Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| cell fingerprinting: an approach to classifying cells according to mass profiles of digests of protein extracts. | we present a statistical framework for classifying cells according to the set of peptide masses obtained by mass spectrometric analysis of digestions of whole cell protein extracts. the digest is separated by high performance liquid chromatography (hplc) coupled directly to a mass spectrometer either by an electrospray interface or by collection to a matrix-assisted laser desorption/ionization target plate. here, the mass to charge ratio, intensity, and hplc retention time of the peptides are me ... | 2001 | 11678037 |
| identification and antimicrobial susceptibility of alcaligenes xylosoxidans isolated from patients with cystic fibrosis. | in the past decade, potential pathogens, including alcaligenes species, have been increasingly recovered from cystic fibrosis (cf) patients. accurate identification of multiply antibiotic-resistant gram-negative bacilli is critical to understanding the epidemiology and clinical implications of emerging pathogens in cf. we examined the frequency of correct identification of alcaligenes spp. by microbiology laboratories affiliated with american cf patient care centers. selective media, an exotoxin ... | 2001 | 11682511 |
| in vitro susceptibility of stenotrophomonas maltophilia to various antimicrobial combinations. | stenotrophomonas maltophilia has emerged as a significant pathogen in compromised patients, causing infections which are difficult to treat. clinical isolates from patients in the tucson area were tested against single and combination antibiotics using three testing methods. ticarcillin/clavulanate, trimethoprim/sulfamethoxazole and trovafloxacin provided comparable inhibitory activity, in vitro. ciprofloxacin, imipenem and ticarcillin were active less often. agreements between disk diffusion an ... | 2001 | 11687317 |
| infection and mucosal injury in cancer treatment. | the oral and gastrointestinal mucosa acts as an important mechanical barrier that prevents local or systemic invasion by microorganisms. cytotoxic chemotherapy-induced mucosal injury (mi) of oral cavity and intestinal epithelium occurs in many patients treated for malignancy. compromise of the mucosal barrier can contribute to local invasion by colonizing microorganisms and, subsequently, to systemic infection. historically, gram-negative bacteremia has been the most problematic bacterial infect ... | 2001 | 11694563 |
| [an update on the susceptibility of stenotrophomonas maltophilia]. | 2001 | 11704768 | |
| smedef multidrug efflux pump contributes to intrinsic multidrug resistance in stenotrophomonas maltophilia. | stenotrophomonas maltophilia is an emerging nosocomial pathogen that displays high-level intrinsic resistance to a variety of structurally unrelated antimicrobial agents. efflux mechanisms are known to contribute to acquired multidrug resistance in this organism, and indeed, one such multidrug efflux system, smedef, was recently identified. still, the importance of smedef to intrinsic antibiotic resistance in s. maltophilia had not yet been determined. reverse transcription-pcr confirmed express ... | 2001 | 11709330 |
| biclonal bacteremia due to stenotrophomonas maltophilia in a cancer patient. | 2001 | 11714054 | |
| management of an older patient with stenotrophomonas maltophilia: is it an emerging pathogen in cystic fibrosis? | 2001 | 11720067 | |
| identification of pandoraea species by 16s ribosomal dna-based pcr assays. | the recently described genus pandoraea contains five named species (pandoraea apista, pandoraea pulmonicola, pandoraea pnomenusa, pandoraea sputorum, and pandoraea norimbergensis) and four unnamed genomospecies. pandoraea spp. have mainly been recovered from the respiratory tracts of cystic fibrosis (cf) patients. accurate genus- and species-level identification by routine clinical microbiology methods is difficult, and differentiation from burkholderia cepacia complex organisms may be especiall ... | 2001 | 11724860 |
| 7alpha-oh epimerisation of bile acids via oxido-reduction with xanthomonas maltophilia. | the microbial 7alpha-oh epimerisation of cholic, chenodeoxycholic, and 12-ketochenodeoxycholic acids (7alpha-oh bile acids) with xanthomonas maltophilia cbs 827.97 to corresponding 7beta-oh derivatives with scarcity of oxygen is described. with normal pressure of oxygen the 7-oh oxidation products are obtained. no biotransformations are achieved in anaerobic conditions. the microbial 7alpha-oh epimerisation is achieved by oxidation of 7-oh function and subsequent reduction. partial purification, ... | 2002 | 11728521 |
| acral necrosis by stenotrophomonas maltophilia. | stenotrophomonas maltophilia (sm) has been considered a nosocomial pathogen. nevertheless, community acquired infection may occur more frequently than usually recognized. | 2001 | 11730046 |
| electronic ventilator temperature sensors as a potential source of respiratory tract colonization with stenotrophomonas maltophilia. | stenotrophomonas maltophilia (s. maltophilia) is an important cause of nosocomial infection among ventilated and immunocompromised patients, and among patients receiving broad-spectrum antimicrobials. we report a cluster of patients in a surgical intensive care unit who were colonized or infected with s. maltophilia. an epidemiological investigation was initiated after surveillance data revealed that eight patients were culture-positive from sputum for s. maltophilia in the preceding month. revi ... | 2001 | 11740879 |
| absolute configuration of 3-hydroxy acids formed by stenotrophomonas maltophilia: application of multidimensional gas chromatography and circular dichroism spectroscopy. | the soil bacterium stenotrophomonas maltophilia was found to transform various long-chain fatty acids selectively into 3-hydroxy fatty acids of shorter chain length. their chiral evaluation was performed by multidimensional gas chromatography (mdgc) on modified cyclodextrin phase comparing the enantiodistribution of 1,3-diol formed without loss of stereochemical information from a representative microbial product with those of synthetic (3rs)- and (3s)-1,3-diols. enantiomeric excesses of 84-98% ... | 2002 | 11748801 |
| a novel metallo-beta-lactamase, mbl1b, produced by the environmental bacterium caulobacter crescentus. | caulobacter crescentus 101123 possesses a gene (mbl1b) encoding a metallo-beta-lactamase with 32% amino acid identity to the l1 metallo-beta-lactamase from stenotrophomonas maltophilia. the gene was cloned into an expression vector and the enzyme, mbl1b, was expressed in escherichia coli. mbl1b was purified. catalytic properties for several antibiotics were determined. the enzyme exhibits michaelis-menten kinetics for imipenem, meropenem and nitrocefin but substrate inhibition kinetics with cefo ... | 2001 | 11749954 |
| burkholderia/stenotrophomonas. | 2001 | 11764736 | |
| weeds as a source of plant growth promoting rhizobacteria in agricultural soils. | the influence of plant growth promoting (pgp) activity of bacterial communities recovered from each of six weed species (barnyard grass (echinochloa crusfalli (l.) beauv.), corn spurrey (spergula arvensis l.), goldenrod (sonchus sp.), italian ryegrass (lolium multiflorum l.), lamb's-quarters (chenopodium album l.), and quack grass (agropyron repens (l.) beauv.)) was examined in relation to the effect it had on the growth of the potato cultivar russet burbank. bacterial species composition and co ... | 2001 | 11766050 |
| distribution and prevalence of antimicrobial resistance among gram-negative isolates in intensive care units (icu) in belgian hospitals between 1996 and 1999. | to assess the distribution and prevalence of resistance rates among gram-negative isolates in belgian intensive care units (icus) between 1996 and 1999. | 2001 | 11770225 |
| comparison of two culture methods for detection of tobramycin-resistant gram-negative organisms in the sputum of patients with cystic fibrosis. | a culture method utilizing quantitative plating on antibiotic-containing media has been proposed as a technique for the detection of tobramycin-resistant organisms that is more sensitive than standard methods. typical sputum culture methods quantitate the relative amounts of each distinct morphotype, followed by antibiotic susceptibility testing of a single colony of each morphotype. sputum specimens from 240 cystic fibrosis patients were homogenized, serially diluted, and processed in parallel ... | 2002 | 11773088 |
| liver abscess caused by stenotrophomonas (xanthomonas) maltophilia in a patient with aids. | 2001 | 11774837 | |
| [analysis of clinical characteristics and drug sensitivity tests of lower respiratory tract infection by xanthomonas maltophilia]. | to analyse the clinical characteristics of lower respiratory tract infection caused by xanthomonas maltophilia and to investigate the antibiotic sensitivity of xanthomonas maltophilia strains. | 1999 | 11775914 |
| comparative in vitro potency of gemifloxacin and fluoroquinolones against recent european clinical isolates from a global surveillance study. | gemifloxacin, a new fluoroquinolone with enhanced activity against gram-positive aerobes, was compared to ciprofloxacin, levofloxacin and ofloxacin against 21,464 recent isolates from 16 european countries. gemifloxacin was the most potent fluoroquinolone against streptococci including penicillin-, macrolide- and ciprofloxacin-resistant streptococcus pneumoniae, staphylococcus aureus, coagulase-negative staphylococci, acinetobacter spp., haemophilus spp. and moraxella catarrhalis. this drug was ... | 2001 | 11783700 |
| smec, an outer membrane multidrug efflux protein of stenotrophomonas maltophilia. | a homologue of the mexab-oprm multidrug efflux operon of pseudomonas aeruginosa, smeabc, was cloned from stenotrophomonas maltophilia by using, as a probe, a pcr product amplified from this organism with primers based on the mexb sequence. the smeabc genes were hyperexpressed in a mutant strain displaying resistance to several antimicrobials, including aminoglycosides, beta-lactams, and fluoroquinolones. deletions in smec but not smeb compromised this resistance, suggesting that smec contributed ... | 2002 | 11796339 |
| the major extracellular protease of the nosocomial pathogen stenotrophomonas maltophilia: characterization of the protein and molecular cloning of the gene. | stenotrophomonas maltophilia is increasingly emerging as a multiresistant pathogen in the hospital environment. in immunosuppressed patients, these bacteria may cause severe infections associated with tissue lesions such as pulmonary hemorrhage. this suggests proteolysis as a possible pathogenic mechanism in these infections. this study describes a protease with broad specificity secreted by s. maltophilia. the gene, termed stmpr1, codes for a 63-kda precursor that is processed to the mature pro ... | 2002 | 11796713 |
| microflora investigation experiment. | many microorganisms were isolated from condensed water, wiping and scratching of cabin wall and air sampler in russian space station mir by russian astronauts lazutkin et al. in february 1997 as part of nasda "first mir utilization space experiment (jmir)". for example, there were about 2 x 10(6) cells/ml in condensed water sample no. 1 isolated from the transfer-docking compartment of crystal module. we tried the colony isolation, pure culturing and identification from these sampled microorgani ... | 2001 | 11799257 |
| crystallization and preliminary x-ray data of the recombinant peptide amidase from stenotrophomonas maltophilia. | the peptide amidase from stenotrophomonas maltophilia selectively hydrolyses the c-terminal amide bond in peptide amides. crystals have been obtained by sitting-drop vapour diffusion from solution containing polyethylene glycol (peg) 6000, hepes ph 7.5, glycerine and sodium azide (nan(3)). the crystals belong to the monoclinic space group p2(1), with unit-cell parameters a = 74.18, b = 62.60, c = 101.91 a, beta = 90 degrees. x-ray data from these crystals diffracted at the european synchrotron r ... | 2002 | 11807268 |
| bacteremia due to multiresistant gram-negative bacilli in neutropenic cancer patients: a case-controlled study. | the aim of this study was to assess whether multiresistant gram-negative bacteremias (mrgnb) were associated with specific risk factors for higher mortality than sensitive gram-negative bacteremias. two groups of subjects: (51 cases and 102 controls) were matched for sex, age, underlying disease, and neutropenia. there were no significant differences in the incidence of cytotoxic chemotherapy administered, vascular catheter insertion, catheter as source of bacteremia, and etiology of bacteremia. ... | 1999 | 11810513 |
| susceptibility to fluoroquinolones among commonly isolated gram-negative bacilli in 2000: trust and tsn data for the united states. tracking resistance in the united states today. the surveillance network. | from january to may 2000, as part of the tracking resistance in the united states today (trust) surveillance initiative, clinical isolates of enterobacteriaceae (n=2519) and non-fermentative gram-negatives (n=580) were prospectively collected from 26 hospital laboratories across the united states. isolates were tested for susceptibility to three fluoroquinolones (ciprofloxacin, levofloxacin, gatifloxacin) and seven other agents. in addition, data for the same period were collected from the surve ... | 2002 | 11814764 |
| differential regulation of l1 and l2 beta-lactamase expression in stenotrophomonas maltophilia. | it has been reported that stenotrophomonas maltophilia l1 and l2 beta-lactamase expression is coordinated. we have isolated s. maltophilia mutants where (i) l1 is constitutively hyper-expressed and l2 is inducible; (ii) l2 is hyper-expressed and l1 is inducible; and (iii) l1 and l2 are constitutively hyper-expressed. the frequency of isolating type 1 and 2 mutants is c. 10(-7), indicating that promoter mutations are probably not involved and providing strong evidence that l1 and l2 expression is ... | 2002 | 11815585 |
| severe cellulitis/myositis caused by stenotrophomonas maltophilia. | to present a case of cellulitis/myositis due to stenotrophomonas maltophilia in the absence of trauma and to discuss a potentially novel treatment option. | 2002 | 11816260 |
| assessment of chitin decomposer diversity within an upland grassland. | the breakdown of chitin within an acidic upland grassland was studied. the aim was to provide a molecular characterisation of microorganisms involved in chitin degradation in the soil using soil microcosms and buried litter bags containing chitin. the investigation involved an examination of the effects of liming on the microbial communities within the soil and their chitinolytic activity. microcosm experiments were designed to study the influence of lime and chitin enrichment on the grassland s ... | 2001 | 11816968 |
| l-glucitol catabolism in stenotrophomonas maltophilia ac. | the carbohydrate catabolism of the bacterium stenotrophomonas maltophilia ac (previously named pseudomonas sp. strain ac), which is known to convert the unnatural polyol l-glucitol to d-sorbose during growth on the former as the sole source of carbon and energy, was studied in detail. all enzymes operating in a pathway that channels l-glucitol via d-sorbose into compounds of the intermediary metabolism were demonstrated, and for some prominent reactions the products of conversion were identified ... | 2002 | 11823194 |
| 16s ribosomal dna-based analysis of bacterial diversity in purified water used in pharmaceutical manufacturing processes by pcr and denaturing gradient gel electrophoresis. | the bacterial community in partially purified water, which is prepared by ion exchange from tap water and is used in pharmaceutical manufacturing processes, was analyzed by denaturing gradient gel electrophoresis (dgge). 16s ribosomal dna fragments, including v6, -7, and -8 regions, were amplified with universal primers and analyzed by dgge. the bacterial diversity in purified water determined by pcr-dgge banding patterns was significantly lower than that of other aquatic environments. the bacte ... | 2002 | 11823209 |
| multiple mechanisms of antimicrobial resistance in pseudomonas aeruginosa: our worst nightmare? | pseudomonas aeruginosa carries multiresistance plasmids less often than does klebsiella pneumoniae, develops mutational resistance to cephalosporins less readily than enterobacter species, and has less inherent resistance than stenotrophomonas maltophilia. what nevertheless makes p. aeruginosa uniquely problematic is a combination of the following: the species' inherent resistance to many drug classes; its ability to acquire resistance, via mutations, to all relevant treatments; its high and inc ... | 2002 | 11823954 |
| post-traumatic endophthalmitis caused by xanthomonas maltophilia. | 2001 | 11827012 | |
| microbial degradation of polyethers. | this paper summarizes studies on microbial degradation of polyethers. polyethers are aerobically metabolized through common mechanisms (oxidation of terminal alcohol groups followed by terminal ether cleavage), well-characterized examples being found with polyethylene glycol (peg). first the polymer is oxidized to carboxylated peg by alcohol and aldehyde dehydrogenases and then the terminal ether bond is cleaved to yield the depolymerized peg by one glycol unit. most probably peg is anaerobicall ... | 2002 | 11831473 |
| [stenotrophomonas maltophilia as a cause of hospital infections--typing of clinical isolates using pulsed field gel electrophoresis] ]. | stenotrophomonas maltophilia (s. maltophilia) has been recognised now as an important cause of hospital infections. as s. maltophilia is resistant to many antibiotics, attempts have been made to identify the sources of s. maltophilia infection and route of its transmission. from july till october 1998, 22 isolates of s. maltophilia were obtained from 20 patients hospitalised at eight different wards. strain typing was performed by macrorestriction analysis of chromosomal dna by use of pfge (xbai ... | 2001 | 11845578 |
| functional control of the binuclear metal site in the metallo-beta-lactamase-like fold by subtle amino acid replacements. | at present there are three protein families that share a common structural domain, the alphabeta/betaalpha fold of class b beta-lactamases: zinc beta-lactamases, glyoxalases ii, and a-type flavoproteins. a detailed inspection of their superimposed structures was undertaken and showed that although these proteins contain binuclear metal sites in spatially equivalent positions, there are some subtle differences within the first ligand sphere that determine a distinct composition of metals. althoug ... | 2002 | 11847294 |
| topoisomerase ii and iv quinolone resistance-determining regions in stenotrophomonas maltophilia clinical isolates with different levels of quinolone susceptibility. | the quinolone resistance-determining regions (qrdrs) of topoisomerase ii and iv genes from stenotrophomonas maltophilia atcc 13637 were sequenced and compared with the corresponding regions of 32 unrelated s. maltophilia clinical strains for which ciprofloxacin mics ranged from 0.1 to 64 microg/ml. gyra (leu-55 to gln-155, escherichia coli numbering), gyrb (met-391 to phe-513), parc (ile-34 to arg-124), and pare (leu-396 to leu-567) fragments from strain atcc 13637 showed high degrees of identit ... | 2002 | 11850246 |
| in vitro activities of tigecycline (gar-936) against recently isolated clinical bacteria in spain. | the antimicrobial activities of tigecycline (gar-936) were compared with those of other agents against 1,087 strains recently isolated in 12 spanish medical centers. tigecycline showed activity against a wide spectrum of aerobic and anaerobic bacteria, including strains such as methicillin-resistant staphylococcus aureus, coagulase-negative staphylococci, penicillin-resistant streptococcus pneumoniae, enterococcus faecium, acinetobacter baumannii, and stenotrophomonas maltophilia. | 2002 | 11850282 |
| antimicrobial activity evaluations of gatifloxacin, a new fluoroquinolone: contemporary pathogen results from a global antimicrobial resistance surveillance program (sentry, 1997). | objective: to investigate the in vitro potency and spectrum of activity of gatifloxacin and five comparator fluoroquinolones tested against over 23 000 clinical isolates from diverse geographic and clinical sources in the americas. methods: gram-negative, gram-positive and fastidious bacterial isolates were tested against gatifloxacin, ciprofloxacin, levofloxacin, ofloxacin, sparfloxacin and trovafloxacin using broth microdilution methods recommended by the national committee for clinical labora ... | 1999 | 11851706 |
| evaluation of the id 32e for the identification of gram-negative glucose-fermenting and glucose-non-fermenting bacilli. | objective: to evaluate the id 32e bacterial identification system for accuracy in the identification of members of the family enterobacteriaceae, pseudomonas aeruginosa, stenotrophomonas maltophilia, and acinetobacter baumannii/iwoffii. methods: stock cultures of 497 enterobacteriaceae and 27 commonly encountered non-enteric gram-negative rods were tested in the id 32e system. for each isolate, the resulting 11-digit profile number was converted to an identification using the apilab plus softwar ... | 1999 | 11856267 |
| in vitro activity of clinafloxacin in comparison with other quinolones against stenotrophomonas maltophilia clinical isolates in the presence and absence of reserpine. | a total of 33 stenotrophomonas maltophilia clinical isolates were tested for their susceptibility to clinafloxacin in comparison with ciprofloxacin, levofloxacin, moxifloxacin, nalidixic acid, norfloxacin, sparfloxacin and trovafloxacin. the mic(50) and mic(90) were as follows: ciprofloxacin 4 and 64 microg/ml; clinafoxacin 0.5 and 4 microg/ml; levofloxacin 2 and 32 microg/ml; moxifloxacin 1 and 8 microg/ml; nalidixic acid 8 and 128 microg/ml; norfloxacin 64 and 256 microg/ml; sparfloxacin 1 and ... | 2002 | 11858908 |
| stenotrophomonas maltophilia endocarditis: a systematic review. | the disease characteristics, management, and outcome of stenotrophomonas maltophilia endocarditis were evaluated by examining the reports on the subject identified through a comprehensive literature search. twenty-three (17 male) cases of s.. maltophilia endocarditis were identified. mean age was 41 +/- 15 years. all patients presented with fever. prosthetic valves were involved in 12 (52%) cases. among native valves, the aortic valve was most frequently involved (50%), followed by the tricuspid ... | 2002 | 11863309 |
| high frequency of antibiotic resistance among gram-negative isolates in intensive care units at 10 swedish hospitals. | objective: to investigate the resistance rates among gram-negative isolates in swedish intensive care units (icus). methods: during 1994-95, members of the swedish study group collected, on clinical indication, 502 consecutive initial isolates of gram-negative bacteria from patients admitted to icus at 10 swedish hospitals and performed minimal inhibitory concentration (mic) determinations with the etest. breakpoints were defined according to the criteria of the swedish reference group for antib ... | 1997 | 11864106 |
| cq-397 and cq-414: antimicrobial activity and spectrum of two fluoroquinolone---cephalosporin, dual-action compounds with carboxamido bonds. | objective: to evaluate the potential spectrum of activity of two novel dual-action compounds with carboxamido bonds (cq-397 and cq-414; laboratorios aranda, san rafael, mexico) against human pathogens. methods: approximately 800 gram-positive and gram-negative aerobic clinical bacteria were tested in vitro using the mueller-hinton broth microdilution method of the national committee of clinical laboratory standards. results: cq-397 (cefamandole+enrofloxacin) and cq-414 (cefamandole+norfloxacin) ... | 1997 | 11864130 |
| the comparative in vitro activity of fk-037 (cefoselis), a new broad-spectrum cephalosporin. | fk-037, a new parenteral cephalosporin, was active against clinical isolates of both gram-positive and gram-negative aerobic bacteria. the activity of fk---037 was similar to that of cefpirome and cefepime and generally superior to that of ceftazidime. methicillin-resistant staphylococci were less susceptible than methicillin-sensitive isolates but most remained within the sensitive range. all streptococci and enterobacteriaceae were sensitive as were the majority of isolates of acinetobacter sp ... | 1995 | 11866715 |
| comparative in vitro activity of apalcillin alone and combined with ro 48-1220, a novel penam beta-lactamase inhibitor. | objective: the in vitro activity of apalcillin plus ro 48-1220, a novel penam sulfone beta-lactamase inhibitor, was compared with apalcillin alone, piperacillin/tazobactam, ticarcillin/clavulanic acid, amoxicillin/clavulanic acid, imipenem, ceftazidime and cefepime. methods: agar dilution and broth microdilution testing of 854 bacterial strains, subcultured from frozen stocks incubated for 24 h in 5% carbon dioxide, was carried out to determine the minimum bactericidal (mbc) and minimum inhibito ... | 1995 | 11866735 |
| characterization of a chitinase gene from stenotrophomonas maltophilia strain 34s1 and its involvement in biological control. | a chitinase gene was cloned on a 2.8-kb dna fragment from stenotrophomonas maltophilia strain 34s1 by heterologous expression in burkholderia cepacia. sequence analysis of this fragment identified an open reading frame encoding a deduced protein of 700 amino acids. removal of the signal peptide sequence resulted in a predicted protein that was 68 kda in size. analysis of the sequence indicated that the chitinase contained a catalytic domain belonging to family 18 of glycosyl hydrolases. three pu ... | 2002 | 11872449 |
| microscale biosensor for measurement of volatile fatty acids in anoxic environments. | a microscale biosensor for acetate, propionate, isobutyrate, and lactate is described. the sensor is based on the bacterial respiration of low-molecular-weight, negatively charged species with a concomitant reduction of no(-)(3) to n(2)o. a culture of denitrifying bacteria deficient in n(2)o reductase was immobilized in front of the tip of an electrochemical n(2)o microsensor. the bacteria were separated from the outside environment by an ion-permeable membrane and supplied with nutrients (excep ... | 2002 | 11872469 |
| microbial populations of an upflow anaerobic sludge blanket reactor treating wastewater from a gelatin industry. | the microbial populations of an upflow anaerobic sludge blanket reactor, used for treating wastewater from the gelatin industry, were studied by microbiological methods and phase-contrast and electron microscopy. microscopy examination of the sludge showed a complex mixture of various rod-shaped and coccoid bacterial pluslong filaments and verymobile curved rods. in addition free-living anaerobic ciliates and flagellates were also observed. the trophic group population observed in decreasing ord ... | 2001 | 11873883 |
| probing substrate binding to metallo-beta-lactamase l1 from stenotrophomonas maltophilia by using site-directed mutagenesis. | the metallo-beta-lactamases are zn(ii)-containing enzymes that hydrolyze the beta-lactam bond in penicillins, cephalosporins, and carbapenems and are involved in bacterial antibiotic resistance. there are at least 20 distinct organisms that produce a metallo-beta-lactamase, and these enzymes have been extensively studied using x-ray crystallographic, computational, kinetic, and inhibition studies; however, much is still unknown about how substrates bind and the catalytic mechanism. in an effort ... | 2002 | 11876827 |
| open lung biopsy in pediatric bone marrow transplant patients. | the aim of this study was to evaluate the clinical benefits of open lung biopsy in the diagnosis and treatment of pulmonary infiltrates in children who have undergone bone marrow transplantation. | 2002 | 11877664 |
| [effectiveness of contact isolation in the control of multiresistant bacteria in an intensive care service]. | to describe the frequency, characteristics and progression of critically ill patients admitted to the icu, for whom isolation is indicated due to detection of multiresistant pathogenic bacteria, and to study the effectiveness of precautionary measures to avoid dissemination of these microorganisms. | 2002 | 11886673 |
| pulmonary infections in patients with cystic fibrosis. | cystic fibrosis (cf) is an autosomal-recessive disorder and affects about 60,000 people worldwide. the cf gene, the cystic fibrosis transmembrane conductance regulator (cftr), was found in 1989 and over 800 mutations have been sequenced. although our understanding of the pathophysiology of cf has increased, pulmonary infections remain the major cause of morbidity and mortality. during the first decade of life, staphylococcus aureus and nontypable haemophilus influenza are most common, but pseudo ... | 2002 | 11891518 |
| broad-range bacterial detection and the analysis of unexplained death and critical illness. | broad-range rdna polymerase chain reaction (pcr) provides an alternative, cultivation-independent approach for identifying pathogens. in 1995, the centers for disease control and prevention initiated population-based surveillance for unexplained life-threatening infections (unexplained death and critical illness project [unex]). to address the causes of unex cases, we examined 59 specimens from 46 cases by using broad-range bacterial 16s rdna pcr and phylogenetic analysis of amplified sequences. ... | 2002 | 11897072 |
| synergistic activities of macrolide antibiotics against pseudomonas aeruginosa, burkholderia cepacia, stenotrophomonas maltophilia, and alcaligenes xylosoxidans isolated from patients with cystic fibrosis. | azithromycin and clarithromycin were paired with other antibiotics to test synergistic activity against 300 multidrug-resistant pathogens isolated from cystic fibrosis (cf) patients. clarithromycin-tobramycin was most active against pseudomonas aeruginosa and inhibited 58% of strains. azithromycin-trimethoprim-sulfamethoxazole, azithromycin-ceftazidime, and azithromycin-doxycycline or azithromycin-trimethoprim-sulfamethoxazole inhibited 40, 20, and 22% of stenotrophomonas maltophilia, burkholder ... | 2002 | 11897598 |
| effect of an efflux pump inhibitor on the mic of nalidixic acid for acinetobacter baumannii and stenotrophomonas maltophilia clinical isolates. | 2002 | 11909848 | |
| analysis of bacteria contaminating ultrapure water in industrial systems. | bacterial populations inhabiting ultrapure water (upw) systems were investigated. the analyzed upw systems included pilot scale, bench scale, and full size upw plants employed in the semiconductor and other industries. bacteria present in the polishing loop of the upw systems were enumerated by both plate counts and epifluorescence microscopy. assessment of bacterial presence in upw by epifluorescence microscopy (cyanotolyl tetrazolium chloride [ctc] and dapi [4',6'-diamidino-2-phenylindole] sta ... | 2002 | 11916667 |
| recent developments in carbapenems. | carbapenems are beta-lactam antibiotics characterised by the presence of a beta-lactam ring with a carbon instead of sulfone in the 4-position of the thyazolidinic moiety. the first carbapenem to be utilised in therapy was imipenem, the n-formimidoyl derivative of thienamycin. imipenem is coadministered with cilastatin, an inhibitor of human renal dehydropeptidase i, as imipenem is hydrolysed by this enzyme. meropenem was the first carbapenem with a 1-beta-methyl group and 2-thio pyrrolidinyl mo ... | 2002 | 11922861 |
| rapid direct antibiotic susceptibility testing in endophthalmitis. | to compare a new rapid antibiotic susceptibility test (rast) to the conventional method in patients with endophthalmitis. | 2002 | 11927425 |
| sinusitis due to stenotrophomonas maltophilia. | this is the first report of a patient diagnosed with sinusitis due to stenotrophomonas maltophilia. despite the organism being resistant to trimethoprim-sulfamethoxazole, the infection was cured by drainage and treatment with intravenous piperacillin, followed by oral ciprofloxacin. | 2002 | 11928848 |
| phylogenetic analysis of xanthomonas species based upon 16s-23s rdna intergenic spacer sequences. | phylogenetic relationships of 17 species of xanthomonas were assessed based on analysis of 16s-23s rdna intergenic spacer (its) sequences; a higher level of resolution was obtained than that revealed by 16s rdna analysis. its sequences varied in size from 492 to 578 nt within the genus and the similarity among sequences ranged from 63 to 99%. major differences were found for the hyacinthi group, which included xanthomonas albilineans, xanthomonas hyacinthi, xanthomonas sacchari, xanthomonas thei ... | 2002 | 11931143 |
| thermomonas haemolytica gen. nov., sp. nov., a gamma-proteobacterium from kaolin slurry. | four aerobic, gram-negative bacterial strains isolated from kaolin slurry used in the production of paper were subjected to a polyphasic analysis and characterization to determine their taxonomic position. analysis of the 16s rdna sequences of the four strains revealed that they represent a new lineage within the gamma-proteobacteria, related to the genera xanthomonas, pseudoxanthomonas, stenotrophomonas, luteimonas, xylella and rhodanobacter. analysis of the quinone system, the polyamines, the ... | 2002 | 11931159 |
| stenotrophomonas acidaminiphila sp. nov., a strictly aerobic bacterium isolated from an upflow anaerobic sludge blanket (uasb) reactor. | two of several strictly aerobic, mesophilic bacteria isolated from a lab-scale upflow anaerobic sludge blanket (uasb) reactor treating a petrochemical wastewater, strains amx 17 and amx 19t, were subjected to detailed taxonomic study. cells were gram-negative, motile, non-sporulating, straight to curved rods with a polar flagellum. the isolates exhibited phenotypic traits of members of the genus stenotrophomonas, including cellular fatty acid composition and the limited range of substrates that ... | 2002 | 11931169 |
| the flavonoid galangin inhibits the l1 metallo-beta-lactamase from stenotrophomonas maltophilia. | the flavonoid galangin inhibits the partially purified metallo-beta-lactamase from stenotrophomonas maltophilia. the effect was not reversed by the addition of zncl(2) suggesting that the inhibitory effect is not related to metal chelation. the flavonoid quercetin also has some inhibitory effect against the enzyme. using the crystal structure of the enzyme, a molecular modelling study predicts a possible orientation of galangin at the active site of the enzyme. | 2002 | 11934488 |
| new synthetic siderophores and their beta-lactam conjugates based on diamino acids and dipeptides. | linking of siderophores to antibiotics improves the penetration and therefore increases the antibacterial activity of the antibiotics. we synthesized the acylated catecholates and hydroxamates as siderophore components for antibiotic conjugates to reduce side effects of unprotected catecholate and hydroxamate moieties. in this paper, we report on bis- and tris-catecholates and mixed catecholate hydroxamates based on diamino acids or dipeptides. these compounds were active as siderophores in a gr ... | 2002 | 11937324 |
| characterization of monomeric l1 metallo-beta -lactamase and the role of the n-terminal extension in negative cooperativity and antibiotic hydrolysis. | the l1 metallo-beta-lactamase from stenotrophomonas maltophilia is unique among this class of enzymes because it is tetrameric. previous work predicted that the two regions of important intersubunit interaction were the residue met-140 and the n-terminal extensions of each subunit. the n-terminal extension was also implicated in beta-lactam binding. mutation of methionine 140 to aspartic acid results in a monomeric l1 beta-lactamase with a greatly altered substrate specificity profile. a 20-amin ... | 2002 | 11940588 |
| selective (r)-3-hydroxylation of fa by stenotrophomonas maltophilia. | soil samples were screened for microorganisms selectively transforming fa. one of the isolated strains was identified as the bacterium stenotrophomonas maltophilia by its phenotypic features and genotypic characterization by sequencing the ribosomal rna gene. using linoleic acid as substrate resulted in the formation of two major compounds. after liquid chromatographic isolation and separation, their structures were elucidated by hplc-tandem ms, gc-ms, and nmr techniques to be 3-hydroxy-z6-dodec ... | 2002 | 11942484 |
| two episodes of stenotrophomonas maltophilia endocarditis of prosthetic mitral valve: report of a case and review of the literature. | stenotrophomonas maltophilia (previously named xanthomonas maltophilia) is an aerobic, non-fermentive, gram-negative bacillus that is wide spread in the environment. it was considered to be an organism with limited pathogenic potential, which was rarely capable of causing diseases in human other than those who were in debilitated or immunocompromised state. more recent studies have established that stenotrophomonas maltophilia can behave as a true pathogen. endocarditis due to this organism is r ... | 2002 | 11961315 |
| [monitoring the incidence of multidrug-resistant bacteria in microbiology laboratories using bacterio software]. | since 1997, the microbiology laboratory of the hospital center in lens has monitored hospital ecology and epidemiology to provide rapid information that help to adapt probabilistic antibiotherapy and to estimate the impact of hygienic preventive measures on the incidence of multidrug-resistant bacteria (mrb). | 2002 | 11963375 |
| substrate-activated zinc binding of metallo-beta -lactamases: physiological importance of mononuclear enzymes. | we have investigated the influence of substrate binding on the zinc ion affinity of representatives from the three metallo-beta-lactamase subclasses, b1 (bcii from bacillus cereus and blab from chryseobacterium meningosepticum), b2 (cpha from aeromonas hydrophila), and b3 (l1 from stenotrophomonas maltophilia). by competition experiments with metal-free apoenzymes and chromophoric zinc chelators or edta, we determined the dissociation constants in the absence and presence of substrates. for the ... | 2002 | 11967267 |
| stenotrophomonas maltophilia bacteraemia in two patients with cystic fibrosis associated with totally implantable venous access devices. | 2002 | 11972423 | |
| [post-marketing surveillance of antibacterial activities of cefozopran against various clinical isolates--ii. gram-negative bacteria]. | as a post-marketing surveillance, the in vitro antibacterial activities of cefozopran (czop), an agent of cephems, against various clinical isolates were yearly evaluated and compared with those of other cephems, oxacephems, penicillins, monobactams, and carbapenems. changes in czop susceptibility for the bacteria were also evaluated with the bacterial resistance ratio calculated with the breakpoint mic. twenty-five species (3,362 strains) of gram-negative bacteria were isolated from the clinica ... | 2002 | 11977920 |
| molecular epidemiology of stenotrophomonas maltophilia isolated from cystic fibrosis patients. | 2002 | 11980986 | |
| [nosocomial infection due to stenotrophomona maltophilia in surgically treated patients]. | 2002 | 11996709 | |
| clinical epidemiology of stenotrophomonas maltophilia colonization and infection: a multicenter study. | 2002 | 11997719 | |
| degradation characteristics of toluene, benzene, ethylbenzene, and xylene by stenotrophomonas maltophilia t3-c. | stenotrophomonas maltophilia t3-c, isolated from a biofilter for the removal of benzene, toluene, ethylbenzene, and xylene (btex), could grow in a mineral salt medium containing toluene, benzene, or ethylbenzene as the sole source of carbon. the effect of environmental factors such as initial toluene mass, medium ph, and temperature on the degradation rate of toluene was investigated. the cosubstrate interactions in the btex mixture by the isolate were also studied. within the range of initial t ... | 2002 | 12002185 |
| hospital-acquired infections following the 1999 marmara earthquake. | in this study, medical records of all casualties admitted to our hospital following the marmara earthquake, which struck northwest turkey and resulted in the destruction of several towns in the marmara region, were evaluated retrospectively. the time buried under the rubble, demographic data, type of medical and surgical therapies performed, type of injury and data on infection were analysed. between 17 august and 25 september 1999, 630 trauma victims were received at our hospital and 532 (84%) ... | 2002 | 12009820 |
| [synthesis of signaling n-acyl-homoserine-lactones participating in quorum sensing in rhizosphere and soil bacteria pseudomonas and xanthomonas]. | signaling molecules assigned to n-acyl-homoserine-lactones (ahl) serve as autoinducers for the genes controlling the quorum sensing regulatory system. in many gram-negative bacteria, ahl are the key factors responsible for density-dependent regulation of exoenzyme and secondary metabolite production; they also participate in interaction between bacteria and higher organisms. the soil and rhisosphere bacteria pseudomonas and xanthomonas from different geographical zones of russia and the former u ... | 2002 | 12018176 |
| fulminant stenotrophomonas maltophilia soft tissue infection in immunocompromised patients: an outbreak transmitted via tap water. | soft tissue infection caused by stenotrophomonas maltophilia is uncommon, but nosocomial infections had been reported. we describe herein 2 young female patients, with severe neutropenia, on broad spectrum antimicrobial agents for neutropenic fever, with hickman-type central venous catheter, who developed mucocutaneous and soft tissue infections with rapidly progressive and devastating course. cultures from the skin of both patients and from blood of one of them grew s. maltophilia. both patient ... | 2002 | 12018671 |
| cau-1, a subclass b3 metallo-beta-lactamase of low substrate affinity encoded by an ortholog present in the caulobacter crescentus chromosome. | the sequenced chromosome of caulobacter crescentus cb15 encodes a hypothetical protein that exhibits significant similarity (30 to 35% identical residues) to metallo-beta-lactamases of subclass b3. an allelic variant of this gene (divergent by 3% of its nucleotides) was cloned in escherichia coli from c. crescentus type strain dsm4727. expression studies confirmed the metallo-beta-lactamase activity of its product, cau-1. the enzyme produced in e. coli was purified by two ion-exchange chromatogr ... | 2002 | 12019096 |
| identification of a series of tricyclic natural products as potent broad-spectrum inhibitors of metallo-beta-lactamases. | this work describes the discovery and characterization of a novel series of tricyclic natural product-derived metallo-beta-lactamase inhibitors. natural product screening of the bacillus cereus ii enzyme identified an extract from a strain of chaetomium funicola with inhibitory activity against metallo-beta-lactamases. sb236050, sb238569, and sb236049 were successfully extracted and purified from this extract. the most active of these compounds was sb238569, which possessed k(i) values of 79, 17 ... | 2002 | 12019104 |
| gene cloning, overexpression and biochemical characterization of the peptide amidase from stenotrophomonas maltophilia. | the peptide amidase (pam) from the gram-negative bacterium stenotrophomonas maltophilia catalyzes predominantly the hydrolysis of the c-terminal amide bond in peptide amides. its gene ( pam) was isolated by southern hybridization using a dna probe derived from the known n-terminal amino acid sequence. pam is a member of the amidase signature family and was identified as a periplasmic protein by an n-terminal signal peptide found in the gene. the processed protein consists of 503 amino acids with ... | 2002 | 12021798 |
| septicaemia due to glucose non-fermenting, gram-negative bacilli other than pseudomonas aeruginosa in children. | bloodstream infections due to non-fermenting gram-negative bacilli other than pseudomonas aeruginosa (nf-gnb) are uncommon in children but their incidence is reported to be increasing. the aim of this study was to determine the characteristics of such infections in children in a london teaching hospital. all paediatric patients with positive nf-gnb blood cultures and clinical evidence of sepsis between july 1995 and june 2000 were included in the study. a total of 10278 blood cultures was perfor ... | 2002 | 12022303 |
| two cases of stenotrophomonas maltophilia endophthalmitis. | two cases of stenotrophomonas maltophilia endophthalmitis, a rare cause of postoperative endophthalmitis, are reported in this study. both of the patients underwent vitreus tap and intravitreal antibiotic injection. the therapy was augmented with systemic and subconjunctival injections. the response to this therapy protocol was excellent and the final visual acuity in both of the patients was 10/10. the results are discussed and the literature, which consists of only 8 reported cases of stenotro ... | 2002 | 12027111 |
| purification and characterization of an alkaline serine endopeptidase from a feather-degrading xanthomonas maltophilia strain. | a keratinolytic xanthomonas maltophilia strain (poa-1), cultured on feather meal broth, using keratin as its sole source of carbon and nitrogen, secretes several extracellular peptidases. the major serine peptidase was purified to homogeneity by a five-step procedure. its purity was evaluated by capillary zone electrophoresis. this enzyme has a molecular mass of 36 kda, an optimum ph of 9.0, and an optimum temperature of 60 degrees c. the inhibitory profile using protease inhibitors shows that t ... | 2002 | 12030707 |
| analysis of endophytic bacterial communities of potato by plating and denaturing gradient gel electrophoresis (dgge) of 16s rdna based pcr fragments. | the diversity of endophytic bacterial populations of potato (solanum tuberosum cv desirée) was assessed using a combination of dilution plating of plant macerates followed by isolation and characterization of isolates, and direct pcr-dgge on the basis of dna extracted from plants. the culturable endophytic bacterial communities detected in potato stem bases as well as in roots were in most cases on the order 103 to 105 cfu g?1 of fresh plant tissue. dilution plating revealed that a range of bact ... | 2001 | 12032611 |
| attributable mortality of stenotrophomonas maltophilia bacteremia. | a systematic evaluation of the attributable mortality of stenotrophomonas maltophilia bacteremia was undertaken in a matched, retrospective, case-control study. we determined the attributable mortality rate (26.7%) and mortality risk ratio (an 8-fold increase) of s. maltophilia bacteremia. the attributable mortality rate for s. maltophilia bacteremia is similar to the attributable mortality rate for other nosocomial bloodstream infections. | 2002 | 12032905 |
| heterogeneity of dehydrogenases of stenotrophomonas maltophilia showing dye-linked activity with polypropylene glycols. | distinct enzyme activities were found in extracts from stenotrophomonas maltophilia showing dye-linked oxidation of polypropylene glycols. the activities were induced when polypropylene glycols served as sole carbon and energy sources for the bacterium. in the logarithmic phase, most of the enzyme activities (88%) were found in the cytoplasm. in the stationary phase, more than half of the activities (54%) were found on the membrane, but significant activities were also distributed in the peripla ... | 2002 | 12036044 |
| prevalent bacterial species and novel phylotypes in advanced noma lesions. | the purpose of this study was to determine the bacterial diversity in advanced noma lesions using culture-independent molecular methods. 16s ribosomal dna bacterial genes from dna isolated from advanced noma lesions of four nigerian children were pcr amplified with universally conserved primers and spirochetal selective primers and cloned into escherichia coli. partial 16s rrna sequences of approximately 500 bases from 212 cloned inserts were used initially to determine species identity or close ... | 2002 | 12037085 |
| characterization of cholylglycine hydrolase from a bile-adapted strain of xanthomonas maltophilia and its application for quantitative hydrolysis of conjugated bile salts. | purified bile salt hydrolase from bile-adapted xanthomonas maltophilia displays michaelis-menten kinetics on cholylglycine and cholyltaurine and hydrolyzes bile salts also in crude bovine bile. the protein is a dimer and is resistant to proteinases and to heating at 55 to 60 degrees c for up to 60 min, in agreement with calorimetric data. | 2002 | 12039776 |
| microbiota of periapical lesions refractory to endodontic therapy. | the periapical microbiota of 36 teeth with refractory apical periodontitis was investigated. none of the teeth had responded to conventional endodontic or long-term (> 6 months), calcium-hydroxide treatment. eight patients had received antibiotics systemically. after anaerobic culture, a total of 148 microbial strains were detected among 67 microbial species. one of the 36 lesions was culture-negative. approximately half (51.0%) of the bacterial strains were anaerobic. gram-positive species cons ... | 2002 | 12043869 |
| factors affecting the incidence of stenotrophomonas maltophilia isolation in cystic fibrosis. | to identify factors predisposing cystic fibrosis (cf) patients to stenotrophomonas maltophilia infection and to determine whether coinfection with s maltophilia affects the clinical response to therapy with tobramycin solution for inhalation (tsi), 300 mg bid. | 2002 | 12065335 |
| stenotrophomonas maltophilia meningitis, bacteremia and respiratory infection. | stenotrophomonas maltophilia is an increasingly recognized cause of nosocomial infection of special interest because of its resistance to multiple antimicrobial agents. we report a case of generalized infection by s. maltophilia, including meningitis, bacteremia and respiratory tract infection, in a patient who had undergone multiple neurosurgical procedures and who was treated with trimethoprim-sulphamethoxazole. | 2002 | 12069028 |
| [yearly changes in antibacterial activities of cefozopran against various clinical isolates between 1996 and 2000--ii. gram-negative bacteria]. | the in vitro antibacterial activities of cefozopran (czop), an agent of cephems, against various clinical isolates obtained between 1996 and 2000 were yearly evaluated and compared with those of other cephems, oxacephems, and carbapenems. thirty-two species 2,697 strains of gram-negative bacteria were isolated from the clinical materials annually collected from january to december, and consisted of moraxella subgenus branhamella catarrhalis (n = 125), escherichia coli (n = 250), citrobacter freu ... | 2002 | 12071094 |
| metabolite repression inhibits degradation of benzo[a]pyrene and dibenz[a,h]anthracene by stenotrophomonas maltophilia vun 10,003. | large inocula of stenotrophomonas maltophilia vun 10,003 were used to investigate bacterial degradation of benzo[a]pyrene and dibenz[a,h]anthracene. although strain vun 10,003 was capable of degrading 10-15 mg (-1) of the five-ring compounds in the presence of pyrene after 63 days, further addition of pyrene after degradation of the five-ring polycyclic aromatic hydrocarbons (pahs) ceased did not stimulate significant decreases in the concentration of benzo[a]pyrene or dibenz[a,h]anthracene. how ... | 2002 | 12074057 |
| keratin degradation: a cooperative action of two enzymes from stenotrophomonas sp. | a novel keratin-degrading bacterium stenotrophomonas sp. strain d-1, isolated from deer fur, produced two types of extracellular proteins: proteolytic and disulfide bond-reducing. the results on the biochemical properties suggest that this protease belongs to the serine protease, and the disulfide bond-reducing protein could be the disulfide reductase type. none of these enzymes showed keratinolytic activity independently. however, after mixing of the two enzymes, the keratinolytic activity was ... | 2002 | 12074595 |
| comparative antimicrobial spectrum and activity of the desfluoroquinolone bms284756 (t-3811) tested against non-fermentative gram-negative bacilli. | to compare the in vitro activity of bms284756, a desfluoroquinolone, with four fluoroquinolones against 129 pseudomonas aeruginosa strains, 97 stenotrophomonas/burkholderia group strains and 43 acinetobacter spp. strains by three in vitro test methods. | 2002 | 12084101 |
| [interpretative reading of the non-fermenting gram-negative bacilli antibiogram]. | among non-fermenting gram-negative rods, the most clinically important species are pseudomonas aeruginosa, acinetobacter baumannii, and stenotrophomonas maltophilia, which are frequently multiresistant. p. aeruginosa resistance to beta-lactams depends on the production of chromosomal and plasmid-mediated beta-lactamases, altered permeability (loss of oprd porin is related to carbapenem-resistance) and active efflux pumps, particularly mexab-oprm. in aminoglycoside-resistant strains the main mech ... | 2002 | 12084355 |
| highly antibacterial active aminoacyl penicillin conjugates with acylated bis-catecholate siderophores based on secondary diamino acids and related compounds. | new acylated bis-catecholates and 1,3-benzoxazine-2,4-dione derivatives based on secondary diamino acids (n-(aminoalkyl)glycines, n-aminopropyl-alanine, and n-aminopropyl-4-aminovaleric acid), on n-(aminoalkyl)aminomethyl benzoic acids, on n-(aminoalkyl)aminomethyl phenoxyacetic acids, or on 3,5-diaminobenzoic acid were synthesized as artificial siderophores. the corresponding diamino acids were obtained from the diamines and oxocarboxylic acids by catalytic hydrogenation. the acylated bis-catec ... | 2002 | 12086488 |