Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| enzyme-functionalized polymer brush films on the inner wall of silicon-glass microreactors with tunable biocatalytic activity. | the lipase from candida rugosa was immobilized to a poly(methacrylic acid) polymer brush layer, grown on the inner wall of silicon-glass microreactors. the hydrolysis of 4-nitrophenyl acetate was used as a model reaction to study the activity of this biocatalytic system. the amount of bound lipase could be tuned by changing the polymerization time of the brush formation. the michaelis-menten constants and v(max) values, determined for immobilized and free lipase, are similar, demonstrating that ... | 2010 | 20941436 |
| recombinant candida rugosa lipase 2 from pichia pastoris: immobilization and use as biocatalyst in a stereoselective reaction. | the characterization of the recombinant candida rugosa lip2 (r-lip2) isoenzyme obtained from fed-batch cultures of pichia pastoris under paox promoter was carried out, determining the optimal ph and temperature as well as their catalytic performance in both hydrolysis and synthesis reactions comparing with purified native lip2 (n-lip2) previously determined. the substrate specificity of r-lip2 in hydrolysis reactions was determined with a series of triacylglycerols and p-nitrophenyl esters of va ... | 2010 | 20945483 |
| a highly stable yarrowia lipolytica lipase formulation for the treatment of pancreatic exocrine insufficiency. | yarrowia lipolytica lipase has been assumed to be a good candidate for the treatment of fat malabsorption in patients with pancreatic insufficiency. nevertheless, no systematic studies on its stability under physiological conditions pertaining to the human gi (gastrointestinal) tract have been published. stability of various y. lipolytica lipase powder formulations at various physiological ph values as well as the effect of digestive proteases and bile salts on enzyme activity were investigated. ... | 2010 | 20958263 |
| in vitro activity of fluconazole and voriconazole against clinical isolates of candida spp. by e-test method. | the in vitro susceptibility of clinical candida isolates towards fluconazole and voriconazole was determined using the e-test method. a total of 41 clinical isolates recovered from patients since 2004 until 2009 from two local hospitals in kuala lumpur, malaysia were used. these comprised candida tropicalis, candida albicans, candida krusei, candida parapsilosis, candida rugosa, candida dubliniensis and candida glabrata. strains from american type culture collection were used as quality control. ... | 2010 | 20962716 |
| enzymatic hydrolysis of polylactic acid fiber. | this study investigated the optimization of the enzymatic processing conditions for polylactic acid (pla) fibers using enzymes consisting of lipases originating from different sources. the hydrolytic activity was evaluated taking into consideration the ph, temperature, enzyme concentration, and treatment time. the structural change of the pla fibers was measured in the optimal treatment conditions. pla fiber hydrolysis by lipases was maximized for lipase from aspergillus niger at 40 °c for 60 mi ... | 2010 | 21038086 |
| biochemical properties of free and immobilized candida rugosa lipase onto al(2)o(3): a comparative study. | abstract: lipase from candida rugosa (crl) was immobilized by physical adsorption onto al(2)o(3). the immobilization yield was more than 95% during 30 min. the properties of free and immobilized enzyme were also searched and compared. the optimum ph was shifted from 7 to 8.5 by immobilization of the enzyme. the maximum activity of the free and the immobilized enzymes occured at 35 and 40°c, respectively. | 2010 | 21117873 |
| epidemiology of candidaemia in critically ill trauma patients: experiences of a level i trauma centre in north india. | there is a need to understand the epidemiology and risk factors associated with candidaemia in critically ill trauma patients. the rise in incidence of non-albicans candidaemia and the emergence of antifungal resistance have made such a study necessary. a prospective laboratory-based surveillance study was performed over a period of 21 months (april 2008-december 2009) at a level i trauma centre in new delhi, india. all blood culture samples positive for candida were processed for microbial iden ... | 2010 | 21127153 |
| intracellular characterization of aerobic glucose metabolism in seven yeast species by 13c flux analysis and metabolomics. | key distinguishing characteristics of yeast glucose metabolism are the relative proportions of fermentation and respiration. crabtree-positive yeast species exhibit a respirofermentative metabolism, whereas aerobic species respire fully without secretion of fermentation byproducts. physiological data suggest a gradual transition in different species between these two states. here, we investigate whether this gradual transition also occurs at the intracellular level by quantifying the intracellul ... | 2011 | 21205161 |
| candida rugosa: a new fungal pathogen emerging, but from where? | 2011 | 21212147 | |
| improvement of catalytic activity of lipase from candida rugosa via sol-gel encapsulation in the presence of calix(aza)crown. | lipase from candida rugosa (crl) was encapsulated within a chemically inert sol-gel support in the presence of calix(aza)crowns as the new additives. the catalytic activity of the encapsulated lipases was evaluated both in the hydrolysis of p-nitrophenyl palmitate (p-npp) and the enantioselective hydrolysis of racemic naproxen methyl ester. it has been observed that the percent activity yields of the calix(aza)crown based encapsulated lipases were higher than that of the free lipase. improved en ... | 2011 | 21256747 |
| conformational flexibility of lipase lip1 from candida rugosa studied by electronic spectroscopies and thermodynamic approaches. | we have used second-order orthogonal designs to obtain empirical models that describe the combined effect of ph and temperature on the secondary structure of a lipase (lip1) from candida rusosa. the equations that describe lipase conformational flexibility were derivated from the enzyme alpha helix fraction obtained from the experimental matrix. the thermal unfolding of lipase at different ph values was followed by measuring the circular dichroism signal as a function of temperature over a tempe ... | 2011 | 21318273 |
| mechanism of acetaldehyde-induced deactivation of microbial lipases. | microbial lipases represent the most important class of biocatalysts used for a wealth of applications in organic synthesis. an often applied reaction is the lipase-catalyzed transesterification of vinyl esters and alcohols resulting in the formation of acetaldehyde which is known to deactivate microbial lipases, presumably by structural changes caused by initial schiff-base formation at solvent accessible lysine residues. previous studies showed that several lipases were sensitive toward acetal ... | 2011 | 21342514 |
| immobilization of lipase from candida rugosa on sepabeads(®): the effect of lipase oxidation by periodates. | the objective of this paper was the investigation of a suitable sepabeads(®) support and method for immobilization of lipase from candida rugosa. three different supports were used, two with amino groups, (sepabeads(®) ec-ea and sepabeads(®) ec-ha), differing in spacer length (two and six carbons, respectively) and one with epoxy group (sepabeads(®) ec-ep). lipase immobilization was carried out by two conventional methods (via epoxy groups and via glutaraldehyde), and with periodate method for m ... | 2011 | 21347667 |
| enantioselectivity of candida rugosa lipases (lip1, lip3, and lip4) towards 2-bromo phenylacetic acid octyl esters controlled by a single amino acid. | enantiomer discrimination by enzymes is a very accurate mechanism, which often involves few amino acids located at the active site. lipase isoforms from candida rugosa are very good enzymatic models to study this phenomenon as they display high sequence homology (>80%) and their enantioselectivity is often pointed out. in the present work, we investigated three lipases from c. rugosa (lip1, lip3, and lip4, respectively) towards the resolution of 2-bromo-arylacetic acid esters, an important class ... | 2011 | 21391204 |
| selective biocatalytic aminolysis of (±)-epichlorohydrin: synthesis and icam-1 inhibitory activity of (s)-(+)-3-arylamino-1-chloropropan-2-ols. | regio- and enantioselective synthesis of (s)-(+)-3-arylamino-1-chloropropan-2-ols has been achieved by the epoxide ring opening of (±)-epichlorohydrin with different aromatic amines in the presence of candida rugosa lipase. activities of seven model (s)-(+)-3-arylamino-1-chloropropan-2-ols, out of 10 compounds synthesized, have been evaluated for the inhibition of tumor necrosis factor-a tnf-a) induced expression of intercellular adhesion molecule-1 (icam-1), which is one of the factors responsi ... | 2011 | 21419631 |
| chemoenzymatic synthesis with lipase catalyzed resolution and evaluation of antitumor activity of (r/s)-2-[2-hydroxy-3-(4-phenylpiperazin-1-yl)propyl]-1h-pyrrolo[3,4-b]quinolin-3(2h)-one. | synthesis, characterization, resolution and evaluation of novel (r/s)-2-[2-hydroxy-3-(4-phenylpiperazin-1-yl)propyl]-1h-pyrrolo[3,4-b]quinolin-3(2h)-one derivatives are described. enantiomerically pure compounds were isolated in good to excellent yield with high enantiomeric excess under mild reaction conditions by using candida antarctica b (cal-b) and candida rugosa (crl) lipases. newly synthesized and resolved compounds were screened for their antitumor activity against cancer cells such as h ... | 2011 | 21440337 |
| c-terminal region of candida rugosa lipases affects enzyme activity and interfacial activation. | candida rugosa contains several lipase (crls) genes, and crls show diverse enzyme activity despite being highly homologous across their entire protein family. previous studies found that lip4 has a high esterase activity and a low lipolytic activity and lacks interfacial activation. to investigate whether the c-terminal region of the crls mediates enzymatic activity, chimeras were generated in which the c-terminus of lip4 from either residue 374, 396, 417, or 444 to residue 534 was swapped with ... | 2011 | 21504227 |
| improvement of catalytic properties of candida rugosa lipase by sol-gel encapsulation in the presence of magnetic calix[4]arene nanoparticles. | candida rugosa lipase (crl) was encapsulated within a chemically inert sol-gel support prepared by polycondensation with tetraethoxysilane (teos) and octyltriethoxysilane (otes) in the presence of n-methylglucamine based calix[4]arene magnetic nanoparticles. the results indicate that the magnetic calix[4]arene based encapsulated lipase particularly has shown high conversion and enantioselectivity. it has also been noticed that the magnetic calix[4]arene based encapsulated lipase has excellent en ... | 2011 | 21509361 |
| engineering the expression and biochemical characteristics of recombinant candida rugosa lip2 lipase by removing the additional n-terminal peptide and regional codon optimization. | candida rugosa lipase (crl), an important industrial enzyme, has been established, containing several different isoforms which were encoded by the high-identity lip gene family (lip1 to lip7). in this study, we compared the expression and biochemical characterization with three different engineered lip2 constructions in the yeast pichia pastoris. our results showed that lip2 (lip2) has an overall improvement of 50% higher production yield (1.446 u/ml) relative to that of nflip2 (0.964 u/ml) at 7 ... | 2011 | 21561168 |
| lipase-catalyzed synthesis of hydrophobically modified dextrans: activity and regioselectivity of lipase from candida rugosa. | vinyl decanoate-modified dextran macromolecules (dext40-vd) were synthesized in dimethyl sulfoxide at 50°c using lipase ay from candida rugosa for catalyzing transesterification between polysaccharide and vinyl fatty esters. the extent of dextran modification (quantified by the molar ratio of attached alkyl tails to sugar repeat units) with native-, ph-adjusted-, 18-crown-6 ether pretreated ph-adjusted-, and stepwise addition of pretreated lipase ay yielded <3%, 49%, 64% and 96% modified dextran ... | 2011 | 21602099 |
| cationic polyelectrolytes-lipases complexes formation as tool for recovery of these enzymes from their natural sources. | in the present paper the formation of complexes between positively charge polyelectrolyte (polyethyleneimine and chitosan) and candida rugosa lipase from a crude extract and porcine lipase from pancreas commercial homogenate preparations were analyzed. the solubility of lipases-cationic polyelectrolytes formation was dependent on: polyelectrolyte densities electrical charge, polyelectrolyte and enzyme concentrations and salts present in the solution. the lipase was recovered from the non-soluble ... | 2011 | 21640134 |
| enhancement of activity and selectivity of candida rugosa lipase and candida antarctica lipase a by bioimprinting and/or immobilization for application in the selective ethanolysis of fish oil. | candida rugosa lipase (crl) and candida antarctica lipase a (cala) with improved activity and selectivity were prepared for use in organic solvent media. crl bioimprinted with fatty acids exhibited eightfold enhanced transesterification activity in hexane. combination of bioimprinting and coating with lecithin or with immobilization did not improve the activity further. cala was immobilized with and without bioimprinting, none of which improved the activity. all modified lipases were tested for ... | 2011 | 21695486 |
| phenotypic and genotypic characterisation of two closely related subgroups of candida rugosa in the clinical specimens. | in this study, six clinical isolates (two from blood, two from urine, and one each from bronchoalveolar lavage and vaginal swab) were identified as candida rugosa based on carbohydrate assimilation profiles by api 20c aux and id32 kits. sequence analysis of the d1/d2 domain of the yeasts differentiated the isolates into two subgroups, a and b (three isolates per subgroup) which are closely related (99.1-99.6% similarity) to c. rugosa atcc 10571 strain. comparing to c. rugosa type strain, the its ... | 2011 | 21700741 |
| cloning and transcription analysis of the candida rugosa propionyl-coa dehydrogenase gene and its expression in pichia pastoris. | the propionyl-coa dehydrogenase (pacd) gene was cloned from candida rugosa by the cdna race technique. the full cdna of the pacd gene has a length of 1408 bp, which contains a complete open reading frame (orf) of 1329 bp, coding for 442 amino acids. the cdna of pacd was cloned into the expression plasmid ppic9k and transformed into pichia pastoris gs115. the recombinant protein was purified by ni-nta affinity chromatography, and its size was observed to be approximately 49 kda as estimated by sd ... | 2011 | 21780146 |
| characterization of codon-optimized recombinant candida rugosa lipase 5 (lip5). | recombinant candida rugosa lipase 5 (lip5) has been functionally expressed along with other isoforms in our laboratory. however, the characterization and codon optimization of lip5 have not been done. in this work, we characterized, codon-optimized and compared lip5 with commercial lipase. lip5 activity on hydrolysis of p-nitrophenyl (p-np) butyrate was optimal at 55 °c as compared with 37 °c of the commercial lipase. several assays were also performed to determine the substrate specificity of l ... | 2011 | 21854055 |
| short communication: epidemiology and genotyping of candida rugosa strains responsible for persistent intramammary infections in dairy cows. | the present study was undertaken during an outbreak of clinical and subclinical mastitis in 14 dairy cows caused by candida rugosa, in which high somatic cell counts were seen and cases did not respond to antibiotic treatment. intramammary infection cured spontaneously in 10 cows, whereas 4 cows were culled as a result of persistent infections. repeated sampling of these cows and biomolecular analysis of the isolates showed that the infections were caused by the same genotype, even over a period ... | 2011 | 21854930 |
| immobilization of candida rugosa lipase on poly(3-hydroxybutyrate-co-hydroxyvalerate): a new eco-friendly support. | the overall objective of this study is to evaluate the morphological [scanning electron microscopy (sem)], physicochemical [differential scanning calorimetry (dsc), thermogravimetric analysis (tga), chemical composition analysis, fourier-transform infrared spectroscopy (ftir), nuclear magnetic resonance (nmr)], and biochemical properties of candida rugosa lipase (crl) immobilized on a natural biopolymer poly(3-hydroxybutyrate-co-hydroxyvalerate) (phbv) in aqueous solution. crl was immobilized by ... | 2011 | 21870100 |
| Assessment of the Morphological, Biochemical, and Kinetic Properties for Candida rugosa Lipase Immobilized on Hydrous Niobium Oxide to Be Used in the Biodiesel Synthesis. | Lipase from Candida rugosa (CRL) was immobilized by covalent attachment on hydrous niobium oxide. The matrix could effectively be attached to the enzyme with high retention of activity and prevent its leakage. Following immobilization, CRL exhibited improved storage stability and performed better at higher incubation temperatures. In addition, the enzyme retained most of its catalytic efficiency after successive operational cycles. The immobilized derivative was also fully characterized with res ... | 2011 | 21876790 |
| enantioselective enzymatic hydrolysis of racemic drugs by encapsulation in sol-gel magnetic sporopollenin. | candida rugosa lipase was encapsulated within a sol-gel procedure and improved considerably by fluoride-catalyzed hydrolysis of mixtures of octyltriethoxysilane and tetraethoxysilane in the presence of magnetic sporopollenin. the catalytic properties of the immobilized lipases were evaluated into model reactions, i.e., the hydrolysis of p-nitrophenylpalmitate (p-npp), and the enantioselective hydrolysis of racemic naproxen methyl ester, mandelic acid methyl ester or 2-phenoxypropionic acid methy ... | 2011 | 21932062 |
| expression and characterization of a novel propionyl-coa dehydrogenase gene from candida rugosa in pichia pastoris. | the propionyl-coa dehydrogenase (pacd) gene was firstly cloned from candida rugosa by the cdna race technique. the 6× his-tagged recombinant pacd gene was expressed in pichia pastoris gs115 and purified with ni-nta affinity chromatography. sds-page analysis and western blotting revealed that the molecular mass of the purified pacd was 49 kda. the results showed that the recombinant protein had the activity of catalyzing propionyl-coa to acrylyl-coa. the k (m), k (cat), and v (max) values of the ... | 2011 | 21960276 |
| chemoenzymatic synthesis of phytosteryl ferulates and evaluation of their antioxidant activity. | the feasibility of a two-step chemoenzymatic synthesis of phytosteryl ferulates was successfully established in this work. an intermediate vinyl ferulate was first chemically produced and subsequently esterified with phytosterols through alcoholysis with candida rugosa as a catalyst. the structures of phytosteryl ferulates were confirmed by fourier transform infrared (ftir) and high-performance chromatography-mass spectrometry/mass spectrometry (hplc-ms/ms) using atmospheric pressure chemical ... | 2011 | 22023159 |
| identification of local clinical candida isolates using chromagar candida™ as a primary identification method for various candida species. | the objective of our study was to study the effectiveness of chromagar candida™ as the primary identification method for various clinical candida isolates, other than the three suggested species by the manufacturer. we studied 34 clinical isolates which were isolated from patients in a local teaching hospital and 7 atcc strains. these strains were first cultured in sabouraud dextrose broth (sdb) for 36 hours at 35ºc, then on chromagar plates at 30ºc, 35ºc and 37ºc. the sensitivity of t ... | 2011 | 22041745 |
| Lipases are soluble and active in glycerol carbonate as a novel biosolvent. | Glycerol carbonate was synthesized as biosolvent for the development of soluble enzymatic system. The effects of various reaction parameters on activity and stability of lipases were investigated using the transesterification of ethyl butyrate with n-butanol as a model reaction. Enzymatic activity in glycerol carbonate was compared with that in water and in conventional organic solvents with different ionizing and dissociating abilities. The pK(a) value of trichloroacetic acid and transesterific ... | 2011 | 22112404 |
| mechanistic investigations of lipase-catalyzed degradation of polycarbonate in organic solvents. | the biodegradation of an engineering thermoplastic, poly (bisphenol-a carbonate) (bpapc), was carried out using three different lipases from candida antarctica (cal), candida rugosa (crl) and porcine pancreas (ppl) in water-miscible (tetrahydrofuran) and water-immiscible (chloroform) solvents for 10 days. the degradation was monitored by gel permeation chromatography and fourier transform infrared spectroscopy. maximum degradation (ca. 60% reduction in m(n)) of bpapc was observed in thf with ppl ... | 2011 | 22112773 |
| A novel control of enzymatic enantioselectivity through the racemic temperature influenced by reaction media. | The influence of reaction media on the racemic temperature (T(r)) in the lipase-catalyzed resolution of ketoprofen vinyl ester was investigated. An effective approach to the control of the enzymatic enantioselectivity and the prediction of the increasing tendency was developed based on the T(r) influenced by reaction media. The T(r) for the resolution catalyzed by Candida rugosa lipase (CRL) was found at 29 °C in aqueous and S-ketoprofen was obtained predominantly at 40 °C. However, CRL showed R ... | 2011 | 22113016 |
| Selection of orlistat as a potential inhibitor for lipase from Candida species. | Infections caused by Candida species manifest in a number of diseases, including candidemia, vulvovaginal candidiasis, endocarditis, and peritonitis. Candida species have been reported to possess lipolytic activity due to the secretion of lipolytic enzymes such as esterases, lipases and phospholipases. Extra-cellular hydrolytic enzymes seem to play an important role in Candida overgrowth. Candidiasis is commonly treated with antimycotics such as clotrimazole and nystatin. The antimycotics bind t ... | 2011 | 22125382 |
| novel magnetic microspheres of p (gma-b-hema): preparation, lipase immobilization and enzymatic activity in two phases. | magnetic oleic-acid-coated fe(3)o(4) nanoparticles were first introduced into 1, 1-diphenylethylene (dpe)-controlled radical polymerization system to prepare superparamagnetic microspheres for enzyme immobilization by two steps of polymerization. in the presence of dpe, glycidyl methacrylate, 2-hydroxyethyl methacrylate and methacryloxyethyl trimethyl ammonium chloride with charge were selected as copolymering monomers based on their reactive functional group and excellent biocompatibility which ... | 2011 | 22159608 |
| phyto-inspired silica nanowires: characterization and application in lipase immobilization. | silica nanostructures were phyto-fabricated on different surfaces by using pomegranate (punica granatum) leaf extracts. on zinc films, nanowires were obtained. on other surfaces such as silica, alumina, zinc oxide and glass, spherical aggregates, cubic assemblies, micro-flakes and acicular structures, respectively, were observed. the nanowires developed on zn surfaces were characterized by scanning electron microscope-energy dispersive spectrometer (sem-eds), x-ray diffraction (xrd), photolumin ... | 2011 | 22201456 |
| immobilization of candida rugosa lipase on hydrophobic/strong cation-exchange functional silica particles for biocatalytic synthesis of phytosterol esters. | in this work, mixed-mode silica particles functionalized with octyl and sulfonic acid groups was conveniently prepared by co-bonding a mixture of n-octyltriethoxysilane and 3-mercaptopropyltriethoxysilane and then oxidized with hydrogen peroxide. candida rugosa lipase (crl) was immobilized on the mixed-mode silica particles via hydrophobic and strong cation-exchange interaction. the resulting immobilized crl increased remarkably its stability at high temperature in comparison to free crl. the im ... | 2011 | 22209442 |
| lipase immobilization on differently functionalized vinyl-based amphiphilic polymers: influence of phase segregation on the enzyme hydrolytic activity. | microbial lipase from candida rugosa was immobilized by physical adsorption onto an ethylene-vinyl alcohol polymer (eval) functionalized with acyl chlorides. to evaluate the influence of the reagent chain-length on the amount and activity of immobilized lipase, three differently long aliphatic fatty acids were employed (c8, c12, c18), obtaining eval functionalization degrees ranging from 5% to 65%. the enzyme-polymer affinity increased with both the length of the alkyl chain and the matrix hydro ... | 2012 | 22295868 |
| high performance liquid chromatography determination of fatty acids in drying oils following lipase action. | this paper describes a quantitative analytical procedure to determine the fatty acid composition in drying oils like linseed, walnut and poppy seed. the procedure required the enzymatic hydrolysis of the oil triacylglycerol families by the action of candida rugosa lipase. the fatty acids (ffas) produced (linolenic, myristic, linoleic, palmitic, oleic and stearic) were extracted with n-heptane and derivatized with α-bromoacetophenone. their separation and quantitative determination were performed ... | 2012 | 22368115 |
| enhancement of the activity and enantioselectivity of lipase by sol-gel encapsulation immobilization onto β-cyclodextrin-based polymer. | candida rugosa lipase was encapsulated within a chemically inert sol-gel support prepared by polycondensation with tetraethoxysilane and octyltriethoxysilane in the presence of β-cyclodextrin-based polymer. the catalytic activity of the encapsulated lipases was evaluated both in the hydrolysis of p-nitrophenylpalmitate and the enantioselective hydrolysis of racemic naproxen methyl ester. it has been observed that the percent activity yield of the encapsulated lipase was 65 u/g, which is 7.5 time ... | 2012 | 22383051 |
| heterologous expression systems for lipases: a review. | the production of heterologous lipases is one of the most promising strategies to increase the productivity of the bioprocesses and to reduce costs, with the final objective that more industrial lipase applications could be implemented. in this chapter, an overview of the most common microbial expression systems for the overproduction of microbial lipases is presented. prokaryotic system as escherichia coli and eukaryotic systems as saccharomyces cerevisiae and pichia pastoris are analyzed and c ... | 2012 | 22426719 |
| first case report of bloodstream infection due to a candida species closely related to the novel species candida pseudorugosa. | candida pseudorugosa is a novel species closely related to candida rugosa for which only one case has been reported. we report the first case of a bloodstream infection in humans caused by a candida sp. closely related to c. pseudorugosa. we contribute evidence to show this organism as a potential human pathogen that may be misidentified by conventional methods, also pointing out its lower sensitivity to azoles and other antifungal agents. | 2012 | 22461681 |
| enzyme catalysis in organic solvents: influence of water content, solvent composition and temperature on candida rugosa lipase catalyzed transesterification. | in the present study the influence of water content, solvent composition and reaction temperature on the transesterification of 1-phenylpropan-2-ol catalyzed by candida rugosa lipase was examined. reactions were carried out in different mixtures of hexane and tetrahydrofurane. the studies showed that an increasing water content of the organic solvent results in an increasing enzyme activity and a decreasing enantiomeric excess. furthermore, a significant influence of the solvent hydrophilicity b ... | 2012 | 22465292 |
| molecular identification and antifungal susceptibility testing of clinical isolates of the candida rugosa species complex and proposal of the new species candida neorugosa. | candida rugosa is a poorly known fungal species occasionally involved in human infections. a molecular analysis of the sequences of the d1/d2 domains and the internal transcribed spacer (its) region of the ribosomal genes of 24 clinical isolates phenotypically identified as c. rugosa demonstrated that only 10 (41.6%) isolates belonged to that species. the other isolates were identified as candida pararugosa (41.6%) and candida pseudorugosa (8.3%). the remaining two isolates, from human and equin ... | 2012 | 22553236 |
| resonance energy transfer between protein and rhamnolipid capped zns quantum dots: application in in-gel staining of proteins. | the interaction of proteins with quantum dots is an interesting field of research. these interactions occur at the nanoscale. we have probed the interaction of bovine serum albumin (bsa) and candida rugosa lipase (crl) with rhamnolipid capped zns (rhlznsqds) using absorption and fluorescence spectroscopy. optical studies on mixtures of rhlznsqds and proteins resulted in förster's resonance energy transfer (fret) from proteins to qds. this phenomenon has been exploited to detect proteins in agaro ... | 2012 | 22580141 |
| role of plasmid in diesel oil degradation by yeast species isolated from petroleum hydrocarbon-contaminated soil. | five yeast species, namely candida tropicalis, cryptococcus laurentii, trichosporon asahii, rhodotorula mucilaginosa and candida rugosa isolated from hydrocarbon-contaminated soil were found to be potent degraders of diesel oil. these microorganisms showed the presence of enzymes cytochrome p450, nadph cytochrome c reductase, aminopyrine n demethylase, alcohol dehydrogenase, aldehyde dehydrogenase, naphthalene dioxygenase, catalase and glutathione s transferase when the cells were incubated for ... | 2012 | 22629639 |
| controlled release of lipase from candida rugosa loaded into hydrogels of n-isopropylacrylamide and itaconic acid. | the series of poly(n-isopropylacrylamide-co-itaconic acid) hydrogels, with lipase from candida rugosa as a model protein, were synthesized by free radical copolymerization. the composition of hydrogels was varied by monomers ratio, crosslinking agent concentration and amounts of lipase, which was loaded by in situ polymerization. all samples were characterized regarding morphology. the investigation of hydrogel swelling properties revealed their ph and temperature sensitive character. protein lo ... | 2012 | 22759642 |
| preparation and characterization of gelatin hydrogel support for immobilization of candida rugosa lipase. | a series of gelatin hydrogels were prepared by crosslinking method using glutaraldehyde (ga). the hydrogels were characterized by gel formation, swelling/degradation tests, and ftir analysis. the variations of swelling percentages (s%) with time, temperature, and ph were determined. it is found that the increasing amount of ga causes the decreasing in s% values from 366 to 213% and g-1 was found to be the most swollen hydrogel at ph 7.4 and 37 °c. degradation tests of hydrogel samples were carri ... | 2013 | 22812721 |
| production of biodiesel from plant oil hydrolysates using an aspergillus oryzae whole-cell biocatalyst highly expressing candida antarctica lipase b. | for enzymatic biodiesel production from plant oil hydrolysates, an aspergillus oryzae whole-cell biocatalyst that expresses candida antarctica lipase b (r-calb) with high esterification activity was developed. each of soybean and palm oils was hydrolyzed using candida rugosa lipase, and the resultant hydrolysates were subjected to esterification where immobilized r-calb was used as a catalyst. in esterification, r-calb afforded a methyl ester content of more than 90% after 6 h with the addition ... | 2013 | 22850174 |
| lipase incorporated ionic liquid polymers as active, stable and reusable biocatalysts. | the aim of this study was to develop ionic liquid (il) polymer materials incorporating enzymes that can be used as active, stable and reusable biocatalysts. to this goal, candida rugosa lipase has been microencapsulated in surfactant aggregates formed in an il monomer or the solution of an il monomer/il and then incorporated into polymer frameworks through the free radical polymerization of an il (1-vinyl-3-ethylimidazolium bis(trifluoromethyl-sulfonyl) amide) ([veim][tf(2)n]). the activity, sta ... | 2012 | 22903458 |
| rapid identification of yeast isolates from clinical specimens in critically ill trauma icu patients. | the purpose was to evaluate the performance of a commercially available chromogenic candida speciation media and the vitek 2 id system for the identification of medically important yeasts and yeast-like organisms in a routine clinical microbiology laboratory. | 2012 | 22923919 |
| candida mesorugosa sp. nov., a novel yeast species similar to candida rugosa, isolated from a tertiary hospital in brazil. | candida rugosa is a yeast species that is emerging as a causative agent of invasive infection, particularly in latin america. recently, c. pseudorugosa was proposed as a new species closely related to c. rugosa. we evaluated in this investigation the genetic heterogeneity within the c. rugosa species complex. all clinical isolates used in this study were identified phenotypically as c. rugosa but were genotypically different from the c. rugosa type, atcc 10571. rapd marker analysis revealed less ... | 2013 | 22928924 |
| immobilization of lipases on alkyl silane modified magnetic nanoparticles: effect of alkyl chain length on enzyme activity. | biocatalytic processes often require a full recycling of biocatalysts to optimize economic benefits and minimize waste disposal. immobilization of biocatalysts onto particulate carriers has been widely explored as an option to meet these requirements. however, surface properties often affect the amount of biocatalysts immobilized, their bioactivity and stability, hampering their wide applications. the aim of this work is to explore how immobilization of lipases onto magnetite nanoparticles affec ... | 2012 | 22952688 |
| immobilization of candida rugosa lipase on mcm-41 for the transesterification of cotton seed oil. | present study demonstrated the preparation of mcm-41 as a support for the immobilization of candida rugosa lipase by the physical adsorption technique. the lipase immobilized mcm-41 has been characterized by scanning electron microscopic and ftir techniques. at ph 6, maximum lipase immobilization (250 mg/g) on mcm support has been observed and the immobilized lipase was employed as biocatalyst for the transesterification of the cotton seed oil with methanol. the ph of the reaction medium, reacti ... | 2012 | 22975780 |
| isoamylacetate production by entrapped and covalently bound candida rugosa and porcine pancreatic lipases. | candida rugosa lipase (crl) and porcine pancreatic lipase (ppl) were immobilised by entrapping and also by covalent binding for use in synthesis of isoamyl acetate (iaac), which has a typical banana flavour. lipase entrapment was carried out by dripping sodium alginate (na-alg)-chitosan (chi)-lipase mixture into cacl(2)-glutaraldehyde (gal) solution to obtain ca-alg/chi(crl/ppl). immobilisation conditions were optimised as 1.5% na-alg, 1.5% chitosan and 0.15% gal. ca-alg/chi(crl/ppl) samples sho ... | 2012 | 22980809 |
| preparation of superparamagnetic fe3o4@alginate/chitosan nanospheres for candida rugosa lipase immobilization and utilization of layer-by-layer assembly to enhance the stability of immobilized lipase. | superparamagnetic alginate nanospheres with diameter of 50 nm were prepared by self-assembly of alginate in the ca(2+) solution; and then superparamagnetic alginate/chitosan nanospheres, which have positive charge and could adsorb lipase directly, were obtained with a following assembly of chitosan based on the electrostatic interaction between alginate and chitosan. subsequently, oxidic poly (ethylene glycol) was used to functionalize the magnetic alginate/chitosan nanospheres. thus, the magnet ... | 2012 | 22985256 |
| effect of poly(vinyl acetate-acrylamide) microspheres properties and steric hindrance on the immobilization of candida rugosa lipase. | poly(vinyl acetate-acrylamide) microspheres were synthesized in the absence or presence of isooctane via suspension polymerization and utilized as carriers to immobilize candida rugosa lipase. when the hydrophobic/hydrophilic surface characteristics of the microspheres were modified by changing the ratio of vinyl acetate (hydrophobic monomer) to acrylamide (hydrophilic monomer) from 50:50 to 86:24, the immobilization ratio changed from 45% to 92% and the activity of the immobilized lipase increa ... | 2012 | 22989650 |
| evaluation of structure and hydrolysis activity of candida rugosa lip7 in presence of sub-/super-critical co₂. | this work aimed to assess the effect of sub-/super-critical co₂ on the structure and activity of candida rugosa lip7 (crl7) in its solution form. the structure was examined by sds-page gel electrophoresis, circular dichroism (cd) and fluorescence spectra photometry. results revealed that the primary structure remained intact after sub-/super-critical co₂ treatment, and the secondary structure altered at the pressure of 10 mpa and temperature 40°c for 30 min incubation, but it was reflex to its n ... | 2012 | 23040391 |
| biodiversity and probiotic potential of yeasts isolated from fura, a west african spontaneously fermented cereal. | fura is a spontaneously fermented pearl millet product consumed in west africa. the yeast species involved in the fermentation were identified by pheno- and genotypic methods to be candida krusei, kluyveromyces marxianus, candida tropicalis, candida rugosa, candida fabianii, candida norvegensis and trichosporon asahii. c. krusei and k. marxianus were found to be the dominant species. survival in ph 2.5 or in the presence of bile salts (0.3% (w/v) oxgall) and growth at 37°c were independently det ... | 2012 | 23072700 |
| magnetically modified spent grain as a low-cost, biocompatible and smart carrier for enzyme immobilisation. | food and feed technology and biotechnology benefit from the use of immobilised enzymes. new low-cost enzyme carriers exhibiting high biocompatibility and response to external magnetic field can substantially improve the application potential of immobilised enzyme systems. | 2013 | 23108838 |
| lipase immobilization on hyper-cross-linked polymer-coated silica for biocatalytic synthesis of phytosterol esters with controllable fatty acid composition. | in this study, a novel mixed-mode composite material, sio(2)@p(maa-co-vbc-co-dvb), was prepared via the hyper-cross-linking of its precursor, which was produced via suspension polymerization in the presence of sio(2) particles. candida rugosa lipase (crl) was immobilized on the sio(2)@p(maa-co-vbc-co-dvb) particles via hydrophobic and weak cation-exchange interaction. the resulting immobilized crl showed much better thermal stability and reusability in comparison to free crl. on the basis of the ... | 2013 | 23199227 |
| preparation of coaxial-electrospun poly[bis(p-methylphenoxy)]phosphazene nanofiber membrane for enzyme immobilization. | a core/sheath nanofiber membrane with poly[bis(p-methylphenoxy)]phospha-zene (pmpph) as the sheath and easily spinnable polyacrylonitrile (pan) as the core was prepared via a coaxial electrospinning process. field-emission scanning electron microscopy and transmission electron microscopy were used to characterize the morphology of the nanofiber membrane. it was found that the concentration of the pan spinning solution and the ratio of the core/sheath solution flow rates played a decisive role in ... | 2012 | 23203055 |
| comparison of artificial neural network (ann) and response surface methodology (rsm) in optimization of the immobilization conditions for lipase from candida rugosa on amberjet(®) 4200-cl. | candida rugosa lipase (crl) is an important industrial enzyme that is successfully utilized in a variety of hydrolysis and esterification reactions. this work describes the optimization of immobilization conditions (enzyme/support ratio, immobilization temperature, and buffer concentration) of crl on the anionic resin amberjet® 4200-cl, using enantioselectivity (e) as the reference parameter. the model reaction used for this purpose is the acylation of (r,s)-1-phenylethanol. optimal conditions f ... | 2013 | 23215653 |
| lipase catalyzed ultrasonic synthesis of poly-4-hydroxybutyrate-co-6-hydroxyhexanoate. | four different lipases were compared for ultrasound-mediated synthesis of the biodegradable copolymer poly-4-hydroxybutyrate-co-6-hydroxyhexanoate. the copolymerization was carried out in chloroform. of the enzymes tested, novozym 435 exhibited the highest copolymerization rate, in fact the reaction rate was observed to increase with about 26-fold from 30 to 50°c (7.9×10(-3)ms(-1)), sonic power intensity of 2.6×10(3)wm(-2) and dissipated energy of 130.4jml(-1). copolymerization rates with the ca ... | 2013 | 23231942 |
| extracellular enzyme production and phylogenetic distribution of yeasts in wastewater treatment systems. | the abilities of yeasts to produce different extracellular enzymes and their distribution characteristics were studied in municipal, inosine fermentation, papermaking, antibiotic fermentation, and printing and dyeing wastewater treatment systems. the results indicated that of the 257 yeasts, 16, 14, 55, and 11 produced lipase, protease, manganese dependant peroxidase (mnp), and lignin peroxidase (lip), respectively. they were distributed in 12 identified and four unidentified genera, in which ca ... | 2013 | 23261999 |
| biochemical methane potential of raw and pre-treated meat-processing wastes. | raw and pre-treated greaves and rinds, two meat-processing wastes, were assessed for biochemical methane potential (bmp). combinations of temperature (25, 55, 70 and 120 °c), naoh (0.3 g g(-1) waste volatile solids) and lipase from candida rugosa (10 u g(-1) fat) were applied to promote wastes hydrolysis, and the effect on bmp was evaluated. cod solubilisation was higher (66% for greaves; 55% for rinds) when greaves were pre-treated with naoh at 55 °c and lipase was added to rinds after autoclav ... | 2012 | 23266854 |
| in silico and experimental characterization of chimeric bacillus thermocatenulatus lipase with the complete conserved pentapeptide of candida rugosa lipase. | lipases are one of the highest value commercial enzymes as they have broad applications in detergent, food, pharmaceutical, and dairy industries. to provide chimeric bacillus thermocatenulatus lipase (btl2), the completely conserved pentapeptide (¹¹²ala-his-ser-gln-gly¹¹⁶) was replaced with similar sequences (²⁰⁷gly-glu-ser-ala-gly²¹¹) of candida rugosa lipase (clr) at the nucleophilic elbow region. for this purpose, three mutations including a112g, h113e, and q115a were inserted in the conserve ... | 2012 | 23274720 |
| lipase-immobilized biocatalytic membranes for biodiesel production. | microbial lipase from candida rugosa (amano ay-30) has good transesterification activity and can be used for biodiesel production. in this study, polyvinylidene fluoride (pvdf) membrane was grafted with 1,4-diaminobutane and activated by glutaraldehyde for c. rugosa lipase immobilization. after immobilization, the biocatalytic membrane was used for producing biodiesel from soybean oil and methanol via transesterification. response surface methodology (rsm) in combination with a 5-level-5-factor ... | 2012 | 23357586 |
| pcr-sscp-based reconstruction of the original fungal flora of heat-processed meat products. | food processing of spoiled meat is prohibited by law, since it is a deception and does not comply with food safety aspects. in general, spoilage of meat is mostly caused by bacteria. however, a high contamination level of fungi could be also found in some meat or meat products with certain preserving conditions. in case that unhygienic meat is used to produce heat processed products, the microorganisms will be deactivated by heat, so that they cannot be detected by a standard cultivation method. ... | 2013 | 23361099 |
| structural and dynamic features of candida rugosa lipase 1 in water, octane, toluene, and ionic liquids bmim-pf6 and bmim-no3. | ionic liquids (ils) and organic chemicals can be used as solvents in biochemical reactions to influence the structural and dynamic features of the enzyme, sometimes detrimentally. in this work we report the results for molecular dynamics simulations of candida rugosa lipase (crl) in ils bmim-pf6 and bmim-no3, as well as organic solvents toluene and octane in an effort to explore the role of solvent on the structure and dynamics of an enzyme known to be active in many nonaqueous media. simulation ... | 2013 | 23387335 |
| assessment of activities and conformation of lipases treated with sub- and supercritical carbon dioxide. | in order to illustrate the underlining mechanism of the effect of high pressure on lipases from different resources, the influence of compressed carbon dioxide treatment on the esterification activities and conformation of the three lipases candida rugosa lipase (crl), pseudomonas fluorescens lipase, and rhizopus oryzae lipase was investigated in the present work. the results showed that the lipases activities were significantly enhanced in most of high-pressure treatments, except the pressure h ... | 2013 | 23417391 |
| promotion of the lipase-catalyzed hydrolysis of conjugated linoleic acid l-menthyl ester by addition of an organic solvent. | conjugated linoleic acid l-menthyl ester was hydrolyzed in water by the lipase from candida rugosa with the addition of an organic solvent. the degree of hydrolysis (yield) greatly improved when a tertiary alcohol, such as t-butyl alcohol, was added. however, the addition of a less polar solvent, such as hexane, decreased the degree of hydrolysis, and some water-miscible solvents, such as acetone, caused inactivation of the lipase. with the addition of t-butyl alcohol, the reaction mixture forme ... | 2012 | 23420098 |
| a magnetically separable biocatalyst for resolution of racemic naproxen methyl ester. | candida rugosa lipase (crl) was encapsulated via the sol-gel method, using 5, 11, 17, 23-tetra-tert-butyl-25,27-bis(2-aminopyridine)carbonylmethoxy-26, 28-dihydroxy-calix[4]arene-grafted magnetic fe3o4 nanoparticles (calix-m-e). the catalytic activity of encapsulated lipase (calix-m-e) was tested both in the hydrolysis of p-nitrophenyl palmitate (p-npp) and the enantioselective hydrolysis of racemic naproxen methyl ester. the present study demonstrated that the calixarene-based compound has the ... | 2013 | 23525833 |
| taxonomic and molecular characterization of lactic acid bacteria and yeasts in nunu, a ghanaian fermented milk product. | produced from raw unpasteurized milk, nunu is a spontaneously fermented yoghurt-like product made in ghana and other parts of west africa. despite the importance of nunu in the diet of many africans, there is currently only limited information available on the microorganisms associated with nunu processing. with the aim of obtaining a deeper understanding of the process and as a first step towards developing starter cultures with desired technological properties for nunu production, a microbiolo ... | 2012 | 23541194 |
| isolation, identification and growth determination of lactic acid-utilizing yeasts from the ruminal fluid of dairy cattle. | ruminal organic acid production, especially lactic acid, can be modified by feeding cattle highly concentrated diets, which have been shown to adversely affect dairy cattle health. therefore, the use of lactic acid-utilizing organisms is considered to be a potential method for controlling lactic acid levels. this study was conducted to isolate and identify lactic acid-utilizing yeasts from the ruminal fluid of dairy cattle and to determine the specific growth rate and generation time when using ... | 2013 | 23565722 |
| covalent immobilization of candida rugosa lipase on aldehyde functionalized hydrophobic support and the application for synthesis of oleic acid ester. | this study focuses on candida rugosa lipase (crl) immobilization by covalent attachment on poly(ethylene terephthalate)-grafted glycidyl methacrylate (pet-g-gma) fiber. the immobilization yielded a protein loading of 2.38 mg g(-1) of pet-g-gma fiber. the performances of the immobilized and free crls were evaluated with regard to hydrolysis of olive oil and esterification of oleic acid. the optimum activity ph of the crl was changed by immobilization to neutral range. the maximum activity of the ... | 2013 | 23574345 |
| one-pot solvothermal synthesis of highly water-dispersible size-tunable functionalized magnetite nanocrystal clusters for lipase immobilization. | a facile one-pot synthesis of highly water-dispersible size-tunable magnetite (fe3o4) nanocrystal clusters (mncs) end-functionalized with amino or carboxyl groups by a modified solvothermal reduction reaction has been developed. dopamine and 3,4-dihydroxyhydroxycinnamic acid were used for the first time as both a surfactant and interparticle linker in a polylol process for economical and environment-friendly purposes. morphology, chemical composition, and magnetic properties of the prepared part ... | 2013 | 23616374 |
| synthesis, enantiomer separation, and absolute configuration of 2,6-oxygenated 9-azabicyclo[3.3.1]nonanes. | the synthesis of the enantiomerically pure n-boc 9-azabicyclo[3.3.1]nonane-2,6-dione (4b), a potentially useful chiral building block, from n-bn and n-boc 9-azabicyclo[3.3.1]nonane-2,6-diols 2a and 2b was accomplished. the enantiomer resolution of diols 2a and 2b was achieved by crystallization of their diastereomeric esters or by kinetic resolution of the racemic diol 2a using lipase from candida rugosa (crl). both enantiomers of n-boc protected diol 2b were converted into the corresponding ena ... | 2013 | 23635000 |
| the derivation of a chiral substituent code for secondary alcohols and its application to the prediction of enantioselectivity. | a chiral substituent code was proposed based on the features of secondary alcohols, in which a chiral center is attached to two substituents in addition to oh and h substituents. the new chirality code, which was generated by predefining positional information of four substituents attached to stereocenter, was applied to two datasets composed of secondary alcohols as the enantioselective products of asymmetric reactions. in the first dataset, the chemical reaction was catalyzed by a biocatalyst, ... | 2013 | 23666031 |
| determination of yeast diversity in ogi, mawè, gowé and tchoukoutou by using culture-dependent and -independent methods. | the maize based ogi and mawè and the sorghum based gowé and tchoukoutou are traditional, spontaneously fermented products widely consumed by the population of benin (west africa). yeast occurrence in the products, as sold on local markets at different locations, was studied using a combination of culture-dependent and independent methods. number of yeasts is varied from 3.75 log10 colony forming units (cfu)/g for ogi to 5.60 log10 cfu/g for tchoukoutou. isolated yeasts (236) were identified base ... | 2013 | 23727651 |
| lipases efficiently stearate and cutinases acetylate the surface of arabinoxylan films. | this is the first report on successful enzyme catalyzed surface esterification of hemicellulose films. enzyme catalyzed surface acetylation with vinyl acetate and stearation with vinyl stearate were studied on rye arabinoxylan (ax) films. different surface analytical techniques (ft-ir, tof-sims, esca, ca) show that lipases from mucor javanicus, rhizopus oryzae and candida rugosa successfully surface stearate ax films and that a cutinase from fusarium solani pisi surface acetylates these films. t ... | 2013 | 23774036 |
| preparation of new calix[4]arene-immobilized biopolymers for enhancing catalytic properties of candida rugosa lipase by sol-gel encapsulation. | the article describes preparation of new calixarene biopolymers consisting of the immobilization of convenience calixarene derivative onto cellulose and chitosan biopolymers, and the encapsulation of these calixarene biopolymers with candida rugosa lipase within a chemical inert sol-gel supported by polycondensation with tetraethoxysilane and octyltriethoxysilane. the catalytic properties of immobilized lipase were evaluated into model reactions employing the hydrolysis of p-nitrophenylpalmitate ... | 2013 | 23780340 |
| survey of mycotic mastitis in dairy cows from heilongjiang province, china. | a survey of the prevalence rate, pathogenic subspecies, and risk factors of mycotic mastitis in dairy cows from heilongjiang province, china, was conducted. milk samples from 412 cows with chronic mastitis were collected and cultured on 8 % sheep blood agar, macconkey agar, and sabouraud agar with chloramphenicol. counting of the morphologically distinct colonies was performed, as well as the isolation and identification of organisms through phenotypical and physiological criteria. four hundred ... | 2013 | 23813119 |
| immobilization of candida rugosa lipase on superparamagnetic fe3o4 nanoparticles for biocatalysis in low-water media. | a simple immobilization method for candida rugosa lipase on superparamagnetic fe3o4 nanoparticles is described. the fe3o4 nanoparticles were coated with pei and candida rugosa lipase was adsorbed on these particles via electrostatic interactions. the immobilization resulted in marginal simultaneous purification. however, the immobilized preparation showed 110× higher transesterification activity in low-water media. it was also efficient in kinetic resolution of (±)-1-phenylethanol with eep of 99 ... | 2013 | 23934801 |
| demonic axe-like conjugated alkynes in combating microbes. | a new series of disubstituted alkynes was obtained by microwave induced internal splitting of the corresponding β-oxo-alkylidenetriphenylphosphoranes. the antimicrobial potential of these conjugated alkynes and phosphoranes was assayed in vitro against three gram-positive bacteria (staphylococcus aureus, bacillus subtilis, staphylococcus epidermidis), three gram-negative bacteria (escherichia coli, pseudomonas aeruginosa, klebsiella pneumoniae) and five fungal strains (aspergillus niger, candida ... | 2013 | 23973825 |
| synthesis of phytosteryl ester containing pinolenic acid in a solvent-free system using immobilized candida rugosa lipase. | phytosteryl ester synthesized with pinolenic acid (pla) from pine nut oil is expected to have features of both phytosterol and pla. in this study, lipase from candida rugosa (crl) was immobilized and then used to optimize conditions for synthesis of phytosteryl ester containing pla. lewatit vp oc 1600, a macroporous hydrophobic resin, was selected as the best carrier, and the optimum condition for the immobilization of crl was established. with immobilized crl prepared, synthesis of phytosteryl ... | 2013 | 23988007 |
| biosynthesis of sucrose-6-acetate catalyzed by surfactant-coated candida rugosa lipase immobilized on sol-gel supports. | sucrose-6-acetate is an important intermediate in the preparation of sucralose (a finest sweetener). in our study, candida rugosa lipase coated with surfactant was firstly immobilized on sol-gel supports. then, the immobilized enzyme was used in the regioselective synthesis of sucrose-6-acetate by transesterification of sucrose and vinyl acetate. the screening results revealed that tween 80 was an ideal surfactant to coat lipase immobilized in sol-gel and exhibited the highest yield of sucrose-6 ... | 2014 | 24037039 |
| improving catalytic hydrolysis reaction efficiency of sol-gel-encapsulated candida rugosa lipase with magnetic β-cyclodextrin nanoparticles. | a silica-based β-cyclodextrin was immobilized on magnetic nanoparticles to obtain a macrocyclic compound with magnetic property. then, the β-cyclodextrin-grafted magnetic nanoparticles were encapsulated with candida rugosa lipase in sol-gel matrices using alkoxysilane precursors. the catalytic activity of the encapsulated lipases was evaluated with model reactions, i.e., the hydrolysis of p-nitro-phenylpalmitate (p-npp) and the enantioselective hydrolysis of rasemic naproxen methyl ester that wa ... | 2014 | 24090713 |
| calix[n]arene carboxylic acid derivatives as regulators of enzymatic reactions: enhanced enantioselectivity in lipase-catalyzed hydrolysis of (r/s)-naproxen methyl ester. | candida rugosa lipase was immobilized with a sol-gel encapsulation procedure in the presence and absence of a calix[n]arene carboxylic acid derivative grafted onto magnetic nanoparticles or in the presence of the calix[n]arene carboxylic acid derivative with fe3o4 magnetic nanoparticles as an additive. through the enantioselective hydrolysis of racemic naproxen methyl ester and the hydrolysis of p-nitrophenylpalmitate, the relative enzyme activity was evaluated and tested. these results show tha ... | 2014 | 24092454 |
| two-step method of enzymatic synthesis of starch laurate in ionic liquids. | enzymatic esterification of starch with long-chain fatty acid was investigated by using ionic liquids 1-butyl-3-methylimidazolium choride ([bmim]cl) and 1-butyl-3-methylimidazolium tetrafluoroborate ([bmim][bf4]) as reaction media. an industrial lipase produced by candida rugosa was used to modify starch with lauric acid. the effect of reaction parameters such as the lipase dosage, the molar ratio of lauric acid/anhydroglucose unit (agu) in starch, and the reaction temperature as well as the rea ... | 2013 | 24099559 |
| fungal genomes mining to discover novel sterol esterases and lipases as catalysts. | sterol esterases and lipases are enzymes able to efficiently catalyze synthesis and hydrolysis reactions of both sterol esters and triglycerides and due to their versatility could be widely used in different industrial applications. lipases with this ability have been reported in the yeast candida rugosa that secretes several extracellular enzymes with a high level of sequence identity, although different substrate specificity. this versatility has also been found in the sterol esterases from th ... | 2013 | 24138290 |
| systematic review and new insights into the molecular characterization of the candida rugosa species complex. | recently, candida rugosa was characterized as a species complex comprising four taxa: c. rugosa sensu stricto, candida pseudorugosa, candida neorugosa and candida mesorugosa. although considered relatively rare, several clusters of candidemia due to c. rugosa complex had been reported presenting mortality rates close to 70%. in this work we discuss the systematization, phenotyping and molecular methods based on internal transcribed spacer region (its) sequencing and proteomic analyses for specie ... | 2013 | 24161727 |
| effect of metal ions on the hydrolytic and transesterification activities of candida rugosa lipase. | in order to study the effect of metal ions on lipase activity, hydrolytic and transesterification activities of candida rugosa lipase were investigated in presence of alkali (na⁺ and k⁺), alkaline earth (ca⁺² and ba⁺²) and transition (cr⁺³, fe⁺³, co⁺², cu⁺² and ni⁺²) metal ions. maximum enhancement in hydrolytic activity of lipase was observed by ca⁺², and in transesterification activity by cr⁺³ and co⁺². the kinetics of the lipase catalyzed transesterification (methanolysis and ethanolysis) rea ... | 2013 | 24200940 |
| conformation and catalytic properties studies of candida rugosa lip7 via enantioselective esterification of ibuprofen in organic solvents and ionic liquids. | enantioselective esterification of ibuprofen was conducted to evaluate the enzyme activity and ees of lipase from candida rugosa (crl7) in ten conventional organic solvents and three ionic liquids. different alcohols were tested for selecting the most suitable acyl acceptor due to the fact that the structure of alcohols (branch and length of carbon chains; location of -oh functional group) could affect the enzyme activity and ees. the results of alcohol and solvent selection revealed that 1-isoo ... | 2013 | 24381516 |
| removal of zn(ii) from electroplating effluent using yeast biofilm formed on gravels: batch and column studies. | present study deals with the removal of zn(ii) ions from effluent using yeast biofilm formed on gravels. | 2014 | 24397917 |
| study of different reaction schemes for the enzymatic synthesis of polyglycerol polyricinoleate. | different strategies for the solvent-free enzymatic production of polyglycerol polyricinoleate (pgpr) were explored in an attempt to simplify and improve the process. besides the conventional procedure (obtaining polyricinoleic acid, followed by its esterification with polyglycerol), two alternative methods are proposed: (1) reversing the synthesis order, i.e. esterification of polyglycerol with ricinoleic acid and then the condensation of ricinoleic acid with the previously obtained polyglycero ... | 2014 | 24403129 |
| highly stabilized lipase in polyaniline nanofibers for surfactant-mediated esterification of ibuprofen. | lipase (lp) from candida rugosa was immobilized and stabilized in polyaniline nanofibers (panfs) via a three-step process of enzyme adsorption, precipitation, and cross-linking, which generates the final immobilization called "eapc". the activity of eapc was 5.1 and 5.9 times higher than those of lp immobilizations via enzyme adsorption (ea) and enzyme adsorption/cross-linking (eac), respectively. after incubation in an aqueous buffer under shaking (200 rpm) for 84 days, eapc maintained 74% of i ... | 2014 | 24417226 |
| diversity of culturable yeasts in phylloplane of sugarcane in thailand and their capability to produce indole-3-acetic acid. | yeasts were isolated by the enrichment technique from the phylloplane of 94 samples of sugarcane leaf collected from seven provinces in thailand. all sugarcane leaf samples contained yeasts and 158 yeast strains were obtained. on the basis of the d1/d2 domain of the large subunit rrna gene sequence analysis, 144 strains were identified to 24 known species in 14 genera belonging to the ascomycota viz. candida akabanensis, candida dendronema, candida mesorugosa, candida michaelii, candida nivarien ... | 2014 | 24442819 |