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une-en iso/iec 17025:2005-accredited method for the determination of pesticide residues in fruit and vegetable samples by lc-ms/ms.a rapid, simple and sensitive multi-residue method was developed and validated for the simultaneous quantification and confirmation of 69 pesticides in fruit and vegetables using liquid chromatography-tandem mass spectrometry (lc-ms/ms). the samples were extracted following the quick, easy, cheap, effective, rugged and safe method known as quechers. mass spectrometric conditions were individually optimised for each analyte in order to achieve maximum sensitivity in multiple reaction monitoring ( ...201020730643
bacterial wilt resistance in tomato, pepper, and eggplant: genetic resources respond to diverse strains in the ralstonia solanacearum species complex.bacterial wilt, caused by strains belonging to the ralstonia solanacearum species complex, inflicts severe economic losses in many crops worldwide. host resistance remains the most effective control strategy against this disease. however, wilt resistance is often overcome due to the considerable variation among pathogen strains. to help breeders circumvent this problem, we assembled a worldwide collection of 30 accessions of tomato, eggplant and pepper (core-tep), most of which are commonly used ...201120795852
an in vivo rnai assay identifies major genetic and cellular requirements for primary pirna biogenesis in drosophila.in drosophila, piwi proteins and bound piwi-interacting rnas (pirnas) form the core of a small rna-mediated defense system against selfish genetic elements. within germline cells, pirnas are processed from pirna clusters and transposons to be loaded into piwi/aubergine/ago3 and a subset of pirnas undergoes target-dependent amplification. in contrast, gonadal somatic support cells express only piwi, lack signs of pirna amplification and exhibit primary pirna biogenesis from pirna clusters. neithe ...201020818334
vdsnf1, the sucrose nonfermenting protein kinase gene of verticillium dahliae, is required for virulence and expression of genes involved in cell-wall degradation.verticillium dahliae is a soilborne fungus causing vascular wilt in a diverse array of plant species. its virulence has been attributed, among other factors, to the activity of hydrolytic cell wall-degrading enzymes (cwde). the sucrose nonfermenting 1 gene (vdsnf1), which regulates catabolic repression, was disrupted in v. dahliae tomato race 1. expression of cwde in the resulting mutants was not induced in inductive medium and in simulated xylem fluid medium. growth of the mutants was significa ...201120839958
elective affinities: a tudor-aubergine tale of germline partnership.in drosophila melanogaster and many other metazoans, the specification of germ cells requires cytoplasmic inheritance of maternally synthesized rna and protein determinants, which are assembled in electron-dense cytoplasmic structures known as germ or polar granules, found at the posterior end of the oocytes. recent studies have shown that the formation of germ granules is dependent on the interaction of proteins containing tudor domains with the piwi-interacting rna (pirna)-binding piwi protein ...201020844011
potential of trap crops for integrated management of the tropical armyworm, spodoptera litura in tobacco.the tropical armyworm, spodoptera litura (f.) (lepidoptera: noctuidae), is an important pest of tobacco, nicotiana tabacum l. (solanales: solanaceae), in south china that is becoming increasingly resistant to pesticides. six potential trap crops were evaluated to control s. litura on tobacco. castor bean, ricinus communis l. (malpighiales: euphorbiaceae), and taro, colocasia esculenta (l.) schott (alismatales: araceae), hosted significantly more s. litura than peanut, arachis hypogaea l. (fabale ...201020874598
new 5-o-caffeoylquinic acid derivatives in fruit of the wild eggplant relative solanum viarum.fruit of the cultivated eggplant species solanum melongena, solanum aethiopicum, and solanum macrocarpon, and wild relatives including solanum anguivi and solanum incanum, have a high content of hydroxycinnamic acid conjugates with potential human health benefits. typically, caffeoylquinic acid esters predominate, and in particular 5-o-(e)-caffeoylquinic acid. by contrast, fruit from accession pi 319855 in the usda eggplant core collection, unambiguously identified as solanum viarum by morpholog ...201020886887
aubergine is a component of a nanos mrna localization complex.localization of nanos (nos) mrna to the posterior pole of the drosophila oocyte is essential for abdominal segmentation and germline development during embryogenesis. posterior localization is mediated by a complex cis-acting localization signal in the nos 3' untranslated region that comprises multiple partially redundant elements. genetic analysis suggests that this signal is recognized by rna-binding proteins and associated factors that package nos mrna into a localization competent ribonucleo ...201120937269
maternal impairment of transposon regulation in drosophila melanogaster by mutations in the genes aubergine, piwi and suppressor of variegation 205.tp5, a p element inserted in the telomere-associated sequences of the x chromosome, represses the excision of other p elements in the germ line through a combination of maternal and zygotic effects. the maternal component of this repression is impaired by heterozygous mutations in the aubergine and suppressor of variegation 205 genes; one mutation in the piwi gene also appears to impair repression. in the female germ line, the level of tp5 mrna is increased by these impairing mutations. the impa ...201020943007
novel role of specific tudor domains in tudor-aubergine protein complex assembly and distribution during drosophila oogenesis.germ cells give rise to the next generation and contain ribonucleoprotein particles, germ granules. in these granules, piwi protein aubergine has been shown to interact with tudor protein in drosophila. tudor protein has 11 tudor domains and it has been unclear to what extent all these domains are involved in the interaction with aubergine. here we present direct biochemical evidence that tudor-aubergine interaction surface is composed of different tudor domains including those that have not bee ...201020946872
maternal mrna deadenylation and decay by the pirna pathway in the early drosophila embryo.piwi-associated rnas (pirnas), a specific class of 24- to 30-nucleotide-long rnas produced by the piwi-type of argonaute proteins, have a specific germline function in repressing transposable elements. this repression is thought to involve heterochromatin formation and transcriptional and post-transcriptional silencing. the pirna pathway has other essential functions in germline stem cell maintenance and in maintaining germline dna integrity. here we uncover an unexpected function of the pirna p ...201020953170
reversible male sterility in eggplant (solanum melongena l.) by artificial microrna-mediated silencing of general transcription factor genes.since decades, plant male sterility is considered a powerful tool for biological containment to minimize unwanted self-pollination for hybrid seed production. furthermore, prevention of pollen dispersal also answers to concerns regarding transgene flow via pollen from genetically modified (gm) crops to traditional crop fields or wild relatives. we induced male sterility by suppressing endogenous general transcription factor genes, tafs, using anther-specific promoters combined with artificial mi ...201120955179
segregation distortion and linkage analysis in eggplant (solanum melongena l.).an anther-derived doubled haploid (dh) population and an f2 mapping population were developed from an intraspecific hybrid between the eggplant breeding lines 305e40 and 67/3. the former incorporates an introgressed segment from solanum aethiopicum gilo group carrying the gene rfo-sa1, which confers resistance to fusarium oxysporum; the latter is a selection from an intraspecific cross involving two conventional eggplant varieties and lacks rfo-sa1. initially, 28 aflp primer combinations (pcs) w ...201020962887
evaluation of copper-induced stress on eggplant (solanum melongena l.) seedlings at the molecular and population levels by use of various biomarkers.heavy-metal contamination is an important environmental problem in the world. it is known that high concentrations of heavy metals cause toxic damage to cells and tissues. in this study the effects of copper (cu(2+)) contamination were determined at the molecular and population levels in eggplant (solanum melongena l.) seedlings exposed to various concentrations of the metal ion. inhibition of root growth, reduction in dry weight and total soluble protein content in the roots of eggplant seedlin ...201120970520
biogenesis pathways of pirnas loaded onto ago3 in the drosophila testis.piwi-interacting rnas (pirnas) silence transposable elements in animal germ cells. in drosophila ovaries, pirnas are produced by two distinct pathways: the "ping-pong" amplification cycle that operates in germ cells and a ping-pong-independent pathway termed the primary pathway that mainly operates in somatic cells. ago3, one of three piwi proteins in flies, is involved in the ping-pong cycle in ovaries. we characterized ago3-associated pirnas in fly testes and found that like in ovaries, ago3 f ...201020980675
trans-cleaving hammerhead ribozymes with tertiary stabilizing motifs: in vitro and in vivo activity against a structured viroid rna.trans-cleaving hammerheads with discontinuous or extended stem i and with tertiary stabilizing motifs (tsms) have been tested previously against short rna substrates in vitro at low mg(2+) concentration. however, the potential of these ribozymes for targeting longer and structured rnas in vitro and in vivo has not been examined. here, we report the in vitro cleavage of short rnas and of a 464-nt highly structured rna from potato spindle tuber viroid (pstvd) by hammerheads with discontinuous and ...201021097888
identification of two conserved cis-acting elements, mycs and p1bs, involved in the regulation of mycorrhiza-activated phosphate transporters in eudicot species.• in this study, six putative promoter regions of phosphate transporter pht1;3, pht1;4 and pht1;5 genes were isolated from eggplant and tobacco using the inverse polymerase chain reaction (ipcr). the isolated sequences show evolutionary conservation and divergence within/between the two groups of pht1;3 and pht1;4/pht1;5. • histochemical analyses showed that all six promoter fragments were sufficient to drive β-glucuronidase (gus) expression specifically in arbuscular mycorrhizal (am) tobacco ro ...201021106037
targeting and anchoring tudor in the pole plasm of the drosophila oocyte.germline formation is a highly regulated process in all organisms. in drosophila embryos germ cells are specified by the pole plasm, a specialized cytoplasmic region containing polar granules. components of these granules are also present in the perinuclear ring surrounding nurse cells, the nuage. two such molecules are the vasa and tudor proteins. how tudor localizes and is maintained in the pole plasm is, however, not known.201021179512
a role for vasa in regulating mitotic chromosome condensation in drosophila.vasa (vas) is a conserved dead-box rna helicase expressed in germline cells that localizes to a characteristic perinuclear structure called nuage. previous studies have shown that vas has diverse functions, with roles in regulating mrna translation, germline differentiation, pole plasm assembly, and piwi-interacting rna (pirna)-mediated transposon silencing. although vas has also been implicated in the regulation of germline proliferation in drosophila and mice, little is known about whether vas ...201021185189
ectopic expression of germline genes drives malignant brain tumor growth in drosophila.model organisms such as the fruit fly drosophila melanogaster can help to elucidate the molecular basis of complex diseases such as cancer. mutations in the drosophila gene lethal (3) malignant brain tumor cause malignant growth in the larval brain. here we show that l(3)mbt tumors exhibited a soma-to-germline transformation through the ectopic expression of genes normally required for germline stemness, fitness, or longevity. orthologs of some of these genes were also expressed in human somatic ...201021205669
tea-industry waste activated carbon, as a novel adsorbent, for separation, preconcentration and speciation of chromium.activated carbon was produced from tea-industry wastes (tiwac) and employed as a low cost and effective solid phase material for the separation, preconcentration and speciation of chromium species without using a complexing agent, prior to determination by flame atomic absorption spectrometry (faas). the characterization of tiwac was performed by utilizing several techniques such as fourier transform infrared (ftir) spectroscopy, scanning electron microscopy (sem), and elemental analysis. the ad ...201021296208
immunochromatography for the rapid determination of cadmium concentrations in wheat grain and eggplant.a simple and quick on-site test for trace levels of cadmium (cd) in food is needed because of the human toxicity of this heavy metal. we developed an immunochromatography kit which uses the antigen-antibody complex reaction between the cd-ethylenediaminetetraacetic acid (cd-edta) complex and an anti-cd-edta antibody. we previously reported the successful use of this kit to determine cd concentrations in brown rice with respect to the international standard: 0.4 mg kg?¹. here, we measured, using ...201121337581
analysis of moisture desorption isotherms of eggplant (solanum melongena).sorption isotherms of eggplant were determined employing, as experimental technique, a static gravimetric method, using saturated salt solutions to achieve the equilibrium. the experiments were carried out at different temperatures (20, 35, 50 and 65 °c). the sorption isotherms can be classified, according to brunauer's classification, as type ii or iii depending on temperature. equilibrium moisture content data were correlated by two models usually applied to foodstuffs (brunauer--emmet--teller ...201021339160
non-coding rnas enter mitosis: functions, conservation and implications.nuage (or commonly known as chromatoid body in mammals) is a conserved germline-specific organelle that has been linked to the piwi-interacting rna (pirna) pathway. pirnas are a class of gonadal-specific rnas that are ~23-29 nucleotides in length and protect genome stability by repressing the expression of deleterious retrotransposons. more recent studies in drosophila have implicated the pirna pathway in other functions including canalization of embryonic development, regulation of maternal gen ...201121356070
tomato (solanum lycopersicum): a model fruit-bearing crop.introductiontomato (solanum lycopersicum) is one of the most important vegetable plants in the world. it originated in western south america, and domestication is thought to have occurred in central america. because of its importance as food, tomato has been bred to improve productivity, fruit quality, and resistance to biotic and abiotic stresses. tomato has been widely used not only as food, but also as research material. the tomato plant has many interesting features such as fleshy fruit, a s ...200821356708
dissipation of flubendiamide in/on brinjal (solanum melongena) fruits.a field experiment was conducted at anand agricultural university, anand during sept-dec, 2009 to study the rate of degradation of flubendiamide in/on brinjal fruits following foliar application of fame 480 sc at 90 (standard dose) and 180 (double dose) g a.i. ha( -1). the residues estimated using hplc revealed persistence of flubendiamide in/on brinjal till 3rd and 7th day after the last spray at standard and double dose, respectively. the residues of flubendiamamde were reported as parent comp ...201121360001
determination of free inositols and other low molecular weight carbohydrates in vegetables.different low molecular weight carbohydrates including saccharides, polyalcohols, sugar acids, and glycosides have been identified and quantified in different edible vegetables from asteraceae, amarantaceae, amarylidaceae, brassicaceae, dioscoreaceae, and solanaceae families by gas chromatography-mass spectrometry. apart from glucose, fructose, and sucrose, other saccharides such as sedoheptulose in chicory, spinach, cabbage, purple yam, eggplant, radish, and oak leaf lettuce, rutinose in eggpla ...201121366313
trapping whiteflies? a comparison of greenhouse whitefly (trialeurodes vaporariorum) responses to trap crops and yellow sticky traps.greenhouse whiteflies, trialeurodes vaporariorum (westwood), are notorious pests that, through the reduction of crop yields and excretion of honeydew, cause significant economic losses for sweet pepper (capsicum annuum l.) growers. chemical and biological controls are the two most common forms of whitefly management in greenhouses. consequently, insecticide resistance and inadequate control have rejuvenated interest in alternative tactics. in the present study, whitefly responses to trap crops ( ...201121394873
bioactivities of glycoalkaloids and their aglycones from solanum species.potatoes, tomatoes, and aubergines are all species of the solanum genus and contain a vast array of secondary metabolites including calystegine alkaloids, phenolic compounds, lectins, and glycoalkaloids. glycoalkaloids have been the subject of many literature papers, occur widely in the human diet, and are known to induce toxicity. therefore, from a food safety perspective further information is required regarding their analysis, toxicity, and bioavailability. this is especially important in cro ...201121401040
the egg-plant (solánum melongena l.) in decholesterolization. 194521434243
papi, a novel tudor-domain protein, complexes with ago3, me31b and tral in the nuage to silence transposition.the nuage is a germline-specific perinuclear structure that remains functionally elusive. recently, the nuage in drosophila was shown to contain two of the three piwi proteins - aubergine and argonaute 3 (ago3) - that are essential for germline development. the piwi proteins bind to piwi-interacting rnas (pirnas) and function in epigenetic regulation and transposon control. here, we report a novel nuage component, papi (partner of piwis), that contains a tudor domain and interacts with all three ...201121447556
populations of predators and parasitoids of bemisia tabaci (hemiptera: aleyrodidae) after the application of eight biorational insecticides in vegetable crops.the sweetpotato whitefly, bemisia tabaci (gennadius) (hemiptera: aleyrodidae), is an important pest of vegetables and many other crops worldwide. eight biorational insecticides (based on oil, plant derivatives, insect growth regulator and fungus) were evaluated in the field for their influence on populations of six natural enemies of b. tabaci. natural populations of two predators [chrysoperla carnea stephen (neuroptera: chrysopidae) and orius spp. (hemiptera: anthocoridae)] and two genera of pa ...201121480463
protein contact dermatitis: review of 27 cases.background: protein contact dermatitis (pcd) is a rare and underdiagnosed condition that many dermatologists fail to recognize. nevertheless, increasing awareness of the condition and the substances responsible has led to a rise in the number of published cases in recent years. objective: to determine the clinical characteristics and allergens implicated in pcd in our setting. material and methods: a retrospective observational study of all patients diagnosed with pcd in the last 10years was und ...201121497331
the effect of host plants on tetranychus evansi, tetranychus urticae (acari: tetranychidae) and on their fungal pathogen neozygites floridana (entomophthorales: neozygitaceae).in a series of tritrophic-level interaction experiments, the effect of selected host plants of the spider mites, tetranychus evansi and tetranychus urticae, on neozygites floridana was studied by evaluating the attachment of capilliconidia, presence of hyphal bodies in the infected mites, mortality from fungal infection, mummification and sporulation from fungus-killed mite cadavers. host plants tested for t. evansi were tomato, cherry tomato, eggplant, nightshade, and pepper while host plants t ...201121510956
[relationship between lead levels in colostrum, dietary intake, and socioeconomic characteristics of puerperal women in goiânia, brazil.]objective: to estimate the levels of lead in human colostrum and investigate whether they are correlated with dietary intake and socioeconomic factors in puerperal women. method: this cross-sectional study was carried out with 80 puerperal women from a maternity hospital in goiânia, brazil. data were collected between july and december 2008. a questionnaire was used to collect socioeconomic and lifestyle information. a dietary intake frequency questionnaire was also applied. after that, colostru ...201121603767
restoring pollen fertility in transgenic male-sterile eggplant by cre/loxp-mediated site-specific recombination system.this study was designed to control plant fertility by cell lethal gene barnase expressing at specific developmental stage and in specific tissue of male organ under the control of cre/loxp system, for heterosis breeding, producing hybrid seed of eggplant. the barnase-coding region was flanked by loxp recognition sites for cre-recombinase. the eggplant inbred/pure line ('e-38') was transformed with cre gene and the inbred/pure line ('e-8') was transformed with the barnase gene situated between lo ...201021637486
a four-gene study of evolutionary relationships in solanum section acanthophora.the "spiny solanums," solanum subgenus leptostemonum (solanaceae), comprise a large lineage with over 350 species and include the cultivated eggplant, solanum melongena. despite the importance of this subgenus, phylogenetic relationships among these taxa are currently unclear. the present research contributes to this understanding while focusing on solanum section acanthophora, a group of ca. 19 species defined by the presence of simple hairs, rather than the stellate hairs common across the res ...200521652438
identification of snp and ssr markers in eggplant using rad tag sequencing.the eggplant (solanum melongena l.) genome is relatively unexplored, especially compared to those of the other major solanaceae crops tomato and potato. in particular, no snp markers are publicly available; on the other hand, over 1,000 ssr markers were developed and publicly available. we have combined the recently developed restriction-site associated dna (rad) approach with illumina dna sequencing for rapid and mass discovery of both snp and ssr markers for eggplant.201121663628
molecular characterization and infectivity of a tomato leaf curl new delhi virus variant associated with newly emerging yellow mosaic disease of eggplant in india.begomoviruses have emerged as serious problem for vegetable and fiber crops in the recent past, frequently in tropical and subtropical region of the world. the association of begomovirus with eggplant yellow mosaic disease is hitherto unknown apart from one report from thailand. a survey in nagpur, central india, in 2009-2010 showed severe incidence of eggplant yellow mosaic disease. here, we have identified and characterized a begomovirus responsible for the newly emerging yellow mosaic disease ...201121676270
molecular diversity analysis of eggplant (solanum melongena) genetic resources.eggplant (solanum melongena), a vegetable that is cultivated worldwide, is of considerable importance to agriculture in china. we analyzed the diversity of this plant using inter-simple sequence repeat (issr) and rapd procedures to subdivide 143 chinese-cultivated eggplants based on coefficient of parentage, genetic diversity index (gdi) and canonical discriminant analysis. issr markers were more effective than rapd markers for detecting genetic diversity, which ranged from 0.10-0.51, slightly l ...201121710466
dead-box rna helicase belle/ddx3 and the rna interference pathway promote mitotic chromosome segregation.during mitosis, faithful inheritance of genetic material is achieved by chromosome segregation, as mediated by the condensin i and ii complexes. failed chromosome segregation can result in neoplasm formation, infertility, and birth defects. recently, the germ-line-specific dead-box rna helicase vasa was demonstrated to promote mitotic chromosome segregation in drosophila by facilitating robust chromosomal localization of barren (barr), a condensin i component. this mitotic function of vasa is me ...201121730191
two new antioxidant malonated caffeoylquinic acid isomers in fruits of wild eggplant relatives.fruits of the cultivated eggplant species <i>solanum melongena</i> and its wild relative <i>solanum incanum</i> have a high content of hydroxycinnamic acid conjugates, which are implicated in the human health benefits of various fruits and vegetables. monocaffeoylquinic acid esters, in particular 5-<i>o</i>-(-<i>e</i>)-caffeoylquinic acid, are usually predominant in solanaceous fruits and tubers. two closely related caffeoylquinic acid derivatives with longer c<sub>18</sub>-hplc retention times ...201121800866
radioactivity and heavy metal concentrations in food samples from rize, turkey.background: rize in turkey was contaminated by the chernobyl accident in 1986. a comprehensive study was planned and carried out to determine the radioactivity levels and heavy metal concentrations in four food categories collected in rize in 2008, 2009 and 2010. results: tomato showed the highest concentration of (238) u, at 9.43 ± 0.128 bq kg(-1) , whereas the lowest concentration of 0.20 ± 0.02 bq kg(-1) was measured in aubergine samples. the highest concentration of (232) th was measured at ...201121815162
phenylethyl cinnamides as potential alpha-glucosidase inhibitors from the roots of solanum melongena.bioassay-guided fractionation against alpha-glucosidase resulted in isolation and identification of six phenolic compounds (1-6) from the 70% etoh extract of the roots of solanum melongena l. (solanaceae). of these, three phenylethyl cinnamides, n-trans-feruloyl tyramine (1), n-trans-p-coumaroyl tyramine (2) and n-cis-p-coumaroyl tyramine (3) possessed inhibitory activity against alpha-glucosidase with ic50 values of 500.6, 5.3 and 46.3 microm, respectively. mechanism analysis revealed these phe ...201121815424
vreteno, a gonad-specific protein, is essential for germline development and primary pirna biogenesis in drosophila.in drosophila, piwi proteins associate with piwi-interacting rnas (pirnas) and protect the germline genome by silencing mobile genetic elements. this defense system acts in germline and gonadal somatic tissue to preserve germline development. genetic control for these silencing pathways varies greatly between tissues of the gonad. here, we identified vreteno (vret), a novel gonad-specific protein essential for germline development. vret is required for pirna-based transposon regulation in both g ...201121831924
genetic variability and evolutionary analyses of the coat protein gene of tomato mosaic virus.tomato mosaic virus (tomv), a member of the genus tobamovirus, infects several ornamental and horticultural crops worldwide. in this study, the nucleotide sequences of the coat protein gene of worldwide tomv isolates were analyzed to estimate the genetic structure and diversity of this virus and the involved evolutionary forces. the phylogenetic analysis showed three clades with high bootstrap support: clade i contained three tomv isolates from brazil collected from pepper, clade ii comprised on ...201121881940
Expression of a cloned sweet potato catalase SPCAT1 alleviates ethephon-mediated leaf senescence and H(2)O(2) elevation.In this report a full-length cDNA, SPCAT1, was isolated from ethephon-treated mature L3 leaves of sweet potato. SPCAT1 contained 1479 nucleotides (492 amino acids) in its open reading frame, and exhibited high amino acid sequence identities (ca. 71.2-80.9%) with several plant catalases, including Arabidopsis, eggplant, grey mangrove, pea, potato, tobacco and tomato. Gene structural analysis showed that SPCAT1 encoded a catalase and contained a putative conserved internal peroxisomal targeting si ...201221893366
cardioprotective properties of raw and cooked eggplant (solanum melongena l).although eggplants are known to be part of a healthy diet, the effects of this fruit on cardioprotection are not known. the present study examined the role of raw and grilled eggplants on cardioprotection using an isolated perfusion heart model. the animals were fed freeze-dried products of either raw or grilled eggplants for 30 days. after 30 days, isolated working hearts were subjected to 30 min ischemia followed by 2 h of reperfusion. left ventricular function was monitored, and myocardial in ...201121894326
Functional diversification of the GALA type III effector family contributes to Ralstonia solanacearum adaptation on different plant hosts.Type III effectors from phytopathogenic bacteria exhibit a high degree of functional redundancy, hampering the evaluation of their precise contribution to pathogenicity. This is illustrated by the GALA type III effectors from Ralstonia solanacearum, which have been shown to be collectively, but not individually, required for disease on Arabidopsis thaliana and tomato. We investigated evolution, redundancy and diversification of this family in order to understand the individual contribution of th ...201121902695
dissipation pattern of flubendiamide in/on brinjal (solanum melongena l.).residues of flubendiamide and desiodo flubendiamide were studied following three applications of flubendiamide 480 sc at 7 days interval at 90 and 180 g a.i. ha(-1) in/on brinjal fruits. an average initial deposit of 0.33 and 0.61 mg kg(-1) of flubendiamide was observed respectively after application at single and double dosages. the residues of flubendiamide dissipated quickly at both the dosages, and after 3 days, the extent of dissipation was found to be about 76% and 79% at the single and do ...201221927786
study and characterization of polyphenol oxidase from eggplant (solanum melongena l.).in this study the catecholase and cresolase activities of eggplant polyphenol oxidase (ppo) were investigated. enzyme activity was determined by measuring the increase in absorbance using catechol as substrate and 3-methyl-2-benzothiazolinone hydrazone (mbth) as coupled reagent. the effects of substrate specificity, heat inactivation, temperature, ph, and inhibitors were investigated to understand the enzymatic alteration of ready-to-eat preparations. browning of vegetables was determined throug ...201121942648
eggplant polyphenol oxidase multigene family: cloning, phylogeny, expression analyses and immunolocalization in response to wounding.though polyphenol oxidase (ppo) genes from tomato and potato have been extensively studied, information about ppo genes in eggplant (solanum melongena) is lacking. the main objective of this study is to understand the structural and functional aspects of eggplant ppo genes. six eggplant ppo genes (smeppo1-6) cloned by race and genome walking were found to be intronless and correspond to eight eggplant unigenes. comprehensive sequence analyses indicated that the eggplant ppo genes exhibit conside ...201121945722
leaf cuticular n-alkanes as markers in the chemotaxonomy of the eggplant (solanum melongena l.) and related species.the complex of species formed by eggplant (solanum melongena l.) and its wild and weedy relatives (mainly s. incanum l. and s. insanum l.) is characterised by an extreme morphological divergence that is not always associated with genetic variation. the taxonomy of so-called 'spiny solanum' species (subgenus leptostemonum) is therefore extremely unclear. cultivated eggplant lacks resistance to pests that frequently occur among the wild forms and species. as these wild plants are a potential gene ...201121973213
the oomycete broad-host-range pathogen phytophthora capsici.phytophthora capsici is a highly dynamic and destructive pathogen of vegetables. it attacks all cucurbits, pepper, tomato and eggplant, and, more recently, snap and lima beans. the disease incidence and severity have increased significantly in recent decades and the molecular resources to study this pathogen are growing and now include a reference genome. at the population level, the epidemiology varies according to the geographical location, with populations in south america dominated by clonal ...201222013895
[bioinformatic prediction of conserved micrornas and their target genes in eggplant (solanum melongena l.)].micrornas (mirnas), a recently discovered class of small (-21nt), non-coding, endogenous, single-stranded rnas in eukaryotes, regulate gene expression negatively at the post-transcriptional levels depending on the extent of complementation between mirna and mrna. to date, a large number of mirnas have been reported in many species, but none for eggplant (solanum melongena l.). in this paper, a computational homology search approach based on the conservation of mirna sequences and the stem-loop h ...201122049693
eggplant mild leaf mottle virus (emlmv), a new putative member of the genus ipomovirus that harbors an hc-pro gene.since 2003, a new viral disease of eggplant (solanum melongena l.) has been spreading in fields in the jordan and arava valleys, israel. the symptoms of this disease include mild leaf mottling and varying degrees of fruit distortion. this disease can be transmitted by mechanical sap inoculation, as well as by the whitefly bemisia tabaci (homoptera, aleyrodidae) and has been tentatively named eggplant mild leaf mottle virus (emlmv). our study aimed to determine the complete sequence and genome or ...201222086504
heterotypic pirna ping-pong requires qin, a protein with both e3 ligase and tudor domains.pirnas guide piwi proteins to silence transposons in animal germ cells. reciprocal cycles of pirna-directed rna cleavage--catalyzed by the piwi proteins aubergine (aub) and argonaute3 (ago3) in drosophila melanogaster--expand the population of antisense pirnas in response to transposon expression, a process called the ping-pong cycle. heterotypic ping-pong between aub and ago3 ensures that antisense pirnas predominate. we show that qin, a pirna pathway gene whose protein product contains both e3 ...201122099305
drosophila piwi functions downstream of pirna production mediating a chromatin-based transposon silencing mechanism in female germ line.transposon control is a critical process during reproduction. the piwi family proteins can play a key role, using a pirna-mediated slicing mechanism to suppress transposon activity posttranscriptionally. in drosophila melanogaster, piwi is predominantly localized in the nucleus and has been implicated in heterochromatin formation. here, we use female germ-line-specific depletion to study piwi function. this depletion of piwi leads to infertility and to axis specification defects in the developin ...201122160707
virus-induced gene silencing in eggplant (solanum melongena).eggplant (solanum melongena) is an economically important vegetable requiring investigation into its various genomic functions. the current limitation in the investigation of genomic function in eggplant is the lack of effective tools available for conducting functional assays. virus-induced gene silencing (vigs) has played a critical role in the functional genetic analyses. in this paper, trv-mediated vigs was successfully elicited in eggplant. we first cloned the cds sequence of pds (phytoene ...201222268843
a new species of solanum (solanaceae) from south africa related to the cultivated eggplant.a new andromonoecious species related to the eggplant and belonging to solanum subgenus leptostemonum from southern africa is described. solanum umtuma voronts. & s.knapp, sp. nov. is found in the eastern part of south africa, and is sympatric with its close relative solanum linnaeanum hepper & p.m-l.jaeger. it is morphologically very similar to solanum cerasiferum dunal of northern tropical africa. a comparison table with similar and closely related species is provided, as are a distribution ma ...201222287927
development of selective markers linked to a major qtl for parthenocarpy in eggplant (solanum melongena l.).parthenocarpy, the ability to set fruits without pollination, is a useful trait for setting fruit under unfavorable conditions. to identify the loci controlling parthenocarpy in eggplant (solanum melongena l.), we constructed linkage maps by using co-dominant simple sequence repeat and single nucleotide polymorphism markers in f(2) populations derived from intraspecific crosses between two non-parthenocarpic lines (ls1934 and nakate-shinkuro) and a parthenocarpic line (ae-p03). total map distanc ...201222301906
gender-specific hierarchy in nuage localization of piwi-interacting rna factors in drosophila.piwi-interacting rnas (pirnas) are germline-specific small non-coding rnas that form pirna-induced silencing complexes (piriscs) by associating with piwi proteins, a subclade of the argonaute proteins predominantly expressed in the germline. piriscs protect the integrity of the germline genome from invasive transposable dna elements by silencing them. multiple pirna biogenesis factors have been identified in drosophila. the majority of pirna factors are localized in the nuage, electron-dense non ...201122303351
a chloroplastic rna ligase activity analogous to the bacterial and archaeal 2´-5' rna ligase.bacteria and archaea contain a 2'-5' rna ligase that seals in vitro 2',3'-cyclic phosphodiester and 5'-hydroxyl rna termini, generating a 2',5'-phosphodiester bond. in our search for an rna ligase able to circularize the monomeric linear replication intermediates of viroids belonging to the family avsunviroidae, which replicate in the chloroplast, we have identified in spinach (spinacea oleracea l.) chloroplasts a new rna ligase activity whose properties resemble those of the bacterial and archa ...201222336712
development of gene-based markers and construction of an integrated linkage map in eggplant by using solanum orthologous (sol) gene sets.we constructed an integrated dna marker linkage map of eggplant (solanum melongena l.) using dna marker segregation data sets obtained from two independent intraspecific f(2) populations. the linkage map consisted of 12 linkage groups and encompassed 1,285.5 cm in total. we mapped 952 dna markers, including 313 genomic ssr markers developed by random sequencing of simple sequence repeat (ssr)-enriched genomic libraries, and 623 single-nucleotide polymorphisms (snp) and insertion/deletion polymor ...201222350090
promoter analyses and transcriptional profiling of eggplant polyphenol oxidase 1 gene (smeppo1) reveal differential response to exogenous methyl jasmonate and salicylic acid.the transcriptional regulation of multigenic eggplant (solanum melongena) polyphenol oxidase genes (smeppo) is orchestrated by their corresponding promoters which mediate developmentally regulated expression in response to myriad biotic and abiotic factors. however, information on structural features of smeppo promoters and modulation of their expression by plant defense signals are lacking. in the present study, smeppopromoters were cloned by genome walking, and their transcription start sites ...201222377322
phylogeographic relationships among asian eggplants and new perspectives on eggplant domestication.the domestication history of eggplant (solanum melongena l.) has long been debated, with studies unable to narrow down where domestication occurred within a broad region of tropical asia. the most commonly hypothesized region is india, however china has an equally old written record of eggplant use dating ca. 2000 years before present. both regions have a high diversity of landraces and populations of putatively wild eggplant: solanum incanum l. in india and solanum undatum lam. in se asia. an a ...201222387533
homology-dependent silencing by an exogenous sequence in the drosophila germline.the study of p transposable element repression in drosophila melanogaster led to the discovery of the trans-silencing effect (tse), a homology-dependent repression mechanism by which a p-transgene inserted in subtelomeric heterochromatin (telomeric associated sequences) represses in trans, in the female germline, a homologous p-lacz transgene inserted in euchromatin. tse shows variegation in ovaries and displays a maternal effect as well as epigenetic transmission through meiosis. in addition, t ...201222413086
flow injection assessment of nitrate contents in fresh and cooked fruits and vegetables grown in fiji.nitrates form part of the essential chemistry of soils and plants. thus, plant roots are able to absorb nitrate directly from the soil. it has been discovered that human nitrate intake is mainly from vegetables. vegetables play an important role in human nutrition since they are an outstanding source for vitamins, minerals, and biologically active compounds. in risk assessment of nitrate contents, this study reports the nitrate levels of 8 commonly consumed fruits and vegetables grown in fiji, t ...201122417577
influence of helium-neon laser irradiation on seed germination in vitro and physico-biochemical characters in seedlings of brinjal (solanum melongena l.) var. mattu gulla.in the present study, the seeds of brinjal (solanum melongena l.) var. mattu gulla were irradiated with single exposure of he-ne laser at different doses of 5-40 j cm(-2) and germinated aseptically. thirty day old seedlings were harvested and the germination, growth, physiological and biochemical parameters were estimated and compared with un-irradiated control seedlings. a significant enhancement in growth characters were noted with respect to length, fresh and dry weight of shoots and roots. i ...201522515726
so near and yet so far: the specific case of ralstonia solanacearum populations from côte d'ivoire in africa.the genetic and phenotypic diversity of côte d'ivoire ralstonia solanacearum strains was assessed on a 168-strain collection sampled on solanaceae both in the southern lowlands and western highlands. phylotypes i, ii, and iii were prevalent, though at unexpected frequencies. phylotype i strains (87.5%) were genetically diverse and overrepresented in all agroecological areas, including highlands (aez iii). phylotype ii strains (10.7%) only belonged to one tropical lowland-adapted broad host range ...201222533876
genetically engineered vegetables expressing proteins from bacillus thuringiensis for insect resistance: successes, disappointments, challenges and ways to move forward.genetically engineered (ge) insect-resistant crops that express proteins from bacillus thuringiensis (bt) have been widely adopted in the two field crops currently commercially available, bt cotton and bt corn. however, the development and commercialization of bt vegetables has lagged in comparison, which is unfortunate since vegetables tend to be insecticide-intensive crops due to high pest pressure and cosmetic standards required for the market. while it is often stated that consumer choice ha ...201422538234
involvement of the chloroplastic isoform of trna ligase in the replication of viroids belonging to the family avsunviroidae.avocado sunblotch viroid, peach latent mosaic viroid, chrysanthemum chlorotic mottle viroid, and eggplant latent viroid (elvd), the four recognized members of the family avsunviroidae, replicate through the symmetric pathway of an rna-to-rna rolling-circle mechanism in chloroplasts of infected cells. viroid oligomeric transcripts of both polarities contain embedded hammerhead ribozymes that, during replication, mediate their self-cleavage to monomeric-length rnas with 5'-hydroxyl and 2',3'-phosp ...201222623792
relationships between the photochemical reflectance index (pri) and chlorophyll fluorescence parameters and plant pigment indices at different leaf growth stages.this study aimed to evaluate the photochemical reflectance index (pri) for assessing plant photosynthetic performance throughout the plant life cycle. the relationships between pri, chlorophyll fluorescence parameters, and leaf pigment indices in solanum melongena l. (aubergine; eggplant) were studied using photosynthetic induction curves both in short-term (diurnal) and long-term (seasonal) periods under different light intensities. we found good correlations between pri/non-photochemical quenc ...201222644476
sprout vacuum-infiltration: a simple and efficient agroinoculation method for virus-induced gene silencing in diverse solanaceous species.virus-induced gene silencing (vigs) is a robust technique for identifying the functions of plant genes. tobacco rattle virus (trv)-mediated vigs has been commonly used in many plants. in order to overcome the limitations of existing agroinoculation methods, we report an easy and effective method of agroinoculation for virus-induced gene silencing-sprout vacuum-infiltration (svi). using sprout vacuum-infiltration, we have successfully silenced the expression of phytoene desaturase and mg-protopor ...201222717672
first report of cucumber mosaic virus subgroups i and ii on soybean, pea, and eggplant in iran.cucumber mosaic virus (cmv) has the widest host range (> 1000 plant species) of any known plant virus, a large number of vector species, and a wide geographical distribution. a survey was conducted to determine the incidence of cmv of subgroups i and ii on selected host crops in northern iran. a total of 935 leaf samples from 10 host crops (tomato, pea, tobacco, soybean, watermelon, broad bean, squash, cucumber, eggplant, and lettuce) showing virus disease-like symptoms were collected in 12 citi ...201222720706
detection and identification of genetically modified ee-1 brinjal (solanum melongena) by single, multiplex and sybr® real-time pcr.brinjal is an important vegetable crop. major crop loss of brinjal is due to insect attack. insect-resistant ee-1 brinjal has been developed and is awaiting approval for commercial release. consumer health concerns and implementation of international labelling legislation demand reliable analytical detection methods for genetically modified (gm) varieties.201322729745
population dynamics of meloidogyne arenaria and pasteuria penetrans in a long-term crop rotation study.the endospore-forming bacterium pasteuria penetrans is an obligate parasite of root-knot nematodes (meloidogyne spp.). the primary objective of this study was to determine the effect of crop sequence on abundance of p. penetrans. the experiment was conducted from 2000 to 2008 at a field site naturally infested with both the bacterium and its host meloidogyne arenaria and included the following crop sequences: continuous peanut (arachis hypogaea) (p-p-p) and peanut rotated with either 2 years of ...200922736828
influence of plant combinations on population characteristics of bemisia tabaci biotypes b and q.in recent years, the dominant biotype of bemisia tabaci (gennadius) has shifted from biotype b to q in many locations of china. our field monitoring showed that b. tabaci biotype q could be found on pepper capsicum frutescens l. while biotype b could not be found on the plant. to study the role of the host plant in the displacement of biotype b by q, the adult mortality, female fecundity, and adult emergence of both biotypes b and q on different host combinations were studied using a model syste ...201222812132
solanum melongena: a potential source of antifungal agent.the antifungal activity of solanum melongena leaf, extracted with petroleum ether, chloroform, methanol and water was evaluated against three human pathogenic dermatophytes namely trichophyton mentagrophytes, t. rubrum and t. tonsurans and two opportunistic fungi candida albicans and trichosporon beigelii. maximum yield of plant components was 4.32 g, extracted in water and minimum 1.07 g in petroleum ether from 150 g of dry plant material. except water extract, all the extracts possessed signif ...201022815574
diversity and relationships of eggplants from three geographically distant secondary centers of diversity.eggplant (solanum melongena l.) was domesticated in the indo-birmanian region, which is also the primary center of diversity for this crop. from there eggplant spread to other regions, and diversity accumulated in several secondary centers of diversity. we have assessed the diversity and relationships of 52 accessions of eggplant from three geographically distant secondary centers of diversity (china, spain, and sri lanka) using 28 morphological descriptors and 12 highly polymorphic genomic ssrs ...201222848589
a rad tag derived marker based eggplant linkage map and the location of qtls determining anthocyanin pigmentation.both inter- and intra-specific maps have been developed in eggplant (solanum melongena l.). the former benefit from an enhanced frequency of marker polymorphism, but their relevance to marker-assisted crop breeding is limited. combining the restriction-site associated dna strategy with high throughput sequencing has facilitated the discovery of a large number of functional single nucleotide polymorphism (snp) markers discriminating between the two eggplant mapping population parental lines '305e ...201222912903
paramutation in drosophila linked to emergence of a pirna-producing locus.a paramutation is an epigenetic interaction between two alleles of a locus, through which one allele induces a heritable modification in the other allele without modifying the dna sequence. the paramutated allele itself becomes paramutagenic, that is, capable of epigenetically converting a new paramutable allele. here we describe a case of paramutation in animals showing long-term transmission over generations. we previously characterized a homology-dependent silencing mechanism referred to as t ...201222922650
genetic mapping of a major dominant gene for resistance to ralstonia solanacearum in eggplant.resistance of eggplant against ralstonia solanacearum phylotype i strains was assessed in a f(6) population of recombinant inbred lines (rils) derived from a intra-specific cross between s. melongena mm738 (susceptible) and ag91-25 (resistant). resistance traits were determined as disease score, percentage of wilted plants, and stem-based bacterial colonization index, as assessed in greenhouse experiments conducted in réunion island, france. the ag91-25 resistance was highly efficient toward str ...201322930132
pleurotus eryngii immobilized amberlite xad-16 as a solid-phase biosorbent for preconcentrations of cd2+ and co2+ and their determination by icp-oes.this article reports a method that is used for the preconcentration and determination of cd(2+) and co(2+) in vegetables, using pleurotus eryngii immobilized amberlite xad-16 as a solid-phase biosorbent. the concentrations of metals were determined by inductively coupled plasma-optical spectrometry (icp-oes). critical parameters, such as the ph of the solution, flow rate, the amount of biosorbent, type and volume of eluent, and the sample volume, that affect the solid-phase extraction (spe) proc ...201222967585
traffic-related trace element fate and uptake by plants cultivated in roadside soils in toronto, canada.this research examines traffic-related trace element emissions and their uptake by plants grown in urban roadside environments in toronto, canada. oregano (origanum vulgare), beets (beta vulgaris) and eggplants (solanum melongena) were cultivated at four locations with variable traffic-related metal inputs in 2010. the top 30 cm of soil at the medium-traffic location was first replaced with a triple mix soil (topsoil, compost and peat) to control for pre-existing contamination. the same soil was ...201323178768
accumulation of heavy metals in edible parts of vegetables irrigated with waste water and their daily intake to adults and children, district mardan, pakistan.green vegetable crops irrigated with wastewater are highly contaminated with heavy metals and are the main source of human exposure to the contaminants. in this study accumulation of eight heavy metals (cu, ni, zn, cr, fe, mn, co and pb) in green vegetables like allium cepa, allium sativum, solanum lycopersicum and solanum melongena, irrigated with wastewater in mardan are studied using atomic absorption spectrophotometer. the studied metals in vegetable grown on wastewater irrigated soil were s ...201323194556
nonomuraea solani sp. nov., an actinomycete isolated from eggplant root (solanum melongena l.).a novel actinomycete, designated strain neau-z6(t), was isolated from eggplant (solanum melongena l.) root. phylogenetic analysis based on the 16s rrna gene sequence showed that strain neau-z6(t) belonged to the genus nonomuraea, with highest sequence similarity to nonomuraea monospora pt 708(t) (98.83 %), nonomuraea rosea gw 12687(t) (98.55 %) and nonomuraea rhizophila yim 67092(t) (98.02 %). sequence similarities between strain neau-z6(t) and other species of the genus nonomuraea ranged from 9 ...201323203622
pharmacological reactivity of isolated guinea pig ileum to ethanol leaf extracts of amaranthus caudatus and solanum melongena.the pharmacological reactivity of guinea pig ileum to ethanol leaf extract of amaranthus caudatus and solanum melongena were determined in vitro. parameters evaluated include the threshold value and the concentration ratio (cr). the potency of the plant extracts as expressed by ec50, the emax (maximum response) and its corresponding concentration were determined from the concentration response curve in the absence or presence of 2x10-7 m atropine or 2x10-7 m mepyramine. the study showed that the ...201223235311
mutations to the pirna pathway component aubergine enhance meiotic drive of segregation distorter in drosophila melanogaster.diploid sexual reproduction involves segregation of allelic pairs, ensuring equal representation of genotypes in the gamete pool. some genes, however, are able to "cheat" the system by promoting their own transmission. the segregation distorter (sd) locus in drosophila melanogaster males is one of the best-studied examples of this type of phenomenon. in this system the presence of sd on one copy of chromosome 2 results in dysfunction of the non-sd-bearing (sd(+)) sperm and almost exclusive trans ...201323267055
clinico-immunological analysis of eggplant (solanum melongena) allergy indicates preponderance of allergens in the peel.: eggplant (solanum melongena l.) is known to cause food allergy in some asian countries but detailed studies on eggplant allergy are lacking.200923283148
function of piwi, a nuclear piwi/argonaute protein, is independent of its slicer activity.the piwi protein subfamily is essential for piwi-interacting rna (pirna) biogenesis, transposon silencing, and germ-line development, all of which have been proposed to require piwi endonuclease activity, as validated for two cytoplasmic piwi proteins in mice. however, recent evidence has led to questioning of the generality of this mechanism for the piwi members that reside in the nucleus. drosophila offers a distinct opportunity to study the function of nuclear piwi proteins because, among thr ...201323297219
maternal enhancement of cytotype regulation in drosophila melanogaster by genetic interactions between telomeric p elements and non-telomeric transgenic p elements.the x-linked telomeric p elements (tps) tp5 and tp6 regulate the activity of the entire p element family because they are inserted in a major locus for the production of piwi-interacting rnas (pirnas). the potential for this cytotype regulation is significantly strengthened when either tp5 or tp6 is combined with a non-telomeric x-linked or autosomal transgene that contains a p element. by themselves, none of the transgenic p elements have any regulatory ability. synergism between the telomeric ...201223374243
taxonomic notes on several wild relatives of solanum melongena l. (solanaceae): comments on.in the recent paper by meyer et al. (2012) some of the taxonomic assumptions relating to the closest wild relatives of solanum melongena l., the brinjal eggplant, are unsupported. this group is well-known for its taxonomic difficulties, therefore a consistent approach to the identification, nomenclature and species concepts of experimental plant material is essential to the fullest interpretation of the results of a genomic study such as theirs. effectively, meyer et al., treat several of the br ...201323380193
multiple roles for piwi in silencing drosophila transposons.silencing of transposons in the drosophila ovary relies on three piwi family proteins--piwi, aubergine (aub), and ago3--acting in concert with their small rna guides, the piwi-interacting rnas (pirnas). aub and ago3 are found in the germ cell cytoplasm, where they function in the ping-pong cycle to consume transposon mrnas. the nuclear piwi protein is required for transposon silencing in both germ and somatic follicle cells, yet the precise mechanisms by which piwi acts remain largely unclear. w ...201323392609
purification and characterisation of polyphenol oxidase (ppo) from eggplant (solanum melongena).eggplant (solanum melongena) is a very rich source of polyphenol oxidase (ppo), which negatively affects its quality upon cutting and postharvest processing due to enzymatic browning. ppo inhibitors, from natural or synthetic sources, are used to tackle this problem. one isoform of ppo was 259-fold purified using standard chromatographic procedures. the ppo was found to be a 112 kda homodimer. the enzyme showed very low k(m) (0.34 mm) and high catalytic efficiency (3.3×10(6)) with 4-methyl catec ...201223442630
wild relatives of the eggplant (solanum melongena l.: solanaceae): new understanding of species names in a complex group.the common or brinjal eggplant (solanum melongena l.) belongs to the leptostemonum clade (the "spiny" solanums) of the species-rich genus solanum (solanaceae). unlike most of the genus, the eggplant and its relatives are from the old world; most eggplant wild relatives are from africa. an informal system for naming eggplant wild relatives largely based on crossing and other biosystematics data has been in use for approximately a decade. this system recognises several forms of two broadly conceiv ...201323451138
persistence of thiacloprid residues on brinjal (solanum melongena l.).thiacloprid, a neonicotinoid insecticide with novel mode of action, is found to be effective against several lepidopteran as well as hemipteran pests. the present studies were carried out to observe the persistence pattern of thiacloprid on brinjal and to suggest suitable waiting period for the safety of consumers. persistence of thiacloprid in brinjal was studied following three applications of thiacloprid (alanto 240 sc) at 180 and 360 g a.i. ha(-1) at 7 days interval. residues of thiacloprid ...201323456274
a molecular marker for in situ genetic resource conservation of capsicum annuum var. acuminatum (solanaceae).the thailand cultivar pepper 'phrik man bangchang' (capsicum annuum var. acuminatum, solanaceae) was originally cultivated in the bangchang subdistrict, amphawa district in samut songkhram province. the cultivated areas are limited; we verified its distribution in thailand for in situ 'phrik man bangchang' genetic resource conservation. samples were collected from the original cultivation area of bangchang subdistrict (or) and were randomly explored in ratchaburi province (rb), khon kaen provinc ...201323479155
a description of the lactic acid bacteria microbiota associated with the production of traditional fermented vegetables in vietnam.an important part of the daily nourishment in vietnam constitutes of fermented vegetables. bacteria and especially lactic acid bacteria play a central role in the production of many fermented vegetables. the current study was conducted to investigate the diversity of native lactic acid bacteria (lab) populations in 'dua muoi' (mustard and beet fermentation) and 'ca muoi' (eggplant fermentation), three types of popular traditional fermented vegetables of vietnamese origin. to this end a polyphasi ...201323500611
production of interspecific hybrids between commercial cultivars of the eggplant (solanum melongena l.) and its wild relative s. torvum.interspecific hybrids between cultivars of eggplant (solanum melongena l.) and its wild relative s. torvum, which has disease resistance and desirable traits for crop improvement, were obtained by cross-hybridization and embryo rescue. twenty-one hybrid progenies were obtained and examined based on morphological traits, rapd and issr markers. five of them were confirmed to be true interspecific hybrids. eighteen and 14 bands from 7 rapd and 14 issr primers, respectively, were polymorphic and pre ...201323546959
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