Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| investigating the presence of organochlorine pesticides and polychlorinated biphenyls in wild and farmed gilthead sea bream (sparus aurata) from the western mediterranean sea. | the presence of organochlorine pesticides and polychlorinated biphenyls in wild and farmed gilthead sea bream (sparus aurata) from the western mediterranean, spain, is investigated. these pollutants were determined in white and red muscle and liver by gas chromatography coupled to tandem mass spectrometry. contaminant profiles present in both wild and farmed fish tissues include ddts, pcbs and hcb. the highest concentrations of contaminants were found in liver, and an increase in pollutant level ... | 2008 | 18289607 |
| linking the genomes of nonmodel teleosts through comparative genomics. | recently the genomes of two more teleost species have been released: the medaka (oryzias latipes), and the three-spined stickleback (gasterosteus aculateus). the rapid developments in genomics of fish species paved the way to new and valuable research in comparative genetics and genomics. with the accumulation of information in model species, the genetic and genomic characterization of nonmodel, but economically important species, is now feasible. furthermore, comparison of low coverage gene map ... | 2008 | 18297360 |
| copper transporter 1, metallothionein and glutathione reductase genes are differentially expressed in tissues of sea bream (sparus aurata) after exposure to dietary or waterborne copper. | the high affinity copper transporter 1 (ctr1), metallothionein (mt) and glutathione reductase (gr) are essential for copper uptake, sequestration and defense respectively. following rearing on a normal commercial diet (12.6+/-0.2 mg kg(-1) cu), sea bream were fed an experimental control diet lacking mineral mix (7.7+/-0.3 mg kg(-1) cu), an experimental diet enhanced with cu (135+/-4 mg kg(-1) cu) or an experimental diet (7.7+/-0.3 mg kg(-1) cu) whilst exposed to cu in water (0.294+/-0.013 mg l(- ... | 2008 | 18304880 |
| cyp27a1 expression in gilthead sea bream (sparus auratus, l.): effects of calcitriol and parathyroid hormone-related protein. | little is known about vitamin d metabolism in fishes. several reports have shown hydroxylase activities in various organs to produce vitamin d metabolites, but the enzymes involved have not been isolated or characterized. we isolated and characterized a renal mitochondrial hydroxylase, cyp27a1, that governs vitamin d metabolism in gilthead sea bream, sparus auratus. the enzyme is highly expressed in kidney and to a far lesser extent in liver. when treated with 25-hydroxy vitamin d or calcitriol, ... | 2008 | 18310458 |
| chronic exposure to the parasite enteromyxum leei (myxozoa: myxosporea) modulates the immune response and the expression of growth, redox and immune relevant genes in gilthead sea bream, sparus aurata l. | the myxosporean parasite enteromyxum leei invades the intestine of gilthead sea bream producing a slow-progressing disease, which may end in the death of fish. the present work aimed to better know the host immune response and the underlying molecular mechanisms, which may help to understand why some individuals seem to be refractory to the disease. three main aspects involved in fish health and welfare (immune, growth and redox status) were studied in fish exposed to e. leei-contaminated efflue ... | 2008 | 18343157 |
| evaluation of trace heavy metal levels of some fish species sold at retail in kayseri, turkey. | cd, ni, cr, zn, cu and pb concentrations of muscle tissue of 61 fish samples belonging to six fish species (sparus auratus, pomatomus saltatrix, sarda sarda, engraulis encrasicholus, sander lucioperca, scomber scombrus) retailed in kayseri, turkey were determined by flame atomic absorption spectrometry after wet digestion. cd concentrations of at least 31 fish samples (50.8%) and pb concentrations of at least six fish samples (9.8%) exceeded the corresponding turkish permissible limit of 0.05 an ... | 2009 | 18344008 |
| high levels of vegetable oils in plant protein-rich diets fed to gilthead sea bream ( sparus aurata l.): growth performance, muscle fatty acid profiles and histological alterations of target tissues. | the feasibility of fish oil (fo) replacement by vegetable oils (vo) was investigated in gilthead sea bream (sparus aurata l.) in a growth trial conducted for the duration of 8 months. four isolipidic and isoproteic diets rich in plant proteins were supplemented with l-lysine (0.55 %) and soya lecithin (1 %). added oil was either fo (control) or a blend of vo, replacing 33 % (33vo diet), 66 % (66vo diet) and 100 % (vo diet) of fo. no detrimental effects on growth performance were found with the p ... | 2008 | 18377678 |
| [aquaporins in gametogenesis of vertebrate animals]. | a review of the data on the presence, localization, and supposed role of aquaporin water channels in oocytes of xenopus laevis, oogenesis and maturation of teleosts sparus auratus and oncorhynchus mykiss, oogenesis and oocyte maturation of rats and mice, and spermatogenesis of several mammalians. | 2008 | 18409379 |
| molecular mechanisms determining sperm motility initiation in two sparids (sparus aurata and lithognathus mormyrus). | molecular mechanisms involved in sperm motility initiation in two sparids (sparus aurata and lithognathus mormyrus) have been studied. our comparative study demonstrates that osmolality is the key signal in sperm motility activation in both species, whereas k(+) and ca(2+) do not have any role. the straight-line velocity that resulted, however, was significantly different when measured in sperm activated with non-ionic and/or calcium-free solutions with respect to that measured in seawater-activ ... | 2008 | 18417709 |
| monospecies and multispecies probiotic formulations produce different systemic and local immunostimulatory effects in the gilthead seabream (sparus aurata l.). | the effects of the oral administration of heat-inactivated lactobacillus delbrüeckii ssp. lactis and bacillus subtilis, individually or combined, on gilthead seabream immune responses were investigated both systemically and locally in the gut. in a first experiment, seabream (65 g) were fed for 3 weeks different diets supplemented with 1 x 10(7)cfu g(-1)lactobacillus, 1 x 10(7)cfu g(-1)bacillus, or 0.5 x 10(7)cfu g(-1)lactobacillus plus 0.5 x 10(7)cfu g(-1)bacillus. controls were fed non-supplem ... | 2008 | 18442923 |
| ultrastructure of enteromyxum leei (diamant, lom, & dyková, 1994) (myxozoa), an enteric parasite infecting gilthead sea bream (sparus aurata) and sharpsnout sea bream (diplodus puntazzo). | the ultrastructure of the developmental stages of the myxozoan enteromyxum leei parasitizing gilthead seabream (sparus aurata) intestine and sharpsnout sea bream (diplodus puntazzo) intestine and gallbladder are described. the earliest stage observed was a small dense trophozoite located among enterocytes. proliferative stages, observed intercellularly in the epithelium of the intestine and gallbladder as well as in the lumen, possessed the typical cell-in-cell configuration throughout their dev ... | 2008 | 18460155 |
| effects on growth and biochemical responses in juvenile gilthead seabream 'sparus aurata' after long-term dietary exposure to low levels of dioxins. | the farmed fish gilthead seabream (sparus aurata) were fed with a dry feed spiked with a low level (23 ng who-teq/kg of feed) polychlorinated dibenzo-p-dioxins and dibenzofurans (pcdd/fs) mixture in order to assess bioaccumulation of these contaminants in the muscle and liver tissues after long-term exposure (approximately 390 days). furthermore, effects on fish growth, feeding and on the response of some biochemical markers (induction of the cyp1a dependent erod activity, the conjugating enzyme ... | 2008 | 18472132 |
| purification and kinetic characterization of 6-phosphofructo-1-kinase from the liver of gilthead sea bream (sparus aurata). | 6-phosphofructo-1-kinase (pfk) was purified to homogeneity from liver of gilthead sea bream (sparus aurata) and kinetic properties of the enzyme were determined. the native enzyme had an apparent molecular mass of 510 kda and was composed of 86 kda subunits, suggesting homohexameric structure. at ph 7, s. aurata liver pfk (pfkl) showed sigmoidal kinetics for fructose-6-phosphate (fru-6-p) and hyperbolic kinetics for atp. fructose-2,6-bisphosphate (fru-2,6-p2) converted saturation curves for fru- ... | 2008 | 18483063 |
| rearing temperature enhances hepatic glucokinase but not glucose-6-phosphatase activities in european sea bass (dicentrarchus labrax) and gilthead sea bream (sparus aurata) juveniles fed with the same level of glucose. | the aim of this work was to elucidate if the previous results observed in hepatic glucokinase (gk) and glucose-6-phosphatase (g6pase) activities in european sea bass and gilthead sea bream are due to temperature per se or to differences in feed intake at different water temperatures. for that purpose triplicate groups of fish (30 g initial body weight) were kept at 18 degrees c or 25 degrees c during two weeks and fed a fixed daily ration of a glucose-free or 20% glucose diet. at the end of the ... | 2008 | 18508397 |
| molecular characterization of interleukin-6 in the gilthead seabream (sparus aurata). | a cdna clone, designated sbil-6 (seabream interleukin-6), was obtained from a cdna library of enriched immune-stimulated sequences from gilthead seabream. the deduced sbil-6 protein corresponds to a 225-amino acid protein with a putative 24-amino acid signal peptide, four conserved alpha helices and one n-linked glycosylation site. at the amino acid level sbil-6 shares 23-26% identity with mammalian il-6 sequences and 30-51% identity with other fish il-6 sequences. the structure of the sbil-6 ge ... | 2008 | 18513800 |
| the expression profile of tlr9 mrna and cpg odns immunostimulatory actions in the teleost gilthead seabream points to a major role of lymphocytes. | the potential effects of synthetic unmethylated oligodeoxynucleotides (odn) containing cpg motifs, mimicking bacterial dna, has never been evaluated on the immune response in the teleost fish gilthead seabream (sparus aurata), the most important fish species in mediterranean aquaculture. first, binding and competition studies have demonstrated that binding is saturated and promiscuous, suggesting the participation of several receptors. moreover, leucocyte cytotoxic (ncc) activity, production of ... | 2008 | 18535781 |
| cadmium accumulation and biochemical responses in sparus aurata following sub-lethal cd exposure. | cadmium (cd), a heavy metal with limited biological function, is widely distributed in the aquatic environment as a result of natural and anthropogenic activities. the effect of 4 and 11 days exposure of gilthead sea bream sparus aurata to sub-lethal concentrations of cd was evaluated as levels of cd content and cd-metallothionein (mt) presence in different organs. the possible genotoxic effect was also evaluated in erythrocytes by using the "comet assay", a promising tool for estimating dna dam ... | 2009 | 18538842 |
| a novel alternatively spliced transcript of cytosolic alanine aminotransferase gene associated with enhanced gluconeogenesis in liver of sparus aurata. | increased alanine aminotransferase (alt) activity is associated with insulin resistance and the development of type 2 diabetes. the aim of this study was to characterize the modulation of cytosolic alt expression in liver of gilthead sea bream (sparus aurata) under conditions associated with increased gluconeogenesis and in streptozotocin (stz)-treated fish. rt- and race-pcr assays allowed us to isolate a novel alt isozyme (calt2) generated from alternative splicing of calt gene in s. aurata. he ... | 2008 | 18582594 |
| effect of water temperature and dietary starch on growth and metabolic utilization of diets in gilthead sea bream (sparus aurata) juveniles. | we evaluated the effect of dietary starch level on growth performance, feed utilization, whole-body composition and activity of selected key enzymes of intermediary metabolism in gilthead sea bream juveniles reared at 18 and 25 degrees c. a diet was formulated to contain 48% crude protein, 12% lipids and 30% gelatinized maize starch (diet 30gs). two other diets were formulated to include the same level of ingredients as diet 30gs except for the gelatinized starch, which was included at 20% (diet ... | 2008 | 18586542 |
| histopathological changes caused by enteromyxum leei infection in farmed sea bream sparus aurata. | histological examinations were carried out on the stomach, pyloric caeca and 4 different parts of the intestine, as well as the rectum, hepatopancreas, gall bladder and spleen of 52 sea bream sparus aurata spontaneously infected by enteromyxum leei. fifteen fish from a non-infected farm were included as a control. clinical signs appeared only in extensively and severely infected fish. we observed enteromyxum leei almost exclusively in the intestinal tract, and very rarely in the intrahepatic bil ... | 2008 | 18589998 |
| cardicola aurata sp. n. (digenea: sanguinicolidae) from mediterranean sparus aurata l. (teleostei: sparidae) and its unexpected phylogenetic relationship with paradeontacylix mcintosh, 1934. | a new sanguinicolid trematode, cardicola aurata sp. n., is described from gilthead seabream sparus aurata l., from off the spanish mediterranean coast. the morphology of c. aurata sp. n. generally agrees with the diagnosis of the genus, however, in contrast to all other reported cardicola spp. the male pore is located sub-medially at the posterior end of the body instead of sinistrally before the posterior end of the body. based on a comparison of the morphology as well as partial 28s and its2 r ... | 2008 | 18639649 |
| nk-like and oxidative burst activities are the main early cellular innate immune responses activated after virus inoculation in reservoir fish. | viral diseases are a major problem in fish farming and a deeper knowledge of the immunological mechanisms playing a part in the antiviral defence is still important. moreover, fish farming practices (high densities, new areas of culture and egg/larvae/adult transport) are significantly increasing the spread of viruses and the number of susceptible or reservoir fish species. in this last point, no studies have focused on the immunological mechanisms playing a part in the antiviral responses in re ... | 2008 | 18657621 |
| acute effects of cadmium on liver phase i and phase ii enzymes and metallothionein accumulation on sea bream sparus aurata. | this research was designed to study sparus aurata (sea bream) biotransformation and detoxification responses to acute exposure to cadmium (cd). sexually immature gilthead sea bream were treated by intraperitoneal injection of cd chloride (200 microg kg(-1)) for 6, 12, 24 and 48 h. cd accumulation was quantified in sea bream liver by graphite furnace atomic absorption spectroscopy after the various exposure periods. the following biological responses were measured: (1) ethoxyresorufin-o-deethylas ... | 2008 | 18665457 |
| effect of cryopreservation on phosphorylation state of proteins involved in sperm motility initiation in sea bream. | we have previously demonstrated that in sea bream sparus aurata motility initiation determined changes in the phosphorylation state of some proteins. this paper describes an investigation of the effect of a freezing-thawing procedure on the protein phosphorylation/dephosphorylation pattern. proteins extracted from fresh and cryopreserved spermatozoa (before and after motility activation) were separated on sds-page, blotted on nitrocellulose membrane and treated with anti-phosphotyrosine, anti-ph ... | 2008 | 18700136 |
| effects of the organochlorines p,p'-dde and lindane on gilthead seabream leucocyte immune parameters and gene expression. | toxicological effects of pesticides in water-living animals are very important, especially when these animals are for human consumption. thus, in vitro tests were performed to evaluate the immunotoxicological impact of two organochlorines, lindane and p,p'-dde, on gilthead seabream (sparus aurata l.) leucocytes, an economically important fish-farmed species in the mediterranean area. leucocyte viability (apoptosis and necrosis), innate immune parameters (phagocytosis, respiratory burst and cell- ... | 2008 | 18757214 |
| seasonal trends and tissue distribution of organochlorine pollutants in wild and farmed gilthead sea bream (sparus aurata) from the western mediterranean sea and their relationship with environmental and biological factors. | seasonal trends and tissue distribution of organochlorine pesticides (ocps) and polychlorinated biphenyls (pcbs) in gilthead sea bream (sparus aurata) samples from the western mediterranean were investigated. hexane extracts of white and red muscle, liver, and gills from wild and cultured specimens were purified by normal-phase high-performance liquid chromatography, and selected ocps and pcbs were determined by gas chromatography coupled to mass spectrometry as indicators of contamination. the ... | 2009 | 18779940 |
| light synchronization of the daily spawning rhythms of gilthead sea bream (sparus aurata l) kept under different photoperiod and after shifting the ld cycle. | reproduction in most fish is typically a seasonal process, as spawning takes place usually at a given time of the year, depending on the reproduction strategy of the species, to ensure maximal survival of offspring. nevertheless, fish reproduction cannot be considered an exclusively annual phenomenon, because spawning may also show daily rhythmicity. in this study, we investigated the existence of a daily spawning rhythm in gilthead seabream (sparus aurata l) exposed to different light-dark (ld) ... | 2008 | 18780197 |
| polymerase chain reaction amplification of repetitive intergenic consensus and repetitive extragenic palindromic sequences for molecular typing of pseudomonas anguilliseptica and aeromonas salmonicida. | the aim of this study was to evaluate the use of two molecular techniques, repetitive extragenic palindromic polymerase chain reaction (rep-pcr) and repetitive intergenic consensus pcr (eric-pcr), as epidemiological tools with which to discriminate among genetically distinct strains within two bacterial fish pathogens, pseudomonas anguilliseptica and aeromonas salmonicida. a total of 30 a. salmonicida and 52 p. anguilliseptica were analyzed. for p. anguilliseptica, three different major fingerpr ... | 2008 | 18783128 |
| application of in situ detection techniques to determine the systemic condition of lymphocystis disease virus infection in cultured gilt-head seabream, sparus aurata l. | immunohistochemistry (ihc) and in situ hybridization (ish) techniques have been used for the detection of lymphocystis disease virus (lcdv) in formalin-fixed, paraffin-embedded tissues from gilt-head seabream, sparus aurata l. diseased and recovered fish from the same population were analysed. ihc was performed with a polyclonal antibody against a 60-kda viral protein. a specific digoxigenin-labelled probe, obtained by pcr amplification of a 270-bp fragment of the gene coding the lcdv major caps ... | 2009 | 18803582 |
| structural and functional divergence of two fish aquaporin-1 water channels following teleost-specific gene duplication. | teleost radiation in the oceans required specific physiological adaptations in eggs and early embryos to survive in the hyper-osmotic seawater. investigating the evolution of aquaporins (aqps) in these vertebrates should help to elucidate how mechanisms for water homeostasis evolved. the marine teleost gilthead sea bream (sparus aurata) has a mammalian aquaporin-1 (aqp1)-related channel, termed aqp1o, with a specialized physiological role in mediating egg hydration. however, teleosts have an add ... | 2008 | 18811940 |
| effect of in vitro exposure to vibrio vulnificus on hydroelectrolytic transport and structural changes of sea bream (sparus aurata l.) intestine. | the everted gut sac technique has been used to investigate the effect of vibrio vulnificus on water and electrolyte (na(+), k(+), cl(-), hco(3)(-)) transport on the intestine of sea bream (sparus aurata l.). both the anterior and the posterior intestine were incubated in a medium containing 10(8) v. vulnificus cells ml(-1) at 25 degrees c for 2 h. the presence of v. vulnificus resulted in a significant reduction (p < 0.05) of water absorption in the anterior intestine, while sodium absorption in ... | 2009 | 18825505 |
| molecular modelling of co-receptor cd8 alpha alpha and its complex with mhc class i and t-cell receptor in sea bream (sparus aurata). | t-cells are the main actors of cell-mediated immune defence; they recognize and respond to peptide antigens associated with mhc class i and class ii molecules. in this paper, we investigated by molecular modelling methods in the teleost sea bream (sparus aurata) the interaction among the molecules of the tertiary complex cd8/mhc-i/tcr, which determines the t-cell-mediated immunological response to foreign molecules. first, we predicted the three-dimensional structure of cd8 alpha alpha dimer and ... | 2008 | 18951040 |
| immunohistochemical detection of estrogen receptors in fish scales. | calcium mobilization from internal stores, such as scales, induced by 17beta-estradiol during sexual maturation in salmonids is well documented. this calcium mobilization from scales is proposed to be mediated by the estrogen receptor (er). however, the er subtypes involved and signaling mechanisms responsible for this effect remain to be fully characterized. in the present study, we have localized eralpha, erbetaa and erbetab proteins in juvenile and adult sea bream (sparus auratus) and mozambi ... | 2009 | 18977356 |
| viral encephalopathy and retinopathy of dicentrarchus labrax and sparus aurata farmed in tunisia. | viruses belonging to the nodaviridae family cause disease worldwide among a large number of species of marine fish, and have been described in all continents. in the present study, a total of 69 farmed tunisian sea bass (dicentrarchus labrax) and 24 sea bream (sparus aurata) samples were tested monthly for the detection of betanodavirus. the virus was identified in both species using indirect immunofluorescence assays (ifat) and rt-pcr. in addition sequence analysis of part of the coat protein g ... | 2009 | 18982421 |
| oral delivery of live yeast debaryomyces hansenii modulates the main innate immune parameters and the expression of immune-relevant genes in the gilthead seabream (sparus aurata l.). | microorganisms isolated from fish can be used as prophylactic tools for aquaculture in the form of probiotic preparations. the purpose of this study was to evaluate the effects of dietary administration of the live yeast debaryomyces hansenii cbs 8339 on the gilthead seabream (sparus aurata l.) innate immune responses. seabream were fed control or d. hansenii-supplemented diets (10(6) colony forming units, cfu g(-1)) for 4 weeks. humoral (seric alternative complement and peroxidase activities), ... | 2008 | 19004644 |
| classification accuracy of algorithms for blood chemistry data for three aquaculture-affected marine fish species. | the objective of this study was determination and discrimination of biochemical data among three aquaculture-affected marine fish species (sea bass, dicentrarchus labrax; sea bream, sparus aurata l., and mullet, mugil spp.) based on machine-learning methods. the approach relying on machine-learning methods gives more usable classification solutions and provides better insight into the collected data. so far, these new methods have been applied to the problem of discrimination of blood chemistry ... | 2009 | 19031001 |
| development and validation of a gene expression oligo microarray for the gilthead sea bream (sparus aurata). | aquaculture represents the most sustainable alternative of seafood supply to substitute for the declining marine fisheries, but severe production bottlenecks remain to be solved. the application of genomic technologies offers much promise to rapidly increase our knowledge on biological processes in farmed species and overcome such bottlenecks. here we present an integrated platform for mrna expression profiling in the gilthead sea bream (sparus aurata), a marine teleost of great importance for a ... | 2008 | 19055773 |
| some organochlorine pesticide residues in fish species in konya, turkey. | the levels of organochlorine pesticides were measured in 18 fish species from konya markets, turkey. these species were selected on the basis of their importance to local human fish consumption. the extracted residues were analyzed on a micro capillary gas chromatograph equipped with an electron capture detector. total 14 different organochlorine pesticides were determined. these residues were detected in all fish species, except in trout, horse mackerel and bonito. ddt and its metabolites and h ... | 2009 | 19103455 |
| adhesive properties of environmental vibrio alginolyticus strains to biotic and abiotic surfaces. | the ability of vibrio alginolyticus strains isolated from a bathing and fishing area (khenis, centre of tunisia) to adhere to both biotic and abiotic surfaces was evaluated in the present work. the biochemical, physiological and enzymatic activities of all strains was also investigated. three morphotypes of v. alginolyticus were obtained on congo red agar and only 14 strains produced black colonies. the majority of strains were able to degrade the skin mucus of both sparus aurata and dicentrarch ... | 2008 | 19123304 |
| spermiogenic nuclear protein transitions and chromatin condensation. proposal for an ancestral model of nuclear spermiogenesis. | we have chosen three species (sparus aurata, dicentrarchus labrax, and monodonta turbinata) that represent different transition patterns in the composition and structure of spermiogenic nuclei. the transition patterns of these species are representative of spermiogenesis in a large number of animal species. we analyze: (a) nuclear protein exchange; (b) chromatin condensation pattern; and (c) histone acetylation during spermiogenic development. in the simplest spermiogenesis histones and nucleoso ... | 2009 | 19132734 |
| biochemical response in gilthead seabream (sparus aurata) to in vivo exposure to a mix of selected pahs. | in our previous studies we assessed the biochemical response of juvenile seabream to the exposure to three selected polycyclic aromatic hydrocarbons (pahs) (phenanthrene, pyrene and fluorene) in short-term laboratory experiments. since in the analyses conducted in the field it is much more natural to expect the presence of multiple contaminants at the same time, the objective of this study was to analyse the reaction of seabream to the exposure to a mix of the three pahs tested previously. the p ... | 2009 | 19167757 |
| evolution of lipopolysaccharide (lps) recognition and signaling: fish tlr4 does not recognize lps and negatively regulates nf-kappab activation. | it has long been established that lower vertebrates, most notably fish and amphibians, are resistant to the toxic effect of lps. furthermore, the lack of a tlr4 ortholog in some fish species and the lack of the essential costimulatory molecules for lps activation via tlr4 (i.e., myeloid differentiation protein 2 (md-2) and cd14) in all the fish genomes and expressed sequence tag databases available led us to hypothesize that the mechanism of lps recognition in fish may be different from that of ... | 2009 | 19201835 |
| high potential of adhesion to abiotic and biotic materials in fish aquaculture facility by vibrio alginolyticus strains. | the ability of vibrio alginolyticus strains isolated from sparus aurata and dicentrarchus labrax nursery to adhere to epithelial cell lines (hep-2 and caco-2), fish mucus and their ability to form a biofilm on different surfaces (glass, polystyrene, polyethylene and polyvinyl-chloride) was investigated in this study. | 2009 | 19245411 |
| gene and protein expression for prolactin, growth hormone and somatolactin in sparus aurata: seasonal variations. | the seasonal variation of prl, gh and sl gene and protein expression has been analyzed in gilthead sea bream (sparus aurata) pituitaries using real-time q-pcr and western blots, respectively. animals were cultured in earthen ponds under natural photoperiod, temperature and salinity conditions. samples were taken during winter 2005 (january), spring 2005 (april), summer 2005 (july) and autumn 2005 (october). beta-actin, used as the housekeeping gene both for q-rt-pcr and western analysis, did not ... | 2009 | 19250974 |
| a yeast assay based on the gilthead sea bream (teleost fish) estrogen receptor beta for monitoring estrogen mimics. | a yeast (saccharomyces cerevisiae)-based assay was developed and tested with steroids and chemicals (mostly pesticides). the induction of beta-galactosidase activity was strictly dependent on the presence of seabream (sparus aurata) betaa estrogen receptor (sberbetaa) and substances known to have estrogenic activity. 17beta-estradiol (e(2)) and diethylstilbestrol (des), both agonists, were most active and the antagonist tamoxifen (tam) was 14-fold less active than e(2). among the chemicals teste ... | 2009 | 19303142 |
| [strong genetic differentiation of the gilthead sea bream sparus aurata (l., 1758) between the two western banks of the mediterranean]. | we characterised four samples of gilthead sea bream from the two western mediterranean banks with three microsatellite loci and two rapds systems. contrarily to what could be predicted for a highly mobile species with a planktonic larval dispersal phase, we observed a strong and significant genetic differentiation at all loci between the two banks (f(st)=0.069(***)), whereas two samples from the gulf of lions were almost identical (f(st)=0.003 ns) while the two from the gulf of annaba displayed ... | 2009 | 19304263 |
| description of enterovibrio nigricans sp. nov., reclassification of vibrio calviensis as enterovibrio calviensis comb. nov. and emended description of the genus enterovibrio thompson et al. 2002. | eleven strains of halophilic, facultative anaerobes isolated from healthy and diseased dentex dentex and sparus aurata (bony fishes) cultured in spanish mediterranean fisheries have been studied by a polyphasic approach that included a wide phenotypic characterization, dna-dna hybridization and phylogenetic analysis using 16s rrna, reca and rpod gene sequences. all strains were phylogenetically related to enterovibrio species and vibrio calviensis. on the basis of sequence analysis and dna-dna h ... | 2009 | 19329591 |
| isolation and characterization of toll-like receptor 9 in half-smooth tongue sole cynoglossus semilaevis. | toll-like receptors (tlrs) are considered as key sensors to trigger the host's innate immune system and adaptive immune responses by recognizing various pamps and initiating signal transduction. tlr9, as a member of tlr family, mediates the recognition of unmethylated cpg dinucleotide motifs commonly found in both bacterial and viral genomes. in the current study, the tlr9 gene was isolated from one of flatfish species, half-smooth tongue sole (cynoglossus semilaevis). in the 4588 bp genomic seq ... | 2009 | 19340952 |
| seasonality of nuclear abnormalities in gilthead sea bream sparus aurata (l.) erythrocytes. | the frequencies of erythrocyte nuclear abnormalities, such as irregularly shaped (isn), vacuolated nuclei (vn), binucleated cells (bn), including micronuclei (mn), were monitored in the peripheral blood of cultured gilthead sea bream (sparus aurata l.) from the southern adriatic sea (croatia) and assessed for a relation to seasonality. fish were sampled in february, april, june, and september. the lowest frequencies of isn and mn were measured in april, and the lowest frequencies of vn and bn we ... | 2009 | 19343523 |
| acute effects of benzo[a]pyrene on liver phase i and ii enzymes, and dna damage on sea bream sparus aurata. | in the present study biotransformation and detoxification responses to acute exposure to the polycyclic aromatic hydrocarbons benzo[a]pyrene (b[a]p) were investigated in the liver of sparus aurata (sea bream). sexually immature gilthead sea bream were treated by intraperitoneal injection of b[a]p (20 mg kg(-1)) for 6, 12, 24, and 48 h. b[a]p accumulation was quantified in sea bream liver by mean of gas phase chromatography (gpc-ms) after the various exposure periods. the following biological res ... | 2009 | 19343524 |
| expression of pituitary prolactin, growth hormone and somatolactin is modified in response to different stressors (salinity, crowding and food-deprivation) in gilthead sea bream sparus auratus. | prolactin (prl), growth hormone (gh) and somatolactin (sl) expression was studied in gilthead sea bream (sparus auratus) in response to several different stressors (salinity, food deprivation or stocking density). in the first experiment, specimens were acclimated during 100 days at three different environmental salinities: low salinity water (lsw, 6 ppt), brackish water (bw, 12 ppt) and seawater (sw, 38 ppt). osmoregulatory parameters corresponded to those previously reported for this species u ... | 2009 | 19348804 |
| profiling of infection specific mrna transcripts of the european seabass dicentrarchus labrax. | the european seabass (dicentrarchus labrax), one of the most extensively cultured species in european aquaculture productions, is, along with the gilthead sea bream (sparus aurata), a prospective model species for the perciformes which includes several other commercially important species. massive mortalities may be caused by bacterial or viral infections in intensive aquaculture production. revealing transcripts involved in immune response and studying their relative expression enhances the und ... | 2009 | 19361338 |
| disruption of the thyroid system by diethylstilbestrol and ioxynil in the sea bream (sparus aurata). | some environmental contaminants are thought to cause disruption of the thyroid system in vertebrates acting as endocrine disrupting chemicals (edcs). such chemicals may affect synthesis, transport and metabolism of thyroid hormones (ths). ioxynil (iox) and diethylstilbestrol (des) are potential edcs with strong affinity in vitro for sea bream transthyretin (ttr), a th distributor protein (thdp). the aim of the present study was to establish how such chemicals influence the thyroid axis in sea br ... | 2009 | 19375178 |
| comparison of biochemical composition of three aqua cultured fishes (dicentrarchus labrax, sparus aurata, dentex dentex). | the moisture, ash, protein and fat content and amino acid, fatty acid and mineral compositions in the flesh of three commercially important fish species (dicentrarchus labrax, sparus aurata, dentex dentex) from the aqua culture of turkey were evaluated. ranges of moisture, ash, protein and fat contents were 69.68-76.42%, 1.49-1.95%, 18.21-21.70% and 2.29-8.10%, respectively. among them, those occurring in the highest proportions were aspartic acid (3238.91-5204.71 mg/100 g), glutamic acid (5256. ... | 2008 | 19382346 |
| gyrodactylus orecchiae sp. n. (monogenea: gyrodactylidae) from farmed populations of gilthead seabream (sparus aurata) in the adriatic sea. | gyrodactylus orecchiae sp. n. (monogenea, gyrodactylidae) is described from the skin, fins, eyes and gills of juvenile sparus aurata l. (gilthead seabream) following two outbreaks of gyrodactylosis amongst stocks held in inshore floating cages on the adriatic coast of albania and croatia. fish were heavily infected (1000+ gyrodactylids/fish) with g. orecchiae which reportedly resulted in approximately 2-10% mortality amongst the infected stock. morphologically, the haptoral hooks of g. orecchiae ... | 2009 | 19391328 |
| evaluation of candidate reference genes for qpcr during ontogenesis and of immune-relevant tissues of european seabass (dicentrarchus labrax). | the expression level of mrna can vary significantly in different experimental conditions, such as stress, infection, developmental stage or tissue. suitable reference genes are expected to exhibit constant expression levels. however no single gene is constitutively expressed in all cell types and under all experimental conditions. it has become clear that expression stability of the intended reference gene has to be examined before each experiment. for expression studies using quantitative real- ... | 2009 | 19398033 |
| effects of feeding schedule on locomotor activity rhythms and stress response in sea bream. | feeding cycles entrain biological rhythms, which enable animals to anticipate feeding times and so maximize food utilization and welfare. in this article, the effect of mealtime was investigated in two groups of sea bream (sparus aurata): one group received a single daily meal at random times during the light period (random daytime feeding, rdf), whereas the other group received the meal during the light period but at the same time (scheduled daytime feeding, sdf). all the fish showed diurnal be ... | 2009 | 19410591 |
| heavy metal bioaccumulation and metallothionein content in tissues of the sea bream sparus aurata from three different fish farming systems. | the distribution and potential bioaccumulation of dietary and waterborne cadmium and lead in tissues of sea bream (sparus aurata), a major aquaculture species, was studied in relation to three different fish farming systems. metallothionein levels in fish tissues were also evaluated. results demonstrate that metal concentrations in various tissues significantly vary among fish culture systems. different tissues show different capacity for accumulating heavy metals. the content of both cadmium an ... | 2010 | 19444632 |
| effects of fish oil replacement and re-feeding on the bioaccumulation of organochlorine compounds in gilthead sea bream (sparus aurata l.) of market size. | organochlorine pesticide residues and polychlorinated biphenyls were determined in raw materials, fish feeds and fillets from fish exposed through the productive cycle (14 months) to experimental diets with different percentages of fish oil replacement with vegetable oils. detectable amounts of organochlorine compounds were found in raw materials derived from fish sources with none being detected in vegetable ingredients. fish feeds presented trace concentrations of contaminants at the ng/g leve ... | 2009 | 19450868 |
| stimulation of gut immune system by early administration of probiotic strains in dicentrarchus labrax and sparus aurata. | early feeding (started during gut metamorphosis and establishment of the hypothalamic-pituitary-interrenal axis) with probiotic-supplemented diets, besides modifying the intestinal microflora, evoked profound effects on the physiology of fish larvae. using rotifers and artemia as living vectors, the autochthonous bacterium lactobacillus delbrueckii delbrueckii or a multispecies probiotic formulation (autochthonous lactobacillus fructivorans+lactobacillus plantarum from human feces) were orally a ... | 2009 | 19456355 |
| invasion and survival of photobacterium damselae subsp. piscicida in non-phagocytic cells of gilthead sea bream, sparus aurata l. | fluorescence microscopy and gentamicin protection assays were used to investigate the ability of four photobacterium damselae subsp. pisicida (phdp) strains to adhere to and to invade the fish epithelial cell line, saf-1, derived from sparus aurata. all strains tested were detected intracellularly using both techniques, although internalization levels varied among strains. treatment with cytochalasin d and experiments carried out at 4 degrees c demonstrated that a functional host cell cytoskelet ... | 2009 | 19460085 |
| dynamics of liver gh/igf axis and selected stress markers in juvenile gilthead sea bream (sparus aurata) exposed to acute confinement: differential stress response of growth hormone receptors. | the time courses of liver gh/igf axis and selected stress markers were analyzed in juvenile gilthead sea bream (sparus aurata) sampled at zero time and at fixed intervals (1.5, 3, 6, 24, 72 and 120 h) after acute confinement (120 kg/m(3)). fish remained unfed throughout the course of the confinement study, and the fasting-induced increases in plasma growth hormone (gh) levels were partially masked by the gh-stress inhibitory tone. hepatic mrna levels of growth hormone receptor-i (ghr-i) were not ... | 2009 | 19524697 |
| coordination of deiodinase and thyroid hormone receptor expression during the larval to juvenile transition in sea bream (sparus aurata, linnaeus). | to test the hypothesis that ths play an important role in the larval to juvenile transition in the marine teleost model, sea bream (sparus auratus), key elements of the thyroid axis were analysed during development. specific rt-pcr and taqman quantitative rt-pcr were established and used to measure sea bream iodothyronine deiodinases and thyroid hormone receptor (tr) genes, respectively. expression of deiodinases genes (d1 and d2) which encode enzymes producing t3, trs and t4 levels start to inc ... | 2010 | 19549532 |
| targets for tnfalpha-induced lipolysis in gilthead sea bream (sparus aurata l.) adipocytes isolated from lean and fat juvenile fish. | the present study aimed to analyze adiposity heterogeneity and the role of liver x receptor (lxralpha) and peroxisome proliferator-activated receptors (ppars) as targets of tumour necrosis factor-alpha (tnfalpha) in gilthead sea bream (sparus aurata l.). the screening of 20 fish at the beginning of the warm season identified two major groups with fat and lean phenotypes. fat fish showed increased liver and mesenteric fat depots. this increased adiposity was concurrent in the adipose tissue to en ... | 2009 | 19561215 |
| evidence for the involvement of aquaporins in sperm motility activation of the teleost gilthead sea bream (sparus aurata). | the expression of aquaporins in the spermatozoa of the marine teleost gilthead sea bream (sparus aurata) and their involvement in the motility activation process were investigated. sperm motility was activated by a hyperosmotic shock, but it was completely inhibited by 10 microm hgcl(2), such inhibition being partially recovered by beta-mercaptoethanol (me). conventional rt-pcr using primers specific for s. aurata aquaglyceroporin (glp) and aquaporin 1a (aqp1a) demonstrated the presence of both ... | 2009 | 19571262 |
| cytochrome p450 (cyp1a) induction in sea bream (sparus aurata) gills and liver following exposure to polychlorobiphenyls (pcbs). | 2009 | 19588260 | |
| experimental transmission of sparicotyle chrysophrii (monogenea: polyopisthocotylea) to gilthead seabream (sparus aurata) and histopathology of the infection. | the polyopisthocotylean sparicotyle chrysophrii (van beneden et hesse, 1863) was experimentally transmitted to gilthead seabream (sparus aurata l.) by exposure to eggs (egt) and by cohabitation with naturally parasitized fish (ct). in egt trials, the infection was successfully transmitted by introducing containers with monogenean eggs in the fish tanks, with the highest infection level (85.7% prevalence, 3.3 mean intensity) achieved at 6 weeks post exposure (p.e.) to the infection dose of 650 eg ... | 2009 | 19606789 |
| pituitary gene and protein expression under experimental variation on salinity and temperature in gilthead sea bream sparus aurata. | temperature and salinity are important factors that affect several physiological processes in aquatic organisms, which could be produced by variation of certain hormones. in this study, the expression of pituitary hormones involved in the acclimation to different temperatures and salinities was examined in sparus aurata, a euryhaline and eurytherm species, by q-real time rt-pcr and western blot analyses for mrna and protein expression, respectively. three different experimental conditions were d ... | 2009 | 19607931 |
| evaluation of the possible transmission of bse and scrapie to gilthead sea bream (sparus aurata). | in transmissible spongiform encephalopathies (tses), a group of fatal neurodegenerative disorders affecting many species, the key event in disease pathogenesis is the accumulation of an abnormal conformational isoform (prp(sc)) of the host-encoded cellular prion protein (prp(c)). while the precise mechanism of the prp(c) to prp(sc) conversion is not understood, it is clear that host prp(c) expression is a prerequisite for effective infectious prion propagation. although there have been many stud ... | 2009 | 19636413 |
| interactive effects of environmental salinity and temperature on metabolic responses of gilthead sea bream sparus aurata. | the gilthead sea bream sparus aurata is a euryhaline and euritherm species with the capacity of living under different environmental conditions of salinity and temperature. the influence of acclimation to different environmental salinities (5, 38 and 55 per thousand) and temperatures (12 degrees , 19 degrees and 26 degrees c) for seven weeks was analyzed in plasma and tissues (liver, gills and kidney) of gilthead sea bream assessing levels of metabolites and enzyme activities related to energy m ... | 2009 | 19651228 |
| comparing the inhibitory effects of five protoxicant organophosphates (azinphos-methyl, parathion-methyl, chlorpyriphos-methyl, methamidophos and diazinon) on the spontaneously beating auricle of sparus aurata: an in vitro study. | organophosphates (ops) can provoke toxicity by inhibiting acetylcholinesterase (ache) in non-target organisms, like fish. in a previous pilot study, the anticholinesterase effects of paraoxon on the heart of sparus aurata were examined [tryfonos, m., antonopoulou, e., papaefthimiou, c., chaleplis, g., theophilidis, g., 2009. an in vitro assay for the assessment of the effects of an organophosphate, paraoxon, and a triazine, atrazine, on the heart of the gilthead sea bream (sparus aurata). pest. ... | 2009 | 19674799 |
| metals in tissues of fish from yelkoma lagoon, northeastern mediterranean. | this study was performed to investigate the metal concentrations in muscle, liver, gonad, and gill of gilthead seabream (sparus aurata l., 1758), european seabass (dicentrarchus labrax l., 1758), and keeled mullet (liza carinata valenciennes, 1836) from yelkoma lagoon, northeastern mediterranean region. so, the levels of cadmium, cobalt, chromium, copper, iron, manganese, nickel, lead, and zinc in tissues of specimens from the lagoon were determined by inductively coupled plasma optical emission ... | 2010 | 19680760 |
| anandamide and am251, via water, modulate food intake at central and peripheral level in fish. | the endocannabinoid system is a major regulator of food intake in many animal species. studies conducted so far have mostly focused on mammals, and, therefore, in this study, the role of the endocannabinoid system in food intake in the sea bream sparus aurata was investigated. the effect of different doses of the endocannabinoid anandamide (aea), administered via water, was evaluated after different exposure times (30, 60 and 120 min) at both physiological and molecular levels. the results obtai ... | 2010 | 19800340 |
| impact of an acoustic stimulus on the motility and blood parameters of european sea bass (dicentrarchus labrax l.) and gilthead sea bream (sparus aurata l.). | the physiological responses of fish to underwater noise are poorly understood and further information is needed to evaluate any possible negative effects of sound exposure. we exposed european sea bass and gilthead sea bream to a 0.1-1 khz linear sweep (150 db(rms) re 1 micropa). this band frequency is perceptible by many species of fish and is mainly produced by vessel traffic. we assessed the noise-induced motility reaction (analysing the movements) and the haematological responses (measuring ... | 2010 | 19828191 |
| fast screening immunoassay of sulfonamides in commercial fish samples. | an indirect competitive enzyme-linked immunosorbent assay (elisa) was developed in plate to detect three sulfonamide residues (sulfamerazine (smr), sulfadimetoxine (sdm), and sulfadiazine (sdz)) in gilthead sea bream (sparus aurata) samples. different extraction methodologies--using methanol/water 1:1 (v/v) + ethylene diamine tetraacetic acid (edta) 0.5% (m/v), acetonitrile, phosphate-buffered saline (pbs) 10 mmol l(-1) ph 7 and acetate buffer 100 mmol l(-1) ph 5--and cleanup steps, based on sol ... | 2010 | 19882144 |
| growth performance and metabolic utilization of diets including starch, dextrin, maltose or glucose as carbohydrate source by gilthead sea bream (sparus aurata) juveniles. | the effect of dietary carbohydrate complexity on growth, feed utilization and activity of selected key liver enzymes of intermediary metabolism were studied in gilthead sea bream juveniles. four isonitrogenous (50% crude protein) and isolipidic (16% crude lipids) diets were formulated to contain 20% of pregelatinized maize starch, dextrin, maltose or glucose. triplicate groups of fish (117 g initial weight) were fed each diet to near satiation during 6 weeks. no effect of dietary carbohydrate on ... | 2010 | 19894133 |
| investigation of several virulence properties among vibrio alginolyticus strains isolated from diseased cultured fish in tunisia. | we analysed 34 vibrio alginolyticus strains isolated from gilthead sea bream sparus aurata l. and sea bass dicentrarchus labrax l. cultured in fish farms on the tunisian mediterranean coast for the presence of several virulence properties such as extracellular products (ecp) production, growth in iron-limiting conditions and survival in fish serum. the results obtained with different substrates showed that ecp of v. alginolyticus were hydrolytic. the virulence was correlated with the ability of ... | 2009 | 19899346 |
| nutritional fatty acid quality of raw and cooked farmed and wild sea bream (sparus aurata). | the effects of steaming, grilling, and frying in corn and sunflower oils, respectively, on the fatty acid compositions of farmed and wild sea bream were evaluated. the lipid content increased with frying in both oil types. the maximum moisture value was found in steamed fish (p<0.05). fried sea bream in corn and sunflower oils contained a lower content of n-3 polyunsaturated fatty acids (p<0.05) (3.87 and 5.32% of total fatty acids (tfa) in farmed fish and 2.96 and 2.14% tfa in wild fish). the n ... | 2010 | 19911842 |
| detection of antimicrobial peptides related to piscidin 4 in important aquacultured fish. | epithelial surfaces of fish, such as the gut, skin and gills, comprise a large surface area for possible pathogen invasion. antimicrobial peptides (amps), innate immunity components, play a significant role in protecting fish. piscidins are a family of amps. in this study, we detected the presence of the recently discovered piscidin 4 via bug blot, western blot, elisa and/or immunohistochemistry in striped bass (morone saxatilis), white bass (m. chrysops), european seabass (dicentrarchus labrax) ... | 2010 | 19913049 |
| comparative toxicity of cadmium in the commercial fish species sparus aurata and solea senegalensis. | the induction of metallothionein-like proteins (mtlps) as well as cadmium levels (cd) was studied in tissues of gilthead sea bream sparus aurata and flatfish solea senegalensis after an intraperitoneal (i.p.) injection of cadmium (2.5 mg kg(-1) body weight). the liver, gills, intestine and blood of s. aurata as well as the liver and intestine of s. senegalensis were collected for analysis at 0, 3 and 6 days after the injection. cd levels significantly increased in all tissues of the treated anim ... | 2010 | 19913912 |
| synchronization to light and restricted-feeding schedules of behavioral and humoral daily rhythms in gilthead sea bream (sparus aurata). | food is not continuously available in the wild, and so most animals show a wide variety of circadian rhythms that can be entrained to feeding time. the aim of this research was to evaluate the effect of time-restricted feeding on the daily rhythms of gilthead sea bream, with food being provided during the day or night under a 12:12 h light-dark (ld) cycle or constant light (ll) conditions. self-feeding and locomotor activity, as well as daily rhythms of cortisol, glucose, and melatonin, were eva ... | 2009 | 19916838 |
| energy reserves and metabolic status affect the acclimation of gilthead sea bream (sparus aurata) to cold. | during winter, low temperatures induce a direct metabolic depression in gilthead sea bream, without any significant compensatory effect below 13 degrees c. the present study therefore focused on how to improve response to cold in these fish, looking specifically at the two factors of diet (high energy, hie, and low energy, loe) and activity (normal, -sw, and sustained activity, +sw) prior to exposure to cold. following a preparatory period of 75 days water was adjusted to 10 degrees c and kept f ... | 2010 | 19931633 |
| accumulation of some heavy metals seasonally in hysterotylacium aduncum (nematoda) and its host red sea bream, pagellus erythrinus (sparidae) from gulf of iskenderun (north-eastern mediterranean). | the red sea bream's nematode and sparus aurata, sampled from the iskenderun bay, north-eastern mediterranean in march 2008 were analysed by inductively coupled plasma-atomic emission spectrometry (icp-aes) for their some heavy metal (cd, cr, cu, fe, hg, mn, mg, pb and zn) levels. the metal concentrations of the parasites were compared to different organs (liver, muscle, swimbladder, intestine and skin) of the fish hosts. the highest cd (0.303 mg/kgg ww) concentrations were found in the muscle, h ... | 2010 | 19946663 |
| bioaccumulation of polycyclic aromatic hydrocarbons in gilthead sea bream (sparus aurata l.) exposed to long term feeding trials with different experimental diets. | polycyclic aromatic hydrocarbons (16 epa list) were determined in oils, fish feed, and fillets from gilthead sea bream fed through a full production cycle (14 months) with feed containing different proportions of fish oil replaced by vegetable oils, followed by a finishing phase with fish oil. at the beginning of the study, fish presented 46.6 microg/kg fresh weight of the sum of pahs in fillet and a benzo[a]pyrene equivalent value of 9.1 microg/kg fresh weight. these levels decreased after 330 ... | 2010 | 20037783 |
| multiple cu-atpase genes are differentially expressed and transcriptionally regulated by cu exposure in sea bream, sparus aurata. | copper (cu) is an essential metal, although in excess is highly toxic due to its redox properties and, therefore intracellular cu homeostasis is a highly regulated process. cu-atpases are pivotal regulatory, proteins of intracellular and bodily cu homeostasis. two cu-atpases, atp7a and atp7b with distinct, functions are found in mammals and herein we report the structure and expression under cu stress of, homologues of atp7a and atp7b in gilthead sea bream (sparus aurata), the first such report ... | 2010 | 20044148 |
| gene expression survey of mitochondrial uncoupling proteins (ucp1/ucp3) in gilthead sea bream (sparus aurata l.). | the aim of this work is to underline the biological significance of mitochondrial uncoupling proteins (ucps) in ectothermic fish using the gilthead sea bream (sparus aurata l.) as an experimental model. a contig of 1,990 bp in length was recognized as a ucp1 ortholog after initial searches in the gilthead sea bream aquafirst database ( http://www.sigenae.org/aquafirst ). additional searches were performed in skeletal muscle by rt-pcr, and the amplified pcr product was recognized as ucp3 after se ... | 2010 | 20063001 |
| tissue-specific robustness of fatty acid signatures in cultured gilthead sea bream (sparus aurata l.) fed practical diets with a combined high replacement of fish meal and fish oil. | the present study aimed to determine the tissue-specific robustness of fatty acid (fa) signatures of gilthead sea bream (sparus aurata l.) by analyzing the changes in lipid class and fa composition of skeletal muscle, brain, liver, and mesenteric adipose tissue. triplicate groups of fish were fed to visual satiety over a 14-mo production cycle with 2 practical plant protein-based diets formulated with either fish oil or a blend of vegetable oils (66% of fish oil replacement) to contain 53% cp an ... | 2010 | 20081079 |
| cloning of wap65 in sea bass (dicentrarchus labrax) and sea bream (sparus aurata) and expression in sea bass tissues. | the complementary dna encoding wap65 protein was cloned from the liver of two fish species sea bass (dicentrarchus labrax) and sea bream (sparus aurata). full-length cdna sequences were obtained from reverse transcribed total rna, followed by 5' and 3' rapid amplification of cdna end (race) experiments. the full-length cdna sequence of d. labrax is 1709bp and the coding sequence is flanked by a 67bp 5'-utr and a 358bp 3'-utr. the full-length cdna sequence of s. aurata is 1599bp, and the coding s ... | 2010 | 20085823 |
| identification of six novel cc chemokines in gilthead seabream (sparus aurata) implicated in the antiviral immune response. | chemokines are key regulators of migration and consequent activation of migrating leukocytes. cc chemokines constitute the largest chemokine group with 24-28 members in mammalian species, and even more in teleost fish, with up to 81 members in zebrafish danio rerio. further studies concerning fish chemokine genes will help elucidate the complexity of this chemokine group which has considerably expanded in some teleosts. in the current work, we have identified six novel cc chemokine genes within ... | 2010 | 20096460 |
| lectinhistochemical detection of terminal carbohydrate residues in the enteric myxozoan enteromyxum leei parasitizing gilthead seabream sparus aurata (pisces: teleostei): a study using light and transmission electron microscopy. | the presence of terminal carbohydrate residues in enteromyxum leei (diamant, lom et dyková, 1994) palenzuela, redondo et alvarez-pellitero, 2002 stages in gilthead seabream intestines was studied at light microscopy (lm) and transmission electron microscopy (tem) level using lectin histochemical techniques. abundant mannose and/or glucose residues were demonstrated by the intense staining caused by binding of biotinylated concanavalin a (con a), at both lm and tem. a clear positivity was also ob ... | 2009 | 20128238 |
| identification of vibrio harveyi as a causative bacterium for a tail rot disease of sea bream sparus aurata from research hatchery in malta. | a bacterial disease was reported from gilthead sea bream (sparus aurata) within a hatchery environment in malta. symptoms included complete erosion of tail, infection in the eye, mucous secretion and frequent mortality. a total of 540 strains were initially isolated in marine agar from different infected body parts and culture water sources. subsequently 100 isolates were randomly selected, identified biochemically and all were found to be vibrio harveyi-related organisms; finally from 100 isola ... | 2010 | 20129765 |
| nucleotide sequence, tissue expression patterns and phylogenetic analysis of estrogen receptor one mrna in the murray rainbowfish (melanotaenia fluviatilis) (atheriniformes, actinopterygii). | estrogens are steroidal hormones that control many physiological processes in both female and male vertebrates. like other vertebrates, fish have two distinct estrogen receptors (esr) subtypes, esr1 and esr2a that have been isolated in a number of species, as well as a third subtype, esr2b. the mrna encoding the esr1 was isolated from the female liver of an australian freshwater fish, the murray rainbowfish, melanotaenia fluviatilis. the rainbowfish esr1 cdna was 2569 bp in length and with an op ... | 2010 | 20171224 |
| crtac1 homolog proteins are conserved from cyanobacteria to man and secreted by the teleost fish pituitary gland. | cartilage acidic protein 1 (crtac1) gene expression is used as a marker for chondrocyte differentiation in stem cell-based tissue engineering. it is also transcribed outside the skeleton where at least two different transcripts are expressed in lung and brain. in the pituitary gland of the teleost fish sea bream sparus auratus, we have found a transcript with a high degree of sequence identity to crtac1 family members but lacking the egf-like calcium-binding domain encoding sequence of crtac1 an ... | 2010 | 20171266 |
| immunochemical determination of oxytetracycline in fish: comparison between enzymatic and time-resolved fluorometric assays. | an indirect competitive enzyme-linked immunosorbent assay (elisa) with photometric detection of horseradish peroxidase (hrp) activity, was developed in plate to detect oxytetracycline (otc) in gilthead sea bream (sparus aurata) samples. the results were compared to those obtained by time-resolved fluoroimmunoassay (tr-fia) using a secondary antibody with coproporphyrin of platinum (ii) (ptcp) as marker. the limits of detection obtained in fish extract were 16 and 0.08 microg kg(-1) for photometr ... | 2010 | 20171317 |
| molecular cloning and characterization of adult sparus aurata hemoglobin genes. | among teleosts, sparus aurata occupies a prominent place in the gastronomic and economic fields of the mediterranean basin and other geographic districts. the knowledge of its molecular structures and functional features, such as hemoglobin, may be helpful to understand the adaptive biochemical mechanisms that allow this fish to live under extreme conditions, including fish farming. in sparus aurata red blood cells two different alpha and one beta hemoglobin genes have been identified. the alpha ... | 2010 | 20210659 |
| the essential and toxic elements in tissues of six commercial demersal fish from eastern mediterranean sea. | the essential elements and contaminants (pb and cd) were determined seasonally in the muscle and liver of six demersal fish species sparus aurata, chelidonichthys lucernus, upeneus molluccensis, solea solea, merluccius merluccius and saurida undosquamis from the iskenderun bay, eastern mediterranean sea. the concentrations in muscles of fish species were 474-1534 mg/kg for na, 574-3374 mg/kg for k, 72.7-496 m/kg ca, 94.1-210 mg/kg for mg. muscle accumulated the lowest levels of elements. trace e ... | 2010 | 20214948 |
| use of microarray technology to assess the time course of liver stress response after confinement exposure in gilthead sea bream (sparus aurata l.). | selection programs for growth and stress traits in cultured fish are fundamental to the improvement of aquaculture production. the gilthead sea bream (sparus aurata) is the main aquacultured species in the mediterranean area and there is considerable interest in the genetic improvement of this species. with the aim of increasing the genomic resources in gilthead sea bream and identifying genes and mechanisms underlying the physiology of the stress response, we developed a cdna microarray for gil ... | 2010 | 20307314 |
| prl and gh synthesis and release from the sea bream (sparus auratus l.) pituitary gland in vitro in response to osmotic challenge. | the endocrine factors prolactin (prl) and growth hormone (gh) are believed to have counteracting effects in the adaption of fish to changes in environmental salinity. in order to further investigate this interaction sea bream were challenged with full seawater (sw) or freshwater (fw) for 7 days and the response of pituitary glands cultured in vitro to an osmotic challenge (230, 275 and 320 mosm/kg) was assessed. in vitro prl secretion from pituitaries of sw-adapted fish was unaltered in response ... | 2010 | 20406642 |
| parathyroid hormone-related protein-stanniocalcin antagonism in regulation of bicarbonate secretion and calcium precipitation in a marine fish intestine. | bicarbonate secretion in the intestine (duodenum) of marine fish has been suggested to play a major role in regulation of calcium availability for uptake. however, while the end process may lead to carbonate precipitation, regulation of transport of calcium and/or bicarbonate may actually result in fine-tuning of calcium availability for transport. to test this hypothesis, sea bream (sparus auratus) duodenal preparations were mounted in ussing-type chambers and the effect of parathyroid hormone- ... | 2010 | 20410471 |
| sparicotyle chrysophrii (van beneden and hesse 1863) (monogenea: polyopisthocotylea) parasite of cultured gilthead sea bream sparus aurata (linnaeus 1758) (pisces: teleostei) from corsica: ecological and morphological study. | sparicotyle chrysophrii infections were highlighted during a parasitological monitoring carried out on cultured gilthead sea bream sparus aurata linnaeus 1758 from aquaculture sites in corsica. factors and biological mechanisms regulating parasites populations, particularly controlling the infestation and survival of monogeneans have been studied. variations in infection support the hypotheses that the main factor determining the infection of s. chrysophrii was temperature and that the parasite ... | 2010 | 20422218 |