Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| esterolytic activities of rat intestinal mucosa. 2. purification and properties of a glycerol-ester hydrolase. | a glycerol-ester hydrolase from rat intestinal cells has been purified using chromatography on carboxyhexanoyl-sepharose-glyceryldioctanoate and preparative gel electrophoresis. the enzyme gives a single band by analytical gel electrophoresis; it is a monomer of molecular weight 68000. the optimum ph for its action on glyceryl tributyrate is between 8.0 and 8.5; the activation energy was calculated to be 8.7 kcal x mol-1 (36.4 kj/mol). its substrate specificity is mainly directed against esters ... | 1976 | 12957 |
| stopped-flow spectrophotometric characterization of enzymic reaction intermediates in the anaerobic reduction of pig-plasma benzylamine oxidase by amine substrates. | reduction of benzylamine oxidase by p-methoxybenzylamine under anaerobic conditions leads to biphasic absorbance changes at 470 nm. these reflect the intermediate formation of an enzyme substrate complex with spectral properties different from those of native enzyme and fully reduced enzyme. the spectrally modified enzyme-substrate complex exhibits a broad difference absorption band centered around 360 nm. the transient accumulation of this intermediate during reaction can be conveniently follow ... | 1976 | 12963 |
| kinetics of the interaction between aspartic aminotransferase and anions. | the kinetics of the interaction between deionized supernatant aspartic aminotransferase and various anions (cacodylate, phosphate and chloride) were studied by the temperature-jump technique. the anion concentration in the range covered by our experiments does not affect the transamination rate. on the other hand the conformational transition, recently observed at the active site of the enzyme, is hindered by an excess of anions. a single relaxation effect was observed at the enzyme chromophore ... | 1976 | 12967 |
| the dissociation of flavin coenzymes from trypsin-solubilized nadph/cytochrome c (p-450) reductase of pig-liver microsomes. | the change in fluorescence emission at 520 nm after excitation at 365 nm was used to investigate the effect of ph and ionic strength on the dissociation of flavin cofactors from microsomal nadph/cytochrome c (p-450) reductase. in the unmodified enzyme both the fad and fmn moieties appeared to dissociate at a similar rate and followed first-order kinetics. the rate constant for the dissociation was increased by low ph and high ionic strength, particularly in the range ph 4.4-3.8 (0.02 m acetate b ... | 1976 | 12969 |
| c-terminal fragment (residues 61-91) of beta-lipotropin: is it the natural opiate-like neurohormon of the brain? | 1977 | 12995 | |
| studies on the cyclic 3':5'-amp-stimulated pig liver protein kinase reaction with pyruvate kinase as substrate. | the phosphorylation of pig liver pyruvate kinase by cyclic adenosine 3':5'-monophosphate-dependent protein kinase has been studied. for comparison, mixed histone and a synthetic heptapeptide were also used as substrates. protein kinase was purified by chromatography on deae-cellulose, hydroxyapatite, and sephadex g-200. the enzyme was stimulated by cyclic amp with apparent ka values of 2.5 and 0.8 x 10-7 m for pyruvate kinase and histone substrates, respectively. divalent cations were essential ... | 1977 | 13074 |
| investigation of the subunit interactions in malate dehydrogenase. | the dissociations of porcine heart mitochondrial, bovine heart mitochondrial, and porcine heart cytoplasmic malate dehydrogenase dimers (l-malate: nad+oxidoreductase, ec 1.1.1.37) have been examined by sephadex g-100 gel filtration chromatography and sedimentation velocity ultracentrifugation. the porcine mitochondrial enzyme was found to chromatograph as subunits when applied to a gel filtration column at a concentration of .02 mum or less at ph 7.0. the presence of coenzymes shifted the dissoc ... | 1977 | 13078 |
| guanylate cyclase and cyclic guanosine monophosphate in the guinea-pig cochlea. | 1977 | 13157 | |
| contractile activity of synthetic neurotensin and related polypeptides on guinea-pig ileum. | 1977 | 13189 | |
| the interaction of histamine and guanylnucleotides with cardiac adenylate cyclase and its relationship to cardiac contractility. | histamine stimulates adenylate cyclase activity in a washed membrane preparation from guinea-pig ventricle. marked synergistic effects are observed with histamine and gtp. in the absence of gtp, the degree of stimulation of the enzyme by histamine is slight and occurs only in the presence of relatively high concentrations of atp suggesting that atp, or contaminating gtp in commercial preparations of atp, may partially satisfy the guanylnucleotide requirement. the gtp analog, gppnhp, strongly and ... | 1977 | 13198 |
| propoxyphene and norpropoxyphene: pharmacologic and toxic effects in animals. | alpha-d-propoxyphene and its principle metabolite, alpha-d-norpropoxyphene, were compared pharmacologically to establish their relative opioid profiles as defined by naloxone reversal. propoxyphene exhibited opioid activity in the following tests: mouse abdominal constriction and rat tail heat analgesic tests, inhibition of the twitch of the guinea-pig ileum and acute lethality in rodents. norpropoxyphene also showed opioid activity in the rat tail heat and guinea-pig ileum tests, but exhibited ... | 1977 | 13200 |
| direct measurement of the intracellular ph of mammalian cardiac muscle. | 1. the intracellular ph (phi) of sheep heart purkinje fibres and rat, ferret and guinea-pig ventricle has been measured using recessed-tip ph-sensitive micro-electrodes. 2. in the absence of co2 the phi was approximately 7-2 in all the preparations used. in 5% co2 the mean phi was 7-14 in rat and ferret ventricle and 7-02 in sheep purkinje fibres. 3. the phi response to an increase or a decrease in the co2 level (at constant external ph) was biphasic with a large transient change followed by a p ... | 1976 | 13205 |
| molecular and genetic basis of furocoumarin reactions. | 1976 | 13299 | |
| beta-adrenergic receptors in guinea-pig myocardial tissue. | stereospecific binding sites for (-) 3h-alprenolol, a beta-adrenergic antagonist, have been identified in guinea-pig myocardial broken cell preparations. the concentration of the sites was 0.3 pmoles per mg of protein and the dissociation constant (at 37 degrees c) 10(-8) m. a close correlation between the ability of various beta-adrenergic antagonists to compete with tracer alprenolol binding and to block the response of isoprenaline-stimulated myocardial adenylate cyclase has been found. low a ... | 1976 | 13314 |
| electromechanical studies of an anemonia sulcata toxin in mammalian cardiac muscle. | 1. in guinea-pig papillary muscles toxin ii isolated from the sea anemone anemonia sulcata (atx) enhanced the force of contraction without changing the time-to-peak tension. atx increased the relaxation time. the positive inotropic effect was accompanied by a prolongation in action potential duration. the action potential amplitude and the resting membrane potential remained essentially unchanged. the velocity of depolarization (dv/dtmax) decreased slightly. the effects werenot modified by pretr ... | 1976 | 13316 |
| immunofluorescence histochemistry of porcine tissues using antibodies to pig plasma amine oxidase. | 1977 | 13405 | |
| the role of cyclic amp in the activation of tyrosine hydroxylase during nerve stimulation proceedings. | 1977 | 13451 | |
| mechanism of the leukocyte agglomeration reaction (lar) with tissue heteroantigens in pulmonary tuberculosis. | 1976 | 13575 | |
| antihistaminic and local anesthetic effects of benzhydryl-beta-(hexahydrooxazocino-1,5)ethyl ether. | 1976 | 13608 | |
| culverts and trypanosome transmission in the serengeti national park (tanzania). i. survey of the culverts. | 1976 | 13634 | |
| culverts and trypanosome transmission in the serengeti national park (tanzania). iii. studies of the genus auchmeromyia bauer and bergenstamm. (diptera: calliphoridae). | 1976 | 13636 | |
| pig liver fatty acid synthetase: purification and physicochemical properties. | 1977 | 13730 | |
| inhibition of prostaglandin biosynthesis by non-narcotic analgesic drugs. | the existence of a relationship between inhibition of prostaglandin biosynthesis and analgesic or anti-inflammatory activity was investigated in the case of the non-narcotic analgesics glafenine, floctafenine and clometacine, in comparison to indomethacin and acetylsalicylic acid. these compounds inhibit prostaglandin biosynthesis from arachidonic acid in a guinea-pig lung homogenate as strongly as indomethacin. on its biosynthesis in rat epididymal tissue stimulated by noradrenaline, glafenine ... | 1976 | 13749 |
| selectivity of clenbuterol (nab 365) in guinea-pig isolated tissues containing beta-adrenoceptors. | the effects of clenbuterol (anb 365), an aminohalogen substituted phenylethanolamine, have been examined on isolated tissue preparations from guinea-pigs. clenbuterol produced concentration (or dose)-dependent relaxations of tracheal chains, decreases in perfusion pressure of hind limb blood vessels, inhibitions of acetylcholine-induced contractions of the uterus, increases in atrial rate and inhibitions of electrically-induced contractions of the ileum. these responses were blocked by propranol ... | 1976 | 13750 |
| transamination reactions in the thyroid gland. | 1976 | 13788 | |
| bis-(4-methylumbelliferyl) phosphate as a substrate for the surface membrane-associated phosphodiesterase activity of pig platelets. | it was found that the newly-available compound, bis-(4-methylumbelliferyl) phosphate, could be used as a substrate for the pig platelet surface membrane-associated phosphodiesterase activity, usually assayed with bis-(p-nitrophenyl) phosphate. this enzyme activity is distinct from the phosphodiesterase activity towards 5'-dtmp-p-nitrophenyl ester, which is probably associated with intracellular membrane structures in platelets. consequently, the use of the 4-methylumbelliferyl derivative as subs ... | 1977 | 13836 |
| effects of divalent cations and sodium taurocholate on pancreatic lipase activity with gum arabic-emulsified tributyrylglycerol substrates. | the effects of ca2+ and/or sodium taurocholate on lipase activity with gum arabic-emulsified tributyrylglycerol substrates were investigated. calcium was found to slightly increase lipase activity while bile salts showed marked inhibition except at very low concentrations. calcium eliminated inhibition seen with low concentrations of bile salts and reduced the inhibition seen at higher bile shift of the enzyme from the alkaline region in the absence of bile salt to the slightly acidic region in ... | 1977 | 13848 |
| solubilised intrinsic factor receptor from pig ileum and its characteristics. | the vitamin b-12-intrinsic factor receptor was shown to be present in pig ileum and localized on the brush borders of the enterocytes, and to be solubilisable with triton x-100. at neutral ph and in the presence of ca2+ it bound the vitamin b-12-intrinsic factor but not the vitamin b-12-cobalophilin complex. the solubilised vitamin b-12-intrinsic factor-receptor complex consisted of two molecular species with clear stokes radii (13.11 and 33.53 nm), sedimentation coefficients (15.1 and 45.1 s) a ... | 1977 | 13861 |
| interactions between the effects of alpha- and beta-adrenoceptor agonists and adenine nucleotides on the membrane potential of cells in guinea-pig liver slices. | 1 the beta-adrenoceptor agonist isoprenaline normally causes only a small and inconsistent increase in the membrane potential of cells in guinea-pig liver slices, in contrast to the large hyperpolarizations seen with alpha-agonists. however, after a selective alpha-adrenoceptor agonist has been applied, the response to isoprenaline becomes greatly enhanced. 2 simultaneous application of small doses of an alpha- and beta-agonist produce hyperpolarizations larger than the sum of the responses to e ... | 1977 | 13900 |
| amino acid composition of casein isolated from the milks of different species. | 1977 | 13923 | |
| preparation of coenzymic activity of soluble polyethyleneimine-bound nadp+ derivatives. | alkylation at n-1 of the nadp+ adenine ring with 3,4-epoxybutanoic acid gave 1-(2-hydroxy-3-carboxypropyl)-nadp+. enzymic reduction of the latter, followed by alkaline dimroth rearrangement and enzymic reoxidation, gave n6-(2-hydroxy-3-carboxypropyl)-nadp+. on the other hand, bromination at c-8 of the nadp+ adenine ring, followed by reaction with the disodium salt of 3-mercaptroproionic acid, gave 8-(2-carboxyethylthio)-nadp+. carbodimide coupling of the three carboxylic nadp+ derivatives to pol ... | 1977 | 13999 |
| aminotetralins as selective beta-adrenergic agonists. | aminotetralin derivatives m-8 and jod-176, were studied in relation to isoproterenol for beta-adrenergic activity. m-8 and jod-176 were found to be very weak in activating cardiac beta-receptors. these compounds were also found to be considerably more active in uterine and bronchial smooth muscle. because these compounds were also shown to be very inactive in vascular smooth muscle, the possibility that beta-receptors in different smooth muscle types may also differ was suggested. | 1977 | 14012 |
| "direct" effect of reserpine on various preparations. | the action of reserpine added to the bathing solution has been studied in isolated organs, examining various kinds of receptor systems and biological preparations. the investigation has been comparatively carried out in normal and reserpine-pretreated animals. the most important effect of reserpine is the inhibition of the maximum response to noradrenalin in the rat vas deferens, and the inhibition of response to furtrethonium and histamine in the intestine of rat and guinea-pig, respectively. t ... | 1976 | 14020 |
| differences in the reactivities of human urokinase and the porcine tissue plasminogen activator. | it has previously been shown, that large differences exist between the effects of 6-aminohexanoic acid or alpha1-antitrypsin on fibrinolysis caused by a porcine tissue plasminogen activator or by human urokinase, while insignificant differences exist between the effects of a number of natural protease inhibitors on fibrinolysis caused by the two types of plasminogen activator. the present study shows that changes in substrate composition (ph, ionic strength fibrinogen concentration, plasminogen ... | 1976 | 14058 |
| species distribution and properties of hepatic phenylalanine (histidine):pyruvate aminotransferase. | hepatic phenylalanine(histidine):pyruvate aminotransferase activity is much higher in the mouse and rat than in other animal species (human, guinea-pig, rabbit, pig, dog and chicken). the activity is elevated in the mouse and rat by the injection of glucagon but not in other species (guinea-pig, rabbit and chicken). the enzyme was purified from the mitochondrial fraction of mouse liver to homogeneity as judged by polyacrylamide disc gel electrophoresis in the presence of dodecylsulphate. with hi ... | 1977 | 14070 |
| mechanisms underlying the absorption of water and ions. | 1977 | 14083 | |
| phosphorylation of cardiac troponin by cyclic adenosine 3':5'-monophosphate-dependent protein kinase. | the purpose of this investigation was to characterize the phosphorylation of bovine cardiac troponin by cyclic amp-dependent protein kinase. the purified troponin-tropomyosin complex from beef heart contained 0.78 +/- 0.15 mol of phosphate per mol of protein. analysis of the isolated protein components indicated that the endogenous phosphate was predominately in the inhibitory subunit (tn-i) and the tropomyosin-binding subunit (tn-t) of troponin. when cardiac troponin or the troponin-tropomyosin ... | 1977 | 14136 |
| interaction of thyroid peroxidase with concanavalin a covalently coupled to agarose. | we have investigated the interaction between concanavalin a-agarose (con a-agarose) and thyroid peroxidase, an integral membrane protein found in the 105,000 x g, 1-h particulate fraction of thyroid tissue. an intact form of porcine thyroid peroxidase was obtained by solubilization with the nonionic detergent triton x-100 and two fragmented, hydrophilic forms of the enzyme were prepared by trypsin treatment of the membrane. the three types of thyroid peroxidase bind to con a-agarose and can be e ... | 1977 | 14148 |
| activation of the alternative pathway by pneumococcal cell walls. | the present studies were performed in order to identify the pneumococcal subcellular component responsible for activating the alternative pathway. purified pneumococcal cell walls were able to activate the alternative pathway at a concentration as low as 5 mug/ml and were more active than crude cell walls, which in turn were more active than the whole organism. purified pneumococcal cell membranes also were able to activiate the alternative pathway but had less than 10% of the activity of the pu ... | 1977 | 14211 |
| ascorbic acid blockade of muscle contractions by neurotransmitters and 2-aminoethanol. | 1977 | 14285 | |
| competitive inhibitors of renin. a review. | this review describes some of the characteristics for renin's substrate specificity and also some features of its species specificity. it describes how competitive inhibitors were synthesized and how the solubility was increased in order to make them effective at physiological ph. their use for in vitro and in vivo inhibition of renin is discussed and their use for purification of renin demonstrated. | 1976 | 14338 |
| inhibition of adrenergic beta receptors by n,n'-bis-3-(2'ethoxyphenoxy)-2-hydroxypropyl-ethylenediamine (falirytmin) as compared to propranolol. | 1976 | 14343 | |
| preparation of 125i-labeled synthetic porcine secretin for radioimmunoassay. | synthetic porcine secretion was labelled by the conjugation-labelling method of bolton & hunter, the lactoperoxidase method, the gaseous diffusion method, and the chloramine-t method. the chloramine-t technique was adapted as routine method. ten mug (3.27 nmol) peptide was reacted with 5 mci of na125i at a concentration of chloramine-t of 1.3 mmol/l. synthetic secretin was suitable for labelling for at least eight months when stored as dry matter in nitrogen-filled glass ampoules. purification a ... | 1976 | 14392 |
| the cardiac actions of a toxin-containing material from the jellyfish, cyanea capillata. | 1977 | 14423 | |
| ionizing radiation and the immune response. | 1976 | 14486 | |
| stimulants: interaction with clinically relevant drugs. | 1976 | 14585 | |
| antidepressants and the muscarinic acetylcholine receptor. | several tricyclic antidepressants have been assessed for their potency in binding to the muscarinic acetylcholine receptor of brain and intestine. amitriptyline hydrochloride is about ten times as potent as imipramine hydrochloride. dimethylated drugs are more potent than monomethylated ones. the relative anticholinergic activities of tricyclic antidepressants have implications for their use in patients who might be affected adversely by anticholinergic effects. | 1977 | 14603 |
| simultaneous determination of skeletal muscle fiber, types i, iia, and iib by histochemistry. | we describe a simplified technique for the histochemical determination of three fiber types from a single section of skeletal muscle. preincubation in a solution of formaldehyde, glycine, and calcium followed by routine myofibrillar adenosine triphosphatase (atpase) incubation clearly differentiates type i, type iia, and iib fibers in human, rat, rabbit, and porcine muscle. in addition, glycine-formaldehyde-calcium preincubation offers better preservation of cytoarchitecture and standardization ... | 1977 | 14607 |
| conformational transition accompanying the binding of ca2+ to the protein activator of 3',5'-cyclic adenosine monophosphate phosphodiesterase. | the ca2+-dependent protein activator of 3':5'-cyclic adenosine monophosphate phosphodiesterase is shown to undergo a conformational transition upon binding of 2 mol of ca2+/mol of activator. circular dichroic studies indicate that ca2+ induces an increase of 5-8% in alpha-helix content with a concomitant decrease in the amount of random coil. in the absence of ca2+ and in the presence of ethylenebis(oxoethylenenitrilo)tetraacetic acid (egta), the protein contains 30-35% alpha helix, 50% random c ... | 1977 | 14663 |
| isotope effect studies of the role of metal ions in isocitrate dehydrogenase. | pig heart nadp+-dependent isocitrate dehydrogenase requires a metal ion for activity. under optimum conditions (ph 7.5, mg2+ present), the carbon isotope effect is k12/k13 = 0.9989 +/- 0.0004 for the carboxyl carbon undergoing decarboxylation and hydrogen isotope effects are vmaxh/vmaxd = 1.09 +/- 0.04 and (vmax/km)h/(vmax/km)d = 0.76 +/- 0.12 with threo-d,l-2-2hisocitric acid. deuterium isotope effects measured by the equilibrium perturbation technique under the same conditions are vh/vd = 1.20 ... | 1977 | 14672 |
| influence of ph and sodium on the inhibition of guinea-pig heart (na+ + k+)-atpase by calcium. | the inhibition of guinea-pig heart (na+ + k+)-atpase (atp phosphohydrolase ec 3.6.1.3) by calcium has been studied at ph 7.4, 6.8 and 6.4. 1. a decrease in ph reduced the threshold inhibitory concentration of calcium and the calcium concentration producing an inhibition of 50% of the enzyme activity. 2. calcium reduced the apparent affinity of the enzyme of na+, this effect occurred only at ph 7.4. 3. calcium increased the apparent affinity of the enzyme for k+, this effect was enhanced at acidi ... | 1977 | 14690 |
| flow rates of components in digesta of pigs prepared with re-entrant cannulas in the proximal duodenum and terminal ileum, and fed semipurified, hard wheat, and soft wheat diets. | four pigs prepared with re-entrant cannulas in the proximal duodenum and terminal ileum were used to study flow rates of total digesta, insoluble dry matter, nitrogen, and amino acids entering and leaving the small intestine. the pigs received a semipurified diet, a hard wheat diet, or a soft wheat diet. these were approximately isonitrogenous. a higher rate of passage of digesta through the proximal duodenum and terminal ileum were measured in pigs receiving the hard wheat diet. peak flow of di ... | 1976 | 14777 |
| studies on the structure activity relationship of adrenergic beta-mimetic benzylamine derivatives. ii. 1-alkylamino-2-phenyl-1,2,3,4-tetrahydronaphthalenes. | 1976 | 14787 | |
| intramolecularly-quenched fluorescent peptides as fluorogenic substrates ofleucine aminopeptidase and inhibitors of clostridial aminopeptidase. | fluorogenic oligopeptide derivatives of the type lys(abz)-onbzl, where abz iso-aminobenzoyl (anthraniloyl), x stands for ala phe, or ala-ala, and onbzlis p-nitrobenzyloxy, were synthesized and shown to be hydrolyzed by leucine aminopeptidase. the hydrolysis is accompanied by an increase in fluorescence due to disruptionof the intramolecular quenching of the fluorescent anthraniloyl moiety by the nitrobenzyester group. the spectral characteristics of the compounds are not consistent withan energy ... | 1977 | 14833 |
| h202 generation in subcellular fractions of leukocytes assayed by cytochrome c peroxidase method. | 1977 | 14848 | |
| enzymatic processes and chemical composition of the cecal contents of swine fed carbohydrates from different plants. | a considerable amount of low-molecular acids (14.5-18.2 meqv/100 ml) is formed in the coecum in consequence of fermentation of cellulose, starch, and other components of chyme with microflora. the total concentration and molecular per cent ratio of the acetic, propionic, butyric, and lactic acids depended on the composition of the carbohydrate ration. | 1976 | 14851 |
| stimulatory (h1) and inhibitory (h2) histamine receptors in gallbladder muscle. | the nature of histamine receptors in gallbladder muscle and examined using specific histamine-receptor agonists and antagonists. the h2-receptor antagonist, metiamide, augmented the contractile response to histamine indicating that gallbladder muscle possessed stimulatory h1 receptors and inhibitory h2 receptors. the independent inhibitory character of h2 receptors was confirmed by (1) induction of relaxation with histamine after h1-receptor blockade and the suppression of this relaxation with m ... | 1977 | 14867 |
| mathematical analysis of ligand-induced monomerization and dimerization in the monomer dimer equilibrium of proteins. application to d-amino acid oxidase. | we have mathematically analyzed ligand-induced monomerization and dimerization in a protein monomer-dimer equilibrium system, in which the monomer has one and the dimer two binding sites. these dimer sites have the same binding constants for the first ligand but may cooperatively interact when one of them is occupied by a ligand molecule. in this system, the apparent dimerization constant and the apparent molecular weight are functions of free ligand concentration, and depend on the intrinsic bi ... | 1977 | 14929 |
| kallikrein inhibitors in rat plasma. | the kallikrein inhibitor contents of human and animal plasma were determined with glandular kallikreins ec 3.4.21.8. one ml of plasma could inactivate 20-700 kallikrein units (ku). rat plasma was the most potent and inactivated 230-700 ku. however, no enzyme capable of inactivating kallikrein could be found in this plasma. two fractions which inhibited hog pancreatic kallikrein, a fraction corresponding to alpha2-macroglobulin and a fraction which was eluted prior to albumin, were separated from ... | 1977 | 14935 |
| tryptic cleavage of rat liver sulfite oxidase. isolation and characterization of molybdenum and heme domains. | treatment of rat liver sulfite oxidase with trypsin leads to loss of ability to oxidize sulfite in the presence of cytochrome c as electron acceptor. ability to oxidize sulfite with ferricyanide as acceptor is undiminished, while sulfite leads to o2 activity is partially retained. gel filtration of the proteolytic products has led to the isolation of two major fragments of dissimilar size derived from sulfite oxidase. the smaller fragment has a molecular weight of 9500 and appears to be monomeri ... | 1977 | 14956 |
| porcine a blood group-specific n-acetylgalactosaminyltransferase. i. purification from porcine submaxillary glands. | the membrane-bound n-acetylgalactosaminyltransferase from porcine submaxillary glands which provides a blood group specificity to mucin has been purified 38,000-fold by affinity chromatography on udp-hesanolamine-agarose in aqueous triton x-100. design of a suitable purification procedure was developed by assessing the strength of interaction between enzyme and affinity adsorbent using batch desorption. the pure transferase has an apparent molecular weight of 100,000 as judged by zonal centrifug ... | 1977 | 14962 |
| porcine a blood group-specific n-acetylgalactosaminyltransferase. | porcine a blood group-specific n-acetylgalactosaminyl-transferase required either mn2+, cd2+, or zn2+ for activity and 2'-o-alpha-fucosylgalactosides as acceptor substrates. the presence of detergent stabilizes the enzyme but is not essential for catalysis. to obtain information about the kinetic mechanism of the transferase reaction, initial rate parameters have been determined using 2'-fucosyllactose or a--mucin as acceptors, and mn2+ or cd2+ as cosubstrates. 2'-fucosyllactose is a competitive ... | 1977 | 14963 |
| a periarterial nerve-longitudinal muscle (taenia) preparation from the guinea-pig caecum. | 1977 | 15062 | |
| possible subdivisions among alpha-adrenoreceptors in various isolated tissues. | the ratio (expressed in log10 units) of the equieffective concentrations of (+)- and (-)-noradrenaline has been measured in a variety of isolated tissues in the presence of cocaine (1 x 10(-5) m), tropolone (3 x 10(-5) m) and (+/-)-propranolol (5 x 10(-7) to 5 x 10(-5) m). the values obtained fall into 3 distinct and statistically different groups. firstly, a high group comprising (mean +/- s.e.) mouse vasdeferens (2-78 +/- 0-04), rabbit duodenum (2-91 +/- 0-07) and ileum (2-86 +/- 0-05). second ... | 1977 | 15074 |
| new selective beta2-adrenoreceptor stimulants 5(1-hydroxy-2-isopropylaminobutyl)- and 5-(1-hydroxy-2-ethylaminobutyl)-8-hydroxycarbostyril hydrochlorides (opc-2009 and opc-2030) and cyclic amp concentration. | 1977 | 15090 | |
| synthesis and adrenergic beta-blocking activity of some 1,3-benzodioxole derivatives. | a series of 1,3-benzodioxole derivatives was synthesized. we found four compounds (2,3,10 and 11 in table iv) to have about the same order of beta-blocking activity as that of sotalol. in addition, it is of interest that some of the compounds (2-4) were found to have hypotensive activites, although they were about one-tenth of that of hydralazine. sotalol did not produce any change in blood pressure, and propranolol raised the blood pressure. | 1977 | 15112 |
| beta-adrenoceptor studies. 2. effects of alkyl substitution on beta-adrenoceptor blocking, antiarrhythmic, and local anesthetic activities of 1,1'-(o-phenylenedioxy)bis(3-isopropylamino-2-propanol). | a series of bis(2-hydroxy-3-isopropylaminopropyl) ethers of nuclear-substituted catechols (1-7) has been synthesized and examined in vitro for beta-adrenoceptor blocking activity, antagonism of ouabain-induced arrhythmias, and local anesthetic activity. both tracheal and right atrial beta-adrenoceptor blocking activity are markedly decreased by alkyl substitution in position 3 of parent catechol diether 1. substitution in position 4 still lowers the affinity to cardiac arrhythmias and local anes ... | 1977 | 15113 |
| evidence against the release of prostaglandin-like material from isolated intestinal tissue by pure cholera toxin. | prostaglandin-like material was released from finely cut guinea-pig ileum or human intestinal mucosa during incubation with krebs solution. the tissue inactivated some significant change in release of prostaglandin-like material when pure cholera toxin was incubated with guinea-pig ileum or human intestinal mucosa. the work is discussed in relation to the action of cholera toxin in vivo. | 1977 | 15301 |
| purine nucleotides. | the existence of nonadrenergic, noncholinergic nerve components in the autonomic nervous system is now well established. they are strongly represented in the gastrointestinal tract of all vertebrates and have been identified in a variety of other organs, including lung, trachea, bladder, esophagus, eye, seminal vesicles, and possibly parts of the vascular and central nervous systems. their ultrastructural identification and transmission properties are known and their physiological role is beginn ... | 1976 | 15417 |
| susceptibility of various cell lines to virulent an attenuated strains of pseudorabies virus at supraoptimal temperature. | replication of virulent and attenuated strains of pseudorabies virus (prv) at supraoptimal temperature was studied in rabbit lung (zp), pig kidney (ps), and bhk-21 cells adapted and non-adapted to 40 degrees c and l, hela and human amniotic (am) cells non-adapted to 40 degrees c. the temperature of 40 degreec did not influence the type cytopathic effect (cpe) in either adapted or non-adapted cells. according to the susceptibility at 40 degrees c the cells could be divided into three groups: cell ... | 1977 | 15434 |
| glucose and lactate kinetics in burn shock. | we studied the glucose and lactate kinetics during burn shock by means of the primed constant infusion of 6-3hglucose and na-l-(+)-u-14clactate. we found an early postburn hyperglycemia that was due to an increased rate of appearance (ra) of glucose. later, the ability of the tissues to extract glucose became impaired, and the plasma glucose concentration increased even more in spite of the return of ra of glucose to the control level. the ra of lactate rose rapidly postburn, whereas the rate of ... | 1977 | 15459 |
| pig liver glyceraldehyde-3-p dehydrogenase: purification, crystallization, and characterization. | 1977 | 15520 | |
| isolation and characterization of sheep pepsin. | sheep pepsin was isolated (approx. 120-fold purification) from aqueous abomasal homogenates by (1) ph fractionation, (2) chromatography on sepharose 4b-poly-l-lysine columns and (3) gel filtration on sephadex g-100. the enzyme had mol.wt. approx. 34000, n-terminal valine and c-terminal alanine. the amino acid composition of sheep pepsin was generally similar to that of pig and ox pepsins, with a very low content of basic residues and a high content of acidic and hydroxy-amino acids. the ph optim ... | 1977 | 15546 |
| modification of pig heart lactate dehydrogenase with methyl methanethiosulphonate to produce an enzyme with altered catalytic activity. | methyl methanethiosulphonate was used to produce a modification of the essential thiol group in lactate dehydrogenase which leaves the enzyme catalytically active. methyl methanethiosulphonate produced a progressive inhibition of enzyme activity, with 2mm-pyruvate and 0.14mm-nadh as substrates, which ceased once the enzyme had lost 70-90% of its activity. in contrast, with 10mm-lactate and 0.4mm-nad+ as substrates the enzyme was virtually completely inhibited. the observed inhibition was critica ... | 1977 | 15552 |
| studies on the soluble and membrane-bound amino acid 2-naphthylamidases in pig and human epidermis. | 1. membrane-bound (particulate) and soluble amino acid 2-naphthylamidases (ec 3.5.1.-) were present in subcellular fractions of epidermis from pig and human. 2. the particulate enzymes exhibited michaelis-menten kinetics, with km 5.1x10(-5) (pig) and km 7.3x10(-5)m (human) for the substrate l-leucine 2-naphthylamide. they were inhibited by puromycin and partially inhibited by edta. they did not require heavy metals and were not inhibited by thiol-group-blocking agents. their ph optima were 7.0 ( ... | 1977 | 15553 |
| compound with bronchospasmolytical activity from the chemical class of beta-phenylethyl-aminoalkyl-xanthines (author's transl). | 1. 7-(3-2-(3,5-dihydroxyphenyl)-2-hydroxy-ethylamino-propyl)-theophylline (reproterol, d 1959, bronchospasmin) was developed as a bronchospasmolytic agent from the structure class of the phenylethyl-aminoalkyl-xanthines. 2. the pharmacological effects characterize reproterol as a bronchospasmolytic with preferential impact on the adrenergic beta2-receptors. 3. in the therapeutic dose range, reproterol stands out because of its nearly non-existent cardiovascular or central nervous side effects. e ... | 1977 | 15572 |
| purification and characterization of guinea-pig epidermal acid phosphatase. | guinea-pig epidermal acid phosphatase has been purified approximately 120-fold by a procedure including acid treatment, cm-cellulose and deae-cellulose chromatography, and gel filtration on sephadex g-100. the enzyme had a ph optimum at 5-0 and the optimal temperature for activity was approximately 50 degrees c. the enzyme was not activated by divalent cations or 2-mercaptoethanol, but it was inhibited by p-chloromercuribenzoate and by fluoride. the km value for p-nitrophenyl phosphate was 1-31x ... | 1977 | 15581 |
| evidence for a critical glutamyl and an aspartyl residue in the function of pig heart diphosphopyridine nucleotide dependent isocitrate dehydrogenase. | the ph dependence of the maximum velocity of the reaction catalyzed by diphosphopyridine nucleotide (dpn) dependent isocitrate dehydrogenase indicates the requirement for the basic form of an ionizable group in the enzyme-substrate complex with a pk of 6.6. this pk is unaltered from 10 to 33 degrees c, suggesting the ionization of a carboxyl rather than an imidazolium ion. the enzyme is inactivated upon incubation with 1-cyclohexyl-3-(2-morpholinoethyl)carbodiimide in the presence of glycinamide ... | 1977 | 15585 |
| a d-amino acid oxidase from chlorella vulgaris. | a procedure has been developed for the partial purification from chlorella vulgaris of an enzyme which catalyzes the formation of hcn from d-histidine when supplemented with peroxidase of a metal with redox properties. some properties of the enzyme are described. evidence is presented that the catalytic activity for hcn formation is associated with a capacity for catalyzing the oxidation of a wide variety of d-amino acids. with d-leucine, the best substrate for o2 consumption, 1 mol of ammonia i ... | 1977 | 15607 |
| application of trinitrophenylation for the measurement of alpha-amino residues resulting from peptic digestion. | a sensitive and precise method for the measurement of peptic activity on protein substrate is described. alpha-amino residues formed by pepsin digestion are photometrically measured by comparing the absorbances of digested and nondigested material which has been trinitrophenylated. the usual problem of high reagent-blank absorbance is eliminated by using an anion exchange resin, dowex 1-x8. in contrast to anson's method, the procedure requires only 1/100 the quantity of protein substrate for ana ... | 1977 | 15617 |
| structural glycoprotein from the media of pig aorta. aggregation of the s-carboxamidomethyl subunits. | media of pig aorta was extracted with 1 m nacl and 2 m mgcl2 to remove most of the soluble collagen, proteoglycans and glycoproteins. the glycoproteins remaining in the residue were extracted with 6 m urea-0.1 m mercaptoethanol. the urea soluble proteins were precipitated by dialysis, redissolved in 4 m guanidine-0.05 m dtt and were s-carboxamidomethylated (cm-guanidine extract). this extract was further fractionated by a variety of methods in order to separate a glycoprotein from collagen and p ... | 1977 | 15633 |
| activity of mammalian serium esterases towards malaoxon, fenitroxon and paraoxon. | 1977 | 15778 | |
| antibodies and autoantibodies of glycogen phosphorylase b: inactivation of pig and rabbit enzymes. | pig skeletal muscle glycogen phosphorylase b was purified using ammonium sulfate fractionation, deae-sephadex a-50 and sephadex g-200 column chromatography. the purified enzyme was used to immunize rabbits in the presence or in the absence of complete freund adjuvant. antibodies against pig phosphorylase in pure form were isolated from rabbit antisera using insoluble immunoadsorbents of pig phosphorylase. autoantibodies against the rabbit enzyme were obtained from the same antisera using insolub ... | 1977 | 15834 |
| phencarol--an antihistaminic preparation from the quinuclidylcarbinol group. | a high antihistaminic activity of quinuclidyl-3-diphenylcarbinol (phencarol) has been ascertained. in pharmacological experiments phencarol is superior to dimedrol (diphenhydramine), both as concerns its antihistaminic activity and the duration of its action. unlike dimedrol and diprazine (promethazine) it has no inhibitory effect on the central nervous system. with its clinical use phencarol is effective in treating urticaria, quincke's edema and allergic skin reactions. the drug is well tolera ... | 1977 | 15861 |
| derivatives of dithiocarbamic acid as inhibitors of catecholamine biosynthesis (review of the literature). | 1977 | 15863 | |
| myocardial reaction to adrenergic drugs during the early periods of development (review of the literature). | 1977 | 15864 | |
| pure renin. isolation from hog kidney and characterization. | the pressor enzyme renin (ec 3.4.99.19) was isolated in a pure and stable form from hog kidney by affinity chromatography on a pepstatin/agarose gel followed by three additional steps of conventional chromatography. destruction of the enzyme by proteolysis during isolation was prevented by chemically eliminating proteases in extracts. the pure preparation was used for the characterization of this enzyme. renin was found to be a glycoprotein containing glucosamine and possessing binding affinity ... | 1977 | 16012 |
| solubilization and properties of mannose and n-acetylglucosamine transferases involved in formation of polyprenyl-sugar intermediates. | a particulate fraction from porcine aorta catalyzed the incorporation of n-acetylglucosamine (glcnac) from udp-3hglcnac into both glcnac-pyrophosphorylpolyprenol and glcnac-glcnac-pyrophosphorylpolyprenol. this transfer utilized endogenous lipid and required a divalent cation. mn2+ was the best metal ion and was optimum at 2.3 mm. this same particulate fraction was previously shown to transfer mannose from gdp-14cmannose to endogenous lipid to form mannosylphosphorylpolyprenol (chambers, j., and ... | 1977 | 16014 |
| bile acid ethyl esters. their infrequent formation during routine bile acid analysis and identification by gas chromatography and mass spectrometry. | 1977 | 16027 | |
| energy phosphate turnover and glycolysis in skeletal muscle of the pietrain pig: the effects of premedication with azaperone and pentobarbitone anaesthesia. | 1977 | 16042 | |
| epidermal nucleases. ii. the multiplicity of ribonucleases in guinea-pig epidermis. | ribonuclease activity has been extracted from adult guinea-pig epidermis by sequential homogenization in dilute sodium acetate and sulfuric acid. the extracts were subjected to ammonium sulfate fractionation and to affinity and ion exchange chromatography. three ribonucleases (i, ii, iii) were separated from the sodium acetate extract and 6(a, b1, b2, b3, c, d) were isolated from the sulfuric acid extract. the degree of purification varies from 65-fold to 8,700-fold and the apparent molecular we ... | 1977 | 16063 |
| hog cerebellar d-amino acid oxidase and its histochemical and immunofluorescent localization. | 1977 | 16084 | |
| intestinal brush-border sucrase: ph-dependent effects of harmaline and alkali metal ions proceedings. | 1977 | 16127 | |
| effect of cardiovascular drugs on electrolyte metabolism (review of the literature). | 1977 | 16403 | |
| nitrogen and amino acid content in sow's milk at different stages of lactation. | 1977 | 16414 | |
| complex of d-glyceraldehyde-3-phosphate dehydrogenase with cu2+ ion. the properties of ternary cu-enzyme-coenzyme complex. | the formation of ternary cu-enzyme-coenzyme complex from cupric ion and d-glyceraldehyde-3-phosphate dehydrogenase holoenzyme results in similar spectral changes as the formation of binary cu-apoenzyme complex, which indicates that the complex bonds between cupric ion and the holoenzyme, and cupric ion and the apoenzyme are similar. spectrophotometric titration, chemical modification experiments and inhibition studies with cupric ion gave evidence that cupric ion is selectively bound on cys-149 ... | 1976 | 16425 |
| the splitting of sperm heads from tails in eight mammalian species and the measurement of their sizes. | ejaculated spermatozoa from man, the euopean wild boar and the bull, and spermatozoa from the cauda epididymes of the rabbit, rat, mouse, hamster and guinea pig were treated with a sonic bath, a sonic probe, trypsin with and without prior treatment with a sulphhydryl reagent, pronase and alkalis. the fragments produced were counted and sized in an accurately calibrated coulter counter, model zb industrial, before and after zaponin treatment to lyse accompanying debris and the peripheral cytoplas ... | 1977 | 16511 |
| activation of cerebral guanylate cyclase by nitric oxide. | 1977 | 16602 | |
| (na+ + k+)atpase inhibition by palythoa extracts--chemical nature of the inhibitor and kinetics of inhibition. | 1977 | 16609 | |
| calcium binding site of trypsin as probed by lanthanides. | 1977 | 16646 |