Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| xylitol production from high xylose concentration: evaluation of the fermentation in bioreactor under different stirring rates. | to investigate the production of xylitol by the yeast candida guilliermondii fti 20037, in a bioreactor, from rice straw hemicellulosic hydrolysate with a high xylose concentration. | 2003 | 12859766 |
| xylitol production from sugarcane bagasse hydrolyzate in fluidized bed reactor. effect of air flowrate. | cells of candida guilliermondii immobilized onto porous glass spheres were cultured batchwise in a fluidized bed bioreactor for xylitol production from sugarcane bagasse hemicellulose hydrolyzate. an aeration rate of only 25 ml/min ensured minimum yields of xylose consumption (0.60) and biomass production (0.14 g(dm)/g(xyl)), as well as maximum xylitol yield (0.54 g(xyt)/g(xyl)) and ratio of immobilized to total cells (0.83). these results suggest that cell metabolism, although slow because of o ... | 2003 | 12892483 |
| a clarification of the relationship of candida guilliermondii to other yeasts by a study of their mating types. | 1954 | 13211564 | |
| [biosynthesis of riboflavin in candida guilliermondii (a. cast) langeron and guerra and some related species; influence of trace elements, especially iron and zinc]. | 1957 | 13522136 | |
| [stimulation of riboflavin synthesis in yeasts candida guilliermondii in the presence of iron in culture medium]. | 1958 | 13632416 | |
| [r-s variation in candida guilliermondii (cast) langerón & guerra]. | 1958 | 13656499 | |
| high-dose liposomal amphotericin b in the therapy of systemic candidiasis in neonates. | high-dose (5-7 mg/kg/day) liposomal amphotericin b was evaluated prospectively during the period 1995-2001 in 41 episodes of systemic candidiasis occurring in 37 neonates (36 of the 37 were premature infants with very low birth weights). median age at the onset of systemic candidiasis was 17 days. candida spp. were isolated from blood in all patients and from urine, skin abscesses and peritoneal fluid in 6, 5 and 1 neonates, respectively. candidiasis was due to candida parapsilosis in 17 cases, ... | 2003 | 13680398 |
| studies on the pathogenesis of experimental candida parapsilosis and candida guilliermondii infections in mice. | 1965 | 14331713 | |
| [effect of iron on the synthesis of riboflavin in respiratory systems of yeasts candida guilliermondii]. | 1959 | 14445623 | |
| interaction of a mixed yeast culture in an "autotroph-heterotroph" system with a closed atmosphere cycle and spatially separated components. | the study considers an experimental model of the "autotroph-heterotroph" system with a closed atmosphere cycle, in which the heterotrophic link is a mixed yeast population. the autotrophic link is represented by the algae chlorella vulgaris and the heterotrophic link by the yeasts candida utilis and candida guilliermondii. the controls are populations of chlorella and the same yeasts isolated from the atmosphere. it has been shown that the outcome of competition in the heterotrophic link depends ... | 2003 | 14503513 |
| [the red mutations impair the regulation of flavinogenesis and metal homeostas in yeast pichia guilliermondii]. | a method of positive selection of mutants with impaired regulation of flavinogenesis and metal homeostasis in yeast pichia guilliermondii was developed. this positive selection system was based on the isolation of pseudo-wild-type revertants (the rib+ phenotype) in riboflavin-dependent rib1-86 mutant (the rib- phenotype) of yeast p. guilliermondii. mutation rib1-86 blocks activity of the gtp cyclohydrolase ii catalyzing the first step in riboflavin (rf) biosynthesis. study of a collection of spo ... | 2003 | 14515457 |
| rapid identification of yeasts commonly found in positive blood cultures by amplification of the internal transcribed spacer regions 1 and 2. | a multiplex pcr method using one universal and eight species-specific primers was developed to rapidly identify eight yeast species found in positive blood cultures. the species-specific primers were designed from the internal transcribed spacer regions 1 and 2 of the rrna gene, whereas the universal primer was located at the 26s rrna gene. the eight species were candida albicans, candida glabrata, candida guilliermondii, candida krusei, candida lusitaniae, candida parapsilosis, candida tropical ... | 2003 | 14557922 |
| species identification of medically important fungi by use of real-time lightcycler pcr. | invasive fungal infection has become a major cause of morbidity and mortality in immunocompromised patients. rapid identification of pathogenic fungi to species level is critical for disease treatment. a real-time lightcycler assay aiming at rapid detection and species identification of pathogenic fungi from clinical isolates was developed. template dnas of different species were amplified and detected in real time by employing sybr green fluorescent dye. the target sequences for species-level d ... | 2003 | 14614065 |
| batch xylitol production by candida guilliermondii fti 20037 from sugarcane bagasse hemicellulosic hydrolyzate at controlled ph values. | batch fermentation of sugarcane bagasse hemicellulosic hydrolyzate by the yeast candida guilliermondii fti 20037 was performed using controlled ph values (3.5, 5.5, 7.5). the maximum values of xylitol volumetric productivity ( q(p)=0.76 g/l h) and xylose volumetric consumption ( q(s)=1.19 g/l h) were attained at ph 5.5. at ph 3.5 and 7.5 the q(p) value decreased by 66 and 72%, respectively. independently of the ph value, y(x/s) decreased with the increase in y(p/s) suggesting that the xylitol bi ... | 2003 | 14624353 |
| batch xylitol production from wheat straw hemicellulosic hydrolysate using candida guilliermondii in a stirred tank reactor. | batch production of xylitol from the hydrolysate of wheat straw hemicellulose using candida guilliermondii was carried out in a stirred tank reactor (agitation speed of 300 rpm, aeration rate of 0.6 vvm and initial cell concentration of 0.5 g l(-1)). after 54 h, xylitol production from 30.5 g xylose l(-1) reached 27.5 g l(-1), resulting in a xylose-to-xylitol bioconversion yield of 0.9 g g(-1) and a productivity of 0.5 g l(-1) h(-1). | 2003 | 14677703 |
| screening and typing of patagonian wine yeasts for glycosidase activities. | the purpose of this study was to select autochthonous glycosidase producer yeasts with potential use in industrial production of patagonian red wines. | 2004 | 14678162 |
| candida guilliermondii fungemia. to treat or not to treat. | 2004 | 14758396 | |
| distribution and antifungal susceptibility of candida species causing candidemia from 1996 to 1999. | susceptibilities to amphotericin b and fluconazole of 383 candida species isolated from blood were determined. candida albicans was the most common species (55.6%), followed by candida parapsilosis (17.5%), candida tropicalis (16.5%), candida glabrata (5.2%), candida guilliermondii (2.3%), and others (2.9%). all but three isolates, candida ciferrii, c. tropicalis, and c. glabrata, one each, were susceptible to amphotericin b. a total of 367 (95.8%) and 15 (4.2%) isolates were susceptible and sus ... | 2004 | 14761719 |
| [multifocal fungal infections in patients after renal transplantation undergoing immunosuppression]. | the aim of the study was to estimate the prevalence of fungi in monofocal and multifocal infections in renal transplant recipients and the characteristics of 60 species and intraspecies features of fungal strains isolated from the organ ontocenoses: oral cavity, rectum and genital organs in 32 patients undergoing permanent immunosuppression after renal transplantation. fungal strains were recovered in 66 out of 96 samples (68.2% off all renal transplant recipients). there were in ontocenoses: or ... | 2003 | 14974373 |
| effect of acetic acid present in bagasse hydrolysate on the activities of xylose reductase and xylitol dehydrogenase in candida guilliermondii. | the first two steps in xylose metabolism are catalyzed by nad(p)h-dependent xylose reductase (xr) (ec 1.1.1.21) and nad(p)-dependent xylitol dehydrogenase (xdh) (ec 1.1.1.9), which lead to xylose-->xylitol-->xylulose conversion. xylitol has high commercial value, due to its sweetening and anticariogenic properties, as well as several clinical applications. the acid hydrolysis of sugarcane bagasse allows the separation of a xylose-rich hemicellulosic fraction that can be used as a substrate for c ... | 2004 | 15107950 |
| structure-activity relationships of derivatives of fusapyrone, an antifungal metabolite of fusarium semitectum. | fusapyrone (1) and deoxyfusapyrone (2) are two 3-substituted-4-hydroxy-6-alkyl-2-pyrones isolated from fusarium semitectum that have considerable antifungal activity against molds. because of their low zootoxicity and selective action they are potentially utilizable along with biocontrol yeasts for control of postharvest crop diseases. seven derivatives of 1 (3 and 5-10) and one derivative of 2 (4) were obtained by chemical modifications of the glycosyl residue, the 2-pyrone ring, the aliphatic ... | 2004 | 15137845 |
| chromium accumulation by living yeast at various environmental conditions. | yeast tolerance to cr (iii) and cr (vi) as well as chromium accumulation potential were shown to depend on treatment time, metal concentration, biomass density and the phase of growth. kinetic studies as exemplified by pichia guilliermondii atcc 201911 revealed a biphasic mode of cr (iii) uptake: a rapid sorption phase was followed by a slow process of accumulation, in which the contribution of the cell-bound cr fraction increased, while the total cellular cr level remained constant. cr (vi) upt ... | 2004 | 15160602 |
| optimized extraction by cetyl trimethyl ammonium bromide reversed micelles of xylose reductase and xylitol dehydrogenase from candida guilliermondii homogenate. | the intracellular enzymes xylose reductase (xr, ec 1.1.1.21) and xylitol dehydrogenase (xd, ec 1.1.1.9) from candida guilliermondii, grown in sugar cane bagasse hydrolysate, were separated by reversed micelles of cetyl trimethyl ammonium bromide (ctab) cationic surfactant. an experimental design was employed to optimize the extraction conditions of both enzymes. under these conditions (temperature = 5 degree c, hexanol: isooctane proportion = 5% (v/v), 22 %, surfactant concentration = 0.15m, ph ... | 2004 | 15177159 |
| liquid-liquid extraction of xylitol dehydrogenase from candida guilliermondii homogenate by reversed micelles. | the intracellular enzyme xylitol dehydrogenase (xd, ec 1.1.1.9) from candida guilliermondii, grown in sugarcane bagasse hydrolysate, was separated by reversed micelles of bdbac [n-benzyl-n-dodecyl-n-bis (2-hydroxyethyl) ammonium chloride] cationic surfactant. an experimental design was employed to evaluate the influence of the following factors on the enzyme separation: temperature, co-solvent concentration and surfactant concentration. the results showed that just the temperature did not show s ... | 2004 | 15177160 |
| a preliminary information about continuous fermentation using cell recycling for improving microbial xylitol production rates(scientific note). | xylitol is a sugar-alcohol with important technological properties, such as anticariogenicity, low caloric value, and negative dissolution heat. it can be used successfully in food formulations and pharmaceutical industries. its production is therefore in great demand. biotechnological xylitol production has several economic advantages in comparison with the conventional process based on the chemical reduction of xylose. the efficiency and the productivity of this fermentation chiefly depends on ... | 1999 | 15304724 |
| xylitol production from wood hydrolyzates by entrapped debaryomyces hansenii and candida guilliermondii cells. | debaryomyces hansenii cells were entrapped in ca-alginate beads and used for producing xylitol from wood hydrolyzates. batch experiments showed that bioconversion was severely hindered when ca-alginate beads were hardened with al3+ solutions. as an alternative to al3+ hardening, the improvements in both mechanical stability of bioparticles and fermenting ability of the immobilized system derived from using increased concentrations of sodium alginate were assessed. the best results were obtained ... | 1999 | 15304779 |
| three new beetle-associated yeast species in the pichia guilliermondii clade. | new yeasts in the pichia guilliermondii clade were isolated from the digestive tract of basidiocarp-feeding members of seven families of coleoptera. a molecular phylogeny and unique traits placed eight isolates in candida fermentati and three undescribed taxa in the genus candida. the new species and type strains are c. smithsonii (type strain nrrl y-27642t), c. athensensis (type strain nrrl y-27644t), and c. elateridarum (type strain nrrl y-27647t). based on comparison of small-and large-subuni ... | 2004 | 15381126 |
| in vitro antifungal activities of anidulafungin and micafungin, licensed agents and the investigational triazole posaconazole as determined by nccls methods for 12,052 fungal isolates: review of the literature. | the echinocandins anidulafungin and micafungin and the triazole posaconazole are currently undergoing phase iii clinical trials. caspofungin and voriconazole have recently been licensed for the treatment of aspergillosis (both agents), other less common mould (voriconazole) and candidal (caspofungin) infections. this review summarizes the published in vitro data obtained by nccls or nccls modified methods on the in vitro fungistatic and fungicidal activities of these five agents for yeasts and m ... | 2003 | 15456349 |
| the distribution frequency of candida species in the genitourinary tract among symptomatic individuals in nigerian cities. | a clinical survey was carried out in seven cities in the southern part of nigeria to determine the relative distribution of genitourinary candida species in symptomatic patients reporting for diagnosis and treatment. seven candida species were identified using the chromagar candida method and the api 20c system. candida species were represented by candida glabrata (33.7%), candida albicans (20.1%), candida tropicalis (18%), candida guilliermondii (17.8%) candida pseudotropicalis (5%), candida pa ... | 2003 | 15456373 |
| phenotypic and genotypic identification of candida spp. isolated from hospitalized patients. | as candidosis incidence continue to rise, quick laboratory identification of candida species is becoming increasingly important for a growing population of patients at-risk. rapd techniques were used on samples of candida obtained from patients hospitalized at santa casa de misericordia in belo horizonte (scmbh) brazil, from march 1998 to december 2000 and then compared with the results of phenotypic identification techniques. two hundred and forty two yeasts were isolated and phenotypically ide ... | 2004 | 15458359 |
| [evaluation of a new chromogenic medium (candida id) for the isolation and presumptive identification of candida albicans and other medically important yeasts]. | candidiasis is a frequent human infection caused mainly by candida albicans. however, other species are emerging as important pathogens, as candida glabrata, candida parapsilosis, candida tropicalis, candida krusei or candida guilliermondii. rapid identification of clinical isolates could facilitate diagnosis and treatment. candida id (biomerieux, spain) is a new medium for the isolation and presumptive identification of yeasts: c. albicans grows as blue colonies, and c. tropicalis, c. guillierm ... | 2001 | 15482010 |
| inter and intra-specific genetic variability of oral candida species. | in this report, strains of five different candida species (candida albicans, candida guilliermondii, candida tropicalis, candida krusei, and candida parapsilosis) isolated from healthy human oral cavities as well as their respective type-strains were used in order to establish the genetic diversity existing among the different species and within a certain species, by the analysis of their electrophoretic alloenzyme patterns. these profiles were analyzed for their band positions in the gels, whic ... | 2001 | 15487908 |
| pyridoxal 5'-phosphate inactivates dna topoisomerase ib by modifying the lysine general acid. | the present results demonstrate that pyridoxal, pyridoxal 5'-phosphate (plp) and pyridoxal 5'-diphospho-5'-adenosine (plp-amp) inhibit candida guilliermondii and human dna topoisomerases i in forming an aldimine with the epsilon-amino group of an active site lysine. plp acts as a competitive inhibitor of c.guilliermondii topoisomerase i (k(i) = 40 microm) that blocks the cleavable complex formation. chemical reduction of plp-treated enzyme reveals incorporation of 1 mol of plp per mol of protein ... | 2004 | 15494452 |
| effect of contraceptives on the prevalence of vaginal colonization with candida species in edo state, nigeria. | high vaginal swabs (hvs) obtained from 500 volunteers in edo state, nigeria which comprised 394 contraceptive users and 106 non-contraceptive users were screened for the prevalence of candida species using standard procedures. results revealed the isolation of candida species in 246 of volunteers. these included candida albicans 174 (38.4%), candida pseudotropicalis 20 (4%), candida stellatoidea 15 (3%), candida krusei nine (1.8%), candida guilliermondii 12 (2.4%), candida tropicalis 11 (2.2%) a ... | 2001 | 15496123 |
| in vitro pharmacodynamic characteristics of amphotericin b, caspofungin, fluconazole, and voriconazole against bloodstream isolates of infrequent candida species from patients with hematologic malignancies. | time-kill and postantifungal effect (pafe) of amphotericin b, caspofungin, fluconazole, and voriconazole were determined against clinical isolates of candida guilliermondii, candida kefyr, and candida lusitaniae. azoles displayed fungistatic activity and no measurable pafe, regardless of the concentration tested. amphotericin b and caspofungin demonstrated concentration-dependent fungicidal activity, although amphotericin b only produced a significant dose-dependent pafe against all isolates tes ... | 2004 | 15504881 |
| survey of yeasts for antagonistic activity against salmonella poona in cantaloupe juice and wounds in rinds coinfected with phytopathogenic molds. | application of yeasts as biocontrol agents to prevent mold decay of fruits and vegetables has been described. we examined 10 yeasts for potential antagonistic activity against survival and growth of salmonella poona in cantaloupe juice and decay by cladosporium cladosporioides and geotrichum candidum in wounds on cantaloupe rind. cantaloupe juice was inoculated using five schemes: salmonella poona only (1.10 log cfu/ml), high (3.93 to 5.21 log cfu/ml) or low populations (1.79 to 3.26 log cfu/ml) ... | 2004 | 15508621 |
| in-vitro activity of 5-fluorocytosine against 1,021 spanish clinical isolates of candida and other medically important yeasts. | the aim of this study was to determine the prevalence of primary resistance to 5-fluorocytosine (5fc) among clinical isolates of yeasts in spain where this drug is not currently available for therapy. we have tested the in vitro activity of 5fc against 1,021 recent yeast clinical isolates, including 522 candida albicans, 140 candida parapsilosis, 68 candida glabrata, 41 candida dubliniensis, 50 candida guilliermondii, 34 candida tropicalis, 28 candida krusei, 20 candida famata, 11 cryptococcus n ... | 2004 | 15538829 |
| candida famata (debaryomyces hansenii) dna sequences containing genes involved in riboflavin synthesis. | previously cloned candida famata (debaryomyces hansenii) strain vkm y-9 genomic dna fragments containing genes rib1 (codes for gtp cyclohydrolase ii), rib2 (encodes specific reductase), rib5 (codes for dimethylribityllumazine synthase), rib6 (encodes dihydroxybutanone phosphate synthase) and rib7 (codes for riboflavin synthase) were sequenced. the derived amino acid sequences of c. famata rib genes showed extensive homology to the corresponding sequences of riboflavin synthesis enzymes of other ... | 2004 | 15543522 |
| initial results from a longitudinal international surveillance programme for anidulafungin (2003). | this longitudinal study evaluated the in vitro activity of anidulafungin against 880 clinical yeast isolates and 68 mould isolates from 64 medical centres in north america, latin america and europe. | 2004 | 15564286 |
| phylogeny and evolution of medical species of candida and related taxa: a multigenic analysis. | hemiascomycetes are species of yeasts within the order saccharomycetales. the order encompasses disparate genera with a variety of life styles, including opportunistic human pathogens (e.g., candida albicans), plant pathogens (e.g., eremothecium gossypii), and cosmopolitan yeasts associated with water and decaying vegetation. to analyze the phylogeny of medically important species of yeasts, we selected 38 human pathogenic and related strains in the order saccharomycetales. the dna sequences of ... | 2004 | 15583292 |
| candida guilliermondii isolated from hiv-infected human secretes a 50 kda serine proteinase that cleaves a broad spectrum of proteinaceous substrates. | non-albicans candida species cause 35-65% of all candidemias in the general population, especially in immunosuppressed individuals. here, we describe a case of a 19-year-old hiv-infected man with pneumonia due to a yeast-like organism. this clinical yeast isolate was identified as candida guilliermondii through mycological tests. c. guilliermondii was cultivated in brain heart infusion medium for 48 h at 37 degrees c. after sequential centrifugation and concentration steps, the free-cell culture ... | 2005 | 15607631 |
| [study of in vitro activity of caspofungin on non-candida albicans yeast strains determined by two methods: m27-a2 and eucast]. | the in vitro activity of caspofungin against 147 non-candida albicans yeasts isolated from blood culture was studied using two broth microdilution methods: m27-a2 and eucast. the minimum concentrations that produced a growth inhibition of > or = 50% and of 100% (mic2 and mic0, respectively) and a reduction in the number of viable colonies > or = 99% versus the initial inoculum (mfc) were determined for all strains. caspofungin demonstrated good activity (mic2 and mic0 < or = 2 mg/l for 90% of th ... | 2004 | 15619655 |
| two new species in the pichia guilliermondii clade: pichia caribbica sp. nov., the ascosporic state of candida fermentati, and candida carpophila comb. nov. | pichia caribbica sp. nov. (type strain dbvpg 4519, nrrl y-27274, cbs 9966) is described as the ascosporic state of candida fermentati, and candida guilliermondii var. carpophila (type strain dbvpg 7739, nrrl y-17905, cbs 5256) is elevated to species status as candida carpophila comb. nov. these new taxa, which are indistinguishable on the basis of conventional taxonomic criteria, differ from one another and from pichia guilliermondii by low dna base sequence relatedness, different electrophoreti ... | 2005 | 15691751 |
| mycological examinations on the fungal flora of the chicken comb. | a total of 500 combs of adult chickens from two different locations in germany (hessen and schleswig-holstein) were clinically and mycologically examined. the chickens came from three battery cages (n = 79), one voliere system (n=32), six flocks maintained on deep litter (n = 69) and 12 flocks kept on free outdoor range (n=320). twenty-two of the 500 chicken combs (4.4%) were found to have clinical signs: only non-specific lesions neither typical of mycosis nor of avian pox such as desquamation ... | 2005 | 15743428 |
| yeast diversity in hypersaline habitats. | thus far it has been considered that hypersaline natural brines which are subjected to extreme solar heating, do not contain non-melanized yeast populations. nevertheless we have isolated yeasts in eight different salterns worldwide, as well as from the dead sea, enriquillo lake (dominican republic) and the great salt lake (utah). among the isolates obtained from hypersaline waters, pichia guilliermondii, debaryomyces hansenii, yarrowia lipolytica and candida parapsilosis are known contaminants ... | 2005 | 15766773 |
| two new fungal inteins. | until recently the only intein known to be encoded by the nuclear genome of a eukaryote was the vma intein in the vacuolar atpase precursor of several species of saccharomycete yeast. this intein has been intensively studied and much information has been gained about its structure, mode of action and evolutionary history. we recently reported a second nuclear intein, cne prp8, encoded within the prp8 gene of the basidiomycete cryptococcus neoformans. subsequent studies have found allelic prp8 in ... | 2005 | 15849795 |
| [selection and properties of mutant yeast pichia guilliermondii strains resistant to chromium (vi)]. | yeast pichia guilliermondii strains l3 and l2, exposed to uv mutagenesis, produced over 80 mutants capable of growing on media containing 1.5 mm bichromate (cr(vi)). the mutations making the strains resistant to cr(vi) were dominant or semidominant. the mutants varied in cr(vi) resistance, the degree of chromium accumulation in the cells (from 0.1 to 11.6 mg/g dry cells), and the degree of cr(vi) reduction (from 50% to complete disappearance of bichromate from the culture liquid). chromium accum ... | 2005 | 15859465 |
| [cloning of structural genes involved in riboflavin synthesis of the yeast candida famata]. | the riboflavin overproducing mutants of the flavinogenic yeast candida famata isolated by conventional selection methods are used for the industrial production of vitamin b2. recently, a transformation system was developed for c. famata using the leu2 mutant as a recipient strain and saccharomyces cerevislae leu2 gene as a selective marker. in this paper the cloning of c. famata genes for riboflavin synthesis on the basis of developed transformation system for this yeast species is described. ri ... | 2004 | 15909421 |
| evaluation of inoculum of candida guilliermondii grown in presence of glucose on xylose reductase and xylitol dehydrogenase activities and xylitol production during batch fermentation of sugarcane bagasse hydrolysate. | the effect of glucose on xylose-xylitol metabolism in fermentation medium consisting of sugarcane bagasse hydrolysate was evaluated by employing an inoculum of candida guilliermondii grown in synthetic media containing, as carbon sources, glucose (30 g/l), xylose (30 g/l), or a mixture of glucose (2 g/l) and xylose (30 g/l). the inoculum medium containing glucose promoted a 2.5-fold increase in xylose reductase activity (0.582 iu/mgprot) and a 2-fold increase in xylitol dehydrogenase activity (0 ... | 2005 | 15917619 |
| sugarcane bagasse as raw material and immobilization support for xylitol production. | xylose-to-xylitol bioconversion was performed utilizing candida guilliermondii immobilized in sugarcane bagasse and cultured in erlenmeyer flasks using sugarcane bagasse hydrolysate as the source of xylose. fermentations were carried out according to a factorial design, and the independent variables considered were treatment, average diameter, and amount of bagasse used as support for cell immobilization. by increasing the amount of support, the xylitol yield decreased, whereas the biomass yield ... | 2005 | 15920271 |
| optimization of acid hydrolysis of sugarcane bagasse and investigations on its fermentability for the production of xylitol by candida guilliermondii. | the dilute-acid hydrolysis of sugarcane bagasse was optimized using a statistical experimental design resulting in hydrolysates containing 57.25 g/l of xylose, which were fermented with a high inoculum concentration (10 g/l of the yeast candida guilliermondii im/ufrj 50088). the addition of urea reduced the time of conversion (tc) to 75 h (without nitrogen source addition tc > 127 h), and, consequently, improving the rates of xylitol bioproduction. fermentator experiments, using the optimized co ... | 2005 | 15920277 |
| positive selection of mutants defective in transcriptional repression of riboflavin synthesis by iron in the flavinogenic yeast pichia guilliermondii. | it is known for many years that iron represses synthesis of riboflavin (rf) and most of rf-synthesizing enzymes in several yeast species, known as flavinogenic yeasts. however, the mechanism of such repression is not known. we have found that iron represses transcription of rib1 and rib7 genes coding for the first and the last enzymes of rf biosynthesis in the model flavinogenic organism pichia guilliermondii. to decipher molecular mechanisms of iron-dependent repression, isolation and study of ... | 2005 | 15925311 |
| a surprisingly large rnase p rna in candida glabrata. | we have found an extremely large ribonuclease p (rnase p) rna (rpr1) in the human pathogen candida glabrata and verified that this molecule is expressed and present in the active enzyme complex of this hemiascomycete yeast. a structural alignment of the c. glabrata sequence with 36 other hemiascomycete rnase p rnas (abbreviated as p rnas) allows us to characterize the types of insertions. in addition, 15 p rna sequences were newly characterized by searching in the recently sequenced genomes cand ... | 2005 | 15987816 |
| kinetic behavior of candida guilliermondii yeast during xylitol production from brewer's spent grain hemicellulosic hydrolysate. | brewer's spent grain, the main byproduct of breweries, was hydrolyzed with dilute sulfuric acid to produce a hemicellulosic hydrolysate (containing xylose as the main sugar). the obtained hydrolysate was used as cultivation medium by candidaguilliermondii yeast in the raw form (containing 20 g/l xylose) and after concentration (85 g/l xylose), and the kinetic behavior of the yeast during xylitol production was evaluated in both media. assays in semisynthetic media were also performed to compare ... | 2005 | 16080723 |
| malassezia and candida colonisation on glans penis of circumcised men. | the malassezia yeast are members of the normal human cutaneous flora in adults. they also are reported as part of the microflora of the male genital region in mostly uncircumcised males. it has been reported that malassezia sympodialis and malassezia globosa are the most frequent yeast belonging to the resident microflora of the penis as in other human skin areas. the aim was to evaluate the prevalence of malassezia and candida yeast colonisation on the glans penis of circumcised males. impressi ... | 2005 | 16115108 |
| purification of xylitol from fermented hemicellulosic hydrolyzate using liquid-liquid extraction and precipitation techniques. | xylitol was produced by candida guilliermondii by fermentation of sugarcane bagasse hemicellulosic hydrolysate. undesirable impurities were extracted from the broth using either ethyl acetate, chloroform or dichloromethane. the best results on clarification of the broth without xylitol loss were obtained with ethyl acetate. when ethanol, acetone or tetrahydrofuran were used for precipitation of impurities, only tetrahydrofuran clarified the fermented broth, but a high xylitol loss (approximately ... | 2005 | 16132861 |
| development of a dna microarray for detection and identification of fungal pathogens involved in invasive mycoses. | invasive fungal infections have emerged as a major cause of morbidity and mortality in immunocompromised patients. conventional identification of pathogenic fungi in clinical microbiology laboratories is time-consuming and, therefore, often imperfect for the early initiation of an adequate antifungal therapy. we developed a diagnostic microarray for the rapid and simultaneous identification of the 12 most common pathogenic candida and aspergillus species. oligonucleotide probes were designed by ... | 2005 | 16207946 |
| molecular typing of the yeast species dekkera bruxellensis and pichia guilliermondii recovered from wine related sources. | a total of 63 strains of dekkera bruxellensis and 32 strains of pichia guilliermondii isolated from wine related environments were identified by restriction analysis of the 5.8s-its region of the rdna. these strains were subjected to intraspecific discrimination using mtdna restriction and rapd-pcr analysis. the isolates identified as d. bruxellensis yielded 3 different molecular patterns of mtdna restriction using the endonuclease hinfi. the pattern a was the most frequent (58 strains) among st ... | 2006 | 16229917 |
| vulvovaginal candidiasis in a flemish patient population. | increased resistance to fluconazole has been reported in oral, oesophageal and urinary candida isolates, but this has not been observed commonly in genital tract isolates. the rate of isolation of candida spp. and their susceptibility to amphotericin b, flucytosine and azoles were determined in a number of clinical practices in the city of ghent, belgium. patients with symptomatic vulvovaginal candidiasis (vvc) were treated with fluconazole, and the mycological and clinical outcomes were evaluat ... | 2005 | 16307555 |
| occurrence and diversity of yeasts in the mid-atlantic ridge hydrothermal fields near the azores archipelago. | the yeast community associated with deep-sea hydrothermal systems of the mid-atlantic rift was surveyed for the first time. this study relied on a culture-based approach using two different growth media: a conventional culture medium for yeasts supplemented with sea salts (mypss) and the same medium additionally supplemented with sulfur (mypsss). for the evaluation of species diversity, a molecular approach involving minisatellite-primed polymerase chain reaction (msp-pcr) strain typing and sequ ... | 2005 | 16328655 |
| yeast community survey in the tagus estuary. | the yeast community in the waters of the tagus estuary, portugal, was followed for over a year in order to assess its dynamics. yeast occurrence and incidence were measured and this information was related to relevant environmental data. yeast occurrence did not seem to depend upon tides, but river discharge had a dramatic impact both on the density and diversity of the community. the occurrence of some yeasts was partially correlated with faecal pollution indicators. yeast isolates were charact ... | 2005 | 16329949 |
| isolation of plasmid-harboring serratia plymuthica from facultative gut microflora of the tobacco hornworm, manduca sexta. | aseptic isolation of the facultative gut microflora of the tobacco hornworm, manduca sexta, yielded four microorganisms. two were gram-positive bacillus spp., one was serratia plymuthica, and another was the yeast candida guilliermondii. the three bacterial species were screened for extrachromosomal dna, and s. plymuthica was found to have a 6.4-kilobase plasmid, which was designated pcp-1. | 1988 | 16347696 |
| characterization and reclassification of yeasts used for biological control of postharvest diseases of fruits and vegetables. | in previous studies workers have shown that three yeast strains (strains us-7, 82, and 101) have biological control activity against various postharvest fungal pathogens of fruits and vegetables, including penicillium rots of apples and citrus and botrytis rot of apples. in these reports the researchers have described these strains as debaryomyces hansenii (anamorph, candida famata) or candida sp. strains. in this study we performed additional physiological, dna reassociation, and mannan charact ... | 1990 | 16348361 |
| successive microbial populations in calimyrna figs. | smyrna-type (calimyrna) figs have essentially sterile internal tissue until visited by the pollinating fig wasp, blastophaga psenes, which introduces a specific microflora consisting of candida guilliermondii var. carpophila and serratia plymuthica. this flora persists and develops in numbers throughout the ripening period until maturity of the fruit. these organisms do not cause spoilage. the presence of c. guilliermondii var. carpophila appears to increase the attractiveness of the fruit to dr ... | 1962 | 16349620 |
| human recombinant antimannan immunoglobulin g1 antibody confers resistance to hematogenously disseminated candidiasis in mice. | mannan is a major cell wall component found in candida species. natural antimannan antibody is present in sera from most normal adults, but its role in host resistance to hematogenously disseminated candidiasis is unknown. the purpose of this study was to develop recombinant human antimannan antibody and to study its protective function. a phage fab display combinatorial library containing fab genes from bone marrow lymphocytes was screened with candida albicans yeast cells and chemically purifi ... | 2006 | 16368991 |
| structural changes of cell wall mannans of candida guilliermondii ifo 10279 strain cells cultured at high temperature. | the morphology, structure, and antigenicity of the cells and the cell wall mannans of the candida guilliermondii ifo 10279 strain cultivated at 33 and 34 degrees c for 48 h in yeast extract-added sabouraud liquid medium (yslm) were compared with those cultivated at 27 degrees c and 33 degrees c and then at 27 degrees c (33-27 degrees c). this strain showed little growth at higher than 35 degrees c. the density of the yeast formed cells decreased, with dry weights of about 50% at 33 and 34 degree ... | 2006 | 16462053 |
| application of sscp-pcr fingerprinting to profile the yeast community in raw milk salers cheeses. | bacteria and yeasts are important sensory factors of raw-milk cheeses as they contribute to the sensory richness and diversity of these products. the diversity and succession of yeast populations in three traditional registered designation of origin (r.d.o.) salers cheeses have been determined by using phenotypic diagnoses and single-strand conformation polymorphism (sscp) analysis. isolates were identified by phenotypic tests and the sequencing of the d1-d2 domains of the 26s rrna gene. ninety- ... | 2006 | 16464699 |
| rapid identification of fungal pathogens in bact/alert, bactec, and bbl mgit media using polymerase chain reaction and dna sequencing of the internal transcribed spacer regions. | we report a direct polymerase chain reaction/sequence (d-pcrs)-based method for the rapid identification of clinically significant fungi from 5 different types of commercial broth enrichment media inoculated with clinical specimens. media including bact/alert fa (biomérieux, marcy l'etoile, france) (n = 87), bactec plus aerobic/f (becton dickinson, microbiology systems, sparks, md) (n = 16), bactec peds plus/f (becton dickinson) (n = 15), bactec lytic/10 anaerobic/f (becton dickinson) (n = 11) b ... | 2006 | 16466900 |
| biocontrol of mold growth in high-moisture wheat stored under airtight conditions by pichia anomala, pichia guilliermondii, and saccharomyces cerevisiae. | volume 61, no. 3, p. 1029, table 1, boxhead over columns 2 to 4: "(cfu/plate)" should read "(log cfu/plate)." fig. 1 [this corrects the article on p. 1027 in vol. 61.]. | 1995 | 16535015 |
| fermentation performance of candida guilliermondii for xylitol production on single and mixed substrate media. | semidefined media fermentation simulating the sugar composition of hemicellulosic hydrolysates (around 85 g l(-1) xylose, 17 g l(-1) glucose, and 9 g l(-1) arabinose) was investigated to evaluate the glucose and arabinose influence on xylose-to-xylitol bioconversion by candida guilliermondii. the results revealed that glucose reduced the xylose consumption rate by 30%. arabinose did not affect the xylose consumption but its utilization by the yeast was fully repressed by both glucose and xylose ... | 2006 | 16541249 |
| occurrence of yeasts in cloacae of migratory birds. | several species of yeast have been reported as pathogens in humans based on increases in immunodeficiency syndromes and as a result of immunosuppressant chemotherapy in cancer treatment. domestic and wild birds are known to act as carriers of human pathogenic fungi. to gain additional information on the yeasts present in the cloacae of some species of migratory birds, 421 wild birds (24.39% out of 1726 birds caught in romania, hungary and bulgaria) were sampled with the permission of the local j ... | 2006 | 16552486 |
| effect of phosphate buffer concentration on the batch xylitol production by candida guilliermondii. | to evaluate the effect of phosphate buffer concentration on growth and xylitol production by candida guilliermondii fti 20037. | 2006 | 16599982 |
| in vitro anticandidal activity of xanthorrhizol isolated from curcuma xanthorrhiza roxb. | xanthorrhizol, isolated from the methanol extract of curcuma xanthorrhiza roxb., was investigated for its anticandidal activity using six candida species. | 2006 | 16617064 |
| multiple gene genealogical analyses suggest divergence and recent clonal dispersal in the opportunistic human pathogen candida guilliermondii. | candida guilliermondii is a haploid opportunistic pathogen accounting for about 2 % of human blood yeast infections. recent analyses using multilocus enzyme electrophoresis and karyotyping suggest that strains from human sources traditionally designated c. guilliermondii in fact include at least two species, c. guilliermondii and candida fermentati. however, the patterns of molecular variation within and between these two species remain largely unknown. in this study, dna fragments were sequence ... | 2006 | 16622071 |
| enhanced xylitol production by precultivation of candida guilliermondii cells in sugarcane bagasse hemicellulosic hydrolysate. | the present work evaluated the key enzymes involved in xylitol production (xylose reductase [xr] and xylitol dehydrogenase [xdh]) and their correlation with xylose, arabinose, and acetic acid assimilation during cultivation of candida guilliermondii fti 20037 cells in sugarcane bagasse hemicellulosic hydrolysate. for this purpose, inocula previously grown either in sugarcane bagasse hemicellulosic hydrolysate (sbhh) or in semidefined medium (xylose as a substrate) were used. the highest xylose/a ... | 2006 | 16775788 |
| candida guilliermondii fungemia in patients with hematologic malignancies. | the microbiological, clinical, and epidemiological features of most non-candida albicans candida species are well known, but much less is known about species such as candida guilliermondii, an uncommon pathogen causing a variety of deep-seated infections in immunocompromised hosts. to characterize c. guilliermondii fungemia in patients with hematological malignancies and its susceptibility to antifungal drugs, all cases of c. guilliermondii fungemia diagnosed in our department between 1983 and 2 ... | 2006 | 16825364 |
| candida guilliermondii as the aetiology of candidosis. | candida guilliermondii is one of the components of human microbiota. this yeast has been infrequently associated with human infections, which may be related to its low pathogenicity. the aim of this study was to provide clinical and epidemiological data for patients infected with c. guilliermondii at santa casa complexo hospitalar, brazil. from october 1997 to october 2003, c. guilliermondii was isolated from clinical samples from 11 patients. three patients were excluded because the isolation o ... | 2006 | 16847499 |
| [in vitro resistance to fluconazole and itraconazole in clinical isolates of candida spp and cryptococcus neoformans]. | an in vitro susceptibility testing of 181 strains of six species of candida and 21 strains of cryptococcus neoformans was carried out in order to investigate the resistance to new antifungal drugs. we have studied clinical isolates from 200 different patients of hospital del mar (barcelona) and hospital la inmaculada (almería). an agar diffusion method (neosensitabs, rosco, taastrup, denmark), was employed with fluconazole, itraconazole, and reference drugs amphotericin b, flucytosine, tioconazo ... | 1997 | 16854170 |
| effects of caspofungin against candida guilliermondii and candida parapsilosis. | the in vitro activity of caspofungin (cas) was investigated against 28 yeast isolates belonging to candida albicans (n = 5), candida guilliermondii (n = 10), and candida parapsilosis (n = 13). cas mics obtained by broth dilution and etest methods clearly showed a rank order of susceptibility to the echinocandin compound with c. albicans > c. parapsilosis > c. guilliermondii. similarly, time-kill assays performed on selected isolates showed that cas was fungistatic against c. albicans and c. para ... | 2006 | 16870764 |
| killing kinetics of caspofungin, micafungin, and amphotericin b against candida guilliermondii. | amphotericin b (amb), micafungin, and caspofungin mics, minimal fungicidal concentrations, and time-killing curves were determined in the presence and absence of 10% inactivated serum. amb was the only agent with consistent killing activity (time required to achieve 99.9% of growth reduction was 2.1 to 3.2 h). the presence of serum enhanced caspofungin activity but lowered those of micafungin and amb. | 2006 | 16870779 |
| candida albicans and candida glabrata clinical isolates exhibiting reduced echinocandin susceptibility. | a recognized hotspot for mutations conferring reduced echinocandin susceptibility (res) is residue s645 of candida albicans gsc1(fks1). we report that the mutation f641y is associated with res in a c. albicans isolate. the analogous fks2 residue is mutated f to v in a candida glabrata res isolate; the introduction of this mutation into susceptible c. glabrata confirmed its role in res. y641-equivalent fks residues were identified in intrinsically res fusarium species and candida guilliermondii. | 2006 | 16870797 |
| prevalence of potentially pathogenic fungi in the bathing sites of the sulejów reservoir. | progressive degradation of water ecosystems may contribute to the propagation of fungi pathogenic to humans and animals. the aim of this study was a quantitative evaluation and identification of species of potentially pathogenic fungi in the littoral zone of the sulejów reservoir used as recreation site. we studied samples of surface water and sediments collected from 6 sampling sites of the reservoir in the years 2000 and 2001. in 2000, fungi were isolated from 82.7% of samples, while in 2001 f ... | 2003 | 16889024 |
| rsm analysis of the effects of the oxygen transfer coefficient and inoculum size on the xylitol production by candida guilliermondii. | biotechnology production of xylitol is an excellent alternative to the industrial chemical process for the production of this polyalcohol. in this work the behavior of candida guilliermondii yeast was studied when crucial process variables were modified. the k(l)a (between 18 and 40/h) and the initial cell mass (between 4 and 10 g) were considered as control variables. a response surface methodology was applied to the experimental design to study the resulting effect when the control variables w ... | 2006 | 16915645 |
| survey of amphotericin b susceptibility of candida clinical isolates determined by etest. | the minimal inhibitory concentrations (mics) of amphotericin b (amb) determined by the national committee for clinical laboratory standards (nccls; nccls document m27-a) broth dilution method are in a relatively narrow ranges and this may lead to underestimation of the amb-resistant rate in clinical isolates. we evaluated in vitro susceptibility of clinical isolates of candida spp. to amb using etest and determined the distribution of amb mics in different species. | 2006 | 16926981 |
| application of pcr-dgge to analyse the yeast population dynamics in slurry reactors during degradation of polycyclic aromatic hydrocarbons in weathered oil. | slurry-phase reactors have been used to investigate the biodegradation feasibility of polycyclic aromatic hydrocarbons (pahs) in weathered crude oil, by mixed culture containing five pahs-degrading yeast strains. yeasts were isolated from the oily soil by enrichment culture, using phenanthrene as a sole carbon source, and identified based on the 26s ribosomal dna (rdna) sequence. yeast strains belonged to the genera candida, pichia, rhodotorula and sporidiobolus. the experiment was carried out f ... | 2006 | 17001616 |
| determination of mics of aminocandin for candida spp. and filamentous fungi. | candida and aspergillus spp., as well as other filamentous molds, have increasingly been reported as the causes of severe invasive fungal infections. we evaluated the new echinocandin aminocandin (amn) for its antifungal activities against a range of fungal pathogens by determination of the mics for the organisms. the mics of the comparator drugs amphotericin b, caspofungin, micafungin, and voriconazole were also determined. the mics of amn for 25 strains each of non-candida albicans candida spp ... | 2006 | 17021057 |
| global surveillance of in vitro activity of micafungin against candida: a comparison with caspofungin by clsi-recommended methods. | micafungin is an echinocandin antifungal agent that has recently been approved for the prevention of invasive fungal infection and the treatment of esophageal candidiasis. prospective sentinel surveillance for the emergence of in vitro resistance to micafungin among invasive candida sp. isolates is indicated. we determined the in vitro activity of micafungin against 2,656 invasive (bloodstream or sterile site) unique patient isolates of candida spp. collected from 60 medical centers worldwide in ... | 2006 | 17021079 |
| candida guilliermondii, an opportunistic fungal pathogen with decreased susceptibility to fluconazole: geographic and temporal trends from the artemis disk antifungal surveillance program. | although a rare cause of invasive candidiasis, candida guilliermondii has been reported to exhibit decreased susceptibility to antifungal agents. aside from case reports and small surveys, there is little information regarding the epidemiology and antifungal susceptibility profile of c. guilliermondii. we report geographic and temporal trends in the isolation and antifungal susceptibilities of 1,029 c. guilliermondii clinical isolates collected from 127 medical centers as part of the artemis dis ... | 2006 | 17021081 |
| screening and characterization of yeasts for xylitol production. | to discover novel naturally occurring xylitol producing yeast species with potential for industrial applications. | 2006 | 17040233 |
| inulinase production by a marine yeast pichia guilliermondii and inulin hydrolysis by the crude inulinase. | marine yeast strain 1, isolated from the surface of a marine alga, was found to secrete a large amount of inulinase into the medium. this marine yeast was identified as a strain of pichia guilliermondii according to the results of routine yeast identification and molecular methods. the crude inulinase produced by this marine yeast worked optimally at ph 6.0 and 60 degrees c. the optimal medium for inulinase production was seawater containing 4.0% (w/v) inulin and 0.5% (w/v) yeast extract, while ... | 2007 | 17111131 |
| molecular modelling studies on the interactions of human dna topoisomerase ib with pyridoxal-compounds. | candida guilliermondii and human dna topoisomerases i are inhibited by pl (pyridoxal), plp (pyridoxal 5'-phosphate) and plp-amp (pyridoxal 5'-diphospho-5'-adenosine) (pl<plp<plp-amp). we have recently shown that plp acted as a competitive inhibitor of c. guilliermondii topoisomerase i, impeding the formation of the cleavable complex from a selective binding to an active site lysine. the targeted lysine in c. guilliermondii topoisomerase i occupies a position equivalent to that of lysine 532 (k(5 ... | 2007 | 17116355 |
| a fungal phylogeny based on 42 complete genomes derived from supertree and combined gene analysis. | to date, most fungal phylogenies have been derived from single gene comparisons, or from concatenated alignments of a small number of genes. the increase in fungal genome sequencing presents an opportunity to reconstruct evolutionary events using entire genomes. as a tool for future comparative, phylogenomic and phylogenetic studies, we used both supertrees and concatenated alignments to infer relationships between 42 species of fungi for which complete genome sequences are available. | 2006 | 17121679 |
| [susceptibility of fungi isolated from clinical materials to voriconazole]. | the objective of the study was evaluation of the susceptibility of 139 fungal strains isolated from clinical materials to voriconazole, a new antifungal agent of the triazole group. a dilution method was used. the drug was incorporated into the culture medium at concentrations 0.1-100 mg/l. it was found out that the antifungal effectiveness of the drug varied both between various fungal genera and species, and between strains within the same species. total inhibition of the growth of 20% of the ... | 2006 | 17133909 |
| repeated-batch xylitol bioproduction using yeast cells entrapped in polyvinyl alcohol-hydrogel. | xylose-to-xylitol conversion was investigated in a bench-scale bioreactor using candida guilliermondii cells entrapped within polyvinyl alcohol-hydrogel beads in a system operated in repeated-batch mode with cell recycling. yeast-viable cells were immobilized in the support using the freezing-thawing method. bioconversion assays were performed in a stirred tank reactor operated at 400-rpm agitation speed, 30 degrees c temperature, and 1.04-vvm air flow rate. the system was explored during six su ... | 2007 | 17211545 |
| evidence for a pseudo-outbreak of candida guilliermondii fungemia in a university hospital in brazil. | fungal infections due to candida species represent an important cause of nosocomial bloodstream infections. we report a large pseudo-outbreak of candida guilliermondii fungemia that occurred in a university hospital in brazil. c. guilliermondii was identified in 64 (43%) of the 149 blood samples drawn between june 2003 and july 2004. the samples were from patients in different wards of the hospital but concentrated in pediatric units. none of the patients had clinical signs of fungemia, and obse ... | 2007 | 17229862 |
| antifungal activity of brazilian medicinal plants involved in popular treatment of mycoses. | a survey of medicinal plants used to treat common mycoses was done in the curituba district, sergipe state, brazil. one hundred inhabitants were interviewed by health agents and traditional healers. four different plants were the most cited (more than 50% of the citations): ziziphus joazeiro, caesalpinia pyramidalis, bumelia sartorum and hymenea courbaril. the aqueous extracts obtained following traditional methods and using different parts of these plants, were submitted to drop agar diffusion ... | 2007 | 17234376 |
| physiological characterization of spoilage strains of zygosaccharomyces bailii and zygosaccharomyces rouxii isolated from high sugar environments. | two isolates of spoilage yeasts zygosaccharomyces bailii and zygosaccharomyces rouxii were obtained from a high sugar environment, in a factory producing candied fruit and nougat. other strains, z. bailii from other environments and other isolates from high sugar/salt environments were obtained for comparison (zygosaccharomyces lentus, candida magnoliae, candida halophila and pichia guilliermondii). a full physiological assessment of these isolates was carried out, of preservative and biocide re ... | 2007 | 17239464 |
| antifungal susceptibility of 1000 candida bloodstream isolates to 5 antifungal drugs: results of a multicenter study conducted in são paulo, brazil, 1995-2003. | we evaluated all candida sp. bloodstream isolates obtained from patients admitted to 4 tertiary care hospitals between 1995 and 2003 in the city of são paulo, brazil. susceptibility to amphotericin b, 5-fluorocytosine, fluconazole (fcz), itraconazole (itz), and voriconazole (vcz) was determined using the clinical laboratory standards institute broth microdilution method. we tested a total of 1000 strains, including 400 strains of candida albicans (40%), 243 of candida tropicalis (24.3%), 238 of ... | 2007 | 17240110 |
| pcr-based identification and strain typing of pichia anomala using the ribosomal intergenic spacer region igs1. | frequent outbreaks of pichia anomala fungaemia in paediatric patients have warranted the development of a rapid identification system for this organism. this study describes a specific pcr-based method targeting the rrna gene intergenic spacer region 1 (igs1) for rapid identification of pichia anomala isolates and characterization at the strain level. these methods of species identification and strain typing were used on 106 isolates of pichia anomala (77 from a previously described outbreak and ... | 2007 | 17244798 |
| l-arabinose metabolism in candida arabinofermentans pycc 5603t and pichia guilliermondii pycc 3012: influence of sugar and oxygen on product formation. | l-arabinose utilization by the yeasts candida arabinofermentans pycc 5603(t) and pichia guilliermondii pycc 3012 was investigated in aerobic batch cultures and compared, under similar conditions, to d-glucose and d-xylose metabolism. at high aeration levels, only biomass was formed from all the three sugars. when oxygen became limited, ethanol was produced from d-glucose, demonstrating a fermentative pathway in these yeasts. however, pentoses were essentially respired and, under oxygen limitatio ... | 2007 | 17262211 |