Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| cdna cloning of the polymeric immunoglobulin receptor of the marsupial macropus eugenii (tammar wallaby). | cdna encoding a marsupial polymeric immunoglobulin receptor (pigr) was isolated from macropus eugenii (tammar wallaby) mammary lymph node primarily by reverse transcriptase coupled polymerase chain reaction (rt-pcr) and rapid amplification of cdna ends (race) pcr. this resulted in a 5' truncated clone and, in order to obtain the full-length sequence, genomic walking pcr was utilized. the complete sequence consists of 2696 bp of cdna and encodes a predicted polypeptide of 732 amino acids. the wal ... | 2002 | 11918632 |
| molecular cloning of four lambda light chain cdnas from the australian brushtail possum trichosurus vulpecula. | a brushtail possum mesenteric lymph node cdna library was screened with a grey short-tail opossum clambda probe and four immunoglobulin lambda cdnas were isolated. two of the isolated clones (l5 and l10) contained identical framework 4 regions and constant regions (but different variable regions), suggesting that the possum lambda locus is organized as multiple j-c pairs--a feature seen in the opossum and placental mammals. the cloning of the lambda light chain cdnas signifies the completion of ... | 2002 | 11918633 |
| ovarian expression of messenger rna encoding the receptors for luteinizing hormone and follicle-stimulating hormone in a marsupial, the brushtail possum (trichosurus vulpecula). | both lh and fsh play a central role in controlling ovarian function in mammals. however, little is known about the type of ovarian cells that are responsive to lh and fsh in marsupials. we determined, using in situ hybridization, the localization of mrna encoding the receptors (r) for lh and fsh in ovaries of brushtail possums. the mrna encoding fsh-r was observed in granulosa cells of healthy follicles containing at least two complete layers of cells. the mrna encoding lh-r was first observed i ... | 2002 | 11967192 |
| bait-delivered cabergoline for the reproductive control of the red fox (vulpes vulpes): estimating mammalian non-target risk in south-eastern australia. | cabergoline (cab) is a potent dopamine agonist and an inhibitor of prolactin (prl). in red foxes (vulpes vulpes), a single oral dose of 100 microg kg(-1) cab can cause abortions and postnatal cub mortality from at least day 21 of the 52-day pregnancy. the abortifacient activity of cab is owing to the suppression of prl, which is essential for luteotrophic support in some eutherian species. postnatal cub mortality probably results from a reduction in the development of the mammary ductile system, ... | 2001 | 11999299 |
| the corpus luteum and interstitial tissue in a marsupial, the brushtail possum (trichosurus vulpecula). | the australian brushtail possum (trichosurus vulpecula) is a nocturnal, arboreal marsupial. it has become a pest of significant ecological and economic importance in new zealand, and thus a renewed interest in understanding the reproductive biology of this species has been generated. the corpus luteum (cl) in possums is a largely autonomous gland in that it does not rely on pituitary hormones to function and is not responsive to luteolytic agents for its demise. its importance in regulating the ... | 2002 | 12044921 |
| microsomal metabolism and enzyme kinetics of the terpene p-cymene in the common brushtail possum (trichosurus vulpecula), koala (phascolarctos cinereus) and rat. | 1. p-cymene is an aromatic monoterpene found in the leaves of eucalyptus spp. and is ingested in the diet of two marsupial folivores, the brushtail possum (trichosurus vulpecula) and koala (phascolarctos cinereus). the metabolism of p-cymene by liver microsomes from the possum, koala and rat (an animal whose diet is not terpene-rich) was examined. 2. the major p-cymene metabolite in each species was cuminyl alcohol, with smaller amounts of other side-chain alcohols being formed. no phenolic meta ... | 2002 | 12065061 |
| plasma growth hormone and growth hormone-binding protein during development in the marsupial brushtail possum (trichosurus vulpecula). | plasma concentrations of growth hormone (gh) were measured in the brushtail possum (trichosurus vulpecula) pouch young from 25 through to 198 days post-partum (n=71). gh concentrations were highest early in pouch life (around 100 ng/ml), and thereafter declined in an exponential fashion to reach adult concentrations (10.8+/-1.8 ng/ml; n=21) by approximately 121-145 days post-partum, one to two months before the young is weaned. growth hormone-binding protein (ghbp), which has been shown to modif ... | 2002 | 12065240 |
| ontogeny of immunoglobulin expression in the brushtail possum (trichosurus vulpecula). | marsupials, unlike eutherians, are born immunologically immature, without circulating lymphocytes or organised lymphoid tissue. their immune response develops while they are in the pouch not in the uterus. in this study, the onset time of immunoglobulin expression in trichosurus vulpecula pouch young was estimated by reverse transcription polymerase chain reaction. as in eutherian species, igm heavy chain transcripts were detected first, at day 10 post partum. the first switched transcript, dete ... | 2002 | 12074924 |
| characterisation of antisera to recombinant iga of the common brushtail possum (trichosurus vulpecula). | one of the limiting factors in understanding immune responses in marsupials is the scarcity of marsupial specific immunological reagents. this paper describes the characterisation of an antiserum raised against a recombinant protein of the constant region of the heavy chain of iga (c(alpha)) of the common brushtail possum (trichosurus vulpecula). the availability of a marsupial specific anti-iga provides a useful tool for the characterisation of mucosal immune responses in possums. anti-c(alpha) ... | 2002 | 12088648 |
| expression of mrna encoding growth differentiation factor 9 and bone morphogenetic protein 15 during follicular formation and growth in a marsupial, the brushtail possum (trichosurus vulpecula). | the oocyte derived growth differentiation factor (gdf) 9 and bone morphogenetic protein 15 (bmp15; also known as gdf9b) are essential for normal follicular growth. however, little is known about expression of these factors during ovarian development. therefore, we determined the ontogeny of expression of gdf9 and bmp15 mrna in the developing ovary of the brushtail possum. ovaries were collected from pouch young (n=3-5 per group) around times of key developmental events namely: (1) morphological ... | 2002 | 12088873 |
| putative osmolytes in the kidney of the australian brush-tailed possum, trichosurus vulpecula. | the australian brush-tailed possum, trichosurus vulpecula, is capable of producing a moderately concentrated urine, at least up to 1300 mosm l(-1). kidneys of adult animals fed in captivity on a normal diet with ready access to water were analysed. the inner medullary regions were found to have moderately high concentrations of sodium (outer medulla, 367+/-37; inner medulla 975+/-93 mmol kg(-1) dry wt.), chloride (outer medulla 240+/-21; inner medulla 701+/-23 mmol kg(-1) dry wt.) and urea (oute ... | 2002 | 12091109 |
| the effects of hypoosmotic infusion on the composition of renal tissue of the australian brush-tailed possum trichosurus vulpecula. | although the occurrence of organic osmolytes in the inner medulla of the marsupial kidney has been recently reported [comp. biochem. physiol. (2002) 132b 635-644], changes in these substances, in response to water loading in vivo, has not been studied. adult trichosurus vulpecula, the australian brush-tailed possum, were subjected to water deprivation for 48 h. following anaesthesia and unilateral nephrectomy, the animals were perfused with hypo-osmotic saline (80 mmol l(-1); 1.5 ml min(-1)) for ... | 2002 | 12091110 |
| in vitro and in vivo maturation of oocytes from gonadotrophin-treated brushtail possums. | the time course of nuclear maturation of oocytes was examined in brushtail possums, trichosurus vulpecula. oocytes were recovered from ovarian follicles > 2 mm in diameter after pregnant mares' serum gonadotrophin/porcine luteinizing hormone (pmsg/lh) treatment (in vivo matured) or 72 hr after pmsg treatment (in vitro matured). oocytes recovered from small (< 2 mm) and large (> 2 mm) follicles were also assessed for their ability to mature in vitro. staining with the dna-specific dye hoechst 333 ... | 2002 | 12112584 |
| mucosal immunity in the brushtail possum (trichosurus vulpecula): detection of antibody in serum and at female reproductive sites after intranasal immunization. | vaccination strategies for the brushtail possum, which rely upon stimulation of mucosal immunity, are being developed for biocontrol purposes. as little is known about how to stimulate possum immune responses via a mucosal site, groups of possums were immunized intranasally with keyhole limpet haemocyanin (klh) alone or in combination with known or novel mucosal adjuvants. antigen-specific antibody titres in female reproductive secretions were measured by elisa and compared with antibody titres ... | 2002 | 12121225 |
| electrogenic ion transport in the intestine of the australian common brushtail possum, trichosurus vulpecula: indications of novel transport patterns in a marsupial. | in this study, electrogenic ion transport in the intestine of the australian common brushtail possum, trichosurus vulpecula was investigated. in the ileum, a na(+)-dependent, phloridzin- and amiloride-insensitive short-circuit current ( isc) was present. mucosal glucose stimulated a further phloridzin-sensitive, dose-dependent increase in isc. a na(+)-dependent, ouabain-sensitive isc was also present in the caecum and colon. in the proximal and distal colon, amiloride (100 micro mol l(-1), mucos ... | 2002 | 12192511 |
| aldosterone does not regulate amiloride-sensitive na(+) transport in the colon of the australian common brushtail possum, trichosurus vulpecula. | plasma aldosterone concentrations and the magnitude of amiloride-sensitive na(+) transport in the proximal and distal colon of newly caught wild possums, and of possums maintained on either low or high na(+) diets, were determined to evaluate their role in the regulation of salt content of faeces in common brushtail possums. wild animals had a plasma aldosterone concentration of 439+/-115 pmol l(-1) and high levels of amiloride-sensitive na(+) transport in both the proximal and distal colon. ani ... | 2002 | 12192514 |
| quantification of mast cells and microflora in the vaginal cul-de-sac of the brushtail possum (trichosurus vulpecula). | mast cells are typically associated with biological responses to intracellular parasitic or bacterial invasion in gut and pulmonary tissues. large numbers of these cells are present in the vaginal cul-de-sac, a component of the reproductive tract in female brushtail possums (trichosurus vulpecula). the presence of mast cells in this area may reflect microbial presence or indicate a role for mast cells in reproductive function in this marsupial. in this study, the number and spatial location of m ... | 2002 | 12201813 |
| the efficacy of bacille calmette-guérin vaccine in wild brushtail possums (trichosurus vulpecula). | a population of wild brushtail possums (trichosurus vulpecula) in which bovine tuberculosis was endemic was vaccinated with live bacille calmette-guérin (bcg) to determine the efficacy of vaccination. the population on the 56 hectare site was monitored bimonthly over 2 years using a capture-release regime. during the study tuberculosis was diagnosed by clinical and post mortem examination. possums were vaccinated with bcg by both intranasal aerosol and conjunctival instillation. possums were rev ... | 2002 | 12204633 |
| protein and peptide degradation in the intestine of the common brushtail possum (trichosurus vulpecula). | a protein (bovine serum albumin: bsa) and a peptide (luteinizing hormone releasing hormone: lhrh) were used to evaluate proteolytic activity in the intestine of common brushtail possums (marsupiala, trichosurus vulpecula). luminal and mucosal extracts were isolated from the duodenum, jejunum, ileum, caecum, proximal colon and distal colon, their protein content assessed and specific activities in metabolising lhrh and bsa determined in vitro. the degradation of lhrh by luminal extracts was compa ... | 2002 | 12355223 |
| the effect of excesses and deficiencies in amino acids on the feeding behaviour of the common brushtail possum (trichosurus vulpecula). | in this study of the amino acid nutrition of a marsupial we tested three hypotheses: (a) that brushtail possums eat less when diets contain excesses or deficiencies in essential amino acids, (b) that brushtail possums choose diets that do not contain amino acid excesses, and (c) that amino acid consumption is mediated partly by the 5ht3 receptor. possums ate less when 0.2-1.0% methionine (wet matter) was added to the diet, but similar concentrations of lysine and threonine had little effect. how ... | 2002 | 12355229 |
| characterisation of echidna igm provides insights into the time of divergence of extant mammals. | the immunobiology of monotremes is poorly understood. in this paper, we describe the characterisation of the heavy chain of igm from tachyglossus aculeatus, the short-beaked echidna. the echidna heavy chain constant region of igm (cmu)was isolated from a spleen cdna library using a trichosurus vulpecula probe. it has approximately 46.5% amino acid identity to marsupial and eutherian cmus, and approximately 30% amino acid identity with cmu from birds and reptiles. phylogenetic analysis of mammali ... | 2002 | 12377222 |
| enzymatic degradation of luteinizing hormone releasing hormone (lhrh) by mucosal homogenates from the intestine of the common brushtail possum (trichosurus vulpecula). | the peptidolytic activity of fresh and frozen mucosal homogenates from five regions (duodenum, jejunum, ileum, caecum and colon) of possum intestine from trichosurus vulpecula towards human luteinizing hormone releasing hormone (lhrh) was investigated. the rank of order of specific peptidolytic activity of the mucosal homogenates was jejunum > ileum > caecum> duodenum = colon, with a 3 to 4 fold difference between the least and the most active segment in both frozen and fresh samples. the format ... | 2002 | 12384185 |
| application of solid-phase microextraction to the quantitative analysis of 1,8-cineole in blood and expired air in a eucalyptus herbivore, the brushtail possum (trichosurus vulpecula). | we have developed two solid-phase microextraction (spme) methods, coupled with gas chromatography, for quantitatively analysing the major eucalyptus leaf terpene, 1,8-cineole, in both expired air and blood from the common brushtail possum (trichosurus vulpecula). in-line spme sampling (5 min at 20 degrees c room temperature) of excurrent air from an expiratory chamber containing a possum dosed orally with 1,8-cineole (50 mg/kg) allowed real-time semi-quantitative measurements reflecting 1,8-cine ... | 2002 | 12401367 |
| production of a biologically active recombinant marsupial growth factor using the methylotrophic yeast pichia pastoris. | the cytokine stem cell factor (scf) and its interaction with its receptor c-kit plays an important role in the development of germ cells in eutherians. to investigate the putative roles of the scf/c-kit system in marsupials, recombinant australian brushtail possum (trichosurus vulpecula) scf was purified after secretion by the methylotrophic yeast pichia pastoris. the purification procedure utilized ni2+ affinity chromatography with a poly-histidine tag engineered onto the c-terminus of the reco ... | 2002 | 12467357 |
| expression of anti-müllerian hormone mrna during gonadal and follicular development in the brushtail possum (trichosurus vulpecula). | the ontogeny of anti-müllerian hormone (amh) gene expression in the brushtail possum during formation of the ovary and growth of follicles was examined using in situ hybridization. for comparative purposes, the expression pattern of amh was also examined in the developing testis. in the female, amh mrna was observed in the ovary of 50% (3/6) of pouch young collected around the time of sexual differentiation of the gonad (days 1-5): the signal was predominately localized to the inner-cortical and ... | 2002 | 12467360 |
| expression in escherichia coli and immunological characterization of three zona pellucida proteins (zp1, zp2, and zp3) from a marsupial, the brushtail possum (trichosurus vulpecula). | the brushtail possum (trichosurus vulpecula) zona pellucida (zp) is composed of three major glycoproteins, designated zp1, zp2, and zp3 based on their size and homology with eutherian zp proteins. these proteins are candidate antigens for the development of an immunocontraceptive vaccine to control the fertility of the brushtail possum in new zealand, where it is an introduced pest. in order to further their immunological and functional characterization, recombinant possum zp proteins were produ ... | 2003 | 12506345 |
| use of released pigs as sentinels for mycobacterium bovis. | identifying the presence of bovine tuberculosis (tb; mycobacterium bovis) in wildlife is crucial in guiding management aimed at eradicating the disease from new zealand. unfortunately, surveys of the principal wildlife host, the introduced brushtail possum (trichosurus vulpecula), require large samples (> 95% of the population) before they can provide reasonable confidence that the disease is absent. in this study, we tested the feasibility of using a more wide-ranging species, feral pig (sus sc ... | 2002 | 12528431 |
| expression of the fcrn receptor (alpha and beta) gene homologues in the intestine of suckling brushtail possum (trichosurus vulpecula) pouch young. | the neonatal igg transporter fcrn consists of two chains, fcrn alpha and beta (also known as beta(2) microglobulin), and is involved in transferring igg molecules across both mammary and intestinal epithelial cells. developmental changes in fcrn igg alpha and beta chain mrna levels were investigated in the gut of brushtail possum (trichosurus vulpecula) pouch young (py) using northern hybridisation. fcrn alpha transcripts were detected in the py proximal intestine at all times examined, between ... | 2003 | 12531282 |
| the gh/igf-i axis in the brushtail possum (trichosurus vulpecula) pouch young. | plasma and pituitary gh concentrations and liver gh receptor (ghr), igf-i and igf-binding protein-3 (igfbp-3) mrna expression were determined in brushtail possum (trichosurus vulpecula) pouch young aged 12-150 days post-partum and in adults. mean plasma gh concentrations were highest, measuring around 150 ng/ml, from 12 to 100 days post-partum, and thereafter declined so that by 150 days post-partum levels were not significantly different from those in adults (10.8+/-1.8 ng/ml (s.e.m.)). in cont ... | 2003 | 12553870 |
| differential susceptibility to eucalyptus secondary compounds explains feeding by the common ringtail (pseudocheirus peregrinus) and common brushtail possum (trichosurus vulpecula). | the effect of two plant secondary metabolites, tannins and formylated phloroglucinol compounds (fpcs), on the intake of eucalyptus foliage by common ringtail (pseudocheirus peregrinus) and common brushtail possums (trichosurus vulpecula) was studied. we manipulated the amount of tannin that was free to bind with protein by coating foliage with polyethylene glycol 4000 (peg) and relied on natural intraspecific variation in fpc concentrations. in contrast to ringtail possums, brushtail possums sho ... | 2003 | 12592445 |
| the lymphoid and immunohaematopoietic tissues of the embryonic brushtail possum ( trichosurus vulpecula). | the lymphoid and immunohaematopoietic tissues of the embryonic and full-term brushtail possums was investigated histologically and immunohistochemically using antibodies to the t- and b-cell markers, cd3, cd5, cd79a and cd79b. no clearly defined thymus, bone marrow, spleen, lymph nodes, gut-associated lymphoid tissues or bronchus-associated lymphoid tissues were observed histologically. the liver was haematopoietic and contained erythrocytic and granulocytic precursors. no mature lymphocytes wer ... | 2003 | 12592570 |
| inhibition of the microsomal metabolism of 1,8-cineole in the common brushtail possum (trichosurus vulpecula) by terpenes and other chemicals. | 1. brushtail possums (trichosurus vulpecula) ingest large amounts of terpenes in their diet of eucalyptus leaf. previously, we showed that dietary terpenes induce the cytochrome p450 enzymes (cyps) responsible for their metabolism. the present study examined the effects of various cyp inhibitors on the metabolism of 1,8-cineole, the major dietary terpene, by liver microsomes from the possum and rat. 2. ketoconazole inhibited the major reactions of terpene-induced microsomes in both species: 9-hy ... | 2002 | 12593759 |
| development of a porcine follicle-stimulating hormone and porcine luteinizing hormone induced ovulation protocol in the seasonally anoestrus brushtail possum (trichosurus vulpecula). | monovulatory brushtail possums (trichosurus vulpecula) were stimulated with exogenous hormones during seasonal anoestrus to overcome ovarian insensitivity and induce ovulation. seasonal ovarian insensitivity to pregnant mare serum gonadotrophin (pmsg) was overcome by a new porcine follicle-stimulating hormone/porcine luteinizing hormone (pfsh/plh) protocol. this protocol was refined because the original treatment produced oocytes with abnormal morphology. possums (n = 12 per group) received eigh ... | 2002 | 12617789 |
| species differences in the ovarian distribution of 3beta-hydroxysteroid dehydrogenase/delta5-->4 isomerase (3beta-hsd) in two marsupials: the brushtail possum trichosurus vulpecula and the grey, short-tailed opossum monodelphis domestica. | the ovarian distribution of the steroidogenic enzyme 3beta-hydroxysteroid dehydrogenase/delta(5-->4) isomerase (3beta-hsd) was investigated by immunocytochemistry in two marsupial species throughout the reproductive cycle, using a rabbit polyclonal antibody raised against human placental 3beta-hsd. in the polyoestrous and polyovular south american opossum monodelphis domestica, immunostaining was positive for 3beta-hsd in the adrenal cortex, the ovarian interstitial tissue, the corpus luteum and ... | 2003 | 12622697 |
| cloning and expression of a novel cdna encoding shell coat protein, cp4, from the brushtail possum (trichosurus vulpecula). | the marsupial conceptus is enclosed by several egg coats of evolutionary significance and unknown composition, of which the shell coat in mammals occurs only in marsupials and monotremes. intact coats are vital to marsupial embryonic development. towards a better understanding of the marsupial coat proteins, a cdna sequence (cp4) encoding a shell coat protein was cloned from the brushtail possum. a cdna library of a zygote stage uterus was screened using a deduced oligonucleotide sequence based ... | 2003 | 12704724 |
| effect of oestradiol treatment on mast cell populations and microflora in the vaginal cul-de-sac of seasonally anoestrous brushtail possums (trichosurus vulpecula). | mast cell populations in the vaginal cul-de-sac of female brushtail possums do not appear to be related to microbial invasion but changes in their density occur at oestrus, indicating a hormonal influence. the present study examined the effect of treatment with oestradiol on microflora and on mast cell numbers and their spatial location in cul-de-sac tissue of seasonally anoestrous brushtail possums. tissue was collected from seasonally anoestrous brushtail possums (n = 6 per group) that were ei ... | 2003 | 12713436 |
| determination of steroidogenic potential of ovarian cells of the brushtail possum (trichosurus vulpecula). | the ovary of the brushtail possum (trichosurus vulpecula) secretes steroids; however, little is known about the identity of the steroidogenic cells in the ovary. the aim of the present study was to determine the identity of the ovarian cell types expressing mrnas encoding proteins important for steroidogenesis and determine at what stage of follicular development they are expressed. the genes examined were those for steroidogenic factor-1 (sf-1), steroidogenic acute regulatory protein (star), cy ... | 2003 | 12773431 |
| social-network analysis of mycobacterium bovis transmission among captive brushtail possums (trichosurus vulpecula). | wild brushtail possums (trichosurus vulpecula) are the main source of mycobacterium bovis infection for new zealand livestock. the disease is spread principally by infectious aerosol; therefore, social interactions determine disease transmission. in captive possums, den-sharing behaviour provided the greatest risk of tuberculosis transmission between animals. den sharing between individual possums was used as the basic measurement for quantifying close proximity between animals over extended per ... | 2003 | 12809760 |
| characterization of the intrinsic and extrinsic innervation of the gall bladder epithelium in the australian brush-tailed possum (trichosurus vulpecula). | intrinsic neurones of the gall bladder modulate its function. nitric oxide synthase (nos) and vasoactive intestinal polypeptide (vip) are present in gall bladder neurones and nitric oxide and vip modulate its epithelial functions. as an extensive extrinsic innervation of the gall bladder is also present, the source of the epithelial innervation is unclear. in this study the source of the gall bladder epithelial innervation is defined. immunoreactivity for vip, nos, substance p (sp), calcitonin g ... | 2003 | 12846726 |
| experimental infection of brushtail possums (trichosurus vulpecula) with mycobacterium bovis by conjunctival instillation. | in new zealand, the brushtail possum (trichosurus vulpecula) is the major wildlife reservoir of mycobacterium bovis. procedures for experimentally infecting possums are required to study the pathogenesis of the disease and to challenge possums in vaccine efficacy studies. conjunctival instillation of a suspension of m. bovis was effective in producing bovine tuberculosis in captive possums. the experimental disease progressed slowly with the development of palpable lesions in superficial lymph n ... | 2003 | 12902183 |
| contingency discriminability, matching, and bias in the concurrent-schedule responding of possums (trichosurus vulpecula). | six possums (trichosuruus vulpecula) responded under dependent concurrent variable-interval variable-interval schedules of reinforcement. over 15 conditions, barley-carob was one reinforcer with the other reinforcer consisting of coco pops, coconut, or a barley-carob mixture with 0%, 2%, 4%, or 6% salt added to the barley. the schedules were both variable-interval 40 s. as has been found with other species, behavior on the concurrent schedules was biased by the type of feed, with the 6% salt and ... | 2003 | 12908759 |
| effects of two plant secondary metabolites, cineole and gallic acid, on nightly feeding patterns of the common brushtail possum. | we investigated effects of two plant secondary metabolites (psms), cineole and gallic acid, on the nightly feeding behavior of the common brushtail possum (trichosurus vulpecula), a generalist folivore. we tested whether possums altered their feeding behavior in response to increasing levels of cineole, a dietary terpene. possums were fed artificial diets containing three levels of cineole: zero (basal diet), medium (6.8% of total dry matter, dm), and high (15.3% dm). in another experiment, we i ... | 2003 | 12918927 |
| an experimental study of the central olfactory connexions in a marsupial (trichosurus vulpecula). | 1953 | 13081884 | |
| observations on carbohydrate metabolism and alloxan diabetes in a marsupial (trichosurus vulpecula). | 1954 | 13159768 | |
| the carotid sinus complex, "parathyroid" iii and thymo-parathyroid bodies, with special reference to the australian opossum, trichosurus vulpecula. | 1955 | 13258505 | |
| glucuronuria in a herbivorous marsupial trichosurus vulpecula. | 1957 | 13425989 | |
| growth of the marsupial trichosurus vulpecula and a comparison with some higher mammals. | 1957 | 13490833 | |
| variations in the erythrocyte potassium and sodium in the possum, trichosurus vulpecula. | 1958 | 13517187 | |
| properties of the erythrocytes of two marsupials: the brush-tail possum, trichosurus vulpecula (kerr) and the quokka, setonix brachyurus (quoy & gaimard). | 1961 | 13687012 | |
| a degeneration study of the somaesthetic afferent systems in the marsupial phalanger, trichosurus vulpecula. | 1961 | 13694086 | |
| somaesthetic pathways in the marsupial phalanger, trichosurus vulpecula. | 1961 | 13721896 | |
| composition of milk of the marsupial trichosurus vulpecula. | 1959 | 13829661 | |
| interstitial cells in the testis of the australian phalanger (trichosurus vulpecula). | 1963 | 13950092 | |
| the initiation and maintenance of lactation in the marsupial, trichosurus vulpecula. | 1962 | 13976894 | |
| reproduction in the marsupial trichosurus vulpecula. | 1962 | 14037337 | |
| steroid components in the adrenal venous blood of trichosurus vulpecula (kerr). | 1964 | 14199276 | |
| the effects of eucalyptus terpenes on hepatic cytochrome p450 cyp4a, peroxisomal acyl coa oxidase (aox) and peroxisome proliferator activated receptor alpha (pparalpha) in the common brush tail possum (trichosurus vulpecula). | eucalyptus leaves contain a high proportion of essential oils comprising of a complex mixture of monoterpenes such as 1,8-cineole, alpha-pinene, d-limonene and p-cymene. in this study, hepatic levels of microsomal lauric acid hydroxylase and peroxisomal cyanide-intensive palmitoyl coenzyme a oxidative activities were examined in livers of possums given an artificial diet consisting of the above monoterpenes for 10 days. these values were compared with those of possums fed a control diet containi ... | 2003 | 14559298 |
| different susceptibility of two animal species infected with isogenic mutants of mycobacterium bovis identifies phot as having roles in tuberculosis virulence and phosphate transport. | the mycobacterium tuberculosis complex includes mycobacterium bovis, which causes tuberculosis in most mammals, including humans. in previous work, it was shown that m. bovis atcc 35721 has a mutation in its principal sigma factor gene, siga, causing a single amino acid change affecting binding of siga with the accessory transcription factor whib3. atcc 35721 is avirulent when inoculated subcutaneously into guinea pigs but can be restored to virulence by integration of wild-type siga to produce ... | 2003 | 14600232 |
| body size, age and paternity in common brushtail possums (trichosurus vulpecula). | sexual selection should produce sexual size dimorphism in species where larger members of one sex obtain disproportionately more matings. recent theory suggests that the degree of sexual size dimorphism depends on physical and temporal constraints involving the operational sex ratio, the potential reproductive rate and the trade-off between current reproductive effort and residual reproductive value. as part of a large-scale experiment on dispersal, we investigated the mating system of common br ... | 2004 | 14653799 |
| characterisation of and immunity to the aerobic bacteria found in the pouch of the brushtail possum trichosurus vulpecula. | the bacterial composition of the brustail possum (trichosurus vulpecula) pouch was characterized throughout the reproductive cycle using brushtails from an australian captive breeding colony (45 swabs) and a wild population in new zealand (26 swabs). gram-positive coccal species predominate throughout the reproductive cycle. enteric gram-negative rods, particularly escherichia coli, were most prevalent when a pouch young was present and was most likely the result of faecal contamination from the ... | 2004 | 14656540 |
| experimental prostatectomy in a marsupial (trichosurus vulpecula). | 1950 | 14779569 | |
| transmission of chagas' disease to the australian marsupial trichosurus vulpecula. | 1951 | 14835791 | |
| a flow cytometric assay for global estimation of tyrosine phosphorylation associated with capacitation of spermatozoa from two marsupial species, the tammar wallaby (macropus eugenii) and the brushtail possum (trichosurus vulpecula). | the phosphorylation of tyrosine residues in cellular proteins is a major signal transduction event during sperm capacitation. in this study protein phosphorylation was monitored using a fluorescein isothiocyanate (fitc)-labeled antiphosphotyrosine monoclonal antibody and a flow cytometric procedure optimized for sperm. using this technique, the correlation between tyrosine phosphorylation and sperm capacitation was examined in two marsupial species, the brushtail possum and the tammar wallaby an ... | 2004 | 15056774 |
| effect of steroid hormones on tissue remodelling and progesterone receptors in the uterus of seasonally anoestrous brushtail possums (trichosurus vulpecula). | this study describes progesterone receptor (pr) location within uterine cells and associated morphological changes to the uterine luminal and glandular epithelium in seasonally anoestrous brushtail possums treated with oestradiol and/or progesterone. twenty-four adult female possums (n = 6/group) were treated with oestradiol, progesterone, oestradiol followed by progesterone or with the oil vehicle alone for 6-day periods. uterine tissue was recovered, weighed and processed for light and transmi ... | 2004 | 15056791 |
| ionic calcium levels in oviduct explant-conditioned media from an australian marsupial, the brushtail possum (trichosurus vulpecula) and its relevance to in vitro fertilization. | gametes from the brushtail possum (trichosurus vulpecula), an australian marsupial, require exposure to oviductal cells and/or their secretions before sperm binding and penetration of the zona pellucida can occur. sperm-egg fusion, the next critical step in fertilization has not previously been reported in vitro. here we describe the refinement of an oviduct epithelial cell (oec) explant culture system using two different media to obtain in vitro sperm-egg fusion in the brushtail possum for the ... | 2003 | 15085727 |
| normal levels of immunocompetence in possums (trichosurus vulpecula) exposed to different laboratory housing conditions post capture. | specific and non-specific immunological tests were used to monitor aspects of the immune response in captive possums. the tests included total and differential white blood cell counts, lymphocyte transformation assay, and enzyme linked immunosorbant assay. the level of free cortisol present in possum plasma samples was evaluated as an endocrine marker for stress. four different housing conditions were used to test whether stress could be managed or avoided in captive animals. animals were caged ... | 2004 | 15186255 |
| high microsatellite diversity and differential structuring among populations of the introduced common brushtail possum, trichosurus vulpecula, in new zealand. | an understanding of genetic variation and structure of pest populations has the potential to improve the efficiency of measures to control them. genetic analysis was undertaken at five microsatellite loci in four native australian and 14 introduced new zealand populations of the common brushtail possum trichosurus vulpecula in order to document these parameters. genetic variation in new zealand populations, and phylogenetic relationships among australian and new zealand populations, were largely ... | 2004 | 15219155 |
| constraint of feeding by chronic ingestion of 1,8-cineole in the brushtail possum (trichosurus vulpecula). | eucalyptus leaf-eating marsupials such as the brushtail possum (trichosurus vulpecula) ingest large amounts of terpenes, especially 1,8-cineole (cineole)--the major component of many eucalyptus oils. brushtail possums were acclimated to a non-eucalyptus diet with increasing concentrations of cineole (0.5-4.0% wet weight) added over 18 d. we measured food and cineole consumption and urinary metabolites of cineole. food intake decreased with cineole content, indicating that it was constrained by t ... | 2004 | 15260222 |
| cycle of the seminiferous epithelium in a marsupial species, the brushtail possum (trichosurus vulpecula), and estimation of its duration. | we investigated the cycle of the seminiferous epithelium in a marsupial, namely the brushtail possum (trichosurus vulpecula), using semithin sections of seminiferous tubules embedded in spurr's resin. using 14 steps of spermatid development as markers, we were able to class tubular cross-sections into 10 well-defined stages of the seminiferous epithelial cycle. the duration of one cycle was 13.5 days, as determined by injections of [(3)h]-thymidine and autoradiographic examination of the most ad ... | 2004 | 15304203 |
| assessment of anti-bovine il4 and ifn gamma antibodies to label il4 and ifn gamma in lymphocytes of the koala and brushtail possum. | we assess anti-bovine il4 and ifn gamma (ifng) antibodies for their ability to label il4 and ifng in koala (phascolarctos cinereus), common brushtail possum (trichosurus vulpecula) and mountain brushtail possum (trichosurus caninus) lymphocytes using flow cytometry and immunohistochemistry to determine their applicability to studies of host response to intracellular pathogens. anti-ifng labelled a product of pma-ionomycin stimulated sheep, koala and possum lymphocytes. high intensity labelling w ... | 2004 | 15350745 |
| a method for the collection of the secretions of the individual accessory sex glands in a marsupial (trichosurus vulpecula). | 1950 | 15423051 | |
| seasonal changes in mesotocin and localization of its receptor in the prostate of the brushtail possum (trichosurus vulpecula). | the prostate gland in the brushtail possum grows and regresses seasonally. it has similarities to the human prostate and may therefore provide a unique model for investigating prostatic hyperplasia. oxytocin has been implicated in the regulation of prostate growth in eutherian mammals, and the initial aim of this study was to identify and localize the marsupial equivalent, mesotocin, and its receptor in the prostate of the brushtail possum. seasonal changes in prostatic mesotocin concentrations ... | 2005 | 15496513 |
| demography in relation to population density in two herbivorous marsupials: testing for source-sink dynamics versus independent regulation of population size. | we compared demography of populations along gradients of population density in two medium-sized herbivorous marsupials, the common brushtail possum trichosurus vulpecula and the rufous bettong aepyprymnus rufescens, to test for net dispersal from high density populations (acting as sources) to low density populations (sinks). in both species, population density was positively related to soil fertility, and variation in soil fertility produced large differences in population density of contiguous ... | 2005 | 15583939 |
| effects of nutrient variability on the genetic-based resistance of eucalyptus globulus to a mammalian herbivore and on plant defensive chemistry. | plant resistance to herbivores can be influenced not only by the independent effects of plant genotype and environmental variation, but by interactions between the two. the main aim of this study was to assess the effects of environmental variability (nutrient treatment) on the known genetic-based expression of resistance and defensive chemistry of eucalyptus globulus to browsing by the generalist mammalian herbivore trichosurus vulpecula. in a captive feeding trial, we measured intake of seedli ... | 2005 | 15583943 |
| vaccination of brushtail possums, trichosurus vulpecula, with bacille calmette-guerin induces t lymphocytes that reduce mycobacterium bovis replication in alveolar macrophages via a contact-dependent/nitric oxide-independent mechanism. | the permissiveness of alveolar macrophages from brushtail possums for the replication of mycobacterium bovis was examined. mycobacterium bovis replication was indirectly measured by assessing bacterial metabolism via the incorporation of [3-h]-uracil by bacilli released from lysed macrophages previously infected with mycobacteria. alveolar macrophages allowed substantial replication of virulent m. bovis, in contrast to bacille calmette-guerin (bcg) pasteur, which replicated poorly. the addition ... | 2005 | 15661042 |
| mapping of b cell epitopes on the zona pellucida 2 protein of a marsupial, the brushtail possum (trichosurus vulpecula). | immunocontraceptive vaccines against zona pellucida (zp) proteins are being developed for brushtail possum (trichosurus vulpecula) management in new zealand. mapping of b cell epitopes on the zp2 protein of possums was undertaken in this study to define the antigenic regions that may be crucial to sperm-egg binding. the amino acid sequence of the full-length possum zp2 protein (712 amino acids) was used to synthesize a complete set of 71 (15-mer) biotinylated peptides with an offset of five amin ... | 2005 | 15685627 |
| plant-based immunocontraceptive control of wildlife--"potentials, limitations, and possums". | possums (trichosurus vulpecula), originally introduced from australia, are spread over 90% of new zealand and cause major economic and environmental damage. immunocontraception has been suggested as a humane means to control them. marsupial-specific reproductive antigens expressed at high levels in edible transgenic plant tissue might provide a safe, effective, and cheap oral delivery bait for immunocontraceptive control. as proof of concept, female possums vaccinated with immunocontraceptive an ... | 2005 | 15734054 |
| vap1, with cystatin c motif, an oocyte protein encoded by a novel ovarian-specific gene during oogenesis in the common brushtail possum (trichosurus vulpecula). | in the brushtail possum oocyte, vesicles accumulate in a polarized fashion at the vegetal pole and cytoplasm rich in mitochondria and containing the germinal vesicle comprise the animal pole. during cleavage to early blastocyst stages, animal pole cytoplasm locates to the cells of the embryonic hemisphere (pluriblast) and vegetal pole vesicular cytoplasm to cells of the abembryonic hemisphere (trophoblast). previously identified 16 amino acid residues, associated with the vesicle-rich cytoplasm ... | 2005 | 15736124 |
| experimental infection of possums with macropodid herpesvirus 1. | to determine whether macropodid herpesvirus 1 (mahv-1) could infect brushtail possums (trichosurus vulpecula) and whether such infection would lead to latency in possums. | 2004 | 15768078 |
| brushtail possums (trichosurus vulpecula) may pose a threat to public health in new zealand. | 2005 | 15782879 | |
| use of an electronic nose to diagnose mycobacterium bovis infection in badgers and cattle. | it is estimated that more than 50 million cattle are infected with mycobacterium bovis worldwide, resulting in severe economic losses. current diagnosis of tuberculosis (tb) in cattle relies on tuberculin skin testing, and when combined with the slaughter of test-positive animals, it has significantly reduced the incidence of bovine tb. the failure to eradicate bovine tb in great britain has been attributed in part to a reservoir of the infection in badgers (meles meles). accurate and reliable d ... | 2005 | 15814995 |
| unusually similar patterns of antibody v segment diversity in distantly related marsupials. | a pattern of coevolution between the v gene segments of ig h and l chains has been noted previously by several investigators. species with restricted germline v(h) diversity tend to have limited germline v(l) diversity, whereas species with high levels of germline v(h) diversity have more diverse v(l) gene segments. evidence for a limited pool of v(h) but diverse v(l) gene segments in a south american opossum, monodelphis domestica, is consistent with this marsupial being an exception to the pat ... | 2005 | 15843567 |
| conjunctival vaccination of the brushtail possum (trichosurus vulpecula) with bacille calmette-guérin. | to determine the efficacy of conjunctival vaccination of captive brushtail possums (trichosurus vulpecula) with bacille calmette-guérin (bcg), as measured by immunological responses to vaccination and response to intratracheal challenge with mycobacterium bovis. | 2005 | 15846397 |
| inheritance of resistance to mammalian herbivores and of plant defensive chemistry in an eucalyptus species. | hybridization in plants provides an opportunity to investigate the patterns of inheritance of hybrid resistance to herbivores, and of the plant mechanisms conferring this resistance such as plant secondary metabolites. we investigated how inter-race differences in resistance of eucalyptus globulus to a generalist mammalian herbivore, trichosurus vulpecula, are inherited in their fl hybrids. we assessed browsing damage of 3-year-old trees in a common environment field trial on four hybrid types o ... | 2005 | 15856789 |
| inheritance of resistance to mammalian herbivores and of plant defensive chemistry in a eucalyptus species. | hybridization in plants provides an opportunity to investigate the patterns of inheritance of hybrid resistance to herbivores, and of the plant mechanisms conferring this resistance such as plant secondary metabolites. we investigated how inter-race differences in resistance of eucalyptus globulus to a generalist mammalian herbivore, trichosurus vulpecula, are inherited in their f1 hybrids. we assessed browsing damage of three-year-old trees in a common environment field trial on four hybrid typ ... | 2005 | 15898499 |
| periodic fluid extrusion and models of digesta mixing in the intestine of a herbivore, the common brushtail possum (trichosurus vulpecula). | the digesta in four gut compartments (proximal and distal halves of small intestine, caecum, and proximal colon) of a wild hindgut fermenting herbivore, the common brushtail possum (trichosurus vulpecula), were investigated by rheometry and permeametry. digesta from all compartments were highly viscous and exhibited shear-thinning. apparent viscosity was positively related to dry matter content, and increased from proximal small intestine to colon. dynamic rheological measurements showed that in ... | 2005 | 15928916 |
| prolactin in the brushtail possum (trichosurus vulpecula): development of homologous radioimmunoassay using recombinant possum prolactin. | we report the production of recombinant possum prolactin (posprl), and its use in the development and validation of a highly specific homologous radioimmunoassay for the measurement of prolactin (prl) in brushtail possums. this enabled the subsequent investigation of some basic mechanisms involved in the regulation of prl secretion in this species. recombinant posprl spanning the entire coding region was expressed in escherichia coli, resulting in a 199 amino acid protein with a molecular weight ... | 2005 | 15935156 |
| susceptibility of brushtail possums (trichosurus vulpecula) infected with mycobacterium bovis is associated with a transient macrophage activation profile. | the australian brushtail possums are highly susceptible to mycobacterium bovis and are the principal wildlife reservoir of m. bovis in new zealand. to better understand the disease process in these animals, brushtail possums were infected by the aerosol route with a virulent strain of m. bovis, and immune parameters measured. m. bovis replicated actively in the lungs of infected animals. animals began developing macroscopic lung lesions at 4 and 5 weeks following infection, with some lesions app ... | 2005 | 15958259 |
| histology of the pouch epithelium and the mammary glands during chemically induced oestrus in the brushtail possum (trichosurus vulpecula). | changes in the epithelium of the maternal pouch and the mammary gland of brushtail possums (trichosurus vulpecula) were examined after animals were treated to induce ovulation with follicle-stimulating hormone (fsh), luteinizing hormone (lh), pregnant mares' serum gonadotrophin (pmsg) and oestradiol. the mammary glands were similar in appearance to those described in eutherian mammals and in previous studies on other marsupials. exposure of possums to these compounds, particularly pmsg, appeared ... | 2005 | 16011549 |
| failure to induce contact hypersensitivity in the opossum, trichosurus vulpecula. | 1982 | 16030830 | |
| cell-mediated immunity in opossums (trichosurus vulpecula): the responses to purified protein derivative and an unrelated antigen. | 1983 | 16030960 | |
| epidermal langerhans cells in the opossum, trichosurus vulpecula. | 2009 | 16031214 | |
| possums (trichosurus vulpecula) sharing dens: a potential infection route for bovine tuberculosis. | 1987 | 16031324 | |
| adenovirus precipitating antibodies in the sera of brush-tailed possums in new zealand. | sera collected from the australian brush-tailed possum trichosurus vulpecula in new zealand in 1975 and 1989 were tested for the presence of antibodies to adenovirus. of the 231 sera tested in an agar gel diffusion test, eight (3.5%) had precipitating antibodies to the group specific antigen of mammalian adenoviruses. available data allowed 99/231 sera to be classified as being obtained from either adult (total 62) or sexually immature (total 37) possums. from the adult animals, 4/62 (6.5%) sera ... | 1991 | 16031621 |
| giardia intestinalis in north island possums, house mice and ship rats. | giardia intestinalis cysts were detected in faeces from 12.9% of 124 brushtail possums (trichosurus vulpecula) trapped in auckland, hawke's bay, the wairarapa and wellington; in faecal material from 61.0% of 77 ship rats (rattus rattus) from areas of auckland, hawke's bay and wellington; and in faecal material from 25.3% of 182 house mice (mus musculus) from the north island. possums and rodents in forested areas of auckland and wellington city water catchments were infected. the role of wild an ... | 1992 | 16031653 |
| influence of stress of capture on haematological values and cellular immune responses in the australian brushtail possum (trichosurus vulpecula). | possums (trichosurus vulpecula) captured for the first time (single-capture) had significantly lower blood lymphocyte and eosinophil counts, but higher neutrophil counts than possums which had been captured frequently. single-capture possums also had depressed lymphocyte responses to concanavalin a (males and females) and pokeweed mitogen (males only). the haematological changes and the depressed lymphocyte reactivity to the t-cell mitogens were consistent with the animals being in a stressed st ... | 1992 | 16031681 |
| adiaspiromycosis in suspected cases of pulmonary tuberculosis in the common brushtail possum (trichosurus vulpecula). | pulmonary adiaspiromycosis was diagnosed in 15 of 17 common brushtail possums (trichosurus vulpecula) examined for bovine tuberculosis. emmonsia crescens was isolated from two of the affected animals. this is the first reported isolation of this dimorphic fungus in new zealand. the infections varied from light to heavy with sparsely distributed, focal, 1-2 mm diameter, grey-white spherical granulomas in the lightly infected cases to innumerable foci throughout the lungs in the heavily infected c ... | 1993 | 16031725 |
| lipopigments in the brushtail possum, trichosurus vulpecula. | two lipopigments have been found in the hepatic and lymphoid tissues of the brushtail possum, trichosurus vulpecula. one pigment was present in hepatocytes of five out of 15 (33%) possums from a site in the wairarapa region in the north island of new zealand, but was absent from the livers of 34 possums from a site in the manawatu. the pigment is possibly of dietary origin. an extra-hepatic lipopigment was found within macrophages in the lymph nodes of three out of 12 (25%) possums from the wair ... | 1994 | 16031749 |
| prevalence and spatial distribution of bovine tuberculosis in brushtail possums on a forest-scrub margin. | tuberculosis caused by mycobacterium bovis was diagnosed in 36 of 68 (53%) brushtail possums (trichosurus vulpecula) trapped in august 1992 from a population of exceptionally low density (trap catch <3%) on a forest-scrub margin in westland, new zealand. the prevalence of tuberculosis in possums, based solely on gross lesions, was at least twice that previously recorded in new zealand, and was about seven times that recorded from the same population in 1980. more male (66%) than female (33%) pos ... | 1994 | 16031762 |
| secondary phosphorus poisoning in dogs. | subsequent to a possum (trichosurus vulpecula) poisoning operation purportedly using 1% phosphorus baits, six dogs with access to poisoned possums died of phosphorus poisoning. two dogs survived, following treatment with oral copper sulphate and parenteral vitamin k. clinical signs included depression, jaundice, vomiting and bloody diarrhoea. post-mortem lesions included large areas of subcutaneous, interstitial and intermuscular haemorrhage, subserosal haemorrhage and liver degeneration. free p ... | 1995 | 16031797 |