Publications

mechanisms of neutrophil recruitment to the lung by grain dust exposure.	inhalation of grain dusts can cause symptoms of both acute and chronic bronchitis, which has been associated with a neutrophilic inflammatory response in the lung. since this influx of neutrophils may potentially result in bronchial injury, mechanisms of neutrophil recruitment to grain dust were examined. sterile, aqueous grain dust extracts were prepared from settled dusts of grain sorghum, corn, oats, and soybeans. the extracts were evaluated for their ability to directly attract human neutrop ...	1988	3059885
nickel as a micronutrient element for plants.	the detrimental effects of excessive ni on plant growth have been well known for many years. more recent evidence indicates that ni is required in small amounts for normal plant growth and development. ni is an essential component of urease in plants and microorganisms. a deficiency of ni in plants is reported to result in necrotic lesions in leaves in response to toxic accumulations of urea. urease plays an essential role in mobilization of nitrogenous compounds in plants, a process that is esp ...	1988	3076427
flavones and isoflavones as inducing substances of legume nodulation.	rhizobia are soil bacteria that can form symbiotic associations with leguminous plants leading to the fixation of atmospheric nitrogen to ammonia which the plant can use. this is an interaction which involves the exchange of many signals between the plant and the bacterium. to start this interaction, rhizobia have adapted to use flavonoid compounds, released by the plant root, as part of a regulatory system to initiate the transcription of their infection (nodulation, nod) genes. the development ...	1988	3076431
aflatoxin contamination in soybeans: role of proteinase inhibitors, zinc availability, and seed coat integrity.	soybean trypsin inhibitors are thought to ward off pathogens. studies with aflatoxigenic strains of aspergillus flavus and a. parasiticus, frequent soybean contaminants, revealed that trypsin inhibitors do not affect the growth of these fungi and aflatoxin production. further, the availability of zinc, an essential mineral for aflatoxin synthesis that was thought to explain increased aflatoxin accumulation in cooked compared with raw soybeans, was shown to decrease upon cooking. seed coat integr ...	1986	3089160
lectin binding patterns in amphibian epidermis.	a battery of 6 different horseradish peroxidase conjugated lectins has been employed for structural localization of glycoconjugates in amphibian epidermis. lens culinaris (lca) lectin stained the basal membrane and gave no significant reaction on the epidermal layers. canavlia ensiformis (con a) and griffonia simplicifolia ii (gs ii) lectins bound the keratinocyte cytoplasm and the basal membrane as well. ulex europaeus i (uea i) lectin had only reactivity with flask cells. griffonia simplifolia ...	1987	3105215
analysis of the stereochemistry of lipoxygenase-derived hydroxypolyenoic fatty acids by means of chiral phase high-pressure liquid chromatography.	a chiral phase hplc method was developed for the simultaneous determination of the positional and optical isomers of the lipoxygenase-derived hydroxypolyenoic fatty acids. with a bakerbond chiral phase hplc column (dinitrobenzoyl phenylglycine as chiral phase) the positional and optical isomers of the reduced dioxygenation products (by triphenylphosphine or borohydride) of linoleic acid and arachidonic acid were separated after methylation of the carboxylic groups. no cumbersome chemical derivat ...	1987	3105349
primary structure of soybean lipoxygenase-1.	the primary structure of lipoxygenase-1 from soybeans has been determined. the results were deduced from the nucleotide sequence of the complete cdna for isozyme lipoxygenase-1 and were confirmed by edman sequencing of selected peptides obtained by cnbr cleavage and supported by carboxyl-terminal sequencing of the intact protein and by partial peptide analysis employing fast atom bombardment-mass spectroscopy. the enzyme has a molecular weight of 94,038 based on its content of 838 amino acids.	2011	3112136
the reactive sites of proteinase inhibitors from erythrina seeds.	although the kunitz-type proteinase inhibitors from the seeds of various erythrina species have similar molecular weights (approximately 20,000), and share many other chemical characteristics, they could nevertheless be divided into three groups on the basis of their relative abilities to inhibit chymotrypsin, trypsin and tissue plasminogen activator. group a inhibitors were relatively specific for chymotrypsin; they were poor inhibitors of trypsin and had no apparent effect upon tissue plasmino ...	1987	3114019
structure and evolution of the 4.5-5s ribosomal rna intergenic region from glycine max (soya bean).	the nucleotide sequence for the 4.5-5s ribosomal dna region from the chloroplastids of soya beans was determined as the basis of further comparative studies on the structure and evolution of this intergenic region. comparisons with other plant sequences as well as equivalent sequences in eubacteria suggest that the longer internal transcribed spacer regions of plants have evolved, at least in part, by dna sequence duplications and that the presence of the 4.5s rrna in chloroplast may result from ...	1987	3116503
effects of ca2+ on phytoalexin induction by fungal elicitor in soybean cells.	a glucan elicitor from the cell walls of the fungus phytophthora megasperma f.sp. glycinea caused increases in the activities of the phytoalexin biosynthetic enzymes, phenylalanine ammonia-lyase and chalcone synthase, and induced the production of the phytoalexin, glyceollin, in soybean (glycine max) cell suspension cultures when tested in culture medium containing 1.2 mmol/liter ca2+. removal of extracellular ca2+ by treatment with ethylene glycol bis(beta-aminoethyl ether)-n, n'-tetraacetic ac ...	1987	3116938
detection of surface carbohydrates on pneumocystis carinii by fluorescein-conjugated lectins.	lectins react with a wide range of different carbohydrates (table 1). even so-called monospecific anti-h(o) lectins from lotus tetragonolobus, ulex europaeus, and anguilla anguilla react not only with the anti-h determinant but also with several fucosylated carbohydrates. consequently, the type of lectin receptor existing on the surface of pneumocystis carinii should be determined, because only a carbohydrate analysis can demonstrate the kind of carbohydrates which exist on the cell surface of t ...	1987	3125541
localization of specific carbohydrate configurations in the hemophilic synovial membrane.	fluorescein isothiocyanate-conjugated lectins include: concanavalin a (con-a), dolichos biflorus agglutinin (dba), griffonia simplicifolia-i (gs-i), griffonia simplicifolia-ii (gs-ii), arachis hypogaea agglutinin (pna), maclura pomifera agglutinin (mpa), ricinus communis agglutinin-i (rca-i), glycine max agglutinin (sba), ulex europaeus agglutinin-i (uea-i), and wheat germ agglutinin (wga). these lectins were used to histochemically demonstrate lectin bindings on hemophilic synovial membrane. gs ...	1988	3130210
ketones as electrophilic substrates of lipoxygenase.	the rate-limiting step of the lipoxygenase reaction involves the abstraction of a hydrogen from the methylene carbon of a 1,4-diene. one possibility for the mechanism of the enzyme is the abstraction of this hydrogen as a proton to generate a carbanionic intermediate or transition state. in order to investigate this possibility, 5-, 8-, 12-, and 15-hydroxy-eicosatetraenoic acid were oxidized to the corresponding ketones and these ketones were assayed as substrates of the 5-, 12-, and 15-lipoxyge ...	1988	3135805
lipoxygenase catalyzed oxygenation of hydroxy fatty acids to lipoxins.	the pure lipoxygenases from rabbit reticulocytes and soybeans convert a variety of substrates (arachidonic acid, 15-hpete, 15-hete, 5-hete, various dihete isomers) to trihydroxy eicosanoids containing a conjugated tetraene system (lipoxins). in general, the methyl esters are better substrates for lipoxin formation than are the free acids. lipoxygenase inhibitors (5,8,11,14-eicosatetraynoic acid, nordihydroguaiaretic acid) strongly inhibit the lipoxin formation. the complete stereochemistry of th ...	1988	3138902
[dynamics of respiration and the microbial biomass in soil supplemented with straw from corn and soybeans].	soil organic matter level has an important influence on land productivity. this level depends upon the efficiency in partitioning carbon from plant debris into co2 and humified substances by microorganisms. the c/n ratio of the residues is one of the factors that affects this process. to study its influence on the growth and activity of the microflora, a soil from the corn region of buenos aires was supplemented with different amounts of straw from corn and soybean, and respiration and microbial ...	1988	3140295
effects of alpha-tocopherol, its carboxylic acid chromane compound and two novel antioxidant isoflavanones on prostaglandin h synthase activity and autodeactivation.	the natural antioxidant alpha-tocopherol has repeatedly been described to inhibit platelet aggregation and thromboxane formation, whereas its influence on prostaglandin h synthase in vivo and in vitro is a matter of controversy. in the present study the effects of different antioxidative compounds on ram vesicular gland microsomal prostaglandin h synthase activity were investigated in vitro: d,l-alpha-tocopherol, its carboxylic acid chromane compound (trolox), phytol, alpha-tocopherol-acetate an ...	1988	3148864
lectin histochemistry of the thyroid gland.	the authors carried out a histochemical study with lectins (ulex europaeus agglutinin-i [uea-i], triticum vulgaris [wga], glycine max [sba], dolichos biflorus [dba], and arachis hypogaea [pna]) in different thyroid gland conditions (17 benign nodular goiters, three diffuse hyperplasias, five hashimoto's thyroiditis, 20 follicular adenomas, 14 well-differentiated papillary carcinomas, five well-differentiated follicular carcinomas, and 30 normal thyroids) in order to determine if specific lectin ...	1988	3179951
effect of lupines, a protein-rich legume, on iron absorption.	the absorption of iron from lupines, a protein-rich legume, was assessed in 35 parous indian women. iron bioavailability was shown to be as low for lupines as soybeans (geometric mean absorptions 0.9 and 1.7 per cent respectively, p less than 0.005). the addition of 30 mg of ascorbic acid moderately improved the geometric mean iron absorption from 1.0 to 5.3 per cent (p less than 0.0001), and 60 mg ascorbic acid from 0.7 to 6.9 per cent (p less than 0.0001). the phytate and polyphenol content of ...	1988	3181102
tracheobronchial epithelium of the sheep: iv. lectin histochemical characterization of secretory epithelial cells.	conventional histochemical characterization of the mucus secretory apparatus is often difficult to reconcile with the biochemical analysis of respiratory secretions. this study was designed to examine the secretory glycoconjugates in airways using lectins with biochemically defined affinities for main sugar residues of mucus. we used five biotinylated lectins--dba (dolichos biflorus) and sba (glycine max) for n-acetyl galactosamine (galnac), bsa i (bandeiraea simplicifolia) and pna (arachis hypo ...	1988	3189886
molybdate transport by bradyrhizobium japonicum bacteroids.	bacteroid suspensions of bradyrhizobium japonicum usda 136 isolated from soybeans grown in mo-deficient conditions were able to transport molybdate at a nearly constant rate for up to 1 min. the apparent km for molybdate was 0.1 microm, and the vmax was about 5 pmol/min per mg (dry weight) of bacteroid. supplementation of bacteroid suspensions with oxidizable carbon sources did not markedly increase molybdate uptake rates. anaerobically isolated bacteroids accumulated twice as much mo in 1 h as ...	1988	3192511
[experimental 3-month study of safety of textured soybeans becsei].	the authors conducted a three-month safety test of a textured soy (ts) product manufactured by the agricultural plant, boly. young male and female rats received semisynthetic diets containing 8, 18 and 38% of ts protein or casein supplemented with methionine, as the only source of protein. the effect of the diet containing 18% of ts protein on the growth, on certain parameters of protein and fat metabolism and on the zinc and copper status corresponded to that produced by 18% of casein supplemen ...	1988	3206854
wheat germ splicing endonuclease is highly specific for plant pre-trnas.	intron-containing pre-trnas from organisms as different as yeast, nicotiana, xenopus and man are efficiently spliced and processed in a hela cell extract. they are also correctly processed in a wheat germ extract; however, the intron is removed only from the tobacco pre-trna. to determine whether plant pre-trna introns have any specific structural and/or sequence feature we have cloned two intron-containing trnatyr genes from the plant arabidopsis. comparison of these genes, of the nicotiana trn ...	1988	3208752
mutagenic and anti-mutagenic properties of meju and other korean food products from fermented soybeans.		1988	3218254
primary structure and promoter analysis of leghemoglobin genes of the stem-nodulated tropical legume sesbania rostrata: conserved coding sequences, cis-elements and trans-acting factors.	the primary structure of a leghemoglobin (lb) gene from the stem-nodulated, tropical legume sesbania rostrata and two lb gene promoter regions was analysed. the s. rostrata lb gene structure and lb amino acid composition were found to be highly conserved with previously described lb genes and lb proteins. distinct dna elements were identified in the s. rostrata lb promoter regions, which share a high degree of homology with cis-active regulatory elements found in the soybean (glycine max) lbc3 p ...	1988	3237206
identification and isolation of methionine-cysteine rich proteins in soybean seed.	we recently developed a method to identify methionine-containing proteins and quantitate their methionine contents. we applied this method to soybeans and identified relatively methionine-rich proteins (mrp) among the albumins. by acidic methanol extraction of the albumins, we obtained a group of low molecular weight methionine-cysteine rich proteins (mcrp) that analyzed 4.0% methionine and 8.8% cysteine. mcrp made up 1-2% of the total protein in soybeans. reversed-phase hplc purification of mcr ...	1988	3237631
expression of glycoconjugates on normally developing and immunologically impaired hymenolepis diminuta.	the carbohydrates on the surface of hymenolepis diminuta were analyzed with gold-labelled lectins, and it was found that the surface coat of the anterior body differs from that of the strobila in its lectin-binding properties. binding sites for lectins from abrus precatorius (apa), arachis hypogaea (pna), glycine max (sba) and for wheat germ agglutinin (wga) and succinylated wga were located on the scolex and strobilation zone. lectin-gold particles attached mainly to the electron-dense spines. ...	1988	3237676
a small scale procedure for the isolation of transport competent vesicles from plant tissues.	a microscale method for the isolation of selectively sealed microsomal membrane fractions from plant tissue is presented. the method is based on differential centrifugation in a table top microcentrifuge to accommodate small sample size (10-25 g tissue) and the addition of ki or kcl in the homogenization medium for isolating selectively sealed plasma membrane or tonoplast vesicles. this microscale procedure was found to be useful in isolating membranes from red beet (beta vulgaris) storage tissu ...	1988	3239758
purification and characterization of locusta migratoria chymotrypsin.	a chymotrypsin-like enzyme (ctle) was isolated from the digestive tract of the african migratory locust locusta migratoria migratorioides by ion-exchange chromatography on diethylaminoethyl (deae) cellulose followed by affinity chromatography on phenylbutylamine (pba) sepharose. the purity and homogeneity of ctle have been shown by sds-page and on cellulose acetate strips. the enzyme has a molecular weight of 24,000, determined by sds-page and on a sephadex g-75 calibrated column. it has an isoe ...	1988	3246483
[saponin and sapogenol. xliv. soyasaponin composition in soybeans of various origins and soyasaponin content in various organs of soybean. structure of soyasaponin v from soybean hypocotyl].		1988	3249299
lectin histochemistry on mucous substances of the taste buds and adjacent epithelia of different vertebrates.	in the present study carbohydrate residues in taste buds (tbs) and adjacent epithelial formations of a teleostean fish, a frog and the rabbit were detected by means of lectin histochemistry. biotinylated lectins from pisum sativum (psa), arachis hypogaea (pna), dolichos biflorus (dba), triticum vulgaris (wga and succinylated wga), glycine max (sba) and ulex europaeus (uea i) have been applied. the lectins were bound to an avidin-biotin-peroxidase-complex (abc) and visualized by diaminobenzidine/ ...	1988	3259218
pyrene acts as a cocarcinogen with the carcinogens benzo[a]pyrene, beta-propiolactone and radiation in the induction of malignant transformation in cultured mouse fibroblasts; soybean extract containing the bowman-birk inhibitor acts as an anticarcinogen.	pyrene was found to act as a cocarcinogen in the induction of transformation of cultured balb/c3t3 cells by three different types of carcinogens: a direct acting chemical carcinogen, beta-propiolactone, a chemical carcinogen requiring metabolic activation, benzo[a]pyrene, and a physical carcinogen (60co) gamma radiation. since pyrene enhanced transformation in vitro by approximately the same amount for all the carcinogens tested, these results suggest that the carcinogenic action of pyrene is no ...	1986	3267443
carbohydrate-binding specificities of five lectins that bind to o-glycosyl-linked carbohydrate chains. quantitative analysis by frontal-affinity chromatography.	the carbohydrate-binding specificities of lectins purified from agaricus bisporus (aba-i), arachis hypogaea (pna), bauhinia purpurea (bpa), glycine max (sba), and vicia villosa (vva-b4) have been studied by affinity chromatography on columns of the immobilized lectins, and quantitatively analyzed by frontal affinity chromatography. these five lectins could be classified into two groups with respect to their reactivities with typical mucin-type glycopeptides, beta-d-galp-(1----3)-alpha-d-galpnac- ...	1988	3274131
high and low inhibitor soybean meals affect human duodenal proteinase activity differently: in vitro comparison of proteinase inhibition.	the purpose of the study was to investigate the in vitro inhibition of the proteolytic activity in samples of duodenal juice obtained during instillation of raw soybeans in humans. the results suggested the presence of an inhibitor-resistant trypsin. practically no inhibition was obtained with phenylmethylsulfonyl fluoride, aprotinin, human alpha 1-antitrypsin or lima bean trypsin inhibitor. specificity against synthetic substrates was different from that obtained in the absence of raw soybeans. ...	1988	3282043
the toxicologic effects of the carbamate insecticide aldicarb in mammals: a review.	aldicarb, 2-methyl-2-(methylthio)propionaldehyde-o-methylcarbamoyloxime, is an oxime carbamate insecticide manufactured by the union carbide corporation and sold under the trade name temik. it is a soil-applied systemic pesticide used against certain insects, mites, and nematodes, and is applied below the soil surface for absorption by plant roots. it is generally applied to the soil in the form of 5, 10, or 15% granules, and soil moisture is essential for the release of the toxicant. uptake by ...	1987	3304999
distribution of k vitamins (phylloquinone and menaquinones) in human placenta and maternal and umbilical cord plasma.	it is a well-known fact that neonatal and infantile vitamin k deficiencies cause melena neonatorum and intracranial hemorrhagic disorders. these disorders occur more frequently in breast-fed babies than in bottle-fed babies, for reasons that are still obscure. endogenous concentrations of k vitamins (phylloquinone and menaquinones) in human placentas and in paired specimens of maternal and umbilical cord plasma were detected by high-performance liquid chromatography with fluorometric detection a ...	1988	3348316
proline metabolism in n2-fixing root nodules: energy transfer and regulation of purine synthesis.	n2-fixing root nodules of soybean (glycine max l. merr.) convert atmospheric n2 to ammonia(um) in an energy-intensive enzymatic reaction. these nodules synthesize large quantities of purines because nitrogen fixed by bacteria contained within this tissue is transferred to the shoots in the form of ureides, which are degradation products of purines. in animal systems, it has been proposed that proline biosynthesis by pyrroline-5-carboxylate reductase (p5cr) is used to generate the nadp+ required ...	1988	3353366
nutritive value of whole soybeans fermented with aspergillus oryzae or rhizopus oligosporus as evaluated by neonatal pigs.	two experiments were conducted to evaluate the nutritive effects of heated whole soybeans fermented with the mold aspergillus oryzae (aos) or rhizopus oligosporus (ros) in diets fed to neonatal pigs from 1 to 22 d of age. in experiment 1, either soybean meal or heated whole soybeans fermented with ros replaced 75% of the dried skim milk protein. the average daily gain (adg) and gain-feed (g-f) ratio of pigs fed the diet containing 100% milk protein were greater than those of pigs fed the diets c ...	1988	3357058
structure and activity of trypsin in reverse micelles.	the kinetic properties of trypsin have been studied in reverse micelles formed by two surfactant systems, namely bis(2-ethylhexyl) sodium sulfosuccinate (aot) in isooctane, and hexadecyltrimethyl ammonium bromide (ctab) in chloroform/isooctane (1:1, by vol.). three substrates have been used, namely n alpha-benzoyl-l-arg ethyl ester, n alpha-benzoyl-l-phe-l-val-l-arg p-nitroanilide (bzphevalarg-nh-np) in aot and n alpha-benzyloxycarbonyl-l-lys p-nitrophenyl ester (zlyso-np) in ctab. one of the ma ...	1988	3360018
purification and properties of s-adenosyl-l-methionine:nicotinic acid-n-methyltransferase from cell suspension cultures of glycine max l.	a soluble enzyme which catalyzes the transfer of the methyl group from s-adenosyl-l-methionine to the nitrogen atom of pyridine-3-carboxylic acid (nicotinic acid) could be detected in protein preparations from heterotrophic cell suspension cultures of soybean (glycine max l.). enzyme activity was enriched nearly 100-fold by ammonium sulfate precipitation, gel filtration, and ion-exchange chromatography to study kinetic properties. s-adenosyl-l-methionine:nicotinic acid-n-methyltransferase (ec 2. ...	1988	3364975
precipitation of galactose-specific lectins by complex-type oligosaccharides and glycopeptides: studies with lectins from ricinus communis (agglutinin i), erythrina indica, erythrina arborescens, abrus precatorius (agglutinin), and glycine max (soybean).	we have recently demonstrated that certain oligomannose and bisected hybrid type glycopeptides and bisected complex type oligosaccharides are bivalent for binding to concanavalin a and can precipitate the lectin [bhattacharyya, l., ceccarini, c., lorenzoni, p., & brewer, c.f. (1987) j. biol. chem. 262, 1288-1293; bhattacharyya, l., haraldsson, m., & brewer, c.f. (1987) j. biol. chem. 262, 1294-1299]. the present results show that tri- and tetraantennary complex type oligosaccharides containing n ...	1988	3365364
lactational response to soybean meal, heated soybean meal, and extruded soybeans with ruminally protected methionine.	seventy-three high producing holstein cows were arranged in a 3 x 2 factorial to evaluate three protein supplements (soybean meal, heat-treated soybean meal, and extruded blend of soybeans and soybean meal) without or with 15 g/head/d of ruminally protected dl-methionine during wk 4 through 16 postpartum. total mixed diets contained (dm basis) 30% corn silage, 15% alfalfa hay, and 55% of the respective concentrate mix. milk production was higher when cows were fed either heated soybean product i ...	1988	3372801
influence of jet-sploding and extrusion on ruminal and intestinal disappearance of canola and soybeans.	in situ rumen degradation and intestinal digestibility of dm and cp of jet-sploded and extruded protein sources were estimated, using two ruminally and duodenally cannulated lactating holstein cows, in two experiments. one-gram samples of ground (1 mm) whole canola seed, canola meal, extruded canola meal, jet-sploded whole canola seed, protec, soybean meal and extruded soybean meal (experiment 1), and whole canola seed jet-sploded at temperatures ranging from 116 to 177 degrees c (experiment 2) ...	1988	3372817
allergenicity of major component proteins of soybean determined by enzyme-linked immunosorbent assay (elisa) and immunoblotting in children with atopic dermatitis and positive soy challenges.	recent studies have implicated soybeans as one of the major food allergens in children. serum from eight patients with atopic dermatitis and positive double-blind, placebo-controlled food challenges to soy were used to investigate allergen-specific ige and igg antibody to soy protein and its fractions. elisa and immunoblotting techniques were used for this purpose. a crude soy extract, as well as the 7s, 11s, and whey fractions, were isolated and purified for the in vitro studies from commercial ...	1988	3379225
purification and characterization of chalcone isomerase from soybeans.	chalcone isomerase from soybean has been purified 11,000-fold over the crude extract. the purification procedure features pseudo-affinity chromatography on an amicon matrex orange a column with selective elution by a product of the enzymatic reaction. the purified enzyme is greater than 99.5% pure and possesses a specificity activity of 340 iu/mg, which is 520-fold greater than previously reported. the apparent molecular weight of the chalcone isomerase is 24,000 as determined from sodium dodecy ...	1988	3384815
gas chromatographic screening method for t-2 toxin, diacetoxyscirpenol, deoxynivalenol, and related trichothecenes in feeds.	a gas chromatographic method for screening trichothecene mycotoxins in feeds is described. feed is extracted with acetonitrile-water, and the toxins are purified with charcoal-alumina-celite, florisil, and silica mini-columns. deoxynivalenol (don), nivalenol (niv), diacetoxyscirpenol (das), t-2 toxin, and their fungal metabolites are hydrolyzed to their corresponding parent alcohols (don, niv, scirpentriol, or t-2 tetraol) by alkaline hydrolysis. after derivatization to their pentafluoropropiony ...	2016	3391946
glycoconjugate expression in human renal oncocytomas. a lectin histochemical study.	a panel of lectins chosen for its specificity for segments of the nephron was used in an avidin-biotin-peroxidase reaction to stain 13 human renal oncocytomas and a specimen of oncocytomatosis. the lectins were derived from lotus tetragonolobus, glycine max, dolichos biflorus, and arachis hypogaea. two patterns predominated: one of staining with the lectins of d biflorus and g max, suggesting distal tubular or collecting-duct differentiation, and one of staining with the lectin of l tetragonolob ...	1988	3395218
high-affinity binding of fungal beta-glucan fragments to soybean (glycine max l.) microsomal fractions and protoplasts.	we have recently reported the existence of binding sites in soybean membranes for a beta-glucan fraction derived from the fungal pathogen phytophthora megasperma f. sp. glycinea, which may play a role in the elicitor-mediated phytoalexin response of this plant [schmidt, w. e. & ebel, j. (1987) proc. natl acad. sci. usa 84, 4117-4121]. the specificity of beta-glucan binding to soybean membranes has now been investigated using a variety of competing polyglucans and oligoglucans of fungal origin. p ...	1988	3402458
utilization by growing and finishing pigs of raw soybeans of low kunitz trypsin inhibitor content.	three trials were conducted to compare acceptance and utilization by growing and finishing pigs of diets containing supplemental protein from either heated, solvent-extracted soybean meal (sbm), raw low-kunitz trypsin inhibitor soybean (lt) or raw commercially grown williams cultivar soybean with high kunitz trypsin inhibitor content (ht). in trial 1, 36 crossbred pigs, averaging 7 kg in weight, were fed 1) corn-sbm, 2)corn-lt or 3) corn-ht diets for 28 d. diets were formulated to be isolysinic ...	1988	3403398
an evaluation of a dry-fat product as a source of supplemental energy in pig diets.	three feeding trials (278 pigs) and one balance trial (14 pigs) were conducted to evaluate acceptance and utilization of diets containing a dry-fat product containing 90% fat. the fat was a mixture of equal parts vegetable and animal fats. in trial 1, 4-wk-old pigs fed diets with 5% dry-fat added had average daily gain (adg), average daily feed intake (adfi) and gain/feed (g/f) values equal to pigs fed the diet containing 4.5% liquid fat, the unprocessed equivalent to the dry-fat. values for g/f ...	1988	3403400
carotenoid composition of serum and egg yolks of hens fed diets varying in carotenoid composition.	high performance liquid chromatography of yolks of hens fed a diet based on yellow corn, alfalfa, and soybeans revealed over 20 cartenoids. lutein, lutein monester, lutein diester, 3'-oxolutein, cryptoxanthin, zeaxanthin, beta-carotene, and zeacarotene were identified by their retention times, visible absorption spectra, behavior on saponification, and their presence or absence when lutein was the primary carotenoid fed. three weeks after placing the hens on a white corn-soy-based diet supplemen ...	1988	3405937
the sequence of a gene encoding convicilin from pea (pisum sativum l.) shows that convicilin differs from vicilin by an insertion near the n-terminus.	the sequence of a gene encoding convicilin, a seed storage protein in pea (pisum sativum l.), is reported. this gene, designated cvca, is one of a sub-family of two active genes. the transcription start of cvca was mapped. convicilin genes are expressed in developing pea seed cotyledons, with maximum levels of the corresponding mrna species present at 16-18 days after flowering. the gene sequence shows that convicilin is similar to vicilin, but differs by the insertion of a 121-amino-acid sequen ...	1988	3415641
daily variations in the sensitivity of soybean seedlings to low temperature.	every 4 hr during a time span of 32 hr and of 76 hr a different group of soybean seedlings [glycine max (l.) merr., cv. corsoy] maintained under a regime of 16 hr of light followed by 8 hr of darkness was exposed to -10 degrees c for 4 min. extent of sensitivity to low temperature was evaluated approximately 10 days after exposure to freezing temperature by determining the weight of the plants and chlorophyll content of the cotyledons. the amount of sensitivity to low temperature was related to ...	1987	3435931
effect of soybean lipid on growth and ruminal nitrogen metabolism in cattle fed soybean meal or ground whole soybeans.	three experiments were conducted to determine the effect of soybean lipid on ruminal proteolysis of soybean meal (sbm) and ground whole soybeans (gsb). experiment 1 was a 92-d growth experiment using 120 calves (206 kg) allotted to 12 pens of 10 calves each. three replicate pens were assigned to each of the treatment supplements: low sbm (lsbm), low gsb (lgsb), high sbm (hsbm) and high gsb (hgsb). calves received ad libitum amounts of corn silage top-dressed with the respective supplement (.81 k ...	1987	3443588
ultrastructural studies of the liver and skeletal muscles of rats fed on soybeans.		1987	3448423
inheritance and linkage analysis of phosphoglucose isomerase isozymes in soybeans.		1987	3450277
influence of diets on growth in the rat.	the proportions of amino acids required for growth and maintenance are substantially different. alterations in nutrient profiles of soybeans due to hydrothermic alkaline processing have been reported. the purpose of the present work was to investigate the effects of amino acid losses during hydrothermic alkaline processing of soybeans on growth in the rat. the protein sources of diets a, b and c were untreated soybean cotyledones, hydrothermic alkaline processed soybeans and casein, respectively ...	1987	3452459
in vitro effect of soybean (glycine max l. merr)lectin on lipid peroxidation in pyridoxine deficient albino rats.		1987	3452602
genetic linkage of a leucine aminopeptidase locus with the kunitz trypsin inhibitor locus in soybeans.	the soybean (glycine max (l.) merrill) seed leucine aminopeptidase locus (lap1) was found to be linked to the kunitz trypsin inhibitor locus (ti) with a recombination frequency of 15.3 percent +/- 0.9 percent. the two loci are in linkage group 9. both lap1 and ti loci are inherited independently of the flower color locus (w1) in linkage group 8.	2012	3458809
organization and characterization of genes essential for symbiotic nitrogen fixation from bradyrhizobium japonicum i110.	a total of 96 independent tn5 insertions within a 39-kilobase-pair (kbp) segment of chromosomal dna containing the three structural genes for nitrogenase (nifh, nifd, and nifk) from bradyhizobium japonicum i110 were obtained in escherichia coli and transferred to the wild-type strain by marker exchange. individual transconjugants containing a tn5 insertion were inoculated onto glycine max cv. wilkin (soybeans) and analyzed for their effect on symbiotic nitrogen fixation. in addition to the three ...	1986	3462181
variability in molybdenum uptake activity in bradyrhizobium japonicum strains.	twenty naturally occurring strains of bradyrhizobium japonicum in 11 serogroups were screened for the ability to take up mo as bacteroids from soybean root nodules. the strains varied greatly in their ability to take up mo in a 1-min period. the best strain was usda 136, which had an mo uptake activity of almost 3.0 pmol/min per mg of bacteroid (dry weight). in contrast, the poorest strain, usda 62, had an mo uptake activity of 0.35 pmol of mo per min per mg of bacteroid. there were similarities ...	1987	3473064
lectin histochemical study on the infraorbital gland of the japanese serow (capricornis crispus).	histology and lectin histochemistry were performed in the infraorbital gland of the japanese serow. the gland is composed of glandular tissues and a pouch filled with the secretion. the tissues consist of an inner layer of sebaceous glands and an outer layer of apocrine glands. the male sebaceous layer is made up of the ordinary type, whereas the female's layer consists of the ordinary and modified types. in the apocrine gland stained with arachis hypogaea (pna), nine different patterns of gland ...	1987	3506377
development and evaluation of a low-cost amaranth (amaranthus cruentus) containing food for preschool children.	a powdered blend for feeding preschool children was prepared from whole amaranth (amaranthus cruentus) seeds, pearled oats, soybeans, sucrose and vegetable oil. the blend was similar in proximal chemical analysis and total caloric content, and identical in calorie distribution and vitamin and mineral patterns to a soy/oats infant formula previously developed. the amaranth blend equalled the fao/who 1973 children's pattern in tryptophan and exceeded this pattern, as well as the soy/oats formula i ...	1987	3506402
lectin binding sites on human endocervix: a comparison with secretory and proliferative endometrium.	endocervix and corresponding endometrium of women of reproductive age were studied histochemically with 13 fluorescein isothiocyanate-labeled lectins to delineate the differences between the epithelial cells in two anatomical sites. lectin from maclura pomifera (mpa), ulex europaeus (uea-i), glycine max (sba), and vicia villosa (vva) bound only to endocervical epithelium and were the only four lectins that distinguished endocervical from endometrial epithelium. these differences were independent ...	1986	3526979
endogenous lectins from cultured soybean cells: isolation of a protein immunologically cross-reactive with seed soybean agglutinin and analysis of its role in binding of rhizobium japonicum.	incubation of rhizobium japonicum with the cultured soybean cell line sb-1, originally derived from the roots of glycine max, resulted in specific adhesion of the bacteria to the plant cells. this binding interaction appears to be mediated via carbohydrate recognition, since galactose can inhibit the heterotypic adhesion but glucose cannot. affinity chromatography, on a sepharose column derivatized with n-caproyl-galactosamine, of the supernatant fraction of a sb-1 cell suspension after enzymati ...	1986	3528167
fluorescent visualization of binding and internalization of the anticarcinogenic bowman-birk type protease inhibitors in transformed fibroblasts.	the bowman-birk protease inhibitors from soybeans and chick peas suppress in vitro malignant transformation. this fluorescent microscopy study is designed to visualize the cellular site of action of these protease inhibitors. binding and internalization of active protease inhibitors occur over a time course of 2 h. the rate and character of fluorescent micrographs obtained are compared to insulin as a positive control. internalization of both molecules is completely blocked at 4 degrees c. inter ...	1987	3545449
lectin binding patterns in developing rat colon.	to evaluate qualitative changes in brush-border and goblet cell glycoconjugates during colonic development, rhodamine-conjugated lectins were applied to tissue from fetal (18-22 day), suckling (1-20 day), and weanling (22-26 day) rats. tissue from the proximal and distal colon of each animal was incubated with triticum vulgaris, arachis hypogaea, glycine max, or ulex europeus agglutinin i. formalin-fixed paraffin sections and unfixed frozen sections were coded, read blindly, and graded from nega ...	1987	3557008
protected methionine supplementation with extruded blend of soybeans and soybean meal for dairy cows.	methionine may be the first amino acid limiting milk production in early lactation cows. to evaluate this further, 23 high producing holstein cows (9 multiparous and 14 primiparous) were fed an extruded blend of soybeans and soybean meal (40:60) without or with 15 g of added dl-methionine as 50 g of ruminally protected methionine product during wk 4 to 16 postpartum. cows were fed a 15.8% crude protein total mixed ration consisting of 30% (dry basis) corn silage, 15% alfalfa hay, and 55% concent ...	1987	3571638
aberrant, canavanyl protein formation and the ability to tolerate or utilize l-canavanine.	l-canavanine, 2-amino-4-(guanidinooxy)butyric acid, and l-arginine incorporation into de novo synthesized proteins was compared in six organisms. utilizing l-[guanidinooxy14c]canavanine and l-[guanidino14c]arginine at substrate saturation, the canavanine to arginine incorporation ratio was determined in de novo synthesized proteins. caryedes brasiliensis and sternechus tuberculatus, canavanine utilizing insects; canavalia ensiformis, a canavanine storing plant; and to a lesser extent heliothis v ...	1987	3582574
a locus encoding host range is linked to the common nodulation genes of bradyrhizobium japonicum.	by using cloned rhizobium meliloti, rhizobium leguminosarum, and rhizobium sp. strain mpik3030 nodulation (nod) genes as hybridization probes, homologous regions were detected in the slow-growing soybean symbiont bradyrhizobium japonicum usda 110. these regions were found to cluster within a 25-kilobase (kb) region. specific nod probes from r. meliloti were used to identify noda-, nodb-, nodc-, and nodd-like sequences clustered on two adjacent hindiii restriction fragments of 3.9 and 5.6 kb. a 7 ...	1987	3584066
endogenous lectin from cultured soybean cells. chemical characterization of the lectin of sb-1 cells.	a lectin has been identified in the cell line, sb-1, originally derived from the roots of glycine max. this lectin, which we shall refer to as sb-1 lectin, was isolated on the basis of its carbohydrate-binding activity (affinity chromatography on sepharose column derivatized with n-caproyl-galactosamine) and its immunological cross-reactivity (immunoblotting with rabbit antibodies directed against seed soybean agglutinin (sba]. sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) ...	1987	3584143
two types of high frequency oscillations in glycine max (l.) merr.	movements of the first trifoliolated leaf of glycine max (l.) merr. plants were monitored continuously for several weeks in a study that included 14 individual plants. two types of high frequency oscillations that had periods of 1 hr or less were superimposed on the circadian profile of eight of the 14 plants. the first type of leaf movement was found to be an ultradian oscillation having periods that ranged from 45 to 62 min. these oscillations started 2 to 5 hr after the beginning of the 15-hr ...	1987	3601976
cold sensitivity oscillations of young soybean plants.	every 4 hr, a different group of soybean seedlings (glycine max [l.] merr. cv. corsoy), maintained in a controlled environment chamber (ld 16:8), were briefly subjected to cold (-10 degrees c for 4 min). ten days after the beginning of the cold treatment, plants were harvested, weighed, and their cotyledons assayed for chlorophyll. in other experiments, potassium levels of the foliage were monitored. both the sensitivity of plants to cold and the potassium content of unifoliolate leaves not subj ...	1987	3601978
recovery of a soybean urease genomic clone by sequential library screening with two synthetic oligodeoxynucleotides.	we report the first isolation of a low-copy-number gene from a complex higher plant (soybean) genome by direct screening with synthetic oligodeoxynucleotide (oligo) probes. a synthetic, mixed, 21-nucleotide (nt) oligo (21-1) based on a seven amino acid (aa) sequence from soybean seed urease, was used to screen genomic libraries of soybean (glycine max [l.] merr.) in the lambda charon 4 vector. twenty homologous clones were recovered from a screen of 500,000 plaques. these were counterscreened wi ...	1987	3609752
amino acid sequence of a soybean (glycine max) seed polypeptide having a poly(l-aspartic acid) structure.	a polypeptide of mr 4400 was isolated from soybean (glycine max) seeds by extraction with 60% ethanol followed by ion-exchange and reverse-phase chromatography. the peptide contains an unusually high amount of aspartic acid residues (approximately 25%, and hence is designated as soybean aspartic acid-rich peptide). its complete primary structure was determined by conventional methods to be the following. ser-lys-trp-gln-his-gln-gln-asp-ser-cys-arg-lys-gln-leu-gln-gly-val-asn- leu-thr-pro-cys-glu ...	1987	3611081
transcriptional control of the inducible nitrate reductase isoform from soybeans.	the activity of the ph 7.5 nadh-linked nitrate reductase isoform from soybean seedlings is termed inducible. activity is present only in the leaves of seedlings which have been supplied nitrate. a cdna clone that encoded part of the mrna for squash nitrate reductase hybridized specifically with mrna for this inducible nitrate reductase isoform. nitrate induction resulted in an increase in the steady-state levels of mrna for this isoform after 24 hours, while the addition of glutamine to the nitr ...	1987	3619922
histochemical characterization of glycoproteins present in jejunal and colonic goblet cells of pigs on different diets. a biopsy study using chemical methods and peroxidase-labelled lectins.	we examined the glycoprotein composition of intestinal goblet cells in jejunal and colonic biopsies obtained from pigs on different diets. paraffin sections were stained both chemically and with the following horseradish-peroxidase conjugated lectins: canavalia ensiformis (con-a), limulus polyphemus (lpa), lotus tetragonolobus (lta), arachis hypogaea (pna), ricinus communis (rca1), glycine max (sba) and triticum vulgaris (wga). using chemical staining procedures, only small quantitative differen ...	1987	3624000
infrared spectroscopic evidence for a conformational alteration of plant plasma membranes upon exposure to the growth hormone analog, 2,4-dichlorophenoxyacetic acid.	infrared spectroscopy of highly purified fractions of plasma membrane vesicles from hypocotyls of etiolated soybean (glycine max l.) seedlings revealed changes in bands assigned to proteins and phospholipids upon exposure to the growth hormone analog, 2,4-dichlorophenoxyacetic acid (2,4-d). the changes included a concentration dependent broadening of amide i absorbance and a change in the absorbance ratios of amide i and amide ii indicative of a change in protein conformation. band broadening of ...	1987	3632681
isolation and characterization of ferritin from soyabeans (glycine max).	ferritin from the soyabean glycine max was isolated and characterized. the protein has many features in common with ferritin from mammalian systems, including extensive sequence homology, as determined by two-dimensional peptide mapping. no immunocross-reactivity between the plant and animal proteins was detected. the ferritin isolated by mgcl2 precipitation has a single subunit of 28 kda, whereas the ferritin remaining in the supernatant exhibits marked heterogeneity, with a main subunit of 22 ...	1987	3654637
a mechanism for the hypocholesterolaemic activity of saponins.	1. saponins are steroid or triterpene glycosides which occur in a number of important food plants, including such staples as soya beans (glycine max) and chickpeas (cicer arietinum). they are known to be hypocholesterolaemic. 2. some saponins form an insoluble complex with cholesterol which prevents its absorption from the small intestine. others cause an increase in the faecal excretion of bile acids, an indirect route for elimination of cholesterol. 3. we have investigated the effects of diffe ...	1986	3676181
high-mr glycoprotein profiles in human milk serum and fat-globule membrane.	gradient-polyacrylamide-gel electrophoresis of human milk serum separated three high-mr glycoprotein bands. the properties of the components corresponding to the three bands (tentatively termed 'components c, b and a' in their order of migration) were compared by staining with four monoclonal antibodies and lectins. components b and c both reacted with the four antibodies, but component a did not. components b and c were stained with peanut (arachis hypogaea) agglutinin (pna) and wheat (triticum ...	1986	3707520
microscopical comparison of cotton, corn, and soybean dusts.	specialized analytical methods are required for identification of components of agricultural dusts such as those generated in harvesting, transportation, storage, and processing of cotton, corn, and soybeans. the larger particles and trash components of the dusts can often be identified visually or with the aid of an optical microscope (om). the respirable portion of the dust, that which causes lung dysfunction, retains few structural features for identification. electron microscopy and x-ray mi ...	1986	3709475
cell surface carbohydrate of leishmania mexicana amazonensis: differences between infective and non-infective forms.	the cell surface carbohydrates of leishmania mexicana amazonensis (amastigotes and promastigotes, both infective and non-infective forms) were comparatively analyzed by agglutination assay employing 28 highly purified lectins, and by binding assay using 125i-labeled lectins. among the d-galnac binding lectins, bandeiraea simplicifolia-i, dolichos biflorus, phaseolus vulgaris and glycine max were highly specific for the amastigotes, while that from maclura aurantiaca selectively agglutinated prom ...	1986	3709546
determination of residues of alachlor and its metabolite in corn, soybeans, and potatoes as heptafluorobutyryl-2,6-diethylaniline.	alachlor [2-chloro-2',6'-diethyl-n-(methoxymethyl)acetanilide] was determined by hydrolysis to 2,6-diethylaniline with 6n hcl and quantitation of the heptafluorobutyryl derivative by gas chromatography with electron capture detection. 2,6-diethylaniline was isolated from the hydrolysate by steam distillation, derivatized with heptafluorobutyric anhydride, and the derivative was purified by chromatography on silicic acid with 60% toluene in hexane as eluant. recoveries from potatoes, soybeans, an ...	2012	3745082
modified clearing method to show sieve tubes in minor veins of leaves.	leaf samples of glycine max and numerous other dicotyledonous species were cleared by a common, well established procedure modified by using more concentrated (10% w/v) aqueous naoh, and by leaving samples in naoh for 2-4 weeks and in chloral hydrate for 3 days, all at room temperature. a single dye, chlorazol black e (1 g/100 ml absolute ethanol), is used to stain for 3-6 min. samples are mounted with the lower epidermis upward. sieve tubes in favorable material can be seen in minor veins and v ...	1986	3750353
secretory glycoproteins of major vestibular glands (bartholin's glands) in female calves. studies with usual histochemical methods and with lectin-horseradish peroxidase conjugates in normal animals and in animals implanted with anabolic drugs.	the nature of mucins synthetized by the major vestibular glands of normal female calves and animals treated by anabolic drugs was investigated by usual histochemical methods and by lectin conjugate methods: canavalia ensiformis (con a), limulus polyphemus (lpa), lotus tetragonolobus (lta), arachis hypogaea (pna), ricinus communis (rca i), glycine max (sba) and triticum vulgaris (wga). two main secretory cell types, i.e. acinar and tubular cells, could be distinguished. the former produced sialom ...	1986	3751491
ultrastructural localization of glycinin and beta-conglycinin in glycine max (soybean) cv. maple arrow by the immunogold method.	beta-conglycinin (7 s globulin) and glycinin (11 s globulin) are the major reserve proteins of soybean. they were localized by the protein a immunogold method in thin sections of glycine max (soybean) cv. maple arrow. in cotyledons, both globulins were simultaneously present in all protein bodies. statistical analysis of marking intensities indicated no correlation between globulin concentration and size of protein bodies. the immunogold method failed to detect either globulin in the embryonic a ...	1986	3759504
a comparison of lectin binding in rat and human peripheral nerve.	eleven different fluorescein- or peroxidase-conjugated lectins with different sugar-binding affinities were employed to analyze and compare glycoconjugates of rat and human peripheral nerves at the light microscopic level. a majority of lectins showed a distinct binding pattern in different structures of the nerve. lectin binding was similar but not identical in rat and human nerves. limulus polyhemus agglutinin did not stain any structures in rat or human nerves. in both species, all other lect ...	1986	3772078
redox reactions of tonoplast and plasma membranes isolated from soybean hypocotyls by free-flow electrophoresis.	redox reactions were studied in more than 90% pure tonoplast and plasma membranes isolated by free-flow electrophoresis from soybean (glycine max) hypocotyls. both types of membrane contained a b-type cytochrome (alpha max = 561 nm) and a noncovalently bound flavin, two possible components of a transmembrane electron-transport chain. isolated tonoplast and plasma membranes reduced ferricyanide, indophenol and various iron complexes with nadh or nadph as electron donors. the redox activity was in ...	1986	3778888
molecular cloning and expression of rhizobium fredii usda 193 nodulation genes: extension of host range for nodulation.	dna hybridization with the cloned nodulation region of rhizobium meliloti as a probe revealed dna homology with four hindiii fragments, 12.5, 6.8, 5.2, and 0.3 kilobases (kb) in size, of the symbiotic plasmid prjausda193. both hybridization and complementation studies suggest that the common nodulation genes nodabc and nodd of r. fredii usda 193 are present on the 5.2-kb hindiii and 2.8-kb ecori fragments, respectively, of the sym plasmid. both fragments together could confer nodulation ability ...	1986	3782034
genetics and breeding of soybeans lacking the kunitz trypsin inhibitor.	the major trypsin inhibitor present in soybean [glycine max (l.) merrill] seed is the kunitz trypsin inhibitor or soybean trypsin inhibitor a2 (sbti-a2). four forms of sbti-a2 have been identified in the u.s. soybean germplasm collection. three of the forms designated ti(a), ti(b), and ti(c) are electrophoretically distinguishable from one another by their different rf values of 0.79, 0.75, and 0.83, respectively. the three forms are inherited as codominant alleles in a multiple allelic series a ...	1986	3799281
preparation of unheated soy protein isolates with low trypsin inhibitor content.	the nutritional quality of soy protein products is affected by the processing conditions employed in their manufacture. heat treatment during processing serves to inactivate the inhibitors of trypsin and chymotrypsin, enzymes which play a key role in the digestion of protein in animals. in the processing of defatted soy flour to prepare a soy protein isolate, generally no heat treatment is used. instead, a high percentage of the trypsin inhibitors (ti) is physically removed in the whey fraction. ...	1986	3799283
relevance of multiple soybean trypsin inhibitor forms to nutritional quality.	trypsin inhibitors contribute to the antinutritional component of raw soybean meal by inhibiting vertebrate pancreatic serine proteinases in the small intestine, resulting in a range of deleterious physiological effects in the animal. the variation in the nutritional quality of soybean cultivars stems partly from wide-ranging levels of trypsin inhibitor, and from varying proportions of trypsin inhibitors of two classes--the kunitz and the bowman-birk inhibitor classes. the latter class is better ...	1986	3799285
photoreactive, active derivatives of trypsin and chymotrypsin inhibitors from soybeans and chickpeas.	the photoreactive arylsufenyl chloride 2-nitro-4-azidophenylsulfenyl chloride (2,4-naps-cl) has been used for the selective modification of tryptophan in kunitz's soybean trypsin inhibitor (sti). the ultraviolet absorption spectrum and amino acid analysis of 2,4-naps-sti indicated that only one of the two tryptophans, 93 or 117, present in sti was modified. amino acid analysis of the two separated cnbr-cleavage products of 2,4-naps-sti showed that only tryptophan 93 underwent modification. 2,4-n ...	1986	3799289
immunological evidence for gap junction polypeptide in plant cells.	a whole cell homogenate prepared from soybean (glycine max (l.) merr. cv. mandarin) root cells (sb-1 cell line) was electrophoresed on a sodium dodecyl sulfate-polyacrylamide gel and transferred to nitrocellulose paper. the nitrocellulose was probed with a monospecific antibody capable of recognizing the mr 27,000 polypeptide of rat liver gap junctions; this antibody was prepared from immune serum raised against gap junctions purified from v79 cells (chinese lung fibroblasts). the immunoblots af ...	1987	3805027
soybean hydrophobic protein. isolation, partial characterization and the complete primary structure.	a 9000-mr protein isolated from a 60% ethanolic extract of soybean (glycine max) seeds has been characterized and fully sequenced. the protein consists of 80 amino acid residues with four disulfide bonds. it contains a large number of hydrophobic residues and lacks methionine, phenylalanine, tryptophan, lysine and histidine residues. the protein readily crystallizes from water but is quite soluble in aqueous organic solvents like 95% 1-propanol. it aggregates to form large molecules (above 80 kd ...	1987	3830151
[selection of parameters for thermal treatment of soybeans by enzyme inactivation].	the effects of thermal treatment, dry heat and steam on the physiologically active substances: urease and trypsin inhibitors of soybean products, were evaluated by means of urease activity and trypsin inhibitor activity. the parameters time and temperature, moisture and particle size were considered. from these analyses it can be concluded that the best conditions to obtain optimum soybean products were 25% of initial moisture content, exposed to steam (97 degrees c) during four to eight minutes ...	1985	3834874
cooking procedures for direct consumption of whole soybeans.	the purpose of this research was to determine adequate conditions for the preparation of soybeans at home level. in accordance with the results, soaking the soybeans for eight hours in a 0.25% bicarbonate solution, gives the appropriate hydration to the soybeans, which can then be easily cooked. boiling them at atmospheric pressure during 20 minutes was required to destroy 80% of the soybeans antitrypsin activity, and a high per was also obtained. nevertheless, the soybeans texture was not satis ...	1985	3836609
elicitor-induced phytoalexin synthesis in soybean cells: changes in the activity of chalcone synthase mrna and the total population of translatable mrna.	rapid changes in the mrna activity encoding chalcone synthase, a central enzyme involved in isoflavonoid phytoalexin synthesis, were induced in cultured cells of soybean (glycine max) after treatment with a glucan elicitor from the cell walls of the fungus, phytophthora megasperma f. sp. glycinea, a soybean pathogen. two-dimensional gel electrophoresis of the in vitro- and in vivo-synthesized chalcone synthase showed that it consisted of a group of proteins of similar molecular weights of about ...	1985	3855251
the bowman-birk inhibitor. trypsin- and chymotrypsin-inhibitor from soybeans.	four decades of studies on the isolation, characterization, properties, structure, function and possible uses of the bowman-birk trypsin- and chymotrypsin-inhibitor from soybeans are reviewed. starting from bowman's acetone insoluble factor, designated ai, aa and sbtiaa, the bowman-birk inhibitor (bbi) was found to be a protein molecule consisting of a chain of 71 amino acids cross linked by 7 disulfide bonds, with a tendency to self-associate. bbi possesses two independent sites of inhibition, ...	1985	3886572
semi-synthetic preparation of 1-o-[1'-14c]hexadecyl-2-acetyl-sn-glycero-3-phosphocholine (platelet activating factor) using plant cell cultures.	incubation of photomixotrophic cell suspension cultures of rape (brassica napus) and heterotrophic cell suspension cultures of soya (glycine max) with 1-o-[1'-14c]hexadecyl-sn-glycerol or rac-1-o-[1'-14c]hexadecylglycerol leads in high yield (up to 78%) to labeled 1-o-hexadecyl-2-acyl-sn-glycero-3-phosphocholines. alkaline hydrolysis of the choline glycerophospholipids yields pure 1-o-[1'-14c]hexadecyl-sn-glycero-3-phosphocholine. 1-o-[1'-14c]hexadecyl-2-acetyl-sn-glycero-3-phosphocholine (plate ...	1985	3891896