Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| time-dependent reversal of sepsis-induced pmn uptake and lung vascular injury by expression of cd18 antagonist. | we determined the time-dependent effects of conditional expression of neutrophil inhibitory factor (nif), a specific 41-kda cd18 integrin antagonist, on the time course of nif expression and lung pmn (polymorphonuclear leukocyte) infiltration and vascular injury in a model of escherichia coli-induced sepsis in mice. studies were made in mice transduced with the e-selectin (es) promoter-nif construct (using liposomes) in which the nif cdna was driven by the inflammation- and endothelial cell-spec ... | 2002 | 11880306 |
| delineation of the key amino acids involved in neutrophil inhibitory factor binding to the i-domain supports a mosaic model for the capacity of integrin alphambeta 2 to recognize multiple ligands. | to gain insight into the mechanism by which the alpha(m)i-domain of integrin alpha(m)beta(2) interacts with multiple and unrelated ligands, the identity of the neutrophil inhibitory factor (nif) recognition site was sought. a systematic strategy in which individual amino acid residues within three previously implicated segments were changed to those in the alpha(l)i-domain, which is structurally very similar but does not bind nif, was implemented. the capacity of the resulting mutants, expressed ... | 2002 | 11880366 |
| one confirmed and six suspected cases of cutaneous larva migrans caused by overseas infection with dog hookworm larvae. | cutaneous larva migrans (clm) is characterized as creeping eruption/serpiginous erythema and/or mobile erythematous induration on the skin. in japan, gnathostoma spp. are the most well known pathogens causing clm, especially the creeping eruption type. recently, spirurina type x larvae have been added to the list of causative agents for creeping eruption in japan. here we report one confirmed and 6 suspected cases of creeping eruption caused by infection with dog hookworm larvae. the patients we ... | 2002 | 11890293 |
| a developmentally regulated metalloprotease secreted by host-stimulated ancylostoma caninum third-stage infective larvae is a member of the astacin family of proteases. | 2002 | 11897134 | |
| fenbendazole treatment of pregnant bitches to reduce prenatal and lactogenic infections of toxocara canis and ancylostoma caninum in pups. | a granulated formulation of fenbendazole was tested in a total of 23 treated and control, pregnant, parasite-free beagle bitches experimentally infected with toxocara canis and ancylostoma caninum. the drug was administered to each treated bitch once daily in canned dog food at a dosage of 50 mg/kg body weight. each of 2 treatment regimens tested was initiated on the 40th day of pregnancy. one regimen involved daily treatment continuing through the 14th postpartum day, and it resulted in 89% few ... | 1983 | 12002591 |
| nematode anticoagulant protein c2 reveals a site on factor xa that is important for macromolecular substrate binding to human prothrombinase. | the binding of recombinant nematode anticoagulant protein c2 (napc2) to either factor x or xa is a requisite step in the pathway for the potent inhibition of viia tissue factor. we have used napc2 as a tight binding probe of human xa to investigate protein substrate recognition by the human prothrombinase complex. napc2 binds with high affinity (k(d) approximately 1 nm) to both x and xa in a way that does not require or occlude the active site of the enzyme. in contrast, napc2 is a tight binding ... | 2002 | 12011050 |
| cutaneous larva migrans with parts of the larva in the epidermis. | creeping eruption is usually caused by hookworms, most commonly ancylostoma braziliensis and ancylostoma caninum. because lesions of cutaneous larva migrans have a typical clinical appearance, they are rarely biopsied. specimens usually show spongiotic dermatitis with spongiotic vesicles containing neutrophils and eosinophils and a mixed-cell dermal infiltrate with numerous eosinophils. we report a case with parts of the larva migrans in the epidermis on histologic examination. | 2002 | 12041816 |
| a cathepsin l protease essential for caenorhabditis elegans embryogenesis is functionally conserved in parasitic nematodes. | proteolytic enzymes are involved in processes important to development and survival of many organisms. parasite proteases are considered potential targets of parasite control yet, for most, their precise physiological functions are unknown. validation of potential targets requires analysis of function. we have recently identified a cathepsin l (cpl) cysteine protease, ce-cpl-1, which is essential for embryonic development of the free-living nematode caenorhabditis elegans. we now show that cpl g ... | 2002 | 12076767 |
| evolution of glycoprotein hormone subunit genes in bilateral metazoa: identification of two novel human glycoprotein hormone subunit family genes, gpa2 and gpb5. | the canonical members of the human glycoprotein hormone subunit family of cystine knot-forming polypeptides include the common alpha-subunit, and four beta-subunit genes, fshbeta, lhbeta, tshbeta, and hcgbeta. using pairwise sequence analysis of the complete human genome, we have identified two novel glycoprotein hormone subunit-related genes. based on unique sequence similarity to the alpha- and beta-subunits of glycoprotein hormones, they were named glycoprotein-alpha2 (gpa2) and glycoprotein- ... | 2002 | 12089349 |
| molecular cloning and purification of ac-tmp, a developmentally regulated putative tissue inhibitor of metalloprotease released in relative abundance by adult ancylostoma hookworms. | a cdna encoding a putative tissue inhibitor of metalloprotease was cloned from an ancylostoma caninum adult hookworm cdna library by immunoscreening with anti-hookworm secretory products antiserum. ac-tmp (a. caninum tissue inhibitor of metalloproteinase) is encoded by a 480-bp mrna with a predicted open reading frame of 140 amino acids (molecular weight, 16,100 da) that contains one potential n-linked glycosylation site and an n-terminal cys-x-cys consensus sequence. the open reading frame corr ... | 2002 | 12139214 |
| helminths in dogs from a peri-urban resource-limited community in free state province, south africa. | this study focused on the helminth parasites of dogs in the bloemfontein area of the free state province of south africa. samples were collected from 63 stray dogs destined for euthanasia. complete sets of samples, consisting of blood, faeces, adhesive tape swabs and organs (heart, lungs and complete gastro-intestinal tract) from each dog were examined for helminth parasites. dipylidium caninum was the most common helminth, and was recovered from 44% of dogs, followed by taenia spp. (33%), toxas ... | 2002 | 12163245 |
| effect of vaccinations with recombinant fusion proteins on ancylostoma caninum habitat selection in the canine intestine. | laboratory dogs were vaccinated subcutaneously with 3 different recombinant fusion proteins, each precipitated with alum or calcium phosphate. the vaccinated dogs were then challenged orally with 400 third-stage infective larvae (l3) of the canine hookworm, ancylostoma caninum. the 3 a. caninum antigens selected were ac-tmp, an adult-specific secreted tissue inhibitor of metalloproteases; ac-ap, an adult-specific secreted factor xa serine protease inhibitor anticoagulant; and ac-arr-1, a catheps ... | 2002 | 12197114 |
| influence of parasitic life style on the patterns of codon usage and base frequencies of ancylostoma and necator species. | parametric analyses were used to investigate the nucleotide, codon, and amino acid composition of coding sequences corresponding to hook-worms. ancylostoma caninum and necator americanus. although genomic research has become prevalent within the scientific community, few studies have dealt directly with parasitic species. parasites have existed throughout the history of mankind due to their wide range of distribution in nature and their ability to evade immune detection. an at nucleotide bias wa ... | 2002 | 12214942 |
| improved detection of endoparasite dna in soil sample pcr by the use of anti-inhibitory substances. | although there have been numerous microbial examinations of soil for the presence of human pathogenic developmental parasite stages of ancylostoma caninum and toxocara canis, molecular techniques (e.g. dna extraction, purification and subsequent pcr) have scarcely been applied. here, dna preparations of soil samples artificially contaminated with genomic dna or parasite eggs were examined by pcr. a. caninum and t. canis-specific primers based on the its-2 sequence were used for amplification. af ... | 2002 | 12237140 |
| recombinant nematode anticoagulant protein c2, a novel inhibitor of tissue factor-factor viia activity, abrogates endotoxin-induced coagulation in chimpanzees. | systemic activation of coagulation leading to disseminated intra-vascular coagulation (dic) is an important feature in patients with severe sepsis. tissue factor has been shown to play a primary role in this pathological response, as revealed by the use of specific inhibitors and antagonists of the tissue factor/factor viia pathway. this class of agents has been demonstrated to attenuate the coagulation response in human volunteers with induced low-grade endotoxemia and to reduce mortality in pr ... | 2002 | 12362234 |
| a novel cathepsin b active site motif is shared by helminth bloodfeeders. | this study compared specific protein sequence motifs present within cathepsin b-like cysteine proteases from a number of helminth parasites. we have focused our efforts on cathepsin b-like proteases of haemonchus contortus, caenorhabditis elegans, schistosoma mansoni, schistosoma japonicum, ostertagia ostertagi, and ancylostoma caninum. the goal of this work is to correlate specific features, or proposed roles, of the cathepsin b-like proteases with primary sequence motifs discovered within the ... | 2002 | 12427461 |
| characterization of a recombinant immunodiagnostic antigen (nie) from strongyloides stercoralis l3-stage larvae. | due to the process of internal autoinfection, even chronic asymptomatic infections with strongyloides stercoralis have the potential to become severe disseminated disease with fatal outcome. intermittent and scanty larval excretion makes parasitologic diagnosis difficult. serodiagnosis is helpful, but antigen preparation from infective larvae requires access to patients or immunosuppressed experimental animals. for these reasons, attention has turned to recombinant antigens for immunodiagnosis. ... | 2002 | 12467975 |
| a novel repressor of nif and glna expression in the methanogenic archaeon methanococcus maripaludis. | nitrogen assimilation in the methanogenic archaeon methanococcus maripaludis is regulated by transcriptional repression involving a palindromic 'nitrogen operator' repressor binding sequence. here we report the isolation of the nitrogen repressor, nrpr, from m. maripaludis using dna affinity purification. deletion of the nrpr gene resulted in loss of nitrogen operator binding activity in cell extracts and loss of repression of nif (nitrogen-fixation) and glna (glutamine synthetase) gene expressi ... | 2003 | 12492867 |
| experimental human infection with the dog hookworm, ancylostoma caninum. | to investigate possible routes for human infection by the dog hookworm (ancylostoma caninum). design, setting and participant: relatively small numbers of infective larvae were administered orally and percutaneously to an informed healthy volunteer (j k l) under medical supervision, at intervals between may 1998 and may 1999. | 2003 | 12526725 |
| inhibition of factor viia/tissue factor with nematode anticoagulant protein c2: from unique mechanism to a promising new clinical anticoagulant. | recombinant nematode anticoagulant protein c2 (rnapc2) is a potent (k(i) = 10 pm) inhibitor of the factor viia/tissue factor complex (fviia/tf) that involves the pre-requisite binding to either zymogen or activated factor x (fx) prior to the formation of the final quaternary complex with fviia/tf. the formation of the binary complex with circulating fx governs the pharmacokinetic profile of rnapc2 in humans, resulting in a prolonged elimination half-life of >50 h. the clinical antithrombotic pot ... | 2002 | 12536118 |
| prevalence of enteric pathogens in dogs of north-central colorado. | to evaluate the prevalence of enteric pathogens in dogs of north-central colorado, fecal samples were obtained from client-owned dogs presented to the veterinary teaching hospital at colorado state university for evaluation of acute small-bowel, large-bowel, or mixed-bowel diarrhea (n=71) and from age-matched, client-owned, healthy dogs (n=59). infectious agents potentially associated with gastrointestinal disease were detected in 34 of 130 (26.1%) fecal samples. agents with zoonotic potential w ... | 2003 | 12549614 |
| vertical migration by the infective larvae of three species of parasitic nematodes: is the behaviour really a response to gravity? | vertical migration by infective larvae (l3) of 3 species of nematodes was investigated. upright truncated agarose cones were used to test upward migration, and comparable truncated cone-shaped agarose hollows were used to test downward migration. flat agarose plates were control surfaces. when placed at the bases of upright cones, 74% of ancylostoma caninum l3 migrated up, whereas only 16.5% migrated down the indented cones; this latter value was not significantly different from larval migration ... | 2002 | 12553574 |
| ac-far-1, a 20 kda fatty acid- and retinol-binding protein secreted by adult ancylostoma caninum hookworms: gene transcription pattern, ligand binding properties and structural characterisation. | antibody against adult ancylostoma caninum excretory-secretory (es) products was used to immunoscreen a cdna expression library leading to the isolation of cdnas encoding putative hookworm fatty-acid and retinol-binding proteins. ac-far-1 and ac-far-2 cdnas encode open reading frames corresponding to approximately 20kda proteins with 91 percent amino acid identity. ac-far-1 and ac-far-2 exhibit clear similarities to other fars of parasitic nematodes, most closely to two of the far proteins of ca ... | 2003 | 12554085 |
| evaluation of the role of ancylostoma caninum in humans as a cause of acute and recurrent abdominal pain. | ancylostoma caninum is responsible for cases with eosinophilic enteritis (ee) and unexplained abdominal pain with peripheral eosinophilia in man. ninety-five patients with obscure acute or recurrent abdominal pain and ten asymptomatic healthy parasite free were subjected to thorough history taking, clinical examination, sonography, routine laboratory investigations and serotesting by igg elisa to detect antibodies to excretory/secretory (es) antigens of adult a. caninum and by igg and igg4 weste ... | 1999 | 12561926 |
| effect of lead and ancylostomiasis on phosphatases of heart in swiss albino mice. | administration of 0.01 and 0.1 mg of lead nitrate for 4 and 7 days and infection of ancylostoma caninum larvae orally altered the activation of alkaline phosphatase and acid phosphatase in the hearts of mice when compared to infected animals and controls. alkaline phosphatase activity increased significantly in all drug-treated + infected mice. the level of acid phosphatase decreased significantly in mice exposed to chronic doses of lead. the altered levels of alkaline phosphatase and acid phosp ... | 2002 | 12568465 |
| [studies on immunological reaction of the antiserum of recombinant secreted protein from ancylostoma caninum]. | to analyze the immunological reaction of the antiserum of recombinant secreted protein from ancylostoma caninum with antigens of various species hookworms at different developmental stages. | 2001 | 12571945 |
| characterisation of humoral immune responses in dogs vaccinated with irradiated ancylostoma caninum. | infection with ancylostoma caninum, an intestinal hookworm of dogs, can cause debilitating and potentially life-threatening disease. in the current study, protective immunity to hookworm infection was induced in dogs following vaccination with irradiation-attenuated third-stage larvae (l3) with significant reductions in both worm (p<0.03) and faecal egg counts (p<0.0004) following a challenge infection. vaccination with irradiated l3 and challenge with infective l3 stimulated a dominant antibody ... | 2003 | 12628766 |
| hookworm cathepsin d aspartic proteases: contributing roles in the host-specific degradation of serum proteins and skin macromolecules. | cathepsin d aspartic proteases of hookworms were recently implicated in the host-specific digestion of haemoglobin by adult parasites. ac-apr-1 from the dog hookworm, ancylostoma caninum and na-apr-1 from the human hookworm, necator americanus, were shown to be expressed in the infective larval stage (l3) as well as adult worms. we now show that both proteases degraded skin macromolecules and serum proteins, some of which were cleaved more readily from permissive definitive hosts as opposed to n ... | 2003 | 12636356 |
| activation of coagulation by administration of recombinant factor viia elicits interleukin 6 (il-6) and il-8 release in healthy human subjects. | the activation of coagulation has been shown to contribute to proinflammatory responses in animal and in vitro experiments. here we report that the activation of coagulation in healthy human subjects by the administration of recombinant factor viia also elicits a small but significant increase in the concentrations of interleukin 6 (il-6) and il-8 in plasma. this increase was absent when the subjects were pretreated with recombinant nematode anticoagulant protein c2, the inhibitor of tissue fact ... | 2003 | 12738659 |
| molecular cloning of a novel multidomain kunitz-type proteinase inhibitor from the hookworm ancylostoma caninum. | degenerate oligonucleotide primers derived from conserved serine protease inhibitors were used to amplify a 90-base pair (bp) amplicon from an ancylostoma caninum adult-stage complementary deoxyribonucleic acid (cdna) library by polymerase chain reaction (pcr). the amplicon was labeled and used as a probe to screen the library, and a 2,300-bp cdna clone was identified. the 5' end of the molecule was obtained from adult cdna by 5'-race. the complete sequence named a. caninum kunitz-type protease ... | 2003 | 12760667 |
| tissue factor/factor viia inhibitors block angiogenesis and tumor growth through a nonhemostatic mechanism. | an association between cancer and thrombosis has been recognized for more than a century. however, the manner by which tumor growth is regulated by coagulation in vivo remains unclear. to assess the role of coagulation on tumor growth, in vivo, we tested coagulation inhibitors specific for either tissue factor (tf)/factor viia (fviia) complexes or factor xa (fxa) for antitumor activity. here, we show that two inhibitors of tf/fviia, tf pathway inhibitor (tfpi) and the nematode anticoagulant prot ... | 2003 | 12782609 |
| uk-279,276, a neutrophil inhibitory glycoprotein, in acute stroke: tolerability and pharmacokinetics. | uk-279,276, a recombinant glycoprotein, binds selectively to the cd11b/cd18 integrin on neutrophils and has the potential to modulate the neuroinflammation associated with acute stroke. after preclinical evidence of neuroprotection, uk-279,276 has entered clinical development. the purposes of this study were to evaluate the safety and tolerability of uk-279,276 and to examine its pharmacokinetics and pharmacodynamics (binding to neutrophil cd11b) in patients with acute stroke. | 2003 | 12805489 |
| effects of a selective cd11b/cd18 antagonist and recombinant human tissue plasminogen activator treatment alone and in combination in a rat embolic model of stroke. | we evaluated the neuroprotective effect of uk-279,276 (also referred to as recombinant neutrophil inhibitory factor), a selective cd11b/cd18 antagonist, in combination with thrombolytic therapy on focal cerebral ischemia. | 2003 | 12805500 |
| e. coli pneumonia induces cd18-independent airway neutrophil migration in the absence of increased lung vascular permeability. | we examined the relationship between neutrophil [polymorphonuclear leukocyte (pmn)] influx and lung vascular injury in response to escherichia coli pneumonia. we assessed lung tissue pmn uptake by measuring myeloperoxidase and transvascular pmn migration by determining pmn counts in lung interstitium and bronchoalveolar lavage fluid (balf) in mice challenged intratracheally with e. coli. lung vascular injury was quantified by determining microvessel filtration coefficient (kf,c), a measure of va ... | 2003 | 12818890 |
| recombinant nematode anticoagulant protein c2, an inhibitor of the tissue factor/factor viia complex, in patients undergoing elective coronary angioplasty. | we investigated the safety and pharmacodynamics of escalating doses of recombinant nematode anticoagulant protein c2 (rnapc2) in patients undergoing elective coronary angioplasty. | 2003 | 12821239 |
| isolation and molecular cloning of a secreted hookworm platelet inhibitor from adult ancylostoma caninum. | hookworms, bloodfeeding intestinal nematodes, are a leading cause of iron deficiency anemia in the developing world. these parasites have evolved potent mechanisms of interfering with mammalian hemostasis, presumably for the purpose of facilitating bloodfeeding. adult ancylostoma caninum worm extracts contain an activity that inhibits platelet aggregation and adhesion by blocking the function of two cell surface integrin receptors, glycoprotein iib/iiia and gpia/iia. using rphplc, the hookworm p ... | 2003 | 12850261 |
| eosinophil relationship in gut anaphylaxis during experimental ancylostomosis. | changes induced in the circulating and intestinal eosinophil levels and worm load were studied in female swiss albino mice infected with multiple doses of ancylostoma caninum larvae. there was a significant rise of both circulating and intestinal eosinophils in all the infected animals when compared with controls. in all the experimental groups of animals, peak eosinophilia was observed during the third week of post-infection and sustained upto 30th day with slight alterations. these studies ind ... | 2003 | 12860065 |
| the second messenger cyclic gmp mediates activation in ancylostoma caninum infective larvae. | the developmentally arrested infective larva (l(3)) of hookworms encounters a host-specific signal during infection that initiates previously suspended developmental pathways. activated l(3) express a parasitic gene set that encodes proteins involved in moulting, growth and development to the adult stage. early events in this activation to parasitism can be investigated using an in vitro larval feeding assay. when ancylostoma caninum l(3) are exposed to a host-like stimulus, they resume feeding ... | 2003 | 12865078 |
| [cloning and expression of specific antigen genes of ancylostoma caninum]. | to search for the gene encoding specific antigen of ancylostoma caninum that induces host's protective immunity. | 2003 | 12884580 |
| [helminthes infections in the small intestine of stray dogs in ejungbu city, kyunggi do, korea] | one hundred and two stray dogs in ejungbu city of kyunggi-do, korea were examined to reveal out the degree of natural helminthic infection of small intestine. helminthes were collected at autopsy, by scraping the intestinal contents. the collected worms were classified by their morphological characteristics. out of 102 examined, 72 dogs were infected with any helminthes. the common helminthes were dipylidium caninum (47%), ancylostoma caninum (26%), toxascaris leonina (16%) and toxocara canis (1 ... | 1981 | 12902719 |
| [an epidemiological study on zoonoses in korea] | helminthic zoonoses are of significant importance to the public health and to the socioeconomic consequences especially in lost-meat, animal products and animal labor. for past several decades in korea, it has been recognized that endoparasitic infections among domestic animals are extremely common and many of the helminth parasites of man have been acquired from, or are shared with domestic animals. however, there was no survey of a nationwide scale on the prevalence of animal helminthiasis; an ... | 1981 | 12902720 |
| molecular characterisation of the ancylostoma-secreted protein family from the adult stage of ancylostoma caninum. | the ancylostoma-secreted proteins are a family of nematode-specific cysteine-rich secreted proteins belonging to the pathogenesis-related protein superfamily. previously we reported that third stage infective larvae of ancylostoma caninum produce two different ancylostoma-secreted proteins, a single and double-domain ancylostoma-secreted protein, designated as ancylostoma-secreted protein-1 and ancylostoma-secreted protein-2, respectively. here we report that adult a. caninum hookworms produce a ... | 2003 | 12906874 |
| [the influence of ultraviolet irradiation upon the development and infectivity of hookworm larvae] | the eggs and rhabditoid larvae of canine hookworm were irradiated with ultraviolet rays for one hour at a distance of 10, 20, 30, and 40 cm. the infective stage larvae of the same parasites were irradiated for l, 3, 5 and 14 hours from the same distances. the infective larvae were also exposed under direct sunlight for l, 2, 3 and 4 hours. parasites: ancylostoma caninum was used. eggs were collected in vitro from female adult worms. the worms were kept at 37 degrees c in petri-dish filled with k ... | 1968 | 12913550 |
| [studies on the transmissibility of pathogenic-organisms to liver by migrating larvae of liver fluke and hookworm] | in order to confirm whether the migrating larvae of parasites could carry pathogenic organisms into liver and cause hepatitis, a series of experiments has been carried out. a. clonorchis sinensis: recovery rate of larvae in the abdominal cavity of rabbits: one to seven days after the administration adolescariae were recovered from the abdominal cavity in less than l % of the total number of metacercariae given. generally, 1~6 larvae were found from each animal which was given 900~1,000 metacerca ... | 1968 | 12913551 |
| acquired resistance in mice to the dog hookworm, ancylostoma caninm. | the present study attempted to induce an acquired immunity against the dog hookworm, ancylostoma caninum. the experiments were carried out to demonstrate and confirm whether an acquired resistance could be developed by the repeated lowgrade infections with the infective larvae of a. caninum in the abnormal host of mouse. 1. in order to determined the distribution of hookworm larvae in the lungs and liver of mice after inoculation of infective larvae, 54 mice were inoculated with 1,000 larvae and ... | 1967 | 12913562 |
| [experimental studies on the effects in helminthic infection, with special reference to the gonadectomy of the host] | introduction: concerning the influences of the host's hormone in helminthic infection, solomon(1963) reported that castrated hamsters were less susceptible to nippostrongylus brasiliensis infection than the sham-operated or normal control groups, and addis(1946) demonstrated that testosterone in the male host was necessary for normal growth of hymenolepis diminuta and the egg productive ability was also effected by the hormone. beck(1950, 1952) noted a significant reduction in egg production in ... | 1966 | 12913569 |
| cutaneous larva migrans: the creeping eruption. | cutaneous larva migrans (clm) is the most common tropically acquired dermatosis. it is caused by hookworm larvae, which are in the feces of infected dogs and cats. the condition occurs mainly in the caribbean and new world, and anyone walking barefoot or sitting on a contaminated beach is at risk. ancylostoma braziliense and ancylostoma caninum are the most common hookworms responsible for clm. the lesions, called creeping eruptions, are characteristically erythematous, raised and vesicular, lin ... | 2003 | 12953933 |
| recombinant nematode anticoagulant protein c2 and other inhibitors targeting blood coagulation factor viia/tissue factor. | originally isolated from a haematophagous hookworm, recombinant nematode anticoagulant protein c2 (rnapc2) is an 85-amino acid protein with potent anticoagulant properties. unlike conventional anticoagulants that attenuate blood coagulation via inhibition of thrombin or activated factor x (fxa) at the downstream portion of the cascade, rnapc2 is a potent inhibitor of the activated factor vii/tissue factor complex (fviia/tf), the key physiological initiator of blood coagulation. its mechanism of ... | 2003 | 12974870 |
| [effect of roussin's salt ([fe4s3no7]me) on eggs and rhabditic larvae of ancylostoma caninum]. | 1952 | 12994644 | |
| differentiation of the ova of ancylostoma caninum and uncinaria stenocephala in dogs. | 1953 | 13092396 | |
| the effect of certain chemicals on the eggs and larvae of the canine hookworm (ancylostoma caninum). | 1954 | 13148484 | |
| proteolytic activity in extracts of the esophagus of adults of ancylostoma caninum and the effect of immune serum on this activity. | 1956 | 13295886 | |
| the effect of extracts of the amphidial glands, excretory glands, and esophagus of adults of ancylostoma caninum on the coagulation of dog's blood. | 1956 | 13295887 | |
| continuous phenothiazine therapy of dogs artificially infected with ancylostoma caninum. | 1956 | 13302654 | |
| the etiology of visceral larva migrans. ii. comparative larval morphology of ascaris lumbricoides, necator americanus, strongyloides stercoralis and ancylostoma caninum. | 1956 | 13346428 | |
| survival of ancylostoma caninum in vitro. i. | 1956 | 13415770 | |
| the anthelmintic effect of bephenium on ancylostoma caninum. | 1958 | 13621319 | |
| the ultrastructure of the intestinal wall of ancylostoma caninum. | 1959 | 13665457 | |
| the effect of ancylostoma caninum infection on erythropoiesis in dogs. | 1961 | 13754611 | |
| comparative morphology of ancylostoma tubaeforme (zeder, 1800) and ancylostoma caninum (ercolani, 1859). | 1962 | 14017192 | |
| mating of ancylostoma caninum in relation to blood loss in the host. | 1964 | 14170767 | |
| [the puma (felis concolor) as a new host for ancylostoma caninum (ercolani, 1859) linstow 1889]. | 1964 | 14174786 | |
| [basic studies on parasiticides. 16. insecticidal effects of an azobenzene derivative on ancylostoma caninum larvae in vitro and its parasiticidal effect on ancylostoma caninum]. | 1964 | 14191985 | |
| metabolism of hookworms. ii. glucose metabolism and glycogen synthesis in adult female ancylostoma caninum. | 1964 | 14201360 | |
| in vitro studies of larvicidal agents against the larvae of ancylostoma caninum. | 1964 | 14209495 | |
| studies on the life history of the hookworms. 8. the morphological studies on the development of the 3rd stage larvae of ancylostoma caninum in the normal host. | 1964 | 14225788 | |
| studies on the life history of the hookworms. ix. the morphological studies on the development of the 4th stage larvae of ancylostoma caninum in the normal host. | 1964 | 14225792 | |
| effect of x-irradiation upon the infective larvae of ancylostoma caninum and the immunogenic effect in dogs of a single infection with 40 kr-irradiated larvae. | 1964 | 14244804 | |
| studies on canine ancylostomiasis: double vaccination with x-irradiated ancylostoma caninum larvae. | 1965 | 14256948 | |
| effect of route of administration of vaccine and challenge on the immunogenic efficiency of double vaccination with irradiated ancylostoma caninum larvae. | 1965 | 14275207 | |
| in vitro studies on ancylostoma caninum with thiabendazole with observations on larval exsheathment. | 1965 | 14282469 | |
| [anatomical studies on the development of ancylostoma caninum]. | 1961 | 14457904 | |
| effects of crowding and of superinfection on habitat selection and egg production in ancylostoma caninum. | 1961 | 14459947 | |
| [histochemical studies on ancylostoma caninum. (i-ii)]. | 1961 | 14477283 | |
| electroesophagogram of individual hookworm (ancylostoma caninum). | a device for recording regular rhythmic electrical phenomena that are synchronous with esophageal contractions in ancylostoma cantinum is described. | 1962 | 14493038 |
| evaluation of the role of the asialoglycoprotein receptor in the clearance of uk-279,276 (recombinant neutrophil inhibitory factor). | 1. uk-279,276 or recombinant neutrophil inhibitory factor (rnif) is a glycoprotein that has been investigated as an intravenous treatment for stroke. previous data indicate that the asialoglycoprotein receptor (asgpr) in the liver might have a role in the uptake and clearance of uk-279,276 from plasma in rats and dogs. 2. biliary elimination of sialo uk-279,276 can be inhibited by the co-administration of asialo fetuin, a known substrate for asgpr. these data are in keeping with sialo uk-279,276 ... | 2003 | 14514443 |
| effect of vaccination with a recombinant fusion protein encoding an astacinlike metalloprotease (mtp-1) secreted by host-stimulated ancylostoma caninum third-stage infective larvae. | laboratory dogs were vaccinated intramuscularly with a recombinant fusion protein (expressed and isolated from escherichia coli) formulated with the glaxo smithkline adjuvant system 02 (as02). the fusion protein encoded ac-mtp-1, a developmentally regulated astacinlike metalloprotease secreted by host-stimulated ancylostoma caninum third-stage larvae (l3). control dogs were injected intramuscularly with an equivalent amount of as02 adjuvant alone. the vaccinated and control dogs were then challe ... | 2003 | 14533704 |
| ecological analyses of the intestinal helminth communities of the wolf, canis lupus, in spain. | this work describes the ecological characteristics of the intestinal helminth communities of 50 wolves (canis lupus l.) from spain. the species found were classified into three groups according to prevalence, intensity and intestinal distribution. taenia hydatigena pallas, 1766 and uncinaria stenocephala (railliet, 1884) are the core species of the community. taenia multiceps (leske, 1780) is a secondary species. the rest of the species, alaria alata (goeze, 1782), taenia serialis (gervais, 1847 ... | 2003 | 14535350 |
| haemonchus contortus: molecular characterisation of a small heat shock protein. | a cdna encoding a predicted small heat shock protein, hsp20, was isolated from the parasitic nematode haemonchus contortus. this cdna encoded a predicted protein of 156 amino acids, which had high sequence identity with other nematode small heat shock proteins. southern blot analysis of genomic dna suggested that in h. contortus hsp20 is encoded by a single copy gene. the hsp20 transcript and protein were expressed in the infective larvae (l3), early l4 and adult stages, but expression was not i ... | 2003 | 14552856 |
| [biology and physiopathology of tissue factor and its relevance for cardiovascular diseases]. | tissue factor (tf) is a transmembrane glycoprotein, currently considered as being the major regulator of the coagulation cascade and the initiator of thrombogenesis in vivo. when tf comes in contact with blood, it forms a high-affinity complex with factors vii/viia, activating factors ix and x and thus leading to the formation of an insoluble fibrin clot. the regulation of tf-viia activity plays a key role in blood-vessel wall interactions. selective patterns of cellular expression of tf are obs ... | 2003 | 14558284 |
| pharmacokinetics and anticoagulant properties of the factor viia-tissue factor inhibitor recombinant nematode anticoagulant protein c2 following subcutaneous administration in man. dependence on the stoichiometric binding to circulating factor x. | recombinant nematode anticoagulant protein c2 (rnapc2) is a potent (k(i) =10 pm), inhibitor of the factor viia/tissue factor (fviia/tf) complex that requires the prerequisite binding to zymogen or activated factor x (fx). in two double blind, place-bo-controlled, sequential dose-escalation phase i studies, rnapc2 was found to be safe and well tolerated following single and repeat subcutaneous administrations in healthy human male volunteers at doses ranging from 0.3 to 5 micro g/kg. there was a ... | 2003 | 14597974 |
| virology. new vaccine and treatment excite ebola researchers. | 2003 | 14615510 | |
| treatment of ebola virus infection with a recombinant inhibitor of factor viia/tissue factor: a study in rhesus monkeys. | infection with the ebola virus induces overexpression of the procoagulant tissue factor in primate monocytes and macrophages, suggesting that inhibition of the tissue-factor pathway could ameliorate the effects of ebola haemorrhagic fever. here, we tested the notion that blockade of fviia/tissue factor is beneficial after infection with ebola virus. | 2003 | 14683653 |
| anticoagulant peptides from ancylostoma caninum are immunologically distinct and localize to separate structures within the adult hookworm. | 2004 | 14698444 | |
| a serologically diagnosed human case of cutaneous larva migrans caused by ancylostoma caninum. | a 15-year-old boy, who had recently arrived back from a trip to cambodia for a missionary camp, presented with several serpiginous thread-like skin lesions that began as small papules on the left upper extremities 2 weeks before his visit to hospital. the skin lesions were pruritic and erythematous, and had migrated to the chest and abdomen. the histopathological findings showed only lymphocytic and eosinophilic infiltrations in the dermis of the biopsied skin lesion. the patient's serum reacted ... | 2003 | 14699264 |
| recombinant nematode anticoagulant protein c2, an inhibitor of tissue factor/factor viia, attenuates coagulation and the interleukin-10 response in human endotoxemia. | the tissue factor-factor (f)viia complex (tf/fviia) is responsible for the initiation of blood coagulation under both physiological and pathological conditions. recombinant nematode anticoagulant protein c2 (rnapc2) is a potent inhibitor of tf/fviia, mechanistically distinct from tissue factor pathway inhibitor. the first aim of this study was to elucidate the pharmacokinetics and pharmacodynamics of a single intravenous (i.v.) dose of rnapc2. the second aim was to study its effect on endotoxin- ... | 2004 | 14717968 |
| chemical control of larvae of the dog hookworm ancylostoma caninum (ercolani). | 1951 | 14819522 | |
| recombinant factor viia as an antidote for anticoagulant treatment. | increasing knowledge on the function of the hemostatic system in vivo and limitations of currently available anticoagulant agents have led to the development of a new generation of anticoagulants. these new agents have a greater specificity towards activated coagulation pathways and factors and are presently being evaluated in clinical studies. the new generation anticoagulants include specific inhibitors of factor iia (melagatran), factor xa (pentasaccharides), and agents that interfere with ti ... | 2004 | 14872424 |
| infections of ancylostoma caninum in abnormal hosts. | 1952 | 14928159 | |
| investigations of hookworm larvae. i. on the phototropism of infective larvae of ancylostoma caninum. | 1952 | 14951409 | |
| parasitic helminths of black bear cubs (ursus americanus) from florida. | twenty-two florida black bear (ursus americanus floridanus) cubs (< or = 12 mo old) from florida were examined for endoparasites between 1998 and 2003. eleven species of helminths were found (8 nematodes, 2 trematodes, and 1 acanthocephalan). the most prevalent helminths were ancylostoma caninum (64%), macracanthorhynchus ingens (36%), strongyloides sp. (27%), baylisascaris transfuga (23%), and a. tubaeforme (14%). new host records include a. tubaeforme and brachylaima virginianum. this is the f ... | 2004 | 15040687 |
| prevalence of dogs with intestinal parasites in tochigi, japan in 1979, 1991 and 2002. | a total of 772 canine fecal specimens were collected in tochigi in 1979, 1991 and 2001. the frequency of dogs that were kept indoor in 2002 (72.8%) was significantly higher than that for 1979 (21.2%, p < 0.001) and 1991 (40.6%, p < 0.01). the frequency of parasitized dogs in 2002 (18.8%) was significantly lower than that for 1979 (41.9%, p < 0.01). the findings of this study confirm the declining trend in japan in the prevalence of helminth parasites in dogs, particularly ancylostoma caninum and ... | 2004 | 15041099 |
| rnapc2. nuvelo. | recombinant nematode anticoagulant protein-2 (rnapc2) is an antithrombotic protein under development by nuvelo as a potential therapeutic agent for the treatment of major acute cardiovascular disease such as unstable angina and myocardial infarction. phase ii clinical trials were ongoing in january 2004, although development for deep vein thrombosis had been suspended by dendreon due to a requirement for a partner to assist with additional phase ii clinical trials. | 2004 | 15083600 |
| vaccination of dogs with a recombinant cysteine protease from the intestine of canine hookworms diminishes the fecundity and growth of worms. | we expressed a catalytically active cysteine protease, ac-cp-2, from the blood-feeding stage of the canine hookworm ancylostoma caninum and vaccinated dogs with the purified protease. dogs acquired high-titer, antigen-specific antibody responses, and adult hookworms recovered from the intestines of vaccinated dogs were significantly smaller than hookworms from control dogs. there was also a marked decrease in fecal egg counts and the number of female hookworms in vaccinated dogs. ac-cp-2 is expr ... | 2004 | 15122534 |
| persistent efficacy of moxidectin canine sustained-release injectable against experimental infections of ancylostoma caninum and uncinaria stenocephala in dogs. | the efficacy of moxidectin canine sustained-release injectable administered at fixed intervals before administration of an oral hookworm challenge was evaluated in 40 laboratory dogs. groups of eight dogs were treated with the moxidectin sustained-release formulation by sc injection approximately 3, 4, 5, or 6 months before being given oral inoculations with 300 ancylostoma caninum larvae on day 0 and 400 uncinaria stenocephala larvae one day later. dogs were euthanized 21 days after parasite in ... | 2003 | 15136983 |
| inhibition of the tissue factor/factor viia pathway does not influence the inflammatory or antibacterial response to abdominal sepsis induced by escherichia coli in mice. | anticoagulants have gained increasing attention for the treatment of sepsis. inhibition of the tissue factor (tf)/factor (f) viia pathway has been shown to attenuate the activation of coagulation and to prevent death in a primate model of sepsis caused by gram-negative bacteria. | 2004 | 15181580 |
| a multi-enzyme cascade of hemoglobin proteolysis in the intestine of blood-feeding hookworms. | blood-feeding pathogens digest hemoglobin (hb) as a source of nutrition, but little is known about this process in multicellular parasites. the intestinal brush border membrane of the canine hookworm, ancylostoma caninum, contains aspartic proteases (apr-1), cysteine proteases (cp-2), and metalloproteases (mep-1), the first of which is known to digest hb. we now show that hb is degraded by a multi-enzyme, synergistic cascade of proteolysis. recombinant apr-1 and cp-2, but not mep-1, digested nat ... | 2004 | 15199048 |
| phosphoinositide-3-oh-kinase inhibitor ly294002 prevents activation of ancylostoma caninum and ancylostoma ceylanicum third-stage infective larvae. | the developmentally arrested hookworm infective larva resumes development only after encountering specific host-mediated cues during invasion. these cues activate a signaling pathway that culminates in the resumption of development. in ancylostoma caninum, activation is characterised by the resumption of feeding and the release of excretory/secretory products required for infection. the dauer stage of the free-living nematode caenorhabditis elegans is a developmentally arrested stage analogous t ... | 2004 | 15217729 |
| intestinal obstruction by eosinophilic jejunitis. | eosinophilic enteritis is an uncommon disease that rarely develops as a surgical emergency. although it may be associated with infestation by ancylostoma caninum, its etiology is unknown and often related to a personal or family history of atopy. a transmural involvement may cause intestinal obstruction--more frequently in the jejunum--or even acute abdomen, which may or may not be accompanied by intestinal perforation. the latter two conditions tend to be more commonly associated with ileum dis ... | 2004 | 15259143 |
| ancylostoma ceylanicum anticoagulant peptide-1: role of the predicted reactive site amino acid in mediating inhibition of coagulation factors xa and viia. | hookworm infection is a leading cause of gastrointestinal blood loss and iron deficiency anemia in developing countries. ancylostoma hookworms secrete potent anticoagulants, which have been shown to target coagulation factors xa and the factor viia/tissue factor complex, respectively. the goal of these experiments was to determine the mechanism of action of three recombinant hookworm anticoagulants using in vitro assays. three hookworm coagulation inhibitors were expressed and purified, along wi ... | 2004 | 15279961 |