Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| seroprevalence of varicella antibodies among pregnant women in lyon-france. | the purpose of the study was to calculate the seroprevalence of immunity to the varicella-zoster virus (vzv) infection and to evaluate the positive predictive value (ppv) and the negative predictive value (npv) of the self-reported history of vzv infection in pregnant women. a cross sectional study was conducted in 18 private medical analysis laboratories. information on socio-demographic characteristics and past history of varicella or zoster were collected using a questionnaire. blood samples ... | 2007 | 17534728 |
| simultaneous detection of three porcine viruses by multiplex pcr. | specific oligonucleotide primers were selected and combined in a multiplex arrangement, in order to detect simultaneously three economically important porcine viruses by polymerase chain reaction (pcr). the pathogen panel was comprised of viruses that cause reproductive failure in infected herds: aujeszky's disease virus (adv), porcine parvovirus (ppv) and porcine respiratory and reproductive syndrome virus (prrsv). in order to reduce the time required for the detection of the pathogens, the ass ... | 2007 | 17555292 |
| efficient priming against classical swine fever with a safe glycoprotein e2 expressing orf virus recombinant (orfv vrv-e2). | an increasing demand in livestock animal husbandry for intervention or emergency vaccination strategies requires a rapid onset of protection linked to prevention of infectious agent spread. using the new recombinant parapoxvirus (ppv) orf virus (orfv) as a vaccine expressing the csfv e2 glycoprotein we demonstrate that a single intra-muscular application confers solid protection. in the prime only concept, multi-site application of the vector vaccine turned out to be superior to single-site appl ... | 2007 | 17600594 |
| assessment of the diversity and dynamics of plum pox virus and aphid populations in transgenic european plums under mediterranean conditions. | the molecular variability of plum pox virus (ppv) populations was compared in transgenic european plums (prunus domestica l.) carrying the coat protein (cp) gene of ppv and non-transgenic plums in an experimental orchard in valencia, spain. a major objective of this study was to detect recombination between ppv cp transgene transcripts and infecting ppv rna. additionally, we assessed the number and species of ppv aphid vectors that visited transgenic and non-transgenic plum trees. test trees con ... | 2008 | 17605085 |
| evaluation of hepatic steatosis by ultrasound in patients with chronic hepatitis c virus infection. | to compare two alternative ultrasound parameters, hepatic vein flow (hvf) pattern and presence of focal hypoechoic areas (fha) within the liver hilus, as non-invasive predictors of liver steatosis in patients with chronic hepatitis c virus (hcv) infection. | 2007 | 17617117 |
| clinical utility of afp-l3% measurement in north american patients with hcv-related cirrhosis. | lens culinaris agglutinin-reactive fraction of alpha-fetoprotein (afp-l3%) has been reported to be useful in the early detection of hepatocellular carcinoma (hcc) in japan. the aim of this prospective study was to compare the clinical utility of afp-l3% with that of total afp in north american patients. | 2007 | 17617202 |
| a recombinant pseudorabies virus co-expressing capsid proteins precursor p1-2a of fmdv and vp2 protein of porcine parvovirus: a trivalent vaccine candidate. | pseudorabies (pr), foot-and-mouth disease (fmd), and porcine parvovirus disease are three important infectious diseases in swine worldwide. the gene-deleted pseudorabies virus (prv) has been used as a live-viral vector to develop multivalent genetic engineering vaccine. in this study, a recombinant prv, which could co-express protein precursor p1-2a of fmdv and vp2 protein of ppv, was constructed using prv tk(-)/ge(-)/lacz(+) mutant as the vector. after homologous recombination and plaque purifi ... | 2007 | 17628752 |
| simple blood tests as noninvasive markers of liver fibrosis in hemodialysis patients with chronic hepatitis c virus infection. | hcv infection is common among patients with end-stage renal disease (esrd) on hemodialysis, and it has been considered an independent risk factor for mortality in this setting. although liver biopsy in esrd patients with hcv infection is useful before kidney transplantation, it carries a high risk of complications. we sought to assess the diagnostic value of noninvasive markers to stage liver fibrosis in 203 esrd hcv-infected patients. univariate and multivariate analysis were used to identify v ... | 2007 | 17634962 |
| no influence of collagenous proteins of achilles tendon, skin and cartilage on the virus-inactivating efficacy of peracetic acid-ethanol. | the risk of transmitting human pathogenic viruses via allogeneic musculoskeletal tissue transplants is a problem requiring effective inactivation procedures. virus safety of bone transplants was achieved using peracetic acid (paa)-ethanol sterilisation. proteins are known to have an adverse effect on the virus-inactivating capacity of paa. therefore we investigated virus inactivation by paa in collagenous tissues. achilles tendon, skin and cartilage were cut into small pieces, lyophilised and co ... | 2007 | 17644408 |
| [efficacy of absorbance ratio of elisa antibodies [corrected] for hepatitis c virus of 3th generation in the prediction of viremia evaluated by pcr]. | in order to decrease the burden of suffering and the costs derived from confirmatory molecular assays, a better strategy is badly needed to decrease the rate of false positive results of the enzyme-linked immunoassay (elisa) for detection of hepatitis c virus (hcv) antibodies (anti). | 2007 | 17685198 |
| comparative expression profiling of nicotiana benthamiana leaves systemically infected with three fruit tree viruses. | plant viruses cause a wide array of disease symptoms and cytopathic effects. although some of these changes are virus specific, many appear to be common even among diverse viruses. currently, little is known about the underlying molecular determinants. to identify gene expression changes that are concomitant with virus symptoms, we performed comparative expression profiling experiments on nicotiana benthamiana leaves infected with one of three different fruit tree viruses that produce distinct s ... | 2007 | 17722703 |
| effectiveness of polysaccharide pneumococcal vaccine in hiv-infected patients: a case-control study. | polysaccharide pneumococcal vaccine (ppv) is recommended among human immunodeficiency virus (hiv)-infected patients, although its effect in reducing the incidence of pneumonia or invasive pneumococcal disease is not well established. our objective was to determine the effectiveness of 23-valent ppv in hiv-infected adults and the risk factors for pneumococcal pneumonia or invasive pneumococcal disease. | 2007 | 17806042 |
| role of myzus persicae (hemiptera: aphididae) and its secondary hosts in plum pox virus propagation. | plum pox virus (family potyviridae, genus potyvirus, ppv) is one of the most important viral pathogens of plants in the genus prunus, particularly prunus persica l. the role of the myzus persicae (sulzer) (hemiptera: aphididae) as a vector of ppv-m, and its role in spreading ppv-m, was investigated. ppv-m-infected peach trees were used as inoculum sources, and transmission to 15 herbaceous species commonly present in and around peach orchards was evaluated. the presence of ppv-m in secondary hos ... | 2007 | 17849850 |
| adaptation of plum pox virus to a herbaceous host (pisum sativum) following serial passages. | plum pox virus (ppv) populations from peaches are able to adapt consistently to herbaceous hosts, characterized by a reduction in time to symptom development, increases in inoculation efficiency and increased titres. ppv adaptation was studied by using pea (pisum sativum) as an alternative host. two isolates of ppv from peaches were inoculated and passaged in peas ten times using either aphid or mechanical inoculation, generating four independent passage lines. mechanical-transmission efficiency ... | 2007 | 17872538 |
| a chimeric plum pox virus shows reduced spread and cannot compete with its parental wild-type viruses in a mixed infection. | the effect of a recombination event in the genomic 3' end on the biological properties and competitiveness of plum pox virus (ppv) was investigated. therefore, a fragment spanning the coat protein (cp) coding region and a part of the 3' non-translated region of a non-aphid-transmissible strain of ppv (ppv-nat) was replaced by the corresponding region of a ppv sour cherry isolate (ppv-soc). the resulting chimera (ppv-nat/soc) caused severe symptoms in nicotiana benthamiana, resembling those of pp ... | 2007 | 17872539 |
| analysis of the complete genome of peach chlorotic mottle virus: identification of non-aug start codons, in vitro coat protein expression, and elucidation of serological cross-reactions. | the entire genome of peach chlorotic mottle virus (pcmv), originally identified as prunus persica cv. agua virus (4n6), was sequenced and analysed. pcmv cross-reacts with antisera to diverse viruses, such as plum pox virus (ppv), genus potyvirus, family potyviridae; and apple stem pitting virus (aspv), genus foveavirus, family flexiviridae. the pcmv genome consists of 9005 nucleotides (nts), excluding a poly(a) tail at the 3' end of the genome. five open reading frames (orfs) were identified wit ... | 2007 | 17891331 |
| electrophoretic mobility of the capsid protein of the plum pox virus strain ppv-rec indicates its partial phosphorylation. | a double-band sds-page profile was found reproducible for capsid protein (cp) of plum pox virus (ppv) isolates belonging to the strain ppv-rec. the double-band was also present in the virus population multiplied in various plants. a single-lesion passage in a hypersensitive host chenopodium foetidum showed that its presence was not a result of a mixed infection. we found that the two electrophoretic forms of cp shared identical n-terminus. therefore, they did not originate from an alternative pr ... | 2007 | 17900221 |
| gamma-glutamyltransferase and rapid virological response as predictors of successful treatment with experimental or standard peginterferon-alpha-2b in chronic hepatitis c non-responders. | high-dose peginterferon-alpha (pegifn-alpha) induction and prolongation of therapy may be an option to improve sustained virological response (svr) rates among hepatitis c virus (hcv) non-responders, although a higher and a longer dosing of pegifn-alpha may intensify side effects. | 2007 | 17919233 |
| oligonucleotide microarray-based detection and genotyping of plum pox virus. | plum pox virus (ppv) is the most damaging viral pathogen of stone fruits. the detection and identification of its strains are therefore of critical importance to plant quarantine and certification programs. existing methods to screen strains of ppv suffer from significant limitations such as the simultaneous detection and genotyping of several strains of ppv in samples infected with different isolates of the virus. a genomic strategy for ppv screening based on the viral nucleotide sequence was d ... | 2008 | 17920703 |
| demonstration of an association between detection of igg antibody reactivity towards the c-terminal region of the pres1 protein of hepatitis b virus and the capacity to respond to interferon therapy in chronic hepatitis b. | the treatment of hepatitis b virus (hbv) remains complex, with somewhat unpredictable responses. the aim of this study was to determine the predictive value of the pretreatment presence of circulatory antibodies towards a synthetic peptide mimicking the amino acids 94-117 of the pres1 protein of hbv and the capacity to respond to alpha-inteferon (ifn-alpha) treatment. | 2008 | 17931371 |
| [co-expression of csfv t cell epitope e290 peptide and ppv vp2 protein in lactobacillus casei and determination of specific antibodies in immunized mice]. | lactobacillus casei strain 393 was selected as an antigen delivery vehicle for the development of oral vaccine to express recombinant classical swine fever virus (csfv) t cell epitope e290 peptide and porcine parvovirus (ppv) vp2 protein. the recombinant genes encoding csfv t cell epitope e290 peptide and ppv vp2 protein, respectively, were cloned into the secretion expression vector ppg, and then the ppg-vp2-e290 was electrotransformed into l. casei 393 giving rise to recombinant strain ppg-vp2 ... | 2007 | 17944369 |
| development of a novel hot-start multiplex pcr for simultaneous detection of classical swine fever virus, african swine fever virus, porcine circovirus type 2, porcine reproductive and respiratory syndrome virus and porcine parvovirus. | a novel hot-start multiplex pcr (mpcr) assay was developed and subsequently evaluated for its effectiveness in simultaneously detecting multiple viral infections of swine. specific primers for each of five virus genomes, namely classical swine fever virus (csfv), african swine fever virus (asfv), porcine circovirus type 2 (pcv2), porcine reproductive and respiratory syndrome virus (prrsv) and porcine parvovirus (ppv) were used. combined nucleic acid purification was carried out using a commercia ... | 2008 | 17975735 |
| no recombination detected in artificial potyvirus mixed infections and between potyvirus derived transgenes and heterologous challenging potyviruses. | risk-assessment studies of virus-resistant transgenic plants (vrtps) focussing on recombination of a plant virus with a transgenic sequence of a different virus should include a comparison of recombination frequencies between viruses in double-infected non-transgenic plants with those observed in singly infected transgenic plants to estimate recombination incidence in vrtps. in this study, the occurrence of recombination events was investigated in non-transgenic plants double-infected with two d ... | 2007 | 18001687 |
| specific and efficient cleavage of fusion proteins by recombinant plum pox virus nia protease. | site-specific proteases are the most popular kind of enzymes for removing the fusion tags from fused target proteins. nuclear inclusion protein a (nia) proteases obtained from the family potyviridae have become promising due to their high activities and stringencies of sequences recognition. nia proteases from tobacco etch virus (tev) and tomato vein mottling virus (tvmv) have been shown to process recombinant proteins successfully in vitro. in this report, recombinant ppv (plum pox virus) nia p ... | 2008 | 18024078 |
| identification of plum pox virus pathogenicity determinants in herbaceous and woody hosts. | plum pox virus (ppv) is a member of the genus potyvirus that is able to infect a large variety of plant species, including trees of the genus prunus, its natural host. when some ppv isolates are propagated for an extended time in herbaceous plants, their ability to infect trees is reduced. the molecular basis of this change in host infectivity is poorly understood. we report the construction of hybrid viruses from cdna clones of two d-strain isolates of ppv, ppv-d and ppv-r, which differ in thei ... | 2008 | 18052879 |
| identification of liver fibrosis in hiv/hcv-coinfected patients using a simple predictive model based on routine laboratory data. | we constructed noninvasive models to predict significant fibrosis (f > or = 2) and advanced fibrosis (f > or = 3) among human immunodeficiency virus (hiv)/hepatitis c virus (hcv)-coinfected patients, naïve for anti-hcv treatment. a total of 296 patients with liver biopsy were randomly assigned to an estimation group (eg = 226; 70%) and a validation group (vg = 70; 30%). we developed the hospital gregorio marañón (hgm)-1 index, based on platelet count, aspartate aminotransferase (ast) and glucose ... | 2007 | 18070289 |
| role of the 25-26 nt sirna in the resistance of transgenic prunus domestica graft inoculated with plum pox virus. | the reaction of a genetically engineered plum clone (c5) resistant to plum pox virus (ppv) by graft inoculation with the virus was evaluated. the resistance in this clone has been demonstrated to be mediated through post-transcriptional gene silencing (ptgs). a single c5 plant out of 30 plants inoculated with ppv m strain by double chip-budding showed mild diffuse mosaic 'sharka' symptom at the bottom section of the scion. the upper leaves of this ppv-infected c5 plant remained symptomless and t ... | 2008 | 18071892 |
| undetectable hepatitis c virus rna at week 4 as predictor of sustained virological response in hiv patients with chronic hepatitis c. | low baseline serum hepatitis c virus (hcv)-rna and clearance of viraemia at week 4 with pegylated interferon (pegifn) plus ribavirin therapy predict sustained virological response (svr) and enable a shorter duration of therapy in patients with chronic hepatitis c. it is unclear whether this applies to hiv/hcv-co-infected patients. | 2008 | 18090387 |
| development of latex turbidimetric immunoassay for rapid and sensitive detection of influenza virus. | for the rapid and sensitive detection of influenza a and b viruses, a latex turbidimetric immunoassay (ltia) was developed using latex reagents prepared by the sensitization of anti-influenza a or b monoclonal antibodies on latex particles. we measured the immunoreactivity of these latex reagents to influenza a and b viral antigens. the sensitivity and specificity of ltia and reverse transcription-polymerase chain reaction (rt-pcr) for the detection of these viruses in clinical specimens (96 nas ... | 2007 | 18154440 |
| recurrent hepatitis c after liver transplantation: on-treatment prediction of response to peginterferon/ribavirin therapy. | sustained virologic response (svr) in the treatment of recurrent hepatitis c virus (hcv) infection after liver transplantation (lt) remains suboptimal. we evaluated efficacy of pegylated interferon alfa (peg) and ribavirin (rbv) (peg/rbv) combination therapy in lt recipients with recurrent hcv and predictive values of rapid virological response (rvr) and early virologic response (evr). between january 2001 and october 2005, lt recipients with recurrent hcv were intended to be treated for 48 week ... | 2008 | 18161839 |
| performance of noninvasive markers for liver fibrosis is reduced in chronic hepatitis c with normal transaminases. | in chronic hepatitis c, biopsy is the gold standard for assessment of liver fibrosis. few studies investigated noninvasive markers of liver fibrosis in hepatitis c virus (hcv) patients with normal alanine aminotrasferase (nalt). eighty hcv patients with nalt and 164 hcv patients with elevated alanine aminotrasferase (ealt) who underwent a diagnostic liver biopsy were evaluated for ast-to-platelet ratio, forns' index, ast-to-alt ratio (aar), fibrotest and the recently proposed fibroindex, using l ... | 2008 | 18179453 |
| identification of quantitative trait loci controlling symptom development during viral infection in arabidopsis thaliana. | in compatible interactions between plants and viruses that result in systemic infection, symptom development is a major phenotypic trait. however, host determinants governing this trait are mostly unknown, and the mechanisms underlying it are still poorly understood. in a previous study on the arabidopsis thaliana-plum pox virus (ppv) pathosystem, we showed a large degree of variation in symptom development among susceptible accessions. in particular, cvi-1 (cape verde islands) accumulates viral ... | 2008 | 18184064 |
| role of rapid virological response in prediction of sustained virological response to peg-ifn plus ribavirin in hcv / hiv co-infected individuals. | the objective of the study was to evaluate the role of rapid virological response (rvr) in predicting sustained virological response (svr) rates to hepatitis c virus (hcv) therapy. 65 hiv / hcv co-infected patients commenced hcv treatment per protocol. hiv / hcv patients with a mean cd4 count of 502 were treated for 24-48 weeks depending on genotype. virological response was assessed at weeks 4 (rvr), 12 [early virological response (evr)], 24, at end of treatment (eotr) and 24 weeks post-complet ... | 2008 | 18221297 |
| tuberculin skin test results in hiv-infected patients in india: implications for latent tuberculosis treatment. | to evaluate the utility of the tuberculin skin test (tst) in detecting latent and active tuberculosis (tb) among human immunodeficiency virus (hiv) infected patients in south india. | 2008 | 18230249 |
| optimized cutoffs improve performance of the aspartate aminotransferase to platelet ratio index for predicting significant liver fibrosis in human immunodeficiency virus/hepatitis c virus co-infection. | to assess the diagnostic value of modified cutoffs for aspartate aminotransferase to platelet ratio index (apri) to predict significant liver fibrosis in human immunodeficiency virus (hiv)/hepatitis c virus (hcv) patients. | 2008 | 18339075 |
| optimizing screening for acute human immunodeficiency virus infection with pooled nucleic acid amplification tests. | recent studies have shown the public health importance of identifying individuals with acute human immunodeficiency virus infection (ahi); however, the cost of nucleic acid amplification testing (naat) makes individual testing of at-risk individuals prohibitively expensive in many settings. pooled naat (or group testing) can improve efficiency and test performance of testing for ahi, but optimizing the pooling algorithm can be difficult. we developed simple, flexible biostatistical models of spe ... | 2008 | 18353930 |
| rapid detection of porcine circovirus type 2 by loop-mediated isothermal amplification. | a method of loop-mediated isothermal amplification (lamp) was employed to develop a rapid and simple detection system for porcine circovirus type 2 (pcv2). the amplification could be finished in 60 min under isothermal condition at 64 degrees c by employing a set of four primers targeting the cap gene of pcv2. the lamp assay showed higher sensitivity than the conventional pcr, with a detection limit of five copies per tube of purified pcv2 genomic dna. no cross-reactivity was observed from the s ... | 2008 | 18355932 |
| phylogenetic analysis of members of the phycodnaviridae virus family, using amplified fragments of the major capsid protein gene. | algal viruses are considered ecologically important by affecting host population dynamics and nutrient flow in aquatic food webs. members of the family phycodnaviridae are also interesting due to their extraordinary genome size. few algal viruses in the phycodnaviridae family have been sequenced, and those that have been have few genes in common and low gene homology. it has hence been difficult to design general pcr primers that allow further studies of their ecology and diversity. in this stud ... | 2008 | 18359826 |
| development of recombinant porcine parvovirus-like particles as an antigen carrier formed by the hybrid vp2 protein carrying immunoreactive epitope of porcine circovirus type 2. | virus-like particles (vlps) are non-replicative vectors for delivery of heterologous epitopes and induction of immune responses. in this study, a self-assembled porcine parvovirus (ppv) vp2 capsid protein [ppv:vlp-(pcv2)] carrying immunoreactive epitopes, residues 165-200 from the porcine circovirus 2 (pcv2) virus nucleoprotein was constructed. immunogenicity study was carried out with hybrid vlps derived from hek-293 cells infected with recombinant adenovirus vectors. to our knowledge, this stu ... | 2008 | 18378364 |
| des-gamma-carboxyprothrombin, alpha-fetoprotein and afp-l3 in patients with chronic hepatitis, cirrhosis and hepatocellular carcinoma. | hepatocellular carcinoma (hcc) is a common complication in patients with chronic viral hepatitis. detection of hcc at an early stage is critical for a favorable clinical outcome. the study aim was to: (i) compare the levels of des-gamma-carboxyprothrombin (dcp), alpha-fetoprotein (afp) and afp-l3 in hcc patients and in chronic viral hepatitis patients without hcc; (ii) define the level of each tumor marker with the best sensitivity and specificity for hcc diagnosis; and (iii) to correlate the le ... | 2008 | 18422961 |
| epstein-barr virus (ebv)-encoded rna: automated in-situ hybridization (ish) compared with manual ish and immunohistochemistry for detection of ebv in pediatric lymphoproliferative disorders. | detection of epstein-barr virus (ebv) may be achieved by various methods, including ebv-encoded rna (eber) in-situ hybridization (ish) and immunohistochemistry (ihc) for latent membrane protein (lmp-1). we compared novel automated ish and ihc techniques in pediatric lymphoproliferative disorders with results obtained by manual ish. thirty-seven pediatric cases previously studied by manual eber ish (including 18 eber-positive, 15 eber-negative, and 4 eber-equivocal cases) were used for the study. ... | 2009 | 18442302 |
| impact of pneumococcal vaccination on the incidence of pneumonia by hiv infection status among patients enrolled in the veterans aging cohort 5-site study. | human immunodeficiency virus (hiv)-infected persons have a high incidence of pneumonia and pneumococcal disease. benefits of vaccination with the 23-valent pneumococcal polysaccharide vaccine (ppv) among these patients continue to be debated. | 2008 | 18444830 |
| the predictive value of cerebrospinal fluid epstein-barr viral load as a marker of primary central nervous system lymphoma in hiv-infected persons. | the presence of epstein-barr virus (ebv) dna in cerebrospinal fluid (csf) is used as a marker of hiv-associated primary central nervous system lymphoma (pcnsl). in our setting, ebv dna is frequently detected in the csf of hiv-infected patients with miscellaneous neurological diseases and thus its presence is a poor predictor of pcnsl. | 2008 | 18455472 |
| evaluation of commercial ebv recombline assay for diagnosis of nasopharyngeal carcinoma. | in recent years a number of epstein-barr virus (ebv) proteins were defined as being immunodominant for either igm, igg or iga immune responses, yielding promising markers for diagnostic serology. specific reactivity patterns to these proteins have been described for infectious mononucleosis (im), nasopharyngeal carcinoma (npc), various types of lymphoma, and healthy ebv carriers. | 2008 | 18455473 |
| [meta-analysis for the pooled sensitivity and specificity of hepatitis b surface antigen rapid tests]. | although hepatitis b surface antigen (hbsag) rapid test based on immunochromatographic assay (ica) is now widely used, the test has not been evaluated sufficiently enough to validate its performance. thus, it is important to summarize the clinical performance of the test kits. in this study, we performed meta-analysis for the performance of the hbsag rapid tests. | 2008 | 18458514 |
| evaluation of the performance of four rapid tests for detection of hepatitis b surface antigen in antananarivo, madagascar. | four rapid immunochromatographic assays--determine hbsag, virucheck hbsag, cypress hbsag dipstick and hexagon hbsag--for human hepatitis b surface antigen (hbsag) detection in human serum were evaluated. a collection of reference serum samples (91 hbsag positive and 109 hbsag negative) stored at -80 degrees c was used. sensitivity and positive predictive value (ppv) exceeded 95%, and specificity and negative predictive value (npv) exceeded 96% for all tests. the determine hbsag test performed be ... | 2008 | 18462816 |
| identification of trimeric peptides that bind porcine parvovirus from mixtures containing human blood plasma. | virus contamination in human therapeutics is of growing concern as more therapeutic products from animal or human sources come into the market. all biopharmaceutical processes are required to have at least two distinct viral clearance steps to remove viruses. most of these steps work well for enveloped viruses and large viruses, whether enveloped or not. that leaves a class of small non-enveloped viruses, like parvoviruses and hepatitis a, which are not easily removed by these typical steps. in ... | 2008 | 18470993 |
| rapid immunoassay for the detection of genital herpes infection. | we evaluated a new affinity membrane strip test for the diagnosis of herpetic genital infections. test strip results, which are available by immunoassay in 30 min without the need for special equipment, were compared with the results of viral culture. | 1994 | 18475363 |
| performance characteristics of a new rapid immunochromatographic test for the detection of antibodies to human immunodeficiency virus (hiv) types 1 and 2. | diagnostic kits for the detection of human immunodeficiency virus (hiv) antibodies have reached an unprecedented number. but choice of an ideal, cost-effective, and rapid test for hiv infection is of immense value for use in developing countries like india, where resources are limited. in this study we have evaluated the performance characteristics of the rapid immunochromatographic hiv test kit first response hiv 1-2.o. first, the laboratory archived 450 characterized plasma/serum specimens, wh ... | 2008 | 18484656 |
| monitoring of bk virus replication in the first year following renal transplantation. | bk virus-associated nephropathy (bkvan) is one of the most common viral diseases affecting renal allografts. screening for viral replication may allow for earlier intervention with reduced allograft loss. a plasma viral load >10(4) copies/ml of bkv dna is recommended for a presumed diagnosis of bkvan. | 2008 | 18503098 |
| alteration in the chloroplastic metabolism leads to ros accumulation in pea plants in response to plum pox virus. | in this work, a recombinant plum pox virus (ppv, sharka) encoding green fluorescent protein is used to study its effect on antioxidant enzymes and protein expression at the subcellular level in pea plants (cv. alaska). ppv had produced chlorotic spots as well as necrotic spots in the oldest leaves at 13-15 d post-inoculation. at 15 d post-inoculation, ppv was present in the chlorotic and necrotic areas, as shown by the fluorescence signal produced by the presence of the green fluorescent protein ... | 2008 | 18535298 |
| molecular basis for porcine parvovirus detection in dead fetuses. | reproductive failures are still common grounds for complaint by commercial swine producers. porcine parvovirus (ppv) is associated with different clinical reproductive signs. the aim of the present study was to investigate ppv fetal infection at swine farms having ongoing reproductive performance problems. the presence of virus in fetal tissues was determined by nested-polymerase chain reaction assay directed to the conserved ns1 gene of ppv in aborted fetuses, mummies and stillborns. fetuses sh ... | 2008 | 18561384 |
| plum pox virus variability detected by the advanced analytical methods. | plum pox virus (ppv) infects stone-fruit trees with important economical impact mainly in europe and mediterranean region. the data about ppv intra-species variability accumulated markedly in the last two decades. six ppv strains have been recognized using different approaches including serology, protein analysis, specific amplification, and genome sequencing. reliable and sensitive diagnostics is the most important requirement for application of early control and safety measures. therefore, man ... | 2008 | 18564894 |
| the prehistory of potyviruses: their initial radiation was during the dawn of agriculture. | potyviruses are found world wide, are spread by probing aphids and cause considerable crop damage. potyvirus is one of the two largest plant virus genera and contains about 15% of all named plant virus species. when and why did the potyviruses become so numerous? here we answer the first question and discuss the other. | 2008 | 18575612 |
| altered gene expression changes in arabidopsis leaf tissues and protoplasts in response to plum pox virus infection. | virus infection induces the activation and suppression of global gene expression in the host. profiling gene expression changes in the host may provide insights into the molecular mechanisms that underlie host physiological and phenotypic responses to virus infection. in this study, the arabidopsis affymetrix ath1 array was used to assess global gene expression changes in arabidopsis thaliana plants infected with plum pox virus (ppv). to identify early genes in response to ppv infection, an arab ... | 2008 | 18613973 |
| dry-heat treatment process for enhancing viral safety of an antihemophilic factor viii concentrate prepared from human plasma. | viral safety is a prerequisite for manufacturing clinical antihemophilic factor viii concentrates from human plasma. with particular regard to the hepatitis a virus (hav), a terminal dry-heat treatment (100 degrees for 30 min) process, following lyophilization, was developed to improve the virus safety of a solvent/detergent-treated antihemophilic factor viii concentrate. the loss of factor viii activity during dry-heat treatment was of about 5%. no substantial changes were observed in the physi ... | 2008 | 18633304 |
| improvement of virus safety of an antihemophilc factor ix by virus filtration process. | viral safety is an important prerequisite for clinical preparations of plasma-derived pharmaceuticals. one potential way to increase the safety of therapeutic biological products is the use of a virus-retentive filter. in order to increase the viral safety of human antihemophilic factor ix, particularly in regard to non-enveloped viruses, virus removal process using a polyvinylidene fluoride membrane filter (viresolve nfp) has been optimized. the most critical factor affecting the filtration eff ... | 2008 | 18667862 |
| identification of restriction endonuclease with potential ability to cleave the hsv-2 genome: inherent potential for biosynthetic versus live recombinant microbicides. | herpes simplex virus types 1 and 2 are enveloped viruses with a linear dsdna genome of approximately 120-200 kb. genital infection with hsv-2 has been denoted as a major risk factor for acquisition and transmission of hiv-1. developing biomedical strategies for hsv-2 prevention is thus a central strategy in reducing global hiv-1 prevalence. this paper details the protocol for the isolation of restriction endunucleases (reases) with potent activity against the hsv-2 genome and models two biomedic ... | 2008 | 18687114 |
| hepatitis vaccination in patients with hepatitis c: practice and validation of codes at a large veterans administration medical centre. | hepatitis vaccination is recommended in patients with chronic liver disease. | 2008 | 18691350 |
| host-specific effect of p1 exchange between two potyviruses. | the potyviruses plum pox virus (ppv) and tobacco vein mottling virus (tvmv) have distinct host ranges and induce different symptoms in their common herbaceous hosts. to test the relevance of the p1 protein in host compatibility and pathogenicity, hybrid viruses were constructed in which the p1 coding sequence of ppv was completely or partially replaced by the corresponding sequences from tvmv. infections induced by these chimeric viruses revealed that the tvmv p1 and a ppv/tvmv hybrid p1 protein ... | 2008 | 18705848 |
| use of luminex xmap-derived bio-plex bead-based suspension array for specific detection of ppv w and characterization of epitopes on the coat protein of the virus. | a panel of monoclonal antibodies (mabs) directed against the n-terminus region of the coat protein (cp) of strain ppv w (isolate 3174) was generated by immunizing mice with recombinant peptides. the best performing mabs were identified as 2c3 and 10g7. mab 2c3 was selected for comparison of a standard tas-elisa protocol with a luminex xmap technology-derived bead-based suspension array system described as a triple antibody sandwich-microsphere immunoassay (tas-mia). tas-mia was as sensitive as t ... | 2008 | 18722476 |
| consensus recommendations on immunization, 2008. | justification: there is a need to formulate recommendations regarding use of new vaccines which have become recently available/will soon be available and to review/revise recommendations about existing vaccines in light of recent information. | 2008 | 18723905 |
| performance of indirect immunofluorescence assay, immunochromatography assay and reverse transcription-polymerase chain reaction for detecting human respiratory syncytial virus in nasopharyngeal aspirate samples. | comparison of the use of indirect immunofluorescence assay (ifa), immunochromatography assay (ica-bd) and reverse transcription-polymerase chain reaction (rt-pcr) for detecting human respiratory syncytial virus (hrsv) in 306 nasopharyngeal aspirates samples (npa) was performed in order to assess their analytical performance. by comparing the results obtained using ica-bd with those using ifa, we found relative indices of 85.0% for sensitivity and 91.2% for specificity, and the positive (ppv) and ... | 2008 | 18797759 |
| a temperature-controlled amplicon system derived from plum pox potyvirus. | the control of replication can facilitate a viral amplicon to reach high expression levels by enabling the virus to escape host defence mechanisms and reducing the deleterious effects of viral infection. we have developed a novel system to regulate amplicon expression by controlling the temperature of plant growth. nicotiana benthamiana plants were transformed at two different temperatures with a cdna copy of the plum pox potyvirus genome harbouring the open reading frame 2 of porcine circovirus ... | 2009 | 18801011 |
| plum pox in north america: identification of aphid vectors and a potential role for fruit in virus spread. | abstract thirteen aphid species were tested for their ability to transmit pennsylvania isolates of plum pox virus (ppv) collected in columbia (penn-3), franklin (penn-4), and york (penn-7) counties, pa. four species, aphis fabae, a. spiraecola, brachycaudus persicae, and myzus persicae, consistently transmitted ppv in preliminary transmission tests. two species, metopolophium dirhodum and rhopalosiphum padi, were occasional inefficient vectors. toxoptera citricida, from florida, also was an effe ... | 2004 | 18943108 |
| spatial pattern analysis of sharka disease (plum pox virus strain m) in peach orchards of southern france. | abstract the spatial pattern of sharka disease, caused by plum pox virus (ppv) strain m, was investigated in 18 peach plots located in two areas of southern france. ppv infections were monitored visually for each individual tree during one to three consecutive years. point pattern and correlation-type approaches were undertaken using the binary data directly or after parsing them in contiguous quadrats of 4, 9, and 16 trees. ordinary runs generally revealed a low but variable proportion of rows ... | 2003 | 18943618 |
| factors affecting the spread of plum pox virus strain m in peach orchards subjected to roguing in france. | abstract we evaluated the impact of roguing on the spread and persistence of the aggressive plum pox virus strain m (ppv-m) in 19 peach orchard blocks in southern france. during a 7- to 10-year period, orchards were visually inspected for ppv symptoms, and symptomatic trees were removed every year. disease incidence was low in all orchards at disease discovery and was <1% in 16 of the 19 orchard blocks. the spread of sharka disease was limited in all 19 blocks, with an annual disease incidence b ... | 2004 | 18943711 |
| identification of plum pox virus determinants implicated in specific interactions with different prunus spp. | abstract the characterization of pathogenic properties of two infectious clones of plum pox virus (ppv) isolates, pgppv (d group) and pgppvps (m group), was investigated in their woody hosts (seedlings of prunus spp.). the two clones differed in their ability to infect plum and peach cultivars, from no infection to local and systemic infection. the phenotype determinants were located with a set of chimeric viruses from the two clones. in plum, determinants of systemic infection were located in a ... | 2001 | 18944389 |
| a single amino acid mutation in the plum pox virus helper component-proteinase gene abolishes both synergistic and rna silencing suppression activities. | abstract the effects on symptom expression of single amino acid mutations in the central region of the plum pox virus (ppv) helper component-proteinase (hc-pro) gene were analyzed in nicotiana benthamiana using potato virus x (pvx) recombinant viruses. pvx recombinant virus expressing the wild-type variant of ppv hc-pro induced the expected enhancement of pvx pathogenicity, manifested as necrosis and plant death. recombinant virus expressing a variant of ppv hc-pro containing a single point muta ... | 2005 | 18944411 |
| interference between d and m types of plum pox virus in japanese plum assessed by specific monoclonal antibodies and quantitative real-time reverse transcription-polymerase chain reaction. | abstract the dynamics of virus interference between two isolates of plum pox virus (ppv) belonging to the main ppv types, d and m, were analyzed in japanese plum (prunus salicina) by challenge inoculations. to assess the consequences of a ppv-m infection on plum already infected with ppv-d, and vice versa (predominance of one of the strains, recombination, synergism, symptoms aggravation, and so on), 30 japanese plum trees were graft inoculated with ppv-d or ppv-m isolates in quarantine conditio ... | 2006 | 18944448 |
| comparison of monoclonal antibodies and polymerase chain reaction assays for the typing of isolates belonging to the d and m serotypes of plum pox potyvirus. | abstract plum pox potyvirus (ppv) isolates may be divided into four groups separated by serological, molecular, and epidemiological differences. monoclonal antibodies specific for the two major groups of isolates, represented by the d and m serotypes of the virus, have been obtained. polymerase chain reaction (pcr)-based assays allowing the direct detection and differentiation of ppv isolates have also been developed. we now report on a large-scale comparison of these two typing approaches. the ... | 1998 | 18944965 |
| an evaluation of saliva as an alternative to plasma for the detection of hepatitis c virus antibodies. | seroepidemiological studies on the prevalence of hepatitis c virus (hcv) in india have been hampered by reluctance of subjects to provide blood samples for testing. we evaluated the use of saliva as an alternate specimen to blood for the detection of antibodies to hcv. | 2008 | 18974484 |
| liver stiffness in the hepatitis b virus carrier: a non-invasive marker of liver disease influenced by the pattern of transaminases. | to investigate the usefulness of transient elastography by fibroscan (fs), a rapid non-invasive technique to evaluate liver fibrosis, in the management of chronic hepatitis b virus (hbv) carriers. | 2008 | 18985805 |
| detection of porcine parvovirus by loop-mediated isothermal amplification. | loop-mediated isothermal amplification is a novel method for rapid amplification of dna. it has been adopted widely for the detection of virus because of its simplicity, rapidity, and specificity. a loop-mediated isothermal amplification assay was developed for the detection of porcine parvovirus. four primers specific for six regions of ppv non-structural protein 1 gene were designed with an online software. after amplifying at a constant temperature of 59-65 degrees c by bst enzyme, a clear re ... | 2009 | 18996150 |
| comparison of the liver stiffness measurement by transient elastography with the liver biopsy. | to compare the liver stiffness (ls) measurement by transient elastography (te) to the liver biopsy (lb)-considered the "gold standard" in the evaluation of patients with chronic hepatitis c. | 2008 | 19030204 |
| endocervical cytobrush for the detection of cervical dysplasia before large loop excision of the transformation zone (lletz). | we aimed to identify the number of histologically verified cervical intraepithelial neoplasia (cin)/cervical cancer lesions detected by endocervical cytobrush (ec) which have been missed by repeat pap smear and colposcopy before large loop excision of the transformation zone (lletz). | 2008 | 19031969 |
| real-time intraoperative detection of melanoma lymph node metastases using recombinant vaccinia virus glv-1h68 in an immunocompetent animal model. | there is a clinical need for improved intraoperative detection of lymph node metastases from malignant melanoma (mm). we aimed to investigate the use of recombinant vaccinia virus glv-1h68, expressing green fluorescent protein (gfp), for real-time intraoperative detection of melanoma lymph node metastases in an immunocompetent animal model. mice bearing foot pad tumors received intratumoral injections of glv-1h68, and 48 hr later were evaluated for popliteal lymph node metastasis using noninvasi ... | 2009 | 19035444 |
| characterization of a natural plum pox virus isolate bearing a truncated coat protein. | plum pox virus (ppv) isolates were collected in hungary from plum varieties. pcr targeting the 3' genomic region resulted in a shorter pcr product in the case of the b1298 isolate bearing a 135-nucleotide deletion in frame in the n-terminal part of the coat protein (cp). the isolate was aphid-transmissible and the virion diameter was reduced compared to ppv-sk68. detectability of this isolate by western blot varied according to the antibody used. integration of the deleted cp gene into an infect ... | 2009 | 19082685 |
| abbott realtime hepatitis c virus (hcv) and roche cobas ampliprep/cobas taqman hcv assays for prediction of sustained virological response to pegylated interferon and ribavirin in chronic hepatitis c patients. | two commercial real-time pcr assays are currently available for sensitive hepatitis c virus (hcv) rna quantification: the abbott realtime hcv assay (art) and roche cobas ampliprep/cobas taqman hcv assay (cap/ctm). we assessed whether the two real-time pcr assays were more effective than roche cobas amplicor hcv monitor test, v.2.0 (cam) for prediction of the sustained virological response (svr) to pegylated interferon (peg-ifn) plus ribavirin (rbv) in chronic hepatitis c. sixty patients chronica ... | 2009 | 19091819 |
| expression, purification, and characterization of recombinant ns-1, the porcine parvovirus non-structural protein. | the non-structural protein ns-1 of porcine parvovirus (ppv) could be a useful antigen for differentiating pigs infected with ppv from those vaccinated with inactivated whole virus. ns-1 was expressed in escherichia coli using the pet-32a (+) vector. the fusion protein, which was expressed at a high level, was similar antigenically to the native ns-1 protein as determined by western blot assay using polyclonal antibodies from pigs vaccinated with alive ppv vaccine. a simple procedure was used to ... | 2009 | 19101593 |
| early serum hbsag drop: a strong predictor of sustained virological response to pegylated interferon alfa-2a in hbeag-negative patients. | pegylated interferon alfa-2a (peg-ifn) may induce sustained virological response (svr) in 20% of hepatitis b e antigen (hbeag)-negative chronic hepatitis b (chb) patients. in addition, loss of hepatitis b surface antigen (hbsag) is achieved with a 10% yearly rate after treatment cessation in sustained responders. the aim of this study was to assess on-treatment serum hbsag kinetics to predict svr in hbeag-negative patients treated with peg-ifn. forty-eight consecutive patients were treated with ... | 2009 | 19115222 |
| evaluation of the who clinical case definition of aids among children in india. | the need of a clinical case definition (ccd) for acquired immunodeficiency syndrome (aids) was felt by public health agencies to monitor diseases resulting from human immunodeficiency virus (hiv) infection. to test the statistical significance of the existing world health organization (who) ccd for the diagnosis of aids in areas where diagnostic resources are limited in india, a prospective study was conducted in the paediatrics department at dr. ram manohar lohia hospital, new delhi. 360 cases ... | 2008 | 19127663 |
| compromised virus-induced gene silencing in rdr6-deficient plants. | rna silencing in plants serves as a potent antiviral defense mechanism through the action of small interfering rnas (sirnas), which direct rna degradation. sirnas can be derived directly from the viral genome or via the action of host-encoded rna-dependent rna polymerases (rdrs). plant genomes encode multiple rdrs, and it has been demonstrated that plants defective for rdr6 hyperaccumulate several classes of virus. in this study, we compared the effectiveness of virus-induced gene silencing (vig ... | 2009 | 19129420 |
| viral lysis of phaeocystis pouchetii: implications for algal population dynamics and heterotrophic c, n and p cycling. | a model ecosystem with two autotrophic flagellates, phaeocystis pouchetii and rhodomonas salina, a virus specific to p. pouchetii (ppv) and bacteria and heterotrophic nanoflagellates was used to investigate effects of viral lysis on algal population dynamics and heterotrophic nitrogen and phosphorus mineralization. lysis of p. pouchetii by ppv had strong positive effects on bacterial and hnf abundance, and the mass balance of c, n and p suggested an efficient transfer of organic material from p. ... | 2009 | 19129863 |
| simultaneous detection of porcine circovirus type 2, classical swine fever virus, porcine parvovirus and porcine reproductive and respiratory syndrome virus in pigs by multiplex polymerase chain reaction. | a multiplex polymerase chain reaction (pcr) was designed for the simultaneous detection of four viruses involved in reproductive and respiratory failure in pigs: porcine circovirus type 2 (pcv-2), porcine parvovirus (ppv), classical swine fever virus (csfv) and porcine reproductive and respiratory syndrome virus (prrsv). each of the four pairs of oligonucleotide primers exclusively amplified the targeted fragment of the specific viruses. the sensitivity of the multiplex pcr using purified plasmi ... | 2010 | 19131259 |
| serologic detection of herpes simplex virus type 2 antibodies among pregnant women using a point-of-care test from focus diagnostics. | serologic assays that identify herpes simplex type 2 (hsv-2) type-specific antibodies have been commercially available for more than a decade. greater acceptance of these tests is hindered by uncertainty regarding their performance in real-world clinical settings. | 2009 | 19131273 |
| [identification of porcine parvovirus and virus-specific antibodies by polymerase chain reaction and enzyme immunoassay]. | porcine parvovirus infection (ppvi) caused by a small non-enveloped virus (porcine parvovirus, ppv) is responsible for serious reproductive dysfunctions in sows. in the russian federation, the approved diagnostic methods for determination of parvovirus antigen and virus-specific antibodies are hemagglutination and hemagglutination-inhibition tests, respectively. the present paper gives the results of developing diagnostic tests to reveal porcine parvovirus by polymerase chain reaction and its an ... | 2008 | 19172907 |
| virodynamic predictors of response to pegylated interferon and lamivudine combination treatment of hepatitis b e antigen-positive chronic hepatitis b. | early identification of non-responders to interferon-alpha and development of stopping rules are needed in patients with chronic hepatitis b to reduce treatment-related costs and morbidity. | 2008 | 19195328 |
| a multiplex pcr for rapid and simultaneous detection of porcine circovirus type 2, porcine parvovirus, porcine pseudorabies virus, and porcine reproductive and respiratory syndrome virus in clinical specimens. | a multiplex pcr (mpcr) assay was developed and evaluated for its ability to simultaneously detect multiple viral infections of swine. specific primers were designed for each of the following four dna or rna viruses: porcine circovirus type 2 (pcv2), porcine parvovirus (ppv), pseudorabies virus (prv), and porcine reproductive and respiratory syndrome virus (prrsv). each target produced a specific amplicon with a size of 353 bp (pcv2), 271 bp (ppv), 194 bp (prv), or 434 bp (prrsv). the assay was s ... | 2009 | 19214728 |
| [technics of inactivating/removing medical chitosan pathogen]. | to verify the technics of inactivating/removing pathogens in medical chitosan derived from shrimp shell. | 2009 | 19275109 |
| predictors of smear-negative pulmonary tuberculosis in hiv-infected patients, battambang, cambodia. | retrospective data analysis of human immunodeficiency virus (hiv) infected patients attending an hiv clinic (referral hospital), cambodia. chest x-rays (cxrs) were read independently by onsite and offsite physicians. | 2009 | 19275795 |
| differentiation of plum pox virus isolates by single-strand conformation polymorphism and low-stringency single specific primer pcr analysis of hc-pro genome region. | single-strand conformation polymorphism (sscp) and low-stringency single specific primer (lssp)-pcr were assessed for suitability and reliability in genotyping of plum pox virus (ppv) isolates. examined ppv isolates included 16 ppv-d, 12 ppv-m, and 14 ppv-rec isolates collected in czech republic. the analysis was performed on the helper component protease (hc-pro) region of the ppv genome. sscp and lssp-pcr allowed the differentiation of ppv strain, but sscp was not able to distinguish isolates ... | 2009 | 19301952 |
| partial molecular characterization of an unusual, recombinant plum pox virus isolate from bulgaria. | 2009 | 19301955 | |
| comparison of cd4 cell count, viral load, and other markers for the prediction of mortality among hiv-1-infected kenyan pregnant women. | there are limited data regarding the relative merits of biomarkers as predictors of mortality or time to initiation of antiretroviral therapy (art). | 2009 | 19317628 |
| real-time pcr to detect and analyze virulent ppv loads in artificially challenged sows and their fetuses. | to establish a real-time polymerase chain reaction with sybr green for detection and quantification of porcine parvovirus (ppv) in porcine tissues, two primers specific for the non-structural protein 1 gene were designed. the detection limit of this assay was 3-23 gene copies/reaction, equivalent to 0.001 tcid(50)/ml. the assay was linear over a 10(6) dilution range of template concentrations. other porcine pathogens involved in reproductive disorders (porcine circovirus 2, porcine reproductive ... | 2009 | 19345523 |
| native electrophoresis and western blot analysis: method and applications. | native electrophoresis and western blot analysis (neweb) has been developed for the study of plant virus characteristics, among others, virus particle-protein interactions, electrophorotype formation, and strain separation. the method is based on the property of electrophoretic mobility of virus particles (vp) and proteins and combines the analytical capacity of electrophoresis with the specificity of western blot. one of its advantages is that it deals with entire vp that can be studied in caus ... | 2009 | 19378066 |
| quantitative trait loci meta-analysis of plum pox virus resistance in apricot (prunus armeniaca l.): new insights on the organization and the identification of genomic resistance factors. | plum pox virus (ppv) is responsible for sharka disease, one of the most detrimental stone fruit diseases affecting prunus trees worldwide. only a few apricot cultivars have been described as resistant, most originating from north american breeding programmes. several ppv resistance quantitative trait loci (qtls) have been mapped in various progenies, consistently highlighting the contribution to the resistance of the upper part of linkage group 1 (lg1). however, to date, no consensus has been re ... | 2009 | 19400838 |
| detection and phylogenetic analysis of orf virus from sheep in brazil: a case report. | orf virus (orfv), the prototype of the genus parapoxvirus (ppv), is the etiological agent of contagious ecthyma, a severe exanthematic dermatitis that afflicts domestic and wild small ruminants. although south american orfv outbreaks have occurred and diagnosed there are no south american ppv major membrane glycoprotein b2l gene nucleotide sequences available. | 2009 | 19413907 |
| rapid detection of porcine parvovirus dna by sensitive loop-mediated isothermal amplification. | a method of loop-mediated isothermal amplification (lamp) was employed to develop a rapid and simple detection system for porcine parvovirus (ppv) dna. the amplification could be finished in 45 min under isothermal condition at 62 degrees c by employing a set of four primers targeting vp2 gene of ppv. lamp assay showed higher sensitivity than pcr, with a detection limit of 5 copies of ppv genomic dna per reaction. no cross reactivity was observed from the samples of other related viruses includi ... | 2009 | 19428576 |
| further characterization of a new recombinant group of plum pox virus isolates, ppv-t, found in orchards in the ankara province of turkey. | sixteen plum pox virus (ppv) isolates collected in the ankara region of turkey were analyzed using available serological and molecular typing assays. surprisingly, despite the fact that all isolates except one, which was a mix infection, were typed as belonging to the ppv-m strain in four independent molecular assays, nine of them (60%) reacted with both ppv-m specific and ppv-d specific monoclonal antibodies. partial 5' and 3' genomic sequence analysis on four isolates demonstrated that irrespe ... | 2009 | 19428744 |