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a hemolysin from the mushroom pleurotus eryngii.a monomeric 17-kda hemolysin designated as eryngeolysin was isolated from fresh fruiting bodies of the mushroom pleurotus eryngii, using a protocol that involved gel filtration on superdex 75, ion exchange chromatography on mono q and gel filtration on superdex 75. its n-terminal sequence demonstrated striking homology to that of its counterparts ostreolysin from the oyster mushroom pleurotus ostreatus and aegerolysin from the mushroom agrocybe cylindracea. its hemolytic activity was unaffected ...200616804695
exclusive overproduction of recombinant versatile peroxidase mnp2 by genetically modified white rot fungus, pleurotus ostreatus.by combining a homologous recombinant gene expression system and optimization of the culture conditions, hyper overproduction of pleurtous ostreatus mnp2 was achieved. genetically modified p. ostreatus strains with the recombinant mnp2 sequence under the control of sdi1 expression signals, were subjected to agitated culture using media supplemented with wheat bran or its hot-water extract. the best result, whereby 7300 u/l of mnp was produced by a recombinant strain tm2-18, indicated that more t ...200616820241
a bacterium belonging to the burkholderia cepacia complex associated with pleurotus ostreatus.pleurotus ostreatus is a widely cultivated white-rot fungus. owing to its considerable enzymatic versatility p. ostreatus has become the focus of increasing attention for its possible utility in biobleaching and bioremediation applications. interactions between microorganisms can be an important factor in those processes. in this study, we describe the presence of a bacterial species associated with p. ostreatus strain g2. this bacterial species grew slowly (approximately 30 days) in the liquid ...200616820755
an evaluation of soil colonisation potential of selected fungi and their production of ligninolytic enzymes for use in soil bioremediation applications.initially sixteen fungi were screened for potential ligninolytic activity using decolourisation of a polymeric dye poly r-478. from this, four fungi were selected, trametes versicolor, pleurotus ostreatus, collybia sp., and an isolate (identified as rhizoctonia solani) isolated from a grassland soil. differences in the ligninolytic enzyme profiles of each of the fungi were observed. all of the four fungi tested produced mnp and laccase while the collybia sp. and r. solani produced lip in additio ...200616820969
cytotoxic effect of oyster mushroom pleurotus ostreatus on human androgen-independent prostate cancer pc-3 cells.twenty species of edible mushrooms and three purified mushroom polysaccharides were screened for their antitumor potential on human androgen-independent cancer pc-3 cells. a water-soluble extract (poe) prepared from the fresh oyster mushroom pleurotus ostreatus produced the most significant cytotoxicity on pc-3 cells among the mushroom species tested. at the same time, poe induced a rapid apoptosis on pc-3 cells detected with annexin v-fluorescein isothiocyanate flow cytometry when the cells wer ...200616822205
rate of iodine volatilization and accumulation by filamentous fungi through laboratory cultures.five strains of basidiomycetes (lentinula edodes, coprinus phlyctidosporus, hebeloma vinosophyllum, pleurotus ostreatus and agaricus bisporus), one strain of ascomycete (hormoconis resinae) and six strains of imperfect fungi (penicillium chrysogenum, penicillium roquefortii, cladosporium cladosporioides, alternaria alternata, aspergillus niger and aspergillus oryzae) were cultured in a liquid medium containing a radioactive iodine tracer ((125)i), and were tested for their abilities to volatiliz ...200616828143
application of dna from mushroom pleurotus ostreatus for cancer biotherapy: a pilot study.in present work, the attempt to study immunomodulatory activity and biotherapeutical potential of dna isolated from the fruit body of p. ostreatus was made.200616837904
steroid structural requirements for interaction of ostreolysin, a lipid-raft binding cytolysin, with lipid monolayers and bilayers.ostreolysin, a cytolytic protein from the edible oyster mushroom (pleurotus ostreatus), recognizes and binds specifically to membrane domains enriched in cholesterol and sphingomyelin (or saturated phosphatidylcholine). these events, leading to permeabilization of the membrane, suggest that a cholesterol-rich liquid-ordered membrane phase, which is characteristic of lipid rafts, could be its possible binding site. in this work, we present effects of ostreolysin on membranes containing various st ...200616857161
toxic and lethal effects of ostreolysin, a cytolytic protein from edible oyster mushroom (pleurotus ostreatus), in rodents.ostreolysin (oly), an acidic, 15 kda protein from the edible oyster mushroom (pleurotus ostreatus), is a toxic, pore-forming cytolysin. in this paper, its toxic properties have been studied in rodents and the ld(50) in mice shown to be 1170 microg/kg. electrocardiogram, arterial blood pressure and respiratory activity were recorded under general anaesthesia, in intact, pharmacologically vagotomised and artificially respirated rats injected with one mouse ld(50). a few seconds after intravenous o ...200616860832
xyloglucanases in the interaction between saprobe fungi and the arbuscular mycorrhizal fungus glomus mosseae.we studied the production of xyloglucanase enzymes of pea and lettuce roots in the presence of saprobe and arbuscular mycorrhizal (am) fungi. the am fungus glomus mosseae and the saprobe fungi fusarium graminearum, fusarium oxysporum-126, trichoderma harzianum, penicillium chrysogenum, pleurotus ostreatus and aspergillus niger were used. g. mosseae increased the shoot and root dry weight of pea but not of lettuce. most of the saprobe fungi increased the level of mycorrhization of pea and lettuce ...200716904235
antioxidant activity of the oyster mushroom, pleurotus ostreatus, on ccl(4)-induced liver injury in rats.this study was undertaken to investigate the putative antioxidant activity of the oyster mushroom pleurotus ostreatus on ccl(4)-induced liver damage in male wistar rats. intraperitoneal administration of ccl(4) (2ml/kg) to rats for 4 days resulted in significantly elevated (p<0.05) serum levels of glutamic oxaloacetic transaminase (sgot), glutamic pyruvate transaminase (sgpt) and alkaline phosphatase (salp) compared to controls. in the liver, significantly elevated levels (p<0.05) of malondialde ...200616914248
structure-activity relationships of triterpenoid saponins on fruiting body induction in pleurotus ostreatus.to elucidate the structure-activity relationships of saponin on fruiting body induction in pleurotus ostreatus, monodesmosidic saponins comprising a triterpenoid and glucose residues were synthesized. betulin, a triterpenoid present in birch bark, was used as the aglycon. the fruiting body induction activity increased with the number of glucose residues, from one to four. the most active betulinic saponin was betulin-3-yl beta-d-(4-o-beta-d-maltotriosyl)-glucoside.200616926513
isolation of a novel mycovirus omiv in pleurotus ostreatus and its detection using a triple antibody sandwich-elisa.a novel mycovirus was isolated from a diseased mushroom, pleurotus ostreatus, using a purification procedure involving polyethylene glycol (peg)-nacl precipitation, differential centrifugation, and equilibrium centrifugation in a cscl gradient. the virion was a 43 nm isometric virus encapsulating double-stranded rna (dsrna) genome of 2.1, 2.0, 1.9, and 1.7 kbp with a coat protein (cp) of 58 kda. the new mycovirus was named oyster mushroom isometric virus (omiv). a triple antibody sandwich-elisa ...200616930731
fumigant activity of plant essential oils and components from schizonepeta tenuifolia against lycoriella ingenua (diptera: sciaridae).plant essential oils from 21 plant species were tested for their insecticidal activities against larvae of lycoriella ingenua dufour (diptera: sciaridae) by using a fumigation bioassay. good insecticidal activity against larvae of l. ingenua was achieved with essential oils of acorus gramineus solander, schizonepeta tenuifolia briquet, and zanthoxylum piperitum de candolle at 25 microg/ml air. s. tenuifolia oil showed the most potent insecticidal activity among the plant essential oils. at 12.5 ...200617066804
study of biodegradation products from azo dyes in fungal degradation by capillary electrophoresis/electrospray mass spectrometry.biodegradation products from four model sulfonated azo dyes orange ii, acid orange 8, food yellow 3, and 4-[(4-hydroxyphenyl)azo]-benzenesulfonic acid, sodium salt (4haba), during fungal degradation were determined by capillary electrophoresis coupled with ion trap mass spectrometry (ce-ms) with electrospray ionization and a coaxial sheath flow interface. the development and optimization of this analytical method including the sheath liquid composition and flow rate, nebulizing gas flow rate, ca ...200717126346
protective effect of an extract of the oyster mushroom, pleurotus ostreatus, on antioxidants of major organs of aged rats.this study was undertaken to investigate the putative antioxidant activity of the oyster mushroom, pleurotus ostreatus, on lipid peroxidation and antioxidant status of major organs of aged (24 month old) rats when compared to young (4 month old) rats. elevated levels of malondialdehyde (mda) and significantly lowered levels of reduced glutathione (gsh) and of vitamins c and e were observed in the liver, kidneys, heart and brain of aged rats, when compared to values in young rats. quantitative an ...200717126515
cultivation of pleurotus ostreatus on weed plants.oyster mushroom, pleurotus ostreatus (jacq.:fr.) kumm. itcc 3308 (collected from indian type culture collection, iari, new delhi, india, 110012) was grown on dry weed plants, leonotis sp, sida acuta, parthenium argentatum, ageratum conyzoides, cassia sophera, tephrosia purpurea and lantana camara. leonotis sp. was the best substrate in fruit body production of p. ostreatus when it was mixed with rice straw (1:1, wet wt/wet wt) for mushroom cultivation. the fruiting time for p. ostreatus was also ...200717161599
enhanced production of ligninolytic enzymes and decolorization of molasses distillery wastewater by fungi under solid state fermentation.selected isolates of fungi were grown on wheat straw and corncob in the presence of different moistening agents such as water, molasses, potato dextrose broth and distillery effluent. all the fungal isolates responded differently with respect to growth and ligninolytic enzyme production. fungal growth on different substrates was checked by calculating ergosterol content, which varied widely within a single species when grown on different substrates. the maximum laccase production was obtained fo ...200717177104
trehalose phosphorylase from pleurotus ostreatus: characterization and stabilization by covalent modification, and application for the synthesis of alpha,alpha-trehalose.trehalose phosphorylase from the basidiomycete pleurotus ostreatus (potpase) was isolated from fungal fruit bodies through approximately 500-fold purification with a yield of 44%. combined analyses by sds-page and gelfiltration show that potpase is a functional monomer of approximately 55 kda molecular mass. potpase catalyzes the phosphorolysis of alpha,alpha-trehalose, yielding alpha-d-glucose 1-phosphate (alphaglc 1-p) and alpha-d-glucose as the products. the optimum ph of potpase for alpha,al ...200717222933
feasibility of using olive mill effluent (ome) as a wetting agent during the cultivation of oyster mushroom, pleurotus ostreatus, on wheat straw.in this study, cultivation of oyster mushroom, pleurotus ostreatus, on wheat straw substrate containing tap water and olive mill effluent (ome) mixture containing varying volume of ome was studied in order to investigate the feasibility of using ome as an alternative wetting agent and ome's impact on some fundamental food quality characteristics of mushrooms. time period for mycelial colonization, primordium initiation and first harvest were comparatively evaluated with the control group. it was ...200817239585
purification of a pore-forming peptide toxin, tolaasin, produced by pseudomonas tolaasii 6264.tolaasin, a pore-forming peptide toxin, is produced by pseudomonas tolaasii and causes brown blotch disease of the cultivated mushrooms. p. tolaasii 6264 was isolated from the oyster mushroom damaged by the disease in korean. in order to isolate tolaasin molecules, the supernatant of bacterial culture was harvested at the stationary phase of growth. tolaasin was prepared by ammonium sulfate precipitation and three steps of chromatograpies, including a gel permeation and two ion exchange chromato ...200717244491
occupational asthma due to spores of pleurotus ostreatus. 200717298434
alternative medium for production of pleurotus ostreatus biomass and potential antitumor polysaccharides.pleurotus species are recognized for producing beta-glucans with important medicinal properties as a constituent of the cellular wall of the fruiting body or of the mycelium. the aims of this work were to select a culture medium that maximized the production of biomass and polysaccharides produced by pleurotus ostreatus dsm 1833 and to evaluate the selected medium in two values of initial oxygen transfer rate -k(l)a (10.2 and 19.3 h(-1)). a 2* *4 factorial design was constructed to evaluate the ...200817306976
oyster mushroom reduced blood glucose and cholesterol in diabetic subjects.it has been postulated that mushroom has beneficial effect of lowering blood glucose and cholesterol in diabetic subjects. the literature so far searched and found that there was no published data in this regard. this study was undertaken to assess the effect of reducing blood glucose, cholesterol and triglycerides in diabetic patients. additionally, this study addressed whether there was any hepatic and renal toxicity of mushroom. this clinical investigation was conducted in birdem hospital fro ...200717344789
ostreolysin affects rat aorta ring tension and endothelial cell viability in vitro.the effects of ostreolysin, a cardiotoxic cytolysin produced by the edible oyster mushroom (pleurotus ostreatus), were studied on tension development in isolated rat aortic ring. its cytotoxic effects on human umbilical vein endothelial cells and chinese hamster lung fibroblasts were also studied. ostreolysin induced a concentration-dependent increase in aortic ring tension in the range from 5 to 30 microg/ml, and was cytotoxic to both cell lines. these effects could contribute significantly to ...200717350066
lentinus edodes and pleurotus species lignocellulolytic enzymes activity in submerged and solid-state fermentation of lignocellulosic wastes of different composition.lentinus edodes and pleurotus species from various origins were compared for the first time for their ability to produce lignocellulolytic enzyme in solid-state (ssf) and submerged (sf) fermentation of various plant raw material. fungi cultivation in identical culture conditions revealed wide differences among both species and strains of the same species. the yields of cmcase (62.3uml(-1)), xylanase (84.1 u ml(-1)), fpa (5.9 u ml(-1)), and laccase (4103 ul(-1)) are the best so far obtained with ...200817350827
effect of ostreolysin, an asp-hemolysin isoform, on human chondrocytes and osteoblasts, and possible role of asp-hemolysin in pathogenesis.aspergillus fumigatus, a pathogenic mould causing a wide range of diseases including aspergillosis, produces a series of toxic substances which appear to act in an additive and/or synergic way on cells. aspergillosis generally occurs in immunocompromised hosts or is associated with organ transplantation. from the muscul skeleton system the vertebrae, ribs and orbit are the most commonly affected, while the joints are less frequent targets. the cytolytic protein asp-hemolysin is one of the toxins ...200717365648
structural characterization of heterodimeric laccases from pleurotus ostreatus.the subfamily of poxa3 laccase isoenzymes produced by the fungus pleurotus ostreatus has been characterized as an example of the complexity and heterogeneity of fungal isoenzyme patterns. two isoenzymes, poxa3a and poxa3b, were previously purified, exhibiting an unusual heterodimeric structure composed of a large (67 kda) and a small (18 or 16 kda) subunit. a unique gene encodes the large subunit of both poxa3a and poxa3b, but alternative splicing produces two variants--differing for an insertio ...200717429621
effects of hydrophobic amino acid substitution in pleurotus ostreatus proteinase a inhibitor 1 on its structure and functions as protease inhibitor and intramolecular chaperone.we previously demonstrated that pleurotus ostreatus proteinase a inhibitor 1 (poia1) could function as an intramolecular chaperone of subtilisin bpn', as in the case of the propeptide of subtilisin bpn', and that its phe44 --> ala mutant, which lost its tertiary structure, could not assist the refolding of subtilisin bpn'. in this study, we examined the effects of hydrophobic amino acid substitutions at other sites and substitutions of phe44 with other hydrophobic residues on the structure and f ...200717437962
production of lignocellulose-degrading enzymes and changes in soil bacterial communities during the growth of pleurotus ostreatus in soil with different carbon content.the extracellular enzyme activity and changes in soil bacterial community during the growth of the ligninolytic fungus pleurotus ostreatus were determined in nonsterile soil with low and high available carbon content. in soil with p. ostreatus, the activity of ligninolytic enzymes laccase and mn-peroxidase was several orders of magnitude higher than in soil without the fungus. addition of lignocellulose to soil increased the activity of cellulolytic fungi and the production of mn-peroxidase by p ...200617455795
the protective effect of selenium inorganic forms against cadmium and silver toxicity in mycelia of pleurotus ostreatus.the effect of two inorganic selenium forms has been investigated in the mycelia of pleurotus ostreatus exposed to cadmium and silver salts in the shaken cultures. the degree of toxicity was assessed by the determination of malondialdehyde (mda; a common biomarker of lipid peroxidation). the mycelia were exposed to one element form (up to 5 mg l(-1)) and also to the following combinations: cadmium(ii) + selenium(iv); cadmium(ii) + selenium(vi); silver(i) + selenium(iv); silver(i) + selenium(vi). ...200717509851
interspecific interactions with trichoderma longibrachiatum induce pleurotus ostreatus defence reactions based on the production of laccase isozymes.laccases are phenoloxidases involved in aromatic compound transformation but also in stress response towards antagonist species such as trichoderma sp. in this study intracellular isoforms of laccases produced by pleurotus ostreatus in liquid cultures with or without trichoderma longibrachiatum showed five isoforms with various intensities depending on the culture conditions suggesting a basal expression of these enzymes, which can be induced by interspecific interactions. a first attempt to ana ...200717609858
characterization of a pleurotus ostreatus fruiting body-specific hydrophobin gene, po.hyd.hydrophobins are a family of small, moderately hydrophobic proteins with eight cysteine residues arranged in a conserved pattern. a full-length cdna, designated po.hyd, corresponding to a hydrophobin gene of pleurotus ostreatus was obtained in our previous work. the po.hyd gene contains a 333 bp open reading frame (orf), which is interrupted by two typical classi introns. there was no consensus signal for a polya tail detected in the 3'untranslated region. however, an analogous t- or tg-rich mot ...200717647210
broiler litter supplementation improves storage and feed-nutritional value of sawdust-based spent mushroom substrate.a study was conducted to determine the effect of broiler poultry litter (bl) supplementation to spent mushroom substrate (sms) on its storage and feed-nutritional value improvement. in exp. 1, the sawdust-based sms from a king oyster mushroom (pleurotus eryngii) farm was mixed with bl at 0%, 25%, 50%, 75% and 100% levels on a wet basis and deepstack stored for short-term (1-wk) and long-term (3-wk). at 1-wk of short-term deepstack storage, sms with more than 50% bl levels showed favorable conser ...200817698348
carnivorous mushrooms.ten species of gilled fungi, including the oyster mushroom (pleurotus ostreatus), have been shown to attack and consume nematodes. it is suggested that these wood-decay fungi utilize the nutrients in their prey to supplement the low levels of nitrogen available in wood. this mode of nutrition is similar in principle to that of carnivorous higher plants.198417783527
genetically closely related but phenotypically divergent trichoderma species cause green mold disease in oyster mushroom farms worldwide.the worldwide commercial production of the oyster mushroom pleurotus ostreatus is currently threatened by massive attacks of green mold disease. using an integrated approach to species recognition comprising analyses of morphological and physiological characters and application of the genealogical concordance of multiple phylogenetic markers (internal transcribed spacer 1 [its1] and its2 sequences; partial sequences of tef1 and chi18-5), we determined that the causal agents of this disease were ...200717827333
thermal requirement for development of sancassania rodionovi (acari: acaridae) on mushrooms.the free-living mite species sancassania rodionovi (zachvatkin) (acari: acaridae), is a serious pest of mushrooms in iran. studies were conducted to examine the development of this mite in relation to temperature on two mushroom species: agaricus bisporus lange (button mushroom) and pleurotus ostreatus kummer (oyster mushroom). the developmental time of this acarid mite was studied at eight constant temperatures, ranging from 5 to 40 degrees c, and developmental rates were modeled as a function ...200717849857
assessment of selected antioxidants in tomato pomace subsequent to treatment with the edible oyster mushroom, pleurotus ostreatus, under solid-state fermentation.tomato pomace, delignified by the edible oyster mushroom, pleurotus ostreatus, could be used as a poultry feed ingredient to provide alpha-tocopherol for retardation of lipid oxidation in postmortem meat if the antioxidant were retained in pomace after fungal fermentation. experiments were conducted to investigate changes in the content of alpha-tocopherol, lycopene, and beta-carotene in tomato pomace after sterilization and treatment with p. ostreatus from 0 to 104 days. alpha-tocopherol (39.26 ...200717902625
wheat bran biodegradation by pleurotus ostreatus: a solid-state carbon-13 nmr study.solid-state (13)c nuclear magnetic resonance (nmr) and elemental analysis techniques were used to monitor the degradation of wheat bran by the white-rot fungus pleurotus ostreatus during a 62-day cultivation period. the weight loss and in vitro organic matter digestibility of the substrate were also evaluated after fungal treatment. the (13)c nmr spectra of degraded wheat bran samples showed a lower content in carbohydrates and a higher content in aliphatic and carboxylic groups than the untreat ...200817920878
isolation of genes expressed during the developmental stages of the oyster mushroom, pleurotus ostreatus, using expressed sequence tags.in order to isolate and identify the developmentally regulated genes during fruiting body development, cdna libraries were constructed from eight developmental stages of the oyster mushroom, pleurotus ostreatus. from these libraries, 11 761 expressed sequence tags (poests) were generated. of these, 4060 different genes (pounigenes) were identified, representing 34.5% of the entire genome. redundancy analysis of ests during the developmental stages identified eight, 13 and two genes that were spe ...200717937661
isolation of dna topoisomerase ii gene from pleurotus ostreatus and its application in phylogenetic analysis.we performed experiments to isolate dna topoisomerase ii gene from pleurotus ostereatus and to discuss its application as a feasible and credible molecular maker in phylogenitic analysis.200717953613
decolourisation of mushroom farm wastewater by pleurotus ostreatus.mushroom production on coffee pulp as substrate generates an intense black residual liquid, which requires suitable treatment. in the present study, pleurotus ostreatus growth in wastewater from mushroom farm was evaluated as a potential biological treatment process for decolourisation as well as to obtain biomass (liquid inoculum). culture medium components affecting mycelial growth were determined, evaluating colour removal. laccase activity was monitored during the process. p. ostreatus was a ...200817957486
fatty acid profiles of polar and non-polar lipids of pleurotus ostreatus and p. cornucopiae var. 'citrino-pileatus' grown at different temperatures.the application of fatty acid (fa) composition data has now extended to studies of physiology, chemotaxonomy, and intrageneric differentiation, as well as to studies of human nutrition. environmental factors such as nutritional components, oxygen, and temperature are known to affect lipid content and composition in living organisms, including fungi. in the present study, the polar and non-polar lipid content of pleurotus ostreatus and p. cornucopiae var. citrino-pileatus fruiting bodies produced ...200717988848
ostreolysin enhances fruiting initiation in the oyster mushroom (pleurotus ostreatus).fruiting initiation in mushrooms can be triggered by a variety of environmental and biochemical stimuli, including substances of natural or synthetic origin. in this work ostreolysin, a cytolytic protein specifically expressed during the formation of primordia and fruit bodies of pleurotus ostreatus, was applied to nutrient media inoculated with mycelium of p. ostreatus, and its effects on mycelial growth and fructification of the mushroom studied. the addition of ostreolysin slightly inhibited ...200718037282
heterologous expression of heterodimeric laccase from pleurotus ostreatus in kluyveromyces lactis.among the laccases produced by the white-rot fungus pleurotus ostreatus, there are two closely related atypical isoenzymes, poxa3a and poxa3b. these isoenzymes are endowed with quaternary structure, consisting of two subunits very different in size. the poxa3 large subunit is clearly homologous to other known laccases, while the small subunit does not show significant homology with any protein in data banks. to investigate on the singular structure of the poxa3 complex, a new system for recombin ...200818043917
genetic variability and phylogenetic relationship among proton-beam-irradiated strains of pleurotus ostreatus.to assess the effects of a proton beam on oyster mushrooms (pleurotus ostreatus), the genetic diversity and phylogenetic relationships among strains induced by a proton beam were investigated based on a clustering analysis. according to an aflp dna polymorphism analysis, the induced strains were divided into four groups that coincided with the dose. when applying proton-beam radiation, the dissimilarity among the induced strains increased when increasing the dose. when using more than 400 gy, th ...200718050925
purification, characterization, and cloning of fibrinolytic metalloprotease from pleurotus ostreatus mycelia.a fibrinolytic protease (pofe) was purified from the cultured mycelia of the edible oyster mushroom pleurotus ostreatus, using a combination of various chromatographies. the purification protocol resulted in an 876-fold purification of the enzyme, with a final yield of 6.5%. the apparent molecular mass of the purified enzyme was estimated to be 32 kda by sds-page, fibrin-zymography, and size exclusion using fplc. the optimal reaction ph value and temperature were ph 6.5 and 35 degrees c, respect ...200718051595
decolorization of cotton pulp black liquor by pleurotus ostreatus in a bubble-column reactor.decolorization of cotton pulp black liquor by pleurotus ostreatus b1 in a bubble-column reactor (bcr) was studied. the optimal conditions for the running of bcr are 30 degrees c, ph 6.0, aeration rate 1.2 l min(-1), and mycelial age 7 days. under the optimal conditions, the bcr was run for four cycles (each cycle, 12 days) and the same mycelial pellets were reused. the ultimate decolorization and cod removal rates are 76% and 80%, respectively.200818058052
detection of the mycovirus omsv in the edible mushroom, pleurotus ostreatus, using an spr biosensor chip.a surface plasmon resonance (spr) biosensor chip was developed for the rapid detection of the oyster mushroom spherical virus (omsv), which causes a mushroom die-back disease, the symptoms of which include malformed fruiting bodies and retarded mycelial growth in the cultivated edible mushroom, pleurotus ostreatus. an anti-omsv monoclonal antibody (mab) was generated initially using purified omsv viral particles. for the fabrication of the biosensor chip, the anti-omsv mab was layered onto an ac ...200818079008
optimizing edible fungal growth and biodegradation of inedible crop residues using various cropping methods.long-term manned space flights to mars require the development of an advanced life support (als) ecosystem including efficient food crop production, processing and recycling waste products thereof. using edible white rot fungi (ewrf) to achieve effective biomass transformation in als requires optimal and rapid biodegradative activity on lignocellulosic wastes. we investigated the mycelial growth of lentinula edodes and pleurotus ostreatus on processed residues of various crops under various crop ...200818155518
inter-subspecies hybrid dikaryons of oyster mushroom independently isolated in vietnam and japan.two strains of the mushroom pleurotus, isolated from nature in vietnam and japan, contained a similar combination of two distinct rdna internal transcribed spacer (its) sequences. they were perhaps hybrid dikaryons between p. cystidiosus subsp. abalonus and a novel p. cystidiosus subspecies. these mushrooms produce dikaryotic arthroconidia. this unique asexual reproduction might allow stable maintenance of a particular pair of nuclei.200818175897
development of new laccases by directed evolution: functional and computational analyses.laccases are blue multicopper oxidases that couple the four-electron reduction of oxygen with the oxidation of a broad range of aromatic substrates. these fungal enzymes can be used for many applications such as bleaching, organic synthesis, bioremediation, and in laundry detergents. laccases from pleurotus ostreatus have been successfully heterologously expressed in yeasts. the availability of established recombinant expression systems has allowed the construction of mutated, "better performing ...200818186469
an application of protein microarray in the screening of monoclonal antibodies against the oyster mushroom spherical virus.the oyster mushroom spherical virus (omsv) is a causative agent of dieback disease in the oyster mushroom, pleurotus ostreatus. outbreaks of this virus occasionally result in serious disease that is associated with hefty economic losses. thus, the detection and removal of omsv-infected spawn is considered to be a crucial step for the stable production of p. ostreatus. for the detection of omsv, we attempted to generate monoclonal antibodies (mabs) against an rna polymerase domain (rpd) of an oms ...200818191463
a labeling study on the formation of perillene by submerged cultured oyster mushroom, pleurotus ostreatus.the conversion of beta-myrcene to the furanoid flavour compound perillene by pleurotus ostreatus was investigated using trideutero beta-myrcene, trideutero alpha-(z)-acaridiol and non-labeled 1,2- and 3,10-epoxy-beta-myrcene, alpha,alpha-acarilactol, and perillene as substrates. myrcene diols were formed from the cleavage of myrcene epoxides, but only alpha-(z)-acaridiol, a 1,4-butanediol derivative most likely generated through a base-catalysed epoxide opening, was a suitable precursor of peril ...200818196236
the mitochondrial genome of the basidiomycete fungus pleurotus ostreatus (oyster mushroom).in this study, the full mitochondrial genome of a basidiomycete fungus, pleurotus ostreatus, was sequenced and analyzed. it is a circular dna molecule of 73 242 bp and contains 44 known genes encoding 18 proteins and 26 rna genes. the protein-coding genes include 14 common mitochondrial genes, one ribosomal small subunit protein 3 gene, one rna polymerase gene and two dna polymerase genes. in addition, one rna and one dna polymerase genes were identified in a mitochondrial plasmid. these two gen ...200818248422
psittacine circovirus infection in parakeets of the genus eunymphicus and treatment with beta-(1,3/1,6)-d-glucan.eight captive-bred horned parakeets (eunymphicus cornutus) and four captive-bred major mitchell cockatoos (cacatua leadbeateri) from the same aviary tested positive for psittacine circovirus (pscv) dna in whole blood by nested-polymerase chain reaction (pcr). the chronic form of disease with feather fragility and loss was observed in three horned parakeets. infection in other individuals was subclinical. immunosuppression, either hematologically or as susceptibility to secondary infections, was ...200718251415
role of bacillus spp. in antagonism between pleurotus ostreatus and trichoderma harzianum in heat-treated wheat-straw substrates.this study aimed to identify bacteria involved in trichodermaharzianum inhibition while promoting pleurotus ostreatus defences in order to favour cultivation-substrate selectivity for mushroom production. pcr-dgge profiles of total dna from wheat-straw substrate showed weak differences between bacterial communities from substrate inoculated with p. ostreatus with or without t. harzianum. the major cultivable bacteria were isolated from three batches of wheat-straw-based cultivation substrates sh ...200818295481
temperature affects the production, activity and stability of ligninolytic enzymes in pleurotus ostreatus and trametes versicolor.enzyme activity was determined in cultures of pleurotus ostreatus and trametes versicolor with cellulose as a sole c source and high c/n ratio. the fungi were able to grow and produce laccase and mn-peroxidase (mnp) at 5-35 degrees c, the highest production being recorded at 25-30 degrees c in p. ostreatus and at 35 degrees c in t. versicolor. production of both enzymes at 10 degrees c accounted only for 4-20% of the maximum value. temperature optima for enzyme activity were 50 and 55 degrees c ...200718298047
mechanism for oxidation of high-molecular-weight substrates by a fungal versatile peroxidase, mnp2.unlike general peroxidases, pleurotus ostreatus mnp2 was reported to have a unique property of direct oxidization of high-molecular-weight compounds, such as poly r-478 and rnase a. to elucidate the mechanism for oxidation of polymeric substrates by mnp2, a series of mutant enzymes were produced by using a homologous gene expression system, and their reactivities were characterized. a mutant enzyme with an ala substituting for an exposing trp (w170a) drastically lost oxidation activity for verat ...200818326680
toxicity of plant essential oils and their components against lycoriella ingenua (diptera: sciaridae).plant essential oils from 20 plant species were tested for their insecticidal activity against larvae of lycoriella ingenua (dufour) (diptera: sciaridae) by using a fumigation bioassay. good insecticidal activity (>90%) against larvae of l. ingenua was achieved with essential oils of caraway seed carum carvi (l.)], lemongrass [cymbopogon citratus (d.c.) stapf.], mandarine (citrus reticulate blanco), nutmeg (myristica fragrans houtt), cade (juniperus oxycedrus l.), spearmint (mentha spicata l.), ...200818330128
the identification of a novel pleurotus ostreatus dsrna virus and determination of the distribution of viruses in mushroom spores.double-stranded rnas and virus particles were identified in pleurotus ostreatus strain shin-nong in korea. isometric virus particles with a diameter of 33 nm were purified, which are similar to other pleurotus viruses reported previously. this strain contains 5 dsrnas, 8.0, 2.5, 2.4, 2.0, and 1.8 kb in size. the virus particles contain 2 dsrnas, designated rna-1 (2.5 kb), and rna-2 (2.4 kb) which is a typical pattern of partitiviridae. a non-encapsidated dsrna of about 8.0 kb also was identified ...200818337700
[use of kenaf fibre in the elaboration of specific substrates for pleurotus ostreatus (jacq. ex fr.) kummer cultivation].in this study, the viability of the kenaf fibre use, alone or combined with cereal straw, vine shoots and olive mill dried waste, in the elaboration of specific substrates for the cultivation of pleurotus ostreatus (jacq. ex fr.) kummer, second mushroom in importance cultivated in spain, is described. furthermore, three different methods of preparation of the substrate have been considered in order to obtain selectivity for the growth and later fruiting of pleurotus sporophore. as for the produc ...200818338932
a study of mechanisms responsible for incorporation of cesium and radiocesium into fruitbodies of king oyster mushroom (pleurotus eryngii).ex vitro cultures of pleurotus eryngii were carried out under controlled conditions using sterile medium composed of barley seeds. the influence of alkali and alkaline earth element salts (cscl, kcl, nacl, rbcl, and cacl(2)) and tetraethylammonium chloride on incorporation of cesium, potassium, sodium, rubidium and calcium, and their distribution within fruitbodies, was examined. the results show that incorporation of cesium into fruitbodies was not suppressed by na(+) and rb(+) or tetraethylamm ...200818342998
effect of substrate particle size and additional nitrogen source on production of lignocellulolytic enzymes by pleurotus ostreatus strains.two strains of pleurotus ostreatus (ie-8 and cp-50) were grown on defined medium added with wheat straw extract (wse). mycelia from these cultures were used as an inoculum for solid fermentation using sugar cane bagasse (c:n=142). this substrate was used separately either as a mixture of heterogeneous particle sizes (average size 2.9 mm) or as batches with two different particle sizes (0.92 mm and 1.68 mm). protein enrichment and production of lignocellulolytic enzymes on each particle size was ...200818359224
the toxin produced by pleurotus ostreatus reduces the head size of nematodes.many detrimental nematodes exist, including parasitic plant and animal nematodes. the pleurotus ostreatus (oyster mushroom) is a famous mushroom that preys upon live nematodes. however, there have been no details reported on the mechanism of this predatory activity. therefore, we investigated the predatory relationship between the nematode and p. ostreatus as a potential way of exterminating other various detrimental nematodes. upon invasion by the nematode, the mushroom defends itself by causin ...200818379043
fungal bioremediation of creosote-treated wood: a laboratory scale study on creosote components degradation by pleurotus ostreatus mycelium.a bioremediation system for creosote-treated wood is proposed, based on the detoxifying capability of pleurotus ostreatus, a ligninolythic fungus. non-sterilized chipped contaminated wood was mixed at various ratios with wheat straw on which pleurotus mycelia was grown. at 1:2 initial ratio contaminated wood:wheat straw, chemical analyses demonstrated an almost complete degradation of creosote oil components after 44 days, also confirmed by a significant reduction of ecotoxicity. lower ratios, i ...200818389164
combined action of a bacterial monooxygenase and a fungal laccase for the biodegradation of mono- and poly-aromatic hydrocarbons.the combined action of a wide substrate range toluene o-xylene monooxygenase from pseudomonas sp. ox1, able to convert many aromatic compounds into mono- and di-hydroxylated derivatives, and fungal laccases from pleurotus ostreatus which oxidize these hydroxylated products yielding polymers with reduced toxicity is described. this strategy permits to overcome many of the substrate specificity problems and dead end toxic products formation generally encountered in complex bacterial biodegradation ...200818407494
in situ recovery of the aroma compound perillene from stirred-tank cultured pleurotus ostreatus using gas stripping and adsorption on polystyrene.supplementation of the key metabolite, alpha-(z)-acaridiol, to stirred-tank cultured pleurotus ostreatus was used to demonstrate that integrated in situ product recovery resulted in high conversion rates and quantitative separation of the target product perillene from the nutrient medium. the conversion of beta-myrcene by p. ostreatus was scaled-up from shake-flasks into a controlled, stirred tank bioreactor equipped with gas stripping and adsorption on a polystyrene fixed bed. the formation of ...200818414807
evaluation of two wild types of pleurotus ostreatus (mcc07 and mcc20) isolated from nature for their ability to decolorize benazol black zn textile dye in comparison to some commercial types of white rot fungi: pleurotus ostreatus, pleurotus djamor, and pleurotus citrinopileatus.biological decolorization of benazol black zn, a reactive azo-type textile dyestuff, was comparatively studied using 3 different commercial-type white rot fungi strains (pleurotus ostreatus, pleurotus cornucopiae var. citrinopileatus, pleurotus djamor, and 2 wild types of p. ostreatus (mcc07 and mcc20) isolated from the nature. the initial dye concentrations in the medium were 500 and 1000 mg.l-1. all the organisms studied decolorized benazol black zn to varying degrees. at low dye concentration ...200818449221
lysophospholipids prevent binding of a cytolytic protein ostreolysin to cholesterol-enriched membrane domains.ostreolysin, a 15kda pore-forming protein from the oyster mushroom (pleurotus ostreatus), binds specifically to cholesterol-enriched membrane domains existing in the liquid-ordered phase, and lyses cells and lipid vesicles made of cholesterol and sphingomyelin. we have monitored binding of sub-lytic concentrations of ostreolysin to membranes of chinese hamster ovary cells and rat somatotrophs, using primary anti-ostreolysin and fluorescence-labeled secondary antibodies detected by confocal micro ...200818455213
prediction of biodegradation rate constants of hydroxylated polychlorinated biphenyls by fungal laccases from trametes versicolor and pleurotus ostreatus.quantitative structure-activity relationship (qsar) models for fungal laccase-catalyzed degradation of different hydroxylated polychlorinated biphenyls (oh-pcbs) were developed using some fundamental quantum chemical descriptors. the cross-validated q(2)(cum )values for the two optimal qsar models are as high as 0.958 and 0.961 for laccases from trametes versicolor and pleurotus ostreatus, respectively, indicating good predictive abilities for laccase-catalyzed degradation of oh-pcbs. results fr ...200818461267
biodegradation of endocrine-disrupting phthalates by pleurotus ostreatus.biodegradation of endocrine-disrupting phthalates [diethyl phthalate (dep), dimethyl phthalate (dmp), butylbenzyl phthalate (bbp)] was investigated with 10 white rot fungi isolated in korea. when the fungal mycelia were added together with 100 mg/l of phthalate into yeast extract-malt extract-glucose (ymg) medium, pleurotus ostreatus, irpex lacteus, polyporus brumalis, merulius tremellosus, trametes versicolor, and t. versicolor mrp1 and mrp13 (transformant of the mn-repressed peroxidase gene of ...200818467874
[production of biomass and ligninolytic enzymes by pleurotus ostreatus in submerged culture.].the production of biomass and ligninolytic enzymes by pleurotus ostreatus was analysed in synthetic medium with yeast extract and different glucose concentrations (0.5 - 20 g/l), at different ph (3.5-6.5) and incubation temperatures (23-32 degrees c). the best culture condition were: initial glucose concentration of 5 g/l, initial ph between 5.5-6.5 and incubation temperature between 26-29 degrees c. the saturation constant for glucose (ks) was 1.75 g/l. the biomass concentration reached 8.6 g/l ...199818473524
decolorization and detoxication of reactive industrial dyes by immobilized fungi trametes pubescens and pleurotus ostreatus.trametes pubescens and pleurotus ostreatus, immobilized on polyurethane foam cubes in bioreactors, were used to decolorize three industrial and model dyes at concentrations of 200, 1000 and 2000 ppm. five sequential cycles were run for each dye and fungus. the activity of laccase, mn-dependent and independent peroxidases, lignin peroxidase, and aryl-alcohol oxidase were daily monitored during the cycles and the toxicity of media containing 1000 and 2000 ppm of each dye was assessed by the lemna ...200818481217
[bioremediation of oil-polluted soil with an association including the fungus pleurotus ostreatus and soil microflora].the possibility of application of the pleurotus ostreatus d1-soil microflora to bioremediation of oil-polluted soils was studied. the fungus degraded mainly the aromatic fraction, whereas soil microflora intensely degraded paraffin and naphthene oil fractions. introduction of the fungus pleurotus ostreatus d to soil induces degradation of a wider range of oil hydrocarbons. it is reasonable to further investigate the discovered phenomenon in order to improve procedures of remediation of oil-pollu ...201618491600
mycelium growth and degradation of creosote-treated wood by basydiomycetes.tolerance of wood decay fungi of the genera agrocybe, armillaria, auricularia, daedalea, pleurotus, trametes to the presence of various amounts of creosote-treated wood (ctw) in the growth medium was compared. in the case of the most tolerant strain, pleurotus ostreatus smr 684, extracellular laccase and peroxidase specific activities were monitored during growth in the presence of ctw. degradation of various creosote-constituting polycyclic aromatic hydrocarbons by this strain was evaluated by ...200818501950
immunomodulatory capacity of fungal proteins on the cytokine production of human peripheral blood mononuclear cells.immunomodulation by fungal compounds can be determined by the capacity of the compounds to influence the cytokine production by human peripheral blood mononuclear cells (hpbmc). these activities include mitogenicity, stimulation and activation of immune effector cells. eight mushroom strains (agaricus blazei, coprinus comatus, flammulina velutipes, ganoderma lucidum, grifola frondosa, volvariella volvacea, lentinus edodes, and pleurotus ostreatus) were tested for the immunomodulating activity of ...200818550017
heat and microbial treatments for nutritional upgrading of wheat straw.the ligninolytic activities of four cellulolytic organisms were compared using straw. only aspergillus japonicus and polyporous versicolor appreciably degraded lignin with a. japonicus yielding the most protein. in solid culture, most protein was produced by p. versicolor, closely followed by a. japonicus. pretreatment of the straw by hot water facilitated biodegradation and protein production. the nutritional value of the residual straw was also increased by some fungal cultures. the greatest a ...198618555339
biodegradation of fluoranthene by basidiomycetes fungal isolate pleurotus ostreatus hp-1.the biodegradation of fluoranthene, a high molecular weight polycyclic aromatic hydrocarbon (pah), was investigated in submerged culture using the wood decaying fungus isolated from forest locality in gujarat, india. the basidiomycete fungal isolate was found to have an ability to grow on sabaroud dextrose agar containing 50 mgl(-1) of each naphthalene, anthracene, acenaphthene, benzo (a) anthracene, pyrene, flouranthene, carbazole, and biphenyl. the involvement of extracellular fungal peroxidas ...200918574565
dearomatization of lignin derivatives by fungal protocatechuate 3,4-dioxygenase immobilized on porosity glass.protocatechuate 3,4-dioxygenase (see protocatechuate: oxygen 3,4-oxidoreductase, ec 1.13.11.3) was isolated from the mycelium of pleurotus ostreatus (induced with p-hydroxybenzoic acid) and immobilized on controlled porosity glass beads. four fractions of na-lignosulfonates (varying in m(r), after chromatography on sephadex g-50) were treated with the immobilized enzyme. the products after incubation showed the same m(r) as the untreated fractions, but their light absorption at 280 nm considerab ...198818587748
lignocellulolytic enzymes profile during growth and fruiting of pleurotus ostreatus on wheat straw and tree leaves.cultivation of two commercial pleurotus ostreatus (oyster mushroom) strains was performed in plastic bags. tree leaves appeared to be an excellent growth substrate for the conversion into fruiting bodies with biological efficiency of 108-118%. the level of enzyme activity was strongly regulated during the life cycle of mushrooms. however, despite the quantitative variations, each strain had a similar pattern of enzyme accumulation in fermentation of both substrates. laccase and mnp activities we ...200818595320
purification, structure and immunobiological activity of a water-soluble polysaccharide from the fruiting body of pleurotus ostreatus.the water-soluble polysaccharide (pop), with a molecular mass of 2.4x10(4)da, was obtained from the fruiting body of pleurotus ostreatus. structure features of the purified polysaccharide were investigated by a combination of chemical and instrumental analysis, such as methylation analysis, smith degradation, gc-ms, (13)c and (1)h nmr and ftir. the results indicated that the backbone of pop was composed of (1-->6)-linked-alpha-d-galactopyranosyl and (1-->2,6)-linked-alpha-d-galactopyranosyl resi ...200918667302
induction of fruiting in oyster mushroom (pleurotus ostreatus) by polymeric 3-alkylpyridinium salts.polymeric 3-alkylpyridinium salts (poly-aps), surface-active compounds from the marine sponge reniera sarai, have been shown to stimulate the fruit body formation from pleurotus ostreatus mycelium. in nutrient media supplemented with poly-aps (>or= 0.01 microg ml(-1)), the formation of primordia and development of fruit bodies were detected approximately 10d earlier than in the absence of poly-aps, and also led to a considerably larger quantity of young mushrooms. this effect appears to be speci ...200818692375
laccases of pleurotus ostreatus observed at different phases of its growth in submerged fermentation: production of a novel laccase isoform.the production of laccases during the lag, exponential and stationary phases of growth of pleurotus ostreatus in submerged fermentation was evaluated. laccase activity was positively correlated to the growth of the fungus. the specific growth rate was 0.02 h(-1) and the highest amount of dry biomass (7.8 gl(-1)) was obtained after 480 h of growth. four laccase isoforms were secreted by the fungus, and tentatively named l(i)1, l(i)2, l(i)3 and l(i)4. l(i)2, l(i)3 and l(i)4 were produced during th ...200818692377
clinical studies on the hypolipidemic and antioxidant effects of selected natural substances.the average blood levels of cholesterol and triacylglycerol in population are generally considered to be indicators of cardiovascular risk.200818700439
effect of growth substrate, method of fermentation, and nitrogen source on lignocellulose-degrading enzymes production by white-rot basidiomycetes.the exploration of seven physiologically different white rot fungi potential to produce cellulase, xylanase, laccase, and manganese peroxidase (mnp) showed that the enzyme yield and their ratio in enzyme preparations significantly depends on the fungus species, lignocellulosic growth substrate, and cultivation method. the fruit residues were appropriate growth substrates for the production of hydrolytic enzymes and laccase. the highest endoglucanase (111 u ml(-1)) and xylanase (135 u ml(-1)) act ...200818716810
mobilizing agents enhance fungal degradation of polycyclic aromatic hydrocarbons and affect diversity of indigenous bacteria in soil.the impact of several mobilizing agents (mas) (i.e., soybean oil, tween-20, tween-80, olive-oil mill wastewaters, and randomly methylated beta-cyclodextrins) on the degradation performances of the white-rot fungi irpex lacteus and pleurotus ostreatus was comparatively assessed in a soil spiked with a mixture of seven polycyclic aromatic hydrocarbons (pahs). among the different mas, soybean oil best supported the growth of both fungi that was twice that observed in soil in the absence of mas. in ...200818727031
manganese amendment and pleurotus ostreatus treatment to convert tomato pomace for inclusion in poultry feed.if freshly dried tomato pomace could be fed to poultry, its naturally occurring alpha-tocopherol would retard lipid oxidation during further processing, long-term frozen storage, and heating of poultry meat; however, the high fiber content in this agricultural by-product adversely affects its use. experiments were conducted to investigate the chemical composition and in vitro true digestibility of amended (without and with 487 micromol of manganese/g) tomato pomace substrate after treatment with ...200818753459
bio-remediation of colored industrial wastewaters by the white-rot fungi phanerochaete chrysosporium and pleurotus ostreatus and their enzymes.the effect of phanerochaete chrysosporium and pleurotus ostreatus whole cells and their ligninolytic enzymes on models of colored industrial wastewaters was evaluated. models of acid, direct and reactive dye wastewaters from textile industry have been defined on the basis of discharged amounts, economic relevance and representativeness of chemical structures of the contained dyes. phanerochaete chrysosporium provided an effective decolourization of direct dye wastewater model, reaching about 45% ...200918758969
combinations of mild physical or chemical pretreatment with biological pretreatment for enzymatic hydrolysis of rice hull.two novel two-step pretreatments for enzymatic hydrolysis of rice hull (rh) were proposed to lower the severity requirement of fungal pretreatment time. they consisted of a mild physical or chemical step (ultrasonic and h(2)o(2)) and a subsequent biological treatment (pleurotus ostreatus). the combined pretreatments led to significant increases of the lignin degradation than those of one step pretreatments. after enzymatic hydrolysis of the pretreated rh, the net yields of total soluble sugar (t ...200918760920
growth and laccase production by pleurotus ostreatus in submerged and solid-state fermentation.pleurotus ostreatus showed atypical laccase production in submerged vs. solid-state fermentation. cultures grown in submerged fermentation produced laccase at 13,000 u l(-1), with a biomass production of 5.6 g l(-1) and four laccase isoforms. however, cultures grown in solid-state fermentation had a much lower laccase activity of 2,430 u l(-1), biomass production of 4.5 g l(-1), and three laccase isoforms. these results show that p. ostreatus performs much better in submerged fermentation than i ...200818762938
study of laccase production by pleurotus ostreatus in a 5 l bioreactor and application of the enzyme to determine the antioxidant concentration of human plasma.to achieve high laccase production from pleurotus ostreatus in a bench top bioreactor and to utilize the enzyme for determination of the total antioxidant concentration (tac) of human plasma.200818778375
determination of the degradation products of selected sulfonated phenylazonaphthol dyes treated by white rot fungus pleurotus ostreatus by capillary electrophoresis coupled with electrospray ionization ion trap mass spectrometry.the removal of water-soluble sulphonated phenylazonaphthol dye effluents generated by textile industries is an important issue in wastewater treatment. microbial treatment of environmental pollutants including dyes, with white rot fungi has received wide attention as a potential alternative for conventional methods in wastewater treatment. three sulphonated phenylazonaphthol dyes with similar molecular structures acid orange 7, acid orange 8 and mordant violet 5 were selected and degraded by the ...200818778834
fungal bioremediation of the creosote-contaminated soil: influence of pleurotus ostreatus and irpex lacteus on polycyclic aromatic hydrocarbons removal and soil microbial community composition in the laboratory-scale study.the aim of this study was to determine the efficacy of selected basidiomycetes in the removing of polycyclic aromatic hydrocarbons (pah) from the creosote-contaminated soil. fungi pleurotus ostreatus and irpex lacteus were supplemented with creosote-contaminated (50-200mgkg(-1) pah) soil originating from a wood-preserving plant and incubated at 15°c for 120d. either fungus degraded pah with 4-6 aromatic rings more efficiently than the microbial community present initially in the soil. pah remova ...200818782639
[determination of pleurotus ostreatus, pleurotus pulmonarius and paxillus involutus toxicity over artemia salina].we report the toxicity of ethanolic extracts in pleurotus ostreatus, pleurotus pulmonarius and paxillus involutus over that obtained in artemia salina. p. involutus showed the highest toxicity (lc50 = 94.4 microg/ml), similar to that detected using potassium dichromate pattern. p. pulmonarius and p. ostreatus did not show toxicity over a. salina in concentrations lower than 1,000 microg/ml.200818785792
pleurotus ostreatus, an oyster mushroom, decreases the oxidative stress induced by carbon tetrachloride in rat kidneys, heart and brain.the present work is aimed at evaluating the protective effect of the oyster mushroom, pleurotus ostreatus on carbon tetrachloride (ccl4)-induced toxicity in male wistar rats. significantly elevated mean levels (p<0.05) of malondialdehyde (mda) and lowered mean levels (p<0.01) of reduced glutathione (gsh), vitamins c and e (p<0.05) were observed in kidneys, heart and brain of rats exposed to ccl4, when compared to values in normal rats. quantitative and qualitative analysis of catalase (cat), sup ...200818786523
the use of spent brewery grains for pleurotus ostreatus cultivation and enzyme production.in the brewing industry, spent brewery grains (sbgs) are byproducts with a low economic value. the potential use of this leftover as a substrate ingredient for pleurotus ostreatus fruiting body cultivation and enzyme production was evaluated. the best substrate mixture for p. ostreatus mycelium growth comprised 30% wheat bran (wb), 68% beech sawdust (bs) and 2% caco3. on the substrates containing sbg, the fastest mycelium growth was observed on the substrate composed of 10% sbg, 20% wb, 68% bs a ...201518789406
a gc-ms study of the volatile organic composition of straw and oyster mushrooms during maturity and its relation to antioxidant activity.mushrooms are very popular in the market for their nutritional and medicinal use. mushroom volatiles are not only an important factor in the flavor, but also contain many antioxidant compounds. antioxidant activity is a very important property for disease prevention. the volatile compositional characteristics of straw mushrooms (volvariella volvacea [bull. ex fr.] sing.) and oyster mushrooms (pleurotus ostreatus [jacq. ex fr.] kummer) during maturity and the mushroom antioxidant activity related ...200818796224
accumulation and localization of cesium in edible mushroom (pleurotus ostreatus) mycelia.the characteristics of cs accumulation and localization in edible mushrooms were examined using the mycelia of pleurotus ostreatus-y1. scanning electron microscope images revealed the existence of white spots, and energy dispersive x-ray microanalyzer analysis indicated the presence of larger amounts of cs and p in these spots in mycelia cultured on medium containing 25 mm cscl. the (137)cs activities in the mycelia were approximately 4-6 times higher than those in water used for (137)cs elution ...200818800803
antimicrobial and antineoplasic activity of pleurotus ostreatus.the objectives of this work were to evaluate the antimicrobial and antineoplasic activity of pleurotus ostreatus dsm 1833. to study the antimicrobial activity, the following extracts were prepared: water infusion of the fresh fruiting bodies (b1), dehydrated fruiting bodies (b2), fresh mycelium (m1), and dehydrated mycelium (m2). polysaccharides from the fresh mycelium were also obtained by water infusion followed by ethanol treatment (ep). the extracts were tested against candida albicans, esch ...200818830827
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