Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| [the nature of melanin pigments from some micro- and macromycetes]. | new inhibitors of melanin formation by micromycetes aspergillus carbonarius, alternaria alternata, and paecilomyces variotii and basidiomycetes inonotus obliquus and phellinus robustus were found. precursors of melanin pigments were isolated and identified. p-hydroxybenzoic acid was identified among the products of alkaline degradation of melanin formed by micromycetes, whereas in the case of macromycetes this was protocatechuic acid. therefore, melanins of the former were found to belong to the ... | 2002 | 12068581 |
| copper complexes with bioactive ligands. part ii--antifungal activity. | antifungal activity of new copper(ii) complexes of 2-methylthionicotinate (2-mesnic) of the composition cu(2-mesnic)2(menia)(2).4h2o (where menia is n-methylnicotinamide), and cu(2-mesnic)2(nia)(2).2h2o (where nia is nicotinamide) and cu(2-mesnic)2l2 (where l is isonicotinamide, inia, or ethyl nicotinate, etnic) were tested on various strains of filamentous fungi by the macrodilution method. most sensitive against copper(ii) adducts with bioactive ligands were rhizopus oryzae and microsporum gyp ... | 2002 | 12094729 |
| allergenic cross-reactivity of curvularia lunata with other airborne fungal species. | curvularia lunata is an important fungus for respiratory allergic disorders. previous studies indicated cross-reactivity of curvularia with other fungi. however, the cross-reactive allergenic component (s) were not identified. the present work was carried out to study the shared allergenic components of c. lunata and others. | 2002 | 12100306 |
| the allergens of cladosporium herbarum and alternaria alternata. | 2002 | 12102004 | |
| protective effect of tenuazonic acid against dimethyl benz(a)antracene-induced skin carcinogenesis in mice. | anticarcinogenic potential of tenuazonic acid (ta), a mycotoxin isolated from a fungus, alternaria alternata, on skin tumorigenesis induced by 7,12-dimethyl benz(a) antracene (dmba) was investigated. female swiss albino mice were exposed topically to 100 nmole of dmba twice weekly for 20 weeks. another group of animals was treated with 250 microg ta in acetone daily for a period of 1 week, followed by the same dose of ta prior to every application of dmba. at the end of 14 weeks, all the animals ... | 2002 | 12111715 |
| high yield synthesis of tentoxin, a cyclic tetrapeptide. | tentoxin is a naturally occurring phytotoxic cyclic tetrapeptide excreted by fungi of the alternaria alternata family. the four total syntheses of tentoxin published to date give poor total yields, mainly owing to two difficulties, the introduction of the dehydro amino acid and more especially the cyclization step. here we describe a method that stereospecifically introduces z-dehydrophenylalanine (deltazphe) by a modified erlenmeyer aldolization reaction. the linear tetrapeptide, boc-r1ala-leu- ... | 2002 | 12148783 |
| ph regulates endoglucanase expression and virulence of alternaria alternata in persimmon fruit. | the phytopathogenic fungus alternaria alternata produces one endo-1,4-beta-glucanase, aak1, which is an important factor in disease development in persimmon fruit. during growth of a. alternata in media containing acidified yeast extract or cell walls from persimmon fruit, the fungus secreted ammonia and raised the medium ph. a rise in media ph from 3.8 to 6.0 in the presence of cell walls induced the expression of aak1, whereas a glucose-induced decline in ph to 2.5 repressed transcription and ... | 2002 | 12182334 |
| sensitisation to airborne moulds and severity of asthma: cross sectional study from european community respiratory health survey. | to assess whether the severity of asthma is associated with sensitisation to airborne moulds rather than to other seasonal or perennial allergens. | 2002 | 12193354 |
| evidence for the genetic control of immunoglobulin e reactivity to the allergens of alternaria alternata. | the fungus alternaria alternata contains potent allergens, and sensitization to these allergens is associated with a high risk of respiratory disease. the influence of genetic regulation on sensitization to alternaria is unknown. | 2002 | 12220470 |
| fumonisin- and aal-toxin-induced disruption of sphingolipid metabolism with accumulation of free sphingoid bases. | fumonisins (fb) and aal-toxin are sphingoid-like compounds produced by several species of fungi associated with plant diseases. in animal cells, both fumonisins produced by fusarium moniliforme and aal-toxin produced by alternaria alternata f. sp. lycopersici inhibit ceramide synthesis, an early biochemical event in the animal diseases associated with consumption of f. moniliforme-contaminated corn. in duckweed (lemna pausicostata heglem. 6746), tomato plants (lycopersicon esculentum mill), and ... | 1994 | 12232389 |
| detection of alternaria fungal contamination in cereal grains by a polymerase chain reaction-based assay. | alternaria sp. are important fungal contaminants of grain products; they secrete four structural classes of compounds that are toxic or carcinogenic to plants and animals and cause considerable economic losses to growers and the food-processing industry. alternaria toxins have been detected by high-performance liquid chromatography (hplc), enzyme-linked immunosorbent assay, and other techniques. here, we report the development of a polymerase chain reaction (pcr)-based method for the detection o ... | 2002 | 12233854 |
| apoptosis: a functional paradigm for programmed plant cell death induced by a host-selective phytotoxin and invoked during development. | the host-selective aal toxins secreted by alternaria alternata f sp lycopersici are primary chemical determinants in the alternaria stem canker disease of tomato. the aal toxins are members of a new class of sphinganine analog mycotoxins that cause cell death in both animals and plants. here, we report detection of stereotypic hallmarks of apoptosis during cell death induced by these toxins in tomato. dna ladders were observed during cell death in toxin-treated tomato protoplasts and leaflets. t ... | 1996 | 12239387 |
| antigenic and allergenic cross-reactivity of epicoccum nigrum with other fungi. | previous studies have identified epicoccum nigrum (en) as an important aeroallergen. shared allergenicity among some fungi responsible for type i allergic disorders has been reported. | 2002 | 12269649 |
| cross-reactivity between raw mushroom and molds in a patient with oral allergy syndrome. | oral allergy syndrome, resulting from a cross-reactivity between raw fruits and vegetables and a number of pollens, is well described. however, it has never been associated with mold spore sensitivity and mushrooms. we evaluated a patient with oral allergy symptoms to raw, but not cooked, mushrooms, who also had positive skin testing to molds. | 2002 | 12269655 |
| constitutive expression of a celery mannitol dehydrogenase in tobacco enhances resistance to the mannitol-secreting fungal pathogen alternaria alternata. | our previous observation that host plant extracts induce production and secretion of mannitol in the tobacco pathogen alternaria alternata suggested that, like their animal counterparts, plant pathogenic fungi might produce the reactive oxygen quencher mannitol as a means of suppressing reactive oxygen-mediated plant defenses. the concurrent discovery that pathogen attack induced mannitol dehydrogenase (mtd) expression in the non-mannitol-containing host tobacco suggested that plants, unlike ani ... | 2002 | 12366799 |
| expression of the antiapoptotic baculovirus p35 gene in tomato blocks programmed cell death and provides broad-spectrum resistance to disease. | the sphinganine analog mycotoxin, aal-toxin, induces a death process in plant and animal cells that shows apoptotic morphology. in nature, the aal-toxin is the primary determinant of the alternaria stem canker disease of tomato, thus linking apoptosis to this disease caused by alternaria alternata f. sp. lycopersici. the product of the baculovirus p35 gene is a specific inhibitor of a class of cysteine proteases termed caspases, and naturally functions in infected insects. transgenic tomato plan ... | 2002 | 12403830 |
| characterization of enolase allergen from rhodotorula mucilaginosa. | rhodotorula mucilaginosa (also known as r. rubra) is among the most commonly found yeast strains in our environment. however, allergens from r. mucilaginosa have not yet been characterized at the molecular level. the purpose of this study was to characterize the enolase allergen from r. mucilaginosa and examine the allergenic/antigenic cross-reactivity among fungal enolases. the full-length cdna encoding the r. mucilaginosa enolase was isolated through the reverse transcriptase-polymerase chain ... | 2002 | 12432231 |
| diversity of microfungi in the phylloplane of plants growing in a mediterranean ecosystem. | mediterranean ecosystems have not been investigated as natural habitats for microorganisms in general, and microfungi in particular. plants harvested in "serra da arrábida" (38 degrees 27' n, 9 degrees 02' w), a mediterranean ecosystem in portugal, were analyzed for the filamentous microfungi inhabiting their surface. two field locations with distinct climatic characteristics were studied: 'fonte do veado' (38 degrees 28'50" n, 9 degrees 0'17" w; 300 m elevation) located on the northern slope, a ... | 2002 | 12442302 |
| the plant disease resistance gene asc-1 prevents disruption of sphingolipid metabolism during aal-toxin-induced programmed cell death. | the nectrotrophic fungus alternaria alternata f.sp. lycopersici infects tomato plants of the genotype asc/asc by utilizing a host-selective toxin, aal-toxin, that kills the host cells by inducing programmed cell death. asc-1 is homologous to genes found in most eukaryotes from yeast to humans, suggesting a conserved function. a yeast strain with deletions in the homologous genes lag1 and lac1 was functionally complemented by asc-1, indicating that asc-1 functions in an analogous manner to the ye ... | 2002 | 12445127 |
| a diterpene quinone from the bark of cryptomeria japonica. | a diterpene, cryptoquinone, was isolated from the bark of cryptomeria japonica, the structure, 7,11,14-trioxoabieta-8,12-diene, was established by spectral analyses and x-ray crystallography. this diterpene quinone showed moderate antifungal activities against pyricularia orizae and alternaria alternata, and cytotoxic activity against mouse lymphoid neoplasm (p388) cells with an ic(50) of 0.26 microg/ml. | 2002 | 12453513 |
| development of a selective myclobutanil agar (mba) medium for the isolation of fusarium species from asparagus fields. | a new selective myclobutanil agar medium for the detection of fusarium, species is proposed. ten media formulations based on various selective agents (pentachloronitrobenzene (pcnb), rose bengal, malachite green, sodium hypochlorite, captan, benomyl, chlorotalonil, myclobutanil, thiram, and cupric sulfate) were compared. first, mycelium growth and colony appearance of alternaria alternata, aspergillus flavus, cladosporium cladosporioides, epicoccum nigrum, fusarium sp., fuisarium solani, fusariu ... | 2002 | 12455616 |
| signal transduction in lemon seedlings in the hypersensitive response against alternaria alternata: participation of calmodulin, g-protein and protein kinases. | the development of an effective hypersensitive response (hr) in any plant system relies, not only in their gene composition and expression, but also on an effective and rapid signal transduction system. lemon seedlings induce the phenylpropanoid pathway, which results in the de novo biosynthesis of the phytoalexin scoparone, as part of the hypersensitive response against alternaria alternata. in order to elucidate some of the signaling elements that participate in the development of hr in lemon ... | 2002 | 12462990 |
| [immunotherapy with an oral alternaria extract in childhood asthma. clinical safety and efficacy and effects on in vivo and in vitro parameters]. | studies of immunotherapy with oral alternaria extracts are scarce. we decided to perform a clinical trial of the clinical safety and efficacy of this extract as well as of its effects on in vivo and in vitro parameters in 39 patients with alternaria allergy, aged between 7 and 17 years, who are also sensitized extract was used. allergic activity was determined through rast inhibition and skin prick test. quantification of the principal allerten (alt a 1) was performed through the 2-site binding ... | 2002 | 12464165 |
| case report. keratomycosis due to alternaria alternata in a diabetic patient. | a case of keratitis caused by alternaria alternata in a diabetic male, after traumatic corneal injury with a sharp object, is described. the patient was treated with topical amphotericin b solution and a full uneventful recovery was achieved. this is the first reported case of fungal keratamycosis in malta, which was substantiated with both positive direct microscopy and a positive culture of the fungus. | 2002 | 12472731 |
| sem study of water activity and temperature effects on the initial growth of aspergillus ochraceus, alternaria alternata and fusarium verticillioides on maize grain. scanning electron microscopy. | a scanning electron microscopy (sem) study of the effect of water activity (a(w)), temperature and fungal interactions on the very early phases of aspergillus ochraceus, alternaria alternata and fusarium verticillioides development on maize grains was carried out. germination and growth of individual fungal strains were assayed at 0.92, 0.95 and 0.98 a(w), and 20 and 30 degrees c. hyphal lengths were measured on micrographs taken by sem at different periods of incubation. a. alternata had the hi ... | 2003 | 12485744 |
| cloning of brn1, a reductase gene involved in melanin biosynthesis in cochliobolus heterostrophus. | the plant pathogenic fungus cochliobolus heterostrophus produces melanin, a black pigment, via 1, 8-dihydroxynaphthalene. the deficiency of c. heterostrophus brn1(-) mutant was complemented with the cosmid clone pcos/ml6, screened by heterologous hybridization with the genes involved in the melanin biosynthesis of alternaria alternata. we determined the dna sequence of the brn1 gene and its flanking regions. the brn1 gene contains one open reading frame consisting of three exons separated by two ... | 1997 | 12501329 |
| isolation of fungi in musca domestica linnaeus, 1758 (diptera: muscidae) captured at two natural breeding grounds in the municipality of serop dica, rio de janeiro, brazil. | the objective of this study was to isolate and identify fungal species found in natural association with adults of musca domestica. the adult insects were collected from two natural breeding grounds: hog pens and an urban sanitary landfill. the isolated fungi were identified as: aspergillus flavus (23.8%), a. niger var. niger (14.4%), penicillium corylophilum (21.4%), p. fellutanum (11.9%), cladosporium cladosporoides (4.7%), fusarium sp. (4.7%), alternaria alternata (11.9%), curvularia brachysp ... | 2003 | 12563474 |
| molecular taxonomy of the alternaria and ulocladium species from humans and their identification in the routine laboratory. | the alternaria and ulocladium species reported from humans are studied taxonomically using rdna internal transcribed spacer (its) sequence data. the its variability within the genus is relatively limited. the two most important, longicatenate species, alternaria alternata and a. infectoria, clearly differ in their its domains, due to a 26-bp insert in its1 of the latter species. a number of taxa inhabiting particular plant species, such as a. longipes on tobacco and a. mali on apple, but also th ... | 2002 | 12572714 |
| case report. cutaneous phaeohyphomycosis due to alternaria alternata. | a case of cutaneous alternariosis with a well-delimited lesion of traumatic origin is described in a renal transplant recipient. on the basis of histopathology the case was first thought to be cryptococcosis, but alternaria alternata was identified after culturing by means of morphological and molecular examination. surgical treatment, accompanied by prophylactic application of itraconazole (200 mg day(-1) for 4 weeks), resulted in complete cure. | 2002 | 12572725 |
| familial and environmental determinants for wheezing and asthma in a case-control study of school children in palestine. | our prevalence study on palestinian school children aged 6-12 years showed lower rates for asthma and asthma symptoms than economically developed and industrialized countries. reasons for such differences are largely unknown, and could possibly be related to different environmental and lifestyle factors. | 2003 | 12580909 |
| allergen detection from 11 fungal species before and after germination. | allergens dispersed by airborne fungal spores play an important but poorly understood role in the underlying cause and exacerbation of asthma. previous studies suggest that spores of alternaria and aspergillus release greater quantities of allergen after germination than before germination. it is unknown whether this is true of other allergenic fungi. | 2003 | 12589346 |
| antifungal activity of biflavones from taxus baccata and ginkgo biloba. | bilobetin and 4'''-o-methylamentoflavone were isolated and identified in the needles of taxus baccata, for the first time in this species. the antifungal activity of biflavones from t. baccata and ginkgo biloba, namely amentoflavone, 7-o-methylamentoflavone, bilobetin, ginkgetin, sciadopitysin and 2,3-dihydrosciadopitysin towards the fungi alternaria alternata, fusarium culmorum, cladosporium oxysporum was determined employing computer-aided image analysis couplet to a microscope. bilobetin exhi ... | 2003 | 12622229 |
| hypersensitivity to molds in new york city in adults who have asthma. | molds have been linked epidemiologically to asthma as a key aeroallergen in several studies. other allergens such as cockroach have been linked to asthma in new york city (nyc). to our knowledge, however, the pattern of mold hypersensitivity has never been examined systematically in the nyc area. thus, we sought to determine the association between mold hypersensitivity and asthma in a large group of ambulatory patients evaluated for allergic disease for the years 1993 through 2001 at a single m ... | 2003 | 12635573 |
| nuclear transport factor 2 represents a novel cross-reactive fungal allergen. | ubiquitously occuring moulds are important allergenic sources known to elicit ige-mediated allergic diseases and to share cross-reactive allergens. limited information is available about the molecular structures involved in cross-reactivity. we aimed to clone and characterize cross-reactive mould allergens. | 2003 | 12653793 |
| purification and characterization of a cross-reactive 45-kd major allergen of fusarium solani. | fusarium solani (fs) is an important source of fungal allergen. a 45-kd major allergen of fs showed reactivity with patients' sera sensitive to many fungi. | 2003 | 12660423 |
| ige binding synthetic peptides of alt a 1, a major allergen of alternaria alternata. | alternaria alternata protein, alt a 1 is a major allergen associated with allergy in atopic patients. although the molecule binds strongly to ige antibody from patients, the epitopes involved have not been identified or defined. in the present study, we synthesized overlapping peptides spanning the whole sequence and evaluated their ige binding with sera from patients with alternaria-induced allergy. the results identified four ige binding linear regions. two of these regions k41-p50 and y54-k63 ... | 2003 | 12668200 |
| cloning of a gene encoding an alt a 1 isoallergen differentially expressed by the necrotrophic fungus alternaria brassicicola during arabidopsis infection. | alternaria species are considered some of the most important fungi responsible for allergenic morbidity in humans. the alternaria protein that elicits the most intense allergic reaction in humans is alt a 1, yet no biological function has been identified for this protein. in this study, suppression subtractive hybridization and virtual northern blots were used to identify and characterize an alt a 1 homolog in the phytopathogenic fungus alternaria brassicicola. rna was extracted from a. brassici ... | 2003 | 12676721 |
| removal of phenanthrene from soil by co-cultures of bacteria and fungi pregrown on sugarcane bagasse pith. | sixteen co-cultures composed of four bacteria and four fungi grown on sugarcane bagasse pith were tested for phenanthrene degradation in soil. the four bacteria were identified as pseudomonas aeruginose, ralstonia pickettii, pseudomonas sp. and pseudomonas cepacea. the four fungi were identified as: penicillium sp., trichoderma viride, alternaria tenuis and aspergillus terrus that were previously isolated from different hydrocarbon-contaminated soils. fungi had a statistically significant positi ... | 2003 | 12699938 |
| role of reactive oxygen species and antioxidants in plant disease resistance. | membrane damage caused by the non-specific fungal toxin fusaric acid was less on pretreated than on control leaves when tobacco leaves were pretreated with anti-senescence plant hormones, such as kinetin, benzyladenine or the anti-ozonant n-[2-(2-oxo-1-imidazolidinyl)]ethyl-n'-phenylurea. similarly, the necrosis caused by mercuric chloride was reduced by the above anti-senescence agents. in addition, in in vitro tests, leaves from selected paraquat-tolerant tobacco plants were less sensitive to ... | 2003 | 12701708 |
| inhibitory activity of analogs of am-toxin, a host-specific phytotoxin from the alternaria alternata apple pathotype, on photosynthetic o2 evolution in apple leaves. | the effect of the host-specific phytotoxins, am-toxins, on the photosynthetic activity of leaves from susceptible apple cultivars was investigated by using an oxygen electrode. the photosynthetic o2 evolution was inhibited by am-toxin i in a host-specific manner. the inhibitory activity of several am-toxin analogs against photosynthesis was also evaluated and the findings were correlated with their necrosis-inducing activity. | 2003 | 12723616 |
| characterization of a new pseudomonas aeruginosa strain nj-15 as a potential biocontrol agent. | phylogenetic characterization of soil isolate nj-15, based on sequence homology of a partial 746-bp fragment of 16srdna amplicon, with the ribosomal database sequences (http://www.msu.edu/rdp/cgis/phylip.cgi), validated the strain as pseudomonas aeruginosa. the strain nj-15 produced a substantial amount of indole acetic acid (iaa) in tryptophan-supplemented medium. besides, the strain also exhibited significant production of both the siderophore and hydrogen cyanide (hcn) on chrome azurol s and ... | 2003 | 12732958 |
| fungus research can grow on you. | 2003 | 12775125 | |
| a sensitive two-site enzyme-linked immunosorbent assay for measurement of the major alternaria alternata allergen alt a 1. | alt a 1 is the major allergen in alternaria alternata, one of the most important fungi associated with allergic diseases. mold allergenic extracts show considerable heterogeneity, and thus accurate standardization of these extracts is essential to guarantee their quality. | 2003 | 12775134 |
| cloning and characterization of the mating type (mat) locus from ascochyta rabiei (teleomorph: didymella rabiei) and a mat phylogeny of legume-associated ascochyta spp. | degenerate primers designed to correspond to conserved regions of the high mobility group (hmg) protein encoded by the mat1-2 gene of cochliobolus heterostrophus, cochliobolus sativus, and alternaria alternata were used to amplify the portion of the sequence corresponding to the hmg box motif from ascochyta rabiei (teleomorph: didymella rabiei). a combination of tail and inverse pcr extended the mat1-2 sequence in both directions, then primers designed to mat1-2 flanking dna were used to amplify ... | 2003 | 12781674 |
| mycoflora and ochratoxin-producing strains of aspergillus section nigri in wine grapes in argentina. | the aims of this work were to evaluate the mycoflora and to identify the species of aspergillus with the potential to produce ochratoxin a (oa) from different wine grape varieties from mendoza, argentina. likewise, the capacity to produce oa by aspergillus section nigri was studied. | 2003 | 12859664 |
| liquid chromatography-mass spectrometry and liquid chromatography-tandem mass spectrometry of the alternaria mycotoxins alternariol and alternariol monomethyl ether in fruit juices and beverages. | alternariol (aoh) and alternariol monomethyl ether (ame) are among the main mycotoxins formed in apples and other fruits infected by alternaria alternata. for determination of aoh and ame by lc, apple juice and other fruit beverages were cleaned up on c18 and aminopropyl solid-phase extraction columns. positive and negative ion mass spectra of aoh and ame under electrospray (esi) and atmospheric pressure chemical ionization (apci) conditions were obtained. collision-induced dissociation of the [ ... | 2003 | 12862378 |
| the effect of anti-integrin monoclonal antibodies on antigen-induced pulmonary inflammation in allergic rabbits. | the integrin adhesion molecules are involved in the recruitment and activation of inflammatory cells at sites of inflammation in a variety of diseases. in the present study, we have investigated the effects of blocking monoclonal antibodies (mabs) directed against cd49d (alpha(4) integrin), cd18 (beta(2) integrin) and the alpha sub-units of beta(2) integrin cd11a (lfa-1 integrin) and cd11b (mac-1 integrin), on antigen (ag)-induced acute bronchoconstriction and cellular recruitment in allergic ra ... | 2003 | 12877819 |
| hla class ii genotypic frequencies in atopic asthma: association of drb1*01-dqb1*0501 genotype with artemisia vulgaris allergic asthma. | different human leukocyte antigen (hla) class ii alleles have been associated with the development of atopic asthma. to determine whether hla class ii alleles are associated with atopic asthma in a population from southeast spain (murcia region), 213 atopic asthmatic patients and 150 controls were selected for hla typing. significant association of the drb1*01 and dqb1*0501 alleles was found in artemisia vulgaris allergic patients (p(c) = 0.00052 and p(c) = 0.00023, respectively). no significant ... | 2003 | 12878360 |
| immunotherapy in fungal allergy. | for decades airborne fungal spores have been implicated as causative factors in respiratory allergy. exposure to high atmospheric spore counts and sensitization to specific fungal allergens have been associated with severe asthma, mainly in young adults. although the prevalence of sensitization to commercial fungal extracts is approximately 3% in epidemiologic studies, in selected patients, particularly with asthma, the sensitization rate might increase to 30%. of the estimated number of more th ... | 2003 | 12906784 |
| identity and conservation of mating type genes in geographically diverse isolates of phaeosphaeria nodorum. | mating type idiomorphs (mat1-1 and mat1-2) were identified from the heterothallic loculoascomycete phaeosphaeria nodorum (wheat biotype) using dna from a pair of isolates from poland and georgia, usa that are known to mate. mat predicted proteins of p. nodorum are similar in sequence and in phylogenetic relationship to those described for other loculoascomycetes such as cochliobolus spp., alternaria alternata, and didymella zeae-maydis. the organization of the mat locus of the p. nodorum differs ... | 2003 | 12948511 |
| purification and some important characters of extracellular inulinase of alternaria alternata (fr.) keissler. | protein precipitate of cell-free dialysate of extracellular inulinase (2,1-beta-fructan fructanohydrolase, ec 3.2.1.7) of a. alternata was maximally obtained by methanol. such protein was fractionated by using 2-step column chromatography on sephadex g150 and deae-cellulose. the partially purified enzyme had activity of 81 x 10(3) u/mg protein, with a yield of 69% of the original activity and the fold of purification was 62. optimum temperature and ph for the activity of the purified enzyme were ... | 2002 | 12974403 |
| studies in the biochemistry of microorganisms. 90. alternariol and alternariol monomethyl ether, metabolic products of alternaria tenuis. | 1953 | 13105649 | |
| studies in allergenicity of extracts prepared from sporulating and nonsporulating alternaria tenuis. | 1957 | 13405692 | |
| studies in the biochemistry of micro-organisms. 103. metabolites of alternaria tenuis auct; culture filtrate products. | 1957 | 13479395 | |
| [effect of sources of carbon on morphological and parasitic properties of the causative agent of clover sprouts diseases alternaria tenuis ness]. | 1957 | 13483133 | |
| studies in the biochemistry of micro-organisms. 106. metabolites of alternaria tenuis auct.: the structure of tenuazonic acid. | 1959 | 13662306 | |
| on a rare disease due to alternaria tenuis nees (alternariasis). | 1960 | 13730495 | |
| studies in the biosynthesis of fungal metabolites. 4. alternariol monomethyl ether and its relation to other phenolic metabolites of alternaria tenuis. | 1961 | 13776530 | |
| [studies on the occurrence of sectors in fungal colonies, especially in alternaria tenuis]. | 1955 | 14388714 | |
| facile one pot microwave induced solvent-free synthesis and antifungal, antitubercular screening of spiro [1,5]-benzothiazepin-2,3'[3'h]indol-2[1'h]-ones. | microwave activation coupled with dry media technique as a green chemistry procedure has been applied to synthesis of a series of some new title compounds. they have been obtained by the reaction of in situ synthesized 1,3-dihydro-3-[2-(phenyl/4-fluorophenyl)-2-oxoethylidene)-indol-2(1h)-one (4a, b) with substituted aminobenzenethiols (5a-d). the key intermediates 4a, b were also prepared in one step by this improved technique by reacting isatin and substituted acetophenones (2a, b). the results ... | 2003 | 14519917 |
| ige-mediated allergy to fungal allergens in finland with special reference to alternaria alternata and cladosporium herbarum. | alternaria alternata and cladosporium herbarum are common fungi in outdoor environments, but their clinical significance has not been elucidated in finland. | 2003 | 14533661 |
| characterization of a hydroperoxide lyase gene and effect of c6-volatiles on expression of genes of the oxylipin metabolism in citrus. | a number of c6-volatile products of the lipoxygenase (lox) pathway was examined for their antifungal activity and a potential role as a signal molecule in citrus. trans-2-hexenal induced the rough lemon lipoxygenase gene (rlemlox), hydroperoxide lyase gene (rlemhpl) and aos gene, but hexanal, and hexanol suppressed them. cis-3-hexenol and trans-2-hexenol increased expression of the aos gene but not rlemlox and rlemhpl. transcripts of the rlemhpl and aos gene were detected constitutively in leave ... | 2003 | 14610891 |
| the development of alternaria alternata is prevented by chitinases and beta-1,3-glucanases from citrus limon seedlings. | in addition to phytoalexin synthesis, the defense response of intact citrus limon seedlings against alternaria alternata involves both constitutive and induced enzyme activities such as chitinases (ch) and beta-1,3-glucanases (glu). a alternata conidial germination was prevented by protein extracts from inoculated lemon seedlings, but also by extracts from mock-inoculated specimens. on the other hand, degradation of mycelia was accomplished only by protein extracts from inoculated seedlings. the ... | 2003 | 14631873 |
| expression profiles of arabidopsis phospholipase a iia gene in response to biotic and abiotic stresses. | we examined the transcripts that showed changes among the ca.7,000 arabidopsis full-length cdnas under biotic and abiotic stresses. expression of arabidopsis phospholipase a iia (atpla iia) gene was induced by various treatments such as pathogen inoculation (alternaria alternata, alternaria brassicicola and colletotrichum higginsianum), cold, high-salinity, abscisic acid, salicylic acid, methyl jasmonate, ethephon, paraquat, rose bengal, uv-c and cuso(4)-treatments. the regulation of atpla iia g ... | 2003 | 14634163 |
| a case of indirect exposure to cat at school. | indirect exposure to cat allergens may exacerbate asthma in sensitized subjects. we report a case of a 13-year-old girl referred to our unit for cough, dyspnea, wheezing, chest tightness, nasal itching and obstruction during the past six months, with improvement during summer holidays. skin prick tests were positive for cat and alternaria alternata. she had no cats at home. spirometry was normal and methacoline bronchial challenge was negative. pef monitoring showed a mean value of 80% of the pr ... | 2003 | 14635509 |
| dicarboximide resistance in field isolates of alternaria alternata is mediated by a mutation in a two-component histidine kinase gene. | isolates of alternaria alternata collected from a field site which had previously been treated with the dicarboximide fungicide iprodione were found to demonstrate a high level of resistance to iprodione and the phenylpyrrole fungicide, fludioxonil in plate assays. in order to determine the genetic basis for this fungicide resistance a partial length clone of a two-component histidine kinase (hk) was isolated from genomic dna of a fungicide-sensitive a. alternata isolate using degenerate primers ... | 2004 | 14643263 |
| [positive skin test and age] | objective: to evaluate positive responses to skin tests for immediate hypersensitivity to allergens in children with asthma and rhinitis at different ages. method: we observed positive skin test reactivity in prick tests using fifteen allergens of same origin (total dust and dermatophagoides sp.; dermatophagoides pteronyssinus; dermatophagoides farinae; blomia tropicalis; penicillium sp; alternaria alternata; cladosporium herbarium; aspergillus fumigatus; bermuda grass; forage grass; dog and cat ... | 2001 | 14647601 |
| concentration and species diversity of airborne fungi near busy streets in lithuanian urban areas. | the investigations on air pollution in industrial cities of lithuania: vilnius, alytus, kaunas, marijampole and elektrenai were aimed at detecting the presence of fungi and aerosol particles during different seasons of the year. sampling of fungal spores was carried out at 20 sampling sites. active air sampling was performed simultaneously with the use of passive sedimentation plates. data on the spread of various micromycete species in the air of cities contaminated with various pollutants are ... | 2003 | 14677918 |
| exposure to bioaerosols in a municipal sewage treatment plant. | microbiological air sampling was performed in a medium-size sewage treatment plant processing municipal wastewater from a city located in eastern poland. air samples for determination of the concentrations of viable mesophilic bacteria, gram-negative bacteria, thermophilic actinomycetes, fungi and endotoxin were collected at 12 sites associated with various phases of sewage treatment process. the concentrations of total mesophilic bacteria (both gram-positive and gram-negative) were within a ran ... | 2003 | 14677919 |
| [clinical characteristics of the fatal and near-fatal asthma in alternaria alternata sensitized patients]. | some studies have pointed to alternaria alternata as a trigger of fatal and near-fatal asthma (nfa) crises. the objective of this study was to determine the clinical characteristics of fatal and nfa crisis in patients sensitized to alternaria. | 2003 | 14678692 |
| isolation of alternaria alternata from an emollient cream: implications for public health. | a specimen of emollient cream, which was observed to be contaminated peripherally with a filamentous fungus was examined for the presence of fungi and the resulting fungal colonies were examined phenotypically and genotypically. subsequent dna extraction and pcr amplification of the large internal transcribed spacer region [its1-5.8s-its2] yielded an amplicon of 512 bp. sequence analysis identified this as alternaria alternata at the 100% homology level with all 512/512 bases called. this organi ... | 2003 | 14682451 |
| effect of the k+(atp) channel opener, kco912, on baseline and allergen induced airway hyperresponsiveness in allergic rabbits. | the effect of the adenosine triphosphate sensitive k+ (k(atp)) channel opener (3s,4r)-3,4-dihydro-3-hydroxy-2,2-dimethyl-4-(2-oxo-1-piperidinyl)-n-phenyl-1-benzopyran-6-sulphonamide (kco912) on airway hyperresponsiveness induced using either a combination of allergen immunization (i.p.) followed by aerosol allergen challenge or immunization alone was investigated. rabbits were immunized with alternaria tenuis for the first 3 months of life. airway responsiveness to histamine was measured 24 h be ... | 2004 | 14744622 |
| epoxide hydrolase: a mrna induced by the fungal pathogen alternaria alternata on rough lemon (citrus jambhiri lush). | an expression profile of genes induced by non-pathogenic alternaria alternata on rough lemon leaves was obtained by sequencing 500 subtractive pcr clones generated from mrna of leaves inoculated with the fungus after subtraction with that of non-inoculated leaves. about 6% of the cdna sequences had homology to known putative defense-related genes including epoxide hydrolase. a full-length cdna (951 bp) from rough lemon that encoded epoxy hydrolase was isolated by random amplification of cdna end ... | 2003 | 14756316 |
| [quick method for determination of lysozyme activity]. | 1950 | 14773542 | |
| effect of beta-indol-3-acetic acid, phenoxy acetic acid and beta-naphthoxy acetic acid on growth of alternaria tenuis. | 1950 | 14778308 | |
| purification and characterization of a major cross-reactive allergen from epicoccum purpurascens. | epicoccum purpurascens (formerly nigrum) (ep), is a ubiquitous saprophytic mould found both indoors and outdoors. several studies have reported sensitization to ep in 5-7% of different populations worldwide. the diagnosis of mould allergy requires a standardized fungal extract that contains all its important allergenic proteins. the crude allergen extract from ep was standardized earlier, however none of its allergens have been purified. | 2004 | 14976389 |
| two plant alkaloids isolated from corydalis longipes as potential antifungal agents. | the alkaloids n-methylhydrasteine hydroxylactam and 1-methoxyberberine chloride were isolated from corydalis longipes. both alkaloids showed high efficacy individually (in concentration of 50-150 ppm) and also in a 1:1 mixture against spore germination of some fungi, viz. alternaria alternata, a. brassicae, curvularia maculans, curvularia sp., colletotrichum gloeosporioides, colletotrichum sp., helminthosporium speciferum, h. pennisetti, helminthosporium sp., and ustilago cynodontis. the antifun ... | 2003 | 14976716 |
| phytotoxic and antimicrobial constituents of bacopa monnieri and holmskioldia sanguinea. | the phytochemicals betulinic acid (1), wogonin (2) and oroxindin (3) isolated from the aerial parts of bacopa monnieri and holmskioldia sanguinea showed significant antifungal activity against the two fungi alternaria alternata and fusarium fusiformis. inhibition of root growth germination of wheat seeds was observed for all three compounds which showed 100% inhibition at 10 micro g/ml. compounds (1) and (2) showed potent inhibition of alternaria alternata compared with oroxindin at a concentrat ... | 2004 | 15022161 |
| dissection of the host range of the fungal plant pathogen alternaria alternata by modification of secondary metabolism. | the filamentous fungus alternaria alternata contains seven pathogenic variants (pathotypes), which produce different host-specific toxins and cause diseases on different plants. the strawberry pathotype produces host-specific af-toxin and causes alternaria black spot of strawberry. this pathotype is also pathogenic to japanese pear cultivars susceptible to the japanese pear pathotype that produces ak-toxin. the strawberry pathotype produces two related molecular species, af-toxins i and ii: toxi ... | 2004 | 15066029 |
| cutaneous alternariosis in an immunocompetent patient: analysis of the internal transcribed spacer region of rdna and brm2 of isolated alternaria alternata. | 2004 | 15099381 | |
| effect of static magnetic field on growth and sporulation of some plant pathogenic fungi. | growth and sporulation of phytopathogenic microscopic fungi were studied under a static magnetic field. the applied flux densities were 0.1, 0.5, and 1 mt. the magnetic field decreased the growth of colonies by 10% using this flux density region. at 0.1 mt flux density, the deviations are significant, p =.001, while in other cases the deviations generally are not significant. at the same time, the number of the developed conidia of alternaria alternata and curvularia inaequalis increased by 68-1 ... | 2004 | 15114642 |
| survival of spores of rhizopus stolonifer, aspergillus niger, botrytis cinerea and alternaria alternata after exposure to ethanol solutions at various temperatures. | to quantify and model the toxicity of brief exposures of spores of rhizopus stolonifer, aspergillus niger, botrytis cinerea and alternaria alternata to heated, aqueous ethanol solutions. these fungi are common postharvest decay pathogens of fresh grapes and other produce. sanitation of produce reduces postharvest losses caused by these and other pathogens. | 2004 | 15139929 |
| an extensive microarray analysis of aal-toxin-induced cell death in arabidopsis thaliana brings new insights into the complexity of programmed cell death in plants. | a t-dna knockout of the arabidopsis homologue of the tomato disease resistance gene asc was obtained. the asc gene renders plants sensitive to programmed cell death (pcd) triggered by the fungal aal toxin. to obtain more insights into the nature of aal-toxin-induced cell death and to identify genes of potential importance for pcd, we carried out transcription profiling of aal-toxin-induced cell death in this knockout with an oligonucleotide array representing 21,500 arabidopsis genes. genes resp ... | 2004 | 15141304 |
| airborne viable, non-viable, and allergenic fungi in a rural agricultural area of india: a 2-year study at five outdoor sampling stations. | the information on airborne allergenic fungal flora in rural agricultural areas is largely lacking. adequate information is not available to the bioaerosol researchers regarding the choice of single versus multiple sampling stations for the monitoring of both viable and non-viable airborne fungi. there is no long-term study estimating the ratios of viable and non-viable fungi in the air and earlier studies did not focus on the fractions of airborne allergenic fungi with respect to the total airb ... | 2004 | 15142771 |
| sodium bicarbonate enhances biocontrol efficacy of yeasts on fungal spoilage of pears. | the addition of 2% (w/v) sodium bicarbonate (sbc) in the suspensions of antagonistic yeast cryptococcus laurentii or trichosporon pullulans significantly limited spore germination and germ tube elongation of penicillium expansum and alternaria alternata in potato dextrose broth (pdb) medium. biocontrol activity of c. laurentii or t. pullulans against postharvest decay caused by p. expansum and a. alternata in pear fruits was significantly increased when c. laurentii or t. pullulans combined with ... | 2004 | 15163586 |
| frequency and diversity of fungi colonizing tissues of upland cotton. | a study of the mycoflora of upland cotton in alabama was conducted throughout the 2000 and 2001 growing seasons. plants were sampled at seedling, first bloom, full bloom, and maturity stages of development. thirty-seven genera representing 58 species of fungi were isolated, including 9 species of fusarium. fusarium oxysporum, f. solani, and f. equiseti were the most common members of this genus occurring at all four sampling stages in both years. eight species accounted for 67% of the total fung ... | 2004 | 15180159 |
| alternaria spores at different heights from the ground. | alternaria tenuis (alt) is one of the main allergens in pediatric age. in temperate climates, airborne alt spores are detectable from may to november with peaks in late summer and autumn. sensitized children display symptoms even in the absence of airborne alt spores. alt spore concentration, as well as pollen, is usually detected by fixed devices located on the roof of a building at a height of 10-20 m. the aim of the current study is to find out whether ground-level (50 cm) alt spore concentra ... | 2004 | 15180762 |
| specific 12 beta-hydroxylation of cinobufagin by filamentous fungi. | biotransformation of natural products has great potential for producing new drugs and could provide in vitro models of mammalian metabolism. microbial transformation of the cytotoxic steroid cinobufagin was investigated. cinobufagin could be specifically hydroxylated at the 12 beta-position by the fungus alternaria alternata. six products from a scaled-up fermentation were obtained by silica gel column chromatography and reversed-phase liquid chromatography and were identified as 12 beta-hydroxy ... | 2004 | 15184152 |
| cell-lytic activity of tobacco by-2 induced by a fungal elicitor from alternaria alternata attributed to the expression of a class i beta-1,3-glucanase gene. | stress-induced cell-lytic activity was found in tobacco by-2 cells treated with various stresses. among 14 stresses, an elicitor fraction isolated from alternaria alternata showed the highest inducing activity. cell-lytic activity increased for 72 h even in the control sample, treated with distilled water, and several isozymes of beta-1,3-glucanases and chitinases were found to be involved in it. in contrast, cell-lytic activity in by-2 cells treated with a fungal elicitor reached a higher level ... | 2004 | 15215590 |
| exposure of hop growers to bioaerosols. | air sampling was performed during picking and sorting of hop (humulus lupulus) cones on 19 hop farms located in eastern poland. the concentration and composition of airborne microflora and the concentration of airborne dust and endotoxin were determined. additionally, 7 samples of settled hop dust were collected and examined for the presence of microorganisms and endotoxin. total concentrations of airborne microorganisms were within a range of 2.08-129.58 x 10(3) cfu/m(3). gram-positive bacteria ... | 2004 | 15236510 |
| field study on changes in viability of airborne fungal propagules exposed to uv radiation. | the responses of airborne fungi to uv-b under natural conditions were investigated at the coastal station in preila, lithuania. results of this investigation demonstrated that solar radiation has a marked lethal effect on outdoor airborne fungi. sensitivity to solar radiation was the highest for the fungal propagules collected late in the evening (relative recovery 6.2%) and early morning (25.3%). the lowest sensitivity to solar radiation was observed for fungal propagules collected at midday (5 ... | 2004 | 15269921 |
| cutaneous infection caused by alternaria in patients receiving tacrolimus. | tacrolimus, an immunosuppressant used in organ transplant surgery, is inhibitory to some medically important fungi but also may obstruct azole monotherapy in the immunocompromised patient. we observed a case of cutaneous phaeohyphomycosis caused by alternaria alternata in a liver transplant recipient who had been under tacrolimus immunosuppression for 6 months post-transplantation. at the onset of the infection, the patient presented with an increased whole-blood tacrolimus level. after a simple ... | 2004 | 15283243 |
| inhibitory effect of pollen and propolis extracts. | bee pollen and propolis were collected from apis mellifera colonies in five regions of turkey. the antifungal properties of methanol extracts of pollen and propolis (2% and 5% concentrations) were determined on alternaria alternata and fusarium oxysporium f. sp. melonis. the least active concentration towards the tested fungi was 2% concentration of both extracts. the inhibitory effect of all propolis extracts on growth of f. oxysporium and a. alternata were generally higher when compared with p ... | 2004 | 15285109 |
| allergens in japanese patients with allergic conjunctivitis in autumn. | the purpose of the current study is to evaluate the relation between various specific class e immunoglobulins (ige) in the serum and allergic conjunctivitis in autumn. | 2005 | 15389269 |
| a comparison of the effects of ocular preservatives on mammalian and microbial atp and glutathione levels. | the aim of this study was to investigate the mechanism of action of the preservative sodium chlorite (naclo2), and the relationship with intracellular glutathione depletion. a detailed comparison of the dose responses of two cultured ocular epithelial cell types and four species of microorganism was carried out, and comparisons were also made with the quaternary ammonium compound benzalkonium chloride (bak), and the oxidant hydrogen peroxide (h2o2). the viability of mammalian and microbial cells ... | 2004 | 15453639 |
| mycoflora and potential for mycotoxin production of freshly harvested black bean from the argentinean main production area. | a mycological survey was carried out, for the first time, on black bean samples from the northwestern argentinean province of salta in the 1999 harvest season. ten varieties of black beans were evaluated at three locations. species of the genus alternaria were the most prevalent component of the black bean mycoflora. species of fusarium, sclerotinia, rhizoctonia and acremonium were also recorded. the predominant species of the genera isolated were alternaria alternata, sclerotinia sclerotiorum, ... | 2004 | 15487328 |
| a review of alternaria alternata sensitivity. | sensitivity to fungi is a major risk factor for the development of asthma. however, the prevalence of fungal sensitivity in asthma is not completely understood. nonetheless upward of 80% of asthmatic patients may be sensitized to one or more fungi. fungal exposure occurs primarily from outdoors sources, but can occur in the indoor environment as well. assessment of fungal exposure requires a multifaceted approach including measurement of airborne spores and culture techniques to identify the rel ... | 2001 | 15487907 |
| accumulation of hdmboa-glc is induced by biotic stresses prior to the release of mboa in maize leaves. | the effects of biotic stresses on the contents of benzoxazinones (bxs) were investigated in maize leaves. when the causal agent of southern corn leaf blight, bipolaris maydis, was inoculated on the third leaf, the amount of 2-hydroxy-4,7-dimethoxy-1,4-benzoxazin-3-one glucoside (hdmboa-glc) increased, reaching a maximum level 48 h after inoculation. the inoculation of weakly pathogenic curvularia lunata and non-pathogenic alternaria alternata also resulted in accumulation of hdmboa-glc, and filt ... | 2004 | 15504434 |
| pathologic quiz case: a 56-year-old woman with anterior nasal pain and intermittent epistaxis. alternaria alternate infection of the nasal sinus. | 2004 | 15578896 | |
| metabiotic associations of molds and salmonella poona on intact and wounded cantaloupe rind. | salmonella poona, a serotype rarely implicated in human infections, has recently caused several cantaloupe-associated outbreaks of salmonellosis. metabiotic associations of molds and foodborne pathogens on produce have been reported. we tested proteolytic activity and measured changes in the ph of cantaloupe rind caused by growth of alternaria alternata, cladosporium cladosporioides, epicoccum nigrum, geotrichum candidum, and penicillium expansum. survival and growth characteristics of salmonell ... | 2005 | 15582743 |