Publications

TitleAbstractYear
Filter
PMID(sorted ascending)
Filter
cdr2p contributes to fluconazole resistance in candida dubliniensis clinical isolates.the development of resistance to azole antifungals used in the treatment of fungal infections can be a serious medical problem. here, we investigate the molecular mechanisms associated with reduced susceptibility to fluconazole in clinical isolates of candida dubliniensis , showing evidence of the trailing growth phenomenon. the changes in membrane sterol composition were studied in the presence of subinhibitory fluconazole concentrations. despite lanosterol and eburicol accumulating as the most ...201121542785
comparative in vitro susceptibility of clinical isolates of candida paparsilosis complex and other candida species to caspofungin and anidulafungin by etest.in vitro susceptibility of 141 clinical isolates of candida species to caspofungin and anidulafungin is reported. the etest was performed according to recommended procedure and minimum inhibitory concentrations (mics) were read after 24 h of incubation at 35°c. applying a breakpoint of <2 mg/ml, all candida spp. isolates, except those belonging to c. parapsilosis complex, were susceptible. the geometric mean for caspofungin and anidulafungin for different candida spp. were as follows: candida pa ...201121571626
in vitro antifungal evaluation and structure-activity relationship of diphenyl diselenide and synthetic analogues.we report on in vitro antifungal activity and the structure-activity relationship of diphenyl diselenide [(phse)(2) ] and its synthetic analogues, (p-cl-c(6) h(4) se)(2) , (m-cf(3) -c(6) h(4) se)(2) and (p-ch(3) o-c(6) h(4) se)(2) , against 116 strains of pathogenic fungi. (phse)(2) showed the highest inhibitory activity against candida albicans (minimum inhibitory concentration of 4-32 μg ml(-1) ), candida dubliniensis (2-16 μg ml(-1) ), aspergillus spp. (0.5-64 μg ml(-1) ) and fusarium spp. (2 ...201121615531
susceptibility of candida albicans and candida dubliniensis to erythrosine- and led-mediated photodynamic therapy.the effect of erythrosine- and led-mediated photodynamic therapy (pdt) on planktonic cultures and biofilms of candida albicans and candida dubliniensis was evaluated. planktonic cultures of standardized suspensions (10(6)cells/ml) of c. albicans and c. dubliniensis were treated with erythrosine concentrations of 0.39-200++m and leds in a 96-well microtiter plate. biofilms formed by c. albicans and c. dubliniensis in the bottom of a 96-well microtiter plate were treated with 400++m erythrosine an ...201121704304
activation of mapk/c-fos induced responses in oral epithelial cells is specific to candida albicans and candida dubliniensis hyphae.oral epithelial cells detect the human pathogenic fungus candida albicans via nf-+¦b and a bi-phasic mitogen-activated protein kinase (mapk) signaling response. however, discrimination between c. albicans yeast and hyphal forms is mediated only by the mapk pathway, which constitutes activation of the mapk phosphatase mkp1 and the c-fos transcription factor and is targeted against the hyphal form. given that c. albicans is not the only candida species capable of filamentation or causing mucosal i ...201121706283
prevalence of candida dubliniensis fungemia in argentina: identification by a novel multiplex pcr and comparison of different phenotypic methods.candida dubliniensis is an emerging pathogen that can cause invasive disease in patients who have a variety of clinical conditions. c. dubliniensis is often misidentified as candida albicans by clinical laboratories. in argentina, incidence data are still scarce, and only one systemic infection has been reported. this study aims to determine the prevalence of c. dubliniensis in blood samples in argentina, to evaluate a novel pcr multiplex as well as several phenotypic methods for the identificat ...201121750939
research on candida dubliniensis in a brazilian yeast collection obtained from cardiac transplant, tuberculosis, and hiv-positive patients, and evaluation of phenotypic tests using agar screening methods.the aim of this study was to research candida dubliniensis among isolates present in a brazilian yeast collection and to evaluate the main phenotypic methods for discrimination between c. albicans and c. dubliniensis from oral cavity. a total of 200 isolates, presumptively identified as c. albicans or c. dubliniensis obtained from heart transplant patients under immunosuppressive therapy, tuberculosis patients under antibiotic therapy, hiv-positive patients under antiretroviral therapy, and heal ...201121763095
first isolation of candida dubliniensis from oral cavities of dermatological patients in nanjing, china.candida dubliniensis is an emerging pathogen capable of causing both superficial and systemic infections. although c. dubliniensis and c. albicans are phenotypically similar, the two species differ in terms of epidemiology and the ability to rapidly develop resistance to fluconazole. c. dubliniensis is primarily associated with oral candidiasis of human immunodeficiency virus (hiv)-infected individuals. in this study, we describe the first recovery of c. dubliniensis from oral cavities of non-hi ...201121769632
differentiation between candida albicans and candida dubliniensis using hypertonic sabouraud broth and tobacco agar.opportunistic fungal infections in immunocompromised hosts are caused by candida species, and the majority of such infections are due to candida albicans. however, the emerging pathogen candida dubliniensis demonstrates several phenotypic characteristics in common with c. albicans, such as production of germ tubes and chlamydospores, calling attention to the development of stable resistance to fluconazole in vitro. the aim of this study was to evaluate the performance of biochemistry identificat ...201121860890
genotypic heterogeneity and molecular basis of 5-flucytosine resistance among candida dubliniensis isolates recovered from clinical specimens in kuwait.there is a paucity of information about genotypic heterogeneity among candida dubliniensis isolates recovered from different geographic regions. this study explored genotypic heterogeneity among 103 c. dubliniensis strains obtained over a six-year period from clinical specimens in kuwait. genotype assignment was based on amplification with genotype-specific primers and sequencing of rdna. susceptibility to 5-flucytosine was determined by means of the etest. dna sequencing of cytosine deaminase w ...201121895416
variation in biofilm formation among blood and oral isolates of candida albicans and candida dubliniensis.biofilm production is considered a potential virulence factor of some candida species. for this reason, an understanding of biofilm behavior of candida albicans and its closely related species candida dubliniensis is key to the development of effective preventive measures for invasive and oral candidiasis. the aim of this study was to compare the capacity of biofilm production by blood and oral isolates of c. albicans and c. dubliniensis using polystyrene, flat-bottomed 100-well microtiter plate ...201121899928
Triazole and echinocandin MIC distributions with epidemiological cutoff values for differentiation of wild-type strains from non-wild-type strains of six uncommon species of Candida.When clinical susceptibility breakpoints (CBPs) are absent, establishing wild-type (WT) MIC distributions and epidemiological cutoff values (ECVs) provides a sensitive means for detecting emerging resistance. We determined species-specific ECVs for anidulafungin (ANF), caspofungin (CSF), micafungin (MCF), fluconazole (FLC), posaconazole (PSC), and voriconazole (VRC) for six rarer Candida species (819 strains) using isolates obtained from the ARTEMIS Program and the SENTRY Antimicrobial Surveilla ...201121900519
Candida albicans versus Candida dubliniensis: Why Is C. albicans More Pathogenic?Candida albicans and Candida dubliniensis are highly related pathogenic yeast species. However, C. albicans is far more prevalent in human infection and has been shown to be more pathogenic in a wide range of infection models. Comparison of the genomes of the two species has revealed that they are very similar although there are some significant differences, largely due to the expansion of virulence-related gene families (e.g., ALS and SAP) in C. albicans, and increased levels of pseudogenisatio ...201221904553
distribution of yeast species associated with oral lesions in hiv-infected patients in southwest uganda.oropharyngeal candidiasis remains a significant clinical problem in hiv-infected and aids patients in regions of africa where anti-retroviral therapy isn't readily available. in this study we identified the yeast populations associated with oral lesions in hiv-infected patients in southwest uganda who were receiving treatment with nystatin and topical clotrimazole. samples were taken from 605 patients and 316 (52%) of these yielded yeast growth following incubation on sabouraud dextrose agar. sa ...201121905950
Candida dubliniensis endophthalmitis in a HIV-infected intravenous drug abuser. 201121929732
influence of doxorubicin on fluconazole susceptibility and efflux pump gene expression of candida dubliniensis.the effect of doxorubicin (dox) on the fluconazole (flu) susceptibility of c. dubliniensis was investigated. isolates were exposed to dox and flu in a chequerboard assay and resistance gene expressions were analysed after dox exposure. the susceptibility of the yeast to flu was decreased in the presence of dox in the chequerboard assay with fic indices suggesting an antagonistic effect. gene expression analyses showed an overexpression of cdcdr2. hence, dox was found to have an impact on resista ...201121939345
design, synthesis and antifungal activity of some new imidazole and triazole derivatives.triazole and imidazole are incorporated into the structures of many antifungal compounds. in this study a novel series of 1,2,4-triazole, imidazole, benzoimidazole, and benzotriazole derivatives was designed as inhibitors of cytochrome p450 14α-demethylase (14dm). these structures were docked into the active site of mt-cyp51, using autodock program. sixteen compounds with the best binding energy were synthesized. the chemical structures of the new compounds were confirmed by elemental and spectr ...201121984016
Do Hospital Microbiology Laboratories Still Need To Distinguish Candida albicans from Candida dubliniensis? 201121998427
identification of local clinical candida isolates using chromagar candida™ as a primary identification method for various candida species.the objective of our study was to study the effectiveness of chromagar candida™ as the primary identification method for various clinical candida isolates, other than the three suggested species by the manufacturer. we studied 34 clinical isolates which were isolated from patients in a local teaching hospital and 7 atcc strains. these strains were first cultured in sabouraud dextrose broth (sdb) for 36 hours at 35ºc, then on chromagar plates at 30ºc, 35ºc and 37ºc. the sensitivity of t ...201122041745
Evaluation of fluorescence in situ hybridisation (FISH) for the identification of Candida albicans in comparison with three phenotypic methods.Severe Candida infections are increasing and are associated with considerable morbidity and mortality. Rapid and accurate differentiation of Candida albicans from non-C. albicans species is essential for therapeutic decisions. We therefore developed a fluorescence in situ hybridisation (FISH) assay comprising previously described probes and a newly designed specific C. albicans probe/competitor probe combination. The FISH probes were first evaluated using 99 selected fungal strains covering 31 s ...201122126597
a simple xylose-based agar medium for the differentiation of candida dubliniensis and candida albicans.the utility of xylose-based agar medium for differentiation of candida dubliniensis from candida albicans is evaluated. all c. dubliniensis isolates failed to grow on this medium, while c. albicans isolates yielded good growth. this simple in-house medium offers an inexpensive alternative to commercial yeast identification systems for resource poor settings.201122209683
candida dubliniensis endophthalmitis: first case in north america.to report an unusual case of endogenous fungal endophthalmitis due to candida dubliniensis. interventional case report of a 27-year-old immunocompetent male with loss of vision, dense vitritis, and chorioretinal infiltrates, who underwent a diagnostic pars plana vitrectomy. microbiology cultures obtained by a diagnostic vitrectomy were positive for the growth of c. dubliniensis. this infectious process was then appropriately treated with intravitreal amphotericin b and systemic fluconazole with ...201222222717
determination of antifungal susceptibility patterns among the clinical isolates of candida species.candida species are opportunistic yeasts that cause infections ranging from simple dermatosis to potentially life-threatening fungemia. the emergence of resistance to antifungal drugs has been increased in the past two decades.201122223999
prevalence of oral candida carriage in thai adolescents.oral candidiasis is among the most common aids-associated opportunistic infections. adolescents remain at the highest risk of hiv infection and could suffer from oral candidiasis. however, information on oral candida carriage in this population is limited. this study aims to evaluate the prevalence of oral candida in thai adolescents.201122298521
antimicrobial properties and cytotoxicity of an antimicrobial monomer for application in prosthodontics.this study aimed to investigate the antimicrobial properties and cytotoxicity of the monomer methacryloyloxyundecylpyridinium bromide (mupb), an antiseptic agent capable of copolymerizing with denture base acrylic resins.201222339776
in vitro activities of antifungal agents alone and in combination against fluconazole-susceptible and -resistant strains of candida dubliniensis.in the present study we used two groups of candida dubliniensis strains: one containing fluconazole-susceptible clinical isolates and another containing fluconazole-resistant laboratory derivative from the former to examine the changes on susceptibility accompanying the development of resistance to fluconazole. our findings confirmed the ability of c. dubliniensis isolates to become resistant to fluconazole and indicated that this resistance was crossed with ketoconazole, itraconazole, ravuconaz ...201722358361
candida dubliniensis: an appraisal of its clinical significance as a bloodstream pathogen.a nine-year prospective study (2002-2010) on the prevalence of candida dubliniensis among candida bloodstream isolates is presented. the germ tube positive isolates were provisionally identified as c. dubliniensis by presence of fringed and rough colonies on sunflower seed agar. subsequently, their identity was confirmed by vitek2 yeast identification system and/or by amplification and sequencing of the its region of rdna. in all, 368 isolates were identified as c. dubliniensis; 67.1% came from ...201222396802
rapid differentiation of candida dubliniensis from candida albicans by early d-xylose assimilation.to determine if d-xylose (xyl) and/or α-methyl-d-glucoside (mdg) assimilation can be used reliably as a rapid test to differentiate candida dubliniensis from candida albicans at an earlier time point such as 2 h after inoculation.201222398877
potent antifungal activity of pure compounds from traditional chinese medicine extracts against six oral candida species and the synergy with fluconazole against azole-resistant candida albicans.this study was designed to evaluate the in vitro antifungal activities of four traditional chinese medicine (tcm) extracts. the inhibitory effects of pseudolaric acid b, gentiopicrin, rhein, and alion were assessed using standard disk diffusion and broth microdilution assays. they were tested against six oral candida species, candida albicans, candida glabrata, candida tropicalis, candida krusei, candida dubliniensis, and candida guilliermondii, including clinical isolates from hiv-negative, hiv ...201222454653
cell surface hydrophobicity of oral candida dubliniensis isolates following limited exposure to sub-therapeutic concentrations of chlorhexidine gluconate.candidal adhesion has been implicated as the initial step in the pathogenesis of oral candidiasis and cell surface hydrophobicity (csh) has been implicated in adhesion to mucosal surfaces. candida dubliniensis is an opportunistic pathogen associated with recurrent oral candidiasis. chlorhexidine gluconate is by far the commonest antiseptic mouth wash prescribed in dentistry. at dosage intervals the intraoral concentration of this antiseptic fluctuates considerably and reaches sub-therapeutic lev ...201322533484
the tlo proteins are stoichiometric components of candida albicans mediator anchored via the med3 subunit.the amplification of the tlo (for telomere-associated) genes in candida albicans, compared to its less pathogenic, close relative candida dubliniensis, suggests a role in virulence. little, however, is known about the function of the tlo proteins. we have purified the mediator coactivator complex from c. albicans (camediator) and found that tlo proteins are a stoichiometric component of camediator. many members of the tlo family are expressed, and each is a unique member of camediator. protein e ...201222562472
sequence and analysis of the genome of the pathogenic yeast candida orthopsilosis.candida orthopsilosis is closely related to the fungal pathogen candida parapsilosis. however, whereas c. parapsilosis is a major cause of disease in immunosuppressed individuals and in premature neonates, c. orthopsilosis is more rarely associated with infection. we sequenced the c. orthopsilosis genome to facilitate the identification of genes associated with virulence. here, we report the de novo assembly and annotation of the genome of a type 2 isolate of c. orthopsilosis. the sequence was o ...201222563396
the first korean case of candidemia due to candida dubliniensis.candidemia due to uncommon candida spp. appears to be increasing in incidence. c. dubliniensis has been increasingly recovered from individuals not infected with hiv. identification of c. dubliniensis can be problematic in routine clinical practice due to its phenotypic resemblance to c. albicans. we report the first case of c. dubliniensis candidemia in korea, which occurred in a 64-yr-old woman who presented with partial seizure, drowsiness, and recurrent fever. germ-tube positive yeast that w ...201222563560
molecular characterization of fungal populations on the tongue dorsum of institutionalized elderly adults.to characterize the global composition of oral fungal populations in frail elderly adults and to investigate the relationship with their health status.201222642872
the first documented case of candida dubliniensis leptomeningeal disease in an immunocompetent host.in june 2008, a 25-year-old man presented to the royal adelaide hospital, australia, with candida dubliniensis leptomeningeal disease. on examination the patient had focal neurological deficits, both sensory and motor. he tested negative for hiv and had normal t cell subsets. the patient was treated with 6 weeks of intravenous liposomal amphotericin b and oral flucytosine, and had a full recovery.201122687683
oral candidosis in lichen planus: the diagnostic approach is of major therapeutic importance.candida albicans is the most common fungal pathogen in humans, but other candida species cause candidosis. candida species display significant differences in their susceptibility to antimycotic drugs. patients with symptomatic or erythematous oral lichen planus (olp) commonly have candida infection requiring correct identification of candida species in order to initiate adequate antimycotic therapy. therefore, conventional cytosmear and culture tests were compared with genetic diagnostics on ora ...201322699660
[virulence of candida dubliniensis in comparison with candida albicans using an experimental model of mouse oral candiddiasis].certain species of candida are known as opportunistic fungal pathogens and candida albicans has especially been isolated oral candidiasis patients at high frequency as a result of its strong pathogenicity. recently c. dubliniensis is isolated mainly from immunocompromised patients, but is also detected from healthy persons. c. dubliniensis has similar cell morphology and molecular biological properties to c. albicans. thus, in order to clarify the pathogenicity of c. dubliniensis, the activities ...201222728597
comparative analysis of calcineurin signaling between candida dubliniensis and candida albicans.candida dubliniensis, an emerging fungal pathogen, is the closest known species to the established pathogenic species candida albicans. despite the fact that these two species share > 80% genome sequence identity, they exhibit distinct properties such as less hyphal growth, reduced pathogenicity and increased sensitivity to sodium stress and elevated temperatures in c. dubliniensis compared with c. albicans. it is, however, largely unknown whether signaling pathways are conserved in the two cand ...201222808313
stearidonic acid acts in synergism with amphotericin b in inhibiting candida albicans and candida dubliniensis biofilms in vitro. 201222817915
differential interaction of the two related fungal species candida albicans and candida dubliniensis with human neutrophils.candida albicans, the most common facultative human pathogenic fungus is of major medical importance, whereas the closely related species candida dubliniensis is less virulent and rarely causes life-threatening, systemic infections. little is known, however, about the reasons for this difference in pathogenicity, and especially on the interactions of c. dubliniensis with the human immune system. because innate immunity and, in particular, neutrophil granulocytes play a major role in host antifun ...201222851712
transcript profiling reveals rewiring of iron assimilation gene expression in candida albicans and c. dubliniensis.hyphal growth is repressed in candida albicans and candida dubliniensis by the transcription factor nrg1. transcript profiling of a c. dubliniensis nrg1 mutant identified a common group of 28 nrg1-repressed genes in both species, including the hypha-specific genes hwp1, ece1 and the regulator of cell elongation ume6. unexpectedly, c. dubliniensis nrg1 was required for wild-type levels of expression of 10 genes required for iron uptake including seven ferric reductases, sit1, ftr1 and rbt5. howev ...201222888912
predisposing factors for oropharyngeal colonization of yeasts in human immunodeficiency virus-infected patients: a prospective cross-sectional study.oropharyngeal candidiasis continues to be a major opportunistic infection in human immunodeficiency virus (hiv)-infected patients. the objectives of this study were to investigate the prevalence, associated factors, and microbiologic features for oropharyngeal yeast colonization in hiv-infected patients.201322921200
oral candida colonization in hiv-positive women: associated factors and changes following antiretroviral therapy.there is a paucity of information about the factors associated with oral colonization with candida species and the changes associated with antiretroviral (arv) therapy. this study investigated the role of arv therapy and other factors in a study population. relevant clinical and laboratory information was obtained and oral rinse specimens were tested for yeast identification. the findings were compared with previous data from the same clinic before arv therapy was available. of 197 patients, 117 ...201323002070
performance comparison of phenotypic and molecular methods for detection and differentiation of candida albicans and candida dubliniensis.candida albicans is the most pathogenic candida species but shares many phenotypic features with candida dubliniensis and may, therefore, be misidentified in clinical microbiology laboratories. candidemia cases due to c. dubliniensis are increasingly being reported in recent years. accurate identification is warranted since mortality rates are highest for c. albicans infections, however, c. dubliniensis has the propensity to develop resistance against azoles more easily. we developed a duplex pc ...201223009343
use of denaturing gradient gel electrophoresis for the identification of mixed oral yeasts in human saliva.a pcr-denaturing gradient gel electrophoresis (dgge) method was established for the simultaneous presumptive identification of multiple yeast species commonly present in the oral cavity. published primer sets targeting different regions of the saccharomyces cerevisiae 26-28s rrna gene (denoted primer sets n and u) and the 18s rrna gene (primer set e) were evaluated with ten candida and four non-candida yeast species, and twenty candida albicans isolates. optimized pcr-dgge conditions using prime ...201323065546
biological activities of pseudevernia furfuracea (l.) zopf extracts and isolation of the active compounds.pseudevernia furfuracea (l.) zopf (parmeliaceae) is a common epiphytic lichen in the conifer-hardwood forest of anatolia. this species is used in traditional medicine in turkey as a treatment for wounds, eczema and hemorrhoids.201223107822
the first case of candida dubliniensis endophthalmitis in north america. 201323132215
rapid and accurate identification of isolates of candida species by melting peak and melting curve analysis of the internally transcribed spacer region 2 fragment (its2-mca).rapid identification of clinically important yeasts can facilitate the initiation of anti-fungal therapy, since susceptibility is largely species-dependent. we evaluated melting peak and melting curve analysis of the internally transcribed spacer region 2 fragment (its2-mca) as an identification tool for distinguishing between 16 candida spp., i.e. candida albicans, candida bracarensis, candida dubliniensis, candida famata, candida glabrata, candida guilliermondii, candida inconspicua, candida k ...201323142490
effect of different pre-irradiation times on curcumin-mediated photodynamic therapy against planktonic cultures and biofilms of candida spp.the aim of this study was to evaluate the effects of pre-irradiation time (pit) on curcumin (cur)-mediated photodynamic therapy (pdt) against planktonic and biofilm cultures of reference strains of candida albicans, candida glabrata and candida dubliniensis.201323153629
differentiation of candida dubliniensis from candida albicans by means of maldi-tof mass spectrometry.species specific differentiation of the two closely related yeasts, candida albicans and c. dubliniensis, is difficult in routine diagnosis.201223163108
[yeast colonization of urinary catheters and the significance of biofilm formation].urinary catheters are colonized by a wide range of microorganisms, including numerous yeasts. the catheters are usually colonized by more microbial species forming a community - multispecies biofilm. catheter colonization usually does not affect the patient's clinical status in any significant way. on the other hand, the biofilm can become a source of endogenous infection and its presence can affect functionality of the catheter and formation of urinary stones. material a201223172023
prevalence of chronic hyperplasic candidiasis. its association to risk factors in an oral medicine service in rosario, argentina.chronic hyperplasic candidiasis (chc) is one of clinical forms of infection caused by fungi from genus candida, basically candida albicans, and less frequently by other species of the genus, such as candida tropicalis, candida guilliermondii, candida glabrata, candida parapsilosis, candida dubliniensis y candida krusei.201223177812
catheter-related fungemia caused by candida dubliniensis.infections caused by candida dubliniensis in humans are rare and have never been reported in taiwan. we report two cancer patients with catheter-related fungemia due to c. dubliniensis infection in taiwan. the two isolates were confirmed to the species level using an oligonucleotide array system and sequence analysis, and both showed high in vitro susceptibilities to nine antifungal agents. the catheters were removed, and both patients responded well to antifungal treatment. although this type o ...201323246304
oral candida species in head and neck cancer patients treated by radiotherapy.this paper aimed to identify and quantify candida on head and neck irradiated patients with two comparative elderly populations.201323260345
hydroxyurea therapy in sickle cell anemia patients aids to maintain oral fungal colonization balance.the aim of this study was to evaluate the frequency of candida species and presence of lesions in the oral cavity of patients with sickle cell anemia (ss).201223278631
invasive fungal infection and impaired neutrophil killing in human card9 deficiency.caspase recruitment domain-containing protein 9 (card9) is an adaptor molecule in the cytosol of myeloid cells, required for induction of t-helper cells producing interleukin-17 (th17 cells) and important in antifungal immunity. in a patient suffering from candida dubliniensis meningoencephalitis, mutations in the card9 gene were found to result in the loss of protein expression. apart from the reduced numbers of cd4(+) th17 lymphocytes, we identified a lack of monocyte-derived cytokines in resp ...201323335372
changes in germ tube formation and cell-surface hydrophobicity of oral candida dubliniensis isolates following brief exposure to sub-cidal concentrations of polyene and azole antifungal agents.adherence of candida has been implicated as the initial process in the pathogenesis of oral candidosis. candidal germ tubes and its relative cell-surface hydrophobicity (csh) are contributory attributes. candida dubliniensis is currently documented as an opportunistic pathogen allied with recurrent oral candidosis. oral candidosis can be treated with polyene and azole antifungals such as amphotericin b, ketoconazole and fluconazole. however, the intraoral concentration of these drugs fluctuates ...201323405864
[candida dubliniensis: need for correct diagnosis].a study of 115 strains, which had been isolated from vaginal smears and identified at the beginning as candida albicans, was conducted to find out if there was candida dubliniensis in cuba. the growth of those isolates that due to their morphological characteristics were identifified as either c. albicans or c. dubliniensis was checked up at 42 degrees in sabouraud agar. c. albicans was the predominant species (88.69%). for the first time in cuba, 13 c. dubliniensis strains were presumptively id ...201323424796
in vitro anticandidal evaluation of novel highly functionalized bis cyclohexenone ethyl carboxylates.novel highly functionalized bis cyclohexenone ethyl carboxylates 7-12 were designed, synthesized and their structures were elucidated by their elemental analysis, ms, ft-ir, one-dimensional 1h, and 13c nmr spectroscopic data.201323426531
synthesis of 1h-1,2,3-triazoles and study of their antifungal and cytotoxicity activities.we report herein the results of antifungal activity of fifteen 1,2,3-triazoles against candida albicans, candida krusei, candida parapsilosis, candida kefyr, candida tropicalis, candida dubliniensis, tricophyton rubrum, microporum canis and aspergillus niger. all of the 1,2,3-triazoles were prepared from 1,3-dipolar cyclizations between aryl azides and alkynes catalyzed by cu(i), and several of the compounds exhibited antifungal activity with low cytotoxicity. the results demonstrated the potent ...201323432315
efficient neocentromere formation is suppressed by gene conversion to maintain centromere function at native physical chromosomal loci in candida albicans.cenpa/cse4 assembles centromeric chromatin on diverse dna. cenpa chromatin is epigenetically propagated on unique and different centromere dna sequences in a pathogenic yeast candida albicans. formation of neocentromeres on dna, nonhomologous to native centromeres, indicates a role of non-dna sequence determinants in cenpa deposition. neocentromeres have been shown to form at multiple loci in c. albicans when a native centromere was deleted. however, the process of site selection for cenpa depos ...201323439889
comparison of cell wall proteins in putative candida albicans & candida dubliniensis by using modified staining method & sds-page.candida species are among the most common causes of opportunistic fungal diseases. among candida species, candida albicans is responsible for most infections. having many strains, c. albicans is very polymorph. c. dubliniensis is very similar to albicans species both morphologically and physiologically. for an infection to occur, cell wall proteins play an important role as they enable yeast to adhere to host cells and begin pathogenesis. therefore, we decided to extract these proteins and exami ...201223482280
[identification of three candida africana strains in senegal].the frequency of candidiasis has increased dramatically in recent years. candida albicans is the most common species. however, other species which are pathogenic and resistant to usual antifungal agents beginning to emerge. these include candida dubliniensis and candida africana, which share morphological similarities with candida albicans. thus, it is of interest to correctly identify the fungal isolates.201223518168
oral infections caused by yeasts in patients with head and neck cancer undergoing radiotherapy. identification of the yeasts and evaluation of their antifungal susceptibility.yeasts occur as part of the normal human microbiota. nevertheless, some species are opportunistic, affecting immunocompromised patients such as those undergoing oncologic treatment.201223518170
species distribution and virulence factors of candida spp. isolated from the oral cavity of kidney transplant recipients in brazil.although yeasts belonging to the genus candida are frequently seen as commensals in the oral cavity, they possess virulence attributes that contribute for pathogenicity. the aims of the present study were to study the prevalence of candida spp. isolated from the oral cavity of renal transplant recipients and to analyze strains virulence factors. we isolated a total of 70 candida strains from 111 transplant recipients, and candida albicans was the most prevalent species (82.86 %). oral candidiasi ...201323539354
photodynamic inactivation of yeast and bacteria by extracts of alternanthera brasiliana.this study was undertaken to evaluate the effect of alternathera brasiliana (amaranthaceae) extracts as photosensitizing agents in photodynamic antimicrobial therapies (pact) against staphylococcus aureus, staphylococcus epidermidis and candida dubliniensis. the crude hexane and ethanol extracts were obtained from a. brasiliana whole plant and showed absortion from 650 to 700 nm. also, singlet molecular oxygen (1o2) production (type ii photosensitization reaction) was examined, and the results s ...201323547779
species and condition specific adaptation of the transcriptional landscapes in candida albicans and candida dubliniensis.although candida albicans and candida dubliniensis are most closely related, both species behave significantly different with respect to morphogenesis and virulence. in order to gain further insight into the divergent routes for morphogenetic adaptation in both species, we investigated qualitative along with quantitative differences in the transcriptomes of both organisms by cdna deep sequencing.201323547856
global transcriptome sequencing identifies chlamydospore specific markers in candida albicans and candida dubliniensis.candida albicans and candida dubliniensis are pathogenic fungi that are highly related but differ in virulence and in some phenotypic traits. during in vitro growth on certain nutrient-poor media, c. albicans and c. dubliniensis are the only yeast species which are able to produce chlamydospores, large thick-walled cells of unknown function. interestingly, only c. dubliniensis forms pseudohyphae with abundant chlamydospores when grown on staib medium, while c. albicans grows exclusively as a bud ...201323613980
multicenter study evaluating the vitek ms system for identification of medically important yeasts.the optimal management of fungal infections is correlated with timely organism identification. matrix-assisted laser desorption ionization-time of flight (maldi-tof) mass spectrometry (ms) is revolutionizing the identification of yeasts isolated from clinical specimens. we present a multicenter study assessing the performance of the vitek ms system (biomérieux) in identifying medically important yeasts. a collection of 852 isolates was tested, including 20 candida species (626 isolates, includin ...201323658267
a stable hybrid containing haploid genomes of two obligate diploid candida species.candida albicans and candida dubliniensis are diploid, predominantly asexual human-pathogenic yeasts. in this study, we constructed tetraploid (4n) strains of c. albicans of the same or different lineages by spheroplast fusion. induction of chromosome loss in the tetraploid c. albicans generated diploid or near-diploid progeny strains but did not produce any haploid progeny. we also constructed stable heterotetraploid somatic hybrid strains (2n + 2n) of c. albicans and c. dubliniensis by spherop ...201323709179
the postantifungal effect of nystatin and its impact on adhesion attributes of oral candida dubliniensis isolates.the postantifungal effect (pafe) has an impact on candidal pathogenicity. however, there is no information on either the pafe or its impact on adhesion traits of oral candida dubliniensis isolates. oral candidosis can be treated topically with nystatin. adhesion to buccal epithelial cells (bec), germ tube (gt) formation and relative cell surface hydrophobicity (csh) are all colonisation attributes of candidal pathogenicity. hence, the main objective of this study was to investigate the in vitro ...201423773155
the prevalence of oral candida infections in periodontitis patients with type 2 diabetes mellitus.the purpose of this study was to determine the prevalence of candida spp. in periodontitis patients with type 2 diabetes mellitus.201323806705
comparison of the vitek 2 yeast susceptibility system with clsi microdilution for antifungal susceptibility testing of fluconazole and voriconazole against candida spp., using new clinical breakpoints and epidemiological cutoff values.a commercially available, fully automated yeast susceptibility test system (vitek 2; biomérieux, marcy d'etoile, france) was compared in 3 different laboratories with the clinical and laboratory standards institute (clsi) reference microdilution (bmd) method by testing 2 quality control strains, 10 reproducibility strains, and 425 isolates of candida spp. against fluconazole and voriconazole. reference clsi bmd mic endpoints and vitek 2 mic endpoints were read after 24 hours and 9.1-27.1 hours i ...201323870164
an in vitro study on the anti-adherence effect of brucea javanica and piper betle extracts towards oral candida.the adherence of candida to mucosal surfaces is the initial step for successful invasive process of the oral cavity. the study aimed to investigate the effect of two plant extracts on the non-specific and specific bindings of oral candida.201323915676
clinical isolates and laboratory reference candida species and strains have varying abilities to form biofilms.candida biofilms are a major virulence trait for this yeast. in this study, the biofilm-forming ability of the major medically important clinical and laboratory reference strains was compared. biofilms were quantified using traditional methods, that is, crystal violet (cv), tetrazolium (xtt) reduction and colony-forming unit assays (cfu), and two new methods: an automated cell counter (acc) and biofilm suspension turbidity (bst) method. biofilms could be categorized based on biofilm biomass (hig ...201323927631
endocarditis caused by candida dubliniensis.endocarditis caused by candida dubliniensis is a rare event and limited to few case reports. in this report, the authors present a patient with a history of intravenous drug use and hepatitis c and endocarditis involving a prosthetic aortic valve. also reviewed are the treatment guidelines for candida sp. endocarditis.201323974269
candida dubliniensis encrustation of an obstructing upper renal tract calculus.we present the case of a 53-year-old man, with a history of alcohol abuse, requiring intensive care unit admission, with an obstructing right upper renal calculus and klebsiella pneumoniae urosepsis. ureteroscopic treatment of this obstruction displayed a small calculus within the renal pelvis completely encapsulated within a fungal bezoar. laboratory analysis of the fungal mass found it to be candida dubliniensis.201323975908
a simple and reliable pcr-restriction fragment length polymorphism assay to identify candida albicans and its closely related candida dubliniensis.candida dubliniensis is an emerging pathogen capable of causing superficial as well as systemic infections. due to its close similarity to c. albcians, conventional methods based on phenotypic traits are not always reliable in identification of c. dubliniensis. in this study, we developed a pcr-restriction fragment length polymorphism (rflp) assay to identify and discriminate between the two closely related species. the d1/d2 region of 28s rdna was amplified by pcr and enzymatically digested by ...201224031901
anabolic steroids affect human periodontal health and microbiota.this study aims to evaluate periodontal microbiological differences between systemically healthy nonsmoker males taking anabolic androgenic steroids (aass) and non-aas users and to find associations between disease severity and aas use.201424221579
third case of candida dubliniensis endogenous endophthalmitis in north america: case report and review of the literature.there are two previous reports of candida dubliniensis endophthalmitis in north america. here, we report a third case of c. dubliniensis endogenous endophthalmitis in a 31-year-old male patient who complained of left-sided decreased visual acuity. he had an associated mitral and tricuspid valve endocarditis, in the setting of intravenous drug use. blood and sputum cultures were positive for c. dubliniensis. fundoscopic examination was consistent with a fungal endophthalmitis. he was treated with ...201424234425
candida dubliniensis endophthalmitis: five cases over 15 years.recent studies have shown that the recently identified organism candida dubliniensis is less pathogenic than the more common candida albicans. due to its rare nature, c. dubliniensis has been previously reported as the causative organism in endophthalmitis in only three cases. we undertook a multicenter, retrospective, consecutive case series to describe the clinical features and outcomes of patients with culture-proven c. dubliniensis endophthalmitis. medical records were reviewed for all patie ...201324252588
molecular epidemiology, phylogeny and evolution of candida albicans.a small number of candida species form part of the normal microbial flora of mucosal surfaces in humans and may give rise to opportunistic infections when host defences are impaired. candida albicans is by far the most prevalent commensal and pathogenic candida species. several different molecular typing approaches including multilocus sequence typing, multilocus microsatellite typing and dna fingerprinting using c. albicans-specific repetitive sequence-containing dna probes have yielded a wealt ...201424269341
antifungal susceptibility and growth inhibitory response of oral candida species to brucea javanica linn. extract.candida species have been associated with the emergence of resistant strains towards selected antifungal agents. plant products have been used traditionally as alternative medicine to ease candidal infections. the present study was undertaken to investigate the antifungal susceptibility patterns and growth inhibiting effect of brucea javanica seeds extract against candida species.201324305010
purpurin triggers caspase-independent apoptosis in candida dubliniensis biofilms.candida dubliniensis is an important human fungal pathogen that causes oral infections in patients with aids and diabetes mellitus. however, c. dubliniensis has been frequently reported in bloodstream infections in clinical settings. like its phylogenetically related virulent species c. albicans, c. dubliniensis is able to grow and switch between yeast form and filamentous form (hyphae) and develops biofilms on both abiotic and biotic surfaces. biofilms are recalcitrant to antifungal therapies a ...201324376900
in vitro activity of natural phenolic compounds against fluconazole-resistant candida species: a quantitative structure-activity relationship analysis.to evaluate the antifungal activity and to analyse the structure-activity relationship of eleven natural phenolic compounds against four candida species which are resistant to fluconazole.201424387763
the usefulness of dna sequencing after extraction by whatman fta filter matrix technology and phenotypic tests for differentiation of candida albicans and candida dubliniensis.since c. dubliniensis is similar to c. albicans phenotypically, it can be misidentified as c. albicans. we aimed to investigate the prevalence of c. dubliniensis among isolates previously identified as c. albicans in our stocks and to compare the phenotypic methods and dna sequencing of d1/d2 region on the ribosomal large subunit (rlsu) gene. a total of 850 isolates included in this study. phenotypic identification was performed based on germ tube formation, chlamydospore production, colony colo ...201424436014
calcineurin controls hyphal growth, virulence, and drug tolerance of candida tropicalis.candida tropicalis, a species closely related to candida albicans, is an emerging fungal pathogen associated with high mortality rates of 40 to 70%. like c. albicans and candida dubliniensis, c. tropicalis is able to form germ tubes, pseudohyphae, and hyphae, but the genes involved in hyphal growth machinery and virulence remain unclear in c. tropicalis. recently, echinocandin- and azole-resistant c. tropicalis isolates have frequently been isolated from various patients around the world, making ...201424442892
paediatric oropharyngeal and cutaneous candidiasis with special reference to candida dubliniensis.mucocutaneous and cutaneous candidiasis, though common in children, is often under-reported. the prevalence of candida dubliniensis in causing these infections in this age group is also largely unknown. a prospective epidemiological cross-sectional study for candidiasis was performed in paediatric patients clinically suspected of candidiasis with oropharyngeal lesions (75 patients), cutaneous lesions (18 patients) and lesions at both sites (2 patients). candida species were identified by convent ...201424445508
fluconazole and amphotericin-b resistance are associated with increased catalase and superoxide dismutase activity in candida albicans and candida dubliniensis.candida dubliniensis, a new species of candida that has been recovered from several sites in healthy people, has been associated with recurrent episodes of oral candidiasis in aids and hiv-positive patients. this species is closely related to c. albicans. the enzymatic activity of c. dubliniensis in response to oxidative stress is of interest for the development of drugs to combat c. dubliniensis.201424474018
the effect of psychoactive substances (drugs) on the presence and frequency of oral candida species and candida dubliniensis.the goal of this study was to determine the effect of psychoactive substances (drugs) on the presence and frequency of oral candida species and candida dubliniensis.201324511261
candida dubliniensis spondylodiscitis in an immunocompetent patient. case report and review of the literature.we describe what appears to be the first case of spondylodiscitis due to candida dubliniensis. our case adds to the current literature of the importance of c. dubliniensis as a cause of fungemia and subsequent deep seated infections. it highlights the importance of taking fungal as well as bacterial culture from biopsy specimens in patients with spondylodiscitis. we also review the literature covering the reported cases of candida spondylodiscitis, which amount to about 100 over the last 5 decad ...201424567891
oral candida carriage and immune status in thai human immunodeficiency virus-infected individuals.oral candidiasis is a common opportunistic infection among human immunodeficiency virus (hiv)-infected individuals, with growing concerns about the emergence of non-albicans species with resistance to antifungal agents. this cross-sectional study determined the prevalence of oral candida species in thai hiv-infected adults and factors affecting their colonization. candida species were identified from oral rinse samples of 60 hiv-infected participants of the mtct-plus initiative and 49 healthy co ...201424591706
comparative adherence of candida albicans and candida dubliniensis to human buccal epithelial cells and extracellular matrix proteins.candida albicans and candida dubliniensis are very closely related pathogenic yeast species. despite their close relationship, c. albicans is a far more successful colonizer and pathogen of humans. the purpose of this study was to determine if the disparity in the virulence of the two species is attributed to differences in their ability to adhere to human buccal epithelial cells (becs) and/or extracellular matrix proteins. when grown overnight at 30°c in yeast extract peptone dextrose, genotype ...201424625677
the development and validation of a rapid genetic method for species identification and genotyping of medically important fungal pathogens using high-resolution melting curve analysis.accurate, rapid and economical fungal species identification has been a major aim in mycology. in this study, our goal was to examine the feasibility of a high-resolution melting curve analysis (hrma) of internal transcribed regions its1 and its2 in ribosomal dna (rdna) for a rapid, simple and inexpensive differentiation of eight clinically relevant candida species (candida albicans, candida glabrata, candida parapsilosis, candida krusei, candida tropicalis, candida guilliermondii, candida dubli ...201424628973
association of clinical and demographic factors in invasive candidiasis caused by fluconazole-resistant candida species: a study in 15 hospitals, medellín, colombia 2010-2011.candida is the most important agent of fungal infections. several risk factors have been described associated with invasive infection by fluconazole-resistant candida spp. a prospective cross-sectional study with case-control analysis was conducted. case group patients with fluconazole-resistant candida isolate were included; control group were patients with fluconazole-susceptible candida spp. a multivariate logistic regression model was performed. three hundred isolates of candida spp. were an ...201424666706
species distribution and antifungal susceptibility profile of candida isolates from bloodstream infections in lima, peru.yeast identification and in vitro susceptibility testing provide helpful information for appropriate administration of antifungal treatments; however, few reports from the latin american region have been published. the aim of this study was to identify the species present in isolates from bloodstream infections diagnosed in nine hospitals in lima, peru and to determine their in vitro susceptibility to four antifungal drugs. we tested and identified 153 isolates collected between october 2009 and ...201424667770
functional annotation of putative hypothetical proteins from candida dubliniensis.an extensive analysis of c. dubliniensis proteomics data showed that ~22% protein are conserved hypothetical proteins (hps) whose function is still not determined precisely. analysis of gene sequence of hps provides a platform to establish sequence-function relationships to a more profound understanding of the molecular machinery of organisms at systems level. here we have combined the latest versions of bioinformatics tools including, protein family, motifs, intrinsic features from the amino ac ...201424704023
multi-drug resistant oral candida species isolated from hiv-positive patients in south africa and cameroon.candida species are a common cause of infection in immune-compromised hiv-positive individuals, who are usually treated with the antifungal drug, fluconazole, in public hospitals in africa. however, information about the prevalence of drug resistance to fluconazole and other antifungal agents on candida species is very limited. this study examined 128 candida isolates from south africa and 126 cameroonian candida isolates for determination of species prevalence and antifungal drug susceptibility ...201424726686
high prevalence of candida dubliniensis in lower respiratory tract secretions from cystic fibrosis patients may be related to increased adherence properties.we identified candida spp isolated from lower respiratory tract secretions obtained from cystic fibrosis (cf) patients, by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms), with the aim of determining the most prevalent causative agent. we also sought to determine their adhesive properties in order to understand their biology related to cf.201424780917
effect of eugenol on cell surface hydrophobicity, adhesion, and biofilm of candida tropicalis and candida dubliniensis isolated from oral cavity of hiv-infected patients.most candida spp. infections are associated with biofilm formation on host surfaces. cells within these communities display a phenotype resistant to antimicrobials and host defenses, so biofilm-associated infections are difficult to treat, representing a source of reinfections. the present study evaluated the effect of eugenol on the adherence properties and biofilm formation capacity of candida dubliniensis and candida tropicalis isolated from the oral cavity of hiv-infected patients. all isola ...201424799938
Refresh
firstprev
123456
nextlast
Displaying items 401 - 500 of 599