Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| acute hypotensive responses to peptide inhibitors of renin in conscious monkeys: an effect on blood pressure independent of plasma renin inhibition. | in order to investigate the hypotensive mechanisms of action of peptide renin inhibitors, blood pressure responses to five renin inhibitors were compared with those to the angiotensin converting enzyme inhibitor, enalaprilat, in conscious african green and rhesus monkeys. (3s-4s)-4-amino-5-cyclohexyl-3-hydroxy pentanoic acid (achpa)-containing renin inhibitory peptide (acrip) and enalaprilat both decreased blood pressure in euvolemic and volume-depleted african green monkeys. however, while a ma ... | 1990 | 2159506 |
| experimental infection of african green monkeys and cynomolgus monkeys with a sivagm strain isolated from a healthy african green monkey. | an infection occurred in all african green monkeys and cynomolgus monkeys experimentally inoculated with sivagm [tyo-1], as demonstrated by the appearance of an antibody to sivagm [tyo-1] and the isolation of the virus. no monkey exhibited overt clinical disorders throughout the experimental period of 42 weeks. thus, sivagm was not pathogenic to its original host or to macaques. this system is proposed as a model for hiv infection manifesting no overt disease. | 1990 | 2160015 |
| the growth of simian virus 40 (sv40) host range/adenovirus helper function mutants in an african green monkey cell line that constitutively expresses the sv40 agnoprotein. | the simian virus 40 t-antigen carboxy-terminal mutants, dla2459 and dla2475, are cell line and temperature dependent for growth and plaque formation in monkey kidney cells. although these mutants did form plaques on bsc-1 cells at 37 degrees c, they were about fivefold less efficient for plaque formation than wild-type simian virus 40. these mutants did not grow in cv-1 cells and did not synthesize agnoprotein in those cells. cv-1 cells which constitutively express the agnoprotein were permissiv ... | 1990 | 2161954 |
| prostaglandins mediate the ocular hypotensive action of the angiotensin converting enzyme inhibitor mk-422 (enalaprilat) in african green monkeys. | mk-422 (enalaprilat) (0.0005-0.5%) significantly reduced intraocular pressure (iop) in african green monkeys. studies utilizing unilateral instillation of mk-422 and its inactive r-isomer indicated a local site of action within the eye which is dependent upon inhibition of angiotensin converting enzyme, also known as kininase ii. tonography showed a small increase (21%) in conventional aqueous humor outflow facility which did not entirely account for the iop lowering effect of mk-422. pretreatme ... | 1990 | 2163428 |
| occurrence of nonspecific reactions among stool specimens tested by the abbott testpack rotavirus enzyme immunoassay. | sixty-five stool specimens obtained from children suffering from gastroenteritis were tested for the presence of antigen to rotavirus by the abbott testpack rotavirus (testpack) enzyme immunoassay kit. the kallestad pathfinder enzyme immunoassay, polyacrylamide gel electrophoresis, immune electron microscopy, and virus isolation were utilized as reference assays. fifty-four specimens were in accord by testpack and kallestad pathfinder. among 11 discordant specimens positive with testpack but neg ... | 1990 | 2166074 |
| inhibition of simian varicella virus infection of monkeys by 1-(2-deoxy-2- fluoro-1-beta-d-arabinofuranosyl)-5-ethyluracil (feau) and synergistic effects of combination with human recombinant interferon-beta. | 1-(2-deoxy-2-fluoro-1-beta-d-arabinofuranosyl)-5-ethyluracil (feau) has been shown to be a highly effective inhibitor of simian varicella virus infection in african green monkeys. administration of feau by either intravenous injection or gavage at doses as low as 1 mg/kg/day prevented the development of rash and reduced viremia. the effective dose could be further reduced to 0.2 mg/kg/day when administered in combination with a sub-effective dose of human recombinant interferon-beta. no evidence ... | 1990 | 2166466 |
| a comparison of bkv, jcv, and sv 40 transcriptional enhancers in primate cells. application of the two-phase partition assay for chloramphenicol acetyl transferase (cat). | the activities of the transcriptional enhancers of the prototype human polyomaviruses, bkv (dun) and jcv (mad-1), were compared with that of the rhesus virus sv 40 in old world african green monkey kidney cells (cv-1) and in new world owl monkey kidney cells (omk). enhancer activities were tested in cat-expression plasmids. cat activity in transfected cell extracts were measured by the novel two-phase partition assay. the activities of the bkv and jcv enhancers were 45% and 20% that of sv 40, re ... | 1990 | 2171455 |
| [a karyotype study of the cells of the african green monkey kidney cell line 4647 cultured long term in media with different sera]. | the karyological analysis of the cell line 4647 used for production of a killed vaccine to hepatitis a virus was run in the 98th, 107th, 117th and 127th passages by the routine and c, g, and ag methods of differential chromosome staining. a considerable balancing of the chromosome composition at 107-127 passage levels is shown. the cells of line 4647 present a significant heterogeneity, as to the number of chromosomes, and do not belong to any distinct modal class. the modal number of chromosome ... | 1990 | 2175469 |
| the mechanism of the antiherpetic activity of zinc sulphate. | the molecular mechanism of the effects of zinc ions against herpes simplex virus (hsv) infection was investigated. zinc sulphate (100 microm) in the culture medium of an hsv-infected african green monkey kidney cell line did not block viral dna synthesis and, at this concentration, only moderate cytotoxic effects were observed in uninfected cells. nevertheless, virus yields were reduced to less than 1% of the control. thus the long standing hypothesis that zinc might block multiplication of hsv ... | 1990 | 2177090 |
| [herpes simplex type i virus observed in the superior cervical ganglion from human cadavers and autopsy materials]. | herpes simplex type i (hsv i) causes some infections such as herpes labialis, encephalitis, keratoconjunctivitis and also some cranial nerve syndromes such as acute vestibular neuritis, migraine and meniere's disease in human. we used 4 fixated and 16 fresh cadavers to isolate hsv i virus from the superior cervical ganglia. the ganglia materials are inoculated to prk (primary rabbit kidney), vero (african green monkey kidney) and bhk 21 (baby hamster kidney) cell lines in order to isolate the vi ... | 1990 | 2178213 |
| complementation of the rev gene mutation among human and simian lentiviruses. | the functional exchangeability of the rev gene was assessed in transient transfection experiments by using in vitro-constructed rev and gag mutants of the following three primate lentiviruses: human immunodeficiency virus type 1 (hiv-1), hiv-2, and simian immunodeficiency virus from the african green monkey (siv agm). cotransfection into sw480 cells of the rev and gag mutants derived from the dna of each infectious virus resulted in the generation of progeny particles as determined by reverse tr ... | 1990 | 2182909 |
| human immunodeficiency virus-like, nonreplicating, gag-env particles assemble in a recombinant vaccinia virus expression system. | we report the assembly of human immunodeficiency virus (hiv)-like particles in african green monkey kidney cells coinfected with two recombinant vaccinia viruses, one carrying the hiv-1 gag and protease genes and the other the env gene. biochemical analysis of particles sedimented from culture supernatants of doubly infected cells revealed that they were composed of gag proteins, primarily p24, as well as the env proteins gp120 and gp41. thin-section immunoelectron microscopy showed that these p ... | 1990 | 2186175 |
| genetic organization of a chimpanzee lentivirus related to hiv-1. | simian immunodeficiency viruses have been isolated from four species of monkey, the 'captive' macaque and mangabey and the 'feral' african green monkey and mandrill. while none of these viruses is a replica of hiv-1, the macaque and mangabey viruses represent correct genetic models for hiv-2, possessing exactly the same complement of genes. recently a lentivirus has been identified in two wild chimpanzees (pan troglodytes troglodytes) in gabon, west equatorial africa, and isolated from one of th ... | 1990 | 2188136 |
| primary sequence and heterologous expression of nuclear pore glycoprotein p62. | the major nuclear pore protein p62 is modified by o-linked n-acetylglucosamine and functions in nuclear transport. we have cloned, sequenced, and expressed the full-length rat p62 cdna. the rat p62 mrna is 2,941 nucleotides long and encodes a protein of 525 amino acids containing 30% serine and threonine residues. the amino acid sequence near the amino-terminus contains unique tetrapeptide repeats while the carboxy-terminus consists of a series of predicted alpha-helical regions with hydrophobic ... | 1990 | 2190987 |
| hepatic apo b-100 lipoproteins and plasma ldl heterogeneity in african green monkeys. | the contribution of hepatic apolipoprotein (apo) b-100 lipoproteins to plasma low-density lipoprotein (ldl) metabolic heterogeneity was examined in african green monkeys. hepatic 3h-labeled very low-density lipoproteins (vldl) (d less than 1.006, where d is density in g/ml) or hepatic 131i-labeled ldl (1.030 less than d less than 1.063) were isolated from perfused livers and injected simultaneously with autologous plasma 125i-ldl into african green monkeys. serial blood samples were taken, and t ... | 1990 | 2193530 |
| comparison of three enzyme immunoassays to tissue culture for the diagnosis of rotavirus gastroenteritis in infants and young children. | three enzyme immunoassays (eias), rotazyme ii, idl, and pathfinder, were evaluated for rotavirus detection in stool and rectal swab specimens from children with symptomatic gastroenteritis and compared with virus isolation in primary african green monkey kidney cells. of 125 specimens tested, 49 were rotavirus positive by tissue culture isolation; of these 49, 40 were positive by rotazyme ii, 43 were positive by idl, and 46 were positive by pathfinder eias. as compared with tissue culture isolat ... | 1990 | 2199504 |
| cognitive and motor deficits in the performance of an object retrieval task with a barrier-detour in monkeys (cercopithecus aethiops sabaeus) treated with mptp: long-term performance and effect of transparency of the barrier. | to assess the stability of neural deficits produced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (mptp), the performance of monkeys on an object retrieval (detour) task was studied. the task required retrieval of a banana slice from a transparent box open on one side and fastened to a tray in front of the cage. the orientation of the open side, position on the tray, and position of the banana in the box were manipulated to vary the difficulty of the trials. six african green monkeys (cercopit ... | 1990 | 2206426 |
| intraocular pressure changes and postural changes of intraocular pressure in experimentally induced hansen's disease of rhesus, mangabey, and african green monkeys. | in our long term evaluation of patients with hansen's disease we have frequently found reduction of their intraocular pressure. furthermore, we noted changes in their intraocular pressure on change of posture. to determine if these changes have any significance we measured the intraocular pressures of 24 experimentally infected and 39 control monkeys in both sitting and reclining positions. we found significant reduction of intraocular pressure in 66.7% compared with controls in the sitting posi ... | 1990 | 2223699 |
| identification and characterization of a novel mammalian intermediate filament-associated protein. | a novel monoclonal antibody, designated m1.4, recognizes the high molecular weight microtubule-associated protein map1a (ca. mr 380 kd) in both bovine and rat brain. in hela cells, however, m1.4 binds to a 240 kd polypeptide on immunoblots and co-localizes with both vimentin and cytokeratin filaments using double-label immunofluorescence microscopy. immunoelectron microscopy indicates that the 240 kd polypeptide localizes along bundled intermediate filaments in a periodic manner. two-dimensional ... | 1990 | 2225087 |
| cytotoxic and hemolytic effects of tritrichomonas foetus on mammalian cells. | geographically distinct lines of tritrichomonas foetus were assayed for their ability to cause cytotoxicity in nucleated mammalian cells and lysis of bovine erythrocytes. t. foetus was highly cytotoxic toward a human cervical cell line (hela) and early bovine lymphosarcoma (bl-3) but displayed low levels of cytotoxicity against african green monkey kidney (vero) cells. in addition to variation in the extent of cytotoxicity toward different targets, differences in the levels of cytotoxicity in th ... | 1990 | 2228233 |
| mutational analysis of simian immunodeficiency virus from african green monkeys and human immunodeficiency virus type 2. | we constructed ten mutants of simian immunodeficiency virus isolated from african green monkey (sivagm), and nine mutants of human immunodeficiency virus type 2 (hiv-2) in vitro. their infectivity, cytopathogenicity, transactivation potential, virus rna, and protein synthesis were examined by transfection and infection experiments. mutations in three structural (gag, pol, env) and two regulator (tat, rev) genes abolished the infectivity of both viruses, but vpx, vpr (hiv-2), and nef were dispens ... | 1990 | 2231682 |
| molecular characterization of simian lentiviruses from east african green monkeys. | asymptomatic infection with simian lentiviruses (also called simian immunodeficiency viruses, or siv) is common among feral african green monkeys. to characterize the range of siv genetic diversity among infected african green monkeys, we have determined nucleotide sequences from complete or partial molecular clones of four distinct sivagm isolates from kenya and ethiopia. the nucleotide and amino acid variability we observed among the sivagm isolates was greater than the variability within any ... | 1990 | 2231686 |
| effect of dietary fish oil on coronary artery and aortic atherosclerosis in african green monkeys. | studies were carried out for 2.5 to 3 years in adult male african green monkeys (grivet subspecies) fed diets containing 22% of calories as lard or fish oil with 40% of calories as fat and 0.75 mg cholesterol/kcal to determine if isocaloric substitution of menhaden fish oil for lard affects coronary artery atherosclerosis. the average total plasma cholesterol concentrations during the experimental period were significantly lower for the fish-oil group (231 +/- 37 mg/dl) compared to the lard grou ... | 1990 | 2244858 |
| immunological studies of the basis for the apathogenicity of simian immunodeficiency virus from african green monkeys. | potential reasons for the lack of pathogenicity of the simian immunodeficiency virus sivagm in its natural host, the african green monkey (agm, cercopithecus aethiops), were investigated with respect to immunological mechanisms. the functional immune response of monkeys to infection was similar (though not identical) to that of humans to infection with human immunodeficiency virus type 1 (hiv-1). in the sera of infected animals, neutralizing antibodies were found to be low or absent, and in part ... | 1990 | 2247482 |
| effects of speed on forelimb joint angular displacement patterns in vervet monkeys (cercopithecus aethiops). | shoulder, elbow and wrist joint angular displacement patterns were analyzed for five vervet monkeys across increasing speed. within symmetrical gaits, the peak positions of the pattern for each joint tended to decrease with increasing speed as did the yield angle of the elbow (more "yielding"). across the walk(run)-gallop transition there were no notable changes in the displacement patterns, but there was a consistent decrease in the range of elbow movements and an increase in the yield angle. a ... | 1990 | 2248379 |
| the representation of social relations by monkeys. | monkeys recognize the social relations that exist among others in their group. they know who associates with whom, for example, and other animals' relative dominance ranks. in addition, monkeys appear to compare types of social relations and make same/different judgments about them. in captivity, longtailed macaques (macaca fascicularis) trained to recognize the relation between one adult female and her offspring can identify the same relation among other mother-offspring pairs, and distinguish ... | 1990 | 2269006 |
| african green monkeys have sexually dimorphic and estrogen-sensitive hypothalamic neuronal membranes. | previous studies have shown sex differences in intramembrane particle content in the arcuate neurons of the rat hypothalamus. in this study, freeze-fracture replicas were prepared from the infundibular hypothalamus of adult african green monkeys (cercopithecus aethiops) in order to determine whether primates also have sexual dimorphism in neuronal membranes. intramembrane particles (imp) were quantitatively assessed in the perikaryal plasma membranes of infundibular neurons. four groups of monke ... | 1990 | 2271961 |
| effect of fish oil on atherosclerosis and lipoprotein metabolism. | this review primarily covers work on the effects of dietary n-3 fatty acids on lipoprotein metabolism and atherosclerosis that has been done in a nonhuman primate model, the african green monkey, and puts it in context with work of others using humans and other experimental animals. detection of effects of n-3 fatty acids in the monkey model was facilitated by a considerable enrichment of dietary fat with fish oil (about 20% of dietary calories came from menhaden oil in the fish oil group or abo ... | 1990 | 2282103 |
| construction and characterization of an infectious dna clone and of mutants of simian immunodeficiency virus isolated from the african green monkey. | we constructed a full-length molecular clone of simian immunodeficiency virus from an african green monkey. upon transfection, this clone directed the production of virus particles cytopathic and infectious to human cd4+ leukemia cell lines. mutations were introduced by recombinant dna techniques into eight open reading frames of simian immunodeficiency virus from the african green monkey thus far identified. the phenotypes of mutant viruses, i.e., infectivity, cytopathogenicity, transactivation ... | 1990 | 2293665 |
| recombination between irradiated shuttle vector dna and chromosomal dna in african green monkey kidney cells. | an autonomously replicating shuttle vector was used to investigate enhancement of plasmid-chromosome recombination in mammalian host cells by gamma irradiation and uv light. sequences homologous to the shuttle vector were stably inserted into the genome of african green monkey kidney cells to act as the target substrate for these recombination events. the shuttle vector molecules were irradiated at various doses before transfection into the mammalian host cells that contained the stable insertio ... | 1990 | 2294408 |
| mutational analysis of the human immunodeficiency virus type 2 (hiv-2) genome in relation to hiv-1 and simian immunodeficiency virus siv (agm). | we constructed an infectious molecular clone of the human immunodeficiency virus type 2 (hiv-2) and generated nine frameshift mutants corresponding to nine open reading frames identified so far. three structural (gag, pol, env) and two regulative (tat, rev) gene mutants were not infectious, whereas vif, vpx, vpr, and nef genes were dispensable for infectivity. all of the mutants except env and rev were cytopathic in cd4+ human leukemia cells. in transfection assays, the expression of hiv-2 long ... | 1990 | 2296082 |
| [a method for collecting semen by fingers in the african green monkey (cercopithecus aethiops) and properties of the semen collected]. | we tried to make a successful collection of semen by human fingers, using ten african green monkeys (cercopithecus aethiops) aged from 5-7 years, which were bred and reared at tsukuba primate center for medical science. under anesthesia, the animals were laid side way and their penis were gently stimulated by fingers. semen was successfully obtained from five animals aged 6-7 years, but it was impossible to collect semen from the remaining five animals aged 5 years. it took only 5-15 seconds fro ... | 1990 | 2303088 |
| simian immunodeficiency viruses from african green monkeys display unusual genetic diversity. | african green monkeys are asymptomatic carriers of simian immunodeficiency viruses (siv), commonly called sivagm. as many as 50% of african green monkeys in the wild may be siv seropositive. this high seroprevalence rate and the potential for genetic variation of lentiviruses suggested to us that african green monkeys may harbor widely differing genotypes of sivagm. to investigate this hypothesis, we determined the entire nucleotide sequence of an infectious proviral molecular clone of sivagm (1 ... | 1990 | 2304139 |
| the thromboxane receptor antagonist sq 30,741 reduces myocardial infarct size in monkeys when given during reperfusion at a threshold dose for improving reflow during thrombolysis. | the threshold dose of the selective thromboxane receptor antagonist sq 30,741 for increasing reflow during thrombolysis was identified and then evaluated in a model of myocardial ischemia with reperfusion. in anesthetized cynomolgus monkeys, stenotic carotid arteries were occluded with a platelet-rich thrombus by electrical stimulation and recanalized with streptokinase (680 u/min intraarterially for 1 h) and heparin (200 u/kg + 120 u/h intravenously for 3 h). concurrent administration of sq 30, ... | 1990 | 2307799 |
| enhancement of siv infection with soluble receptor molecules. | the cd4 receptor on human t cells has been shown to play an integral part in the human immunodeficiency virus type 1 (hiv-1) infection process. recombinant soluble human cd4 (rcd4) was tested for its ability to inhibit sivagm, an hiv-like virus that naturally infects african green monkeys, in order to define t cell surface receptors critical for sivagm infection. the rcd4 was found to enhance sivagm infection of a human t cell line by as much as 18-fold, whereas hiv-1 infection was blocked by rc ... | 1990 | 2309120 |
| effects of size on vervet (cercopithecus aethiops) gait parameters: a longitudinal approach. | changes in the values of certain locomotor parameters were analyzed over a range of speeds for five immature vervet monkeys sampled at 6 month intervals over approximately a 3 year period. lateral and diagonal sequence walking gaits and transverse and rotary gallops were commonly used. the monkeys switched from walking to galloping at long cycle durations for their mass, although, as a group, their transition speeds were in agreement with data from other mammals. however, for individual monkeys, ... | 1990 | 2327483 |
| effects of growth and speed on hindlimb joint angular displacement patterns in vervet monkeys (cercopithecus aethiops). | hip, knee, and ankle joint displacement patterns are compared across both age and speed for five immature vervet monkeys sampled approximately every 6 months over a 3 year period. the analysis indicated that, as a group, the animals displayed no consistent changes in joint patterns as they grew. however, individual animals showed consistent patterns. there were also no consistent effects of size across animals at the walk-gallop transition. this is contrary to mcmahon's prediction (j. appl. phys ... | 1990 | 2327484 |
| synergism through direct covalent bonding between agents: a strategy for rational design of chemotherapeutic combinations. | self-assembling chemotherapeutic agents are mixtures of relatively nontoxic precursors that can combine chemically under physiological conditions to form products with greater cytotoxic and/or antimicrobial activity than either of the precursors. combinations that form products more rapidly in or near the target (tumor, pathogen, virally infected cell) than in normal tissues will exhibit target-selective synergism, thus exhibiting an antitarget selectivity that is greater than the selectivities ... | 1990 | 2328289 |
| isolation and characterization of simian immunodeficiency viruses from two subspecies of african green monkeys. | cercopithecus aethiops (african green monkey), a nonhuman primate species distributed throughout subsaharan africa, has been shown to have high seroprevalence rates of antibodies to simian immunodeficiency virus (siv), and therefore, has been proposed as a natural reservoir for immunodeficiency viruses. our laboratories have isolated siv-like viruses from two east african subspecies of c. aethiops designated grivet and vervet monkeys. analysis of the structural proteins based on the molecular we ... | 1990 | 2340199 |
| effect of fish oil diet on hepatic lipid metabolism in nonhuman primates: lowering of secretion of hepatic triglyceride but not apob. | african green monkeys were fed diets containing either 11% (by weight) fish oil or lard for 2.5 yr. to test the hypothesis that fish oil decreases hepatic secretion of triglyceride (tg) and apob, livers from these animals were perfused with a fatty acid mixture [85% (w/w) oleate containing [14c]oleate and 15% n-3 containing [3h]eicosapentaenoic acid (epa)] at a rate of 0.1 mumol fatty acid/min per g liver. liver perfusate was sampled every 30 min during 4 h of recirculating perfusion. the concen ... | 1990 | 2341808 |
| species, diet, and gender differences in plasma postheparin lipolytic activities in nonhuman primates. relationships with plasma lipids and high density lipoproteins. | studies were done in selected groups of nonhuman primates in which the diet (low fat and high fat as either polyunsaturated or saturated fat), gender, and species (african green monkeys and cynomolgus monkeys) were varied. large differences in plasma lipid and lipoprotein concentrations were found among these groups, providing the opportunity to examine their relationships to postheparin lipolytic activities (phla) including lipoprotein lipase (lpl) and hepatic triglyceride lipase (htgl). phla w ... | 1990 | 2344294 |
| physiology and function of the vero cell receptor for the hepatitis b virus small s protein. | the african green monkey kidney-derived vero cell line expresses a receptor activity for noninfectious hepatitis b surface antigen (hbsag) particles containing the small s protein. (m.e. peeples, k. komai, r. radek, and m.j. bankowski, 1987, virology 160, 135-142). in this report, the binding characteristics, the physiological requirements, and the functions of this receptor are further characterized. the association rate constant (ka) was determined by measuring binding during a short (10-min) ... | 1990 | 2353459 |
| acoustic classification of alarm calls by vervet monkeys (cercopithecus aethiops) and humans (homo sapiens): i. natural calls. | a 2-choice, operant-conditioning-based classification procedure was developed in which vervet monkeys (cercopithecus aethiops) categorized species-typical snake and eagle alarm calls recorded from individually identified free-ranging animals. after preliminary training with a pair of calls from a single animal, 2 vervets were tested with novel exemplars produced by a variety of callers. experiment 1 combined testing with continued training in routine classification of 14 new calls. in experiment ... | 1990 | 2354626 |
| acoustic classification of alarm calls by vervet monkeys (cercopithecus aethiops) and humans (homo sapiens): ii. synthetic calls. | in 2 experiments classification of synthetic versions of species-typical snake and eagle alarm calls by vervet monkeys (cercopithecus aethiops) and human (homo sapiens) control subjects was investigated. in a 2-choice, operant-conditioning-based procedure, this work followed up acoustic analyses that had used various digitally based algorithms (owren & bernacki, 1988). all subjects were first tested with alarm-call replicas that were based on analysis data. these models were classified in the sa ... | 1990 | 2354627 |
| voluntary consumption of beverage alcohol by vervet monkeys: population screening, descriptive behavior and biochemical measures. | seventeen percent of 196 feral vervet monkeys (cercopithecus aethiops) spontaneously drank appreciable quantities of beverage alcohol in 3% sucrose in preference to 3% sucrose alone. ethanol consumption increased over time, as did the concentration of ethanol tolerated. willingness to select ethanol was stable over a three-year period, as measured by periodic retesting. individual patterns of drinking and behavioral responses to ethanol were quite variable. upon occasion, some animals drank to a ... | 1990 | 2356209 |
| cognitive and motor deficits in the acquisition of an object retrieval/detour task in mptp-treated monkeys. | to assess functional changes following treatment with 1-methyl-4-phenyl- 1,2,3,6-tetrahydropyridine (mptp) in monkeys, we studied a task that reveals sensitivity to dopamine deficits under various conditions. the task required retrieval of a banana slice from a transparent box that is open on one side and fastened to a tray. successful performance required the subject to suppress a tendency to reach directly at the reward while (1) orientation of the open side, (2) position on the tray, and (3) ... | 1990 | 2364263 |
| mammalian cell cultures affected by naegleria gruberi. | amebae of 8 strains of naegleria gruberi were able to destroy 10 established mammalian cell lines including lung, kidney, ovary, connective tissue, neuroblastoma, and laryngeal and cervical carcinoma cells. the strains of n. gruberi varied in their ability to produce a destructive effect (de) in african green monkey kidney (vero) cell cultures. however, cell line susceptibility was found to be equivalent when tested with the considerably destructive 1518/l strain of n. gruberi. the vero cell lin ... | 1990 | 2370024 |
| [the induction of specific dicentric chromosomes in cho-6 chinese hamster ovary cells]. | under a long treatment of cells cho-6 with 5-bromodeoxyuridine and colcemid it was discovered that dicentric chromosomes in metaphases of the first division of polykaryocytes contained micronuclei. when the cells were treated with colcemid only, the number of dicentrics was less. this phenomenon was described earlier in the chinese hamster cell line b11d-ii faf28. under the similar testing of continuous cell lines of human, swine, african green monkey and syrian hamster origin this result failed ... | 1990 | 2375062 |
| experimental transmission of leishmania major to vervet monkeys (cercopithecus aethiops) by bites of phlebotomus duboscqi (diptera: psychodidae). | experimental transmission of leishmania major to vervet monkeys (cercopithecus aethiops) was accomplished by bites of phlebotomus duboscqi sandflies. three-day-old, laboratory-reared p. duboscqi were fed on leishmanial lesions on hamsters infected with l. major. the flies were re-fed on monkeys 10 d after infection. five adult male vervet monkeys were used in concurrent transmission trials. two of the monkeys received subcutaneous inoculations with stationary-phase promastigotes (2 x 10(6) proma ... | 1990 | 2389312 |
| [growth curves of body weight and their relationship to sexual maturity in laboratory-bred male african green monkeys (cercopithecus aethiops)]. | nonlinear growth models having a three- or four-parameter family were applied to individual body weight data of 5 male african green monkeys for estimating their growth patterns. body weight was measured from birth to six years of age and 58 to 114 data items per monkey were collected. the average body weight at birth was 360g with the standard deviation of +/- 25g, 4.54 +/- 0.29 kg at five years of age, and 4.50 +/- 0.12 kg at six years of age at which point body weight was judged to have reach ... | 1990 | 2401318 |
| parvovirus-like particles associated with diarrhea in unweaned piglets. | numerous parvovirus-like particles, 18 to 26 nm in diameter, were detected by electron microscopy in the intestinal contents of two to three week old piglets with mild to severe diarrhea, in six quebec pig herds. hemagglutination of guinea pig and african green monkey red blood cells was obtained with clarified intestinal contents. two isolates were found to be antigenically related to porcine and canine parvoviruses, while another differed from the porcine parvovirus using the hemagglutination- ... | 1985 | 2412678 |
| s-phase patterns of cyclin (pcna) antigen staining resemble topographical patterns of dna synthesis. a role for cyclin in dna replication? | the sequence of cyclin (proliferating cell nuclear antigen, pcna), antigen staining throughout the cell cycle of african green monkey kidney cells (bs-c-1) has been determined by indirect immunofluorescence using pcna autoantibodies specific for this protein. patterns of cyclin staining observed between the beginning of s-phase and maximum dna synthesis are similar to those reported in human ama cells [(1985) proc. natl. acad. sci. usa 82, 3262-3266], while those detected thereafter are signific ... | 1985 | 2415394 |
| improved in situ hybridization and g-banding by pretreatment with denhardt's solution and gelatin-chrome alum. | various pretreatments of metaphase spreads were examined to obtain optimal dna labelling patterns while maintaining chromosome integrity during in situ hybridization procedures. preparations of african green monkey (agm) chromosomes fixed in methanol-acetic acid (cv-1 cell line) were treated by coating with denhardt's solution, dilute gelatin-chrome alum, nonfat instant dry milk dissolved in saline-citrate solution (ssc) and/or acetylation prior to denaturation of chromosomal dna in 70% formamid ... | 1985 | 2417994 |
| effects of chronic fenfluramine on blood serotonin, cerebrospinal fluid metabolites, and behavior in monkeys. | the effects of long term (70 days) fenfluramine treatment on selected physiological and behavioral measures were examined in four adult male vervet monkeys (cercopithecus aethiops sabaeus). relative to pretreatment baseline values, whole blood serotonin (wbs) and cerebrospinal fluid 5-hydroxyindole acetic acid (5-hiaa) were reduced, cerebrospinal fluid homovanillic acid (hva) was unaltered, and aggressive and locomotor behavior were increased. both physiological and behavioral effects were rever ... | 1986 | 2433707 |
| serological survey and virus isolation of simian t-cell leukemia/t-lymphotropic virus type i (stlv-i) in non-human primates in their native countries. | infection with a simian retrovirus (stlv-i) closely related to human t-lymphotropic virus type i (htlv-i) was investigated in non-human primates living in their native countries in africa and asia. serum antibodies cross-reacting with htlv-i antigens were detected in 85 of 567 non-human primates of 30 species. seropositive animals were found among african green monkeys, olive baboons, sykes' monkeys, mandrills and patas monkeys in several countries in africa, and cynomolgus monkeys, celebes maca ... | 1987 | 2440820 |
| isolation of simian immunodeficiency virus from african green monkeys and seroepidemiologic survey of the virus in various non-human primates. | sixteen isolates of simian retrovirus closely related to human immunodeficiency virus (hiv) were obtained from healthy african green monkeys (agm) (cercopithecus aethiops). the first isolate was obtained from a monkey seropositive for hiv, and the others were isolated from monkeys harboring antibodies to the first isolate. these simian retroviruses were referred to as simian immunodeficiency virus from agm, siv[agm], due to their cross-reactivities with hiv structural proteins. these siv[agm] is ... | 1988 | 2447023 |
| rubella virus replication: effect of interferons and actinomycin d. | the effect of alpha and gamma interferon (ifn alpha, ifn gamma) and actinomycin d on the expression of wild type rubella virus in african green monkey kidney cells (vero 76) was studied. viral protein synthesis in the infected cells was significantly reduced upon treatment of the cells with ifn alpha or ifn gamma, which is accompanied by the reduction in the level of both the (+) stranded and the (-) stranded viral rnas. the residual rubella viral rna from interferon-treated cells, however, was ... | 1988 | 2453976 |
| production and characterization of monoclonal antibodies specific for the transmembrane protein of simian immunodeficiency virus from the african green monkey. | mouse monoclonal antibodies were produced against simian immunodeficiency virus (siv) from the african green monkey (sivagm). the antibodies reacted with the transmembrane protein of all five sivagm isolates but not with those of sivs from the rhesus macaque and mandrill or of human immunodeficiency virus type 1 or type 2, indicating that they recognize a species-specific epitope strongly conserved in sivagm. the transmembrane proteins of several sivagm isolates were found to vary in molecular s ... | 1988 | 2460641 |
| amino acid substitution at position 226 of the hemagglutinin molecule of influenza (h1n1) virus affects receptor binding activity but not fusion activity. | the receptor binding site of the hemagglutinin (ha) molecule of type a influenza virus a/ussr/90/77 (h1n1) has been studied. site-specific mutagenesis has been used to introduce base changes into the sequence that codes for the amino acid residue at position 226 on the ha molecule, and mutant sequences replaced the wild-type sequence of the ha gene of the sv40-ha recombinant virus (svha). mutant ha proteins were expressed in african green monkey kidney cells and analyzed for receptor binding and ... | 1988 | 2460997 |
| extensive genetic variability of simian immunodeficiency virus from african green monkeys. | serological surveys have revealed that 30 to 50% of wild-caught african green monkeys have antibodies reactive to simian immunodeficiency virus (siv), a retrovirus related to human immunodeficiency virus (hiv). although the nucleotide sequence of one sivagm isolate, tyo1, was recently reported, the extent of genetic variability among sivagm isolates remains to be determined. restriction endonuclease mapping of infectious molecular clones of two sivagm isolates (266 and 385), described in this no ... | 1989 | 2467010 |
| isolation of human immunodeficiency virus-related simian immunodeficiency viruses from african green monkeys. | we have isolated lentivirus strains that are related to the human immunodeficiency virus (hiv) from african green monkeys (cercopithecus aethiops; agm). although immunologically related, these sivagm are clearly distinct from other simian immunodeficiency virus (siv) isolates, including isolates from macaca mulatta (sivmac) or even from other agm. the sivagm strains described in this communication grow well in a limited number of human t-lymphoma lines. virus density, morphology, and reverse tra ... | 1989 | 2468160 |
| cercopithecus aethiops monkey as a reliable model for in vitro study of t cell depletion of bone marrow with campath-1 plus complement. | t-lymphocyte markers of peripheral blood mononuclear cells (pbmcs) and bone marrow mononuclear cells (bmmcs) of c. aethiops monkeys were studied by using anti-human monoclonal antibodies. the results show that c. aethiops t lymphocytes express surface markers which react specifically with anti-human moabs including cd3, cd4, cd8, cd2. however, very few cd3-positive cells were found, in contrast to the abundance in cd8+ cells. there is a high conservation of receptors forming e rosettes with aet- ... | 1989 | 2472335 |
| [expression of ribosomal genes under the action of 5-azacytidine in the african green monkey ramt cell line]. | the use of cytosine analogue--5-azacytidine(5azac), derepression of ribosomal genes has been studied in one of organising chromosomes in the african green monkey ramt cell line in which the nucleolar organizer region (nor) in parental cells was active. the effect of 5azac on the functional state of nor was assessed by the length of the secondary constriction in this chromosome and by the intensity of ag-staining of nor. 5azac was added to the cell culture at concentrations 2-16 m, either immedia ... | 1989 | 2483827 |
| metal release from plates used in jaw fracture treatment. a pilot study. | this study investigated the occurrence of corrosion associated with the use of metallic implants to stabilize jaw fractures. three different types of plates, co-cr and ni-cr alloys and titanium, were connected in vivo to the mandibular bone surface of monkeys (cercopithecus aethiops). the animals were killed after 3 and 6 months. the mucous membrane and bone tissue were analysed for concentrations of co, cr, ni, mo, al, and ti by atomic absorption spectrophometry and a radiochemical neutron acti ... | 1989 | 2509588 |
| inhibition of crimean-congo hemorrhagic fever viral infectivity yields in vitro by ribavirin. | ribavirin was evaluated as a potential therapeutic for crimean-congo hemorrhagic fever (cchf). viral yields for strains of cchf virus from europe, asia, and africa in african green monkey kidney (vero) cells were markedly reduced by this drug. some cchf viral strains appeared more sensitive than others, but in general, ribavirin doses as low as 5 micrograms/ml caused a transient reduction of viral yields. a further reduction in viral yields was induced by a dose of 25 micrograms/ml, and evidence ... | 1989 | 2510529 |
| differential effects of dietary fat on the tissue-specific expression of the apolipoprotein a-i gene: relationship to plasma concentration of high density lipoproteins. | isocaloric substitution of polyunsaturated fat for saturated fat reduces concentrations of total plasma cholesterol and high density lipoproteins (hdl) in nonhuman primates. the biochemical mechanisms through which polyunsaturated fat lowers plasma hdl concentrations are not well understood but must involve changes in hdl production or hdl clearance from plasma, or both. to determine whether dietary polyunsaturated fat (p/s = 2.2) alters apolipoprotein (apo) a-i production, african green monkeys ... | 1989 | 2513367 |
| efficacy of a new hoffmann-la roche compound (ro 15-5458) against schistosoma mansoni (gezira strain, sudan) in vervet monkeys (cercopithecus aethiops). | some compounds of the class 9-acridanone-hydrazones have recently been developed by hoffmann-la roche (basel-switzerland) and were shown to have antischistosomal effects. one of these compounds (ro 15-5458/000) was administered at two dose levels (25 mg and 15 mg/kg body-weight) to s. mansoni (gezira strain-sudan) infected vervet monkeys. the faecal egg-output was terminated, worm-burden killed and tissue egg-counts were greatly reduced as compared with the untreated control monkey. severe necro ... | 1989 | 2515579 |
| enhancement of simian varicella virus infection in african green monkeys by recombinant human tumor necrosis factor alpha. | 1989 | 2536783 | |
| nonhematopoietic tumor cells express functional gm-csf receptors. | human granulocyte-macrophage colony-stimulating factor (gm-csf) stimulates the colony growth of myeloid progenitors in semisolid media, and enhances the function of mature effector cells, including neutrophils, monocytes, and eosinophils. small cell carcinoma lines (sccl) have properties of amine precursor uptake and decarboxylation (apud) cells and express high levels of the enzyme, l-aromatic amino acid decarboxylase. we looked for possible expression of gm-csf receptors on nonhematopoietic ce ... | 1989 | 2537665 |
| liposomal interferon-beta: sustained release treatment of simian varicella virus infection in monkeys. | liposomal formulations (distearoylphosphatidylcholine:dipalmitoylphosphatidylglycerol, 9:1) of recombinant human interferon-beta ser17 (ifn-beta), administered im to african green monkeys at doses of 10(7) units/kg on days 1 and 6 after infection with simian varicella virus, resulted in partial protection of the monkeys from viral infection. aqueous interferon administered under the same dosing regimen was ineffective. when given at 10(6) units/kg per dose twice daily for 10 d, however, it was h ... | 1989 | 2538517 |
| modified sv40 for analysis of mismatch repair in simian and human cells. | we have developed a way of introducing specific mispairs into the genome of simian virus 40 and of determining the fate of the mispaired bases in simian and human cells. mispairs are introduced into viral dna within the intron of the gene coding for the large t antigen. each dna molecule harbors a single mispair in a defined orientation. transfection of mismatch-containing sv40 dna into host cells yields plaques, each corresponding to a productive infection initiated by a single viral dna molecu ... | 1989 | 2538735 |
| release of simian virus 40 virions from epithelial cells is polarized and occurs without cell lysis. | we have investigated the process of release of simian virus 40 (sv40) virions from several monkey kidney cell lines. high levels of virus release were observed prior to any significantly cytopathic effects in all cell lines examined, indicating that sv40 utilizes a mechanism for escape from the host cell which does not involve cell lysis. we demonstrate that sv40 release was polarized in two epithelial cell types (vero c1008 and primary african green monkey kidney cells) grown on permeable suppo ... | 1989 | 2539518 |
| experimental infection of rhesus monkeys with siv isolated from african green monkeys. | to establish an animal model for aids, 6 juvenile rhesus monkeys were infected intravenously with cell-free sivagmtyo-1, 5, or 7. one animal was infected with siv of known pathogenicity isolated from a sooty mangabey (sivsmm). the 2 animals infected with tyo-1, 1 of 2 infected with tyo-7 and the 1 infected with sivsmm seroconverted within 4-8 weeks after infection and infectious virus could be recovered 8-10 or 44 weeks after infection. three of the 4 seroconverted monkeys developed a persistent ... | 1989 | 2540114 |
| a negative regulatory element with properties similar to those of enhancers is contained within an alu sequence. | a negative regulatory element has been found within a member of the african green monkey alu family of interspersed repeated sequences. this "reducer" element decreased transcription in both directions from a cellular simian virus 40-like bidirectional promoter independently of both orientation and position. the reducer was not promoter specific since it also decreased expression from the simian virus 40 early and human beta-globin promoters. in addition, the reducer decreased transcription from ... | 1989 | 2540418 |
| variations in genome fragments coding for rna polymerase in human and simian hepatitis a viruses. | the genome of hepatitis a virus (hav) isolated from spontaneously infected african vervet monkey (cercopithecus aethiops) has been cloned and partially sequenced. comparison of genome fragments (1248 and 162 bp) from the 3d (rna polymerase) region with the corresponding parts of human hav genomes revealed a high degree of heterogeneity: there were altogether 257 nucleotide changes leading to 44 substitutions in predicted amino acid sequence, i.e. 89% amino acid identity. this divergence is consi ... | 1989 | 2541023 |
| vero cells co-infected with chlamydia trachomatis and herpes simplex virus type 2: a scanning and transmission electron microscope study. | vero (african green monkey kidney) cells infected with chlamydia trachomatis (serovar l2) (ct-l2) for 48 hr and superinfected with herpes simplex virus type 2 (hsv-2) were fixed 24 hr later and examined with the transmission electron microscope (tem) and scanning electron microscope (sem). ultrastructural examination of the co-infected cells showed that, although many ct-l2 inclusions were present, most were empty of reticulate bodies or elementary bodies. however, large numbers of viruses were ... | 1989 | 2545004 |
| the role of phosphorylation and limited proteolytic cleavage of talin and vinculin in the disruption of focal adhesion integrity. | chemical agents which activate specific kinases were employed to disrupt the stress fiber and focal adhesion organization of cells spread on a substratum. the phorbol ester 12-o-tetradecanoylphorbol-13-acetate, an activator of protein kinase c, promoted a rapid loss of stress fibers and focal adhesions from african green monkey kidney (bsc-1) cells. this was paralleled by an increase in the level of talin phosphorylation suggesting that this may play a role in the removal of talin from focal adh ... | 1989 | 2545690 |
| actions of complement on junin virus. | fresh sera from normal rhesus monkeys, guinea pigs, and rabbits inactivated 90%-99% of the infectivity of vero cell-passaged, attenuated strains of junin virus (jv) within 60 minutes. selective depletion studies showed that inactivation occurred by the classical complement pathway. complement had little effect on virulent jv strains. adsorption of the fresh sera with jv-infected vero cells showed that inactivation was not mediated by low levels of antibodies in normal sera. the cells used for pr ... | 1989 | 2546249 |
| inhibition of herpes simplex virus infection by pine cone antitumor substances. | several antitumor substances extracted from cones of various pine trees inhibited the plaque formation of herpes simplex virus types 1 and 2 (hsv-1, hsv-2) strains in african green monkey kidney cells and human adenocarcinoma cells. the 50% effective dose of the most active fraction, fr. vi (0.3 micrograms/ml) was 0.001 times its 50% cytotoxic dose (greater than 300 micrograms/ml). the anti-hsv activity of various pine cone extracts increased with their acidity. identification of the polyphenol ... | 1989 | 2546481 |
| effects of dietary cellulose and psyllium husk on monkey colonic microbial metabolism in continuous culture. | the effects of inoculating an in vitro continuous culture system with primate colon contents compared to fecal material, and the effect of feeding these cultures psyllium husk, a fermentable, or cellulose, a less fermentable, dietary fiber were tested. modified 500-ml bellco culture chambers were continuously infused with buffered medium containing vitamin mix, deoxycholate, urea, hemin, casein and mucin. cultures were fed a mixture of minerals, sucrose, starch and either psyllium husk or cellul ... | 1989 | 2547037 |
| effects of dietary cellulose, psyllium husk and cholesterol level on fecal and colonic microbial metabolism in monkeys. | the effect of long-term feeding of dietary fiber and two levels of cholesterol on monkey colonic microbial metabolism was studied. three groups of african green monkeys were fed for 3.5 yr purified diets containing 9.7% cellulose or psyllium husk and 0.8 mg cholesterol per kcal or 9.7% cellulose and 0.1 mg cholesterol per kcal. total viable anaerobe and aerobe counts, microbial beta-glucuronidase activity, volatile fatty acid and ammonia nitrogen concentrations, dry matter and ph were determined ... | 1989 | 2547038 |
| infection of macaque monkeys with simian immunodeficiency virus from african green monkeys: virulence and activation of latent infection. | the virulence of three isolates of simian immunodeficiency virus from african green monkeys (sivagm) was studied in rhesus and pigtailed macaques. none of 15 rhesus monkeys and one of four pigtailed monkeys died from infection during the time they were studied (up to 33 months). sivagm was only isolated from rhesus monkeys for up to 2 months after inoculation. however, when these animals were secondarily infected with simian acquired immunodeficiency syndrome retrovirus type 1 (srv-1), sivagm wa ... | 1989 | 2547960 |
| genetic analysis and infection of sivagm and sivmnd. | recently, the authors determined the partial sequence of simian immunodeficiency virus (siv) from the mandrill (sivmnd) and found sivmnd to be a new member of the hiv/siv group, equidistant from other members, including sivagm. experimentally, the african green monkey and cynomolgus monkey could be infected with sivagm and the cottontop tamarin with sivmnd. however, no clinical sign of an aids-like disease was observed in these monkeys. | 1989 | 2547961 |
| genetic diversity among simian immunodeficiency virus isolates from african green monkeys. | to characterize isolates further within the sivagm subtype, we studied four sivagm isolates by cross-hybridization, molecular cloning, and nucleotide sequencing. our results indicate an unexpected degree of genetic variation among isolates within the sivagm subtype comparable to the variation between sivmac and hiv-2. | 1989 | 2547962 |
| molecular characterization and comparison of simian immunodeficiency virus isolates from macaques, mangabeys, and african green monkeys. | simian immunodeficiency virus (siv)/mne has been inoculated into three species of macaques and into baboons. virus was isolated from all the macaques who subsequently died at 15 to 120 weeks (mean 80 weeks) with various manifestations of immune deficiency. individual animals varied in their viral antibody profile as a function of time after infection. independent siv isolates obtained from african green monkeys and magabeys were compared to siv/mne for their ability to replicate in lymphocytes a ... | 1989 | 2547964 |
| bovine papilloma virus encoded e2 protein activates lymphokine genes through dna elements, distinct from the consensus motif, in the long control region of its own genome. | activation of t cells by antigen, lectin or a combination of phorbol ester (pma) and calcium ionophore (a23187) leads to the induction of a set of lymphokine genes. transfection of a human t cell leukemia cell line, jurkat, or an african green monkey kidney cell line, cv1, with a cdna encoding e2 protein, a trans-activator of bovine papilloma virus type 1, results in activation of interleukin 2 (il-2), interleukin 3 (il-3) and granulocyte/macrophage colony-stimulating factor (gm-csf) genes in a ... | 1989 | 2548843 |
| hominoid triosephosphate isomerase: regulation of expression of the proliferation specific isozyme. | three primary isoforms of the dimeric glycolytic enzyme, triosephosphate isomerase (tpi; ec 5.3.1.1), are detected in proliferating human cells. the electrophoretically separable isoforms result from the three possible combinations of constitutive subunits and subunits expressed only in proliferating cells. only a single primary isoform is observed in quiescent cells. the two subunits, which differ by covalent modification (s), are product of the single structural locus for this enzyme. expressi ... | 1989 | 2550787 |
| members of the rtvl-h family of human endogenous retrovirus-like elements are expressed in placenta. | a cdna clone homologous to the rtvl-h family of human retrovirus-like elements was isolated from a human placenta cdna library. the nucleotide sequence of the 1084-bp cdna revealed an open reading frame (orf) that may encode a 146 amino acid protein with significant homology to retroviral proteases. downstream from the putative protease orf a 3' long terminal repeat (ltr) containing u3 and r regions was found. the cdna sequence ends in a poly(a) tail appropriately positioned downstream from a po ... | 1989 | 2551777 |
| [epizootic hepatitis a among african green monkeys kept in a vivarium]. | the results of observations on the pattern of spread of hepatitis a virus and immune response to it in african green monkeys (cercopithecus aethiops) kept in the animal house are presented. the infection in the monkeys was found to be characterized by all virological, serological, and biochemical parameters inherent in hepatitis a virus. the results indicated that hepatitis a in monkeys may run both asymptomatic and clinically manifest course, and the spread of infection in the animal house sequ ... | 1989 | 2552683 |
| inhibition of herpes simplex virus infection by tannins and related compounds. | several chemically defined plant extracts were investigated for their antiviral action on herpes simplex virus (hsv-1, hsv-2)-infected african green monkey kidney cells and human adenocarcinoma cells, using a plaque formation assay. among them, the monomeric hydrolyzable tannins, oligomeric ellagitannins and condensed tannins, having galloyl groups or hexahydroxydiphenoyl groups, had the most potent anti-hsv activity. their 50% effective doses (0.03-0.1 microgram/ml) were by two-three orders of ... | 1989 | 2552915 |
| immunological and molecular comparison of simian cytomegaloviruses isolated from african green monkey (cercopithecus aethiops) and japanese macaque (macaca fuscata). | simian cytomegaloviruses (cmv) isolated from african green monkeys and japanese macaques were compared serologically and genetically. twenty-three out of 31 sera from japanese, taiwan and rhesus macaques were positive for neutralizing antibodies to simian cmvs isolated from both african green monkeys and a japanese macaque. the neutralizing antibody titers of those sera were slightly higher to the isolates of african green monkeys origin than to that of japanese macaque origin. studies with anti ... | 1989 | 2552962 |
| effect of prolonged infusion of anf in normotensive and hypertensive monkeys. | it is now recognized that bolus and short-term infusions of atrial natriuretic factor (anf) into different species lead to a slight and transient decrease of blood pressure, while prolonged infusions cause a significant blood pressure reduction in hypertensive but not in normotensive rats. the present study was designed to evaluate the effects of prolonged anf infusions on blood pressure and humoral parameters in normotensive and hypertensive african green monkeys (cercopithecus aethiops). human ... | 1989 | 2553071 |
| concentration of poliovirus type i from cow milk using an adsorption-elution method. | cow milk was experimentally contaminated with a vaccine strain of poliovirus type i. a concentration procedure was utilized to assay for the virus in a milk sample using adsorption on aluminium sulphate at ph 4.5-5.5, aluminium sulphate concentration being 0.15 g. 1(-1), followed by elution with 0.1 m na2hpo4 ph 9.5 and subsequent detection in cultured vero cells (kidney cells from the monkey cercopithecus aethiops). the yield of the virus ranged from 14 to 58% of the inoculated amount, the mean ... | 1989 | 2553805 |
| inhibition of sv40 dna replication by rous sarcoma virus ltr enhancer. | simian virus 40 (sv40) late region recombinant constructs containing the rous sarcoma virus (rsv) src gene along with rsv enhancer stimulated expression but completely abolished sv40 dna replication. constructs, in which the heterologous enhancer sequences were omitted, did replicate normally in african green monkey kidney cells and, in the presence of helper virus, gave rise to infectious progeny. inhibition of sv40 dna replication follows a cis-acting mechanism and is most likely due to a conf ... | 1989 | 2554858 |
| efficiency of isolation of human rotavirus in primary african green monkey kidney cells. | out of 212 human rotavirus (hrv) containing fecal specimens, 173 (81.6%) yielded virus on first passage in primary african green monkey kidney cells (agmk), while additional 34 specimens, did not yield virus on first passage. however, following blind passages, 18 of the 34 yielded virus in passage levels 2-8, thus raising the overall isolation rate to 90.1%. the isolation rate of hrv strains obtained in embryonic rhesus monkey kidney cell line (ma-104), was only 41.4%. elisa tests performed on f ... | 1989 | 2555376 |
| molecular cloning of the 3' half of the clostridium perfringens enterotoxin gene and demonstration that this region encodes receptor-binding activity. | clostridium perfringens type a enterotoxin (cpe) causes the symptoms associated with c. perfringens food poisoning. to determine whether the c-terminal half of cpe contains receptor-binding activity, the 3' half of the cpe structural gene was cloned with an escherichia coli expression vector system. e. coli lysates containing the expressed c-terminal cpe fragment (cpefrag) were then assayed for cpe-like serologic, receptor-binding, and cytotoxic activities. cpefrag was shown to contain an epitop ... | 1989 | 2556374 |
| inhibition of growth of chlamydia trachomatis by the calcium antagonist verapamil. | treatment of bgm (african green monkey kidney) cells with the calcium antagonist verapamil resulted in a reduced yield of chlamydial infectious particles. the inhibitory effect was concentration-dependent, the maximal effect being achieved at 200 microm-verapamil, which produced a 99.99% reduction of infectious particle yield. electron microscopy showed that control chlamydia trachomatis-infected bgm cells contained typical large inclusions in which most of the particles were elementary bodies, ... | 1989 | 2559152 |
| primary cells and established cell lines join dna ends with the same efficiency relative to homologous recombination. | the joining of dna ends was compared in the established cv1 cell line and in african green monkey kidney primary cells, from which the cv1 cell line was established. using a linear sv40 genome that carries a terminal repeat of 526 nucleotide pairs, we measured the efficiency of circularization by end joining relative to circularization by homologous recombination between the terminal repeats. the ratios of end joining to homologous recombination were identical in the two types of cells regardles ... | 1989 | 2560220 |
| [topometry of the bronchial tree in ceropithecus aethiops with special regard to presence of precordial lobes]. | the trachea and bronchus were filled with duracryl solution and on the basis of casts got in this way the length and the diameter of individual lobes of the bronchial tree of 14 male and 16 female monkey have been examined. it was ascertained that almost all measurements were greater in the individuals of female sex. in both individuals among segmental basilar bronchus the greatest diameter had medial and anterior bronchus, both in the right and left lung. | 1989 | 2562686 |
| rodent and primate adrenal medullary cells in vitro: phenotypic plasticity in response to coculture with c6 glioma cells or ngf. | in order to maintain a chronic supply of growth factor for medulla cells in vitro, chromaffin cells from rat, african green monkeys and man were co-cultured with c6 glioma cells, which secrete growth factors that sustain sympathetic neurons in vitro. the response of chromaffin cells to coculture was compared to treatment of medullary cells with nerve growth factor (ngf) alone. dispersed chromaffin cell preparations were obtained by a trypsin-collagenase procedure, and subjected to differential p ... | 1989 | 2568944 |