identification and characterization of cchfv in 106 ticks from 7 species that were collected from small ruminants in greece. the methodological approach included an initial screening for cchfv by rt-pcr followed by proteomics analysis of positive and control negative tick samples. this novel ap ...201728736753
one particular anaplasma phagocytophilum ecotype infects cattle in the camargue, france.anaplasma phagocytophilum is a zoonotic tick-borne pathogen responsible for granulocytic anaplasmosis, a mild to a severe febrile disease that affects man and several animal species, including cows and horses. in europe, i. ricinus is the only proven vector for this pathogen, but studies suggest that other tick genera and species could be involved in its transmission. our objective was to assess the presence and genetic diversity of a. phagocytophilum in domestic animals and different tick speci ...201728764743
molecular detection, infection rate and vectors of theileria lestoquardi in goats from west azerbaijan province, iran.this study was aimed to determine the infection rate and vectors of theileria lestoquardi in goats from west azerbaijan province, iran. a total of 400 blood samples were collected from 40 randomly selected flocks in the study area from june to september, 2014. out of 400 blood samples examined using microscopic examination, a number of 14 goats (3.50%) were positive for theileria spp., whereas 25 goats (6.25%) yielded a specific t. lestoquardi ssu-rrna fragment (235 bp). the prevalence of theile ...201728785390
tick-borne bacteria and protozoa detected in ticks collected from domestic animals and wildlife in central and southern portugal.ticks are vectors of many human and animal pathogens. the aim of this study was to screen bacteria and protozoa from ticks infesting domestic animals and wildlife collected in central and southern portugal. a total of 593 ticks, comprising 465 (78.4%) adults, 122 (20.6%) nymphs, and six (1.0%) larvae, were collected from 283 hosts of 25 different species (4 domestic and 21 wild). overall, the analysis of dna extracts prepared from ticks collected from hosts of 11 different species in the distric ...201728958791
spotted fever group rickettsiae associated with ixodid ticks in wild environment in southern italy.ixodidae ticks are vectors and reservoirs of several species of rickettsiae, and tick-borne rickettsioses are reported worldwide. this study was aimed to verify the distribution of spotted fever group rickettsiae associated with ticks in a wild environment, the national park of gargano, where there is proximity between wild and domestic animals, and which is within an endemic area for rickettsiosis. ticks were collected from animals or vegetation, morphologically identified and tested by a pcr t ...201729047217
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