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typing of hospital strains of xanthomonas maltophilia by pulsed-field gel electrophoresis.we report the use of pulsed-field gel electrophoresis (pfge) to characterize xanthomonas maltophilia isolates from an incident of hospital-acquired infection over a 12-day period in a haematological unit. ten isolates from five patients (from throat, urine, stool and blood) and two isolates from environmental sites in the unit were compared with 10 epidemiologically unrelated clinical strains, isolated over a 3-month period from several units in two hospitals, by pfge of drai digests of chromoso ...19947963462
[purpura fulminans complicating pneumococcal sepsis: a case report].an unusual case of a 67-year-old man is reported with fulminant pneumococcal sepsis. he had been healthy before, and the identified predisposing factors were only that he was a chronic alcohol drinker and was a hcv carrier. he presented signs of acute renal failure, liver dysfunction, adult respiratory distress syndrome and disseminated intravascular coagulation. subsequently purpura fulminans (symmetrical peripheral gangrene) with major extremity involvement developed. he finally survived with ...19947963803
[nosocomial respiratory infection caused by xanthomonas maltophilia in immunocompromised hosts].between january 1988 and december 1992, 68 patients admitted to our department of internal medicine with hematological malignancies or solid tumors showed colonization of the respiratory tract with xanthomonas maltophilia (x. maltophilia). to characterize the significance of respiratory tract colonization by x. maltophilia, we retrospectively reviewed the medical records of the 68 patients colonized with this organism. twenty-seven of these 68 patients developed pneumonia, with x. maltophilia be ...19947967236
mucocutaneous and soft tissue infections caused by xanthomonas maltophilia. a new spectrum.to describe the mucocutaneous and soft tissue infections caused by xanthomonas maltophilia in patients with cancer.19947978724
meningitis due to xanthomonas maltophilia: case report and review.xanthomonas maltophilia is being increasingly recognized as an opportunistic pathogen in debilitated patients. we report a case of postoperative meningitis due to x. maltophilia and review the cases of x. maltophilia meningitis reported in the literature. because x. maltophilia is often resistant to multiple beta-lactam agents, including cephalosporins and imipenem, trimethoprim-sulfamethoxazole appears to be the drug of choice for treatment of x. maltophilia meningitis.19947986908
the antibiotic susceptibilities of xanthomonas maltophilia and their relation to clinical management.an increase in recovery of xanthomonas maltophilia from clinical specimens at our institutions prompted, amongst other measures, an investigation of the antibiotic susceptibility patterns of the organism. fifty-five consecutive first isolates of xanthomonas maltophilia were obtained and antimicrobial susceptibility tests were carried out by the agar dilution method. trimethoprim/sulfamethoxazole was the most active antimicrobial agent (94% susceptible), with 71% susceptible to ticarcillin/clavul ...19947991292
bacterial colonisation with xanthomonas maltophilia--a retrospective study in a cystic fibrosis patient population.xanthomonas maltophilia was isolated from 25 of 150 patients with cystic fibrosis during a period of 10 years (1983-1992). twelve patients harboured x. maltophilia chronically, i.e. repeatedly for more than 6 months. no predisposing factors for the colonisation could be identified by studying the clinical and laboratory data of the patients, including preceding and concurrent bacterial colonisation with other bacteria, antibacterial treatments, pulmonary function and biochemical markers. up to 2 ...19948002085
the bacteriology of obstructive pneumonitis. a prospective study using ultrasound-guided transthoracic needle aspiration.obstructive pneumonitis, the opacity that develops distal to an obstructing endobronchial lesion or external compression, is actually a combination of atelectasis, bronchiectasis with mucus plugging, and true parenchymal inflammation. in the majority of cases, it is usually not possible to determine whether infection is present or not from the radiographic findings alone. the aim of this study was to evaluate the bacteriology of obstructive pneumonitis and the influence of this result on the tre ...19948004324
[a new threat: xanthomonas maltophilia infection. apropos of 50 cases].in an attempt to further characterize infections due to xanthomonas maltophilia (xm), we reviewed 50 case reports observed in our institution. xm is emerging as an important nosocomial pathogen in immunocompromised patients, especially those receiving broad spectrum antimicrobial antibiotherapy. distinction between colonisation and infection is often difficult. xm presents a therapeutic challenge because of its tendency to exhibit multiple resistance.19938009079
[fulminant purpura in still's disease and bacteremia with xanthomonas maltophilia and coagulase-negative staphylococci].we describe the case of a 31-year-old man with a long history of juvenile rheumatoid arthritis who was admitted to the hospital because of painful purple skin lesions on hands and feet. on admission he presented the classical picture of "purpura fulminans" with extensive acrocyanosis and large blisters on the lower limbs which evolved into symmetrical peripheral gangrene. laboratory findings revealed activated intravascular coagulation and bacteremia with coagulase-negative staphylococci and xan ...19948016605
sequence analysis of the l1 metallo-beta-lactamase from xanthomonas maltophilia.the amino acid sequence deduced from the l1 beta-lactamase gene of xanthomonas maltophilia shows a significant variation from that of the cpha and blm metallo-beta-lactamases of aeromonas hydrophila and bacillus cereus, respectively. whilst the n-terminus of the l1 protein shows some similarity, particularly at the histidine residues previously suggested as a zinc-binding motif, the c-terminus of the protein demonstrates very little similarity. such differences amongst this group of enzymes woul ...19948018721
antimicrobial activity of a new antipseudomonal dual-action drug, ro 25-0534.ro 25-0534, a tertiary amine-linked dual action combination (dac) of a catechol cephalosporin and ciprofloxacin, was compared with a previously described dac (ro 23-9424), ciprofloxacin and cefotaxime. a total of 688 recent clinical isolates were tested and an additional collection of 110 gram-negative bacilli possessing documented resistance to broad-spectrum antimicrobials were used. ro 25-0534 was active against all tested species of enterobacteriaceae (mic90 range, 0.06-2 micrograms/ml), oxa ...19948026159
changing etiology of bacteremia in patients with hematological malignancies in denmark.to ascertain whether the microbiological etiology of bacteremia among patients with hematological malignancies has changed in denmark, the species distribution of clinically relevant blood culture isolates from the hematological department at rigshospitalet, copenhagen in 1990 was compared with 2 previous studies (1970-72; 1981-85). in addition, time trends of the etiology of bacteremia among hematological patients in copenhagen (eastern denmark) and in arhus (western denmark) were compared. in ...19948036471
xanthomonas maltophilia bacteremia in immunocompromised hematological patients.epidemiological, microbiological and clinical characteristics of 14 episodes of xanthomonas maltophilia bacteremia in 12 seriously immunocompromised hematological patients, admitted to rigshospitalet in copenhagen over the 3-year period 1989-91, were evaluated. the results were compared with a randomly selected control group of 25 patients with escherichia coli bacteremia. hospital acquired bacteremia was more common among the patients with x. maltophilia bacteremia (p < 0.01). treatment with br ...19948036472
outer membrane protein profiles of xanthomonas maltophilia isolates displaying temperature-dependent susceptibility to gentamicin. 19948040133
in vitro antimicrobial activity of cp-99,219, a novel azabicyclo-naphthyridone.cp-99,219 is a trifluoronaphthyridone with significant antibacterial activity that includes the family enterobacteriaceae (mics for 90% of the strains tested [mic90s], < or = 0.015 to 0.5 micrograms/ml), moraxella catarrhalis, haemophilus influenzae, and gonococci (mics, < or = 0.015 micrograms/ml). legionella spp. were also cp-99,219 susceptible, with mics of 0.008 to 0.12 micrograms/ml. cp-99,219 demonstrated activity greater than that of ciprofloxacin, ofloxacin, or enoxacin against pseudomon ...19938043036
meningitis due to xanthomonas maltophilia.during 1st week of post-operative period, a 28 year old female patient operated for left cerebellopontine angle tumor, continued to get fever. lumbar puncture did not reveal any organisms. she responded to ciprofloxacin. two months later, she was readmitted with signs and symptoms of meningitis. the csf tapped on lumbar puncture grew xanthomonas maltophilia, gram negative bacilli, sensitive to various antibiotics, ciprofloxacin being one of them. the patient was given ciprofloxacin for 3 weeks. ...19938051648
analysis of dicamba degradation by pseudomonas maltophilia using high-performance capillary electrophoresis.a method based on high-performance capillary electrophoresis (hpce) was developed for the simultaneous analysis of dicamba and its metabolites in media containing the bacterium pseudomonas maltophilia. dicamba, 3,6-dichlorosalicylic acid (dcsa), and related products were extracted from media samples using ether under acidic conditions and injected onto an hpce system containing a ph 10.0 running buffer. baseline resolution between these compounds was obtained at 30 kv with a total run time of 6 ...19948059953
rapid identification of metallo- and serine beta-lactamases.simple methods to detect, identify, and differentiate metallo- and serine beta-lactamases were developed and used to differentiate enzymes produced by 17 clinical isolates of xanthomonas maltophilia. all isolates exhibited beta-lactamase activity, and in 16 strains this was induced by imipenem. all but one isolate hydrolyzed imipenem (and meropenem), and in all cases this activity was inhibited by 1 mm edta. the metallo- and serine beta-lactamases in the cell extracts were distinguished on isoel ...19948067782
controlled clinical evaluation of isolator and esp aerobic blood culture systems for detection of bloodstream infections.a controlled clinical evaluation comparing the isolator system (wampole laboratories, cranbury, n.j.) and the esp 80a blood culture bottle in the automated esp system (difco laboratories, detroit, mich.) was performed with 10,535 blood culture sets from patients with suspected septicemia. of 1,150 positive cultures, 844 positive cultures from 285 patients with 394 septic episodes fulfilled the study criteria for minimum blood sample requirements in each system and clinical significance of isolat ...19948077401
occurrence and antibiotic-resistance of pseudomonas species isolated from drinking water in southern greece.a total of 194 samples of drinking waters consisting of 88 tap waters and 106 non-carbonated bottled waters were processed for isolation of pseudomonas species during a 4-month period according to standard methods. pseudomonas aeruginosa was the predominant isolated pseudomonas species. twenty-eight (14.4%) p. aeruginosa were isolated from 194 samples. eight (9%) were isolated from 88 tap water samples and 20 (18.8%) from 106 bottled water samples. eight (9%) tap waters yielded non-p. aeruginosa ...19948077985
levofloxacin in vitro activity: results from an international comparative study with ofloxacin and ciprofloxacin.levofloxacin, the s-(-)-isomer of ofloxacin, was compared to ofloxacin and ciprofloxacin against > 6000 recent clinical isolates of gram-positive and gram-negative bacteria from six different countries. this international multicenter study demonstrated a high level of antibacterial activity of levofloxacin against all the members of enterobacteriaceae [minimum inhibitory concentration (mic)50s, < or = 0.03 to 0.12 mg/l] except providencia rettgeri (mic50, 2 mg/l), and providencia stuartii (mic50 ...19948077990
in vitro comparison activity of opc-17116, a new fluoroquinolone, against more than 5,000 recent clinical isolates from five medical centers.the in vitro activity of opc-17116, a new fluoroquinolone, was compared with those of ciprofloxacin, ofloxacin and cefixime. a total of 5,231 fresh clinical isolates from five geographically separate us medical centers were tested. the primary results of this study are: 1. opc-17116 is very active against enterobacteriaceae [mic90s, 0.015-1 microgram/ml, except providencia rettgeri (mic90, > 8 micrograms/ml)], 2. among nonenteric gram-negative bacilli (e.g. xanthomonas maltophilia, mic90, 2 micr ...19938106899
differential binding capacity and internalisation of bacterial substrates as factors in growth rate of acanthamoeba spp.acanthamoeba spp. are free-living predators that selectively feed on bacteria. adherence of the bacterial food source to the trophozoite membrane is followed by internalisation and digestion. through co-cultivation of a. castellanii and a. polyphaga, individually, with xanthomonas maltophilia, escherichia coli, staphylococcus epidermidis and pseudomonas aeruginosa (despite the amoebicidal properties of the latter organism), specificity with regard to the preferred bacterial substrate was judged. ...19948107064
in vitro activity of du-6859a, a new fluorocyclopropyl quinolone.du-6859a was tested against 844 recent clinical isolates (most from bacteremias) by using reference mic determination procedures. the activity of du-6859a against members of the family enterobacteriaceae was comparable to that of ciprofloxacin (range of mics for 90% of isolates [mic90], < or = 0.015 to 1 microgram/ml), and the highest mics were observed among serratia marcescens and providencia rettgeri isolates. the du-6859a mic90 for pseudomonas aeruginosa and xanthomonas maltophilia was 0.5 m ...19938109948
in vitro activity of three carbapenem antibiotics. comparative studies with biapenem (l-627), imipenem, and meropenem against aerobic pathogens isolated worldwide.the in vitro activity of biapenem (formerly l-627 or ljc10, 627), a new carbapenem, was compared with that of imipenem and meropenem against > 6000 clinically significant pathogens isolated in six countries worldwide. biapenem was active against members of the family enterobacteriaceae with a 90% minimum inhibitory concentration (mic90) ranging from < or = 0.06 to 2 micrograms/ml. only serratia spp. and providencia spp. were less susceptible (mic90, 8 micrograms/ml). pseudomonas aeruginosa and x ...19938112045
zinc ions and medium-dependent susceptibility to beta-lactams in xanthomonas maltophilia. 19948157562
characterization and purification of the chorionic gonadotropin-like protein binding site in candida albicans.recent studies from our laboratory have shown that human chorionic gonadotropin (hcg), human luteinizing hormone (hlh), and cg-like proteins from xanthomonas maltophilia (xcg) and candida albicans (cacglp) induce candida albicans transition from blastospores to hyphal forms. xanthomonas maltophilia cg-like protein (xcg), hcg, and hlh also bind to candida albicans blastospores with a high-affinity nm kd, indicating that these substances can control candida albicans pathogenicity. the work reporte ...19948168460
[xanthomonas maltophilia endocarditis in an aids patient]. 19948182994
susceptibilities of 123 xanthomonas maltophilia strains to clinafloxacin, pd 131628, pd 138312, pd 140248, ciprofloxacin, and ofloxacin.the susceptibilities of 123 clinically isolated strains of xanthomonas maltophilia to six fluoroquinolones (clinafloxacin, pd 131628, pd 138312, pd 140248, ciprofloxacin, and ofloxacin) were examined by microdilution mic methodology. clinafloxacin and pd 131628 were the most active compounds tested (mics for 50% of the strains tested [mic50s] of 0.5 and 1.0 microgram/ml and mic90s of 2.0 and 4.0 micrograms/ml, respectively). pd 138312, pd 140248, ciprofloxacin, and ofloxacin were less active, wi ...19948192468
a changing pattern of susceptibility of xanthomonas maltophilia to antimicrobial agents: implications for therapy.the in vitro susceptibilities of 130 xanthomonas maltophilia isolates to 12 antibiotics--trimethoprim-sulfamethoxazole, minocycline, ticarcillin-clavulanate, ceftazidime, cefoperazone, cefoperazone-sulbactam, imipenem, ciprofloxacin, and the investigational quinolones pd 117558, pd 117596, pd 127391, and sparfloxacin--were determined by a microtiter broth dilution technique. other than the investigational quinolones, the most active antibiotics were minocycline, trimethoprim-sulfamethoxazole, an ...19948203865
multicentre survey of the comparative in-vitro activity of piperacillin/tazobactam against bacteria from hospitalized patients in the british isles.twenty-nine british and irish hospitals each collected up to 300 bacterial isolates from in-patients. the organisms were identified by an appropriate api system or, for staphylococci, by their gram and coagulase reactions. disc susceptibility tests were performed. isolates that gave zones < or = 25 mm to piperacillin/tazobactam (75 micrograms + 10 micrograms) discs were sent to a central laboratory for re-examination and determination of mic, together with a sample of the more susceptible organi ...19938226427
in vitro activity of ro 23-9424, a dual-acting cephalosporin-quinolone antimicrobial agent.in vitro activity of new dual-acting antibacterial ro 23-9429 was tested against 1294 bacterial isolates from patients in a major tertiary care referral hospital in saudi arabia. its activity was compared with that of ciprofloxacin, fleroxacin, ampicillin, cephalothin, cefoxitin, cefotaxime, ceftazidime, piperacillin, oxacillin, gentamicin, amikacin, imipenem, and vancomycin. of the 621 members of enterobacteriaceae tested, every single isolate was inhibited by ro 23-9429 at minimum inhibitory c ...19938227462
[nosocomial sepsis caused by xanthomonas maltophilia]. 19938231365
an outbreak of gram-negative bacteremia traced to contaminated o-rings in reprocessed dialyzers.to investigate an outbreak of gram-negative bacteremia in an outpatient hemodialysis unit and to identify the source of contaminating bacteria and the route by which bacteria gained access to the bloodstream.19938239225
empirical antibiotic therapy for fever in neutropenic patients.the necessity for administering empirical antibiotic therapy to febrile neutropenic patients has been well-established; however, no single regimen has been uniformly accepted. during the past decade, gram-positive organisms, often methicillin-resistant, have emerged as significant pathogens. the routine use of vancomycin may be appropriate at some institutions, but its widespread use may lead to resistance. the role of aminoglycosides as part of routine empirical regimens is controversial, and s ...19938274604
a comparative study of the in-vitro activity of cefepime and other cephalosporins.cefepime is a new cephalosporin antibiotic which is highly active against both gram-positive and gram-negative organisms. the purpose of this study was to establish the in-vitro activity of cefepime and three other cephalosporins against recent clinical isolates from patients at the general hospital kuala lumpur. a total of 334 strains comprising enterobacteriaceae, non-fermentative gram-negative bacilli and staphylococcus aureus were tested for their sensitivity to cefepime, cefotaxime, ceftria ...19938277793
[mucinase activity of helicobacter pylori: application of simplified mucinase test].we conducted the simplified mucinase tests about helicobacters (helicobacter pylori, helicobacter felis and helicobacter mustelae) and campylobacter jejuni, positive controls were pseudomonas maltophilia and pseudomonas cepacia. h. pylori, h. felis and c. jejuni showed low resolution activities for hog gastric mucin, however h. mustelae had no detectable the activities. this enzymatic activity varies considerably by cultural condition of h. pylori, therefore it is difficult to compare the activi ...19938283626
in vitro activity of bay y 3118, a new quinolone.mics of bay y 3118, ciprofloxacin, ofloxacin, clarithromycin, azithromycin, cefuroxime, amoxicillin-clavulanate, and trimethoprim-sulfamethoxazole for 878 recent clinical isolates were determined by broth microdilution methods. among the three quinolones, bay y 3118 was the most active against haemophilus influenzae, moraxella catarrhalis, acinetobacter baumannii, xanthomonas maltophilia, gram-positive cocci, and anaerobes; mics for 50% of the strains (mic50s) and mic90s were < or = 0.015 and < ...19938285618
pulmonary clearance and inflammatory response in c3h/hej mice after intranasal exposure to pseudomonas spp.the environmental release of engineered microorganisms has caused health and environmental concerns. in this study, an animal model was used to examine health effects following pulmonary exposure to environmental and clinical isolates. in order to rule out the possibility that an adverse response was caused by endotoxin, 50% lethal doses (ld50) were determined, when possible, with endotoxin-sensitive (c3heb/fej) and endotoxin-resistant (c3h/hej) mice by using both environmental isolates (pseudom ...19938285667
infection problems for the 1990's--do we have an answer?there is and has been continued change in organisms causing infection in the hospital. in the past few years, although gram-negative bacteria have remained a major cause of mortality, gram-positive bacteria and fungi have become increasingly important. this has caused organisms such as methicillin-resistant staphylococci, enterococci, xanthomonas maltophilia and multiply resistant pseudomonas aeruginosa to be common pathogens. can this difficult state of affairs be changed by better antimicrobia ...19938290904
[ciprofloxacin resistance in gram negative bacilli. epidemiologic aspects].the aim of the present was to study the resistance to ciprofloxacin (cip) in gram negative bacilli (april 1990-march 1992) and to determine the temporary distribution of the resistant strains by species, samples and departments.19938305553
a bacterial protein has homology with human chorionic gonadotropin (hcg).studies from our laboratory have demonstrated the presence of a 48.5 kd cell wall protein in the bacterium, xanthomonas maltophilia, which immunologically resembles the beta subunit of human chorionic gonadotropin. primers were designed from the amino acid sequences of enzymatically cleaved peptide fragments of this protein. these primers were used to obtain pcr amplified products, which were subsequently cloned in a pcr11ta cloning vector, and a 492 base pair nucleotide sequence was obtained wi ...19938323559
stenotrophomonas, a new bacterial genus for xanthomonas maltophilia (hugh 1980) swings et al. 1983.in consideration of the criticisms of the transfer of pseudomonas maltophilia to the genus xanthomonas proposed by j. swings, p. de vos, m. van den mooter, and j. de ley (int. j. syst. bacteriol. 33:409-413, 1983), a new generic name is created for this taxon. the name stenotrophomonas is here proposed for the new genus, which includes a single species, stenotrophomonas maltophilia. this proposal restores the genus xanthomonas to its former definition (j. bradbury, p. 199-210, in n. r. krieg and ...19938347518
community-acquired xanthomonas maltophilia pyelonephritis.we have described the case of a patient with a community-acquired urinary tract infection with what is more typically a nosocomial pathogen. our patient had not been hospitalized recently, and his only established risk factor for x maltophilia infection was chronic bladder catheterization. although paraplegic and thus disabled, he was not debilitated. he had had no recent antibiotic therapy prior to the onset of symptoms, but he did have a history of multiple previous bladder catheter-related in ...19938351564
antimicrobial activity of the new carbapenem biapenem compared to imipenem, meropenem and other broad-spectrum beta-lactam drugs.the in vitro activity of biapenem was compared to that of imipenem, meropenem and other broad-spectrum beta-lactams. a total of 716 isolates from recent cases of clinical septicemia and an additional 137 stock strains possessing known beta-lactamases or other well-characterized resistance mechanisms were tested. the minimal concentrations inhibiting 90% of strains (mic90) of enterobacteriaceae species were for biapenem 0.03 to 1 mg/l and for imipenem 0.25 to 2 mg/l. no member of the enterobacter ...19938354307
in vitro antimicrobial activity of cp-99433 compared with other fluoroquinolones.cp-99433 is a new c-7 diazabicyclofluoroquinolone with a broad spectrum of activity that includes enterobacteriaceae, gram-positive cocci, and nonenteric gram-negative bacilli. potent activity was demonstrated against pseudomonas aeruginosa [minimum inhibitory concentration (mic90, 0.5 microgram/ml), xanthomonas maltophilia (mic90, 1 microgram/ml), and all streptococcus species (mic90s, 0.25-1 microgram/ml). only cp-99433 was active against enterococcus faecium (mic50, 2 micrograms/ml). cp-99433 ...19938359012
in vitro antibacterial activity and beta-lactamase stability of cp-70,429 a new penem antibiotic.in in vitro susceptibility tests, the new penem cp-70,429 showed potent antibacterial activity against gram-positive and gram-negative bacteria except pseudomonas aeruginosa and xanthomonas maltophilia. cp-70,429 was stable to various types of beta-lactamases except for the enzyme from x. maltophilia and was 16- to 128-fold more active than the other compounds against beta-lactamase-producing strains of enterobacter cloacae and citrobacter freundii.19938363389
in-vitro susceptibilities of pseudomonas aeruginosa and pseudomonas spp. to the new fluoroquinolones clinafloxacin and pd 131628 and nine other antimicrobial agents.the in-vitro activities of two new fluoroquinolones, clinafloxacin (ci-960, pd 127391, am-1091) and pd 131628 (the active component of the pro-drug ci-990, pd 131112) and nine other antibiotics were tested against 107 clinical isolates of pseudomonas aeruginosa, 12 isolates of xanthomonas maltophilia, and 19 isolates of other pseudomonas spp. of the 107 p. aeruginosa isolates, 33 were resistant to gentamicin, tobramycin and amikacin, 17 were resistant to only one or two of these aminoglycosides ...19938390434
inhibition of adherence of mucoid pseudomonas aeruginosa by alginase, specific monoclonal antibodies, and antibiotics.the adherence of pseudomonal species was investigated by using a newly developed radiometric dacron fiber microcolumn assay. pseudomonas aeruginosa, p. stutzeri, and xanthomonas maltophilia were more adherent (approximately 20%) than p. pseudomallei, p. fluorescens, and p. cepacia (approximately 10%). mucoid strains of p. aeruginosa were consistently more adherent than nonmucoid strains (30% versus 20%). alginase was shown to inhibit the adherence of mucoid but not nonmucoid p. aeruginosa. monoc ...19938406822
characterization of a human chorionic gonadotropin-like protein from candida albicans.studies from our laboratory have demonstrated that human cg (hcg), human lh, (hlh), and an hcg-like protein extracted from xanthomonas maltophilia were able to induce candida albicans transition from the blastospore to the germ tube stage. in the present study, we describe the characterization of an hcg-like material extracted from candida albicans blastospores (cacglp), which is potent in inducing transition and presumably represents the endogenous transition-inducing substance. this material w ...19938425486
partial nucleotide sequence of the xanthomonas maltophilia chorionic gonadotropin-like receptor.xanthomonas maltophilia possesses a unique high-affinity binding site which binds human chorionic gonadotropin (hcg), but not human luteinizing hormone (hlh) or other glycoprotein hormones. we designed primers from the known nucleotide sequence of the human lh/cg receptor, spanning an area extending from transmembrane region 2 to transmembrane region 6. genomic dna extracted from xanthomonas maltophilia was used to obtain a pcr amplified product using the above primers. the primary amplification ...19938427582
telluria mixta (pseudomonas mixta bowman, sly, and hayward 1988) gen. nov., comb. nov., and telluria chitinolytica sp. nov., soil-dwelling organisms which actively degrade polysaccharides.pseudomonas mixta (type strain, acm 1762 [= atcc 49108], an actively dextranolytic species that possesses both lateral and polar flagella, was compared with the strictly aerobic, rod-shaped, chitinolytic bacterium "pseudomonas chitinolytica" acm 3522t (= cncm i-804) (t = type strain), which has a similar flagellation pattern, by performing phenotypic characterization and dna-dna hybridization studies and by analyzing dna base compositions and 16s rrna sequences. our results indicated that "p. ch ...19938427803
biphenomycin c, a precursor of biphenomycin a in mixed culture.a precursor of biphenomycin a in mixed culture of streptomyces griseorubiginosus no. 43708 with pseudomonas maltophilia no. 1928 was isolated and characterized. the structure of the precursor, designated biphenomycin c was determined to be a peptide which is composed of biphenomycin a and arginylserine residue (fig. 1), on the basis of chemical and spectroscopic evidence.19938436546
effect of divalent cations in bacteriological media on the susceptibility of xanthomonas maltophilia to imipenem, with special reference to zinc ions.the susceptibility of xanthomonas maltophilia strains to beta-lactams is known to vary according to the type of growth media used. we have assayed the divalent cation content of various susceptibility testing media and correlated this with the susceptibility to imipenem of 30 strains of x. maltophilia, by calculating the ic50. no correlation was found with ca++ and mg++ content (r = 0.005, p = 0.99), but a highly significant correlation with the zn++ content (7.7-42.7 mumol/l) of the medium was ...19938444674
ro 23-9424, a new cephalosporin 3'-quinolone: in-vitro antimicrobial activity and tentative disc diffusion interpretive criteria.the susceptibility of 410 clinical bacterial isolates to ro 23-9424, a novel cephalosporin 3'-quinolone, was determined. overall, 97% of enterobacteriaceae and 100% of staphylococci were inhibited by < or = 8.0 mg/l of ro 23-9424. only 60% of pseudomonas aeruginosa and 80-90% of pseudomonas spp. and xanthomonas maltophilia were inhibited by this concentration. enterococci and listeria monocytogenes were resistant to ro 23-9424. clinical isolates of moraxella catarrhalis, streptococcus spp., and ...19938444677
activity of the carbapenem panipenem and role of the oprd (d2) protein in its diffusion through the pseudomonas aeruginosa outer membrane.evidence of permeation of panipenem through the oprd (d2) channel of pseudomonas aeruginosa outer membrane was shown by using oprd protein-producing and -nonproducing strains which contained plasmid phn4, which codes for l-1 beta-lactamase of xanthomonas maltophilia. permeation by panipenem was determined by measuring hydrolysis of the carbapenem by beta-lactamase in the periplasmic space. permeation by panipenem was also determined by counting uptake of [14c]panipenem into p. aeruginosa pao1 an ...19938452364
in vitro activity of cefdinir (fk 482, pd 134393, ci-983): a new orally active cephalosporin.cefdinir is a new orally active cephalosporin which is undergoing in vitro and in vivo evaluations. using the standard agar dilution method we compared the in vitro activity of this drug with other beta-lactam antibiotics against clinical isolates or enterobacteriaceae (625 strains), pseudomonas aeruginosa (68 strains), xanthomonas maltophilia (36 strains), acinetobacter (52 strains), aeromonas hydrophilia (47 strains), staphylococci (364 strains) and enterococci (50 strains). against most membe ...19938458243
in vitro activity and beta-lactamase stability of fk-037, a parenteral cephalosporin.the in vitro activity of fk-037, 5-amino-2-[[(6r, 7r)-7-[[(z)-2-(2-amino-4-thiazolyl)-2- methoxyimino) acetyl] amino]-2-carboxy-8-oxo-5-thia-1- azabicyclo[4.2.0]oct-2-en-3-yl]methyl]-1-(2-hydroxyethyl)-1h-pyrazoli um hydroxide, inner salt, sulfate (1:1), a new parenteral cephem, was compared with those of cefepime, ceftazidime, imipenem, and ciprofloxacin. fk-037 inhibited methicillin-susceptible staphylocci at < or = 4 micrograms/ml. of 98 isolates of homogenous methicillin-resistant staphyloco ...19938460925
degradation of acrylamide by immobilized cells of a pseudomonas sp. and xanthomonas maltophilia.two bacterial isolates capable of utilizing acrylamide as the sole source of carbon and nitrogen were isolated from herbicide-contaminated soil samples and identified as pseudomonas sp. and xanthomonas maltophilia. batch cultures of pseudomonas sp. and x. maltophilia completely degraded 62.8 mm acrylamide to acrylic acid and ammonia in 24 and 48 h, respectively. pseudomonas sp. and x. maltophilia, when immobilized in calcium alginate, markedly increased the rate of degradation of acrylamide over ...19938467421
an overview of the kinetic parameters of class b beta-lactamases.the catalytic properties of three class b beta-lactamases (from pseudomonas maltophilia, aeromonas hydrophila and bacillus cereus) were studied and compared with those of the bacteroides fragilis enzyme. the a. hydrophila beta-lactamase exhibited a unique specificity profile and could be considered as a rather specific 'carbapenemase'. no relationships were found between sequence similarities and catalytic properties. the problem of the repartition of class b beta-lactamases into sub-classes is ...19938471035
a natural isolate of pseudomonas maltophila which degrades aromatic sulfonic acids.a natural isolate, designated bsa56, which was originally selected for growth with benzene sulfonic acid as sole carbon and energy source, was identified as a strain of pseudomonas maltophila. strain bsa56 grew on a wide range of aromatic sulfonic acids and was shown to release sulfite from benzene sulfonic acid and 2-naphthalene sulfonic acid. although it also grew on toluene sulfonic acid and pyridine sulfonic acid, no significant sulfite release was observed with these substrates. release of ...19938472898
xanthomonas maltophilia peritonitis in a patient undergoing peritoneal dialysis. 19938491665
synthesis and antibacterial activity of cephalosporins having a catechol in the c3 side chain.cephalosporins having a catechol through a variety of linkages to the c3 position and a different c7 side chain were prepared. among them, 3-(catechol-4- ylcarbonyloxy)methylcephalosporin (14) and 3-[(e)-3-(catechol-4-ylcarbonyloxy)-1-propen-1-yl]cephalospo rin (10) showed excellent activity against gram-negative activity including ceftazidime-resistant escherichia coli, pseudomonas aeruginosa, xanthomonas maltophilia and pseudomonas cepacia.19938514638
biochemical properties of a carbapenem-hydrolyzing beta-lactamase from enterobacter cloacae and cloning of the gene into escherichia coli.a clinical isolate of enterobacter cloacae, strain nor-1, exhibited resistance to imipenem and remained susceptible to extended-spectrum cephalosporins. clavulanic acid partially restored the susceptibility of the strain to imipenem. two beta-lactamases with isoelectric points (pi) of 6.9 and > 9.2 were detected in strain e. cloacae nor-1; the higher pi corresponded to ampc cephalosporinase. plasmid dna was not detected in e. cloacae nor-1 and imipenem resistance could not be transferred into es ...19938517720
growth temperature-dependent variation of cell envelope lipids and antibiotic susceptibility in stenotrophomonas (xanthomonas) maltophilia.clinical isolates of stenotrophomonas (xanthomonas) maltophilia showed growth temperature-dependent variation in susceptibility (tdvs) to aminoglycoside antibiotics between 30 degrees c and 37 degrees c, but little or no tdvs effect for polymixin b, colistin, ceftazidime, chloramphenicol and piperacillin. when phenylethanol was added at sub-inhibitory concentrations, the tdvs effect was eliminated. gas liquid chromatography showed that 13-methyl tetradecanoate (i-15;0), was the predominant fatty ...19958522461
a multicenter comparative study of the in vitro activity of fleroxacin and other antimicrobial agents.the in vitro activity of fleroxacin was determined by broth microdilution against 2,079 recent bacterial isolates and compared to the activities of ciprofloxacin, ofloxacin, lomefloxacin, cefaclor, cefuroxime, cefixime, ceftriaxone, amoxicillin/clavulanate, trimethoprim/sulfamethoxazole (tmp-smx), and, as appropriate, erythromycin and oxacillin. most enterobacteriaceae were inhibited by the quinolones at a concentration of < or = 1 microgram/ml; mic90s of fleroxacin, ciprofloxacin, ofloxacin, an ...19958529440
prolidase from xanthomonas maltophilia: purification and characterization of the enzyme.prolidase (iminodipeptidase, ec 3.4.13.9) was purified from an extract of xanthomonas maltophilia, by ammonium sulfate fractionation and sequential chromatographies on deae-toyopearl, toyopearl hw65c, fplc-hiload superdex 200 pg, and fplc-hitrap q columns, which an activity recovery of 2.3%. the enzyme was the most active at ph 7.5 with leu-pro as substrate. it was stable between ph 6.0 and 8.5 for 60 min at 37 degrees c and retained half of activity after 60 min at 37 degrees c. the isoelectric ...19958541647
[cystic fibrosis: a microbiological study over an 8-year period].to study the microbiology of cystic fibrosis in our hospital for the period from 1985 to 1992.19958542180
in vitro activity of ofloxacin, ciprofloxacin, and cefoperazone alone and in combination against xanthomonas maltophilia. 19958549319
comparison of susceptibility to extended-spectrum beta-lactam antibiotics and ciprofloxacin among gram-negative bacilli isolated from intensive care units.the in vitro activities of extended-spectrum beta-lactam antibiotics (including piperacillin, cefotaxime, ceftriaxone, ceftazidime, cefepime, imipenem, and meropenems) were assessed and compared with the activity of ciprofloxacin against 366 clinical gram-negative bacilli isolates from the intensive care units of taichung veterans general hospital. the most prevalent species isolated were pseudomonas aeruginosa and acinetobacter baumannii. the activities of ceftazidime, cefepime, imipenem, and m ...19958565418
stenotrophomonas maltophilia in cystic fibrosis patients. 19958565998
physical structure and expression of alkba encoding alkane hydroxylase and rubredoxin reductase from pseudomonas maltophilia.the structural genes of the pseudomonas maltophilia alk system, which are localized on the oct plasmid were cloned as a 4.2-kilobase pair hind iii fragment. this fragment contains sequences for alkane hydroxylase gene (alkb) and rubredoxin reductase gene (alka), respectively. the alkb gene encodes a 373-amino acid polypeptide (47.4 kd) that can be expressed at high levels in pseudomonas and escherichia coli. the alkba genes were complemented with alkane hydroxylation in both bacteria. this resul ...19968573125
analysis of restriction fragment length polymorphism and ribotyping of multiresistant stenotrophomonas maltophilia isolated from persisting lung infection in a cystic fibrosis patient.a cystic fibrosis patient was infected by stenotrophomonas maltophilia over at least 15 months. the bacteria became increasingly resistant to antimicrobial agents. determination of restriction fragment length polymorphism and ribotyping showed that resistance was due to changes in that s. maltophilia rather than to infection by another strain.19958588142
levels and identities of nonrhizobial microorganisms found in commercial legume inoculant made with nonsterile peat carrier.sixty samples of commercial north american legume inoculants manufactured for sale in 1994 using nonsterile peat as carrier were tested for rhizobium (or bradyrhizobium) content and non-rhizobium biological contaminant load. products of three major producers of such inoculants for sale in canada were examined. viable rhizobium content varied from 5.6 x 10(5) to 8.1 x 10(9) cells/g, while the contaminant load varied from 1.8 x 10(8) to 5.5 x 10(10) cfu/g. most of the inoculants contained more non ...19968595599
purification and characterization of a newly screened microbial peptide amidase.a microbial peptide amidase was found in a limited screening and purified about 500-fold from stenotrophomonas maltophilia. the native enzyme has a molecular mass of 38 kda (gel filtration). the sequence of the first 16 amino acids was determined by edman degradation. the isoelectric point was found to be around 5.8. the peptide amidase exhibited a ph optimum of 6.0 and a temperature optimum of about 39-45 degrees c. the enzyme is stable in 50 mm tris/hcl, ph 7.5, at 30 degrees c, and the residu ...19958597540
molecular nosocomial epidemiology: high speed typing of microbial pathogens by arbitrary primed polymerase chain reaction assays.arbitrary primed polymerase chain reaction (ap pcr) assays are suited for the discrimination of isolates of all clinically relevant bacterial species. in a hospital setting, this type of dna amplification test can be used for the timely detection of ongoing nosocomial outbreaks. for rapid screening of isolates of many medically important bacterial species, including acinetobacter baumannii, staphylococcus aureus, serratia marcescens, xanthomonas maltophilia and others, a single ap pcr assay can ...19958601687
stenotrophomonas maltophilia ocular infections.to determine if the number of ocular infections associated with stenotrophomonas maltophilia is increasing, to identify predisposing factors, and to evaluate antimicrobial susceptibility.19968602781
transfer of ceftazidime and aztreonam resistance from nosocomial strains of xanthomonas (stenotrophomonas) maltophilia to a recipient strain of pseudomonas aeruginosa ml-1008. 19958605913
stenotrophomonas (xanthomonas) maltophilia urinary tract infection. a disease that is usually severe and complicated.stenotrophomonas (xanthomonas) maltophilia has emerged as a causative agent of serious nosocomial infections. however, well-documented cases of urinary tract infection with this organism have rarely been reported.19968607729
in vitro activities of antimicrobial combinations against stenotrophomonas (xanthomonas) maltophilia.stenotrophomonas (xanthomonas) maltophilia is inherently resistant to multiple antimicrobial agents. in order to investigate the in vitro potential of combinations of antimicrobial agents, we obtained 230 epidemiologically unrelated clinical isolates from seven hospitals across canada and from northwestern memorial hospital in chicago. ticarcillin-clavulanate combined with ciprofloxacin or trimethoprim-sulfamethoxazole were assayed for synergy against 31 ticarcillin-resistant strains of s. malto ...19958619571
antimicrobial activity of cs-940, a new trifluorinated quinolone.the antimicrobial activity of cs-940, a new trifluorinated quinolone drug, was tested against 761 clinical isolates. cs-940 activity against members of the family enterobacteriaceae was most similar to that of ciprofloxacin and ofloxacin, with a large range of mics inhibiting 90% of isolates tested (mic90s) of 0.015 to 16 micrograms/ml (median mic90, 0.06 micrograms/ml). cs-940 had greater activity than ciprofloxacin or ofloxacin when they were tested against acinetobacter spp. (mic90s, 0.03 mic ...19958619590
in vitro susceptibility of 124 xanthomonas maltophilia (stenotrophomonas maltophilia) isolates: comparison of the agar dilution method with the e-test and two agar diffusion methods.the in vitro susceptibility of 124 xanthomonas maltophilia isolates was tested by four methods: agar dilution (reference method), e-test, a disk diffusion and a tablet diffusion method. trimethoprim-sulfamethoxazole had the highest activity against x. maltophilia, followed by a combination of aztreonam-clavulanic acid at different ratios, the ratio 1:1 being the most active with a susceptibility rate of 85% as compared to 2% for aztreonam alone. addition of the beta-lactamase inhibitor tazobacta ...19968619912
isepamicin (sch 21420, 1-n-hapa gentamicin b): microbiological characteristics including antimicrobial potency of spectrum of activity.isepamicin (formerly sch 21420 or 1-n-hapa-gentamicin b) is a novel broad-spectrum aminoglycoside which possesses a high level of stability to aminoglycoside inactivating enzymes and low levels of toxicity to the kidney and inner ear. the only modifying enzymes capable of inactivating isepamicin are ant(4')-i (staphylococci), ant(4')-ii and aph(3')-vi, in addition to resistance mediated by permeability mutations. the spectrum of isepamicin is most similar to that of amikacin, another aminoglycos ...19958622113
stenotrophomonas maltophilia: an unusual cause of biliary sepsis.we report three cases of cholangitis caused by stenotrophomonas maltophilia and review two other cases reported in the literature. all five episodes occurred in patients with hepatobiliary malignancy complicated by biliary tract obstruction. all five episodes occurred in patients with hepatobiliary malignancy complicated by biliary tract obstruction. all patients had undergone biliary tract instrumentation. before infection developed, four of the five patients had received therapy with antibioti ...19958645796
relapsing pyrogenic reactions due to xanthomonas maltophilia in a dialysis patient with a long-term central venous catheter. 19968649636
xanthomonas maltophilia and pseudomonas cepacia in lower respiratory tracts of patients in critical care units.xanthomonas maltophilia and pseudomonas cepacia are gram-negative bacilli that are considered to opportunistic pathogens. these bacteria may cause colonization and infection, especially in acutely ill patients. between 1 july 1990 and 30 june 1992 sputum [correction of suptum] culture results from patients in the critical care units were surveyed daily. during the 2 year period, sputum from 27 patients grew x. maltophilia. it was hospital-acquired in 26 patients. a total of 26 patients were mech ...19958666858
[hospital pneumonia due to xanthomonas maltophilia and aids]. 19968696654
sensitivity of l-2 type beta-lactamases from stenotrophomonas maltophilia to serine active site beta-lactamase inhibitors. 19968707755
[pathogenic bacteria isolated from the sputum of the patients with pulmonary emphysema].isolated pathogenic bacteria from sputum of the patients with pulmonary emphysema who were admitted in our hospital from 1984 to 1994 were examined to elucidate the relationship between isolated bacteria from sputum and pulmonary functions including vital capacity (vc), forced expiratory volume (fev1.0), pao2 and paco2. vc of the patients from whom mssa (methicillin-sensitive staphylococcus aureus) or enterococcus faecalis (e. faecalis) were isolated was significantly lower than that of the pati ...19958708405
clinical and microbiological observations of early polytetrafluoroethylene membrane exposure in guided bone regeneration. case reports in primates.premature membrane exposure at one week is described in 3 macaca mulatta monkeys as part of a sequence of experiments on guided bone regeneration. clinical sequelae include redness, edema, and tissue slough. bacteroides fragilis, streptococcus pneumoniae, prevotella intermedia, and staphylococcus intermedius were detected at all prematurely exposed sites. pseudomonas maltophilia, strept, pneumoniae, and p. intermedia were the predominant organisms detected and consisted of more than 10% of the t ...19968708956
resistant nosocomial gram-negative bacillary pathogens: acinetobacter baumannii, xanthomonas maltophilia, and pseudomonas cepacia. 19968714248
a low-copy number plasmid mediating beta-lactamase production by xanthomonas maltophilia.to delineate the mechanisms contributing to the high level of antimicrobial resistance often demonstrated by xanthomonas maltophilia, plasmid dna was isolated from 5 clinical isolates and analyzed. purified plasmid dna from a single isolate contained a 6.5 kb plasmid (pxm222) and a 5.6 kb plasmid, (pthb). transformation of pthb into e. coli hb101 resulted in the expression of resistance to all penicillins tested and cefazolin.19958718603
structure of the o3 antigen of stenotrophomonas (xanthomonas or pseudomonas) maltophilia.the o antigen isolated from the lipopolysaccharide of a strain of stenotrophomonas (xanthomonas or pseudomonas) maltophilia serogroup o3 was found to contain 4-acetamido-4,6-dideoxy-d-galactose, d-fucose, and n-acetyl-d-glucosamine. by means of chemical degradations and nmr spectroscopy the repeating unit of the o-specific polymer was determined to be a branched trisaccharide repeating-unit of the structure shown.19968721742
temperature-dependent aminoglycoside resistance in stenotrophomonas (xanthomonas) maltophilia; alterations in protein and lipopolysaccharide with growth temperature.clinical strains of stenotrophomonas (xanthomonas) maltophilia often show large, growth temperature-dependent, variations in their susceptibility (tdvs) to aminoglycoside antibiotics. strains showing more than a fourfold increase in susceptibility between 30 degrees and 37 degrees c (tdvs+ strains; n = 23) were contrasted with those showing lesser variation (tdvs- strains; n = 15) in studies of growth temperature-dependent variation in protein and cell-wall lipopolysaccharide (lps) electrophores ...19968722532
in vitro activity of the tricyclic beta-lactam gv104326.gv104326 is a novel tricyclic beta-lactam (a trinem or, formerly, tribactam). the in vitro activity of gv104326 was compared with those of cefuroxime, cefixime, amoxicillin, amoxicillin-clavulanic acid, cefpirome, and ciprofloxacin. gv104326 had in vitro activity generally similar to that of cefixime against members of the family enterobacteriaceae (mic at which 90% of the isolates are inhibited [mic90], < or = 2 micrograms/ml), with cefuroxime and amoxicillin-clavulanic acid being 8- to 32-fold ...19968723475
in vitro activities of quinolones, beta-lactams, tobramycin, and trimethoprim-sulfamethoxazole against nonfermentative gram-negative bacilli.from 1991 to 1995, 8,975 nonfermentative gram-negative bacilli were isolated from patients at the ohio state university medical center: 71% pseudomonas aeruginosa, 14% stenotrophomonas maltophilia, 7.6% acinetobacter baumannii, and < 2% each of 25 other species. the mics of trovafloxacin (cp-99,219), ciprofloxacin, ofloxacin, ampicillin-sulbactam, piperacillin, piperacillin-tazobactam, ceftazidime, cefoperazone, ceftriaxone, imipenem, tobramycin, and trimethoprim-sulfamethoxazole (tmp-smz) were ...19968726011
the effect of concurrent pseudomonas or xanthomonas exposure on adherence of acanthamoeba castellanii to soft contact lenses.approximately 85% of acanthamoeba-contaminated contact lens systems in asymptomatic patients have concurrent bacterial contamination. pseudomonas aeruginosa and xanthomonas maltophilia are common contact lens contaminants; we investigated the effect of coincubation of acanthamoeba adherence to contact lenses.19968740252
a reappraisal of the diversity and class distribution of aspartate transcarbamoylases in gram-negative bacteria.recently, the subunit composition of class a aspartate transcarbamoylases (atcases) in fluorescent pseudomonads has been clarified. we present evidence that distribution of this type of atcase may be more widespread than at first suspected. bacterial atcases exist in three forms: class a (molecular mass approximately 450-500 kda); class b, typified by escherichia coli atcase (approximately 300 kda); and class c, typified by bacillus subtilis atcase (approximately 100 kda). using gradient gel ele ...19968757751
[nosocomial bacterial infection in comprehensive intensive care unit].to establish the prevalence of nosocomial bacterial infection and changes in resistance in a comprehensive intensive care unit.19968758270
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