Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| puumala virus pulmonary syndrome in a romanian immigrant. | hantaviruses belong to the bunyaviridae family, which is comprised of bunyavirus, phlebovirus, nairovirus, and hantavirus. euroasiatic hantaviruses belong to two distinct subfamilies: murinae (comprising hantaan, dobrava, and seoul viruses), which are responsible for hemorrhagic fever with renal syndrome (hfrs), and arvicolinae (comprising puumala virus), responsible for nephropathia epidemica (ne) and hfrs. on the contrary, the new world hantavirus belongs to the sigmodontinae subfamily and inc ... | 2002 | 12962589 |
| isolation of kaeng khoi virus from dead chaerephon plicata bats in cambodia. | a virus isolated from dead chaerephon plicata bats collected near kampot, cambodia, was identified as a member of the family bunyaviridae by electron microscopy. the only bunyavirus previously isolated from chaerephon species bats in south-east asia is kaeng khoi (kk) virus (genus orthobunyavirus), detected in thailand over 30 years earlier and implicated as a public health problem. using rt-pcr, nucleotide sequences from the m rna segment of several virus isolates from the cambodian c. plicata ... | 2003 | 13679602 |
| epidemiological, clinical and laboratory aspects of sandfly fever. | sandfly fever viruses are still a significant health problem in many regions of the world, such as africa, the mediterranean basin, the middle east, and central asia. this review provides an update on the advances in knowledge about epidemiological, clinical, and laboratory aspects of infections caused by toscana, sicilian and naples viruses. | 2003 | 14501989 |
| inhibition of translation and induction of apoptosis by bunyaviral nonstructural proteins bearing sequence similarity to reaper. | members of the california serogroup of bunyaviruses (family bunyaviridae) are the leading cause of pediatric viral encephalitis in north america. significant cell death is observed as part of the infection pathology. we now report that a bunyaviral nonstructural protein termed nss shows sequence similarity to reaper, a proapoptotic protein from drosophila. although nss proteins lack the reaper n-terminal motif critical for iap inhibition, they do retain other functions of reaper that map to cons ... | 2003 | 14517326 |
| viral modulators of cell death provide new links to old pathways. | by observing how viruses facilitate their parasitic relationships with host cells, we gain insights into key regulatory pathways of the cell. not only are mitochondria key players in the regulation of programmed cell death, but many viral regulators of cell death also alter mitochondrial functions either directly or indirectly. although cytomegalovirus vmia and epstein-barr virus bhrf1 seem to have opposite effects on mitochondrial morphology, they both inhibit cell death. drosophila reaper, a r ... | 2003 | 14644194 |
| linear amplification followed by single primer polymerase chain reaction to amplify unknown dna fragments: complete nucleotide sequence of oropouche virus m rna segment. | the whole nucleotide sequence of oropouche virus medium (m) rna, orthobunyavirus genus, bunyaviridae family, was obtained using a new genomic amplification method. this method is based on the use of a single and specific primer of high melting temperature in a linear amplification (la), followed by a single primer polymerase chain reaction (lasp-pcr). the lasp-pcr was used to walk along the oropouche m rna completing the sequence in seven successive walks. the amplicons obtained in each walking ... | 2004 | 14656460 |
| inhibition of dugbe nairovirus replication by human mxa protein. | sensitivity to the interferon-induced protein, mxa, has previously been demonstrated for viruses belonging to the orthobunyavirus, hantavirus and phlebovirus genera of the bunyaviridae family. we have extended these findings to a member of the fourth and remaining genus containing viruses that infect man and other animals, the nairovirus dugbe virus (dugv). indirect immunofluorescence experiments using va9 cells (vero cells permanently transfected with mxa cdna) revealed strongly reduced dugv an ... | 2004 | 14687945 |
| bunyamwera bunyavirus rna synthesis requires cooperation of 3'- and 5'-terminal sequences. | bunyamwera virus (bunv) is the prototype of both the orthobunyavirus genus and the bunyaviridae family of segmented negative-sense rna viruses. the tripartite bunv genome consists of small (s), medium (m), and large (l) segments that are each transcribed to yield a single mrna and are replicated to generate an antigenome that acts as a template for synthesis of further genomic strands. as for all negative-sense rna viruses, the 3'- and 5'-terminal nontranslated regions (ntrs) of the bunv s, m, a ... | 2004 | 14722268 |
| prevalence of antibodies against saint louis encephalitis and jamestown canyon viruses in california horses. | jamestown canyon (jc) and saint louis encephalitis (sle) viruses are mosquito-transmitted viruses that have long been present in california. the objective of this study was to determine the seroprevalence of these two viruses in horses prior to the introduction of west nile (wn) virus. approximately 15% of serum samples collected in 1998 from 425 horses on 44 equine operations horses throughout california had serum antibodies to jc virus, whereas antibodies were not detected to sle virus. the re ... | 2004 | 15001316 |
| crimean-congo hemorrhagic fever virus genome l rna segment and encoded protein. | sequence analysis of the l rna genome segment and predicted encoded l polymerase protein of crimean-congo hemorrhagic fever (cchf) virus (genus nairovirus, family bunyaviridae) demonstrates that they are approximately twice the size of those found in viruses of other bunyavirus genera. the cchf virus l segment and encoded protein (12,164 nucleotides and 3944 amino acids, respectively) are similar in size and sequence to those of the nairovirus dugbe virus (12,255/62% and 4036/62% nucleotide and ... | 2004 | 15033562 |
| the emergence in japan of sathuperi virus, a tropical simbu serogroup virus of the genus orthobunyavirus. | in 1999, two viruses were isolated from blood samples of sentinel cattle in the western part of japan. the physiochemical and morphological properties of these viruses indicated that they belonged to the family bunyaviridae. sequence analysis of the s segment indicates that the two viruses are closely related to sathuperi virus (satv). the n-terminal 168 amino acid of the g2 protein of the m segment was highly homologous with that of satv (98.2%). given these results, we conclude that the newly ... | 2004 | 15098114 |
| serological evidence of akabane virus infection in northern israel in 2001. | in february 2002 the first cases of a "blind newborn calves" syndrome with hydranencephaly appeared in israel. eighty-one serum samples, from 54 animals on farms where the syndrome was recorded and 27 others from unaffected farms were examined by neutralization of akabane virus (akav, strain obe-1) by the micro-titer method. forty-seven of the 54 samples from the affected farms contained high serum neutralization titers against akav (mean sn titer 79.5 and +/- 44.7, standard deviation), whereas ... | 2004 | 15133276 |
| a bunyamwera virus minireplicon system in mosquito cells. | artificial minigenomes are powerful tools for studying the replication and transcription of negative-strand rna viruses. bunyamwera virus (bun; genus orthobunyavirus, family bunyaviridae) is an arbovirus that shows fundamental biological differences when replicating in mammalian versus mosquito cells. to study bun rna synthesis in mosquito cells, we developed a bacteriophage t7 rna polymerase-based minireplicon system similar to that described previously for mammalian cells. an aedes albopictus ... | 2004 | 15140965 |
| inhibition of rna polymerase ii phosphorylation by a viral interferon antagonist. | many viruses subvert the cellular interferon (ifn) system with so-called ifn antagonists. bunyamwera virus (bunv) belongs to the family bunyaviridae and is transmitted by arthropods. we have recently identified the nonstructural protein nss of bunv as a virulence factor that inhibits ifn-beta gene expression in the mammalian host. here, we demonstrate that nss targets the rna polymerase ii (rnap ii) complex. the c-terminal domain (ctd) of rnap ii consists of 52 repeats of the consensus sequence ... | 2004 | 15150262 |
| virus inactivation by nucleic acid extraction reagents. | many assume that common methods to extract viral nucleic acids are able to render a sample non-infectious. it may be that inactivation of infectious virus is incomplete during viral nucleic acid extraction methods. accordingly, two common viral nucleic acid extraction techniques were evaluated for the ability to inactivate high viral titer specimens. in particular, the potential for trizol ls reagent (invitrogen corp., carlsbad, ca) and avl buffer (qiagen, valencia, ca) were examined to render s ... | 2004 | 15158603 |
| simultaneous detection of bovine arboviruses using single-tube multiplex reverse transcription-polymerase chain reaction. | single-tube multiplex reverse transcription-polymerase chain reaction (mrt-pcr) assay was developed to detect and identify arboviruses in infected cell-culture fluids and field specimens. the technique was equally sensitive for detecting five different viruses in cell cultures, namely the chuzan, ibaraki, and bluetongue viruses belonging to orbivirus, and the akabane virus and peaton virus belonging to orthobunyavirus, and was less sensitive than former viruses for detecting aino virus belonging ... | 2004 | 15234812 |
| ngari virus is a bunyamwera virus reassortant that can be associated with large outbreaks of hemorrhagic fever in africa. | two isolates of a virus of the genus orthobunyavirus (family bunyaviridae) were obtained from hemorrhagic fever cases during a large disease outbreak in east africa in 1997 and 1998. sequence analysis of regions of the three genomic rna segments of the virus (provisionally referred to as garissa virus) suggested that it was a genetic reassortant virus with s and l segments derived from bunyamwera virus but an m segment from an unidentified virus of the genus orthobunyavirus. while high genetic d ... | 2004 | 15280501 |
| [investigation of arboviruses in lancang river down-stream area in yunnan province]. | to investigate the epidemic state of arboviruses in the downstream area of lancang river in yunnan province. | 2003 | 15340542 |
| missorting of lacrosse virus nucleocapsid protein by the interferon-induced mxa gtpase involves smooth er membranes. | the interferon-induced human mxa protein belongs to the class of dynamin-like, large guanosine-5'-triphosphatases that are involved in intracellular vesicle trafficking and organelle homeostasis. mxa shares many properties with the other members of this protein superfamily, including the propensity to self-assemble and to associate with lipid membranes. however, mxa is unique in that it has antiviral activity and inhibits the replication of several rna viruses. here, we determined the role of me ... | 2004 | 15355513 |
| analysis of the medium (m) segment sequence of guaroa virus and its comparison to other orthobunyaviruses. | guaroa virus (grov), a segmented virus in the genus orthobunyavirus, has been linked to the bunyamwera serogroup (bun) through cross-reactivity in complement fixation assays of s segment-encoded nucleocapsid protein determinants, and also to the california serogroup (cal) through cross-reactivity in neutralization assays of m segment-encoded glycoprotein determinants. phylogenetic analysis of the s-segment sequence supported a closer relationship to the bun serogroup for this segment and it was ... | 2004 | 15448370 |
| functional analysis of the noncoding regions of the uukuniemi virus (bunyaviridae) rna segments. | the role of the variable portion of the noncoding regions (ncrs) of the three bunyaviridae rna segments (l, m, s) in transcription, replication, and packaging was studied using the recently developed plasmid-driven rna polymerase i minigenome system for uukuniemi (uuk) virus, genus phlebovirus (11), as a model. comparison of the different segments showed that all ncrs were sufficient to mediate transcription/replication of a minigenome but demonstrated decreased promoter strength in the order m ... | 2004 | 15479814 |
| complementarity, sequence and structural elements within the 3' and 5' non-coding regions of the bunyamwera orthobunyavirus s segment determine promoter strength. | the genome of bunyamwera virus (bun; family bunyaviridae) consists of three segments of negative-sense, single-stranded rna that are called l (large), m (medium) and s (small), according to their size. the genomic rnas are encapsidated by the viral nucleocapsid protein to form ribonucleoprotein complexes (rnps). the terminal 3' and 5' non-coding sequences are complementary and interact to give a panhandle-like structure to the rnp. located within these non-coding sequences are elements that cont ... | 2004 | 15483240 |
| the resurgence of shamonda virus, an african simbu group virus of the genus orthobunyavirus, in japan. | six virus isolations were made from culicoides biting midges and blood samples of sentinel cattle in kagoshima and miyazaki prefectures, the southern part of japan, in 2002. serological and genetical tests identified these viruses as isolates of shamonda virus (shav), which belongs to the simbu group of the genus orthobunyavirus of the family bunyaviridae. initially, shav was isolated from cattle and culicoides biting midges in nigeria in the 1960s, and its presence has not been reported until t ... | 2005 | 15503217 |
| proteomics computational analyses suggest that the carboxyl terminal glycoproteins of bunyaviruses are class ii viral fusion protein (beta-penetrenes). | the bunyaviridae family of enveloped rna viruses includes five genuses, orthobunyaviruses, hantaviruses, phleboviruses, nairoviruses and tospoviruses. it has not been determined which bunyavirus protein mediates virion:cell membrane fusion. class ii viral fusion proteins (beta-penetrenes), encoded by members of the alphaviridae and flaviviridae, are comprised of three antiparallel beta sheet domains with an internal fusion peptide located at the end of domain ii. proteomics computational analyse ... | 2004 | 15544707 |
| oligomerization of hantavirus n protein: c-terminal alpha-helices interact to form a shared hydrophobic space. | the structure of the nucleocapsid protein of bunyaviruses has not been defined. earlier we have shown that tula hantavirus n protein oligomerization is dependent on the c-terminal domains. of them, the helix-loop-helix motif was found to be an essential structure. computer modeling predicted that oligomerization occurs via helix protrusions, and the shared hydrophobic space formed by amino acids residues 380-iillf-384 in the first helix and 413-li-414 in the second helix is responsible for stabi ... | 2004 | 15564476 |
| efficient bunyavirus rescue from cloned cdna. | bunyaviruses are trisegmented, negative-sense rna viruses. previously, we described a rescue system to recover infectious bunyamwera virus (genus orthobunyavirus) entirely from cloned cdna (bridgen, a. and elliott, r.m. (1996) proc. nat. acad. sci. usa 93, 15400-15404) utilizing a recombinant vaccinia virus expressing bacteriophage t7 rna polymerase to drive intracellular transcription of transfected t7 promoter-containing plasmids. here we report efforts to improve the efficiency of the system ... | 2004 | 15567443 |
| competitive enzyme-linked immunosorbent assay for the detection of the antibodies specific to akabane virus. | a competitive enzyme-linked immunosorbent assay (c-elisa) using neutralizing monoclonal antibodies (mabs) against akabane virus (akav) was developed to detect antibodies to akav in cattle sera. the performance of the test using 7 different competitor mabs was evaluated in sequential serum samples and sera from cattle infected with various bovine arboviruses. the dynamics of the antibody response expressed by percentage of inhibition (pi) in c-elisa coincided with those of neutralizing antibody t ... | 2004 | 15586574 |
| la crosse virus nonstructural protein nss counteracts the effects of short interfering rna. | through a process known as rna interference (rnai), double-stranded short interfering rnas (sirnas) silence gene expression in a sequence-specific manner. recently, several viral proteins, including the nonstructural protein nss of tomato spotted wilt virus (a plant-infecting bunyavirus), the interferon antagonist protein ns1 of influenza virus, and the e3l protein of vaccinia virus, have been shown to function as suppressors of rnai, presumably as a counterdefense against cellular mechanisms th ... | 2005 | 15596819 |
| hantaviruses: immunology, treatment, and prevention. | hantaviruses are rodent-borne bunyaviruses that are associated with two main clinical diseases in humans: hemorrhagic fever with renal syndrome and hantavirus pulmonary syndrome. it has been suggested that host-related immune mechanisms rather than direct viral cytopathology may be responsible for the principal abnormality (vascular dysfunction) in these syndromes. this review summarizes the current knowledge on hantaviral host immune responses, immune abnormalities, laboratory diagnosis, and an ... | 2004 | 15671746 |
| [emerging diseases. crimean-congo hemorrhagic fever]. | introduction: recognized for many years in central asia and eastern europe, crimean-congo hemorrhagic fever (cchf) is a severe zoonotic disease which affects people coming into contact with livestock or ticks. the range of the cchf virus is now known to extend form central asia to india, pakistan, afghanistan, iran, iraq, the middle east, eastern europe, and to most of saharan and sub-saharan africa. etiology: cchf virus is a member of the bunyavirus family, and is classified as a nairovirus. cl ... | 2004 | 15675618 |
| bichat guidelines for the clinical management of haemorrhagic fever viruses and bioterrorism-related haemorrhagic fever viruses. | haemorrhagic fever viruses (hfvs) are a diverse group of viruses that cause a clinical disease associated with fever and bleeding disorder. hfvs that are associated with a potential biological threat are ebola and marburg viruses (filoviridae), lassa fever and new world arenaviruses (machupo, junin, guanarito and sabia viruses) (arenaviridae), rift valley fever (bunyaviridae) and yellow fever, omsk haemorrhagic fever, and kyanasur forest disease (flaviviridae). in terms of biological warfare con ... | 2004 | 15677844 |
| [haemorrhagic fever viruses, possible bioterrorist use]. | the majority of haemorrhagic fever viruses are responsible for various clinical manifestations, the mutual characteristics of which are fever and haemorrhage in 5 to 70% of cases. all degrees of severity can be observed, ranging from isolated fever to multi-organ failure and death. these viruses belong to one of the following families: filoviridae, arenaviridae, bunyaviridae, and flaviviridae. they must be considered as dangerous biological weapons that could potentially be used. most of the vir ... | 2005 | 15687968 |
| isolation of bovine arboviruses from culicoides biting midges (diptera: ceratopogonidae) in southern japan: 1985--2002. | in 1985--2002, surveillance for bovine arboviruses was conducted in kagoshima, located in the most southern part of the main islands of japan and known to be an area where bovine arboviral diseases have frequently been epidemic. culicoides biting midges were collected in a cowshed by light traps. a total of 456,300 culicoides biting midges representing 13 species were collected, and a portion of each pool of midges were tested for virus isolation. overall, 85 isolates of six different viruses we ... | 2005 | 15691010 |
| mosquitoborne viruses, czech republic, 2002. | specimens from residents (n = 497) of an area affected by the 2002 flood were examined serologically for mosquitoborne viruses. antibodies were detected against tahyna (16%), sindbis (1%), and batai (0.2%) viruses, but not west nile virus. an examination of paired serum samples showed 1 tahyna bunyavirus (california group) infection. | 2005 | 15705333 |
| loss of milk yield due to akabane disease in dairy cows. | akabane disease is an infection with clinical signs of congenital malformation and abortion in ruminants. abnormal parturitions caused by akabane disease result in great economic loss. the purpose of this study is to estimate the reduction in the milk yield from abnormal parturition due to akabane disease. the data were collected from 33 holstein cows on 11 farms. the animals had abnormal parturitions during the period from september 1998 to march 1999, and were diagnosed as having akabane disea ... | 2005 | 15805732 |
| rift valley fever virus nonstructural protein nss promotes viral rna replication and transcription in a minigenome system. | rift valley fever virus (rvfv), which belongs to the genus phlebovirus, family bunyaviridae, has a tripartite negative-strand genome (s, m, and l segments) and is an important mosquito-borne pathogen for domestic animals and humans. we established an rvfv t7 rna polymerase-driven minigenome system in which t7 rna polymerase from an expression plasmid drove expression of rna transcripts for viral proteins and minigenome rna transcripts carrying a reporter gene between both termini of the m rna se ... | 2005 | 15827175 |
| intracellular localization of crimean-congo hemorrhagic fever (cchf) virus glycoproteins. | crimean-congo hemorrhagic fever virus (cchfv), a member of the genus nairovirus, family bunyaviridae, is a tick-borne pathogen causing severe disease in humans. to better understand the cchfv life cycle and explore potential intervention strategies, we studied the biosynthesis and intracellular targeting of the glycoproteins, which are encoded by the m genome segment. | 2005 | 15850490 |
| attenuation of bunyavirus replication by rearrangement of viral coding and noncoding sequences. | bunyamwera virus (bun) is the prototype virus of the family bunyaviridae. bun has a tripartite negative-sense rna genome comprising small (s), medium (m), and large (l) segments. partially complementary untranslated regions (utrs) flank the coding region of each segment. the terminal 11 nucleotides of these utrs are conserved between the three segments, while the internal regions are unique. the utrs direct replication and transcription of viral rna and are sufficient to allow encapsidation of v ... | 2005 | 15890933 |
| california serogroup gc (g1) glycoprotein is the principal determinant of ph-dependent cell fusion and entry. | members of the california serogroup of orthobunyaviruses, particularly la crosse (lac) and tahyna (tah) viruses, are significant human pathogens in areas where their mosquito vectors are endemic. previous studies using wild-type lac and tah181/57, a highly neurovirulent strain with low neuroinvasiveness (janssen, r., gonzalez-scarano, f., nathanson, n., 1984. mechanisms of bunyavirus virulence. comparative pathogenesis of a virulent strain of la crosse and an avirulent strain of tahyna virus. la ... | 2005 | 15923017 |
| the role of particle-mediated dna vaccines in biodefense preparedness. | particle-mediated epidermal delivery (pmed) of dna vaccines is based on the acceleration of dna-coated gold directly into the cytoplasm and nuclei of living cells of the epidermis, facilitating dna delivery and gene expression. professional antigen-presenting cells and keratinocytes in the skin are both targeted, resulting in antigen presentation via direct transfection and cross-priming mechanisms. only a small number of cells need to be transfected to elicit humoral, cellular and memory respon ... | 2005 | 15935876 |
| detection and localization of rice stripe virus gene products in vivo. | the genome of the tenuivirus, rice stripe virus (rsv) comprises four rnas, the smallest three of which each contain two open reading frames (orfs) arranged in an ambisense manner. the expression of the orfs from rnas 2-4 in plants and the insect vector, laodelphax striatellus, was studied using antisera raised against the gene products. in western blotting of the proteins from infected plants, the molecular masses of p2, p3, pc3 (nucleocapsid protein, n) and p4 (major non-structural protein, ncp ... | 2005 | 16025247 |
| efficient cdna-based rescue of la crosse bunyaviruses expressing or lacking the nonstructural protein nss. | la crosse virus (lacv) belongs to the bunyaviridae family and causes severe encephalitis in children. it has a negative-sense rna genome which consists of the three segments l, m, and s. we successfully rescued lacv by transfection of just three plasmids, using a system which was previously established for bunyamwera virus (lowen et al., virology 330:493-500, 2004). these cdna plasmids represent the three viral rna segments in the antigenomic orientation, transcribed intracellularly by the t7 rn ... | 2005 | 16051834 |
| molecular epidemiology of group c viruses (bunyaviridae, orthobunyavirus) isolated in the americas. | to date, no molecular studies on group c viruses (bunyaviridae, orthobunyavirus) have been published. we determined the complete small rna (srna) segment and partial medium rna segment nucleotide sequences for 13 group c members. the full-length srna sequences ranged from 915 to 926 nucleotides in length, and revealed similar organization in comparison with other orthobunyaviruses. based on the 705 nucleotides of the n gene, group c members were distributed into three major phylogenetic groups, ... | 2005 | 16051848 |
| specific and common changes in nicotiana benthamiana gene expression in response to infection by enveloped viruses. | microarrays derived from solanum tuberosum expressed sequence tags were used to test the hypothesis that genetically distinct enveloped viruses elicit unique changes in nicotiana benthamiana gene expression. the results of our study, which included sonchus yellow net virus (synv), a plant rhabdovirus that replicates in the nucleus of infected cells, and impatiens necrotic spot virus (insv), a plant bunyavirus that replicates in the cytoplasm, were consistent with this hypothesis. statistically s ... | 2005 | 16099921 |
| key golgi factors for structural and functional maturation of bunyamwera virus. | several complex enveloped viruses assemble in the membranes of the secretory pathway, such as the golgi apparatus. among them, bunyaviruses form immature viral particles that change their structure in a trans-golgi-dependent manner. to identify key golgi factors for viral structural maturation, we have purified and characterized the three viral forms assembled in infected cells, two intracellular intermediates and the extracellular mature virion. the first viral form is a pleomorphic structure w ... | 2005 | 16103138 |
| immunogenicity of combination dna vaccines for rift valley fever virus, tick-borne encephalitis virus, hantaan virus, and crimean congo hemorrhagic fever virus. | dna vaccines for rift valley fever virus (rvfv), crimean congo hemorrhagic fever virus (cchfv), tick-borne encephalitis virus (tbev), and hantaan virus (htnv), were tested in mice alone or in various combinations. the bunyavirus vaccines (rvfv, cchfv, and htnv) expressed gn and gc genes, and the flavivirus vaccine (tbev) expressed the prem and e genes. all vaccines were delivered by gene gun. the tbev dna vaccine and the rvfv dna vaccine elicited similar levels of antibodies and protected mice f ... | 2006 | 16174542 |
| mutational analyses of the nonconserved sequences in the bunyamwera orthobunyavirus s segment untranslated regions. | bunyamwera virus (bunv) is the prototype of the genus orthobunyavirus and the family bunyaviridae. bunv has a tripartite genome of negative-sense rna composed of small (s), medium (m), and large (l) segments. partially complementary untranslated regions (utrs) flank the coding region of each segment. the terminal 11 nucleotides of these utrs are conserved between the three segments and throughout the genus, while the internal regions are unique to each segment and largely nonconserved between di ... | 2005 | 16188988 |
| homotypic interaction of bunyamwera virus nucleocapsid protein. | the bunyavirus nucleocapsid protein, n, plays a central role in viral replication in encapsidating the three genomic rna segments to form functional templates for transcription and replication by the viral rna-dependent rna polymerase. here we report functional mapping of interacting domains of the bunyamwera orthobunyavirus n protein by yeast and mammalian two-hybrid systems, immunoprecipitation experiments, and chemical cross-linking studies. n forms a range of multimers from dimers to high-mo ... | 2005 | 16189017 |
| complete l segment coding-region sequences of crimean congo hemorrhagic fever virus strains from the russian federation and tajikistan. | the large (l) rna segment of crimean congo hemorrhagic fever (cchf) virus strain ast/ti30908, isolated from pooled hyalomma marginatum ticks collected in 2002 from the astrakhan region of european russia, was amplified piecemeal using reverse-transcription/polymerase chain reaction, followed by direct sequencing of gel-purified amplicons. after removal of 5' and 3' primer-generated termini, the assembled ast/ti30908 l segment sequence is 12112 nucleotides long, with 41.3% g + c content, and is g ... | 2006 | 16195783 |
| emerging infectious diseases: the bunyaviridae. | the bunyaviridae are a large group of viruses that infect a diversity of arthropod vectors and animal hosts. they have a worldwide distribution and can be the cause of human illness ranging from mild asymptomatic infection to hemorrhagic fever and fatal encephalitis. the growth of the human population, the expansion of agricultural and economic development, climatic changes, and the speed and frequency of global transportation all favor the emergence of bunyaviruses and other arthropod borne vir ... | 2005 | 16287682 |
| oropouche virus isolation, southeast brazil. | an oropouche virus strain was isolated from a novel host (callithrix sp.) in arinos, minas gerais state, southeastern brazil. the virus was identified by complement fixation test and confirmed by reverse transcription-polymerase chain reaction. phylogenetic analysis identified this strain as a genotype iii isolate previously recognized only in panama. | 2005 | 16318707 |
| genetic elements regulating packaging of the bunyamwera orthobunyavirus genome. | the genome of bunyamwera virus (bun; family bunyaviridae, genus orthobunyavirus) comprises three segments of negative-sense, single-stranded rna. the rna segments are encapsidated by the viral nucleocapsid (n) protein and form panhandle-like structures through interaction of complementary sequences at their 5' and 3' termini. transcription and replication of a bun genome analogue (minireplicon), comprising the viral non-coding sequences flanking a reporter gene, requires just the viral rna polym ... | 2006 | 16361430 |
| identification of the bunyamwera bunyavirus transcription termination signal. | bunyamwera virus (bunv) is the prototype of the family bunyaviridae, which comprises segmented rna viruses. each of the bunv negative-strand segments, small (s), medium (m) and large (l), serves as template for two distinct rna-synthesis activities: (i) replication to generate antigenomes that are in turn replicated to yield further genomes; and (ii) transcription to generate a single species of mrna. bunv mrnas are truncated at their 3' ends relative to the genome template, presumably because t ... | 2006 | 16361431 |
| isolations of potosi virus from mosquitoes (diptera: culicidae) collected in connecticut. | potosi virus (potv) (bunyaviridae: orthobunyavirus) was first isolated from aedes albopictus (skuse) collected in potosi, mo, in 1989, and subsequent isolations were reported from illinois, michigan, ohio, and the carolinas. to determine whether the distribution of this virus extends into the northeastern united states, we analyzed arboviruses acquired from mosquitoes collected in connecticut from 1998 to 2004. in 2001, a bunyavirus was isolated from aedes vexans (meigen) that was different from ... | 2005 | 16363172 |
| isolation of viruses from mosquitoes (diptera: culicidae) collected in the amazon basin region of peru. | as part of a comprehensive study on the ecology of arthropod-borne viruses in the amazon basin region of peru, we assayed 539,694 mosquitoes captured in loreto department, peru, for arboviruses. mosquitoes were captured either by dry ice-baited miniature light traps or with aspirators while mosquitoes were landing on human collectors, identified to species, and later tested on vero cells for virus. in total, 164 virus isolations were made and included members of the alphavirus (eastern equine en ... | 2005 | 16366001 |
| rift valley fever virus. | rift valley fever is considered to be one of the most important viral zoonoses in africa. in 2000, the rift valley fever virus spread to the arabian peninsula and caused two simultaneous outbreaks in yemen and saudi arabia. it is transmitted to ruminants and to humans by mosquitoes. the viral agent is an arbovirus, which belongs to the phlebovirus genus in the bunyaviridae family. this family of viruses comprises more than 300 members grouped into five genera: orthobunyavirus, phlebovirus, hanta ... | 2005 | 16375716 |
| assay and functional analysis of dynamin-like mx proteins. | mx proteins are interferon-induced large guanosine triphosphatases (gtpases) that share structural and functional properties with dynamin and dynamin-like proteins, such as self-assembly and association with intracellular membranes. a unique property of some mx proteins is their antiviral activity against a range of rna viruses, including influenza viruses and members of the bunyavirus family. these viruses are inhibited at an early stage in their life cycle, soon after host cell entry and befor ... | 2005 | 16413306 |
| the bunyavirus nucleocapsid protein is an rna chaperone: possible roles in viral rna panhandle formation and genome replication. | cellular rna chaperones are crucial for the genesis of correctly folded functional rnas. using several complementary in vitro assays we find that the bunyavirus nucleocapsid protein (n) is an rna chaperone. in the bunyaviridae genomic rna is in stable "panhandle" formation that arises through the hydrogen bonding of the terminal nucleotides of the rna. the rna chaperone function of n facilitates panhandle formation even though the termini are separated by >2 kb. rna panhandle formation is likely ... | 2006 | 16428606 |
| characterization of maguari orthobunyavirus mutants suggests the nonstructural protein nsm is not essential for growth in tissue culture. | maguari virus (magv; genus orthobunyavirus, family bunyaviridae) contains a tripartite negative-sense rna genome. like all orthobunyaviruses, the medium (m) genome segment encodes a precursor polyprotein (nh(2)-gn-nsm-gc-cooh) for the two virion glycoproteins gn and gc and a nonstructural protein nsm. the nucleotide sequences of the m segment of wild-type (wt) magv, of a temperature-sensitive (ts) mutant, and of two non-ts revertants, r1 and r2, that show electrophoretic mobility differences in ... | 2006 | 16445958 |
| quantitative analysis of la crosse virus transcription and replication in cell cultures and mosquitoes. | la crosse (lac) virus (family bunyaviridae, genus orthobunyavirus) small (s) segment negative-sense rna genome (vrna), positive-sense full-length rna complement (vcrna), and subgenomic mrna were assayed in infected cell cultures and female aedes (ochlerotatus) triseriatus mosquito tissues using quantitative pcr (q-pcr). during persistent infection of c6/36 (aedes albopictus) and mat (aedes triseriatus) cultured cells and cytolytic infection of bhk-21 cultured cells, lac vrna was the most abundan ... | 2006 | 16474075 |
| rescue of infectious rift valley fever virus entirely from cdna, analysis of virus lacking the nss gene, and expression of a foreign gene. | rift valley fever virus (rvfv) (genus phlebovirus, family bunyaviridae) has a tripartite negative-strand genome, causes a mosquito-borne disease that is endemic in sub-saharan african countries and that also causes large epidemics among humans and livestock. furthermore, it is a bioterrorist threat and poses a risk for introduction to other areas. in spite of its danger, neither veterinary nor human vaccines are available. we established a t7 rna polymerase-driven reverse genetics system to resc ... | 2006 | 16501102 |
| seroprevalence of tick-borne and mosquito-borne arboviruses in european brown hares in northern and western germany. | tríbec virus (kemerovo serogroup, genus orbivirus), eyach virus (genus coltivirus), and tahyna virus (california encephalitis serogroup, genus bunyavirus) are arthropod-borne viruses known to occur in germany. these viruses are also suspected to cause human disease. so far, no information is available on their geographical distribution in germany and their natural transmission cycles. a total of 166 sera from european brown hares (lepus europaeus) collected in seven districts of the federal stat ... | 2006 | 16530475 |
| batai and ngari viruses: m segment reassortment and association with severe febrile disease outbreaks in east africa. | ngari virus is an orthobunyavirus recently recognized as a reassortant between bunyamwera virus and an as yet unidentified m segment donor. analysis of m segment sequences of batai and ilesha viruses revealed 95% deduced amino acid identity between batai virus and ngari virus. these findings suggest batai virus as the donor of ngari virus m segment sequence. analysis of batai virus-related african isolates identified ugmp-6830, isolated from mosquitoes in uganda, as an isolate of batai virus. kv ... | 2006 | 16699043 |
| chronological and geographical variations in the small rna segment of the teratogenic akabane virus. | characterization of the small rna segment of the teratogenic akabane virus was performed to define the genetic heterogeneity and molecular epidemiology of the virus. the nucleotide and amino acid sequences were highly conserved among the akabane virus isolates, irrespective of the year of isolation and geographical origin, except for a kenyan strain mp496. on the basis of the nucleocapsid gene sequences of the segment, 170 field isolates were grouped into four distinct phylogenetic lineages. the ... | 2006 | 16730837 |
| survey of aedes triseriatus (diptera: culicidae) for lacrosse encephalitis virus and west nile virus in lorain county, ohio. | from june through september 2003, we conducted a survey of female aedes triseriatus (say) for infection with la crosse encephalitis virus (family bunyaviridae, genus orthobunyavirus, lacv) and west nile virus (family flaviviridae, genus flavivirus, wnv) at three locations in lorain county, ohio. to determine infection rate and seasonal variation of both viruses in the ae. triseriatus population, ae. triseriatus were collected weekly by using gravid traps and co2-baited cdc light traps and tested ... | 2006 | 16739420 |
| the acre project: the epidemiology of malaria and arthropod-borne virus infections in a rural amazonian population. | the authors describe the baseline malaria prevalence and arbovirus seroprevalence among 467 subjects in an ongoing cohort study in rural amazonia. most subjects (72.2%) reported one or more previous episodes of malaria, and 15.6% had been hospitalized for malaria, but only 3.6% of individuals five years or older had malaria parasites detected by microscopy (10 with plasmodium vivax and 4 with p. falciparum). antibodies to alphavirus, orthobunyavirus, and/or flavivirus were detected by hemaggluti ... | 2006 | 16751971 |
| [west nile virus and its vectors]. | there are more than five hundred known arthropod-borne viruses (arboviruses) all around the world and approximately hundred of them may cause disease in humans. during the past 20 years there has been a dramatic resurgence or emergence of epidemic arboviral diseases affecting both humans and domestic animals. many factors play important roles in the emergence of arboviral diseases like yellow fever, dengue, west nile encephalitis, and of other diseases such as malaria and leishmaniasis in countr ... | 2006 | 16775967 |
| studying cellular architecture in three dimensions with improved resolution: ta replicas revisited. | metal replicas have been used for surface analysis of biological structures with a variety of spatial resolutions. platinum (pt) has been the metal of choice because it provides very stable replicas and images of high contrast. some other metals, such as tantalum (ta) have been reported to provide better resolution on isolated macromolecular complexes and cellular structures. our goal is to study the gain in detail with ta and to evaluate if it provides enough detail and resolution to assist in ... | 2006 | 16798024 |
| genetic characterization of batai virus indicates a genomic reassortment between orthobunyaviruses in nature. | two viruses were isolated from bovine blood in the southernmost part of japan in 1994 and 2001, respectively. genetic analyses showed that the viruses were batai virus of the genus orthobunyavirus of the family bunyaviridae. the sequencing of three genomic rna segments of the japanese and malaysian batai virus strains revealed that the m rna segment of batai virus had high sequence identity with that of ngari virus. our results indicate that ngari virus is a genetic reassortant with s and l rna ... | 2006 | 16820982 |
| requirement of the n-terminal region of orthobunyavirus nonstructural protein nsm for virus assembly and morphogenesis. | the nonstructural protein nsm of bunyamwera virus (bunv), the prototype of the bunyaviridae family, is encoded by the m segment in a polyprotein precursor, along with the virion glycoproteins, in the order gn-nsm-gc. as little is known of its function, we examined the intracellular localization, membrane integrality, and topology of nsm and its role in virus replication. we confirmed that nsm is an integral membrane protein and that it localizes in the golgi complex, together with gn and gc. coi ... | 2006 | 16873265 |
| a new genetic variant of la crosse virus (bunyaviridae) isolated from new england. | la crosse virus (lacv) is found primarily in the midwestern and appalachian regions of the united states where it is a leading cause of mosquito-borne encephalitis in children. to determine whether the distribution of this virus extends further east into new england, we analyzed a bunyavirus that was isolated from a pool of eastern tree-hole mosquitoes, ochlerotatus triseriatus (= aedes triseriatus), collected from fairfield, connecticut (ct) in 2005. nucleotide and encoded amino acid sequences ... | 2006 | 16968927 |
| hantavirus n protein exhibits genus-specific recognition of the viral rna panhandle. | a key genomic characteristic that helps define hantavirus as a genus of the family bunyaviridae is the presence of distinctive terminal complementary nucleotides that promote the folding of the viral genomic segments into "panhandle" hairpin structures. the hantavirus nucleocapsid protein (n protein), which is encoded by the smallest of the three negative-sense genomic rna segments, undergoes in vivo and in vitro trimerization. trimeric hantavirus n protein specifically recognizes the panhandle ... | 2006 | 16971445 |
| interaction of bunyamwera orthobunyavirus nss protein with mediator protein med8: a mechanism for inhibiting the interferon response. | the nss protein of bunyamwera virus (bunyaviridae) is an antiapoptotic interferon antagonist involved in silencing host protein expression by interfering with mrna synthesis. here, we show that the ability to inhibit both host transcription and the interferon response is linked to interaction of nss with the med8 component of mediator, a protein complex necessary for mrna production. the interacting domain on nss was mapped to the c-terminal region, which contains amino acids conserved among ort ... | 2006 | 16973571 |
| isolation of bunyamwera serogroup viruses (bunyaviridae, orthobunyavirus) in new york state. | during routine arbovirus surveillance from 2000 to 2004 in new york state (nys), 14,788 mosquito pools making up 36 species and nine genera were inoculated onto vero cell cultures to test for abroad spectrum of viruses. forty-six percent of viruses isolated in cell culture from species, excluding culex pipiens l. and culex restuans theobald, were identified as bunyamwera serogroup viruses. here, we report the distribution and level of bunyamwera activity in nys detected during this period. we de ... | 2006 | 17017240 |
| in vitro and in vivo studies of ribavirin action on brazilian orthobunyavirus. | oropouche, caraparu, guama, guaroa, and tacaiuma are viruses (genus orthobunyavirus) that cause human febrile illnesses and encephalitis. the goal of this study was to evaluate the antiviral action of ribavirin on these orthobunyaviruses to achieve a therapeutical agent to treat the diseases caused by these viruses. in vitro results showed that ribavirin (50 microg/ml) had antiviral activity only on the tacaiuma virus. addition of guanosine in the culture reversed the antiviral effect of ribavir ... | 2006 | 17124004 |
| descriptive and spatial epidemiology of rift valley fever outbreak in yemen 2000-2001. | rift valley fever (rvf) is an arboviral disease produced by a bunyavirus belonging to the genus phlebovirus. several species of aedes and culex are the vectors of this virus that affects sheep, goats, buffalos, cattle, camels and human beings. the human disease is well known, especially during periods of intense epizootic activity. the initial description of the disease dates back to 1930, when animals and human outbreaks appeared on a farm in lake naivasha, in the great rift valley of kenya. un ... | 2006 | 17135517 |
| antiviral activity and rna polymerase degradation following hsp90 inhibition in a range of negative strand viruses. | we have analyzed the effectiveness of hsp90 inhibitors in blocking the replication of negative-strand rna viruses. in cells infected with the prototype negative strand virus vesicular stomatitis virus (vsv), inhibiting hsp90 activity reduced viral replication in cells infected at both high and low multiplicities of infection. this inhibition was observed using two hsp90 inhibitors geldanamycin and radicicol. silencing of hsp90 expression using sirna also reduced viral replication. hsp90 inhibiti ... | 2007 | 17258257 |
| in vitro study of antiviral activity of mycophenolic acid on brazilian orthobunyaviruses. | oropouche, caraparu, guama, guaroa and tacaiuma are ssrna viruses that belong to the genus orthobunyavirus and have been associated with human febrile illnesses and/or encephalitis. in this study, we evaluated the antiviral action of mycophenolic acid (mpa) on theseorthobunyaviruses to achieve a therapeutic agent to treat the diseases caused by these viruses. | 2007 | 17283446 |
| la crosse bunyavirus nonstructural protein nss serves to suppress the type i interferon system of mammalian hosts. | la crosse virus (lacv) is a mosquito-transmitted member of the bunyaviridae family that causes severe encephalitis in children. for the lacv nonstructural protein nss, previous overexpression studies with mammalian cells had suggested two different functions, namely induction of apoptosis and inhibition of rna interference (rnai). here, we demonstrate that mosquito cells persistently infected with lacv do not undergo apoptosis and mount a specific rnai response. recombinant viruses that either e ... | 2007 | 17344298 |
| in vitro and in vivo studies of the interferon-alpha action on distinct orthobunyavirus. | oropouche, caraparu, guama, guaroa and tacaiuma viruses (orthobunyavirus genus) cause human febrile illnesses and/or encephalitis. to achieve a therapeutical agent to prevent and/or treat these diseases we evaluated the antiviral action of interferon-alpha (ifn-alpha) on these orthobunyaviruses. in vitro results showed that all the studied orthobunyaviruses are susceptible to antiviral action of ifn-alpha, but this susceptibility is limited and dependent on both concentration of drug and treatme ... | 2007 | 17368573 |
| a novel, multipartite, negative-strand rna virus is associated with the ringspot disease of european mountain ash (sorbus aucuparia l.). | four rnas from a new plant-pathogenic virus, which we have tentatively named european mountain ash ringspot-associated virus (emarav), were identified and sequenced completely. all four viral rnas could be detected in previous double-stranded rna preparations. rna 1 (7040 nt) encodes a protein with similarity to the rna-dependent rna polymerase of different members of the bunyaviridae, a family containing five genera with viruses infecting invertebrates, vertebrates and plants. rna 2 (2335 nt) e ... | 2007 | 17374780 |
| bunyavirus mrna synthesis is coupled to translation to prevent premature transcription termination. | messenger rna transcription by bunyaviridae family members is unique within the group of negative-strand rna viruses as it requires on-going protein synthesis. the long-standing model explaining this phenomenon proposes that the translational requirement is not for a protein product, but instead is for ribosomal translocation along nascent mrnas. this movement is proposed to disrupt spurious transcription termination signals that otherwise cause premature mrna truncation leading to a fatal loss ... | 2007 | 17400818 |
| identification of a novel c-terminal cleavage of crimean-congo hemorrhagic fever virus pregn that leads to generation of an nsm protein. | the structural glycoproteins of crimean-congo hemorrhagic fever virus (cchfv; genus nairovirus, family bunyaviridae) are derived through endoproteolytic cleavage of a 1,684-amino-acid m rna segment-encoded polyprotein. this polyprotein is cotranslationally cleaved into the pregn and pregc precursors, which are then cleaved by ski-1 and a ski-1-like protease to generate the n termini of gn and gc, respectively. however, the resulting polypeptide defined by the n termini of gn and gc is predicted ... | 2007 | 17409136 |
| comparison of akabane virus isolated from sentinel cattle in japan. | adult cows, ewes, and goats infected with akabane virus (akav) of the genus orthobunyavirus of the family bunyaviridae do not present any clinical signs; however, in utero infections may result in abortion, premature birth, stillbirth, and congenital deformities such as arthrogryposis-hydranencephaly syndrome in cattle, sheep, and goats. in contrast, the iriki strain, a variant of akav isolated from a calf with nervous signs and encephalitis, causes encephalitis in experimentally inoculated calv ... | 2007 | 17467929 |
| molecular characterization of african orthobunyaviruses. | the genus orthobunyavirus is composed of segmented, negative-sense rna viruses that are responsible for mild to severe human diseases. to date, no molecular studies of bunyaviruses in the genus orthobunyavirus from central africa have been reported, and their classification relies on serological testing. four new primer pairs for rt-pcr amplification and sequencing of the complete genomic small (s) rna segments of 10 orthobunyaviruses isolated from the central african republic and pertaining to ... | 2007 | 17485537 |
| characterization of temperature-sensitive akabane virus mutants and their roles in attenuation. | akabane virus (akav) of the genus orthobunyavirus in the family bunyaviridae is an important animal pathogen; however, studies on akav biology are scarce. therefore, we generated temperature-sensitive (ts) mutants of akav in order to study its pathogenesis. the ts akav mutants were generated by incubating the virulent obe-1 strain with the chemical mutagen 5-fluorouracil. each ts mutant was inoculated intracerebrally into mice to assess its virulence, and the genomic sequences of the attenuated ... | 2007 | 17533552 |
| emergent arboviruses in brazil. | brazil is a large tropical country (8,514,215 km(2)) with 185,360,000 inhabitants. more than one third of its territory is covered by tropical forests or other natural ecosystems. these provide ideal conditions for the existence of many arboviruses, which are maintained in a large variety of zoonotic cycles. the risk that new arboviruses might emerge in brazil is related to the existence of large, densely populated cities that are infested by mosquitoes such as culex and the highly anthropophili ... | 2007 | 17568894 |
| interferon-induced mx proteins in antiviral host defense. | mx proteins are key components of the antiviral state induced by interferons in many species. they belong to the class of dynamin-like large guanosine triphosphatases (gtpases) known to be involved in intracellular vesicle trafficking and organelle homeostasis. mx gtpases share structural and functional properties with dynamin, such as self-assembly and association with intracellular membranes. a unique property of some mx gtpases is their antiviral activity against a wide range of rna viruses, ... | 2007 | 17570575 |
| in vitro and in vivo activities of t-705 against arenavirus and bunyavirus infections. | there is a need for the development of effective antivirals for the treatment of severe viral diseases caused by members of the virus families bunyaviridae and arenaviridae. the pyrazine derivative t-705 (6-fluoro-3-hydroxy-2-pyrazinecarboxamide) has demonstrated remarkable antiviral activity against influenza virus and, to a lesser degree, against some other rna viruses (y. furuta, k. takahashi, y. fukuda, m. kuno, t. kamiyama, k. kozaki, n. nomura, h. egawa, s. minami, y. watanabe, h. narita, ... | 2007 | 17606691 |
| role of the cytoplasmic tail domains of bunyamwera orthobunyavirus glycoproteins gn and gc in virus assembly and morphogenesis. | the m rna genome segment of bunyamwera virus (bunv), the prototype of the bunyaviridae family, encodes a precursor polyprotein that is proteolytically cleaved to yield two structural proteins, gn and gc, and a nonstructural protein called nsm. gn and gc are type i integral transmembrane glycoproteins. the gn protein contains a predicted cytoplasmic tail (ct) of 78 residues, and gc has a shorter ct of 25 residues. little is known about the role of the gn and gc ct domains in the virus replication ... | 2007 | 17609275 |
| validation of an indirect elisa based on a recombinant nucleocapsid protein of rift valley fever virus for the detection of igg antibody in humans. | an indirect enzyme-linked immunoassay (i-elisa) based on the recombinant nucleocapsid protein (rnp) of rift valley fever virus was validated for the detection of specific igg antibody in human sera. validation data sets derived from testing sera collected in africa (n=2967) were categorized according to the results of a virus neutralisation test. the assay had high intra- and inter-plate repeatability in routine runs. no detectable cross-reactions between igg antibodies generated from mice exper ... | 2007 | 17645952 |
| genetic diversity and reassortments among akabane virus field isolates. | sequencing and phylogenetic analysis were carried out for 35 akabane virus (akav) field isolates collected from japan, taiwan, australia and kenya, and for one tinaroo virus (tinv). of the three rna segments, the m rna segment encoding the glycoproteins that induce neutralization antibodies was the most variable among the isolates. the difference in the m rna segments among asian (japanese and taiwanese) isolates was not large (<12.3% nucleotide (nt) and <5.9% amino acid (aa) differences), rathe ... | 2007 | 17659802 |
| the cytoplasmic tails of uukuniemi virus (bunyaviridae) g(n) and g(c) glycoproteins are important for intracellular targeting and the budding of virus-like particles. | functional motifs within the cytoplasmic tails of the two glycoproteins g(n) and g(c) of uukuniemi virus (uuk) (bunyaviridae family) were identified with the help of our recently developed virus-like particle (vlp) system for uuk virus (a. k. overby, v. popov, e. p. neve, and r. f. pettersson, j. virol. 80:10428-10435, 2006). we previously reported that information necessary for the packaging of ribonucleoproteins into vlps is located within the g(n) cytoplasmic tail (a. k. overby, r. f. petters ... | 2007 | 17670814 |
| recent progress in molecular biology of crimean-congo hemorrhagic fever. | crimean-congo hemorrhagic fever (cchf) is a severe hemorrhagic fever in humans with a case fatality rate of up to 50%. a causative agent of cchf is cchf virus, which is a tick-borne virus in the family bunyaviridae, genus nairovirus. the virus is transmitted to humans through infected tick bites, squashed ticks or from direct contact with viremic animals or humans. outbreaks of cchf have been documented in africa, the middle east, eastern europe and western asia where the vector and/or reservoir ... | 2007 | 17692916 |
| caraparu virus (group c orthobunyavirus): sequencing and phylogenetic analysis based on the conserved region 3 of the rna polymerase gene. | here, for the first time, we report the nucleotide sequence of caraparu virus (carv) l segment and the analysis of the rna polymerase region 3 encoded by this segment. the 1,404 bp nucleotide sequence shares the highest identity with bunyamwera, la crosse, oropouche, and akabane virus sequences. the amino acid sequence was deduced and aligned with sequences from members of the bunyaviridae family and used for phylogenetic analysis. the carv clustered in the orthobunyavirus genus. the premotif a ... | 2007 | 17705031 |
| [brazil, land of choice for arbovirosis?]. | scores of arboviroses have been indexed in brazil with variable rates of morbidity and mortality. emergence of arboviroses and their transmission by hematophageous vectors are closely related to the country's recent history and in particular to its economic, social and ecological development. most arboviroses are caused by alphaviruses and flaviviruses. some species of bunyavirus are also found. brazil is often regarded as an arbovirus sanctuary where dormant or nonpathogenic viruses can find an ... | 2007 | 17784683 |
| natural m-segment reassortment in potosi and main drain viruses: implications for the evolution of orthobunyaviruses. | recently, we identified batai virus as the m-segment reassortment partner of ngari virus. extension of genetic analyses to other orthobunyaviruses related to the bunyamwera serogroup indicates additional natural genome reassortments. whereas the relative phylogenetic positions of all three genome segment sequences were similar for northway and kairi viruses, the relative positions of potosi and main drain virus m-segment sequences diverged from those of their s- and l-segments. our findings indi ... | 2007 | 17891328 |
| rna binding domain of jamestown canyon virus s segment rnas. | jamestown canyon virus (jcv) is a member of the bunyaviridae family, orthobunyavirus genus, california serogroup. replication and, ultimately, assembly and packaging rely on the process of encapsidation. therefore, the ability of viral rnas (vrnas) (genomic and antigenomic) to interact with the nucleocapsid protein (n protein) and the location of this binding domain on the rnas are of interest. the questions to be addressed are the following. where is the binding domain located on both the vrna ... | 2007 | 17913826 |
| application of modified shell vial culture procedure for arbovirus detection. | the isolation of arboviruses from patient's low titer sera can be difficult. here we compared the detection efficiency of dengue (den), yellow fever (yf), saint louis encephalitis (sle), west nile (wn), ilheus (ilh), group c (gc), oropouche (oro), mayaro (may) and venezuela encephalitis equine (vee) viruses using a modified shell vial culture (msvc) protocol to a standard cell culture (scc) protocol. first the msvc and scc protocols were compared using five dilutions for each of the following st ... | 2007 | 17940598 |
| prevalence and protein specificity of human antibodies to inkoo virus infection. | inkoo virus (inkv), a member of the california serogroup orthobunyaviruses, is circulating widely in northern europe. although the virus was discovered over 40 years ago, the disease associations and immune responses in human infection are poorly characterized. we first developed an immunofluorescence assay (ifa) for the detection of inkv antibodies in humans, and then we studied a panel of 1,292 sera in patients with a febrile illness in finland. we found four acute (immunoglobulin m [igm] posi ... | 2007 | 17942611 |