Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| studies on brucella melitensis rev. 1 vaccine in goats. | 1981 | 7342582 | |
| granulomas in brucella melitensis infection. | 1980 | 7352742 | |
| brucellosis treated with rifampicin. | 14 children, aged between 15 months and 14 years, with brucellosis were treated with oral rifampicin only (20 mg/kg per day) for 3 weeks. laboratory diagnosis depended on blood culture (positive for brucella melitensis in 11 of the cases), serum agglutination, complement-fixation test, and coomb's test. response was good in each child, with fever clearing between the 2nd and 8th day. 2 children relapsed, but one relapse was probably a reinfection from contaminated cheese. both children were give ... | 1980 | 7436494 |
| [brucella melitensis endocarditis]. | 1980 | 7450582 | |
| restriction site polymorphism of the genes encoding the major 25 kda and 36 kda outer-membrane proteins of brucella. | seventy-seven brucella reference and field strains from different geographic origins and hosts representing the six recognized species and their different biovars were analysed for diversity of their genes encoding the major 25 and 36 kda outer-membrane proteins (omps) by pcr-rflp. the 25 kda omp is encoded by a single gene (omp25) whereas two closely related genes (omp2a and omp2b) encode and potentially express the 36 kda omp. analysis of pcr products of the omp25 gene digested with nine restr ... | 1995 | 7496522 |
| in-vitro and in-vivo immunobiological properties of murine monoclonal anti-brucella antibodies. | features and protective activity of anti-brucella murine monoclonal antibodies (mabs) versus b. melitensis 16m were studied. all mabs tested were able to confer a good passive protection and showed correlative biological properties in immunological reactions. | 1994 | 7510505 |
| monoclonal antibodies to brucella rough lipopolysaccharide: characterization and evaluation of their protective effect against b. abortus. | we characterized 4 monoclonal antibodies (mab) specific for rough lipopolysaccharide (r-lps) of brucella. mab were selected by enzyme-linked immunosorbent assay (elisa) on whole b. abortus 45/20 rough cells and r-lps from b. melitensis b115 rough cells. specificity was confirmed by immunoblot analysis using r-lps and smooth lps (s-lps) preparations. anti-r-lps revealed the low molecular mass r-lps molecules below 20.1 kda in the r-lps and s-lps preparations as well as the typical a and m pattern ... | 1993 | 7514802 |
| brucella antigens--old dogmas, new concepts. | a brief review is given on concepts regarding brucella antigens, principally the lipopolysaccharide (lps) and o-polysaccharide but also, to a minor extent, proteins and lipids. although these views were reported years ago in the literature and became widely accepted at the time, many have since been disproven with results that have led to exciting new avenues of research and application. where gaps, contradictions and anecdotal observations suggest that the current understanding of brucella is l ... | 1993 | 7519357 |
| brucella melitensis cell envelope protein and lipopolysaccharide epitopes involved in humoral immune responses of naturally and experimentally infected sheep. | cell envelope fraction (cef) of brucella melitensis b115 was used to investigate antibody responses of b. melitensis naturally and strain h38 experimentally infected sheep by immunoblotting, indirect enzyme-linked immunosorbent assay (elisa) (i-elisa), and competitive elisa (c-elisa) with monoclonal antibodies (mabs). mabs used were directed to outer membrane proteins with molecular masses of 10, 16.5, 19, 25 to 27, 31 to 34, 36 to 38, and 89 kda; to the heat shock protein dnak, to o-polysacchar ... | 1994 | 7529242 |
| antibacterial activity of azithromycin against brucella melitensis. | in vitro antibacterial activity of the new macrolide azithromycin was tested against 116 strains of brucella melitensis, isolated from 115 patients in a major tertiary care referral center. eighty-seven percent of the strains were inhibited by 1.0 mg/l and all the 116 strains by 2.0 mg/l of azithromycin. comparison was made with tetracycline, gentamicin, trimethoprim-sulfamethoxazole, rifampicin and ciprofloxacin. all the isolates were susceptible to gentamicin, tetracycline, trimethoprim-sulfam ... | 1995 | 7555205 |
| surface exposure of outer membrane protein and lipopolysaccharide epitopes in brucella species studied by enzyme-linked immunosorbent assay and flow cytometry. | seven surface-exposed outer membrane proteins (omps) in brucella supp. have been previously described (a. cloeckaert, p. de wergifosse, g. dubray, and j. n. limet, infect. immun. 58:3980-3987, 1990). omps were shown to be more accessible to monoclonal antibodies (mabs) on rough (r) brucella melitensis and b. abortus strains than to mabs on their smooth (s) counterparts. in this work, we have extended this study to representatives of the main brucella species, using mabs specific for omps and s a ... | 1995 | 7558303 |
| comparative evaluation of microagglutination test and serum agglutination test as supplementary diagnostic methods for brucellosis. | the diagnosis of brucellosis in cattle and small ruminants requires the use of more than one serological test. the complement fixation test (cft), the rose bengal test (rbt), and the serum agglutination test (sat) are among the most useful tests for routine diagnosis. the microagglutination test (mat) was developed as a simpler and more efficient test than the sat. the relative efficacy of this test compared with that of the sat was evaluated by using brucella-free sheep and goats prior to and a ... | 1995 | 7559970 |
| thyroiditis due to brucella melitensis. | a case of thyroiditis due to brucella melitensis is reported. brucellosis anticipated by about two months the onset of the characteristic symptoms of acute thyroiditis. cultures of specimens obtained by fine needle aspiration biopsy and microbiological investigations allowed isolation and identification of the germ. this observation allowed the recognition that thyroid gland might harbored secondary localization of a prolonged brucellosis. a microbiological study (the protocol of which is propos ... | 1995 | 7560815 |
| growth phase-dependent variations in the outer membrane protein profile of brucella melitensis. | changes in brucella cell envelope protein profiles were investigated with batch cultures of b. melitensis strain 16m in a 2-litre fermenter. analysis of expression of outer membrane proteins (omp) (apparent molecular masses of 10, 16.5, 19, 25-27, 31-34, 36-38 and 89 kda) and heat-shock protein dnak (73 kda) was performed with monoclonal antibodies (mab) and immunoblotting techniques. synthesis of the 89-kda omp and the heat-shock protein dnak was invariant during b. melitensis growth. expressio ... | 1995 | 7569317 |
| study of the organization of the genomes of escherichia coli, brucella melitensis and agrobacterium tumefaciens by insertion of a unique restriction site. | tn5map, a tn5 derivative containing the 18 bp i-scei site, was delivered from a rp4-mobilizable, rk6-derived suicide vector to escherichia coli hb101, brucella melitensis and agrobacterium tumefaciens c58, which all lack natural i-scei sites in their genomes. digestion of the dna from tn5map-containing strains and analysis by pulsed-field gel electrophoresis (pfge) revealed that these derivatives contained a single transposon insertion. these digests also gave direct and independent proof for th ... | 1995 | 7582002 |
| evaluation of an enzyme-linked immunosorbent assay for the detection of sheep infected and vaccinated with brucella melitensis. | an indirect enzyme-linked immunosorbent assay (elisa), using the lipopolysaccharide of the cell wall as an antigen, was used to detect brucella melitensis antibodies in ovine serum. the test was carried out on 703 samples of field serums, which were also analyzed by the complement fixation (cf) test and the rose bengal (rb) test. the elisa results were more similar to those of the cf test (kappa = 0.89) than to the results of the rb test (kappa = 0.73). the elisa also had high sensitivity (94.7% ... | 1995 | 7619903 |
| characteristics of and risk factors for relapse of brucellosis in humans. | for 16 years we prospectively observed 530 adult patients with brucellosis to analyze the characteristics of and risk factors for relapse. clinical and laboratory findings from 86 relapsed patients were milder during the relapse episode when compared with those for the same patients during the initial disease. blood cultures were positive for brucella melitensis in 65% of cases during relapse and in approximately 80% of cases during the initial disease. risk factors that were identified as being ... | 1995 | 7620005 |
| comparison of the efficacy of brucella suis strain 2 and brucella melitensis rev. 1 live vaccines against a brucella melitensis experimental infection in pregnant ewes. | the comparative efficacy of brucella suis strain 2 (s2) and brucella melitensis strain rev. 1 (rev. 1) live vaccines in protecting sheep against b. melitensis infection was evaluated by clinical and bacteriological examination of ewes vaccinated conjunctivally with a dose of 1 x 10(9) c.f.u. when 4 months old and then challenged with 5 x 10(7) c.f.u. of the b. melitensis virulent strain 53h38 (h38) at the middle of the first or second pregnancy following vaccination. animals were considered to b ... | 1995 | 7625115 |
| susceptibilities of brucella melitensis isolates to clinafloxacin and four other new fluoroquinolones. | the susceptibilities of 120 clinical isolates of brucella melitensis and 3 reference strains of the same species to six fluoroquinolones (clinafloxacin, pd 117596, pd 131628, pd 138312, pd 140248, and ciprofloxacin) were examined by agar dilution mic methodology. clinafloxacin was the most active compound tested (mic at which 50% of strains tested were inhibited [mic50] and mic90 of 0.06 micrograms/ml). its level of activity was slightly higher than that of pd 117596 (mic50 and mic90 of 0.12 mic ... | 1995 | 7625815 |
| deletion of pure attenuates brucella melitensis 16m for growth in human monocyte-derived macrophages. | we constructed a defined purine-auxotrophic mutant of brucella melitensis 16m by chromosomal gene replacement. we electroporated b. melitensis 16m with suicide plasmids containing a kanamycin resistance cassette that replaced 226 bp at the carboxyl end of pure, the intergenic region, and 18 bases of the purk open reading frame. recombinant b. melitensis delta pure201 required exogenous purines for growth on minimal media. purine auxotrophy was complemented by electroporation of b. melitensis del ... | 1995 | 7642258 |
| protective effect of brucella outer membrane complex-bearing liposomes against experimental murine brucellosis. | liposomes of stable multilamellar type, which previously demonstrated great efficiency in antibiotic transport, were used in this study as transport vehicles of antigenic extracts of brucella melitensis (hs: complex of lipopolysaccharide/phospholipids/outer membrane proteins). the incorporation of hs into positively charged liposomes produced a protective effect against experimental murine brucellosis when they were administered 1 day before or 2 days after infection, as the number of colony-for ... | 1995 | 7649445 |
| in vitro efficacy of several antimicrobial combinations against brucella canis and brucella melitensis strains isolated from dogs. | in vitro efficacy of tetracyclines, aminoglycosides, fluoroquinolones, rifampin and macrolides and their combinations against four brucella canis and two b. melitensis strains isolated from dogs was evaluated by means of inhibition tests: minimal inhibitory concentration (mic) and fractional inhibitory concentrations (fic) and by bactericidal tests: minimal bactericidal concentrations (mbc) and kill-time experiments. in inhibition studies, tetracyclines showed the lowest mics of all compounds te ... | 1995 | 7653024 |
| cloning and sequence analysis of a newly identified brucella abortus gene and serological evaluation of the 17-kilodalton antigen that it encodes. | a thus far unknown gene encoding a brucella abortus protein has been isolated from a lambda gt11 expression library probed with sera from brucella-infected sheep. sequence analysis of the cloned gene revealed the presence of an open reading frame of 158 amino acids encoding a protein of 17.3 kda (calculated molecular mass). the recombinant b. abortus protein, expressed in escherichia coli, and the corresponding brucella melitensis protein migrated at the same apparent molecular masses as shown b ... | 1995 | 7664168 |
| cell response to a salt-extractable and sonicated brucella melitensis 16m antigen in human brucellosis. | we compared the immunological responses of leukocytes taken from healthy negative controls, laboratory workers immunized with the phenol-insoluble french vaccine against brucellosis, patients acutely ill with brucellosis, and patients chronically infected with brucella melitensis. a salt-extractable antigen (protein-rich but with traces of lipopolysaccharide) and a sonicated suspension from b. melitensis 16m were used as antigens for in vitro lymphocyte proliferation test. quantitation of t cell ... | 1995 | 7664186 |
| identification of sero-reactive brucella melitensis cytosoluble proteins which discriminate between antibodies elicited by infection and rev.1 vaccination in sheep. | distinction between brucella melitensis infected and vaccinated sheep is needed to fully achieve ovine brucellosis eradication in several countries. for this purpose, we probed immunoblots of cytosoluble protein extract (cpe) of the rough (r) b. melitensis strain b115 with sera of brucella-free, naturally infected, b. melitensis h38 experimentally infected and b. melitensis rev.1 vaccinated sheep to identify immunogenic brucella cytosoluble proteins which may lead to the development of more usef ... | 1995 | 7667905 |
| characterization of o-polysaccharide specific monoclonal antibodies derived from mice infected with the rough brucella melitensis strain b115. | twenty-two monoclonal antibodies (mabs) specific for smooth lipopolysaccharide (s-lps) were generated by fusion of spleen cells from mice infected with the rough brucella melitensis strain b115 with the nso myeloma. according to reactivity in enzyme-linked immunosorbent assay (elisa) with o-polysaccharide (o-ps) and absence of reactivity with rough lipopolysaccharide (r-lps), it was postulated that these mabs recognized epitopes present on the o-ps. most of the mabs reacted equally well in elisa ... | 1993 | 7690392 |
| cloning and sequencing of the bacterioferritin gene of brucella melitensis 16m strain. | the 40 n-terminal amino acids of the 20 kda antigen a2 from brucella melitensis were sequenced and showed important similarities with 4 bacterioferrins. a monoclonal antibody raised against this antigen cross-reacted with escherichia coli bacterioferritin. hybridization of two sets of degenerate primers with b. melitensis hindiii-digested genomic dna identified a 3.8 kb fragment. this fragment was shown to contain a bacterioferritin gene (bfr) encoding a 161-amino acid protein. the sequence of t ... | 1995 | 7698330 |
| pseudogout masking brucellar arthritis. | 1995 | 7728410 | |
| bacterial infections: osteoarticular brucellosis. | osteoarticular brucellosis has been documented extensively from the middle east and spain in the last 5 years, but it has only been reported infrequently from the uk and usa. brucella melitensis from goat and sheep is the most frequently isolated organism. peripheral articular pain, particularly of the large joints, is the commonest osteoarticular manifestation, while effusions that seldom yield organisms on culture, also occur frequently. sacroiliitis which most frequently is unilateral, often ... | 1995 | 7728879 |
| brucella melitensis: an unexpected isolate from cerebrospinal fluid. | a specimen of cerebrospinal fluid was initially handled with 'category 3' precautions because the patient came from somalia, where tuberculosis and hiv infection are endemic. an isolate from the specimen, initially thought to be a neisseria species, was subsequently handled on the open bench. it was later identified as brucella melitensis. laboratory procedures should allow for the possibility of brucella in such specimens until a positive identification has been made. | 1995 | 7735352 |
| specific bovine brucellosis diagnosis based on in vitro antigen-specific gamma interferon production. | in order to improve the specificity of the diagnosis of bovine brucellosis, we developed a test which can be regarded as an in vitro correlate of the delayed-type hypersensitivity test (dth). a mixture of cytoplasmic proteins from brucella melitensis b115 was used as a specific antigenic stimulus in bovine whole blood culture. supernatants harvested at 18 to 24 h after the in vitro antigenic stimulus were assayed for their gamma interferon (ifn-gamma) content by using a commercial sandwich enzym ... | 1995 | 7751381 |
| in vivo and in vitro stability of the broad-host-range cloning vector pbbr1mcs in six brucella species. | previous studies have shown that the broad-host-range plasmid pbbr1mcs can be used for genetic complementation in brucella abortus. to extend these observations, the in vivo and in vitro stability of pbbr1mcs was evaluated in the six currently recognized species of the genus brucella. pbbr1mcs was readily introduced into all of the strains tested by electroporation and was stably maintained in broth cultures without antibiotic selection during five serial passages over a 10-day period. furthermo ... | 1995 | 7753908 |
| [brucellar bursitis]. | 1995 | 7754159 | |
| enhanced cellular response in mice treated with a brucella antigen-liposome mixture. | the capacity of liposomes constituted by dycetyl-phosphate (0.009 mm), cholesterol (0.017 mm), lecithin (0.003 mm), and myristic (0.1 mm), stearic (0.1 mm), or oleic acid (0.1 mm) to modify the lymphocyte response to brucella melitensis antigens in mice was studied. mice treated with antigens mixed with liposomes containing myristic, stearic or oleic acid had higher antibody titres than mice given antigen suspended in a saline solution. liposomes alone, without brucella antigens, resulted in inc ... | 1995 | 7773240 |
| serological responses to brucellosis in hiv-seropositive patients. | serial sera from 2 patients infected with the human immunodeficiency virus (hiv) type 1 attending a clinic in nairobi, kenya, and with blood cultures yielding brucella melitensis, were tested by enzyme-linked immunosorbent assay for their serological response (brucella-specific immunoglobulin (ig) m and igg) to brucella infection. antibody responses were comparable to those of immunocompetent individuals, one patient showing serology typical of acute brucellosis, the other of chronic brucellosis ... | 1995 | 7778159 |
| osteoarticular brucellosis resembling microcrystalline arthropathy. | 1994 | 7799378 | |
| national cross-sectional study of mastitis in dairy cattle in jordan. | between july 1991 and august 1992, 63 jordanian dairy farms selected by stratified random sample were visited to identify the major causes and prevalence of intramammary infections in dairy cows. of 773 cows examined 60% of all sampled quarters had > 283,000 cells/ml. the mean value of somatic cell count (scc) was positively associated with age in lactations and negatively with herd size. cows milked by bucket milking machines or in fully automatic parlours had a lower mean scc than those milked ... | 1994 | 7809990 |
| secondary amenorrhoea due to persistent corpus luteum in brucellosis. | 1995 | 7823703 | |
| microangiopathic hemolytic anemia and severe thrombocytopenia in brucella infection. | a case of brucella septicemia presenting at the onset as a severe microangiopathic hemolytic anemia with coexisting dramatic hemorrhagic syndrome (severe epistaxis, gross hematuria, and skin purpura) is reported. a hemogram showed severe thrombocytopenia, anemia, and leukopenia. bone marrow morphology showed the typical features associated with brucella infection: numerous histiocytes with signs of activation, multiple granulomata, giant cells, and hemophagocytosis. after appropriate antimicrobi ... | 1995 | 7827209 |
| fusogenic activity of amino-terminal region of hiv type 1 nef protein. | we have studied two isoforms of nef, nef-27 and nef-25, which were produced in e. coli. nef-25 lacked the first 18 n-terminal residues of nef-27 and both were nonmyristylated. nef-27 fuses small unilamellar dipalmitoyl phosphatidylcholine vesicles (suvs), as indicated by enhanced light scattering of suvs and lipid mixing using concentration-dependent fluorescence dequenching. nef-27 also causes the appearance of a shifted isotropic peak in the 31p nmr spectra of these vesicles, suggesting that p ... | 1994 | 7848681 |
| differentiation of brucella abortus bv. 1, 2, and 4, brucella melitensis, brucella ovis, and brucella suis bv. 1 by pcr. | several pcr assays which identify the genus brucella but do not discriminate among species have been reported. we describe a pcr assay that comprises five oligonucleotide primers which can identify selected biovars of four species of brucella. individual biovars within a species are not differentiated. the assay can identify three biovars (1, 2, and 4) of b. abortus, all three biovars of b. melitensis, biovar 1 of b. suis, and all b. ovis biovars. these biovars include all of the brucella specie ... | 1994 | 7852552 |
| [detection of brucella with an automatic hemoculture system: bact/alert]. | the ability of in vitro and in vivo detection of brucella spp. with the bact/alert system was studied. | 1994 | 7865557 |
| immunotherapy in chronic brucellosis. effect of levamisole and interferon; mechanisms of action and clinical value. | thirty two anergic patients with chronic brucellosis treated with a) interferon-alpha 2b(group 1), b) levamisole (group 2) and c) conventional therapy(group 3) were studied. the effect of treatment on t lymphocyte blast formation in the presence of pha, specific cell mediated immunity against brucella antigens, titers of brucella antibodies and clinical symptoms were evaluated .t lymphocyte blast formation was shown to range in normal levels in all patients before treatment compared to 10 normal ... | 1994 | 7876467 |
| immunogenic properties of brucella melitensis cell-wall fractions in balb/c mice. | the immunogenicity of several brucella melitensis cell-wall (cw) fractions was tested in balb/c mice. these cw fractions were smooth lipopolysaccharide (s-lps) fraction from smooth (s) b. melitensis strain 16m, sodium dodecyl sulphate-insoluble (sds-i) cw fraction from b. melitensis strain 16m (s) undigested or digested with pepsin, and sds-i cw fraction from rough (r) b. melitensis strain h38. the b. melitensis sds-i cw fraction contained two major outer-membrane proteins (omps) of 25-27 kda an ... | 1995 | 7884802 |
| a case of brain abscess due to brucella melitensis. | 1994 | 7893897 | |
| brucella haemorrhagic pleural effusion. | brucella melitensis (bm) is a rare respiratory pathogen. the lungs are usually affected in the subacute and chronic course of the disease, when pleurisy, empyema, hilar adenopathy, pneumonia and lung abscess have been described. we present a patient with bm haemorrhagic pleural effusion, with low ph, low glucose and positive pleural fluid cultures. brucellosis should be considered in the differential diagnosis of patients with long-standing pleural effusions of unknown aetiology. | 1994 | 7925918 |
| vaccination with brucella abortus rough mutant rb51 protects balb/c mice against virulent strains of brucella abortus, brucella melitensis, and brucella ovis. | vaccination of balb/c mice with live brucella abortus rb51, a stable rough mutant, produced protection against challenge with virulent strains of brucella abortus, brucella melitensis, and brucella ovis. passive-transfer experiments indicated that vaccinated mice were protected against b. abortus 2308 through cell-mediated immunity, against b. ovis pa through humoral immunity, and against b. melitensis 16m through both forms of immunity. live bacteria were required for the induction of protectiv ... | 1994 | 7927779 |
| [the endotoxins of gram-negative bacteria: their structure and biological role]. | main attention in the paper is paid to the study of lipid a, a component possessing endotoxic activity. lipids a containing glucosamine disaccharide (representatives of enterobacteriaceae family), and variants of lipid a differing from the toxic one either in the structure of carbohydrate core or in the spectrum of fatty acids are considered. they are either phototrophic, nodulating (bradyrhyzobium species) or soil species (nitrobacter and thiobacillus) bacteria. lipid a from lipopolysaccharides ... | 1994 | 7952230 |
| brucella ribosomal protein l7/l12 is a major component in the antigenicity of brucellin inra for delayed-type hypersensitivity in brucella-sensitized guinea pigs. | a delayed-type hypersensitivity (dth) reaction in the course of brucellosis in humans and animals can be revealed by the brucellin inra (brucellergen) skin test. brucellergen is composed of more than 20 proteins of different molecular weights. a 12-kda protein eliciting dth in brucella melitensis rev1-sensitized guinea pigs was found to be a significant component for the allergenic properties of brucellergen. sequencing of the gene encoding this protein identified it as the l7/l12 ribosomal prot ... | 1994 | 7960115 |
| changing trends in the epidemiology of human brucellosis in california from 1973 to 1992: a shift toward foodborne transmission. | from 1973 through 1992, 426 cases of human brucellosis were reported in california, of which 98% were laboratory confirmed. brucella melitensis was identified in 185 cases (78.7% of the bacteriologically typed cases). hispanics accounted for 81% of the cases from 1983 to 1992 compared with 65% during the previous decade (p < .01). the population-adjusted average annual incidence was higher in hispanics, especially in children and teenagers, compared with non-hispanic whites and african americans ... | 1994 | 7963716 |
| [aortic valvular endocarditis caused by brucella melitensis with initial acute myocardial infarction manifestation]. | this is the case of a 31 year old man with brucella aortic valvular endocarditis which first manifested itself clinically as an acute myocardial infarction (ami). he subsequently developed an acute aortic regurgitation, which together with the ventricular disfunction secondary to the myocardial necrosis caused severe heart failure requiring aortic valve replacement and aortic coronary graft in a first operation. the patient's subsequent evolution was negative with congestive heart failure refrac ... | 1994 | 7973020 |
| evaluation of allergic and serological tests for diagnosing brucella melitensis infection in sheep. | a total of 291 unvaccinated sheep from brucella melitenesis-infected flocks were examined for delayed-type hypersensitivity (dth) responses with brucellergene commercial allergen and with cold saline extract and cytosol from rough b. melitensis 115, and their sera were tested in the rose bengal test (rbt), complement fixation test (cft), and enzyme-linked immunosorbent assay (elisa) with lipopolysaccharide. dth reactions were maximal after 72 h, with no intensity differences among allergens, ino ... | 1994 | 7989528 |
| detection of brucella melitensis by bactec nr660 blood culture system. | data on the performance of modern blood culture systems for the detection of brucella spp. are insufficient. to evaluate the performance of the bactec nr660 blood culture system for the detection of brucella melitensis within the routine 1-week blood culture protocol, a prospective 24-month study was conducted in an area endemic for b. melitensis in southern israel. blood samples obtained from patients with suspected brucellosis were monitored and blindly subcultured once per week for 4 weeks, a ... | 1994 | 7989539 |
| outbreak of brucella melitensis among microbiology laboratory workers. | we report on an outbreak of laboratory-acquired brucellosis involving four technicians working at a microbiology laboratory. all cases occurred in a period of 4 months. blood cultures and the rose bengal test were positive for brucella spp. in all cases. microagglutination was positive for brucella spp. at titers of between 1/40 and 1/160. all patients were cured after treatment. | 1994 | 7989566 |
| [an evaluation of the effectiveness of laboratory diagnostic methods for brucellosis]. | the diagnostic value of bacteriological and serological methods for the laboratory diagnosis of brucellosis was studied. in the analysis of milk and cheese specimens brucella cultures were isolated and differentiated as b.melitensis, biovar i, and b.abortus, biovar 4. in 25.6% of cases b.melitensis culture, biovar 1, was isolated from the blood of persons suspected for brucellosis. the isolation of b.melitensis culture from milk showed that this infective agent migrated from small animals to cat ... | 1994 | 7992538 |
| epidural abscess due to acute brucella melitensis infection. | 1994 | 8002693 | |
| efficacy of different rose bengal and complement fixation antigens for the diagnosis of brucella melitensis infection in sheep and goats. | fifteen different rose bengal antigens showed large differences with respect to ph, cell concentration and agglutination with the international standard anti-brucella abortus serum, demonstrating the lack of international standardisation. their sensitivity and specificity, compared with that of the complement fixation test, were evaluated for the diagnosis of b melitensis infection in culture-positive sheep, brucella-free ewes, and sheep and goats belonging to field flocks under different epidem ... | 1994 | 8036772 |
| efficacy of combinations of doxycycline and rifampicin in the therapy of experimental mouse brucellosis. | mice infected with brucella melitensis were treated with doxycycline and rifampicin in daily dosages of: 1.5, 3 and 6 mg/kg separately and in combination, for a period of 14 days. doxycycline alone in all dosages did not result in a cure rate superior to controls. a significant reduction in the viable count of b. melitensis recovered from the spleens of mice (which was the criterion for cure) was achieved with 6 and 3 mg/kg/day of doxycycline. rifampicin at a dose of 6 mg/kg/day gave significant ... | 1994 | 8040118 |
| evaluation of serological tests for diagnosis of brucella melitensis infection of goats. | five serological assays were evaluated for the diagnosis of brucellosis in goats: the rose bengal test (rbt), complement fixation test (cft), radial immunodiffusion (rid) with brucella and yersinia enterocolitica o:9 polysaccharides, counterimmunoelectrophoresis (ciep) with cytosol, and enzyme-linked immunosorbent assay (elisa) with polyclonal and protein g conjugates and smooth lipopolysaccharide (s-lps), native hapten polysaccharide (nh), or cytosol antigens. for optimal sensitivity, rbt had t ... | 1994 | 8051240 |
| fatal brucella melitensis endocarditis in a hemodialysis patient. | 1994 | 8072615 | |
| identification and nucleotide sequence of brucella melitensis l7/l12 ribosomal protein. | dna sequencing of the gene encoding a brucella melitensis 12-kda protein revealed that this protein was the ribosomal protein l7/l12. the b. melitensis l7/l12 dna sequence was identical to that of the corresponding b. abortus gene, showing the near identity of these two organisms. when comparing the sequence of this protein to that of other organisms some domains were highly conserved, especially the c-terminus, which contrasted with the lack of conservation of the sequences at the n-terminus. t ... | 1994 | 8076798 |
| activity of bay y 3118, a novel 4-quinolone, against brucella melitensis. | we have tested the in-vitro activities of bay y 3118, a new chlorofluoroquinolone, ciprofloxacin, sparfloxacin, streptomycin, tetracycline and rifampin against 59 strains of brucella melitensis. bay y 3118 (mic90 0.12 mg/l) was twice as active as sparfloxacin and tetracycline (mic90 0.25 mg/l). the activity of ciprofloxacin, rifampin and streptomycin (mic90 0.5, 2, and 8 mg/l, respectively) was, respectively, four-, sixteen-, and more than sixty-fold lower than that of bay y 3118. | 1994 | 8077983 |
| hematologic manifestations of brucellosis in children. | 1994 | 8078747 | |
| [the effect of immunomodulators on the development of the infectious and vaccinal processes as well as on the efficacy of therapeutic preparations in brucellosis]. | 1993 | 8079591 | |
| therapeutic effects of roxithromycin and azithromycin in experimental murine brucellosis. | mice infected with brucella melitensis were treated with azithromycin or roxithromycin at a dose of 50 mg/kg/day i.p. alone and in combination with streptomycin 75 mg/kg/day for 14 days. streptomycin at this dose was previously documented to be ineffective against murine brucellosis. azithromycin- and azithromycin/streptomycin-treated animals demonstrated a significantly better cure rate than controls. therapy failure was observed in all mice treated with roxithromycin 50 mg/kg/day i.p. alone or ... | 1994 | 8082413 |
| [the effect of the in-vitro interaction of antibacterial preparations on brucella melitensis]. | the character of drug interaction in 18 various combinations of sisomicin, amikacin, gentamicin, cefotaxime, doxycycline, trimethoprim and nitroxaline was studied by the chess board method with respect to 25 strains of brucella melitensis. the synergistic effect with respect to the majority of the cultures was observed with the use of the combinations of gentamicin, amikacin or sisomicin with doxycycline. the combinations of the aminoglycosides with cefotaxime and sisomicin or gentamicin with ri ... | 1993 | 8085903 |
| esophageal brucellosis: a new location of brucella infection. | the case of a patient with acute brucellosis presenting as lower dysphagia from a paracardial esophageal mass is described. brucella melitensis biotype 3 was isolated from cultured esophageal biopsy material. this is the first case of esophageal brucellosis described in the literature. | 1994 | 8086774 |
| brucella melitensis rev-1 vaccine as a cause of human brucellosis. | 1993 | 8103891 | |
| unusual suppurative complications of brucellosis in children. | three cases with suppurative complications of brucella melitensis infection are presented, demonstrating localized involvement of the brain, bones and lungs, respectively, and the lesions were well defined on radiographic examination. the diagnosis was made on the basis of significant brucella titres and positive blood culture. awareness of such complications and performance of the appropriate serological and bacteriological studies will establish the diagnosis and will also differentiate this d ... | 1993 | 8111184 |
| brucella melitensis growth on loewenstein-jensen egg medium from a case of brucella meningitis. | bacteriologic investigation of a cerebrospinal fluid (csf) specimen for mycobacteria on lowenstein-jensen egg medium revealed the presence of brucella organisms. this coincided with a significant antibody titer against brucella spp. this is the first documented report of the ability of b. melitensis organisms to grow on loewenstein-jensen egg medium. | 1993 | 8112041 |
| identification of an immunoreactive brucella abortus htra stress response protein homolog. | an 11-kb fragment of brucella abortus genomic dna cloned into the bamhi site of puc9 expressed a 60-kda protein in escherichia coli dh5-alpha. antibodies reactive with this 60-kda protein were detected by western blot (immunoblot) analysis in sera from mice, cattle, and goats experimentally infected with b. abortus, in sera from mice experimentally infected with brucella melitensis, and in serum from a dog experimentally infected with brucella canis. similar results were seen with sera obtained ... | 1994 | 8112833 |
| identification and sequence analysis of is6501, an insertion sequence in brucella spp.: relationship between genomic structure and the number of is6501 copies. | an insertion sequence (is) element of brucella ovis, named is6501, was isolated and its complete nucleotide sequence determined. is6501 is 836 bp in length and occurs 20-35 times in the b. ovis genome and 5-15 times in other brucella species. analysis of the junctions at the sites of insertion revealed a small target site duplication of four bases and inverted repeats of 17 bp with one mismatch. is6501 presents significant similarity (53.4%) with is427 identified in agrobacterium tumefaciens, su ... | 1993 | 8126444 |
| pleural fluid characteristics in pulmonary brucellosis. | although pulmonary symptoms accompany up to 16% of cases of infection with brucella melitensis, pleural effusion has rarely been reported. a 12 year old girl had brucellosis with pulmonary disease and a pleural effusion. the pleural fluid was clear and straw coloured with 2700 leucocytes/mm3 (93% lymphocytes), a protein level of 48 g/1, and a glucose concentration of 4.1 mmol/l. culture of the pleural fluid grew br melitensis. | 1994 | 8153949 |
| urban outbreak of a brucella melitensis infection in an argentine family: clinical and diagnostic aspects. | an outbreak of brucella melitensis in a family was studied. from the fourteen family members who ate unpasteurized goat cheese nine became ill. patients included four females and five males of 8 to 75 years old. in seven of the patients the diagnosis was confirmed by positive blood culture for b. melitensis biovar 1. all the patients were analyzed by standard tube agglutination (sta) and standard tube agglutination with 2-mercaptoethanol (sta-2me) tests at the time of diagnosis. in six of the pa ... | 1994 | 8156050 |
| brucellosis: an occupational hazard for medical laboratory personnel. report of five cases. | five cases of laboratory-acquired infection with brucella melitensis are reported. this pathogen is highly contagious when handled in the laboratory. clinicians should alert technologists when brucellosis is suspected so that specimens are handled under the most stringent safety measures. serological surveys and instructions of laboratory workers regarding the clinical diversity of the disease are helpful for early recognition of secondary cases. on the other hand, clinicians should consider bru ... | 1994 | 8181839 |
| [osteoarthritis caused by brucella melitensis: isolation of the microorganism from the synovial fluid]. | brucellosis is an unusual disease in childhood but the organ manifestations are even more unusual. the authors describe an osteoarthritis caused by brucella melitensis, localized at the tarsal scaphoid in a three years old calabrian child. | 1993 | 8197028 |
| antibody response to brucella melitensis outer membrane antigens in naturally infected and rev1 vaccinated sheep. | sera from brucella infected and b. melitensis rev1 vaccinated sheep were analysed by immunoblotting using the cell envelope fraction (cef) of b. melitensis b115. the cef of b. melitensis b115 was analysed using a bank of monoclonal antibodies. the fraction consisted mainly of s-lps like molecules, r-lps and outer membrane proteins (omps) of molecular masses of 10, 16.5, 19, 25-27, 31-34, 36-38, 73 and 89 kda. immunoblot analysis indicates that the antibody response in infected sheep was mainly d ... | 1994 | 8203126 |
| brucellosis presenting as prolonged fever and hemophagocytosis. | 1993 | 8213671 | |
| successful therapeutic regimens for treating brucella melitensis and brucella abortus infections in cows. | three therapeutic regimens were evaluated in 121 cows naturally infected with brucella melitensis or brucella abortus, using a combination of long-acting oxytetracycline (la-otc), streptomycin (st) and otc-intramammary infusion (imi). cessation of shedding of brucella in udder secretions and absence of brucella in selected tissues were considered criteria for successful treatment. regimen a (tested on 35 cows) consisted of la-otc 25 mg/kg administered intramuscularly (i.m.) every 3 days for 42 d ... | 1993 | 8219341 |
| anti-brucella activity of ro 23-9424, a dual-action antibacterial. | the in vitro activity of dual-acting antibacterial ro 23-9424 was determined by the agar dilution method against 126 clinical isolates of brucella melitensis. it was compared with fleroxacin, ciprofloxacin and five conventional drugs. mic50 and mic90 for ro 23-9424 were 1.0 and 4.0 mg/l, respectively, as compared with 0.25 and 0.5 mg/l for fleroxacin and ciprofloxacin. one strain which was resistant to other fluoroquinolones, with an mic of > 8.0 mg/l, did not show cross-resistance to ro 23-9424 ... | 1993 | 8222864 |
| brucella peritonitis. | we report the case of a patient with peritonitis caused by brucella melitensis who also had chronic liver disease. at first the patient was treated for bacterial peritonitis but when a lymphocytic ascites was aspirated antituberculosis chemotherapy was given. however, the serological tests for brucellosis were strongly positive and subsequently b. melitensis was isolated from ascitic fluid. | 1993 | 8228298 |
| prevalence of camel brucellosis in libya. | sera of 967 camels of both sexes were tested for antibodies to brucella using the rose bengal plate test, serum agglutination test and the complement fixation test. the prevalence of positive sera was 4.1 per cent. samples collected for cultural examination revealed 9 isolates. five isolates were from milk samples, 3 from aborted foetuses and one from a vaginal swab. all isolates were identified and biotyped as brucella melitensis biovar 1. | 1993 | 8236486 |
| evaluation of whole cell and subcellular vaccines against brucella ovis in rams. | five antigen preparations from brucella ovis strain reo 198 were incorporated with the pluronic polymer l-121 and muramyl dipeptide and tested as vaccines against b. ovis infection of rams. the antigenic preparations were: (1) a fraction enriched in outer membrane proteins and rough lipopolysaccharide (hot saline extract, hs); (2) the proteins from hs substantially free of lipopolysaccharide; (3) outer membrane blebs; (4) outer membrane-peptidoglycan complexes extracted with detergent; (5) kille ... | 1993 | 8236802 |
| quinolones in treatment of human brucellosis: comparative trial of ofloxacin-rifampin versus doxycycline-rifampin. | quinolones have been reported to be active against brucella species in vitro. in this prospective randomized study, the efficacy and safety of the combination of ofloxacin plus rifampin were compared with the efficacy and safety of doxycycline plus rifampin, both combinations administered for a 6-week period in treatment of brucellosis. sixty-one patients were enrolled in the study, and 49 had blood or bone marrow cultures positive for brucella melitensis. thirty patients received 200 mg of doxy ... | 1993 | 8239591 |
| simultaneous expression of smooth and rough phase properties related to lipopolysaccharide in a strain of brucella melitensis. | brucella strains exhibit either a rough (r) or a smooth (s) colonial phase identifiable by bacteriological methods. this depends on the biosynthesis and translocation to the surface in s but not in r strains, of the o-polysaccharide chain of the lipopolysaccharide (lps) molecule. b. melitensis biovar 1 strain ep exhibited simultaneously both s and r characteristics in relation to colonial morphology, agglutination by monospecific anti-m and anti-r sera, activity of bacteriophages lytic for rough ... | 1993 | 8246253 |
| brucellosis of the spine. | the spinal form of brucellosis is still a disabling disease in many countries outside north america and northern and central europe. fifteen consecutive cases of spinal brucellosis diagnosed and treated over a 20-year period were reviewed retrospectively. six patients were farmers, while 10 patients had a history of ingestion of unpasteurized milk or other dairy products. a high index of suspicion is necessary for the diagnosis, since there are no pathognomonic signs or symptoms. radiological as ... | 1993 | 8264880 |
| [brucellosis: case report and synopsis of 10 cases (1973-1992) in the chur canton hospital]. | a 33-year-old patient from former yugoslavia presented with low back pain and fever. plain x-rays and myelography were normal, but a technetium-99m scan was suspect for vertebral osteomyelitis. serology and blood cultures were positive for brucella (melitensis). antibiotic therapy with tetracycline and rifampicine rapidly improved the signs and symptoms of the infection. a retrospective analysis of 11 cases of brucellosis treated in the kantonsspital, chur, over the years 1973 to 1992 revealed t ... | 1993 | 8272813 |
| therapy of experimental murine brucellosis with streptomycin alone and in combination with ciprofloxacin, doxycycline, and rifampin. | the in vivo efficacy of streptomycin (str), doxycycline (dox), rifampin (rif), ciprofloxacin (cip), and their combinations was evaluated for a brucella melitensis experimental infection in a mouse model. animals were infected with 2 x 10(4) to 4 x 10(4) cfu of b. melitensis intraperitoneally on day 0 and were randomized to receive, starting on day 7, str alone at 75, 150, or 300 mg/kg of body weight per day intraperitoneally or dox at 6 mg/kg/day orally, rif at 3 mg/kg/day orally, or cip at 200 ... | 1993 | 8285615 |
| sensorineural hearing loss in neurobrucellosis. | a case of neurobrucellosis presenting to the otologist with sensorineural hearing loss (snhl) as the predominant clinical feature is reported. the diagnostic features and treatment options are discussed and the need for prolonged combination treatment to prevent relapse and further deterioration of hearing stressed. snhl in neurobrucellosis has hitherto been reported principally in neurology literature as something of an incidental finding and so escapes the attention of otologists. it is hoped ... | 1993 | 8288975 |
| high-level expression and purification of secreted forms of herpes simplex virus type 1 glycoprotein gd synthesized by baculovirus-infected insect cells. | two forms of herpes simplex virus glycoprotein gd were recombined into autographa californica nuclear polyhedrosis virus (baculovirus) and expressed in infected spodoptera frugiperda (sf9) cells. each protein was truncated at residue 306 of mature gd. one form, gd-1(306t), contains the coding sequence of patton strain herpes simplex virus type 1 gd; the other, gd-1(qaat), contains three mutations which eliminate all signals for addition of n-linked oligosaccharides. prior to recombination, each ... | 1994 | 8289380 |
| [acute brucellosis, pancytopenia and bone marrow hypoplasia]. | 1993 | 8289516 | |
| brucellosis: differential diagnosis of acute abdominal pain. | a 34-year-old turkish woman presented with septic fever, sweats, arthralgia, and abdominal pain. further examination revealed generalized lymphadenopathy, hepatosplenomegaly, pancytopenia, and multiple caseous granulomas. microbiological diagnosis revealed brucella melitensis type 3 as causative agent. this case report demonstrates that abdominal pain can be a symptom in brucellosis, and caseous granulomas may occur. in our mobile society brucella infection should be considered as possible diffe ... | 1993 | 8291280 |
| southwestern internal medicine conference: brucellosis: don't let it get your goat! | brucellosis is a highly pleomorphic zoonotic infection caused by one of the following four species of gram-negative facultative intracellular coccobacilli: brucella melitensis, b. abortus, b. suis, or b. canis. the disease is a worldwide public health problem and a significant cause of economic losses in domestic live-stock. although largely eradicated in most industrialized countries, in the united states there has been an upsurge of b. melitensis cases associated with the ingestion of unpasteu ... | 1994 | 8291510 |
| efficacy of brucella suis strain 2 vaccine against brucella ovis in rams. | the protective efficacy against brucella ovis of live vaccine brucella suis strain 2 (s2) and brucella melitensis strain rev 1 has been evaluated in rams. fourteen 4-month-old brucella-free aragonesa rams were vaccinated conjunctivally with 2 x 10(9) c.f.u. s2. sixteen rams of the same breed, condition and age were conjunctivally vaccinated the same day with 1.6 x 10(9) rev 1. thirteen rams were unvaccinated controls. eight months after vaccination all rams were challenged with 6 x 10(9) c.f.u. ... | 1993 | 8296481 |
| protective immunity to brucella ovis in balb/c mice following recovery from primary infection or immunization with subcellular vaccines. | experiments were performed with balb/c mice to elucidate the roles of humoral and cell-mediated immune responses in the acquisition of protective immunity to brucella ovis and to compare infection immunity with immunity developed through vaccination with a hot saline extract (hs) of b. ovis. mice convalescing from a primary infection with b. ovis displayed a high level of resistance to reinfection, as evidenced by splenic bacterial counts decreased over 10,000-fold from control groups at 2 weeks ... | 1994 | 8300219 |
| comparative analysis of brucella serotype a and m and yersinia enterocolitica o:9 polysaccharides for serological diagnosis of brucellosis in cattle, sheep, and goats. | hapten polysaccharides of brucella smooth m and a serotypes were prepared from brucella sp. and yersinia enterocolitica o:9 by previously described hydrolytic (o chain) or nonhydrolytic (native hapten [nh]) procedures. the purified polysaccharides differed only in the presence (o chain) or absence (nh) of lipopolysaccharide core sugars. the polysaccharides were compared by reverse radial immunodiffusion for the diagnosis of brucellosis in cattle (brucella abortus biotype 1 [a serotype] and bruce ... | 1993 | 8308104 |
| factors affecting detection of brucella melitensis by bactec nr730, a nonradiometric system for hemocultures. | the detection of brucella bacteremia by subculture does not always correlate with a positive signal in the bactec nr730 nonradiometric system (becton dickinson diagnostic instrument systems, sparks, md.). the effect of the inoculum size, ph, sodium polyanetholesulfonate, carbon sources (i-erythritol, sodium pyruvate, monosodium glutamate, d-glucose, and l-alanine), and urea in the release of co2 was evaluated by using the reference strain brucella melitensis 16m. in standard nr6 vials with or wi ... | 1993 | 8308111 |
| brucellar sacroiliitis: findings in 63 episodes and current relevance. | between 1974 and 1989 we prospectively observed 530 patients with brucellosis. the findings for 62 patients (42 males and 20 females; mean age, 34.7 years) with 63 episodes in which the sacroiliac joint was involved (the most frequent osteoarticular location [11.7%]) were analyzed. ten of the older patients (mean age, 55.3 years) had concomitant spondylitis. systemic symptoms were usually important, and characteristic pain and findings of sacroiliitis were observed in approximately 75% of cases. ... | 1993 | 8329507 |
| persistent coccobacillary sepsis in a preterm newborn. | 1993 | 8345992 | |
| crystal structure to 2.45 a resolution of a monoclonal fab specific for the brucella a cell wall polysaccharide antigen. | the atomic structure of an antibody antigen-binding fragment (fab) at 2.45 a resolution shows that polysaccharide antigen conformation and fab structure dictated by combinatorial diversity and domain association are responsible for the fine specificity of the brucella-specific antibody, yst9.1. it discriminates the brucella abortus a antigen from the nearly identical brucella melitensis m antigen by forming a groove-type binding site, lined with tyrosine residues, that accommodates the rodlike a ... | 1993 | 8358294 |