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genome- wide characterization of nuclear factor y (nf-y) gene family of sorghum [sorghum bicolor (l.) moench]: a bioinformatics approach.nuclear factor y (nf-y) is a heterotrimeric transcription factor (tf) complex with preferential binding to ccaat elements of promoters, regulating gene expression in most of the higher eukaryotes. the availability of plant genome sequences have revealed multiple number of genes coding for the three subunits, namely nf-ya, nf-yb and nf-yc in contrast to single nf-y gene for each subunit reported in yeast and animals. a total of 33 nf-ytf comprising of 8 nf-ya, 11 nf-yb and 14 nf-yc subunits were ...201627186017
using genotyping by sequencing to map two novel anthracnose resistance loci in sorghum bicolor.colletotrichum sublineola is an aggressive fungal pathogen that causes anthracnose in sorghum [sorghum bicolor (l.) moench]. the obvious symptoms of anthracnose are leaf blight and stem rot. sorghum, the fifth most widely grown cereal crop in the world, can be highly susceptible to the disease, most notably in hot and humid environments. in the southeastern united states the acreage of sorghum has been increasing steadily in recent years, spurred by growing interest in producing biofuels, bio-ba ...201627194807
a foxtail mosaic virus vector for virus-induced gene silencing in maize.plant viruses have been widely used as vectors for foreign gene expression and virus-induced gene silencing (vigs). a limited number of viruses have been developed into viral vectors for the purposes of gene expression or vigs in monocotyledonous plants, and among these, the tripartite viruses brome mosaic virus and cucumber mosaic virus have been shown to induce vigs in maize (zea mays). we describe here a new dna-based vigs system derived from foxtail mosaic virus (fomv), a monopartite virus t ...201627208311
review of anthraquinone applications for pest management and agricultural crop protection.we have reviewed published anthraquinone applications for international pest management and agricultural crop protection from 1943 to 2016. anthraquinone (aq) is commonly found in dyes, pigments and many plants and organisms. avian repellent research with aq began in the 1940s. in the context of pest management, aq is currently used as a chemical repellent, perch deterrent, insecticide and feeding deterrent in many wild birds, and in some mammals, insects and fishes. criteria for evaluation of e ...201627252044
genome-wide characterization of major intrinsic proteins in four grass plants and their non-aqua transport selectivity profiles with comparative perspective.major intrinsic proteins (mips), commonly known as aquaporins, transport not only water in plants but also other substrates of physiological significance and heavy metals. in most of the higher plants, mips are divided into five subfamilies (pips, tips, nips, sips and xips). herein, we identified 68, 42, 38 and 28 full-length mips, respectively in the genomes of four monocot grass plants, specifically panicum virgatum, setaria italica, sorghum bicolor and brachypodium distachyon. phylogenetic an ...201627327960
genome-wide association mapping of quantitative traits in a breeding population of sugarcane.molecular markers associated with relevant agronomic traits could significantly reduce the time and cost involved in developing new sugarcane varieties. previous sugarcane genome-wide association analyses (gwas) have found few molecular markers associated with relevant traits at plant-cane stage. the aim of this study was to establish an appropriate gwas to find molecular markers associated with yield related traits consistent across harvesting seasons in a breeding population. sugarcane clones ...201627342657
rapid and efficient agrobacterium-mediated transformation of sorghum (sorghum bicolor) employing standard binary vectors and bar gene as a selectable marker.a rapid and efficient agrobacterium -mediated transformation system in sorghum has been developed employing standard binary vectors and bar gene as a selectable marker. sorghum (sorghum bicolor) is an important food and biofuel crop worldwide, for which improvements in genetic transformation are needed to study its biology and facilitate agronomic and commercial improvement. here, we report optimization of regeneration and transformation of public sorghum genotype p898012 using standard binary v ...201627350252
amino-substituted para-benzoquinones as potential herbicides.although quinones present a large array of biological activities, a few studies on the herbicidal potential of 2,5-bis(alkyl/arylamino)-1,4-benzoquinones have been reported to date. in this work, starting from benzoquinone, 13 2,5-bis(alkyl/arylamino)-1,4-benzoquinones were prepared in 46 - 93% yield. the products were fully characterized by spectroscopic analyses and their phytotoxicity against cucumis sativus and sorghum bicolor seedlings was investigated. at 100 ppm, compounds caused 10 - 88% ...201627389616
decomposition, nitrogen and carbon mineralization from food and cover crop residues in the central plateau of haiti.cover crops are a major focus of conservation agriculture efforts because they can provide soil cover and increase nutrient availability after their mineralization in cropping systems. to evaluate the effect of residue type and placement on rate of decomposition and carbon (c) and nitrogen (n) mineralization, residues from two food crops, maize (zea mays l.) and common bean (phaseolus vulgaris l.), and two promising cover crops, sunn hemp (crotalaria juncea l.) and sorghum sudangrass (sorghum bi ...201627429883
the effects of simultaneous application of plant growth regulators and bioaugmentation on improvement of phytoremediation of pyrene contaminated soils.polycyclic aromatic hydrocarbons (pahs) refer to a wide group of soil contaminants whose presence in the environment is a cause of concern. the present study aimed to evaluate the effects of indole acetic acid (iaa) and pseudomonas aeruginosa on the phytoremediation activities of sorghum bicolor and increase in pyrene removal efficiency in the soil. the initial concentration of pyrene was 150 and 300 mg kg(-1) in this experiment. the treatments included unplanted soil (t0), planted soil with sor ...201627434251
single nucleotide polymorphism discovery via genotyping by sequencing to assess population genetic structure and recurrent polyploidization in andropogon gerardii.autopolyploidy, genome duplication within a single lineage, can result in multiple cytotypes within a species. geographic distributions of cytotypes may reflect the evolutionary history of autopolyploid formation and subsequent population dynamics including stochastic (drift) and deterministic (differential selection among cytotypes) processes. here, we used a population genomic approach to investigate whether autopolyploidy occurred once or multiple times in andropogon gerardii, a widespread, n ...201627466055
complete chloroplast genome sequences of hordeum vulgare, sorghum bicolor and agrostis stolonifera, and comparative analyses with other grass genomes. 200727520091
differential responses of soybean and sorghum growth, nitrogen uptake, and microbial metabolism in the rhizosphere to cattle manure application: a rhizobox study.in this study, we determined the capacity of soybean (glycine max l. merr. cv. hoyoharuka) and sorghum (sorghum bicolor l. moench. cv. hybrid sorgo) to utilize different forms of nitrogen (n) in a rhizobox system. seedlings were grown for 35 days without n or with 130 mg n kg(-1) soil as ammonium sulfate or farmyard cattle manure. the soil fractions at different distances from the root were sliced millimeter by millimeter in the rhizobox system. we assessed the distribution of different forms of ...201627726362
nj cluster analysis of the snrk2, pyr/pyl/rcar, and abf genes in tibetan hulless barley.the abscisic acid (aba) signaling pathway is known as one of the most important signaling pathways in plants and is mediated by multiple regulators. the genes snrk2, pyr/pyl/rcar, and abf are relevant to both aba-dependent and -independent signaling pathways. to elucidate the profile of these genes from tibetan hulless barley (hordeum vulgare l. var. nudum hook. f.), we collected available sequences from rna-seq data, together with ncbi data from five other model plant species (arabidopsis thali ...201627819745
insect and plant-derived mirnas in greenbug (schizaphis graminum) and yellow sugarcane aphid (sipha flava) revealed by deep sequencing.schizaphis graminum (green bug; gb) and sipha flava (yellow sugarcane aphid; ysa) are two cereal aphid species with broad host ranges capable of establishing on sorghum (sorghum bicolor) and several switchgrass (panicum virgatum) cultivars. switchgrass and sorghum are staple renewable bioenergy crops that are vulnerable to damage by aphids, therefore, identifying novel targets to control aphids has the potential to drastically improve yields and reduce losses in these bioenergy crops. despite th ...201727838454
effect of feeding warm-season annuals with orchardgrass on ruminal fermentation and methane output in continuous culture.a 4-unit, dual-flow continuous culture fermentor system was used to assess nutrient digestibility, volatile fatty acids (vfa) production, bacterial protein synthesis, and methane (ch4) output of warm-season annual grasses. treatments were randomly assigned to fermentors in a 4 × 4 latin square design using 7 d for adaptation to treatment and 3 d for sample collection. treatments were (1) 100% orchardgrass (dactylis glomerata l.; ord); (2) 50% orchardgrass + 50% japanese millet [echinochloa escul ...201727939544
sucrose transporter localization and function in phloem unloading in developing stems.how sucrose transporters (suts) regulate phloem unloading in monocot stems is poorly understood and particularly so for species storing high suc concentrations. to this end, sorghum bicolor suts sbsut1 and sbsut5 were characterized by determining their transport properties heterologously expressed in yeast or xenopus laevis oocytes, and their in planta cellular and subcellular localization. the plasma membrane-localized sbsut1 and sbsut5 exhibited a strong selectivity for suc and high suc affini ...201727986867
effects of untreated and treated wastewater at the morphological, physiological and biochemical levels on seed germination and development of sorghum (sorghum bicolor (l.) moench), alfalfa (medicago sativa l.) and fescue (festuca arundinacea schreb.).wastewater reuse in agriculture may help mitigate water scarcity. this may be reached if high quality treatments removing harmful pollutants are applied. the aim of the present study was to compare the effect of untreated (utw) and treated wastewater (tw) on germination and seedlings development of alfalfa (medicago sativa l.), fescue (festuca arundinacea schreb.) and sorghum (sorghum bicolor (l.) moench). utw presented high turbidity (130 ntu), chemical and biological oxygen demand (cod, 719mgl ...201728013160
catchup: a web database for spatiotemporally regulated genes.for proper control of biological activity, some key genes are highly expressed in a particular spatiotemporal domain. mining of such spatiotemporally expressed genes using large-scale gene expression data derived from a broad range of experimental sources facilitates our understanding of genome-scale functional gene networks. however, comprehensive information on spatiotemporally expressed genes is lacking in plants. to collect such information, we devised a new index, δdmax, which is the maximu ...201728013273
evolution and structural diversification of nictaba-like lectin genes in food crops with a focus on soybean (glycine max).the nictaba family groups all proteins that show homology to nictaba, the tobacco lectin. so far, nictaba and an arabidopsis thaliana homologue have been shown to be implicated in the plant stress response. the availability of more than 50 sequenced plant genomes provided the opportunity for a genome-wide identification of nictaba-like genes in 15 species, representing members of the fabaceae, poaceae, solanaceae, musaceae, arecaceae, malvaceae and rubiaceae. additionally, phylogenetic relations ...201728087663
the primary root of sorghum bicolor (l. moench) as a model system to study brassinosteroid signaling in crops.roots anchor plants to the soil and are essential for a successful plant growth and adaptation to the environment. research on the primary root in the plant model system arabidopsis thaliana has yielded important advances in the molecular and cellular understanding of root growth and development. several studies have uncovered how the hormones brassinosteroids (brs) control cell cycle and differentiation programs through different cell-specific signaling pathways that are key for root growth and ...201728124255
casul: a new lectin isolated from calliandra surinamensis leaf pinnulae with cytotoxicity to cancer cells, antimicrobial activity and antibiofilm effect.this work describes the isolation of a lectin (casul) from the leaf pinnulae of calliandra surinamensis and the evaluation of its cytotoxic, antimicrobial and antibiofilm properties. proteins from pinnulae extract were precipitated with ammonium sulphate (60% saturation) and submitted to sephadex g-75 chromatography, which yielded isolated casul (purification factor: 113). native casul is an acidic protein (pi 5.82) with a relative molecular mass of 48kda. this lectin is also an oligomeric prote ...201728174088
biochemical characterization of metabolism-based atrazine resistance in amaranthus tuberculatus and identification of an expressed gst associated with resistance.rapid detoxification of atrazine in naturally tolerant crops such as maize (zea mays) and grain sorghum (sorghum bicolor) results from glutathione s-transferase (gst) activity. in previous research, two atrazine-resistant waterhemp (amaranthus tuberculatus) populations from illinois, u.s.a. (designated acr and mcr), displayed rapid formation of atrazine-glutathione (gsh) conjugates, implicating elevated rates of metabolism as the resistance mechanism. our main objective was to utilize protein pu ...201728218978
transcript profiling reveals the presence of abiotic stress and developmental stage specific ascorbate oxidase genes in plants.abiotic stress and climate change is the major concern for plant growth and crop yield. abiotic stresses lead to enhanced accumulation of reactive oxygen species (ros) consequently resulting in cellular damage and major losses in crop yield. one of the major scavengers of ros is ascorbate (aa) which acts as first line of defense against external oxidants. an enzyme named ascorbate oxidase (aao) is known to oxidize aa and deleteriously affect the plant system in response to stress. genome-wide an ...201728261251
the wheat lr34 multi-pathogen resistance gene confers resistance to anthracnose and rust in sorghum.the ability of the wheat lr34 multi-pathogen resistance gene (lr34res) to function across a wide taxonomic boundary was investigated in transgenic sorghum bicolor. increased resistance to sorghum rust and anthracnose disease symptoms following infection with the biotrophic pathogen puccinia purpurea and the hemibiotroph colletotrichum sublineolum respectively occurred in transgenic plants expressing the lr34res abc transporter. transgenic sorghum lines that highly expressed the wheat lr34res gen ...201728301718
patterns of light and nitrogen distribution in relation to whole canopy carbon gain in c3 and c4 mono- and dicotyledonous species.an analytical model was used to describe the optimal nitrogen distribution. from this model, it was hypothesized that the non-uniformity of the nitrogen distribution increases with the canopy extinction rate for light and the total amount of free nitrogen in the canopy, and that it is independent of the slope of the relation between light saturated photosynthesis (pm) and leaf nitrogen content (nl). these hypotheses were tested experimentally for plants with inherently different architectures an ...199528306967
the use of stable carbon isotope analysis in rooting studies.stable carbon isotope analysis was evaluated as a means of predicting the relative proportions of c3 and c4 root phytomass in species mixtures. the following mixtures of c3 and c4 species were used: 1) big bluestem (andropogon gerardii)/cheatgrass (bromus tectorum), 2) little bluestem (schizachyrium scoparium)/cheatgrass, and 3) sorghum (sorghum bicolor)/sunflower (helianthus annuus). there was a significant correlation (p<0.01) between % c4 phytomass and stable carbon isotope values for each of ...198528311309
land-use change in indian tropical agro-ecosystems: eco-energy estimation for socio-ecological sustainability.this study was carried out to understand the ecological and economic sustainability of floriculture and other main crops in indian agro-ecosystems. the cultivation practices of four major flower crops, namely jasminum multiflorum, crossandra infundibuliformis, chrysanthemum and tagetes erecta, were studied in detail. the production cost of flowers in terms of energy was calculated to be 99,622-135,996 compared to 27,681-69,133 mj ha(-1) for the main crops, namely oryza sativa, eleusine coracana, ...201728315232
production of bioherbicide by phoma sp. in a stirred-tank bioreactor.the objective of this work was to produce an herbicide by submerged fermentation in a stirred-tank bioreactor and to assess the potential herbicidal in pre-emergence, post-emergence, and in a detached leaves of cucumis sativus var species. wisconsin (cucumber) and sorghum bicolor (sorghum) species. fermentations were carried out in a stirred-tank bioreactor with useful volume of 3l. stirring rate (40, 50, and 60 rpm) and aeration (1, 2 and 3 vvm) were the variables studied for bioherbicide produ ...201628330302
the draft genome sequence of the plant growth promoting rhizospheric bacterium enterobacter cloacae sbp-8.here, we report the draft genome sequence and annotation of plant growth promoting rhizobacterium enterobacter cloacae sbp-8 isolated from the rhizosphere of sorghum bicolor l. growing in desert region of rajasthan, india. from the genome sequences, we identified the genes encoding plant growth promoting properties such as 1-aminocyclopropane-1-carboxylate deaminase (acds), phosphate solubilisation, siderophore, and iaa (indole acetic acid) production. the genes encoding different functions requ ...201728386532
population and function analysis of cultivable bacteria associated with spores of arbuscular mycorrhizal fungus gigaspora margarita.this study was aimed to investigate the diversity and function of bacterial population associated with gigaspora margarita spores. the fungus was propagated in sterilized sand/soil pots using alfalfa (medicago sativa), grain sorghum (sorghum bicolor), or maize (zea mays) as host plants, or in sterilized vermiculite pots using alfalfa as host plants, respectively. bacteria were isolated from the new-formed spores using diluted plate method, and typical bacterial isolates were identified according ...201728391471
overexpression of sbap37 in rice alleviates concurrent imposition of combination stresses and modulates different sets of leaf protein profiles.sbap37 transcription factor contributes to a combination of abiotic stresses when applied simultaneously in rice. it modulates a plethora of proteins that might regulate the downstream pathways to impart salt stress tolerance. apetala type of transcription factor was isolated from sorghum bicolor (sbap37), overexpressed in rice using a salt inducible abscisic acid 2 (aba2) promoter through agrobacterium tumefaciens following in planta method. transgenics were confirmed by pcr amplification of sb ...201728393269
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