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[comparative study of the hemolytic activity of ortho- and paramyxoviruses].a comparative study of hemolytic activity of influenza type a, b, and c viruses, human parainfluenza type 3, and sendai virus showed the pattern of ph-dependence and the nature of the curve to differ not only for different viruses under study but also for different erythrocyte species. studies of virus-induced hemolysis of influenza c virus demonstrated that, depending on the erythrocyte species used, it had common properties both with influenza types a and b viruses and with paramyxoviruses.19892548343
[hla system antigens in persons with differing susceptibility to the causative agents of acute respiratory diseases].the relationship between the susceptibility of the body to infections caused by influenza a and b viruses, parainfluenza viruses, adenoviruses, mycoplasma pneumoniae and antigens of the hla system was studied on a group of 400 adolescents placed under clinico-epidemiological surveillance for two years. the relationship between histocompatibility antigens and acute respiratory diseases was manifested in a decrease or increase in the occurrence of recurrent diseases and infections or in the probab ...19892558471
matrix proteins of influenza a and b viruses possess common antigenic determinants. 19852578594
antigenic analysis of influenza b virus isolated from the epidemic in 1973. 19852581136
[matrix protein of the influenza virus: properties, functions and role in the diagnosis of influenza]. 19852581367
leukocyte tubuloreticular inclusions in reye's syndrome.tubuloreticular inclusions (tri) have been observed in the rough endoplasmic reticulum of blood lymphocytes and monocytes in two cases of reye's syndrome initiated by influenza infections. tubuloreticular inclusions are seen in these mononuclear leukocytes during the acute phase of illness, but not during convalescence. since tri have been demonstrated in peripheral mononuclear leukocytes in patients with acquired immunodeficiency syndrome, systemic lupus erythematosus, and certain viral infecti ...19852581524
[spread of influenza viruses among synanthropic and semisynanthropic birds of the middle dnieper from the data of serologic studies]. 19892588558
[neurosonography of the brain in infectious toxicoses in infants].brain neurosonography was performed in 15 babies with toxicosis-+-linked nervous disorders. three types of the brain structure were established in the patients: 1, marked brain swelling in all patients; 2, concomitant perinatally induced brain structures' changes; 3, intracranial hemorrhage focuses resulting from the dic syndrome. the technique is useful for precise diagnosis and prognosis of the outcome of the neurotoxic coma.19892588907
influenza b, q fever, and the consequences of febrile illness occurring during jungle warfare training: a clinical and serological study.seventy-one royal marines undergoing jungle warfare training were studied clinically and serologically to determine the incidence and consequences of febrile illness. during acclimatization and the early part of the deployment the incidence of illness having an impact on training was low; during the latter part an outbreak of influenza b occurred, with 25 cases diagnosed clinically. only 12 of these were confirmed serologically, probably because the outbreak was still in progress when the second ...19892600890
synthesis and antiviral evaluation of 6-p-ethylphenyl-5h-pyrrolo [3,4-b]pyridine-5,7-dione.the synthesis of 6-(p-ethylphenyl)-5h-pyrrolo [3,4-b]pyridine-5,7-dione is described. the compound has been studied for its in vitro activity on rna-containing influenza viruses types a and b. the preliminary results indicate that the compound has a significant antiviral activity against influenza viruses types a and b at concentrations at which no cytotoxic effects on mrc-5 cells in tissue culture are present.19892604840
[antigenic characteristics of influenza b virus strains isolated in an orphanage during an influenza outbreak in moscow in the winter of 1988].examinations of ard patients in an orphanage for defective children in moscow during an influenza outbreak in the winter of 1988 yielded 12 influenza virus strains, including 6 influenza b strains and 6 influenza a (h3n2) strains. the antigenic analysis of hemagglutinin of influenza b virus isolates showed that with respect to the b/leningrad/179/86 strain (an antigenic analogue of b/ann arbor/1/86 strain recommended for inclusion into the influenza vaccine for 1987-1988) they could be divided i ...19892609641
[attenuated recombinant influenza type b virus obtained during crossing of virus b/ann arbor/2/86 with the cold-adapted strain b/leningrad/14/17/55].crossing the cold-adapted b/leningrad/14/17/55 strain with the temperature-sensitive virulent b/ann arbor/2/86 strain yielded a recombinant b/14/5/1 which, by the antigenic specificity of hemagglutinin and neuraminidase, corresponded to the b/ann arbor/2/86 strain but, like the attenuated donor, had the cold-adapter characteristics. the b/14/5/1 recombinant inherited the genes coding for proteins pb2, pb1, pa, np, and m from the attenuated master strain and the genes coding for hemagglutinin, ne ...19892609642
[isolation and crystallization of neuraminidase of the influenza b virus strain b/leningrad/179/86]. 19892609649
[synthesis of virus-specific rna in cells infected with influenza b virus].synthesis of virus-specific rna in cells infected with influenza b virus was studied by means of page analysis of rna hybrid duplexes or nucleocapsid-associated rna. a switch from an "early" to "late" pattern was registered in the relative rates of synthesis of the corresponding mrna as well as in the synthesis of vrna segments 7 and 8. contrary to the pattern described earlier for influenza a virus, the relative rate of synthesis of mrna 5 was increased at the late stage of the replication cycl ...19892615771
an outbreak of influenza in jamaica (1986).an outbreak of febrile illness at the police training academy in st. catherine, jamaica in october, 1986 is described. influenza a similar to type a/taiwan/86 (h1n1) was confirmed serologically in 33 cases, and influenza b similar to type b/ann arbor/86 in 1 case. clinical findings included upper respiratory symptoms (97%) headache (74%), fever (68%), joint pains (39%) and retro-orbital pain (26%). the role of vaccination and chemoprophylaxis in the prevention of influenza is discussed, and the ...19892618029
[the determination of the interdependence of the infectivity of different groups of birds with the influenza virus by using mathematical modelling].when studying sera in the hemagglutination-inhibition reaction which has been taken from 772 fowls of 82 species caught in certain regions of the dnieper in 1981-1987 the antibodies to 29 strains of the influenza virus are revealed, all of them being of human and animal origin. the serological examination has shown the circulation of the influenza virus with hemagglutinin h13 in a wide range of water fowls, the level of antibodies to it being dependent on the species of fowls and season. using t ...19892622402
one-incubation time-resolved fluoroimmunoassay based on monoclonal antibodies in detection of influenza a and b viruses directly in clinical specimens.a new modified enzyme immunoassay screening method was developed for testing hybridoma cultures, so as to select antibodies useful for solid phase assays. samples of hybridoma cultures were incubated for 1 h with purified nucleoprotein preparation in microtiter wells precoated with rabbit anti-influenza a or b immunoglobulin, followed by washing and addition of anti-mouse hrpo-conjugate. the monoclonal antibodies were then used in one-incubation time-resolved fluoroimmunoassay (tr-fia) for detec ...19892643611
immunoassay for serologic diagnosis of influenza type a using recombinant dna produced nucleoprotein antigen and monoclonal antibody to human igg.influenza type a nucleoprotein (np) derived from the full length cloned gene expressed in e. coli was evaluated in a solid phase enzyme immunoassay (eia) for detection of human antibody to influenza. monoclonal antibody to human igg was used for detection. direct and indirect assays were developed and sera were tested in serial and single dilution formats. preliminary results indicated that recombinant-and virion-derived np antigens were comparable in binding ability to plastic and binding human ...19892646393
postinfluenza toxic shock syndrome.postinfluenza toxic shock syndrome is a recently described entity that results from a respiratory tract infection with toxin-producing staphylococcus aureus following an episode of influenza or influenzalike illness. this report describes a 19-year-old man who developed an influenza b respiratory infection complicated by staphylococcal pneumonia and toxic shock syndrome. the patient improved rapidly with specific antibiotic therapy, emphasizing the importance of considering this otherwise highly ...19892651040
rapid detection of influenza virus infections in human fetal lung diploid cell cultures.haemadsorbing foci were found in human fetal lung (hfl) diploid cell cultures 12 h after inoculation with influenza viruses a and b. the size and number of the foci were maximal after 48 h of incubation, being limited by production of an unidentified inhibitor. by contrast, inoculation with parainfluenza virus type 3 led to haemadsorption which increased during 10 days of incubation. for the detection of influenza viruses a and b maximum sensitivity was achieved by changing the medium, the day b ...19892663998
influenza b in transplant patients.six cases of influenza b occurred in transplanted patients in a period of 3 weeks. three renal allograft recipients recovered within 5 days without antiviral therapy. two allogeneic bone marrow recipients were treated with ribavirin inhalations during the leukopenic phase. treatment was given until influenza b was no longer detected and fever disappeared after 5 and 6 days, respectively. engraftment was not delayed and no side-effects were noted. one recipient of autologous marrow was treated fo ...19892667101
rapid 24-well plate centrifugation assay for detection of influenza a virus in clinical specimens.two methods for detection of influenza virus in 234 clinical respiratory specimens were compared: (i) a 24-well plate-centrifugation assay using madin darby canine kidney (mdck) cells and staining with monoclonal antibody pools to influenza a and b (centers for disease control, atlanta, ga) after incubation for 16 h and 40 h, and (ii) conventional tube cell culture using mdck cells and primary rhesus monkey kidney cells. influenza a was identified in 23 specimens (10%). no influenza b was recove ...19892668316
successful use of shell vial centrifugation and 16 to 18-hour immunofluorescent staining for the detection of influenza a and b in clinical specimens.the rapid diagnosis of influenza a and b infections is beneficial for the proper management of patients with acute respiratory illness. the authors evaluated a shell vial centrifugation method to detect these viruses 16-18 hours postinoculation and compared it with conventional tube cell culture. rhesus monkey kidney cells were used in both methods. conventional culture of 334 respiratory specimens recovered 64 influenza isolates; the average time to positivity was 4.1 days. low-speed shell vial ...19892679041
[stat-diagnosis of influenza using lanthanide immunofluorescence analysis].a test system for influenza diagnosis has been developed on the basis of monoclonal antibodies to influenza a and b virus proteins using the principles of lanthanide immunofluorescence analysis. the diagnostic test system was shown to be highly specific in detecting influenza a and b virions in model systems. for the one-step variant of a double sandwich used in the study, the total time of diagnostic experiment using clinical materials was shown to be reduced to 15-20 min.19892692301
influenza serosurvey for 1988-1989 season. 19892702698
natural or vaccine-induced antibody as a predictor of immunity in the face of natural challenge with influenza viruses.a study of influenza in residential schools provided the opportunity to assess the significance of antibody as a predictor of immunity. five hundred and fifty-six pupils from 8 schools were included in the investigations, and the outcome for these children in 27 naturally occurring outbreaks of influenza was analysed. the outbreaks comprised 5 caused by strains of influenza a h3n2, 10 caused by strains of influenza a h1n1, and 12 caused by strains of influenza b. on 8 occasions a second outbreak ...19892703026
improved colorimetric assay for detecting influenza b virus neutralizing antibody responses to vaccination and infection.an automated neutralization test for influenza b virus is described in which antibody titers are determined according to the release of neutral red from infected or uninfected cells of the madin-darby canine kidney line. endpoints are determined in a standard enzyme-linked immunosorbent assay reader. the test requires no expensive immunologic reagents and was used to evaluate responses to both vaccination and natural infection against influenza b virus. overall responses to vaccination were comp ...19892715324
family functioning and stress as predictors of influenza b infection.a prospective cohort study was designed to study the effects of family functioning and stress on the incidence of influenza infection. families from the clinic roster, containing two adults and at least one child between the ages of 1 and 18 years, were asked to participate. baseline (pre-influenza) data included a serum determination for influenza a and b antibodies, family functioning as measured by the family adaptability and cohesion evaluation scales (faces) ii and the family apgar, and par ...19892715769
protein and nucleic acid analysis of influenza b viruses isolated in italy in 1984.genomic and phenotypic analysis of 30 influenza b viruses, isolated in italy in 1984, antigenically closely related to the b/ussr/100/83 prototype virus, was carried out using t1-oligonucleotide fingerprinting of total rna and one-dimensional peptide mapping. the results of fingerprinting analysis indicated an oligonucleotide spot homology of 90-96%, corresponding to a nucleotide sequence variation of only 0.75-0.3%. all the strains appear to belong to the same evolutionary line. nevertheless, h ...19892723613
sequence of a cdna clone of the nucleoprotein gene of influenza b/ann arbor/1/86. 19892726497
cross-protection against microvariants of influenza virus type b by vaccinia viruses expressing haemagglutinins from egg- or mdck cell-derived subpopulations of influenza virus type b/england/222/82.b/singapore/222/79-like influenza viruses isolated from three patients during the winter of 1981 to 1982 and cultured in either embryonated hens' eggs or mdck cells were studied. sequence analysis indicated that the haemagglutinin (ha) genes of the six virus preparations contained at least four distinct ha1 sequences which differed by up to six amino acids. only one pair of viruses had amino acid differences between the egg- and mdck cell-derived viral subpopulations and this change did not affe ...19892732722
a human melanoma cell line highly susceptible to influenza c virus.the relative amounts of influenza c virus-specific receptors of 25 established lines of mammalian cells including four lines of human malignant melanoma origin were compared by virus binding experiments. all the human melanoma cell cultures studied possessed two to four times more receptors than were found on mdck cells, a cell line known to be highly susceptible to influenza c virus. it may therefore be a feature common to human melanoma cells that o-acetylsialic acid, a determinant for the att ...19892738578
fatal rhabdomyolysis following influenza infection in a girl with familial carnitine palmityl transferase deficiency.severe rhabdomyolysis following an influenza b infection developed in a previously well 13-year-old girl. there was no history of trauma. her course was complicated by episodes of severe hyperkalemia, hypocalcemia, hyperphosphatemia, and myoglobinuria. renal failure, hypertension, and life-threatening arrhythmias developed; she died. muscle biopsy revealed that this girl had carnitine palmityl transferase deficiency. an asymptomatic sister was demonstrated to have the same disorder. although car ...19892748260
national advisory committee on immunization (naci). 19892758483
comparison of the three large polymerase proteins of influenza a, b, and c viruses.the three large rna segments of influenza c virus c/jj/50 were cloned and sequenced, and the deduced amino acid sequences were compared with those of the polymerase (p) proteins of influenza a and b viruses. the coding strategy of the c virus rna segments is the same as that for the large a and b virus segments as one long open reading frame is present in each segment. rna segment 1 of influenza c virus encodes the equivalent of the pb2 protein; it has an approximate 25% sequence identity with t ...19892763462
serological evidence for influenza virus activity in nigeria (1985-1987).a study to determine the activity of influenza virus among the children population was undertaken between 1985 and 1987 in nigeria. paired sera were obtained from children presenting with respiratory diseases at the children emergency and outpatient clinics of the university college hospital, ibadan. forty-two paired sera were examined by haemagglutination-inhibition test to determine hi antibody level of the patients to different serotypes of influenza virus. ninety percent of the children show ...19892773309
molecular hybridization with dna probes as a laboratory diagnosis test for influenza viruses: further investigations on the possibilities of the method.the possibilities of the molecular hybridization test, i.e. test, i.e. using neuraminidase probes of different subtypes for subtype specific detection of influenza, as well as different probes (pa, m, np) for type a specific detection of influenza viruses in infected cells were shown. the results of molecular hybridization in washings' analyses from patients during the outbreaks give us an opportunity to make some conclusions about the usefulness of the method in epidemic control of influenza mo ...19892773310
the use of intravenous ribavirin to treat influenza virus-associated acute myocarditis.we studied three patients with influenza virus-associated fulminant myocarditis; one was infected by type b and the others by type a influenza virus. in one patient, dissemination of type a (h1n1) virus to the myocardium was demonstrated, and viremia complicated the clinical course despite the use of oral amantadine hcl and ribavirin aerosol. all patients were treated with iv ribavirin, two initially and the third after viremia was detected during hyperacute rejection of a cardiac transplant. no ...19892775346
enhancement of protective antibody responses by cholera toxin b subunit inoculated intranasally with influenza vaccine.effects of the b subunit of cholera toxin (ctb) on the primary antibody responses to influenza virus a/pr/8/34 (pr-8) (h1n1) ha vaccine and on protection against viral challenge were investigated in balb/c mice which were immunized intranasally with both the vaccine and ctb. the dose of ctb (greater than or equal to 1 microgram) inoculated with the vaccine (greater than or equal to 0.15 microgram) induced high responses of both antiviral iga antibodies in the nasal wash and haemagglutinin-inhibi ...19892781859
attenuation and phenotypic stability of influenza b/texas/1/84 cold-adapted reassortant virus: studies in hamsters and chimpanzees.the temperature sensitivity, level of attenuation, and phenotypic stability of an influenza b/texas/1/84 x b/ann arbor/1/66 cold-adapted (ca) reassortant virus that received the rna segments that encode the hemagglutinin and neuraminidase surface glycoproteins from the b/texas/84 wild-type virus and the remaining six rna segments from the b/ann arbor/66 ca donor virus were evaluated. in comparison to wild-type virus, the ca reassortant was restricted in replication in the upper and lower respira ...19892794557
sequence analyses of thogoto viral rna segment 3: evidence for a distant relationship between an arbovirus and members of the orthomyxoviridae.the genome of thogoto (tho) virus, an unclassified tick-borne virus, comprises six segments of single-stranded rna. the complete sequence of the third largest rna segment has been determined from overlapping cdna clones and by primer extension studies. segment 3 rna consists of 1865 nucleotides (approx. 6.2 x 10(5) mr). it has a large open reading frame (orf1;597 amino acids, 68.6k) in its virus-complementary sequence, confirming that the rna has a negative-sense coding strategy. a transcription ...19892794982
detection of influenza virus by centrifugal inoculation of mdck cells and staining with monoclonal antibodies.two methods for detection of influenza virus in 451 clinical respiratory specimens were compared: (i) 24-well-plate centrifugation with madin-darby canine kidney (mdck) cells and staining with monoclonal antibody pools to influenza viruses a and b (centers for disease control, atlanta, ga.) in an indirect immunofluorescence assay after incubation for 40 h, and (ii) conventional tissue cell culture with primary monkey cells and hemadsorption. for 100 of these specimens, direct examination of smea ...19892808674
family function, stress, and influenza. 19892809518
relation of chronic disease and immune response to influenza vaccine in the elderly.the ability of elderly patients to mount an adequate immune response to influenza vaccine has been debated. we studied the serum haemagglutination inhibition (hi) antibody response in elderly persons to determine whether different degrees of chronic illness were a critical factor in immune response. in autumn 1986, trivalent split virus vaccine was used to immunize 87 healthy ambulatory elderly adults and 53 institutionalized elderly adults. the pre-vaccination health status of the healthy elder ...19892815966
protection against influenza virus infection by a two-dose regimen of nasal vaccination using vaccines combined with cholera toxin b subunit.the effectiveness of the two-dose regimen, composed of a primary intranasal inoculation of influenza a-type virus ha vaccine together with b subunit of cholera toxin (ctb) and the subsequent intranasal inoculation of vaccine alone 4 weeks later, was examined. in mice given a relatively high dose of virus a/pr/8/34 (pr-8, h1n1) ha vaccine (1.5 micrograms) both as a primary antigen with ctb (1 microgram) and as the second antigen, the secondary responses of both antiviral iga antibodies in nasal w ...19892815967
immune response to trivalent inactivated influenza vaccine in young and elderly subjects.the antibody response (determined using the single radial haemolysis in gel technique) to inactivated whole-virion trivalent influenza vaccine [a/leningrad/360/86(h3n2), a/taiwan/5/87 and b/ann arbor/1/86], recommended for the 1987-88 winter season in italy, in 49 elderly (age greater than or equal to 60 years) subjects was compared with the response in 23 young adult (age less than 60 years) volunteers. the subjects were prevalently healthy and a high percentage of young and old people had been ...19892815969
broad-spectrum in vivo antiviral activity of 7-thia-8-oxoguanosine, a novel immunopotentiating agent.a novel immunopotentiating agent, 5-amino-3-beta-d-ribofuranosylthiazolo [4,5-d]pyrimidine-2,7(3h,6h)-dione (7-thia-8-oxoguanosine), lacks virus-inhibitory properties in vitro but induces interferon and potentiates immune functions, such as natural killer cell activity. it was evaluated in rodent models to determine the spectrum of antiviral activity and effective treatment regimens. at 50 to 200 mg/kg given as single or divided intraperitoneal (i.p.) doses 1 day before virus inoculation, signif ...19892817849
respiratory viruses in children admitted to hospital in edinburgh 1972-1985.the temporal incidence of respiratory viruses identified in children admitted to an edinburgh hospital over a period of 14 years, 1972-1985, is described, respiratory syncytial (rs) virus was identified most often, usually (but not in 1979 and 1985) with single peaks of activity in the winter months. influenza virus infections were seen in the winter and spring but we failed to find evidence of interference between rs and influenza virus. of the viruses studied, the parainfluenza viruses were th ...19872822808
[circulation of influenza viruses in northeast romania during the year 1986]. 19872825319
viral infections in pediatric bone marrow transplant patients.the records of 96 pediatric patients with aplastic anemia or a malignancy who underwent bone marrow transplantation between 1979 and 1986 at the children's hospital of philadelphia were reviewed for laboratory evidence of viral infections. the most common viral diseases identified were herpes simplex virus (hsv), cytomegalovirus and adenoviruses, which were found in 19 (20%), 17 (18) and 17 (18) patients, respectively. hsv was more common in patients with than without graft vs. host disease (gvh ...19882830586
selective inhibition of wsn influenza virus haemolysis by pea lectin.the capacity of lectins to inhibit viral haemolysis of chicken erythrocytes was tested, to evaluate the role of carbohydrate in the fusion reaction. pretreatment of cells with pea lectin provided a 70% to 85% haemolysis inhibition with wsn influenza virus, but only 10% to 14% with pr8 influenza virus. pea lectin did not detectably bind to virus, nor did it inhibit virus binding to cells, but it did inhibit wsn influenza virus elution. additionally, pea lectin was active against sendai virus and ...19882832534
protein synthesis by hepg2 cells infected with influenza b virus.reye's syndrome is an acute hepatopathy and encephalopathy affecting children during the convalescent period of a viral infection, frequently influenza b. the role of influenza b in the pathogenesis of reye's syndrome is unknown. to investigate this relationship, an in vitro system was designed to examine the interaction of influenza b virus with a cell line of human hepatocytes (hepg2) which retains many specific hepatic synthetic characteristics. hepg2 was capable of supporting a productive in ...19882832818
[immunological characteristics of viral myocarditis].a study of cellular and humoral immunity was conducted in patients with viral myocarditis. quantitative and functional deficiency of t-suppressors and an increase in the number of cytotoxic lymphocytes were revealed in most patients. disorder in the t-cellular link of immunity correlated with a clinical course of the disease and was found more frequently in patients with a grave course of myocarditis.19882839908
upper respiratory tract infection and serum antibody responses in nursing home patients.residents of a veterans administration nursing home care unit (nhcu) were observed for the development of upper respiratory tract infection (uri) during 12 consecutive months to determine the frequency of sporadic cases or outbreaks of uri and to characterize them clinically and by laboratory means. fifty-nine episodes of uri occurred in 56 residents during the study period. serologic testing or virus isolation proved or suggested an etiologic agent on 22 occasions. uri was more common in late f ...19882847597
the presence of both antivirus and antiself antibodies in sera from patients with adenovirus and influenza b.using the elisa method we examined serum samples from 62 male patients aged 19-23 infected with adenovirus (serotype 7), 22 children aged 7-14 infected with influenza b (b/norway 1/84) and 113 normal subjects aged 5-30. the infections were diagnosed serologically by complement fixation, by inhibition of hemagglutination, by elisa and by viral culture. moreover using enzyme-linked short-time culture assay, the production of specific antivirus antibodies and autoantibodies in vitro by spleen cells ...19882848359
epidemiology of influenza a and b viruses. use of monoclonal antibodies to analyze viruses from circumscribed epidemics. 19872856221
detection of type a and b influenza viruses in clinical materials by immunoelectronmicroscopy.direct immunoelectronmicroscopy (iem) was used for detecting influenza subtype a(h1n1), a(h3n2) and type b viruses in nasopharyngeal washings or swabs collected during three consecutive periods of enhanced influenza incidence. virus identification was performed with immune rat sera and in the case of the a(h3n2) subtype also with convalescent human sera. in all the materials examined influenza virus was demonstrated by isolation in chick embryos or by immunofluorescence in infected tissue cultur ...19852859758
subunit influenza virus vaccine grippovac se-azh (vaccination of adults).grippovac se-azh a polytype, subunit influenza virus vaccine containing h1n1 and h3n2 influenza a virus strains and one influenza b virus strain has been tested in 80 volunteers. the trials demonstrated the harmlessness, the absence of adverse reactions, and except of the b type, the high antigenicity of vaccine preparation when administered in two injections. the optimal dose of each viral strain haemagglutinin (ha) was 15 micrograms in 0.5 ml.19852864825
monoclonal antibodies for the rapid diagnosis of influenza a and b virus infections by immunofluorescence.mouse monoclonal antibodies, directed against antigenic sites on influenza a and b viruses and found to be type-specific in an immunoassay, were assessed for use as diagnostic reagents in an indirect immunofluorescence assay on nasopharyngeal secretions. the influenza a antibodies were directed against nucleoprotein or matrix protein antigens and the influenza b antibodies against nucleoprotein and haemagglutinin antigens. the influenza a anti-matrix monoclonal antibody was found to give a stron ...19852865418
enzymological heterogeneity of influenza b virus neuraminidase demonstrated by the fluorometric assay method.the neuraminidase activity of 26 strains of influenza b virus isolated from all over the world was investigated colorimetrically, using fetuin as a substrate, and fluorometrically, using 4-methylumbelliferyl(4-mu)-n-ac-alpha-d-neuraminide as a substrate, with special reference to enzymological heterogeneity. the activity of influenza a viral neuraminidases and of a commercially available pure viral one was strongly inactivated by either ethylenediaminetetraacetic acid or glycoletherdiaminetetraa ...19852867656
complete sequence of the major nucleocapsid protein gene of human parainfluenza type 3 virus: comparison with other negative strand viruses.the sequence of the major nucleocapsid protein (np) mrna and its encoded protein were deduced by sequencing a cdna clone representing the complete mrna. the cdna sequence was confirmed by dideoxynucleotide sequencing of purified viral genomic rna by primer extension using synthetic oligonucleotides. the np mrna contains 1,641 nucleotides exclusive of poly(a) and encodes an np protein of 515 amino acids. alignment of the human parainfluenza type 3 virus (pf3) np protein sequence with that of send ...19862878059
influenza vaccination with adjuvant ru41740 in the elderly. 19872882355
influenza virus depresses the pfc response of mice by affecting t-cell function.five influenza virus strains of type a and one strain of type b were used to elicit suppressive effect on the formation of antibody producing cells in response to sheep red blood cells (srbc) both in vivo and in vitro. not only live virus but also the formalin inactivated one exerted immunosuppression (is). although intranasal infection only could cause disease in mice, it exerted no is which, in contrast, occurred after intraperitoneal or intravenous injection of the virus. in nude mice these e ...19872888284
preparation of influenza b virus recombinant strains.the study of antigenic and biologic properties of influenza b epidemic viruses isolated in 1979 and 1983 and laboratory strain b/lee/40 has revealed some differences in their biologic properties. the most marked changes have been found in the haemagglutinin (ha) and neuraminidase (na) indicating that influenza b viruses underwent dramatic antigenic drifts during the period in question. the strains obtained by genetic recombination have inherited surface antigens of epidemic influenza b/singapore ...19872894141
nebulised ribavirin for influenza b viral pneumonia in a ventilated immunocompromised adult. 19882903314
functional and structural analysis of the ribonucleoprotein complexes of different human influenza virus strains.ribonucleoprotein (rnp) cores were prepared from various strains of human influenza virus by treating the purified or spikeless virus particles with non-ionic detergents such as nonidet p-40 and centrifugation in continuous linear glycerol gradients. in addition to rna, the purified complexes contained viral nucleoprotein (np) and the three p proteins (pa, pb1, pb2) as determined by polyacrylamide gel electrophoresis (page) under denaturing conditions. contaminations with other viral polypeptide ...19882903632
antibodies to polymerase proteins of influenza virus a/pr/8/34 (h1n1): comparison on the immunoreactivity with polymerase proteins of other influenza a and b virus strains.the polymerase proteins (pb1, pb2, pa) of the influenza virus strain a/pr/8/34 (h1n1) were isolated from whole virion or ribonucleoprotein (rnp) fractions by electrophoresis on polyacrylamide gel and electroelution or sepharose cl-6b chromatography in the presence of sds. antisera to polymerase proteins (p proteins) were raised in rabbits; the immunoglobulins (ig) were purified by affinity chromatography. characterization of the antibody fraction by western blot analysis showed a highly monospec ...19882903633
interaction between anti-influenza viral polymerase antibodies and rnp particles using the in vitro transcription process and an immunogold labelling technique.immunogold labelling and in vitro transcription of influenza virus vrna have been used to analyse the interaction of anti-influenza polymerase antibodies with influenza-ribonucleoprotein (rnp) complexes. the polymerase proteins (p proteins) were localized exclusively at one end of the rnp segments. in the course of transcription the amount of p protein decreased significantly. the in vitro transcriptase activity y of influenza a virus rnp complexes in the presence of anti-polymerase antibodies t ...19882904734
effect of mutations and deletions in a bicistronic mrna on the synthesis of influenza b virus nb and na glycoproteins.the mrna derived from influenza b virus rna segment 6 is functionally bicistronic and encodes the nb and na glycoproteins in different, overlapping reading frames. nb protein synthesis is initiated at the 5'-proximal aug codon, and 4 nucleotides downstream there is a second aug codon which is used to initiate na protein synthesis. the nucleotide sequence context of the first aug codon conforms closely with the established 5'-cc(a/g)ccaugg-3' consensus sequence (m. kozak, nucleic acids res. 15:81 ...19892908923
leads from the mmwr. update: influenza activity--worldwide. 19892911154
[specific and nonspecific indicators of activation of influenza viruses before an epidemic caused by influenza viruses subtype a/h3n2 and type b in czechoslovakia in 1986].the authors submit an aetiological and epidemiological analysis of the influenza epidemic which occurred in the csr between the 4th and 14th week of 1986 and was caused by the influenza virus subtype a/h3n2/ and type b. the epidemic affected a total of 27.1% of the population, in the age group of 0-5 years 63.7%, in the age group 6-14 years 52.7% and in the age group above 15 years 17.1%. in the course of the epidemic 77,458 cases of pneumonia and bronchitis were reported and 1,412 deaths with t ...19892920398
leads from the mmwr. influenza vaccine composition recommendation for the 1989-90 season. 19892926955
[immunogenicity and the protective effectiveness of the subunit trivalent influenza vaccine grippovac].in the process of the immunological approbation of several experimental batches of the triyalent subunit influenza vaccine grippovac, the pronounced immunogenicity of the antigens of all strains contained in the vaccine was established; most of the vaccinees were found to retain sufficiently high antibody titers for a year, and the essential total increase of antibody titers was found to occur after a single booster immunization. the serological checking of the diagnosed cases of influenza among ...19862938372
[influenza morbidity in the kazakh ssr 1977-1984].in 1977-1984, 6 epidemic outbreaks of influenza were recorded in the republic of which 2 were due to influenza a (h1n1), 2 to a (h3n2) and 2 to influenza b virus. the epidemics of influenza h1n1 (1977-1978, 1981) and h3n3 (1979-1980, 1983) were characterized by gradual decrease of their intensity as manifested by lowering of the morbidity rate and frequency of virus isolation. on the contrary, epidemics of influenza b showed a certain trend to increase. an important feature of the epidemics etio ...19862945325
[epidemiological surveillance of influenza based on research data on collective immunity].in 1976-1984 the dynamic study of communal immunity to influenza a and b virus strains was made with the aim of the etiological prognostication of influenza epidemic. on the basis of the study of the immune status of the adult and child population to the virus strains, actually circulating in october-november, the possibility of prognosing the tendency in the development of the epidemiological situation in influenza in the following year was established.19862948353
[relation between features of the clinical course of influenza and the dynamics of the epidemic process]. 19852951900
[epidemiologic and clinical characteristics of influenza among the children of volgograd]. 19852951904
[the results of state trials of inactivated influenza vaccines for children].controlled epidemiological surveillance covering the total number of 13,355 schoolchildren aged 11-14 years and adolescents was carried out with a view to compare the efficacies of inactivated influenza vaccines. the children were immunized intradermally in a single injection with inactivated influenza vaccines containing a (h3n2) and a (h1n1) hemagglutinins, 3.5 micrograms per 0.2 ml of the preparation each. the studies demonstrated the safety and low reactogenicity of these vaccines, as well a ...19882968738
two nuclear location signals in the influenza virus ns1 nonstructural protein.the ns1 protein of influenza a virus has been shown to enter and accumulate in the nuclei of virus-infected cells independently of any other influenza viral protein. therefore, the ns1 protein contains within its polypeptide sequence the information that codes for its nuclear localization. to define the nuclear signal of the ns1 protein, a series of recombinant simian virus 40 vectors that express deletion mutants or fusion proteins was constructed. analysis of the proteins expressed resulted in ...19882969057
the influence of human immunodeficiency virus (hiv) infection on antibody responses to influenza vaccines.to ascertain whether subjects infected with human immunodeficiency virus (hiv) generally develop protective hemagglutination inhibition antibody responses to inactivated influenza vaccines.19882970238
biological functions of the ns1 protein of an influenza b virus mutant which has a long carboxyl terminal deletion.to clarify the function of the ns gene of a highly cytolytic mutant of influenza virus b/yamagata/1/73 which expresses an ns1 protein with a long carboxyl terminal deletion (clone 201), we prepared a single gene reassortant (201 l-77) and a control reassortant (yl-20) in which all the genes were of wild type influenza virus b/lee/40 origin except ns gene which was derived from either clone 201 or wild type b/yamagata. comparative studies have revealed that 201 l-77 destructed infected cells more ...19882974275
sequence determination of the sendai virus fusion protein gene.from a genomic dna library of sendai virus, we have identified and sequenced clones corresponding to the f glycoprotein gene. the limits of the f gene region were defined by mapping the 5' and 3' ends of the mrna with s1 nuclease. the sendai virus f gene is 1821 nucleotides long. the predicted primary translation product of the single long open reading frame would code for a protein of 565 amino acids, containing a putative signal peptide, three carbohydrate addition sites, a hydrophobic region ...19852981971
biochemical techniques for the genetic and phenotypic analysis of viruses: 'molecular epidemiology'. 19852982941
[immunoprecipitation, with an antiserum to ovalbumin, of protein np from influenza a virus and of glycoprotein c from the herpes simplex type i virus].antisera to ovalbumin and to thyroliberin react in radioimmunoprecipitation and radioimmunoassay with np protein of influenza a virus and c glycoprotein of herpes simplex virus whose mutual amino acid homology does not practically exceed the limits of homology in tripeptides. both proteins contain a tripeptide area corresponding to thyroliberin tripeptide.19852986359
expression of influenza virus ns2 nonstructural protein in bacteria and localization of ns2 in infected eucaryotic cells.the nonstructural ns2 protein of influenza a/pr/8/34 virus was efficiently expressed in bacteria, and monospecific antisera were prepared against the bacterially synthesized polypeptide. these antisera were cross-reactive among the ns2 proteins of various influenza a viruses. however, they did not react with the ns2 of influenza b/lee/40 virus nor with other proteins of influenza a viruses such as ns1. antisera against ns2 were used to determine that the ns2 protein is localized in the cell nucl ...19852987535
epidemiological features of influenza in a large town of romania during 1983.data supplied by the active influenza surveillance - including clinical, epidemiological and laboratory investigations - allowed the characterization of the particularities of an influenza outbreak caused by a(h3n2) virus in a large town of romania in march 1983. the epidemiological impact of influenza in 1983 is discussed in the light of the complex relationships between the antigenic structure of circulating influenza strains and the profile of antiinfluenza immunity of the population.19852988190
surveillance of respiratory viral infections by rapid immunofluorescence diagnosis, with emphasis on the epidemiological development of respiratory syncytial virus infections.surveillance of certain respiratory viral infections by applying immunofluorescence (if) examinations to samples of nasopharyngeal secretions has been evaluated using a simplified procedure for the preparation of cell smears. samples from 711 children living in different parts of norway were examined during the winter 1982/83 and a positive diagnosis was made for 290 children (41%). temporal epidemic peaks were observed for respiratory syncytial virus (rsv), parainfluenza virus type 3 and influe ...19852989359
arginine deficiency, hyperammonemia and reye's syndrome in ferrets.young male ferrets developed hyperammonemia and encephalopathy shortly after eating a diet lacking in arginine. the dietary supplementation of arginine or intraperitoneal injection of ornithine prevented hyperammonemia and shortened the duration of encephalopathy. therefore, young ferrets were assumed to be unable to meet their ornithine needs from sources other than arginine. adult ferrets did not develop hyperammonemia and encephalopathy after eating arginine-free diet. because young ferrets a ...19852991654
rapid viral diagnosis of acute respiratory infections: comparison of enzyme-linked immunosorbent assay and the immunofluorescence technique for detection of viral antigens in nasopharyngeal secretions.nasopharyngeal secretions from adults and children were obtained in stockholm, sweden, for routine diagnosis of influenza a virus, influenza b virus, respiratory syncytial (rs) virus, parainfluenza type 3 virus, and adenovirus infections by demonstration of viral antigens directly in the specimens. the cells in nasopharyngeal secretions were pelleted by centrifugation for preparation of cell deposits for diagnosis by the immunofluorescence technique (if) in london, england, and in stockholm, whe ...19852997270
persistence of viruses in the nasopharynx of apparently healthy children aged 0-5 years. results of investigations performed in 1982-83.virus isolation attempts were made with nasopharyngeal secretions from 400 apparently healthy 0-5-year-old children of a semi-closed community. repeated virus isolations were achieved in 25 cases subjected to periodic investigations in 1982-83. one-two reisolation(s), 1-5 months apart, of the initially detected viral agents could be obtained in 15 out of the 25 children. the following viruses were reisolated (in decreasing order of frequency): adenoviruses, respiratory syncytial virus, coxsackie ...19853000066
[pathogenetic and pathomorphological problems of viral-bacterial associations in children dying of meningococcal infection].the information on 63 children dying from hypertoxic forms of meningococcal infection is presented. four groups of brain damage by respiratory viruses (rv) are distinguished on the basis of the results of morphological and virological examination: 1) with a recent rv generalization (26 cases); 2) with dissemination of an etiological agent but without clear-cut structural changes (6 cases); 3) with an isolated affection of the brain (13 cases); 4) without clear-cut brain damage. experimental infl ...19853000321
antiviral properties of garlic: in vitro effects on influenza b, herpes simplex and coxsackie viruses. 19853001801
[viral myocarditis (the etiologic, clinical, diagnostic and treatment problems)].to elucidate the etiology of respiratory infection and pharyngitis associated myocarditis a serological study was made of 201 patients who were successively admitted with a clinical diagnosis of myocarditis. coxsackie viral infection of group b, influenza a and b, para-influenza and adenoviral infection and beta-hemolytical streptococcus of group a were determined. preceding coxsackie infection was established in 38,3% of the patients, influenza a and b in 27.5%, adenoviral infection in 3.6% and ...19853001963
effect of the oral administration of nivgrip inactivated influenza vaccine on virus carriage in the nasopharynx of children aged 0-5 years.the nivgrip inactivated influenza vaccine was administered by oral route to 11 children of a semi-closed pre-school community, found to carry viruses in their nasopharynx. periodic virological investigations demonstrated that after vaccination virus carriage disappeared completely in 7 children; in the remaining 4 cases there was a considerable decrease (from 14 to 6) in the number of virus strains isolated. the virus isolates obtained after vaccination were represented exclusively by enteroviru ...19853004017
virological investigations in adults with acute pneumonia.virological investigations (immunofluorescence reactions and isolation attempts with pharyngeal exudate specimens, as well as serological tests) were performed in 110 adult patients with pneumonia. viral or inframicrobial agents were involved in 70 (63.7%) of the cases, either alone (27 cases) or in association with bacteria (43 cases). parainfluenza and adenoviruses were most frequently encountered both in the cases with mixed (viral + bacterial) and in those with strictly viral pneumonia. myco ...19863008411
rapid diagnosis of respiratory virus infections in patients with acute respiratory disease.viral respiratory infections represent a significant segment of the total respiratory disease spectrum; however, until recently the laboratory diagnosis of viral respiratory infections was relatively inefficient. development of new and improved immunologic assay systems has paved the way for accurate and reliable rapid diagnostic tests that detect viral antigens in clinical specimens. we conducted a careful and elaborate study in which radioimmunoassay for antigen detection was compared with a b ...19863009083
viral respiratory disease in the intensive care unit. 19863009357
prevention and control of influenza. 19863010071
presence of respiratory viruses in middle ear fluids and nasal wash specimens from children with acute otitis media.during a 28-month period, 84 children with acute otitis media were studied by viral and bacterial cultures of middle ear fluid and viral cultures of nasal lavage fluid. viruses were isolated from the middle ear fluid of 17 (20%) patients. evidence of viral infection was demonstrated by positive viral cultures of middle ear fluid and/or nasal lavage fluid in 33 (39%) patients. rhinovirus in one patient and influenza b virus in another were the only pathogens isolated. influenza virus, enterovirus ...19863010225
[viral respiratory infections in children: new diagnostic methods for early detection. initial results of a pilot project in switzerland].the new methods of rapid viral diagnosis make it possible to specify a number of the most prevalent agents of respiratory tract infections within 24 hours. the techniques are based on the immunological detection of antigens of respiratory syncytial (rsv), adeno, parainfluenza type 1, 2 and 3, as well as of influenza a and b viruses in nasopharyngeal secretions. during a one-year period we have used these methods to evaluate diagnostically 1541 outpatients presenting with upper and lower respirat ...19863012772
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