Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| surface movements during the spreading of blood platelets. | when human blood platelets spread on a substratum they increase their surface area as much as 4-fold. we investigated the mechanism of spreading by light microscopy and by scanning and transmission electron microscopy. contact of a platelet with a glass surface induces formation of thin extensions which spread out over the substratum. these extensions resemble the actin-containing microspikes and lammelipodia of tissue cells in culture and appear to be drawn from the peripheral cortical layer as ... | 1988 | 3169030 |
| altered expression of platelet surface glycoproteins during storage. | human blood platelets were stored in autologous plasma at 4 degrees c or 22 degrees c and their surface changes were probed with three lectins--wheat germ agglutinin, lentil lectin and concanavalin a. platelets stored at either temperature for different times showed increased sensitivity to lectins. lectins which were nonagglutinating to fresh platelets readily agglutinated stored platelets. the platelets stored for 24 h or longer lost their ability to respond to thrombin but demonstrated enhanc ... | 1988 | 3179230 |
| biochemical properties of a 24 kilodalton membrane glycoprotein antigen complex from schistosoma mansoni. | antibodies were affinity purified from crude antiserum by elution from the 24 kda region of preparative one-dimensional western blots containing immobilized adult schistosoma mansoni inner bilayer membrane proteins. they were shown to be specific for a single acidic polypeptide complex, smgp24, following immunoblotting from two-dimensional polyacrylamide gels. these antibodies were then used to detect the presence of the smgp24 complex in fractions prepared from lectin affinity chromatography, p ... | 1988 | 3185620 |
| disulfide-linked receptors for mitogenic lectins on piglet lymphocytes. | the interaction between leucoagglutinating phytohemagglutinin (l-pha), concanavalin a (con a), soybean agglutinin (sba) and lentil lectin (lch) with disulfide-linked cell surface receptors on lymphocytes from mesenteric lymph nodes of 3-day piglets (pmln) was investigated. surface radioiodinated pmln lymphocytes were lysed with buffer containing nonidet-p40. the lysates were adsorbed on lectin-agarose derivatives (or bovine serum albumin-agarose). eluates from the lectin-agarose derivatives were ... | 1988 | 3191945 |
| pneumovirus-like characteristics of the mrna and proteins of turkey rhinotracheitis virus. | electronmicroscopy has indicated that turkey rhinotracheitis virus (trtv), the causative agent of an acute respiratory disease in turkeys, is a member of the paramyxoviridae family. to determine if trtv belongs to one of the three defined genera of this family (paramyxovirus, morbillivirus and pneumovirus) we have analysed the rna and proteins induced during replication of trtv in vero cells. following replication in the presence of actinomycin d 10 polyadenylated rna bands, ranging in mr from 0 ... | 1988 | 3195218 |
| an ultrastructural study of the morphology and lectin-binding properties of human mast cell granules. | the morphological characteristics and lectin-binding properties of mast cell granules from four human neurofibromata are described. ultrastructural examination of the granules revealed that some contained dense cores, others had membranous configurations and some forms were intermediate between the two. a round electron-lucent area was present in some granules. after treatment with biotinylated lectins (10 micrograms ml-1) followed by an avidin-peroxidase revealing system (5 micrograms ml-1 in 0 ... | 1988 | 3198420 |
| [localization of glycoconjugates in tissues of mammals revealed by means of labeled lectins]. | histochemical peculiarities on binding of castor-oil plant, soybean and lentil lectins with tissues of the mucous membrane in the stomach, small and large intestine have been studied in the human being, rat, mouse, as well as the lectins mentioned and the maize agglutinin with the nervous tissue of the rat cerebral tissue. the reactions are carried out with nonfixed cryostat and deparaffinized histological slices. lectins labelled with horseradish peroxidase are used. certain specific peculiarit ... | 1988 | 3207398 |
| affinity chromatography of fibrinogen on lens culinaris agglutinin immobilized on cnbr-activated sepharose: study of the active groups involved in nonspecific adsorption. | fibrinogen binds specifically to lens culinaris agglutinin coupled to cnbr-activated sepharose. however, a fraction of the retained fibrinogen remains tightly bound to the gel and is eluted only by electrophoretic desorption. the irreversible binding of fibrinogen results from the interaction of fibrinogen specifically bound to the immobilized lectin with some reactive groups still present on the sepharose matrix. therefore, the active groups present on lectin-sepharose after different treatment ... | 1988 | 3218727 |
| comparative chromatographic study of modifications of brush-border membrane vesicles induced by an essential fatty acid-deficient diet. | the surface properties of small intestine brush-border membranes (bbms) were examined by frontal affinity chromatography using three types of unsolubilized ligands: phlorizin polymer, immobilized lectins and linolenic acid bound to agarose gel. bbm vesicles were purified from piglets fed a corn oil diet (control diet) or a hydrogenated coconut oil diet. the second diet was representative of a deficient supply of essential polyunsaturated fatty acid (epufa). it induced a marked decrease in 18:2n- ... | 1988 | 3220917 |
| heterogeneity in copper and glycan content of ceruloplasmin in human serum differs in health and disease. | crossed immunoelectrophoresis of human serum revealed two heterogeneity types of ceruloplasmin with different electrophoretic migration. the two types both consisted of peptides with mr 150,000, 100,000 and 45,000, which were interpreted as native ceruloplasmin and two hydrolytic fragments. the two types were different in copper content, and one type could reversibly be changed into the other. the glycan microheterogeneity of ceruloplasmin was analyzed by crossed affinoimmunoelectrophoresis with ... | 1988 | 3234365 |
| [t-cell immunodeficiency in mice receiving lectin and cyclophosphamide]. | cyclophosphamide injections to mice following t cell mitogen (lectins from lens culinaris and concanavalin a) were shown to suppress (20-40-fold) thymus-dependent response to srbc. at the same time no damage-specific and polyclonal response to thymus-independent antigen and polyclonal activator of b cells--lipopolysaccharide--has been observed. injections of lectin and cyclophosphamide to mice prevented the onset of dth reaction to srbc and induction of antigen-specific dth suppressor cells. thu ... | 1988 | 3261177 |
| the rat t-cell differentiation marker rt6.1 is more polymorphic than its alloantigenic counterpart rt6.2. | utilizing allotype-specific antibodies to immunoprecipitate rt6.2 from da.6b rat lymphocyte lysates, we have shown this antigen recently to be composed of two related, non-glycosylated polypeptides with apparent molecular weights (mw) of 24,000 and 26,000 (reducing conditions), which evidently are anchored in the cell membrane by covalent linkage to phosphatidylinositol. the present report shows that rt6.1 allotype-specific antibodies precipitate a more complex pattern of bands from lew.6a rat l ... | 1988 | 3263942 |
| regulatory effects of human ige-binding factors in the ige synthesis by human and rat lymphocytes. | we have previously established human t cell hybridomas which produce ige-binding factors. incubation of one of the t cell hybridomas, 166a2, with human ige dimer in the presence of 1 microgram/ml bradykinin resulted in the formation of ige-binding factors having affinity for lentil lectin. the factors selectively enhanced both ige-forming cell responses of rat mesenteric lymph node (mln) cells and spontaneous ige synthesis by human peripheral blood b cells of atopic patients, without affecting t ... | 1988 | 3264529 |
| cell surface biochemical and metastatic properties of lens culinaris hemagglutinin-binding variants of a murine large cell lymphoma. | using a low metastatic potential parental (p) line of the murine large cell lymphoma raw117 and a highly metastatic in vivo-selected liver-colonizing subline (h10), we examined the relationship between cell surface glycoprotein expression and metastasis. the highly metastatic h10 cells showed loss of the major rna tumor virus envelope glycoprotein gp70 and increased expression of a concanavalin a and lens culinaris hemagglutinin (lch)-binding glycoprotein of mr approximately 15,000 (gp150) by le ... | 1988 | 3265705 |
| application of glycemic index to mixed meals. | plasma glucose and insulin responses to six different meals were determined and compared with values predicted by published glycemic indices of the component foods. the test meals were of different ethnic origins: indian (lentil curry with rice), italian (spaghetti bolognaise), chinese (stir-fried vegetables and chicken with rice), greek (lentil stew), western (sirloin chop and vegetables); and lebanese (sandwich with unleavened bread and hummos). eight healthy volunteers were given 50 g carbohy ... | 1988 | 3276136 |
| lectin-binding histochemistry of intracellular and extracellular glycoconjugates of the reserve cell zone of growth plate cartilage. | the distribution of intracellular and extracellular lectin-binding glycoconjugates of the reserve cell zone of growth plate cartilage was studied in the distal radial growth plate of 4-week-old yucatan swine using a postembedment method on epon-embedded sections. direct comparisons were made to articular, tracheal, and auricular cartilages not involved in endochondral ossification. all patterns of lectin binding that in the growth plate were restricted to the reserve cell zone were also patterns ... | 1988 | 3278077 |
| investigations on the binding of erythrogenic toxin a of streptococcus pyogenes on human peripheral blood lymphocytes. ii. identification of toxin-binding lymphocytes and characterization of the receptor. | gold complexes of erythrogenic toxin a (eta) of streptococcus pyogenes (strain ny-5) were bound to human lymphocytes that could be characterized as t cells by rosetting or labelling with okt 3 antibody and anti-mouse-igg-gold. using okt 4 and okt 8 antibodies, respectively, it was demonstrated that binding of eta was confined to the t helper/inducer cell subpopulation. treatment of lymphocytes with neuraminidase enhanced the binding of eta-gold, whereas trypsin treatment resulted in its reductio ... | 1988 | 3287805 |
| nephritogenicity and immunocytochemical localization of the 70-kilodalton glycoprotein subunit (gp70) of heymann antigen. | heymann nephritis (hn) is an experimentally induced glomerulonephropathy of the rat characterized by subepithelial immune deposits and proteinuria. immunization with a complex multimeric glycoprotein, gp600, comprising four subunits gp330, gp140, gp110, and gp70 has been shown to induce the complete form of the disease including proteinuria. examination of three different batches of heterologous anti-gp600 antisera by immunoblot technique showed that the reactivity toward gp70 was dominant and c ... | 1988 | 3289803 |
| heterogeneity of human natural killer cells with respect to lectin-binding ability. | the binding ability of peanut agglutinin (pna), lentil agglutinin (len), soybean agglutinin (sba), wheat germ agglutinin (wga), and asparagus pea agglutinin (asp) to human natural killer (nk) cells with the use of the double-marker immunofluorescence technique was studied. for identification of nk cells, vep 13 (dc 16) monoclonal antibody was used. the receptor for pna lectin was shown exclusively after neuraminidase treatment of cells, and vep 13 antigen was neuraminidase resistant. the majorit ... | 1987 | 3295353 |
| bulk purification of a naturally occurring soluble form of rt1-a class i major histocompatibility complex antigens from da rat liver, and studies of specific immunosuppression. | we describe the bulk purification of a water-soluble form of rt1-a class i mhc antigens from aqueous extracts of da liver. using a combination of monoclonal antibody affinity, lentil lectin affinity, and gel permeation chromatography, we were able to obtain large quantities of pure water-soluble rt1-a antigens. typically, from 40 da livers, 0.5 mg of pure antigen with antigen activity equivalent to 7 x 10(9) nucleated da spleen cells was obtained. the water-soluble rt1-a molecule had a discrete ... | 1987 | 3299912 |
| membrane glycophorins of dantu blood group erythrocytes. | glycophorins of erythrocytes of two unrelated individuals who exhibit the dantu blood group phenotype were studied. immunoblots indicated that erythrocytes of each individual contained a complement of a normal alpha-glycophorin (glycophorin a) and a variant n-glycophorin. delta-glycophorin (glycophorin b) was present in one donor's cells but not the other's; the s and n phenotypes of the latter's erythrocytes may derive from the variant glycophorin. the variant glycophorin is of a smaller size, ... | 1987 | 3305497 |
| boar sperm surface glycoproteins: isolation, localization, and temporal expression during spermatogenesis. | boar sperm glycoprotein fractions were isolated by lens culinaris hemagglutinin affinity chromatography of detergent-solubilized ejaculated spermatozoa, followed by preparative sodium dodecyl sulfate (sds) polyacrylamide gel electrophoresis. in order to develop methods for further investigations of the sperm proteins, we proceeded with two of the isolated glycoproteins. antibodies were raised in female rabbits against each of the two sperm glycoproteins. by a combination of immunosorbent chromat ... | 1987 | 3333754 |
| purification, composition, and structure of macrophage adhesion molecule. | macrophage adhesion molecule (mam) is a surface heterodimer consisting of the trypsin- and plasmin-sensitive glycopeptide gp160 (mam-alpha) and the glycopeptide gp93 (mam-beta). mam, which is the guinea pig analogue of mo1 and mac-1, was purified from detergent lysates of peritoneal neutrophils by lentil lectin chromatography and m2-antibody chromatography. the pure heterodimer molecule was dissociated by acidic conditions (ph 3.5), and mam-alpha and mam-beta were separated by m7-antibody chroma ... | 1988 | 3349044 |
| a quantitative method for gdp-l-fuc:n-acetyl-beta-d-glucosaminide alpha 1----6fucosyltransferase activity with lectin affinity chromatography. | a quantitative method for the activity of gdp-l-fuc:n-acetyl-beta-d-glucosaminide alpha 1----6fucosyltransferase has been developed using a well-characterized substrate to which other fucosyltransferases fail to transfer and lentil lectin-sepharose, which will bind this substrate only after fucosylation of the asparagine-linked n-acetylglucosamine. the enzyme was extracted from human skin fibroblasts and incubated with gdp-[14c]fucose and a specific substrate, asialo-agalactotransferrin glycopep ... | 1988 | 3364733 |
| binding site of the rat liver specific monoclonal antibody. | a rat liver-specific antigen (rlsa) lost its binding ability to the corresponding monoclonal antibody after treatment with n-glycanase or sialidase, which suggested that the specific binding site might be in a portion of the sugar chain containing sialic acid. the specific antigen reacted with wheat germ agglutinin, lentil lectin, erythroagglutinating phytohemagglutinin and ricinus communis agglutinin, but not with concanavalin a or peanut agglutinin. these results suggest that the specific anti ... | 1988 | 3377770 |
| isoelectric properties, lectin binding characteristics and biological activities of neuraminidase-treated rat lh components. | the present study was performed to evaluate the different carbohydrate structure of rat lh isoelectric components related to their intrinsic biological activities. terminal sialic acid residues were essential to the formation of multiple lh components observed in the isoelectric focussing profile, which was proved by their interaction with ricinus communis agglutinin-120 following neuraminidase treatment, and the conversion of component f (pi, 10.0) to less alkaline components after incubation w ... | 1988 | 3381629 |
| analysis of cell surface glycoprotein changes related to hematopoietic differentiation. | a high-resolution technique has been used to study differentiation-related and leukemia-associated glycoproteins. cells are labeled with the membrane-impermeable probe sulfo-n-hydroxysuccinimidyl-biotin. nonionic detergent extracts are subjected to affinity chromatography on a number of immobilized lectins and after polyacrylamide gel electrophoresis in sodium dodecyl sulfate (sds-page) and western transfer, the biotin-labeled glycoproteins are visualized by using avidin-horseradish peroxidase a ... | 1988 | 3392108 |
| the identification of membrane glycoconjugates in leishmania species. | the membrane glycoconjugates of 8 different species of leishmania were compared by lectin blotting. five different lectins with various sugar specificities were examined: concanavalin a, lens culinaris, ricinus communis, soybean agglutinin, and peanut agglutinin. concanavalin a and lens culinaris reacted with every leishmania tested. the patterns observed for these 2 lectins, as well as the various species of parasites, were different. however, a common 41,000-52,000 and a 160,000-185,000 mr com ... | 1988 | 3397816 |
| complex-type glycoproteins synthesized in the subcommissural organ of mammals. light- and electron-microscopic investigations by use of lectins. | the secretory activity in the subcommissural organ (sco) of the sheep and cow was examined by means of lectin histochemistry and cytochemistry. among the various lectins tested. concanavalin a (con a) revealed glycoproteins rich in mannosyl residues in the rough endoplasmic reticulum of ependymal and hypendymal cells. one of these con a-positive glycoproteins may represent the precursor of the specific secretory component elaborated in the sco, giving rise to reissner's fiber. lens culinaris agg ... | 1988 | 3409290 |
| lectin-induced adhesion of human platelets to glass. comparison with the lectin-induced aggregation. | effects of phytohaemagglutinin (pha), concanavalin a (con a) and lens culinaris (lens) lectins on the adhesion of human platelets to glass surface were studied. all the three lectins tested enhanced platelet adhesion to glass. concentration-effect curves showed that maximum effect was reached at about 25-30 micrograms/ml. in the presence of autologous plasma both spontaneous and lectin-induced adhesion to glass were diminished. the inhibitory effect of kcn, nan3, cytochalasin b and partly of col ... | 1988 | 3417184 |
| aflatoxins and fungal flora in lentil (lens esculenta l.). | fungal flora of 20 samples of lentil seeds collected from assiut governorate, egypt, were studied. seventeen genera and 13 species were isolated on glucose- (15 genera and 27 species), cellulose- (15 genera and 25 species)- czapek's agar media at 28 degrees c. the most common species were as follows: on glucose-czapek's agar, aspergillus fumigatus, a. niger, a. flavus, a. terreus, penicillium notatum and rhizopus oryzae and on cellulose agar, a. fumigatus, a. niger, a. flavus and p. notatum. thi ... | 1988 | 3419482 |
| comparisons of labeling efficiency, biological activity and biodistribution among 125i-, 67ga-dtpa-and 67ga-dfo-lectins. | the labeling efficiency, biological activity and biodistribution of 125i labeled and 67ga chelating agent conjugated lectins were investigated. pisum sativum agglutinin (psa) and lens culinaris agglutinin (lch) were efficiently labeled with 67ga using bifunctional chelating agents such as diethylenetriaminepentaacetic acid (dtpa) and deferoxamine (dfo), whereas labeling with 125i was significantly less efficient. the agglutinating activity of these lectins towards ehrlich ascites tumor (eat) cel ... | 1987 | 3428286 |
| efficacy of mung bean (lentil) and pop rice based rehydration solutions in comparison with the standard glucose electrolyte solution. | children with acute diarrhea and moderate dehydration between 3 months and 5 years of age were randomly assigned to receive treatment with standard who oral rehydration solution (ors) (n = 33) and two other solutions in which the 20 g/l glucose was substituted by 50 g/l of pop rice (n = 31) and 60 g/l of mung bean (lentil) powder (n = 29). satisfactory oral rehydration, as assessed clinically and by changes in pcv and total serum solids (tss), was achieved in 90.9% with who ors, 96.8% with pop r ... | 1987 | 3430248 |
| enhanced glycosylation of a melanoma cell surface glycoprotein by retinoic acid: carbohydrate chain analysis by lectin binding. | retinoic acid (ra) inhibits the growth of mouse s91-c2 melanoma cells and enhances the glycosylation of a cell surface sialoglycoprotein (gp160). the present study analyzed the binding of 125i-labeled lectins to gp160 within polyacrylamide slab gels after electrophoretic separation of cellular macromolecules. wheat germ agglutinin (wga) and concanavalin a (con a) bound to gp160 of ra-treated cells (ra-gp160) more extensively than to gp160 of control cells (c-gp160). lens culinaris hemagglutinin ... | 1987 | 3435894 |
| the chromatographic heterogeneity of rat transferrin on immobilized concanavalin a and lentil lectin. | a procedure was developed for the isolation of the microheterogeneous forms of rat transferrin consisting of anion-exchange and serial lectin affinity chromatographies. by deploying this technique, four to five different anionic species of the protein were detected in plasma. the two major components obtained, which encompassed 92-94% of the plasma transferrin, were further studied by sequential lectin chromatography. the larger of the two, representing 60-63% of plasma transferrin, was bound by ... | 1987 | 3442627 |
| determination of attachment sites for n-linked carbohydrate groups of class ii histocompatibility alpha-chain and analysis of possible o-linked glycosylation of alpha- and gamma-chains. | two glycopeptide fractions were isolated from a tryptic digest of the human class ii antigen alpha-subunit, by chromatography on lens culinaris and ricinus communis lectin columns, respectively. partial nh2-terminal sequence analysis of radiochemically labelled glycopeptide fractions allowed alignment with two stretches of the deduced dr alpha sequence, each encompassing a signal for n-linked glycosylation, i.e. asn-x-thr(ser). the fraction displaying affinity for l. culinaris lectin was suscept ... | 1986 | 3457263 |
| characterization of the fertilization antigen 1 for the development of a contraceptive vaccine. | a fertilization antigen, fa-1, was purified from either deoxycholate- or lithium diiodosalicylate-solubilized murine testes by immunoaffinity chromatography using a monoclonal antibody, ma-24, which inhibited fertilization in vitro. the fa-1 was recovered at high (11.4) or low (2.8) ph using stepwise elution procedures of the deoxycholate or lithium diiodosalicylate extracts, respectively. both of these fractions showed a single band of 47 kda when analyzed by nadodso4/page and silver staining. ... | 1986 | 3461457 |
| comparison of the n-linked glycopeptides of dqw1 and dr1 molecules. | hla class ii molecules have been isolated from a [3h]mannose-labeled gm3104 b lymphoblastoid cell line with the phenotype dqw1, dr1. the dqw1 molecules were purified by affinity to 77-34 igg specifically reactive with the dqw1 specificity. the dr1 molecules were separated into two subsets, dr1a (70 to 80%) and dr1b (20 to 30%), by sequential affinity to 21r5-igg and 21w4-igg sepharose. the alpha- and beta-chains of [3h]mannose-labeled dqw1, dr1a, and dr1b molecules were separated by sds-page and ... | 1986 | 3486905 |
| structural analysis of the oligosaccharides of dr1 and dqw1 molecules. | the major glycopeptide fractions of the alpha- and beta-chains of hla-dr1 and dqw1 molecules were isolated on columns of immobilized concanavalin a (con a), lens culinaris (lens), ricinus communis agglutinin type i (rca), and leuko-phytohemagglutinin. oligosaccharides were prepared from these fractions by enzymatic digestion with endoglycosidases h or f and were analyzed on bio-gel p-6. the glycopeptides tightly bound to con a (cona iii) were mostly associated with alpha-chains and were resolved ... | 1986 | 3486906 |
| characterization of lectin-binding lymphocytes in goats with caseous lymphadenitis. | numbers of lymphoid cells bearing markers for peanut agglutinin, wheat germ agglutinin, and anti-immunoglobulin g in healthy goats and in goats with caseous lymphadenitis were determined by use of immunofluorescent techniques. results indicated that, when compared with healthy goats, diseased goats had normal numbers of anti-immunoglobulin g-positive cells (b cells), had significantly reduced numbers of peanut agglutinin-positive cells (t cells), and had fewer cells with receptor sites for lens ... | 1986 | 3487998 |
| purification of human macrophage colony stimulating factor (csf-1) from medium conditioned by pancreatic carcinoma cells. | colony stimulating factor-1 has been purified to apparent homogeneity from the serum-free medium conditioned by cultured human pancreatic carcinoma cells which had been induced with phorbol myristate acetate. the purification scheme consisted of sequential steps of batchwise adsorption to calcium phosphate gel, adsorption to lentil lectin-sepharose, binding to immobilized antibodies, hydrophobic interaction chromatography, and reversed-phase high-performance liquid chromatography. the purified g ... | 1986 | 3488735 |
| characterization of human t cell-derived ige-potentiating factor. | we have previously shown that fc epsilon receptor-positive (fc epsilon r+) t cell lines from patients with the hyper ige syndrome secrete ige-binding factors which selectively enhance ige but not igg synthesis in cultures of b cells obtained from patients with allergic rhinitis but not from nonatopic subject. in the present study we have tested the effect of supernatants from fc epsilon r+ t cell lines on a large panel of b cells from atopic patients (n = 20). we found that ige synthesis was sel ... | 1986 | 3488913 |
| binding of a synthetic analogue of mitogenic bacterial lipoprotein to murine major histocompatibility complex (mhc) gene products. | lipoprotein from the outer membrane of escherichia coli and a synthetic analogue of its n-terminal lipopeptide part, tripalmitoyl-pentapeptide, constitute potent mitogens and polyclonal activators of murine b-lymphocytes in vitro. when entering the circulation after intravenous administration in experimental animals, they interact with the humoral and cellular elements of the blood, which results in splenomegaly and b-lymphocyte activation in vivo. we investigated lipopeptide-binding proteins in ... | 1986 | 3491614 |
| analysis of asparagine-linked oligosaccharide structures of chronic lymphocytic leukemia cells. | the asparagine-linked oligosaccharide structures of chronic lymphocytic leukemia cells (cll) were studied by sequential lectin affinity chromatography. glycopeptides were isolated from a concanavalin a (cona)-binding glycoprotein fraction prepared from a soluble cll extract. this fraction, which contained 1.8% of the proteins present in the soluble extract, greater than 30 polypeptides revealed by sds-page analysis, and known cell surface antigens such as hla-dr and p85 glycoprotein, must includ ... | 1987 | 3498886 |
| the carbohydrate moieties of suppressor igg-binding factor released by murine t cells. | the carbohydrate moieties of murine igg-binding factor (igg-bf) were studied using lectins binding n-glycosylated sequences such as concanavalin a (con a), lens culinaris agglutinin (lca), and wheat germ agglutinin (wga), and lectins binding o-glycosylated sequences such as peanut agglutinin (pna) and helix pomatia agglutinin (hpa). sources of igg-bf were: (1) supernatants from t2d4, a t cell hybridoma constitutively producing igg-bf, and (2) factor purified by affinity chromatography on rabbit ... | 1987 | 3500399 |
| thrombospondin in essential thrombocythemia. | essential thrombocythemia is a myeloproliferative disorder characterized by frequent bleeding and thrombotic complications. on a molecular level, two abnormalities of platelet thrombospondin have been identified: abnormal glycosylation of the intact 185,000-dalton chain has been detected and a shortened form of the thrombospondin chain is present. we have used two monoclonal antibodies and lens culinaris lectin to probe the structure of thrombospondin in the platelets from three patients with es ... | 1986 | 3510684 |
| fluorochrome-coupled lectins reveal distinct cellular domains in human epidermis. | the distribution of saccharide moieties in human interfollicular epidermis was studied with fluorochrome-coupled lectins. in frozen sections concanavalin a (con a), lens culinaris agglutinin (lca), ricinus communis agglutinin i (rcai), and wheat germ agglutinin (wga) stained intensively both dermis and viable epidermal cell layers, whereas peanut agglutinin (pna) bound only to living epidermal cell layers. ulex europaeus agglutinin i (ueai) bound to dermal endothelial cells and upper cell layers ... | 1986 | 3512692 |
| membrane glycophorins in sta blood group erythrocytes. | structural and immunochemical studies of glycophorins isolated from erythrocytes of an individual homozygous for the m sta blood group phenotype are described. reactivities with specific monoclonal antibodies indicated that two major m and n glycophorins were present. the m and n sta glycophorins were resolved by lens culinaris lectin affinity chromatography. the n species was not held on the lectin but the m species, like control alpha glycophorins, was retained and could be eluted with alpha-m ... | 1986 | 3514617 |
| murine iga binding factors (iga-bf) suppressing iga production: characterization and target specificity of iga-bf. | chemical and functional properties of iga binding factor(s) (iga-bf) from both murine con a-activated spleen cells and fc gamma r+, fc alpha r+ t hybridoma cells (t2d4) were studied. iga-bf produced from the cells after preculture with iga were purified with iga-sepharose. purified iga-bf inhibited the binding of iga to fc alpha r+ l5178y t lymphoma cells, and class-specifically suppressed in vitro iga synthesis of the pokeweed mitogen (pwm)-stimulated murine spleen cells. both iga-specific supp ... | 1986 | 3514749 |
| purification and characterization of a neutrophil chemotactic factor from dirofilaria immitis. | a neutrophil chemotactic factor (ncf-di) was purified from a crude extract of dirofilaria immitis adult worm by a combination of anion-exchange chromatography on de52 and gel filtration on sephacryl s-200. ncf-di showed a single protein band by both polyacrylamide gel electrophoresis (page) and sodium dodecyl sulfate (sds) page. the molecular weight of ncf-di was estimated to be 17,000 by gel filtration on sephadex g-150, and 14,000 by sds-page. ncf-di was an acidic protein with isoelectric poin ... | 1986 | 3525795 |
| [purification and properties of bordetella pertussis toxin]. | the modified method for the isolation and purification of b. pertussis toxin has been proposed. chromatography with the use of hydroxylapatite and lentil lectin--sepharose 4b has permitted the isolation of the preparation purified 600 times. its molecular weight is about 90,000. the preparation has been found to possess leukocytosis-stimulating, histamine-sensitising and hemagglutinating activity. electrophoretic analysis has revealed that the isolated substance consists of four subunits with mo ... | 1986 | 3529763 |
| antigenic pattern of human brain glycoproteins as described by monoclonal antibodies. | a battery of monoclonal antibodies was raised against a preparation of lentil lectin-binding membrane glycoproteins from human brain. out of 26 established hybridomas, nine produced antibodies against the human thy-1 antigen. for the remaining 17 lines, reactivity with at least six other antigens could be identified after immunoprecipitation and immunoblotting. several of the antigens were di- or trimeric, mainly in the molecular weight range of 60-120 kda. two of the antibodies were reactive wi ... | 1987 | 3543222 |
| production and glycosylation of sperm constitutive proteins in the lizard lacerta vivipara. evolution during the reproductive period. | from epididymal fluid samples taken at three different times during the reproductive period (early april, late april, mid-may), the soluble proteins were separated with one dimensional electrophoresis on polyacrylamide gel. their evolution was studied: firstly quantitatively, after staining with coomassie blue, or, for one protein (the "l" protein), by immunodetection; secondly, according to their glycosylation after transfer to nitrocellulose and treatment with a set of labelled lectins: from w ... | 1987 | 3568614 |
| differential binding of mannose-specific lectins to the carbohydrate chains of fibrinogen domains d and e. | the interaction of fibrinogen with the mannose-specific lectins concanavalin a (cona), its acetyl derivative (ac-cona) and lens culinaris agglutinin (lch) was studied. both cona and lch interact specifically with individual fibrinogen b beta and gamma chains and with denatured fragments d and e. however, analysis of the binding data shows that four moles of ac-cona are bound per mole of fibrinogen with two sets of binding sites (kd1 = 2.4 microm and kd2 = 16.6 microm; n1 = n2 = 2) while only two ... | 2013 | 3569289 |
| characterization of a rat salivary sialoglycoprotein complex which agglutinates streptococcus mutans. | rat saliva agglutinated streptococcus mutans ingbritt and nctc 10449 and streptococcus sanguis nctc 7864 but not s. mutans nctc 10921, gs 5, or lm 7, streptococcus sobrinus 6715-13 or omz 65, or streptococcus cricetus hs 6, as measured turbidometrically. the specificity of agglutination by rat saliva was the same as that by human saliva. agglutination was associated with a mucin complex (rat salivary agglutinin complex [rs-a]) of sulfated sialoglycoproteins, with a trace of associated lipid and ... | 1987 | 3570462 |
| evaluation of structural and secretory glycoconjugates in normal human jejunum by means of lectin histochemistry. | the labelling pattern of eight lectins was studied in jejunal samples from ten normal subjects, in order to define the normal distribution of structural and secretory glycoconjugates in the small bowel. the following lectins were studied by means of a peroxidase technique on formalin-fixed samples: arachis hypogaea, ricinus communis, canavalia ensiformis, lens culinaris, phaseolus vulgaris, triticum vulgaris, ulex europaeus, dolichos biflorus. phaseolus vulgaris reacted with goblet cell mucus th ... | 1987 | 3570880 |
| characterization of tumor rejection antigen molecules of chemically induced murine colon tumor c-c26. | the molecular nature of a tumor-specific transplantation antigen (tsta) of a chemically induced balb/c mouse colon tumor c-c26 was investigated. the antigen was noncytolytically extracted by 2.5% n-butanol treatment of the cells. crude butanol extract from c-c26, but not from colon tumor c-c51 and fibrosarcoma meth-a of balb/c mice could provide protection against the challenged c-c26 tumor in the transplantation experiment. crude butanol extract from another syngeneic colon tumor c-c36 also ind ... | 1987 | 3581064 |
| comparative biochemical analysis of purified s-antigen from human, bovine, porcine and rat retina. | highly purified s-antigen from human, bovine, porcine and rat retina was used to ascertain the molecular weights, isoelectric points, amino-acid composition and presence of carbohydrate moieties. all s-antigen preparations comigrate to 50,000 mw on sds-polyacrylamide gels and demonstrate microheterogeneity upon isoelectric focusing. human s-antigen exhibits two bands at ph 5.9 and 5.6, whilst with bovine s-antigen a broad band at ph 5.9 was observed. porcine and rat s-antigen gave four bands, fo ... | 1987 | 3582507 |
| leishmania braziliensis: protein, carbohydrate, and antigen differences between log phase and stationary phase promastigotes in vitro. | when leishmania species are grown in vitro, parasites from the stationary phase differ from those in log phase growth in being more infective and more resistant to complement and macrophage mediated killing. in the present study, log phase and stationary phase promastigotes of leishmania braziliensis panamensis were compared at the molecular level. differences in polypeptide and glycoprotein composition and antigenicity between log and stationary phase promastigotes of l. b. panamensis were dete ... | 1987 | 3582573 |
| reconstitution of the glucose transporter from rat skeletal muscle. | as a step in the purification and characterization of the glucose transporter from rat skeletal muscle, we have reconstituted glucose transport activity in liposomes. plasma membranes were prepared from skeletal muscle which display d-glucose reversible binding of cytochalasin b (10 pmol sites/mg protein; kd = 0.3 microm). older rats gave a slightly lower specific activity and much lower yield of sites per g muscle than young rats. glucose transport activity was reconstituted into liposomes by t ... | 1987 | 3586860 |
| gerbich blood group deficiency of the ge:-1,-2,-3 and ge:-1,-2,3 types. immunochemical study and genomic analysis with cdna probes. | human erythrocytes carry several transmembrane glycoproteins, among which the two minor species associated with the blood group gerbich (ge) antigens, gp c and gp d, play pivotal role since they interact with the membrane cytoskeleton and contribute to maintain the normal red cell shape. on the red cells from two categories of homozygous donors lacking the ge determinants (ge:-1,-2,-3 and ge:-1,-2,3), gp c and gp d are missing but instead there is a new glycoprotein, easily detected by sds/polya ... | 1987 | 3595602 |
| microheterogeneity of lentil seedling (lens culinaris) amine oxidase. | purified lentil seedling amino oxidase (lsao) is homogeneous in the analytical ultracentrifuge, but shows heterogeneity in gel-filtration hplc and in page. two components were obtained from hplc and page, but at least 6-7 subforms were seen by electrofocusing techniques. the chromatographic and electrophoretic forms are not interconvertible, indicating the presence of covalent differences. the electrophoretic pattern, but not the chromatographic pattern, is modified by treating the enzyme with a ... | 2012 | 3597049 |
| litomosoides carinii: characterization of surface carbohydrates of microfilariae and infective larvae. | the carbohydrate moieties of microfilariae (mf) and infective larvae (l3) have been investigated by lectin-binding technique. mf derived from three sources, namely, uteri (in utero), released in vitro from adults and from blood of rodents infected with litomosoides carinii were examined by using fluoresceinated lectins. wheat germ agglutinin (wga) bound to these mf and the binding was inhibited by n-acetyl glucosamine. in addition to wga, concanavalin a (con a) and lentil lectin (lch) bound to i ... | 1987 | 3602835 |
| the dinitrophenyl group as a selective label in high-performance liquid chromatography of peptides. | 1-fluoro-2,4-dinitrobenzene can be used to selectively label histidine, tyrosine, and cysteine residues in maleylated proteins. the usefulness of the resulting chromophores for peptide mapping by high-performance liquid chromatography was demonstrated with the lectin from sainfoin (onobrychis viciifolia). the 2,4-dinitrophenyl (dnp) label also can be used in a hydrophobic modulation approach as the mobility of a labeled model peptide changes considerably when its dnp group is removed by thiolysi ... | 1987 | 3605591 |
| glycophorins of human erythroleukemic k562 cells. | glycophorins related to alpha glycophorin, of the human erythrocyte membrane, were isolated from human erythroleukemic k562 cells. the glycophorins were purified using sodium dodecyl sulfate (sds)/trichloroacetic acid fractionation and folch and hot phenol extractions. 0.1-0.2 micrograms was obtained/10(8) cells, or approximately a 15% yield. sds-gel electrophoresis revealed a pattern similar to erythrocyte alpha glycophorin except for the slower mobility of the glycophorin monomer. two populati ... | 1987 | 3606125 |
| lectin histochemistry of mammalian endothelium. | lectin-histochemical studies were performed on formalin-fixed, paraffin-embedded tissues from ten mammalian species to demonstrate the pattern of carbohydrate residues in vascular endothelium. ten different biotinylated lectins were used as probes and avidin-biotin-peroxidase complex (abc) was used as visualant. ricinus communis agglutinin-i (rca-i) and wheat germ agglutinin (wga) stained vascular endothelium in all species. peanut agglutinin (pna) stained vascular endothelium in all species onl ... | 1987 | 3610672 |
| a lectin cytochemical study of glycoconjugates in the human retina. | the binding to morphologically normal human retina of eleven biotin- or peroxidase-coupled lectins with different carbohydrate specificities was studied. eight formalin-fixed and paraffin-embedded eyes were examined. photoreceptor cells bound lens culinaris (lca), wheat germ (wga), peanut (pna) and ricinus communis (rcai) agglutinins, and concanavalin a (cona). the outer segment region was labeled more strongly that the inner segment region, and pna labeled only cones. all these lectins except p ... | 1987 | 3621302 |
| evaluation of the hydrogen breath test in man: definition and elimination of the early hydrogen peak. | after ingestion of a non-absorbable carbohydrate breath hydrogen excretion increases early at about 10 minutes, and again later when the ingested carbohydrate enters the caecum. the late rise has been used as a marker of mouth to caecum transit time, but the source of the early rise has not been satisfactorily explained. we studied in 60 healthy volunteers the source and frequency of the early rise in breath hydrogen after ingestion of a non-absorbable carbohydrate. after ingestion of either lac ... | 1987 | 3623219 |
| separation of carbohydrates and lectins on carbohydrate-immobilized resins. | a few carbohydrate-immobilized resins were prepared by coupling glycamines of mono- and disaccharides to an epoxy-activated methacrylate base, and the elution profiles of various carbohydrates and lectins from columns packed with the resultant resins were compared. the introduction of glucamine, maltamine or lactamine to the methacrylate base had little effect on the separation of stereoisomers of aldoses, though it enabled group separation of pentoses as well as 6-deoxyhexoses from hexoses. on ... | 1987 | 3624387 |
| energy utilization of supplemented cereal diets in human volunteers. | energy utilization was studied in human volunteers using different diets containing wheat flour supplemented by groundnut (arachis hypogaea), "masur" (lens culinaris), mung (phaseolus aureus) and gram (cicer arietinum) flour. digestible and metabolizable energies were determined for all the experimental diets. an improved energy digestibility was observed when wheat flour was supplemented with groundnut flour, and groundnut flour plus gram flour, i.e. 93.35 and 89.48%, respectively. percent dige ... | 1986 | 3632213 |
| detection of anomalies in cell-surface carbohydrates on thrombasthenic platelets using 125i-labeled lectins. | the composition of carbohydrates on the surface of platelets from a patient with glanzmann's thrombasthenia and from seven normal donors were determined and compared. to this end, binding studies were performed using nine different purified 125i-labeled lectins; concanavalin a, p-phytohaemagglutinin, wheat germ agglutinin, dolichos biflorus, pisum sativum, ricinus communis ii agglutinin, tetragonolobus purpureus, lens culinaris and soybean agglutinin. these studies show that thrombasthenic plate ... | 1986 | 3633202 |
| glycoproteins in the whorls of membrane produced by oligodendroglia in culture. | two glycoproteins of 99 kda and 77 kda which exhibit intense binding to wheat germ agglutinin have been purified from the whorls of membrane produced by oligodendroglia in culture. the whorls of membrane were isolated by gradient centrifugation from purified bovine oligodendroglia maintained in culture. the two glycoproteins were solubilized from the membranes using a non-ionic detergent and purified by sephadex lh-60 chromatography, wheat germ agglutinin affinity chromatography, and sds-polyacr ... | 1987 | 3651447 |
| metabolic stability of the fucose in rat transferrin. | the metabolic behaviour of the chitobiose core fucose that is a natural constituent of a large proportion of rat transferrin molecules was studied in rats comparatively to that of the polypeptide portion of the glycoprotein by using appropriate labels ([3h]fucose and 125i) and affinity chromatographic techniques (lentil-sepharose). no evidence was obtained to suggest that this residue is cleaved from the glycan in significant amounts before removal of the entire glycoprotein for catabolism. simi ... | 1987 | 3653406 |
| isolation from human chronic-lymphocytic-leukaemia cells of membrane glycoproteins associated with fc-receptor functions. physical parameters and production of polyclonal antibodies. | two membrane glycoproteins that bound immune complexes and inhibited fc-receptor- (fcr-)mediated functions in vitro were purified from human fcr+ chronic-lymphocytic-leukaemia cells. a multi-step purification was developed, consisting essentially in: (i) tween 40 extraction of crude cell membranes; (ii) solubilization of membrane fragments by renex-30; (iii) isolation of glycoproteins by affinity chromatography on lens culinaris haemagglutinin-sepharose; (iv) papain treatment of the eluted glyco ... | 1987 | 3663159 |
| evidence for glycoprotein components of the hepatocellular bile acid transporter. | the hepatocellular transporter, responsible for the uptake of bile acids and some foreign substances, can be shown to contain carbohydrate moieties. the hepatocellular uptake of cholate and phallotoxin is immediately inhibited by addition of wheat-germ agglutinin. concanavalin a and lentil lectin reduce the uptake in a time-dependent manner. apparently sialic acids or n-acetylglucosamine residues are involved in the translocation process. polypeptides (mr 50,000, 54,000) of the above transport s ... | 1987 | 3663657 |
| in vivo binding partners of the lens culinaris lectin. | the immobilized lectin from the lentil (lens culinaris) specifically binds two fractions out of the l. culinaris seed globulins. both fractions are displaced from the lectin at low ph values. in addition, fraction i fails to interact at high ionic strengths, and fraction ii in the presence of glucose or other lectin-specific sugars. the behaviour in zonal isoelectric precipitation and electrophoretical patterns indicate that both fractions represent subpopulations of the storage proteins. the in ... | 1987 | 3675873 |
| interaction of rat liver glucocorticoid receptor with lectins: is the glucocorticoid receptor a glycoprotein? | although glucocorticoid receptors have been extensively studied in a variety of tissues, the precise nature of the receptor protein still remains unknown. to further characterize this protein we assessed the effects of various lectins on [3h]dexamethasone binding to prepurified preparations of rat liver glucocorticoid receptor. among the lectins tested only ulex europeus and lens culinaris induced a concentration-related decrease in [3h]dexamethasone binding. following ulex europeus or lens culi ... | 1986 | 3702409 |
| similarities in glycosylation of human neuroblastoma tumors and cell lines. | the presence of fucosyl residues linked alpha 1----3(4) to n-acetylglucosamine was demonstrated on the oligosaccharides from glycoproteins of 11 human neuroblastoma tumors from ten different patients. this finding is in complete agreement with the previous report that human neuroblastoma cell lines contained an unusually large proportion of metabolically incorporated l-[3h]fucose in this specific linkage (u. v. santer and m. c. glick, cancer res., 43:4159-4166, 1983). furthermore, the glycopepti ... | 1986 | 3708596 |
| purification and chemical composition of gpl115, the human lymphocyte surface sialoglycoprotein that is defective in wiskott-aldrich syndrome. | gpl115, the surface sialoglycoprotein that is defective in lymphocytes of wiskott-aldrich syndrome patients has been purified from large scale cultures of the lymphoblastoid line cem. the purification entails cell lysis and solubilization of gpl115 with the detergent nonidet p-40, sequential affinity chromatography on lentil lectin-sepharose, wheat germ lectin-sepharose, and, after treatment with sialidase, on peanut lectin-sepharose. sepharose cl-6b gel filtration removes residual protein conta ... | 1986 | 3711098 |
| lectin binding and the carbohydrate moieties present on newcastle disease virus strains. | lectin-binding profiles were developed for 14 strains of newcastle disease virus in order to determine the carbohydrate moieties associated with hemagglutination and to establish whether there are any associations between the carbohydrates present on the virus envelope and virulence. all strains of newcastle disease virus were bound by concanavalin a. other lectins bound the viruses differentially, but there was no pattern of binding that could be associated with viral virulence. the binding of ... | 1986 | 3729878 |
| suppression of low-dose streptozotocin-induced diabetes by immunomodulatory lectins. | the effect of immunomodulatory lectins on diabetes development following low-dose streptozotocin treatment in inbred mice was studied. all lectins administered had been shown previously to suppress immune reactivity. among plant lectins concanavalin a but not lens culinaris or wheat germ agglutinin partially suppressed hyperglycaemia following low-dose streptozotocin. a similar inhibitory effect was found for the staphylococcal enterotoxin b. finally, administration of an immunomodulatory lectin ... | 1986 | 3742941 |
| comparisons between true digestibility of total nitrogen and limiting amino acids in vegetable proteins fed to rats. | values (%) for true digestibility (td) of protein and individual amino acids in some vegetable proteins were determined by the rat balance (fecal) method. diets containing 8% crude protein (n x 6.25) from soaked and autoclaved samples of trapper and century field peas, lentil, pinto bean, seafarer bean, black bean or fababean and autoclaved samples of soybean, peanut, sunflower, rolled oat, rice + soybean and corn + pea were tested in two rat balance studies. in the case of blends, each protein ... | 1986 | 3746456 |
| biochemical investigation of the pathogenesis of heymann nephritis. | this study describes biochemical comparison of proximal tubule antigens from the brush border membrane (bbm) of dog and rat kidney. the purpose was to determine if a difference in bbm composition could explain the inability to produce either active or passive heymann nephritis in the dog. although the membrane composition as revealed by coomassie blue staining on 4 to 11 per cent polyacrylamide electrophoresis varied considerably between rats and dogs, polyclonal antibodies (rabbit anti-rat, rab ... | 1986 | 3747348 |
| characterization of surface glycoproteins on schistosoma mansoni adult worms by lectin affinity chromatography. | adult schistosoma mansoni were radiolabeled by direct radioiodination using the bolton-hunter reagent or by metabolic labeling using radioactive hexose precursors. tegumental material was extracted by freeze-thaw or by incubation in the non-ionic detergent nonidet p-40, then applied to chromatography columns containing the following immobilized lectins: con a, lentil lectin, wheat germ agglutinin, soybean agglutinin and the agglutinins from ricinus communis and helix pomatia. sds-page analysis o ... | 1986 | 3755470 |
| alteration by concanavalin a of the slow dissociable component in the human growth hormone-receptor interaction. | in mice liver plasma membranes (pm), the binding affinity of receptors for [125i] human growth hormone (hgh) was dependent on the association time: after 18 hours, a high affinity receptor form with ka = 6.8 x 10(9) m-1 accumulated and, as compared to after 1 hour, an increase up to 88%, in a slow dissociating component was observed. preincubation of pm with concanavalin a (con a) or other lectins from lens culinaris (lca), ricinus communis (rca i), wheat germ agglutinin (wga) specifically inhib ... | 1986 | 3758923 |
| affinity chromatography and sds-page studies of radiolabeled ige-binding and igg-binding factors generated from human lymphoblastoid cell lines. | soluble ige-binding and igg-binding factors were generated by 18-hour incubation at 4 degrees c of the human b cell lines rpmi 8866 and daudi. these cells express fc receptors for ige (fc epsilon r) and igg (fc gamma r), respectively. binding factors specifically inhibited fcr on both lymphocytes and monocytes, and bound to ig-sepharose supports. rpmi 8866 cells and daudi cells were radiolabeled with 125i by the lactoperoxidase method, and the soluble factors were labeled by the chloramine t met ... | 1986 | 3759254 |
| purification and characterization of the extracellular acid phosphatase of leishmania donovani. | a tartrate sensitive acid phosphatase activity was purified from culture supernatants of leishmania donovani promastigotes grown in a macromolecule-free defined medium. purification was accomplished by ultrafiltration, lentil lectin affinity chromatography, and sucrose density gradient ultracentrifugation. this enzyme was determined to be an acid glycoprotein containing 0.37 mg hexose per mg protein. a molecular weight of 134 000 by sucrose density gradient centrifugation was observed, although ... | 1986 | 3762593 |
| resonance energy transfer microscopy: observations of membrane-bound fluorescent probes in model membranes and in living cells. | a conventional fluorescence microscope was modified to observe the sites of resonance energy transfer (ret) between fluorescent probes in model membranes and in living cells. these modifications, and the parameters necessary to observe ret between membrane-bound fluorochromes, are detailed for a system that uses n-4-nitrobenzo-2-oxa-1,3-diazole (nbd) or fluorescein as the energy donor and sulforhodamine as the energy acceptor. the necessary parameters for ret in this system were first optimized ... | 1986 | 3771633 |
| concanavalin a and lens culinaris agglutinin binding patterns in normal and neoplastic sebaceous epithelium. | formalin-fixed, paraffin-embedded tissue sections of normal skin, sebaceous hyperplasia, nevus sebaceus, sebaceous adenoma, and sebaceous carcinoma were studied by means of biotinylated and fitc conjugated concanavalin a (con a) and lens culinaris agglutinin (lca). at relatively high concentrations of these lectins, all cutaneous epithelial cells were stained. as the concentration of lca was lowered, there was a corresponding decrease in the intensity of staining of all epithelial cells. with lo ... | 1986 | 3771872 |
| oligodendrocyte cell adhesion molecules are related to neural cell adhesion molecule (n-cam). | the glycoproteins responsible for calcium-dependent oligodendrocyte aggregation were purified and characterized. using detergent extraction, lentil-lectin-sepharose 4b affinity chromatography, and preparative gel electrophoresis, 3 proteins were purified to apparent homogeneity, with relative mrs of 120,000, 140,000, and 180,000. the aggregation assay showed that all 3 proteins had the ability to block antibody-mediated inhibition of oligodendrocyte aggregation. the 120,000 protein was the most ... | 1986 | 3772436 |
| [lectin binding of psoriatic skin]. | the aim of our study was the characterization of epidermal lectin binding pattern in psoriatic vs. non-psoriatic skin in order to reveal possible alterations of the glycocalyx composition of psoriatic keratinocytes. we used fluoroisothiocyanate-labeled lectins of canavalia ensiformis (cona), phaseolus vulgaris (pha), lens culinaris (lca), and helix pomatia (hpa). the binding pattern of psoriatic (involved and non-lesional) skin did not differ from the control samples in cona, pha, and lca. in ps ... | 1986 | 3776279 |
| analysis of normal human tonsil class ii antigen glycosylation by lectin affinity chromatography. | human class ii molecules include the hla-dr, -dq, and -dp alloantigens. each class ii molecule consists of two glycosylated polypeptide chains, the alpha chain and the beta chain. we have used lectin affinity analysis to investigate the glycosylation pattern of [3h]mannose-labeled glycopeptides derived from isolated alpha and beta chains of hla-dr and -dq molecules of normal tonsil cells. glycopeptides obtained by pronase digestion of each isolated chain were passed sequentially over columns of ... | 1986 | 3782152 |
| characterization of the carbohydrate moiety of clerodendron trichotomum lectins. its structure and reactivity toward plant lectins. | lectins were isolated from fruits and leaves of clerodendron trichotomum by affinity chromatography on lactamyl-sepharose. the purified lectins (c. trichotomum agglutinin: cta) were homogeneous on sds/polyacrylamide gel electrophoresis, and the carbohydrate moiety was characterized by physicochemical and immunochemical methods. the asparagine-linked oligosaccharides were released by treatment with n-oligosaccharide glycopeptidase (almond, ec 3.5.1.52) of peptic glycopeptides obtained from fruit ... | 1986 | 3792315 |
| changes in the glycosylation pattern during embryonic development of mouse kidney as revealed with lectin conjugates. | distribution of lectin-binding sites in adult and developing mouse kidney was studied with fluorochrome- and peroxidase-coupled lectins. effects of fixation methods on lectin-binding patterns were also compared. un-induced mesenchymal cells and ureter bud of the early metanephros reacted with concanavalin a, lens culinaris, ricinus communis i, and wheat germ agglutinins, whereas binding sites for both soybean and peanut (pna) agglutinins were seen only in ureter bud tissue. on induction, pna pos ... | 1987 | 3794309 |
| effects of horse and fetal calf serum on the expression of tumor-associated antigen and tumorigenicity of l5178y leukemia/lymphoma cells. | a tumor antigen (ta) associated with murine leukemia-lymphoma l5178y cells has been identified by the enzyme-linked immunosorbent assay (elisa) and indirect immunofluorescence (iif) techniques. the antigen was present in both non-solubilized and 0.5% np-40 solubilized membrane extracts. rabbit anti-l5178y lymphoma serum (rals), extensively absorbed with normal mouse tissues, identified ta in extracts of l5178y lymphoma and l5178y leukemia cells grown in horse serum (l5178y/hs), but not in extrac ... | 1986 | 3796035 |
| a structural analysis of the carbohydrate side chains on class i and class ii histocompatibility antigens of the swine facilitated by heteroantisera specific for the denatured polypeptides. | rabbit heteroantisera specific for the denatured glycoprotein subunits of swine class i and class ii major histocompatibility complex (mhc) antigens have been prepared and utilized to monitor changes in the mobilities of these polypeptides on sds-polyacrylamide gel electrophoresis subsequent to various deglycosylation procedures. this information, in combination with lectin reactivity patterns for the glycoproteins bound to nitrocellulose, has made it possible to define specific structural featu ... | 1986 | 3796628 |
| evidence for the glycoprotein nature of vitamin k-dependent carboxylase from rat liver. | the ability of the rat liver microsomal vitamin k-dependent carboxylase and microsomal precursors of prothrombin and other vitamin k-dependent proteins to bind to lectin gels has been determined. under denaturing conditions which dissociate precursor substrates from the carboxylase enzyme, prothrombin precursors and microsomal proteins labeled in gamma-carboxyglutamate residues with [14c]bicarbonate were nearly quantitatively bound to concanavalin a gels. when lentil lectin gels were used, only ... | 1987 | 3801513 |
| morphology of seedling lentil (lens culinaris medic., fabaceae) as influenced by light intensity and heredity. | lentil seedlings (lens culinaris medic.) were grown out-of-doors, in a shaded greenhouse or in a controlled environment chamber (15 degrees c, 14 hr day under a bank of incandescent and fluorescent lights). bright light promoted branching of seedlings and inhibited internode elongation. internodes formed after transfer of plants from high to low or from low to high light intensity were like those grown in continuous high or low light intensity. branching was enhanced following transfer from high ... | 1986 | 3803992 |
| lentil lectin inhibits cells producing graft-versus-host reaction but does not suppress hematopoietic stem cells in mice. | treatment of mouse donors or recipients of allogeneic spleen cells with repeated injections of lentil lectin markedly suppressed regional and systemic graft-versus-host reactions. the lectin acted selectively on lymphoid cells; hematopoietic stem cells were largely left unaffected. repeated administration of the lectin decreased the number of lymphocytes and red blood cells and the amount of hemoglobin; other white blood cells increased in number. | 1986 | 3804372 |
| differential redistribution of lectin receptor classes on clonal rat myotubes and myoblasts. | to evaluate the relative mobilities of cell surface glycoconjugates during myogenesis we have studied the redistribution of fluorescein-conjugated plant lectins on l6 rat myogenic cells. previous experiments had demonstrated that the receptors for the lectins soybean agglutinin (sba), wheat germ agglutinin, concanavalin a and lens culinaris agglutinin all were relatively uniformly distributed on both myoblasts and myotubes, and that sba receptors were capable of rapid redistribution on myotubes ... | 1986 | 3805140 |