Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| effects of bovine respiratory syncytial virus on airway function in neonatal calves. | respiratory syncytial virus (rsv) infection causes severe lower respiratory tract disease in infants and calves. neonatal respiratory tract infection in children often produces persistent changes in lung function. the specific objective of this study was to determine whether neonatal calves have transient or persistent alterations in pulmonary function and airway reactivity following rsv infection. six 2- to 3-day-old holstein bull calves were inoculated with 10 ml of bovine respiratory syncytia ... | 1991 | 1952323 |
| purification and properties of extracellular matrix-degrading metallo-proteinase overproduced by rous sarcoma virus-transformed rat liver cell line, and its identification as transin. | rous sarcoma virus-transformed rat liver cell line rsv-brl secreted a neutral proteinase in a latent precursor form with a molecular weight (mr) of 57,000 (57k) as a major secreted protein. this enzyme was a calcium-dependent metallo-proteinase. the proenzyme was purified from the serum-free conditioned medium of the transformed cells by affinity chromatographies on a zinc chelate sepharose column and a reactive red agarose column. when activated by treatment with trypsin or p-aminophenylmercuri ... | 1990 | 1963430 |
| viruses associated with acute lower respiratory tract infections in children from the eastern highlands of papua new guinea (1983-1985). | this study, conducted at goroka hospital from january 1983 to june 1985, examined the viruses identified in nasopharyngeal aspirates (npa) and urines collected from 716 hospitalised children with moderate or severe pneumonia, in npa from 170 children with mild pneumonia treated as outpatients and in npa from a control group of 428 children attending the outpatient department of goroka hospital suffering from minor ailments other than upper or lower respiratory tract infections. one or more virus ... | 1990 | 1963705 |
| evaluation of the toxicity and antiviral activity of carbocyclic 3-deazaadenosine against respiratory syncytial and parainfluenza type 3 viruses in tissue culture and in cotton rats. | the toxicity and antiviral efficacy of carbocyclic 3-deazaadenosine (cc3ado) against respiratory syncytial (rsv) and parainfluenza type 3 (piv3) virus infections were tested in tissue culture and in cotton rats. the mean median efficacious dose (ed50) of cc3ado in hep2 cells against rsv and piv3 was 9 and 14 micrograms/ml, respectively. these values were 85- and 55-fold less than the median inhibitory (toxic) dose (id50) of cc3ado in this cell line (750 micrograms/ml), and similar to values obta ... | 1990 | 1965109 |
| toxicity and antiviral activity of ly253963 against respiratory syncytial and parainfluenza type 3 viruses in tissue culture and in cotton rats. | ly253963, the sodium salt of 1,3,4-thiadiazol-2-ylcyanamide, was evaluated in tissue culture and in cotton rats for toxicity and antiviral efficacy against respiratory syncytial (rsv) and parainfluenza type 3 (piv3) viruses. the selective index (ratio of the median toxic dose: median efficacious dose) of ly253963 in hep2 tissue culture cells was greater than 100 against both rsv and piv3. when given intraperitoneally to cotton rats, the minimum protective dose of ly253963 against both of these v ... | 1990 | 1965110 |
| transmembrane interactions at cell adhesion and invasion sites. | chicken embryonic fibroblasts transformed by rous sarcoma virus (rsv-cef) invade into a film of the extracellular matrix (ecm) by extending membrane protrusions, termed the invadopodia. the invadopodia share similar cytoskeletal components and membrane receptors for ecm components as adhesion sites. however, the organization of these transmembrane components at invadopodia and adhesion sites differs. in addition, degradation of the ecm occurs at sites of the invadopodia, but not at focal adhesio ... | 1990 | 1965957 |
| analysis of b complex polymorphism in rous sarcoma progressor and regressor chickens with b-g, b-f, and b-l beta probes. | molecular polymorphism of the b complex was studied in serologically defined b19 haplotypes by use of class i, class ii, and class iv probes in southern blot experiments in chickens. all chickens studied shared identical class iv restriction patterns. in contrast, class i and class ii probes revealed six and five subtypes of b19 haplotype, respectively. these subtypes may be resolved in three homozygous genotypes and their corresponding heterozygous combinations. previous genetic selection allow ... | 1990 | 1980812 |
| interleukin-1-inhibitor activity induced by respiratory syncytial virus: abrogation of virus-specific and alternate human lymphocyte proliferative responses. | respiratory syncytial virus (rsv) infection has been shown to induce human mononuclear leukocyte (mnl) production of net interleukin-1 (il-1)-inhibitor activity. in the current studies of il-1-inhibitor effects, rsv-exposed cells were compared with autologous mnl that were sham-exposed or exposed to inactivated rsv or influenza virus (which induces net il-1 activity and commonly elicits effective homotypic immunity). exposure of mnl to influenza virus or inactivated rsv resulted in increased exp ... | 1991 | 1984478 |
| effectiveness of enteric immunization in the development of secretory immunoglobulin a response and the outcome of infection with respiratory syncytial virus. | cotton rats were immunized via intranasal, intradermal, or enteric routes with respiratory syncytial virus (rsv) or a live recombinant vaccinia virus expressing the rsv f glycoprotein (vaccinia f). the animals were tested for the appearance of rsv-specific antibody responses in the serum, bronchoalveolar lavage, and nasal wash after immunization and for virus replication 4 days after intranasal challenge with rsv. rsv antibody response in the serum and respiratory tract was demonstrated in all i ... | 1991 | 1987374 |
| comparison of the virulence of wild-type thymidine kinase (tk)-deficient and tk+ phenotypes of vaccinia virus recombinants after intranasal inoculation of mice. | a recombinant vaccinia virus vector was constructed which expressed the major surface glycoprotein g of human respiratory syncytial virus (rsv) and the thymidine kinase (tk) gene of vaccinia virus. the virulence of this tk+ recombinant virus was compared with that of a tk- recombinant and the wild-type (wt) virus after intranasal inoculation of mice. respiratory infection with wt virus resulted in a lethal infection with widespread dissemination of virus. in contrast, infection with the tk- reco ... | 1991 | 1990060 |
| nosocomial transmission of respiratory syncytial virus in immunocompromised adults. | respiratory syncytial virus (rsv) isolates obtained from nine infected immunocompromised adult patients hospitalized during two consecutive winters (january through april 1987 and 1988) were collected and analyzed against a panel of monoclonal antibodies by an enzyme immunoassay. the history of the patients' illness, onset of symptoms, and date of initial isolation of virus was correlated with the hospital ward and time of hospitalization. three patients died of respiratory failure related to rs ... | 1991 | 1993745 |
| ambisense segment 3 of rice stripe virus: the first instance of a virus containing two ambisense segments. | the complete nucleotide sequence of rice stripe virus (rsv) segment 3 shows that it has two open reading frames, one in the viral-complementary sequence, which codes for the nucleocapsid protein, and the other in the viral-sense sequence. the non-coding region between the ambisense genes in rsv segment 3 contains several u and a tracts, as do the ambisense s segments of phleboviruses and uukuviruses. as we have previously shown that rsv segment 4 has an ambisense nature, this is the first instan ... | 1991 | 1993885 |
| pattern of respiratory syncytial virus epidemics in finland: two-year cycles with alternating prevalence of groups a and b. | time-resolved fluoroimmunoassay with monoclonal antibodies distinguishing between respiratory syncytial virus (rsv) group a and b strains was used to analyze their prevalence in finland during 1981-1990 among 3285 patients with laboratory diagnosis of rsv, most of them hospitalized. the group typing of antigens in 608 rsv-positive nasopharyngeal aspirates showed a regular alternation of group prevalence, following the cyclic occurrence of the virus. group a predominated in 73%-90% of specimens f ... | 1991 | 1995719 |
| vaccination of cotton rats with a chimeric fg glycoprotein of human respiratory syncytial virus induces minimal pulmonary pathology on challenge. | the cotton rat model of human respiratory syncytial virus (rsv) infection was used to study the safety and efficacy of a chimeric fg glycoprotein that was expressed in insect cells using a baculovirus vector. histologic and virologic examination of vaccinated rat lungs was done after challenge with rsv. when rats were challenged 1 month after vaccination, severe pulmonary inflammation characterized by both a mononuclear and polymorphonuclear cell infiltrate and 30%-40% involvement of lung tissue ... | 1991 | 1995720 |
| respiratory syncytial virus (rsv) f, g, m2 (22k), and n proteins each induce resistance to rsv challenge, but resistance induced by m2 and n proteins is relatively short-lived. | the ability of recombinant vaccinia viruses that separately encoded 9 of the 10 known respiratory syncytial virus (rsv) proteins to induce resistance to rsv challenge was studied in balb/c mice. resistance was examined at two intervals following vaccination to examine early (day 9) as well as late (day 28) immunity. balb/c mice were inoculated simultaneously by the intranasal and intraperitoneal routes with a recombinant vaccinia virus encoding one of the following rsv proteins: f, g, n, p, sh, ... | 1991 | 1995956 |
| respiratory syncytial virus infection of human mononuclear phagocytes stimulates synthesis of platelet-activating factor. | production of platelet-activating factor 1-o-alkyl-2-acetyl-sn-glycero-3- phosphocholine (paf), a potent mediator of inflammation, by mononuclear phagocytes varies with their stage of cellular differentiation and the nature of the eliciting stimulus. the human monocytic cell line u937 can be induced to differentiate to a macrophage-like cell following phorbol myristate acetate exposure, and after differentiation, these cells efficiently support replication of respiratory syncytial virus (rsv). u ... | 1991 | 2005091 |
| [virologic diagnosis of interstitial pneumonia]. | viral pneumonia is not very frequent in healthy adults; it is mostly observed in elderly subjects infected with an influenza virus, a respiratory syncytial virus (rsv) or an adenovirus (adv). in immunocompromised subjects, cytomegalovirus (cmv) is the most common of causal agents. from the point of view of clinicians, virological diagnostic methods must be rapid and specific, and for this reason direct examination techniques for respiratory specimens and immunological detection of virus are curr ... | 1991 | 2008581 |
| dual-enzyme cascade-magnetic separation immunoassay for respiratory syncytial virus. | a new immunoassay developed for the detection of respiratory syncytial virus (rsv) makes use of magnetic separation and amplification by a dual-enzyme cascade for signal generation. magnetic particles are conjugated to monoclonal anti-rsv antibodies through the heterobifunctional crosslinker sulfosuccinimidyl 4-(maleimidomethyl)cyclohexane-1-carboxylate, yielding particles of high specific activity and low background. the dual-enzyme cascade is initiated by activation of a masked inhibitor for t ... | 1991 | 2010616 |
| multicenter study of strains of respiratory syncytial virus. | two major groups of respiratory syncytial virus (rsv) strains, a and b, have been identified and their patterns of isolation determined in different communities but not simultaneously in multiple communities. in this study, we tested 483 rsv isolates from 14 university laboratories in the united states and canada for the 1984/1985 and 1985/1986 rsv seasons; 303 (63%) isolates were group a, 114 (24%) were group b, and 66 (14%) could not be grouped. isolates were subdivided into six subgroups with ... | 1991 | 2010623 |
| immunity to and frequency of reinfection with respiratory syncytial virus. | to better understand the duration of immunity against respiratory syncytial virus (rsv) and the role of serum antibodies to the surface glycoproteins, f and g, in susceptibility to reinfection, 15 adults with previous natural rsv infection were challenged with rsv of the same strain group (a) at 2, 4, 8, 14, 20, and 26 months after natural infection. by 2 months about one-half and by 8 months two-thirds of the subjects became reinfected. each challenge resulted in infection in at least one-fourt ... | 1991 | 2010624 |
| antigenic and genomic diversity within group a respiratory syncytial virus. | antigenic analysis using monoclonal antibodies and genomic analysis using ribonuclease protection was done on 47 isolates of group a respiratory syncytial virus (rsv) recovered from children in st. louis during four rsv seasons. antigenic analysis identified four subgroups; of the three that included more than one member, those designated a/2 and a/2v had characteristic ribonuclease protection patterns. a third subgroup, a/4, exhibited more extensive genomic heterogeneity, but all isolates were ... | 1991 | 2010638 |
| respiratory syncytial virus infections on an adult medical ward. | eleven cases of respiratory syncytial virus (rsv) infection occurred in acutely ill hospitalized adults over a 7-week period. nosocomial illness was suspected in two patients. because rsv can cause serious infections in immunocompromised adults with the potential for nosocomial spread, the following recommendations are indicated: (1) during the winter months, early recognition and diagnosis of rsv infections both in hospital staff and in patients should be encouraged; (2) infected hospital perso ... | 1991 | 2012452 |
| complete nucleotide sequence of rna 3 of rice stripe virus: an ambisense coding strategy. | the rna 3 of rice stripe virus (rsv) isolate t was cloned and its nucleotide sequence was determined. the complete primary structure was found to consist of 2504 nucleotides. one putative open reading frame (orf), between nucleotides 2412 and 1444 in the 5'-proximal region of the virus complementary-sense rna, encoded a 322 amino acid protein with an mr of 35,134 that was identified as the coat protein. the other orf, between nucleotides 66 and 701 in the 5'-proximal region of the virus-sense rn ... | 1991 | 2016591 |
| management of the infant with respiratory syncytial virus. | this article examines updated clinical information concerning respiratory syncytial virus (rsv) infection including epidemiology, pathology, clinical manifestations, diagnosis, treatment, nosocomial infection, and prognosis. also presented is current information on ribavirin therapy, its side effects, and precautions. research related to the most effective isolation methodology is discussed, as well as nursing diagnoses based on gordon's functional health patterns and interventions for the infan ... | 1991 | 2019971 |
| human and bovine respiratory syncytial virus: immunopathologic mechanisms. | human respiratory syncytial virus (hrsv) is the major respiratory tract pathogen of infants and young children. bovine respiratory syncytial virus (brsv) is recognised as an important cause of respiratory tract disease in calves. both of these viruses and their respective diseases share many similarities. immunopathologic mechanisms have been proposed to be involved in the pathogenesis of respiratory syncytial virus (rsv) infections. this review examines the current understanding of the role of ... | 1991 | 2021054 |
| cytotoxic t cell activity against the 22-kda protein of human respiratory syncytial virus (rsv) is associated with a significant reduction in pulmonary rsv replication. | recombinant vaccinia viruses expressing the rsv f glycoprotein (vac-f), or a previously described chimeric protein consisting of the extracellular domains of the f and g glycoproteins (vac-fg), or the 22-kda membrane protein (vac-22 kda) were evaluated for their ability to protect balb/c mice against infection by rsv subgroup a or subgroup b viruses and for their ability to induce a humoral immune response or a cytolytic t lymphocyte (ctl) response. immunization with vac-f or vac-fg fully protec ... | 1991 | 2024493 |
| risk factors for respiratory syncytial virus-associated lower respiratory illnesses in the first year of life. | the relation of breast feeding and other factors to the incidence of respiratory syncytial virus-associated lower respiratory tract illness (rsv-lri) in the first year of life is examined. the study population is 1,179 healthy infants enrolled at birth between may 1980 and january 1984 into the tucson children's respiratory study, tucson, arizona. each subject's data were assessed at each month of age during the first year of life, during those months when respiratory syncytial virus was isolate ... | 1991 | 2035517 |
| detection of respiratory syncytial virus in nasopharyngeal secretions by shell vial technique. | a shell vial technique was used to recover respiratory syncytial virus (rsv) from frozen nasopharyngeal specimens previously tested by rapid diagnostic methods. with specimens determined to be positive by direct fluorescence assay (dfa), the shell vial technique was at least as sensitive as conventional tissue culture (92 versus 90%). the majority of rsv isolates were detected within 16 h postinoculation, versus an average of 4.5 days by conventional techniques. also, the shell vial method recov ... | 1991 | 2037662 |
| comparison of enzyme-linked immunosorbent assay, indirect immunofluorescence assay, and virus isolation for detection of respiratory viruses in nasopharyngeal secretions. | nasopharyngeal secretions obtained from 94 children with acute respiratory illness were examined for the presence of respiratory syncytial virus (rsv), adenovirus, and influenza virus type a by virus culturing (virus isolation technique [vit]), immunofluorescence assay (ifa), and enzyme-linked immunosorbent assay (elisa). similar results were obtained in at least two tests for rsv, influenza virus type a, and adenovirus in 92 (97.9%), 88 (93.6%), and 88 (93.6%) cases, respectively. both rapid vi ... | 1991 | 2037663 |
| comparison of two rapid methods for detection of respiratory syncytial virus (rsv) (testpack rsv and ortho rsv elisa) with direct immunofluorescence and virus isolation for the diagnosis of pediatric rsv infection. | the ability of two commercial immunoassays to detect respiratory syncytial virus (rsv) in respiratory specimens was evaluated as follows: 152 specimens were tested by testpack rsv (abbott), and 72 were tested by ortho rsv elisa (ortho). test outcomes were compared with those of virus isolation alone, direct immunofluorescence assay (dfa) alone, or virus isolation and/or dfa. testpack rsv versus virus isolation showed 91% sensitivity, 96% specificity, 93% positive predictive value (ppv), and 95% ... | 1991 | 2037684 |
| effect of passive antibody on the immune response of cotton rats to purified f and g glycoproteins of respiratory syncytial virus (rsv). | the effect of passively transferred rsv immune serum on the antibody response to a single dose of purified rsv fusion (f) and large (g) glycoproteins was studied in cotton rats. passively transferred antibody that achieved serum antibody levels similar to those seen in newborn human infants resulted in a seven- to eightfold suppression of the neutralizing antibody response of cotton rats to low doses of purified f and g glycoproteins (0.2-1.7 micrograms) and a twofold suppression to higher doses ... | 1991 | 2042390 |
| a comparison of transcriptional regulatory element activities in transformed and non-transformed rat anterior pituitary cells. | transformed (gh-3) and non-transformed (p3) rat anterior pituitary cells were compared in their ability to direct expression of plasmids containing a variety of eukaryotic transcriptional regulatory elements (tres). these include the herpes simplex virus thymidine kinase (hsv-tk), rous sarcoma virus long terminal repeat (rsv-ltr), simian virus 40 early (sv-40e), human cytomegalovirus immediate-early (cmv-ie) and mouse metallothionein 1 (mmt-1) tres. chloramphenicol acetyl transferase (cat) gene ... | 1991 | 2050277 |
| sequence analysis of the polymerase l gene of human respiratory syncytial virus and predicted phylogeny of nonsegmented negative-strand viruses. | the complete nucleotide sequence of the large (l) polymerase gene of human respiratory syncytial virus (rsv) strain a2 was determined by analysis of cloned-cdnas representing the entire gene and confirmed in part by dideoxy sequencing of genomic rna. the rsv l gene is 6578 nucleotides in length and contains a single major open reading frame that encodes a protein of 2165 amino acids. the molecular weight (250,226) and amino acid composition of the deduced rsv l protein are similar to those of ot ... | 1991 | 2053282 |
| rapid identification of respiratory syncytial virus infections by direct fluorescent antibody testing: reliability as a guide to patient cohorting. | this study compared the results of a commercially available, direct fluorescent antibody (dfa) test with viral culture in 880 specimens obtained from 690 patients by means of nasopharyngeal swabs. the two tests were congruent in 92.5% (814) of the specimens. the sensitivity of the dfa was 0.95, the specificity was 0.91, the positive predictive value was 0.82, and the negative predictive value was 0.98. among 548 inpatients, there were 3 mixed infections (rsv and another virus), 8 rsv infections ... | 1991 | 2053715 |
| group-specific serum antibody responses in children with primary and recurrent respiratory syncytial virus infections. | antigenic group-specific serum antibody responses to first and second respiratory syncytial virus (rsv) infections were studied in children who had been followed longitudinally from early infancy in a research day-care center. plaque-reduction neutralizing (prn) antibody assays and elisas for the fusion (f) and attachment (g) glycoproteins were done using antigens of prototype rsv strains from groups a and b. responses to antigens of viruses homologous and heterologous to the antigenic group of ... | 1991 | 2056202 |
| evaluation of an enzyme membrane immunoassay (directigen rsv) for the rapid diagnosis of respiratory syncytial virus infections. | a rapid enzyme immunoassay (eia) membrane test, the directigen respiratory syncytial virus (becton dickinson), was compared with cell culture, an indirect immunofluorescence (if) test, the monofluokit respiratory syncytial virus (diagnostics pasteur), and a conventional enzyme immunoassay antigen test, the abbott respiratory syncytial virus enzyme immunoassay in nasal aspirates specimens from children with suspected respiratory syncytial virus (rsv) bronchiolitis. the sensibility and specificity ... | 1991 | 2062553 |
| early use of indirect immunofluorescence for the detection of respiratory syncytial virus in hep-2 cell culture. | respiratory syncytial virus is detected in cell culture by the presence of cytopathic effect. to detect rsv before cytopathic effect is usually seen, slides were evaluated retrospectively from 482 hep-2 cell cultures on days 2-4 after inoculation. indirect immunofluorescent staining detected rsv in 57 of 94 cultures that eventually were found positive by cytopathic effect. in an additional 19 cases that ultimately showed no cytopathic effect, rsv also was detected. in 15 of the latter cases, the ... | 1991 | 2069130 |
| respiratory syncytial virus infection in c57bl/6 mice: clearance of virus from the lungs with virus-specific cytotoxic t cells. | we describe respiratory syncytial virus (rsv)-specific cytotoxic t-cell (ctl) lines and clones developed from the spleens of c57bl/6 and balb/c mice. line 7 and clones derived from it were h-2kb restricted, whereas line 12 had both kb and db components. both lines, and all the clones except one, could lyse targets infected with either strain a or strain b rsv. line 7 or 7-11e1 cells (8 x 10(6) to 10 x 10(6) given intravenously cleared rsv from the lungs of infected mice. there was no morbidity o ... | 1991 | 2072460 |
| [ultrastructural changes in middle ear and cochlea caused by respiratory syncytial virus in guinea pigs]. | respiratory syncytial virus (rsv) was inoculated into the middle ear of guinea pigs. preyer's reflex began to decrease since 21st day. the positive rate of rsv culture from middle ear mucosa within the first week was 50%. the titer of igg antibody against rsv in sera rose gradually. electron microscopy showed enlarged rough endoplasmic reticulum, swelling mitochondria and pyknosis of nuclei in the epithelial and mononuclear cells of middle ear. the surface of corti's organ was covered with exuda ... | 1990 | 2076325 |
| primary pulmonary murine cytotoxic t lymphocyte specificity in respiratory syncytial virus pneumonia. | effector cells capable of lysing respiratory syncytial virus (rsv)-infected cells were isolated from the lungs of intranasally infected mice. an examination of their specificity showed that cytolysis was major histocompatibility complex restricted. using recombinant vaccinia viruses containing cloned rsv genes to infect target cells, balb/c (h-2d) pulmonary effector cells were shown to recognize the fusion protein (f) and to a lesser extent the nucleoprotein (n). cells specific for the major gly ... | 1990 | 2077342 |
| [seroepidemiologic studies of the distribution of respiratory syncytial virus in wild ruminants]. | sera from wild ruminants (302 roe, 39 red deer, 17 fallow deer, 1 mouflon) were tested for antibodies against respiratory syncytial virus (rsv). 22 sera (6.1%) were positive: 20 roe, 1 red deer, 1 mouflon. the findings are discussed from the epizootiological point of view. | 1990 | 2078187 |
| [the role of respiratory tract viruses in the etiology of obstructive bronchitis in infants]. | out of 524 children with acute respiratory infections in 141 obstructive bronchitis was diagnosed (ozo). seventy cases could be linked to viral infection. viral infections tested (influenza virus a, b, parainfluenza typ 1-3, rsv, adenoviruses) were more frequently associated with ozo than other acute respiratory infections of unknown etiology. majority infections induced by influenza virus a and parainfluenza virus typ 2 were accompanied by ozo symptoms. of the highest risk of acquiring ozo desp ... | 1990 | 2084449 |
| comparison of lung histopathology and bronchoalveolar lavage cytology in mice and cotton rats infected with respiratory syncytial virus. | lung histology as well as cell number and size distribution in bronchoalveolar lavage (bal) were compared in balb/c mice and in cotton rats, of various immune status regarding the respiratory syncytial virus (rsv), when subjected to challenge with rsv. in mock-immunized animals, rsv infection typically caused microscopic inflammatory lesions of the lungs and the presence of inflammatory cells in the bal. immunization with a formalin inactivated vaccine prior to challenge increased the severity o ... | 1990 | 2087875 |
| [evaluation of methods for the detection of syncytial respiratory virus in nasopharyngeal secretions]. | the screening for respiratory syncytial virus (rsv) in nasopharyngeal secretions with enzyme immunoassay (elisa) and indirect immunofluorescence (iif) has been evaluated in infants and young children with acute respiratory infection. both methods were compared with viral isolation in hep-2 cells and the investigation of fluorescent foci in cell cultures inoculated by centrifugation. 226 samples were evaluated by iff, 182 of which were also evaluated by elisa while 158 were inoculated into cell c ... | 1990 | 2098144 |
| [pneumonia in adults caused by the respiratory syncytial virus]. | respiratory syncytial virus (rsv) usually affects children but sometimes affects a certain group of adults. a case of an 81 year old female with idiopathic thrombocytopenic purpura who had pneumonia caused by rsv is presented. the literature is reviewed as well as the most frequent clinical manifestations, diagnosis methods and treatment. | 1990 | 2103289 |
| targeted immunoglobulin therapy for the prevention of neonatal infections. | premature infants demonstrate hypoglobulinemia and are at increased risk for serious infections. although a cause-and-effect relation between low serum igg levels and neonatal infections has not been established, prophylaxis of such severe infections may be possible by replacement of antibody with intravenous immunoglobulin (ivig). for success, ivig must provide specific antibodies to neonatal pathogens and reach therapeutic serum igg target levels. pilot investigations have demonstrated that iv ... | 1990 | 2114035 |
| acute and long-term effects of viral bronchiolitis in infancy. | about 1% of infants are admitted to hospital with acute bronchiolitis; 85% of cases are caused by infection with respiratory syncytial virus (rsv). the pathophysiological changes during the acute illness are inflammatory obstruction in the small airways with submucosal cellular infiltration, epithelial necrosis and mucous plugging; frc increases and dynamic compliance falls. failure to respond to bronchodilator drugs suggests that muscle spasm contributes relatively little to the airway narrowin ... | 1990 | 2117143 |
| economic and long-term benefits of ribavirin therapy on respiratory syncytial virus infection. | ribavirin is a broad-spectrum antiviral agent. administered as an aerosol, it has been shown to be clinically effective in improving the signs and symptoms of viral bronchiolitis in infancy, particularly cases due to respiratory syncytial virus (rsv). this paper reviews the evidence for economic and/or long-term clinical benefits from using ribavirin in the acute illness. there are data to suggest that use of ribavirin may lead to a reduction in therapeutic interventions and duration of hospital ... | 1990 | 2117144 |
| ig/ebp-1: a ubiquitously expressed immunoglobulin enhancer binding protein that is similar to c/ebp and heterodimerizes with c/ebp. | we report the isolation and characterization of cdna clones that encode a protein with the same dna binding specificity as the immunoglobulin heavy chain enhancer binding protein e (muebp-e). we call the gene encoding this protein ig/ebp-1. a fusion protein encoded by the cdna binds specifically to muebp-e-binding sites (e sites) in both the igh enhancer and the vh1 promoter. sequence analysis reveals that ig/ebp-1 is a member of the "basic-zipper" family of dna-binding proteins that are charact ... | 1990 | 2121606 |
| overexpression of avian or mouse c-jun in primary chick embryo fibroblasts confers a partially transformed phenotype. | the coding sequences of avian (quail) or murine c-jun proto-oncogenes were introduced into a non-defective retroviral vector derived from rous sarcoma virus (rsv) in which c-jun replaces v-src. primary avian fibroblasts chronically infected with either one of these viruses exhibit some phenotypic traits characteristic of rsv-transformed cells, including sustained growth in low serum medium and ability to develop colonies from single cells in agar, even though they are still of normal morphology ... | 1990 | 2123532 |
| a study of nonbacterial agents of acute lower respiratory tract infection in thai children. | from january 1986 to december 1987, 596 children less than 5 years of age with lower respiratory tract infection (lri)--manifested as laryngitis, croup, bronchitis, bronchiolitis, and pneumonia--were studied for evidence of infection with respiratory tract viruses mycoplasma pneumoniae, and chlamydia trachomatis. of the 596 children in the study, 315 were ambulatory and 281 were hospitalized. virologic studies included isolation and rapid diagnosis of virus from specimens of nasopharyngeal aspir ... | 1990 | 2125359 |
| respiratory syncytial virus modulation of adult and neonatal lymphocyte mitogenic responses and the role of interferon-gamma. | infection of human adult and neonatal mononuclear leukocytes (mnls) with respiratory syncytial virus (rsv) caused significant inhibition of lymphoproliferation in response to various mitogens (heat-killed staphylococcus aureus, phytohaemagglutinin, concanavalin-a and pokeweed mitogen). antibody to human interferon-gamma enhanced the rsv-induced suppression of lymphoproliferation in adult, but not neonatal mnls. these results are consistent with the hypothesis that rsv can suppress the developmen ... | 1990 | 2129048 |
| lymphocyte subpopulations in peripheral blood of lambs experimentally infected with bovine respiratory syncytial virus. | the lymphocyte subpopulations of peripheral blood of normal lambs and lambs experimentally infected with bovine respiratory syncytial virus (rsv) were analysed by flow cytometry, using a panel of monoclonal antibodies against specific lymphocyte epitopes. experimental infection with bovine rsv was characterized by a significant rise in sbu-t8+ (cd8+ or cytotoxic) t cells and a significant reduction in sbu-t4+ (cd4+ or helper) t cells and b (lca p220+) lymphocytes (p less than 0.05). the helper/s ... | 1990 | 2140217 |
| coding strategy of rice stripe virus: major nonstructural protein is encoded in viral rna segment 4 and coat protein in rna complementary to segment 3. | the two major proteins found in plants infected with rice stripe virus (rsv), coat protein and a major nonstructural protein (major ns), were purified and their partial amino acid sequences were determined. oligonucleotides were synthesized according to the amino acid sequence information, and used as probes for northern blot analyses of four single-stranded rna species (segments 1-4) and four double-stranded rna species of rsv. the results indicated that the coding strategy of rsv was similar t ... | 1990 | 2141205 |
| protection from herpes simplex virus type 1 lethal and latent infections by secreted recombinant glycoprotein b constitutively expressed in human cells with a bk virus episomal vector. | the herpes simplex virus type 1 (hsv-1) glycoprotein b (gb-1) gene, deleted of 639 nucleotides that encode the transmembrane anchor sequence and reconstructed with the extramembrane and intracytoplasmic domains, was cloned under control of the rous sarcoma virus long terminal repeat in the episomal replicating vector prp-rsv, which contains the origin of replication and early region of the human papovavirus bk as well as a cdna for a mutant mouse dihydrofolate reductase that is resistant to meth ... | 1990 | 2152829 |
| identification of a third protein factor which binds to the rous sarcoma virus ltr enhancer: possible homology with the serum response factor. | we have identified a new protein factor (efiii) in nuclear extracts of quail fibroblasts and chick embryos which binds specifically in vitro to a 26-bp region of the rous sarcoma virus (rsv) long terminal repeat (ltr) enhancer. the efiii binding site in the rsv ltr exhibits a strong sequence homology to the serum response element (sre). the sre is a 22-bp cis-acting dna sequence element, first identified upstream of the human c-fos gene, which can confer serum inducibility to heterologous promot ... | 1990 | 2152992 |
| plasminogen activator gene expression is induced by the src oncogene product and tumor promoters. | secretion of urokinase-type plasminogen activator (upa) by chicken embryo fibroblasts (cef) is increased approximately 50-fold following transformation by rous sarcoma virus (rsv). using a cloned and fully sequenced chicken upa cdna probe, we have established that this increase in plasminogen activator production can be largely accounted for by an increase in cellular upa mrna. cef contained on average less than 1 molecule of upa mrna/cell, whereas rsv-cef contained 25-60 molecules/cell. the inc ... | 1990 | 2153128 |
| localization of 9e3/cef-4 in avian tissues: expression is absent in rous sarcoma virus-induced tumors but is stimulated by injury. | the avian gene 9e3/cef-4, a member of the superfamily of genes that includes kc and gro, is expressed abundantly in exponentially growing cultures of chick embryo fibroblasts (cefs) and at high levels in cefs transformed with rous sarcoma virus (rsv). the product of this gene is a secreted protein that has homologies and structural similarities to inflammatory mediators. the function of 9e3 is obscure and its expression in vivo has not yet been investigated. we studied by in situ hybridization a ... | 1990 | 2155240 |
| a study of the dimer formation of rous sarcoma virus rna and of its effect on viral protein synthesis in vitro. | the genetic material of all retroviruses examined so far is an rna dimer where two identical rna subunits are joined at their 5' ends by a structure named dimer linkage structure (dls). since the precise location and structure of the dls as well as the mechanism and role(s) of rna dimerization remain unclear, we analysed the dimerization process of rous sarcoma virus (rsv) rna. for this purpose we set up an in vitro model for rsv rna dimerization. using this model rsv rna was shown to form dimer ... | 1990 | 2155394 |
| glycyl-l-glutamine stimulates the accumulation of a12 acetylcholinesterase but not of nicotinic acetylcholine receptors in quail embryonic myotubes by a cyclic amp-independent mechanism. | myotubes prepared from the japanese quail embryo at 9 days gestation were cultivated in the presence of glycyl-l-glutamine (gly-gln, beta-endorphin c-terminal dipeptide) or glycyl-glutamic acid (gly-glu), and changes in the activity of acetylcholinesterase (ache) molecular forms and binding of 125i-alpha-bungarotoxin (alpha bgt) to cell surface nicotinic acetylcholine receptors were measured. the a12 oligomer was the major form of ache in the cultures. the activity of all molecular forms of the ... | 1990 | 2156012 |
| surveillance of viral respiratory tract infections over a one year period in mainly hospitalized austrian infants and children by a rapid enzyme-linked immunosorbent assay diagnosis. | in order to obtain more information on viral respiratory tract infections in austrian infants and children, nasopharyngeal secretions from 1432 infants and children, collected from october 1984 to october 1985, were screened for the presence of respiratory syncytial virus (rsv), adenoviruses, parainfluenza virus type 1, 2, and 3, and influenza viruses type a and b, by enzyme-linked immunosorbent assay (elisa). the results obtained were analyzed with respect to incidence, seasonal distribution an ... | 1990 | 2156381 |
| evaluation of homology modeling of hiv protease. | the model of human immunodeficiency virus (hiv-1) protease which was based on the crystal structure of rous sarcoma virus (rsv) protease has been compared to the recently determined crystal structure of chemically synthesized hiv-1 protease. the overall difference between the model and crystal structure was 1.4 a root mean square (rms) deviation for 86 superimposed c alpha atoms. the position of the flexible flap differs in the model and six residues at the amino terminus were incorrectly placed ... | 1990 | 2158092 |
| serum and v-src increase the level of a ccaat-binding factor required for transcription from a retroviral long terminal repeat. | transcription from the long terminal repeat (ltr) of rous sarcoma virus (rsv) in rat 3y1 fibroblasts was dependent on the presence of serum. within 1 hr after addition of serum to a serum-deprived culture, there was a fivefold increase in the level of transcripts initiated at the ltr. this stimulation did not require synthesis of new proteins. the induction of transcription by serum was mostly dependent on two ccaat boxes in the ltr. within 1 hr after addition of serum, there was also an increas ... | 1990 | 2159932 |
| localization by mutational analysis of transcription factor binding sequences in the u3 region of rous sarcoma virus ltr. | the transcription factor binding sequences in the u3 region of rous sarcoma virus ltr have been determined by gel retardation assays using mutant synthetic oligonucleotides. the results indicate that the factor, e2bp, specifically binds to sequences tgcaatac and tgcaacat, which are localized between nucleotides -222 to -215 and -203 to -196, respectively. this factor is present at elevated levels in avian qt6 cells compared to mouse 3t3 and rat 2 tk- cells. e2bp binds to a sequence that is simil ... | 1990 | 2161147 |
| distinct and different effects of the oncogenes v-myc and v-src on avian sympathetic neurons: retroviral transfer of v-myc stimulates neuronal proliferation whereas v-src transfer enhances neuronal differentiation. | immature avian sympathetic neurons are able to proliferate in culture for a limited number of divisions albeit expressing several neuron-specific properties. the effect of avian retroviral transfer of oncogenes on proliferation and differentiation of sympathetic neurons was investigated. primary cultures of 6-d-old quail sympathetic ganglia, consisting of 90% neuronal cells, were infected by myelocytomatosis virus (mc29), which contains the oncogene v-myc, and by the v-src-containing rous sarcom ... | 1990 | 2161856 |
| transcriptional activity of the rous sarcoma virus long terminal repeat correlates with binding of a factor to an upstream ccaat box in vitro. | the avian nuclear protein, enhancer factor 1 (ef1), binds specifically to the long terminal repeat (ltr) of rous sarcoma virus (rsv) in a region that has been implicated in enhancer/promoter function. we have characterized the in vitro binding properties of this factor from chick embryo nuclear extracts by methylation interference/protection foot-printing of the wild-type ltr and also by gel electrophoretic mobility shift assays performed on a series of ltr mutants. we find that the inverted cca ... | 1990 | 2162108 |
| [molecular basis of retrovirus adaptation: nucleotide sequence of rous sarcoma virus adapted to duck cells]. | the host range of retroviruses is rather complex and specific. it is controlled by the products of viral structural genes that interact with the determinants both on the surface and within the cell. the possibility to infect and transform duck embryo fibroblasts is shown for the prague strain of chicken rous sarcoma virus (subgroup c), though virus production in these cells is restricted. however, after the 6th passage the "adapted" virus gave the titre practically the same as it was for chicken ... | 1990 | 2162309 |
| comparison of the crystal structures and intersubunit interactions of human immunodeficiency and rous sarcoma virus proteases. | the crystal structures of the proteases (prs) encoded by the rous sarcoma virus (rsv) and the human immunodeficiency virus (hiv) have been compared. the crystallographic monomer of hiv pr superimposes on the two crystallographically independent subunits of the rsv pr dimer with root mean square deviations of 1.45 and 1.55 a for 86 and 88 common c alpha atoms, respectively. there is a conserved structural core consisting of seven beta-strands forming two perpendicular layers, a helix, and the ami ... | 1990 | 2162350 |
| down regulation by p60v-src of genes specifically expressed and developmentally regulated in postmitotic quail neuroretina cells. | the avian neuroretina (nr) is composed of photoreceptors and different neurons that are derived from proliferating precursor cells. neuronal differentiation takes place after terminal mitosis. we have previously shown that differentiating nr cells can be induced to proliferate by infection with rous sarcoma virus (rsv) and that cell multiplication requires expression of a functional v-src gene. we speculated that the quiescence of nr cells could be determined by specific genes. cell proliferatio ... | 1990 | 2162475 |
| mediation of wound-related rous sarcoma virus tumorigenesis by tgf-beta. | in rous sarcoma virus (rsv)-infected chickens, wounding leads to tumor formation with nearly 100% frequency in tissues that would otherwise remain tumor-free. identifying molecular mediators of this phenomenon should yield important clues to the mechanisms involved in rsv tumorigenesis. immunohistochemical staining showed that tgf-beta is present locally shortly after wounding, but not unwounded controls. in addition, subcutaneous administration of recombinant transforming growth factor-beta 1 ( ... | 1990 | 2163544 |
| potent growth inhibition of human tumor cells in culture by arginine deiminase purified from a culture medium of a mycoplasma-infected cell line. | two kinds of growth-inhibitory substances were found in culture of a rous sarcoma virus-transformed rat liver cell line, rsv-brl. the two substances were purified from the serum-free culture medium and identified as transforming growth factor beta 1 and mycoplasma-derived arginine deiminase (ec 3.5.3.6), respectively. the arginine deiminase was an acid-labile but dithiothreitol-resistant protein with a molecular weight of 45,000 and pi 4.7. its km value for l-arginine was 0.3 mm, which is about ... | 1990 | 2164440 |
| transformation-defective mutants with 5' deletions of the src gene are frequently generated during replication of rous sarcoma virus in established quail fibroblasts. | replication of rous sarcoma virus (rsv) in avian fibroblasts leads to the generation of replication-competent variants that are defective for cell transformation (td virus). these td variants contain deletions affecting various portions of the v-src gene. we compared the rate of td virus production in q3b cells, a quail cell line established by mutagen treatment, and in normal quail fibroblasts. twenty-five days after infection with an rsv stock containing only transforming virions, q3b cells ha ... | 1990 | 2164723 |
| a group level analysis of the associations between antibodies to seven putative pathogens and respiratory disease and weight gain in ontario feedlot calves. | the associations, at the group level, between serological titer to pasteurella haemolytica surface antigens (ph), pasteurella haemolytica cytotoxin (ph-cytox), infectious bovine rhinotracheitis virus (ibrv), bovine virus diarrhea virus (bvdv), parainfluenza-3 virus (piv3), respiratory syncytial virus (rsv), mycoplasma dispar (md), m. bovis (mb), and respiratory disease treatment rates, relapse rates, and 28 day weight gains were investigated in 14 groups of calves entering two feedlots during ye ... | 1990 | 2165846 |
| fusion of rous-sarcoma-virus-transformed rat cells to morphologically normal human or rat cells results in transcriptional suppression of the provirus that depends on its chromosomal integration site. | the fusion of a rous sarcoma virus (rsv)-transformed rat fibroblast clone to at least 2 different human cell types reproducibly produces phenotypically normal hybrids. analysis of such hybrids reveals that proviral silence is the result of transcriptional down-regulation, presumably by a trans-acting human molecule. furthermore, this phenomenon seems to be strongly influenced by the proviral chromosomal integration site and its imposition may entail a mechanism that is required only transiently. | 1990 | 2166711 |
| incorporation of chimeric gag protein into retroviral particles. | the product of the rous sarcoma virus (rsv) gag gene, pr76gag, is a polyprotein precursor which is cleaved by the viral protease to yield the major structural proteins of the virion during particle assembly in avian host cells. we have recently shown that myristylated forms of the rsv gag protein can induce particle formation with very high efficiency when expressed in mammalian cells (j. w. wills, r. c. craven, and j. a. achacoso, j. virol. 63:4331-4343, 1989). we made use of this mammalian sys ... | 1990 | 2166812 |
| pp60v-src association with the cytoskeleton induces actin reorganization without affecting polymerization status. | the mechanism by which rous sarcoma virus (rsv) induces a reorganization of actin and its associated proteins and a reduction in microfilament bundles is at present poorly understood. to examine the relationship between the organization of the microfilament system and the polymerization state of actin after transformation, we have investigated these changes in a rat-1 cell line transformed by la29, a temperature-sensitive (ts) mutant of rsv. parallel immunofluorescence and biochemical analysis d ... | 1990 | 2167224 |
| morphological aspects of inhibition of transformation of rat kidney cells infected with a temperature-sensitive mutant of the rous sarcoma virus by rat fibroblast interferon. | normal rat kidney cells (nrk) infected with a temperature-sensitive (ts) mutant of rous sarcoma virus (rsv) at the permissive temperature (33 degrees c) exhibit the morphological and growth characteristics of the transformed state. by the use of this system, it has been shown that the addition of rat fibroblast interferon to infected cells grown at a nonpermissive temperature (38 degrees c) prevents re-transformation when they are introduced to the permissive temperature. merely treating cells a ... | 1990 | 2168311 |
| the effects of 3-deazaguanine on rous sarcoma virus transformation. | an expression of cellular transformation in chicken embryo fibroblast infected with rous sarcoma virus (rsv) was inhibited by 3-deazaguanine (3-dg). re-transformation of normal rat kidney cells infected with a temperature-sensitive mutant of rsv was suppressed by 3-dg when the cells were shifted from the non-permissive temperature (38 degrees c) to the permissive temperature (33 degrees c). removal of the inhibitor enabled the cells to revert to the transformed state. finally, 3-dg had little or ... | 1990 | 2168314 |
| fusion of rous sarcoma virus with host cells does not require exposure to low ph. | we investigated whether rous sarcoma virus (rsv) infects cells through a ph-independent or a low-ph-dependent pathway. to do this, the effects of lysosomotropic agents and acid pretreatment on rsv infectivity of, and fusion with, chicken embryo fibroblasts (cefs) were studied. high concentrations of lysosomotropic agents (ammonium chloride and monensin) did not inhibit virus infectivity: equal titers of rsv were produced in the presence and absence of these agents. similarly, low-ph pretreatment ... | 1990 | 2168989 |
| [antiviral and interferon-inducing effect of fang-gan mixture]. | the present study was carried out by using the plaque formation test, microdosage cytopathic effect (cpe) method, and mice nasal-cavity-attacking method to observe the antiviral and interferon-inducing effect of fang-gan mixture (fgm). the results showed that fgm has direct respiratory syncytia virus (rsv) inactivation effect, and protection effect after mice infected by rsv. fgm could also decrease mice death rate (p less than 0.01) in the experimentation of using parainfluenza virus to attack ... | 1990 | 2169355 |
| malignant transformation of human endometrial stromal cells by transfection of c-myc: effects of prsvneo cotransfection and treatment with mnng. | stromal cells isolated from normal human endometrium were cotransfected in primary culture with psvc-myc, a plasmid containing a truncated c-myc gene regulated by simian virus 40 promoter, and prsv neo, a plasmid containing a neomycin resistance gene regulated by rous sarcoma virus (rsv) promoter. these cells demonstrated properties of transformed cells in vitro, including altered morphology, focus formation, anchorage-independent growth, chromosomal alterations, and tumor formation in athymic m ... | 1990 | 2171549 |
| transactivation of the rous sarcoma virus long terminal repeat promoter by marek's disease virus. | transient expression of chloramphenicol acetyltransferase (cat) was used to study marek's diseases virus (mdv)-mediated transactivation of the rous sarcoma virus long terminal repeat (rsv-ltr) promoter. cotransfection experiments in primary avian cells were conducted using mdv high-molecular-weight dna and plasmid prsvcat. increased cat activity, relative to controls, was consistently observed in the presence of mdv. enhanced cat activity, expressed via the rsv-ltr promoter, was strictly depende ... | 1990 | 2173259 |
| sequence specificity of mrna n6-adenosine methyltransferase. | the sequence specificity of chicken mrna n6-adenosine methyltransferase has been investigated in vivo. localization of six new n6-methyladenosine sites on rous sarcoma virus (rsv) virion rna has confirmed our extended consensus sequence for methylation: rgacu, where r is usually a g (7/12). we have also observed a (2/12) and u (3/12) at the -2 position (relative to m6a at +1) but never a c. at the +3 position, the u was observed 10/12 times; an a and a c were observed once each in weakly methyla ... | 1990 | 2173695 |
| a retroviral promoter is sufficient to convert proto-src to a transforming gene that is distinct from the src gene of rous sarcoma virus. | the src genes of four natural isolates of avian sarcoma viruses differ from cellular proto-src in two genetic substitutions: the promoter of the cellular gene is replaced by a retroviral counterpart, and at least six codons from the 3' terminus are replaced by retroviral or heterologous cell-derived elements. since virus constructs with a complete proto-src coding region failed to transform avian cells but acquired transforming function by point mutations of various codons, it has been proposed ... | 1990 | 2174554 |
| isolation and identification of viral agents in argentinian children with acute lower respiratory tract infection. | from a total population of 1,002 children with acute lower respiratory infection (alri), identification of virus was achieved in 304 cases. in this survey, 1,000 nasopharyngeal aspirate and 13 lung tissue samples were tested in four cell lines (hep-2, mrc-5, mdck, and llc-mk2) and by indirect immunofluorescence (iif), while 242 paired sera were studied by complement fixation. respiratory syncytial virus (rsv) was the most frequently detected agent (n = 183), followed by adenovirus (n = 28), para ... | 1990 | 2176736 |
| acute lower respiratory tract infection due to virus among hospitalized children in dhaka, bangladesh. | the nature of acute lower respiratory tract infection (alri) in hospitalized children and of the associated viral agents was assessed in a study of 601 children less than 5 years old over a 24-month period. of these children, 80% were less than 24 months of age and the ratio of boys to girls was 1.7:1. pneumonia (86.5% of cases) was the most frequently observed clinical manifestation. shedding of virus was detected in 21.1% of the children; the highest rate occurred in infants 0-5 months old (27 ... | 1990 | 2176737 |
| regulation of integrin mobility and cytoskeletal association in normal and rsv-transformed chick embryo fibroblasts. | the mobility of the integrin receptor in trypsinised chick embryo fibroblasts (cef) was investigated using the csat monoclonal antibody. the binding of csat to trypsinised cef followed by incubation at 37 degrees c resulted in patching and then capping of the receptor. this capping was dependent on cellular metabolism, since agents such as sodium azide or 2-deoxyglucose inhibited the process. whereas about 95% of unclustered integrin was soluble in nonidet p40-containing buffers, after capping m ... | 1990 | 2177479 |
| an epidemic of respiratory syncytial virus in elderly people: clinical and serological findings. | in 1984-1985, an outbreak of respiratory syncytial virus (rsv) infection occurred in two geriatric wards. among 68 patients (mean age +/- sd = 82.5 +/- 12.5 with respiratory signs, 52 had signs caused by rsv infection. among all patients, the clinical and serological attack rates were 61.2% and 75.0%, respectively. the most frequent clinical presentation was intensive coughing (96.1%) and fever (96.1%) associated with expectorate (63.5%). the duration of the respiratory symptoms was 5 to 7 days. ... | 1990 | 2179469 |
| [once more a discussion of the rsv affair]. | during autumn- and winter epidemics respiratory syncytial (rs) virus accounts for the majority of respiratory infections in infants and young children. in case of an acute lower respiratory tract infection, rs virus can induce serious symptoms. these are age-dependent. the most important symptoms in babies and toddlers are dyspnea, wheezing, cyanosis and apneas. in the case of respiratory insufficiency or fatigue, as well as recurrent apneas, mechanical ventilation is required. diagnosis can be ... | 1990 | 2180118 |
| comparison of directigen rsv with viral isolation and direct immunofluorescence for the identification of respiratory syncytial virus. | an enzyme immunoassay membrane test (directigen rsv) for the detection of respiratory syncytial virus in clinical specimens was compared prospectively with isolation in cell culture and direct immunofluorescence (if). a total of 315 nasopharyngeal wash specimens from pediatric patients were examined. directigen rsv was 86.1% sensitive and 91.3% specific for specimens positive by isolation in cell culture and/or if, with 88.6% agreement. the false-positive rate was 16%; 2 of 20 specimens giving f ... | 1990 | 2182668 |
| evaluation of five methods for respiratory syncytial virus detection. | a total of 117 nasal aspirates were cultured for respiratory syncytial virus (rsv) and tested for rsv antigen by a direct fluorescent-antibody (dfa) test (bartels immunodiagnostic supplies, inc., bellevue, wash.), the directigen enzyme immunoassay (eia; becton dickinson microbiology systems, cockeysville, md.), the testpack eia (abbott laboratories, north chicago, ill.), and rsv eia (abbott). agreement of two of five methods or a positive rsv culture were required to validate a result. a total o ... | 1990 | 2191003 |
| immunoprophylaxis of infections with respiratory syncytial virus: observations and hypothesis. | infections with respiratory syncytial virus (rsv) may cause substantial morbidity and increased mortality in neonates and infants, especially those born before term and/or with pulmonary or cardiac disease. humoral immunity was long thought to play only a small role in prevention, reduction of severity, or recovery from rsv infections of the lower respiratory tract. we recently performed a series of experiments in rsv-infected cotton rats, owl monkeys, and human infants to assess the role of hum ... | 1990 | 2194271 |
| a rapid test for detection of respiratory syncytial virus in nasopharyngeal secretion. | a new rapid membrane enzyme immunoassay (meia; directigen rsv) for detection of respiratory syncytial virus (rsv) was evaluated using samples of nasopharyngeal secretion from infants and children with acute respiratory disease. the meia was compared with an immunofluorescent antibody (if) technique using a sensitive biotin-avidin (ba) eia as reference. of 242 samples tested, 108 were positive by the meia and 123 by the ba-eia. of 144 samples which were also tested by the if technique, 57 were po ... | 1990 | 2197094 |
| what is the clinical role of aerosolized ribavirin? | aerosolized ribavirin is the first approved agent for the treatment of respiratory syncytial virus (rsv) infection. however, the clinical use of ribavirin is controversial. although ribavirin-treated patients appear to be subjectively improved, the benefit in terms of decreased morbidity and mortality is not well established. furthermore, the drug has been found to be teratogenic, carcinogenic, and mutagenic, depending upon the scientific model. because of its unique mode of administration, envi ... | 1990 | 2197815 |
| rapid detection of respiratory syncytial virus and influenza a virus in cell cultures by immunoperoxidase staining with monoclonal antibodies. | peroxidase-labeled monoclonal antibodies against respiratory syncytial virus (rsv) and influenza a virus were used for immunoperoxidase staining (ips) of cell cultures inoculated with nasopharyngeal aspirates. cells were grown in 24-well plates, and specimens were inoculated by low-speed centrifugation. cultures were incubated for 2 days at 37 degrees c and then fixed, stained, and observed by light microscopy. ips was compared with standard virus isolation by using cultures of human diploid fib ... | 1990 | 2199488 |
| detection of respiratory syncytial virus antigen in nasal washings by abbott testpack enzyme immunoassay. | we compared the new abbott testpack (tp) respiratory syncytial virus (rsv) enzyme immunoassay (eia) with cell culture and two commercial rsv eias (from abbott diagnostics and kallestad laboratories) by using split samples of fresh nasal washings from children with suspected rsv disease. two tubes of hep-2 cells were inoculated and observed for cytopathic effect for 14 days, and isolates were confirmed by immunofluorescence. the tp eia was performed by following the manufacturer's instructions. s ... | 1990 | 2199500 |
| detection of respiratory syncytial virus antigen after seventy-two hours of culture. | between september, 1987, and april, 1989, three techniques for the detection of respiratory syncytial virus (rsv) were compared: indirect immunofluorescence (if) on the sample, indirect immunofluorescence after 72 h of mrc-5 cell culture (if 72h), and detection of the cytopathic effect (cpe) by mrc-5 and hep-2 cell culture. a study of 383 nasal aspirates from young children admitted to the centre hospitalier de nantes (chr) showing miscellaneous respiratory symptoms produced the following result ... | 1990 | 2202784 |
| comparison of immunofluorescence & culture for the diagnosis of respiratory syncytial virus infection. | in 809 infants and children with acute respiratory infection, hep-2 cells were used for the isolation of respiratory syncytial virus (rsv) and an indirect immunofluorescence technique (iif) was used for the detection of rsv antigen in the epithelial cells of nasopharyngeal secretions. while rsv was detected in culture in only 87 subjects, iif was positive for viral antigen in 158 subjects. in children with bronchiolitis and in those with pneumonia 57 and 19 per cent respectively, had evidence of ... | 1990 | 2228050 |
| occurrence of groups a and b of respiratory syncytial virus over 15 years: associated epidemiologic and clinical characteristics in hospitalized and ambulatory children. | over 15 years respiratory syncytial virus (rsv) isolates from 1209 hospitalized and ambulatory children were examined for strain group and in a subset for subgroup to determine the associated epidemiologic and clinical characteristics. three patterns of yearly outbreaks existed: (1) strong predominance of group a strains (9 years with 83%-100% a strains), (2) relatively equal proportions of group a and b strains (4 years), and (3) strong predominance of group b strains (78%-85%) in 2 years, sepa ... | 1990 | 2230258 |
| rapid recovery in mice after combined nasal/oral immunization with killed respiratory syncytial virus. | based on the concept of a common mucosal immune system, the murine gastrointestinal tract was inoculated (oral) with three doses (5, 20, and 40 micrograms) of uv-inactivated respiratory syncytial virus (rsv) in order to elicit a virus-specific immune response in the respiratory tract. only the 40 micrograms dose induced significant (p less than 0.01) anti-rsv-igg rises in serum and lung wash compared to controls. to improve the immune response, mice were immunized intranasally under light anesth ... | 1990 | 2243208 |