Publications

non-falciparum malaria infections in pregnant women in west africa.	non-plasmodium falciparum malaria infections are found in many parts of sub-saharan africa but little is known about their importance in pregnancy.	2016	26823277
widespread distribution of plasmodium vivax malaria in mauritania on the interface of the maghreb and west africa.	plasmodium vivax is very rarely seen in west africa, although specific detection methods are not widely applied in the region, and it is now considered to be absent from north africa. however, this parasite species has recently been reported to account for most malaria cases in nouakchott, the capital of mauritania, which is a large country at the interface of sub-saharan west africa and the maghreb region in northwest africa.	2016	26861780
malaria in children of tshimbulu (western kasai, democratic republic of the congo): epidemiological data and accuracy of diagnostic assays applied in a limited resource setting.	the literature data on malaria in western kasai, drc, are limited and inadequate. a recent molecular survey there has detected plasmodium ovale and plasmodium malariae as mixed infections with plasmodium falciparum. in tshimbulu, western kasai, during a humanitarian initiative designed to provide children with free preventive screening and to reduce the local high malaria death rate, accurate species identification was performed, in order to collect unambiguous epidemiological data and to evalua ...	2016	26864461
uk malaria treatment guidelines 2016.	1.malaria is the tropical disease most commonly imported into the uk, with 1300-1800 cases reported each year, and 2-11 deaths. 2. approximately three quarters of reported malaria cases in the uk are caused by plasmodium falciparum, which is capable of invading a high proportion of red blood cells and rapidly leading to severe or life-threatening multi-organ disease. 3. most non-falciparum malaria cases are caused by plasmodium vivax; a few cases are caused by the other species of plasmodium: pl ...	2016	26880088
assessment of point-of-care diagnostics for g6pd deficiency in malaria endemic rural eastern indonesia.	patients infected by plasmodium vivax or plasmodium ovale suffer repeated clinical attacks without primaquine therapy against latent stages in liver. primaquine causes seriously threatening acute hemolytic anemia in patients having inherited glucose-6-phosphate dehydrogenase (g6pd) deficiency. access to safe primaquine therapy hinges upon the ability to confirm g6pd normal status. carestart g6pd, a qualitative g6pd rapid diagnostic test (g6pd rdt) intended for use at point-of-care in impoverishe ...	2016	26894297
human malaria diagnosis using a single-step direct-pcr based on the plasmodium cytochrome oxidase iii gene.	nested pcrs based on the plasmodium 18s-rrna gene have been extensively used for human malaria diagnosis. however, they are not practical when large quantities of samples need to be processed, further there have been challenges in the performance and when interpreting results, especially when submicroscopic infections are analysed. here the use of "direct pcr" was investigated with the aim of improving diagnosis in the malaria elimination era.	2016	26928594
the risk of imported malaria in security forces personnel returning from overseas missions in the context of prevention of re-introduction of malaria to sri lanka.	sri lanka is a malaria-free country. however it remains surrounded by countries with endemic malaria transmission. since the last indigenous case of malaria was reported in october 2012, only imported malaria cases have been diagnosed with 36 cases detected in 2015, which includes 17 cases each of plasmodium vivax and plasmodium falciparum and two cases of plasmodium ovale.	2016	26955813
mouse-based research on quiescent primate malaria parasites.	mice engrafted with primate tissue make two important plasmodial dormancy-related questions researchable. the first is concerned with whether latent merozoites in the lymphatic system can give rise to relapse-like, recurrent malaria in primates. the second is that genetic evidence of hypnozoite activation as the source of relapsing primate malaria can be looked for.	2016	26961183
[epidemiologic characteristics of plasmodium ovale in china in 2011-2014].	to investigate the epidemiological characteristics of plasmodium ovale in 2011-2014 for malaria control policy making during the phase of malaria elimination in china.	2016	27029361
malaria in three epidemiological strata in mauritania.	malaria epidemiology in mauritania has been characterized on the basis of epidemiological strata, defined by climatic and geographic features, which divide the country into three zones: sahelian zone, sahelo-saharan transition zone, and saharan zone. the association between geographic stratification and malaria transmission was assessed through a series of parasitological and entomological surveys.	2016	27068219
clinical implications of a gradual dormancy concept in malaria.	malaria recurrences after an initially successful therapy and malarial fever occurring a long time after infection are well-known problems in malariology. currently, two distinct types of malaria recurrences are defined: recrudescence and relapse. a recrudescence is thought to originate from circulating plasmodium blood stages which do not cause fever before a certain level of a microscopically detectable parasitemia is reached. contrary, a relapse is thought to originate from quiescent intracel ...	2016	27079460
investigation on possible transmission of monkeys' plasmodium to human in a populations living in the equatorial rainforest of the democratic republic of congo.	plasmodiums are protozoa that may infect various hosts. only five species are now recognized as naturally parasitizing humans: plasmodium falciparum, plasmodium vivax, plasmodium malariae, plasmodium ovale and plasmodium knowlesi. this fifth species, p. knowlesi, previously identified as naturally parasitizing the monkey macaca fascicularis, has been microscopically confused for a long time with p. malariae or p. falciparum and it was not possible to correctly differentiate them until the advent ...	2016	27141437
seroepidemiology of plasmodium species infections in zimbabwean population.	individuals living in malaria-endemic regions may be exposed to more than one plasmodium species; there is paucity of data on the distribution of the different species of plasmodium in affected populations, in part due to the diagnostic method of microscopy, which cannot easily differentiate between the species. sero-epidemiological data can overcome some of the shortcomings of microscopy.	2016	27165412
common asymptomatic and submicroscopic malaria infections in western thailand revealed in longitudinal molecular and serological studies: a challenge to malaria elimination.	despite largely successful control efforts, malaria remains a significant public health problem in thailand. based on microscopy, the northwestern province of tak, once thailand's highest burden area, is now considered a low-transmission region. however, microscopy is insensitive to detect low-level parasitaemia, causing gross underestimation of parasite prevalence in areas where most infections are subpatent. the objective of this study was to assess the current epidemiology of malaria prevalen ...	2016	27333893
climate, environment and transmission of malaria.	malaria, the most common parasitic disease in the world, is transmitted to the human host by mosquitoes of the genus anopheles. the transmission of malaria requires the interaction between the host, the vector and the parasite.the four species of parasites responsible for human malaria are plasmodium falciparum, plasmodium ovale, plasmodium malariae and plasmodium vivax. occasionally humans can be infected by several simian species, like plasmodium knowlesi, recognised as a major cause of human ...	2016	27367318
genome-scale comparison of expanded gene families in plasmodium ovale wallikeri and plasmodium ovale curtisi with plasmodium malariae and with other plasmodium species.	malaria in humans is caused by six species of plasmodium parasites, of which the nuclear genome sequences for the two plasmodium ovale spp., p. ovale curtisi and p. ovale wallikeri, and plasmodium malariae have not yet been analyzed. here we present an analysis of the nuclear genome sequences of these three parasites, and describe gene family expansions therein. plasmodium ovale curtisi and p. ovale wallikeri are genetically distinct but morphologically indistinguishable and have sympatric range ...	2016	27392654
low prevalence of plasmodium malariae and plasmodium ovale mono-infections among children in the democratic republic of the congo: a population-based, cross-sectional study.	in an effort to improve surveillance for epidemiological and clinical outcomes, rapid diagnostic tests (rdts) have become increasingly widespread as cost-effective and field-ready methods of malaria diagnosis. however, there are concerns that using rdts specific to plasmodium falciparum may lead to missed detection of other malaria species such as plasmodium malariae and plasmodium ovale.	2016	27392905
transfusion-transmitted severe plasmodium knowlesi malaria in a splenectomized patient with beta-thalassaemia major in sabah, malaysia: a case report.	transfusion-transmitted malaria (ttm) is a well-recognized risk of receiving blood transfusions, and has occurred with plasmodium falciparum, plasmodium vivax, plasmodium ovale, and plasmodium malariae. the simian parasite plasmodium knowlesi is also known to be transmissible through inoculation of infected blood, and this species is now the most common cause of malaria in malaysia with a high rate of severity and fatal cases reported. no confirmed case of accidental transfusion-transmitted p. k ...	2016	27405869
persistent parasitism: the adaptive biology of malariae and ovale malaria.	plasmodium malariae causes malaria in humans throughout the tropics and subtropics. plasmodium ovale curtisi and plasmodium ovale wallikeri are sympatric sibling species common in sub-saharan africa and also found in oceania and asia. although rarely identified as the cause of malaria cases in endemic countries, pcr detection has confirmed all three parasite species to be more prevalent, and persistent, than previously thought. chronic, low-density, multispecies asymptomatic infection is a succe ...	2016	27480365
the host specificity of ape malaria parasites can be broken in confined environments.	recent studies have revealed a large diversity of plasmodium spp. among african great apes. some of these species are related to plasmodium falciparum, the most virulent agent of human malaria (subgenus laverania), and others to plasmodium ovale, plasmodium malariae and plasmodium vivax (subgenus plasmodium), three other human malaria agents. laverania parasites exhibit strict host specificity in their natural environment. plasmodium reichenowi, plasmodium billcollinsi, plasmodium billbrayi and ...	2016	27486075
non-falciparum malaria in dakar: a confirmed case of plasmodium ovale wallikeri infection.	plasmodium ovale is rarely described in senegal. a case of clinical malaria due to p. ovale wallikeri in west central of senegal is reported.	2016	27557982
the increasing importance of plasmodium ovale and plasmodium malariae in a malaria elimination setting: an observational study of imported cases in jiangsu province, china, 2011-2014.	following initiation of china's national malaria elimination action plan in 2010, indigenous malaria infections in jiangsu province decreased significantly. meanwhile imported plasmodium infections have increased substantially, particularly plasmodium ovale and plasmodium malariae. given the risk for malaria resurgence, there is an urgent need to understand the increase in imported p. ovale and p. malariae infections as china works to achieve national malaria elimination.	2016	27604629
characteristics of imported malaria and species of plasmodium involved in shandong province, china (2012-2014).	malaria remains a serious public health problem in shandong province, china; therefore, it is important to explore the characteristics of the current malaria prevalence situation in the province. in this study, data of malaria cases reported in shandong during 2012-2014 were analyzed, and plasmodium species were confirmed by smear microscopy and nested-pcr. a total of 374 malaria cases were reported, 80.8% of which were reported from 6 prefectures. of all cases, p. falciparum was dominant (81.3% ...	2016	27658591
screening for glucose-6-phosphate dehydrogenase deficiency using three detection methods: a cross-sectional survey in southwestern uganda.	despite the potential benefit of primaquine in reducing plasmodium falciparum transmission and radical cure of plasmodium vivax and plasmodium ovale infections, concerns over risk of hemolytic toxicity in individuals with glucose-6-phosphate dehydrogenase deficiency (g6pdd) have hampered its deployment. a cross-sectional survey was conducted in 2014 to assess the g6pdd prevalence among 631 children between 6 and 59 months of age in southwestern uganda, an area where primaquine may be a promising ...	2016	27672207
molecular evidence of high rates of asymptomatic p. vivax infection and very low p. falciparum malaria in botswana.	botswana is one of eight sadc countries targeting malaria elimination by 2018. through spirited upscaling of control activities and passive surveillance, significant reductions in case incidence of plasmodium falciparum (0.96 - 0.01) was achieved between 2008 and 2012. as part of the elimination campaign, active detection of asymptomatic plasmodium species by a highly sensitive method was deemed necessary. this study was carried out to determine asymptomatic plasmodium species carriage by nested ...	2016	27682611
dissecting malaria biology and epidemiology using population genetics and genomics.	molecular approaches have an increasingly recognized utility in surveillance of malaria parasite populations, not only in defining prevalence and incidence with higher sensitivity than traditional methods, but also in monitoring local and regional parasite transmission patterns. in this review, we provide an overview of population genetic and genomic studies of human-infecting plasmodium species, highlighting recent advances in the field. in accordance with the renewed impetus for malaria eradic ...	2017	27825828
parasitological correlates of plasmodium ovale curtisi and plasmodium ovale wallikeri infection.	malaria, due to plasmodium ovale, can be challenging to diagnose due to clinically mild disease and low parasite burden. two genetically distinct sub-species of p. ovale exist: plasmodium ovale curtisi (classic) and plasmodium ovale wallikeri (variant). it is presently unknown if the sub-species causing infection affects performance of malaria diagnostic tests. the aim of this work was to understand how the genetically distinct sub-species, p. o. curtisi and p. o. wallikeri, affect malaria diagn ...	2016	27832785
low prevalence of laboratory-confirmed malaria in clinically diagnosed adult women from the wakiso district of uganda.	the malaria burden in sub-saharan africa (ssa) has fallen substantially. nevertheless, malaria remains a serious health concern, and uganda ranks third in ssa in total malaria burden. epidemiological studies of adult malaria in uganda are scarce and little is known about rates of malaria in non-pregnant adult women. this pilot study assessed malaria prevalence among adult women from wakiso district, historically a highly malaria endemic region.	2016	27842555
plasmodium malariae and plasmodium ovale infections in the china-myanmar border area.	the greater mekong subregion is aiming to achieve regional malaria elimination by 2030. though a shift in malaria parasite species predominance by plasmodium vivax has been recently documented, the transmission of the two minor plasmodium species, plasmodium malariae and plasmodium ovale spp., is poorly characterized in the region. this study aims to determine the prevalence of these minor species in the china-myanmar border area and their genetic diversity.	2016	27846879
evidence of non-plasmodium falciparum malaria infection in kédougou, sénégal.	expanded malaria control efforts in sénégal have resulted in increased use of rapid diagnostic tests (rdt) to identify the primary disease-causing plasmodium species, plasmodium falciparum. however, the type of rdt utilized in sénégal does not detect other malaria-causing species such as plasmodium ovale spp., plasmodium malariae, or plasmodium vivax. consequently, there is a lack of information about the frequency and types of malaria infections occurring in sénégal. this study set out to bette ...	2017	28049489
lamp kit for diagnosis of non-falciparum malaria in plasmodium ovale infected patients.	microscopy and rapid diagnosis tests have a limited sensitivity in diagnosis of malaria by plasmodium ovale. the lamp kit (loopamp®) can be used in the field without special equipment and could have an important role in malaria control programmes in endemic areas and for malaria diagnosis in returned travellers. the performance of the pan primer of the kit in detecting malaria by p. ovale was compared with the results of standard npcr in samples of patients returning from p. ovale endemic areas.	2017	28061871
two sympatric types of plasmodium ovale and discrimination by molecular methods.	plasmodium ovale is widely distributed in tropical countries, whereas it has not been reported in the americas. it is not a problem globally because it is rarely detected by microscopy owing to low parasite density, which is a feature of clinical ovale malaria. p.o. curtisi and p.o. wallikeri are widespread in both africa and asia, and were known to be sympatric in many african countries and in southeast asian countries. small subunit ribosomal rna (ssurrna) gene, cytochrome b (cytb) gene, and m ...	2016	28065415
dat-positive plasmodium ovale malaria presenting in a child with sickle cell anemia.		2017	28211104
a systematic review of the clinical presentation, treatment and relapse characteristics of human plasmodium ovale malaria.	despite increased efforts to control and ultimately eradicate human malaria, plasmodium ovale malaria is for the most part outside the focus of research or public health programmes. importantly, the understanding of p. ovale-nowadays regarded as the two distinct species p. ovale wallikeri and p. ovale curtisi-largely stems from case reports and case series lacking study designs providing high quality evidence. consecutively, there is a lack of systematic evaluation of the clinical presentation, ...	2017	28284211
molecular characterization and phylogenetic analysis of plasmodium vivax, plasmodium falciparum, plasmodium ovale, plasmodium malariae and plasmodium cynomolgi.	18s ribosomal rna gene sequences of different species of plasmodium were aligned and analyzed to determine the molecular diversity among different species of plasmodium. at content of p. cynomolgi, p. ovale, p. falciparum, p. vivax and p. malariae ranged from 62.30 to 63.15, 63.90 to 65.29, 66.67 to 68.40, 61.66 to 63.25 and 64.09 to 76.36 in case respectively. gc content of p. cynomolgi, p. ovale, p. falciparum, p. vivaxand p. malariae ranged from 36.85 to 37.70, 34.71 to 36.43, 31.60 to 33.27, ...	2017	28316417
[role of primaquine in malaria control and elimination in french-speaking africa].	primaquine, an 8-aminoquinoline, is a relatively unknown and underutilized drug in french-speaking african countries. it acts against the liver stage parasites of all human malaria species, asexual blood stages of plasmodium vivax and, to a lesser degree, plasmodium falciparum; p. falciparum mature gametocytes, and p. vivax and plasmodium ovale hypnozoites. gastrointestinal disturbances are its most common side effects. the clinical utility of primaquine is limited due to its hematological side ...	2017	28417346
metal-dna interactions improve signal in high-resolution melting of dna for species differentiation of plasmodium parasite.	the success of high-resolution melting (hrm) analysis for distinguishing similar dnas with minor base mismatch differences is limited. here, metal-mediated structural change in dna has been exploited to amplify hrm signals leading to differentiation of target dnas in an orthologous gene corresponding to four plasmodium species. conserved 26-mer ssdnas from ldh gene of the four plasmodium species were employed as targets. a capture probe (cp) that is fully complementary to the plasmodium falcipar ...	2017	28421327
case report of plasmodium ovale curtisi malaria in sri lanka: relevance for the maintenance of elimination status.	following its recent certification as malaria-free, imported infections now pose the greatest threat for maintaining this status in sri lanka. imported infections may also introduce species that are uncommon or not previously endemic to these areas. we highlight in this case report the increasing importance of less common malaria species such as plasmodium ovale in elimination settings and discuss its relevance for the risk of malaria resurgence in the country.	2017	28438137
nested multiplex pcr for identification and detection of human plasmodium species including plasmodium knowlesi.	to develop a new technique for diagnosis of plasmodium knowlesi and at the same time to be able to discriminate among the diverse species of plasmodium causing human malaria.	2017	28442114
evaluation of the utility value of three diagnostic methods in the detection of malaria parasites in endemic area.	malaria is a debilitating disease with high morbidity and mortality in africa, commonly caused by different species of the genus plasmodium in humans. misdiagnosis is a major challenge in endemic areas because of other disease complications and technical expertise of the medical laboratory staff. microscopic method using giemsa-stained blood film has been the mainstay of diagnosis of malaria. however, since 1993 when rapid diagnostic test (rdt) kits were introduced, they have proved to be effect ...	2017	28477621
malaria pathogenesis.	in the mosquito-human life cycle, the six species of malaria parasites infecting humans (plasmodium falciparum, plasmodium vivax, plasmodium ovale wallickeri, plasmodium ovale curtisi, plasmodium malariae, and plasmodium knowlesi) undergo 10 or more morphological states, replicate from single to 10,000+ cells, and vary in total population from one to many more than 10(6) organisms. in the human host, only a small number of these morphological stages lead to clinical disease and the vast majority ...	2017	28533315
[spontaneous spleen rupture with p. ovale malaria].		2017	28545674
molecular and immunological analyses of confirmed plasmodium vivax relapse episodes.	relapse infections resulting from the activation hypnozoites produced by plasmodium vivax and plasmodium ovale represent an important obstacle to the successful control of these species. a single licensed drug, primaquine is available to eliminate these liver dormant forms. to date, investigations of vivax relapse infections have been few in number.	2017	28558712
imported malaria in pregnant women experienced in japan.	with ever-growing global exchanges, the number of travelers, including pregnant women, to the tropics is increasing, which poses a risk of contracting malaria. although there are several reports on imported malaria in pregnancy from western countries, those focusing on cases experienced in japan are very limited.	2017	28583709
molecular diagnosis of plasmodium ovale by photo-induced electron transfer fluorogenic primers: pet-pcr.	accurate diagnosis of malaria infections continues to be challenging and elusive, especially in the detection of submicroscopic infections. developing new malaria diagnostic tools that are sensitive enough to detect low-level infections, user friendly, cost effective and capable of performing large scale diagnosis, remains critical. we have designed novel self-quenching photo-induced electron transfer (pet) fluorogenic primers for the detection of p. ovale by real-time pcr. in our study, a total ...	2017	28640824
[biological disturbances affecting people with malaria: about thirty cases].		2017	28674567
a dual colour fluorescence in situ hybridization (fish) assay for identifying the zoonotic malaria parasite plasmodium knowlesi with a potential application for the specific diagnosis of knowlesi malaria in peripheral-level laboratories of southeast asia.	plasmodium knowlesi is primarily responsible for zoonotic malaria in several southeast asian countries. precise identification of the parasite in the blood of patients presently relies on an expensive and elaborate pcr procedure because microscopic examination of blood and other available field identification techniques lack adequate specificity. therefore, the use of a simple and inexpensive dual-colour fluorescence in situ hybridization (fish) assay, analogous to fish assays recently described ...	2017	28724402
non-falciparum malaria imported mainly from africa: a review from a portuguese hospital.	non-falciparum malaria (nfm) has been reported to be responsible for around 25% of imported malaria cases in europe but is often neglected due to its less severe clinical course when compared to plasmodium falciparum. differentiation between species is however crucial for a correct approach. the objective of this study is to report the cases of this often missed aetiology of malaria in a tertiary hospital in portugal.	2017	28743266
cryptic plasmodium ovale concurrent with mixed plasmodium falciparum and plasmodium malariae infection in two children from central african republic.	since several malaria parasite species are usually present in a particular area, co-infections with more than one species of plasmodium are more likely to occur in humans infected in these areas. in many mixed infections, parasite densities of the cryptic species may be low and often not recognized in clinical practice.	2017	28810865
malaria in the guangxi zhuang autonomous region in china: a twelve-year surveillance data study.	the incidence of an indigenous malaria, defined as malaria acquired by a local mosquito transmission, declined from 2004 to 2015 in the guangxi zhuang autonomous region. however, imported malaria, defined as malaria acquired from other endemic regions outside of china, has been increasing in the region, as in the rest of the country, particularly the disease caused by plasmodium falciparum. a retrospective study was conducted to explore malaria-endemic characteristics in guangxi during the 2004- ...	2017	28820683