Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| uptake of human pharmaceuticals by plants grown under hydroponic conditions. | cabbage (brassica rapa var. pekinensis) and wisconsin fast plants (brassica rapa) were chosen for a proof of concept study to determine the potential uptake and accumulation of human pharmaceuticals by plants. these plants were grown hydroponically under high-pressure sodium lamps in one of two groups including a control and test group exposed to pharmaceuticals. the control plants were irrigated with a recirculating hoagland's nutrient solution while the test plants were irrigated with a hoagla ... | 2010 | 20096438 |
| identification of differential gene expression in brassica rapa nectaries through expressed sequence tag analysis. | nectaries are the floral organs responsible for the synthesis and secretion of nectar. despite their central roles in pollination biology, very little is understood about the molecular mechanisms underlying nectar production. this project was undertaken to identify genes potentially involved in mediating nectary form and function in brassica rapa. | 2010 | 20098697 |
| s-glycoprotein-like protein regulates defense responses in nicotiana plants against ralstonia solanacearum. | rsrga4 (for ralstonia solanacearum-responsive gene a4) encodes a polypeptide similar to s-locus glycoprotein (sgp) from brassica rapa and sgp-like proteins from ipomoea trifida and medicago truncatula. therefore, we designated rsrga4 as ntsglp (for nicotiana tabacum sgp-like protein) and nbsglp (its nicotiana benthamiana ortholog). nbsglp is expressed in root, leaf, petal, gynoecium, and stamen. expression of nbsglp was strongly induced by inoculation with an avirulent strain of r. solanacearum ... | 2010 | 20118275 |
| addition of restriction fragment length polymorphism markers to the genetic linkage map of brassica rapa l. (syn. campestris). | genetic linkage analysis of 151 restriction fragment length polymorphism (rflp) loci, that included eight new loci, detected by the six probes in the present study, and four trait loci including seed colour, leaf pubescence, resistance to white rust caused by albugo candida race-2 (ac-2) and race-7 (ac-7) employing the mapmaker/exp 3.0 programme led to the development of 10 linkage groups (lgs) spanning over 44.4 centimorgan (cm) to 130.4 cm containing 9 to 22 loci and two short lgs with two or ... | 2010 | 20158162 |
| regulation of tissue-specific expression of spatula, a bhlh gene involved in carpel development, seedling germination, and lateral organ growth in arabidopsis. | spatula is a bhlh transcription factor that promotes growth of tissues arising from the carpel margins, including the septum and transmitting tract. it is also involved in repressing germination of newly harvested seeds, and in inhibiting cotyledon, leaf, and petal expansion. using a reporter gene construct, its expression profile was fully defined. consistent with its known functions, spt was expressed in developing carpel margin tissues, and in the hypocotyls and cotyledons of germinating seed ... | 2010 | 20176890 |
| an insertional mutagenesis system for analyzing the chinese cabbage genome using agrobacterium t-dna. | in this study, we applied insertional mutagenesis using agrobacterium transfer dna to functionally characterize the gene of brassica rapa l. ssp. pekinensis. the specific objectives were to: (i) develop and apply a gene tagging system using plasmid rescue and inverse pcr, (ii) select and analyze mutant lines, and (iii) analyze the phenotypic characteristics of mutants. a total of 3,400 insertional mutant lines were obtained from the chinese cabbage cultivar, 'seoul', using optimized condition. p ... | 2010 | 20195907 |
| predicted no effect concentration (pnec) for triclosan to terrestrial species (invertebrates and plants). | triclosan (tcs) is an antimicrobial agent which is used as a broad-spectrum bacteriostatic and found in personal care products, and due to this it is spread in the environment. aquatic toxicity studies have been reported, but little data is available for its toxicity to terrestrial organisms. the main purpose of this study was to assess the toxicity of tcs in the terrestrial environment, using a battery of soil species, belonging to different taxonomic levels. the species included the invertebra ... | 2010 | 20227762 |
| brflc2 (flowering locus c) as a candidate gene for a vernalization response qtl in brassica rapa. | flowering time is an important agronomic trait, and wide variation exists among brassica rapa. in arabidopsis, flowering locus c (flc) plays an important role in modulating flowering time and the response to vernalization. brassica rapa contains several paralogues of flc at syntenic regions. brflc2 maps under a major flowering time and vernalization response quantitative trait locus (qtl) at the top of a02. here the effects of vernalization on flowering time in a double haploid (dh) population a ... | 2010 | 20231331 |
| epigenetic chromatin modifications in brassica genomes. | epigenetic modifications such as histone and dna methylation are highly conserved among eukaryotes, although the nuclear patterns of these modifications vary between different species. brassica species represent a very attractive model for analysis of epigenetic changes because of their differences in genome size, ploidy level, and the organization of heterochromatin blocks. brassica rapa and b. oleracea are diploid species, and b. napus is an allotetraploid species that arose from the hybridiza ... | 2010 | 20237597 |
| effect of atmosphere composition on the quality of ready-to-use broccoli raab (brassica rapa l.). | many leafy brassicas are widely used for ready-to-use salads. broccoli raab (brassica rapa l.), also called turnip top, or rapini, is extensively cultivated in southern italy. the edible portion is made up of the green, immature inflorescences and the stem with its most tender leaves. recently, interest in this crop has increased among european consumers; moreover, a substantial increase in consumption could come from the ready-to-use product. | 2010 | 20355114 |
| effect of organic production and fertilizer variables on the sensory properties of pac choi (brassica rapa var. mei qing choi) and tomato (solanum lycopersicum var. bush celebrity). | the increased popularity of organic production has amplified the need for research that will help in understanding how this production system affects the final quality of vegetables. the effects of organic and conventional production on the sensory characteristics of pac choi (often called bok choy) and tomato were studied. samples were grown in high tunnel and field environments at the kansas state university horticulture research center located in olathe, kansas. highly trained descriptive pan ... | 2010 | 20355138 |
| a rich tilling resource for studying gene function in brassica rapa. | the brassicaceae family includes the model plant arabidopsis thaliana as well as a number of agronomically important species such as oilseed crops (in particular brassica napus, b. juncea and b. rapa) and vegetables (eg. b. rapa and b. oleracea). separated by only 10-20 million years, brassica species and arabidopsis thaliana are closely related, and it is expected that knowledge obtained relating to arabidopsis growth and development can be translated into brassicas for crop improvement. moreov ... | 2010 | 20380715 |
| a comparative study of human health risks via consumption of food crops grown on wastewater irrigated soil (peshawar) and relatively clean water irrigated soil (lower dir). | food crops irrigated with wastewater are mostly contaminated with heavy metals and considered as a main pathway for human exposure. in this study, soil and food crops samples were collected from wastewater irrigated soils, background and relatively less polluted areas. results of the sequential extraction and total metals concentrations in soils indicated that wastewater irrigation has significantly increased (p > or = 0.001) the bioavailable and total metal contents in wastewater irrigated soil ... | 2010 | 20399016 |
| purification and characterization of a peroxidase isozyme from indian turnip roots. | a peroxidase isozyme (tp i) from indian turnip roots ( brassica rapa ) was purified. tp i had a minimum molecular mass of 45 000 da as determined from sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page). a far-uv circular dichroism (cd) spectroscopy study of tp i revealed the presence of 44% alpha-helix, 16% beta-sheet, and 40% random structure. the n-terminal sequence of tp i was found to be gln-phe-val-ile-pro-thr-tyr-ala-trp-gln. pyromellitic dianhydride (pmda)-modified tp i ... | 2010 | 20405843 |
| robust circadian rhythms of gene expression in brassica rapa tissue culture. | circadian clocks provide temporal coordination by synchronizing internal biological processes with daily environmental cycles. to date, study of the plant circadian clock has emphasized arabidopsis (arabidopsis thaliana) as a model, but it is important to determine the extent to which this model applies in other species. accordingly, we have investigated circadian clock function in brassica rapa. in arabidopsis, analysis of gene expression in transgenic plants in which luciferase activity is exp ... | 2010 | 20406912 |
| genetic analysis of interspecific incompatibility in brassica rapa. | in interspecific pollination of brassica rapa stigmas with brassica oleracea pollen grains, pollen tubes cannot penetrate stigma tissues. this trait, called interspecific incompatibility, is similar to self-incompatibility in pollen tube behaviors of rejected pollen grains. since some b. rapa lines have no interspecific incompatibility, genetic analysis of interspecific incompatibility was performed using two f(2) populations. analysis with an f(2) population between an interspecific-incompatibl ... | 2010 | 20414635 |
| [structural and cytochemical aspects of brassica rapa embryogenesis under conditions of clinorotation]. | the results of study of embryo development in b. rapa plants, as well as the rate and the character of nutrient substances accumulation in their cells under slow horizontal clinorotation and laboratory control are presented. significant similarity of the peculiarities of embryo differentiation and character of nutrient substance accumulation in both variants was shown. the cases of different deviations during embryo differentiation, and rate and quantity of reserve nutrient substances in their c ... | 2010 | 20480807 |
| present or past herbivory: a screening of volatiles released from brassica rapa under caterpillar attacks as attractants for the solitary parasitoid, cotesia vestalis. | females of the solitary endoparasitoid cotesia vestalis respond to a blend of volatile organic compounds (vocs) released from plants infested with larvae of their host, the diamondback moth (plutella xylostella), which is an important pest insect of cruciferous plants. we investigated the flight response of female parasitoids to the cruciferous plant brassica rapa, using two-choice tests under laboratory conditions. the parasitoids were more attracted to plants that had been infested for at leas ... | 2010 | 20490899 |
| genetic relationships among turnip (brassica rapa var. rapa) genotypes. | turnip (brassica rapa var. rapa) is one of the main vegetables consumed by people living in eastern anatolia in turkey. in this region, farmers obtain their own seeds for production, which results in considerable morphological variability. we examined the genetic variation and relationships among 11 turnip genotypes sampled from diverse environments of the erzurum region located in eastern anatolia in turkey. thirty-two operon rapd primers were screened; among them, 20 gave reproducible and clea ... | 2010 | 20506086 |
| increase in brao1 gene expression and aldehyde oxidase activity during clubroot development in chinese cabbage (brassica rapa l.). | summary in clubroot disease, gall formation is induced by infection with the obligate biotroph plasmodiophora brassicae due to increased levels of auxins and cytokinins. because aldehyde oxidase (ao) may be involved in auxin biosynthesis in plants, we isolated two ao genes (brao1 and brao2) from chinese cabbage (brassica rapa ssp. pekinensis cv. muso), which are the most similar to aao1 among arabidopsis ao genes, and examined their expressions during clubroot development. the expression of brao ... | 2006 | 20507442 |
| molecular cloning of brassica rapa nitrilases and their expression during clubroot development. | summary three isoforms of nitrilase were cloned from turnip, brassica rapa l., and their expression during clubroot development caused by plasmodiophora brassicae was investigated. the isoforms were designated brnit-t1, brnit-t2 and brnit-t4 based on homology to known nitrilases. brnit-t1 and brnit-t2 have 80% homology to three nitrilases from arabidopsis thaliana (atnit1, atnit2 and atnit3). brnit-t4 showed 90% homology to atnit4. to confirm their enzyme activity, the recombinant proteins were ... | 2007 | 20507526 |
| characterization of seedling proteomes and development of markers to distinguish the brassica a and c genomes. | the diploid species brassica rapa (genome aa) and b. oleracea (genome cc) were compared by full-scale proteome analyses of seedling. a total of 28.2% of the proteins was common to both species, indicating the existence of a basal or ubiquitous proteome. however, a number of discriminating proteins (32.0%) and specific proteins (39.8%) of the brassica a and c genomes, respectively, were identified, which could represent potentially species-specific functions. based on these a or c genome-specific ... | 2010 | 20513634 |
| suppressive effect of yamato-mana (brassica rapa l. oleifera group) constituent 3-butenyl glucosinolate (gluconapin) on postprandial hypertriglyceridemia in mice. | we examined the bioactivity of yamato-mana (brassica rapa l. oleifera group) constituent glucosinolates and found that 3-butenyl glucosinolate (gluconapin) decreased the plasma triglyceride gain induced by corn oil administration to mice. however, phenethyl glucosinolate (gluconasturtiin) had little effect. 2-propenyl glucosinolate (sinigrin) also reduced the plasma triglyceride level, which suggests that alkenyl glucosinolates might be promising agents to prevent postprandial hypertriglyceridem ... | 2010 | 20530888 |
| relation between developmental stage, sensory properties, and volatile content of organically and conventionally grown pac choi (brassica rapa var. mei qing choi). | this study was conducted to identify and quantify the sensory characteristics and chemical profile of organically and conventionally grown pac choi (brassica rapa var. mei qing choi), also called bok choy, at 3 stages of growth (2.5, 4.5, and 6.5 wk). sensory and instrumental data were correlated using partial least squares regression. pac choi was grown in late spring. descriptive sensory analysis was conducted by a highly trained panel and compounds were identified and quantified using a gas c ... | 2010 | 20546419 |
| novel self-compatible lines of brassica rapa l. isolated from the japanese bulk-populations. | self-incompatibility (si) in brassicaceae is sporophytically controlled by a single s-locus with multi allelic variety. the male s determinant, sp11/scr (s-locus protein 11/s-locus cysteine-rich protein), is a small cysteine-rich protein, and the female s determinant, srk (s-locus receptor kinase), functions as a receptor for sp11 at the surface of stigma papilla cells. although a few of the following downstream factors in the sp11-srk signaling cascade have been identified, a comprehensive unde ... | 2010 | 20558895 |
| mapping of quantitative trait loci for high level of self-incompatibility in brassica rapa l. | the level of self-incompatibility (si) is important to the purity of f1 seeds produced using the si system of brassica vegetables. to analyze the genetic basis of the level of si, we generated an f2 population derived from a cross between a turnip inbred line showing a high level of si and a chinese cabbage inbred line showing a low level, and evaluated the level of si under insect pollination in two years. we constructed a detailed linkage map of brassica rapa from the f2 progeny, consisting of ... | 2010 | 20616857 |
| the rapidly evolving centromere-specific histone has stringent functional requirements in arabidopsis thaliana. | centromeres control chromosome inheritance in eukaryotes, yet their dna structure and primary sequence are hypervariable. most animals and plants have megabases of tandem repeats at their centromeres, unlike yeast with unique centromere sequences. centromere function requires the centromere-specific histone cenh3 (cenp-a in human), which replaces histone h3 in centromeric nucleosomes. cenh3 evolves rapidly, particularly in its n-terminal tail domain. a portion of the cenh3 histone-fold domain, t ... | 2010 | 20628040 |
| integration of genetic, physical, and cytogenetic maps for brassica rapa chromosome a7. | bacterial artificial chromosome (bac) contigs have been genetically mapped to the 10 linkage groups of brassica rapa by bac end sequences (bes). to integrate the genetic, physical, and cytogenetic maps, fluorescence in situ hybridization (fish) was used to anchor the assembly of bac contigs onto brassica chromosomes using representative bacs. this bac-fish approach can be used to identify chromosome arms on separate mitotic metaphase chromosomes or to map multiple bacs to single long pachytene c ... | 2010 | 20628251 |
| turnip mosaic virus (tumv) is able to use alleles of both eif4e and eif(iso)4e from multiple loci of the diploid brassica rapa. | three copies of eif4e and three copies of eif(iso)4e have been identified and sequenced from a turnip mosaic virus (tumv)-susceptible, inbred, diploid brassica rapa line, r-o-18. one of the copies of eif4e lacked exons 2 and 3 and appeared to be a pseudogene. the two other copies of eif4e and two of the three copies of eif(iso)4e were isolated from a bacterial artificial chromosome library of r-o-18. using an arabidopsis line (col-0::dspm) with a transposon knock-out of the eif(iso)4e gene which ... | 2010 | 20672877 |
| lactococcus fujiensis sp nov., a lactic acid bacterium isolated from vegetable. | three strains of lactic acid bacteria isolated from vegetable of chinese cabbage (brassica rapa l. var. glabra regel) were characterized taxonomically. they were gram-positive, catalase-negative, facultatively anaerobic cocci that did not produce gas from glucose and formed l-lactic acid. their major fatty acids were c18:1omega9cis, c16:0, c14:0 and summed feature 10. morphological, physiological and phylogenetic data indicated that the strains belong to the genus lactococcus. these strains shar ... | 2010 | 20675438 |
| composition analysis and anti-hypoxia activity of polysaccharide from brassica rapa l. | crude water-soluble polysaccharides (brp) were extracted from the root of brassica rapa l. using boiling-water. the polysaccharides were successively purified by chromatography on deae-cellulose and sephadex g-100 column, giving three major polysaccharide fractions termed brp1-1, brp2-1, brp3-1. the gel permeation chromatography (gpc) analysis showed that the average molecular weight (mw) of polysaccharides (brp1-1, brp2-1, brp3-1) were approximately 5.53×10(3) da, 3.35×10(4) da and 3.37×10(4) d ... | 2010 | 20678519 |
| metabolic changes of brassica rapa transformed with a bacterial isochorismate synthase gene. | metabolome analysis by 1-dimensional proton nuclear magnetic resonance (¹h nmr) coupled with multivariate data analysis was carried out in brassica rapa plants transformed with a gene encoding bacterial isochorismate synthase (ics). partial least square-discrimination analysis (pls-da) on selected signals suggested that the resonances that were dominant in the transgenic plants corresponded to a glucosinolate (neoglucobrassicin), phenylpropanoids (sinapoyl malate, feruloyl malate, caffeoyl malat ... | 2010 | 20705362 |
| screening of dried plant seed extracts for adiponectin production activity and tumor necrosis factor-alpha inhibitory activity on 3t3-l1 adipocytes. | to search for dried plant seeds with potent anti-diabetes activity, we conducted a large scale screening for inhibitory activity on tumor necrosis factor-alpha and facilitating activity on adiponectin production in vitro. these activities in 3t3-l1 adipocytes were screened from ethanol extracts of 20 kinds of dried plant seed marketed in japan. komatsuna (brassica rapa var. perviridis), common bean (phaseolus vulgaris l.), qing geng cai (brassica rapa var. chinensis), green soybean (glycine max) ... | 2010 | 20717728 |
| the quantitative-genetic and qtl architecture of trait integration and modularity in brassica rapa across simulated seasonal settings. | within organisms, groups of traits with different functions are frequently modular, such that variation among modules is independent and variation within modules is tightly integrated, or correlated. here, we investigated patterns of trait integration and modularity in brassica rapa in response to three simulated seasonal temperature/photoperiod conditions. the goals of this research were to use trait correlations to understand patterns of trait integration and modularity within and among floral ... | 2011 | 20736971 |
| chalcone glycosides isolated from aerial parts of brassica rapa l. 'hidabeni' suppress antigen-stimulated degranulation in rat basophilic leukemia rbl-2h3 cells. | we isolated three chalcone glycosides along with other glycoside constituents from the aerial parts of brassica rapa l. 'hidabeni' and examined the effects of these compounds on the antigen-stimulated degranulation in rat basophilic leukemia rbl-2h3 cells. treatments with both 4'-o-β-d-glucopyranosyl-4-hydroxy-3'-methoxychalcone (c1) and 4'-o-β-d-glucopyranosyl-3',4-dimethoxychalcone (c2) markedly inhibited antigen (ag)-stimulated degranulation. to gain further insight into the inhibitory mechan ... | 2010 | 20801664 |
| overexpression of the ethylene-responsive factor gene brerf4 from brassica rapa increases tolerance to salt and drought in arabidopsis plants. | ethylene-responsive factors (erfs), within a subgroup of the ap2/erf transcription factor family, are involved in diverse plant reactions to biotic or abiotic stresses. here, we report that overexpression of an erf gene from brassica rapa ssp. pekinensis (brerf4) led to improved tolerance to salt and drought stresses in arabidopsis. it also significantly affected the growth and development of transgenic plants. we detected that salt-induced expressions of a transcriptional repressor gene, aterf4 ... | 2010 | 20803085 |
| dieldrin uptake and translocation in plants growing in hydroponic medium. | it has been known that the cucurbitaceae family takes up a large amount of persistent organic pollutants from soils and that the translocation of those compounds in cucurbits is higher than those in non-cucurbits. to understand the persistent organic pollutant uptake mechanisms of plant species, we compared the dieldrin absorption and transportation potentials of several plants in hydroponic medium. sorghum (sorghum vulgare moench), sunflower (helianthus annuus l.), soybean (glycine max), komats ... | 2010 | 20821429 |
| floral genetic architecture: an examination of qtl architecture underlying floral (co)variation across environments. | genetic correlations are expected to be high among functionally related traits and lower between groups of traits with distinct functions (e.g., reproductive vs. resource-acquisition traits). here, we explore the quantitative-genetic and qtl architecture of floral organ sizes, vegetative traits, and life history in a set of brassica rapa recombinant inbred lines within and across field and greenhouse environments. floral organ lengths were strongly positively correlated within both environments, ... | 2010 | 20837996 |
| cloning, expression and characterization of the putative nuclear transport factor 2 (ntf2) gene from moss conocephalum conicum(l.) dum. | biomacromolecules import into the nucleus is a complex progress which requires the participation of several cytosolic factors, and nuclear transport factor 2 (ntf2) is one of essential components in nuclear trafficking. its main role is to transport rangdp from cytoplasm to nucleus by interacting with fxfg nucleoporin repeats. in the study a putative new gene, designated as ccntf2, was obtained from the moss (conocephalum conicum) cdna library we have constructed. the full-length cdna sequence i ... | 2011 | 20857212 |
| a brassica exon array for whole-transcript gene expression profiling. | affymetrix genechip® arrays are used widely to study transcriptional changes in response to developmental and environmental stimuli. genechip® arrays comprise multiple 25-mer oligonucleotide probes per gene and retain certain advantages over direct sequencing. for plants, there are several public genechip® arrays whose probes are localised primarily in 3' exons. plant whole-transcript (wt) genechip® arrays are not yet publicly available, although wt resolution is needed to study complex crop gen ... | 2010 | 20862292 |
| phytotoxicity assay for seed production using brassica rapa l. | although pesticide drift can affect crop yield adversely, current plant testing protocols emphasize only the potential impacts on vegetative plant growth. the present study was conducted to determine whether a plant species with a short life cycle, such as brassica rapa l. wisconsin fast plants®, can be used to indicate potential effects on seed production of herbicides applied at relatively low levels (e.g., low field application rates [far]). the effects of ≤0.1 × far of aminopyralid, cloransu ... | 2010 | 20872651 |
| sequence and structure of brassica rapa chromosome a3. | the species brassica rapa includes important vegetable and oil crops. it also serves as an excellent model system to study polyploidy-related genome evolution because of its paleohexaploid ancestry and its close evolutionary relationships with arabidopsis thaliana and other brassica species with larger genomes. therefore, its genome sequence will be used to accelerate both basic research on genome evolution and applied research across the cultivated brassica species. | 2010 | 20875114 |
| construction of an integrated genetic linkage map for the a genome of brassica napus using ssr markers derived from sequenced bacs in b. rapa. | the multinational brassica rapa genome sequencing project (brgsp) has developed valuable genomic resources, including bac libraries, bac-end sequences, genetic and physical maps, and seed bac sequences for brassica rapa. an integrated linkage map between the amphidiploid b. napus and diploid b. rapa will facilitate the rapid transfer of these valuable resources from b. rapa to b. napus (oilseed rape, canola). | 2010 | 20969760 |
| overexpression of the brassica napus bnlas gene in arabidopsis affects plant development and increases drought tolerance. | the gras proteins are a family of transcription regulators found in plants and play diverse roles in plant growth and development. to study the biological roles of gras family genes in brassica napus, an arabidopsis las homologous gene, bnlas and its two homologs were cloned from b. napus and its two progenitor species, brassica rapa and brassica oleracea. relatively high levels of bnlas were observed in roots, shoot tips, lateral meristems and flower organs based on the analysis of the transcri ... | 2011 | 20976458 |
| karyotype and identification of all homoeologous chromosomes of allopolyploid brassica napus and its diploid progenitors. | investigating recombination of homoeologous chromosomes in allopolyploid species is central to understanding plant breeding and evolution. however, examining chromosome pairing in the allotetraploid brassica napus has been hampered by the lack of chromosome-specific molecular probes. in this study, we establish the identification of all homoeologous chromosomes of allopolyploid b. napus by using robust molecular cytogenetic karyotypes developed for the progenitor species brassica rapa (a genome) ... | 2011 | 21041557 |
| a novel detection system for the genetically modified canola (brassica rapa) line rt73. | the herbicide-tolerant genetically modified roundup ready canola (brassica napus) line rt73 has been approved worldwide for use in animal feed and human food. however, rt73 brassica rapa lines derived from interspecific crosses with rt73 b. napus have not been approved in japan. here, we report on a novel system using individual kernel analyses for the qualitative detection of rt73 b. rapa in canola grain samples. we developed a duplex real-time polymerase chain reaction (pcr) method to discrimi ... | 2010 | 21049930 |
| analysis of target sequences of ddm1s in brassica rapa by msap. | dna methylation is an important epigenetic modification regulating gene expression and transposon silencing. although epigenetic regulation is involved in some agricultural traits, there has been relatively little research on epigenetic modifications of genes in brassica rapa, which includes many important vegetables. in b. rapa, orthologs of ddm1, a chromatin remodeling factor required for maintenance of dna methylation, have been characterized and dna hypomethylated knock-down plants by rnai ( ... | 2011 | 21072521 |
| response of glucosinolate and flavonoid contents and composition of brassica rapa ssp. chinensis (l.) hanelt to silica formulations used as insecticides. | silica-based substances have increased in popularity in recent years as alternative insecticides in horticultural crop protection. however, no research has been conducted into the influence of silica on plant biochemistry. formulations (fossil shield 90.0s, ae r974, al-06-109, surround) were applied electrostatically on pak choi. plants were harvested on two dates to measure immediate (first) and decelerated (second) influence, as well as recovery following the removal of silica formulations. th ... | 2010 | 21073193 |
| design of a brassica rapa core collection for association mapping studies. | a brassica rapa collection of 239 accessions, based on two core collections representing different morphotypes from different geographical origins, is presented and its use for association mapping is illustrated for flowering time. we analyzed phenotypic variation of leaf and seed pod traits, plant architecture, and flowering time using data collected from three field experiments and evaluated the genetic diversity with a set of ssr markers. the wageningen university and research centre (wur) an ... | 2010 | 21076504 |
| regional association analysis delineates a sequenced chromosome region influencing antinutritive seed meal compounds in oilseed rape. | this study describes the use of regional association analyses to delineate a sequenced region of a brassica napus chromosome with a significant effect on antinutritive seed meal compounds in oilseed rape. a major quantitative trait locus (qtl) influencing seed colour, fibre content, and phenolic compounds was mapped to the same position on b. napus chromosome a9 in biparental mapping populations from two different yellow-seeded × black-seeded b. napus crosses. sequences of markers spanning the q ... | 2010 | 21076507 |
| development of a high density integrated reference genetic linkage map for the multinational brassica rapa genome sequencing project. | we constructed a high-density brassica rapa integrated linkage map by combining a reference genetic map of 78 doubled haploid lines derived from chiifu-401-42 × kenshin (ckdh) and a new map of 190 f2 lines derived from chiifu-401-42 × rapid cycling b. rapa (crf2). the integrated map contains 1017 markers and covers 1262.0 cm of the b. rapa genome, with an average interlocus distance of 1.24 cm. high similarity of marker order and position was observed among the linkage groups of the maps with fe ... | 2010 | 21076509 |
| growth and development of brassica genotypes differing in endogenous gibberellin content. i. leaf and reproductive development. | leaf and reproductive development were compared in 3 rapid cycling brassica rapa genotypes grown for 4 weeks under greenhouse conditions. the dwarf mutant, rosette (ros), is gibberellin (ga)-deficient, while the tall mutant, elongated internode (ein), has enhanced endogenous ga levels. germination was delayed in ros and a selection of a more severe form of ros, named dormant (do), has even more retarded germination and some seeds entirely fail to germinate. seeds of do and ros respond to exogeno ... | 1990 | 21087278 |
| growth and development of brassica genotypes differing in endogenous gibberellin content. ii. gibberellin content, growth analyses and cell size. | three rapid cycling brassica rapa genotypes were grown in greenhouse conditions to investigate the possible relationships between endogenous gibberellin (ga) content and shoot growth. endogenous ga(1) ga(3) and ga(20) were extracted from stem samples harvested at 3 weekly intervals and analyzed by gas chromatography-mass spectrometry with selected ion monitoring, using [(2) h(2) ]-ga(1) and [(2) h(2) ]-ga(20) as quantitative internal standards. during the first 2 weeks, ga levels of the dwarf, r ... | 1990 | 21087279 |
| developing stable progenies of ×brassicoraphanus, an intergeneric allopolyploid between brassica rapa and raphanus sativus, through induced mutation using microspore culture. | induced mutations were used to improve the low seed fertility of an intergeneric allopolyploid, 'baemoochae,' ×brassicoraphanus, synthesized following hybridization between brassica rapa and raphanus sativus. the mutagen n-methyl-n-nitroso-urethane (nmu) was added to microspore cultures. four lines of nine in the mi(2) generation showed very high fertility under controlled pollination. the progeny lines (mi(3)) confirmed this result under open pollination, and excellent uniformity was observed i ... | 2011 | 21113703 |
| three homologous genes encoding sn-glycerol-3-phosphate acyltransferase 4 exhibit different expression patterns and functional divergence in brassica napus. | brassica napus is an allotetraploid (aacc) formed from the fusion of two diploid progenitors, brassica rapa (aa) and brassica oleracea (cc). polyploidy and genome-wide rearrangement during the evolution process have resulted in genes that are present as multiple homologs in the b. napus genome. in this study, three b. napus homologous genes encoding endoplasmic reticulum-bound sn-glycerol-3-phosphate acyltransferase 4 (gpat4) were identified and characterized. although the three gpat4 homologs s ... | 2010 | 21173024 |
| genomics of biotrophic, plant-infecting plasmodiophorids using in vitro dual cultures. | the plasmodiophorids are a phylogenetically distinct group of parasitic protists that infect plants and stramenopiles, causing several important agricultural diseases. because of the obligate intracellular part of their lifecycle, none of the plasmodiophorids has been axenically cultured. further, the molecular biology of the plasmodiophorids is poorly understood because pure cultures are not available from any species. we report on an in-vitro dual culture system of the plasmodiophorids plasmod ... | 2010 | 21183405 |
| the patterns of population differentiation in a brassica rapa core collection. | with the recent advances in high throughput profiling techniques the amount of genetic and phenotypic data available has increased dramatically. although many genetic diversity studies combine morphological and genetic data, metabolite profiling has yet to be integrated into these studies. for our study we selected 168 accessions representing the different morphotypes and geographic origins of brassica rapa. metabolite profiling was performed on all plants of this collection in the youngest expa ... | 2011 | 21193901 |
| plasticity and evolution in drought avoidance and escape in the annual plant brassica rapa. | a key question in ecological genetics is to what extent do plants adapt to changes in climatic conditions, such as drought, through plasticity or evolution. to address this question, seeds of 140 maternal families of brassica rapa were generated from collections made before (1997) and after (2004) a natural drought. these seeds were planted in the glasshouse and grown under low-water and high-water conditions. post-drought lines flowered earlier than pre-drought lines, showing an evolutionary sh ... | 2011 | 21210818 |
| characterization of brassica nigra collections using simple sequence repeat markers reveals distinct groups associated with geographical location, and frequent mislabelling of species identity. | genetic diversity of 180 brassica nigra (l.) kochgenotypes from 60 different accessions was evaluated using 15 simple sequence repeat markers with known locations on the brassica a, b, and c genomes. two lines each from brassica juncea (l.) czern and brassica carinata braunwere also included as comparator species. a total of 218 high quality alleles were used to generate a genetic distance matrix, and clustering and multidimensional scaling analyses were used to investigate genetic relationships ... | 2011 | 21217806 |
| enhanced glucosinolates in root exudates of brassica rapa ssp. rapa mediated by salicylic acid and methyl jasmonate. | elicitation studies with salicylic acid (sa) and methyl jasmonate (mj) inducing a targeted rhizosecretion of high levels of anticarcinogenic glucosinolates in brassica rapa ssp. rapa plants were conducted. elicitor applications not only led to an accumulation of individual indole glucosinolates and the aromatic 2-phenylethyl glucosinolate in the turnip organs but also in turnip root exudates. this indicates an extended systemic response, which comprises the phyllosphere with all aboveground plan ... | 2011 | 21265537 |
| excision and episomal replication of cauliflower mosaic virus integrated into a plant genome. | transgenic arabidopsis (arabidopsis thaliana) plants containing a monomeric copy of the cauliflower mosaic virus (camv) genome exhibited the generation of infectious, episomally replicating virus. the circular viral genome had been split within the nonessential gene ii for integration into the arabidopsis genome by agrobacterium tumefaciens-mediated transformation. transgenic plants were assessed for episomal infections at flowering, seed set, and/or senescence. the infections were confirmed by ... | 2011 | 21278309 |
| impact of interspecific hybridization between crops and weedy relatives on the evolution of flowering time in weedy phenotypes. | like conventional crops, some gm cultivars may readily hybridize with their wild or weedy relatives. the progressive introgression of transgenes into wild or weedy populations thus appears inevitable, and we are now faced with the challenge of determining the possible evolutionary effects of these transgenes. the aim of this study was to gain insight into the impact of interspecific hybridization between transgenic plants and weedy relatives on the evolution of the weedy phenotype. | 2011 | 21304909 |
| integration of linkage maps for the amphidiploid brassica napus and comparative mapping with arabidopsis and brassica rapa. | the large number of genetic linkage maps representing brassica chromosomes constitute a potential platform for studying crop traits and genome evolution within brassicaceae. however, the alignment of existing maps remains a major challenge. the integration of these genetic maps will enhance genetic resolution, and provide a means to navigate between sequence-tagged loci, and with contiguous genome sequences as these become available. | 2011 | 21306613 |
| construction of random sheared fosmid library from chinese cabbage and its use for brassica rapa genome sequencing project. | as a part of the multinational genome sequencing project of brassica rapa, linkage group r9 and r3 were sequenced using a bacterial artificial chromosome (bac) by bac strategy. the current physical contigs are expected to cover approximately 90% euchromatins of both chromosomes. as the project progresses, bac selection for sequence extension becomes more limited because bac libraries are restriction enzyme-specific. to support the project, a random sheared fosmid library was constructed. the lib ... | 2011 | 21338952 |
| effect of genotype and environmental conditions on health-promoting compounds in brassica rapa. | it is well-known that a variety of factors (genetic and environmental) affect the ultimate metabolite levels in brassica vegetables, although there is still little information about the role that genetics and environment play on glucosinolates and phenolic levels. total glucosinolates were more abundant in turnip tops (26.02 μmol g(-1) dw) than in turnip greens (17.78 μmol g(-1) dw). on the other hand, total phenolic content was found in higher quantities in turnip greens (43.81 μmol g(-1) dw) t ... | 2011 | 21341668 |
| inactivation of muxabc-opmb transporter system in pseudomonas aeruginosa leads to increased ampicillin and carbenicillin resistance and decreased virulence. | resistance-nodulation-cell division (rnd) pumps play important roles in bacterial resistance to antibiotics. pseudomonas aeruginosa is an important human pathogen which exhibits high level resistance to antibiotics. there are total of 12 rnd pumps present in the p. aeruginosa paol genome. the recently characterized muxabc-opmb system has been shown to play a role in resistance to novobiocin, aztreonam, macrolides, and tetracycline in a multiple knockout mutation. in this study, we examined the e ... | 2011 | 21369987 |
| characterization of cenh3 proteins and centromere-associated dna sequences in diploid and allotetraploid brassica species. | cenh3 is a centromere-specific histone h3 variant and has been used as a marker to identify active centromeres and dna sequences associated with functional centromere/kinetochore complexes. in this study, up to four distinct cenh3 (brcenh3) cdnas were identified in individuals of each of three diploid species of brassica. comparison of the brcenh3 cdnas implied three related gene families: brcenh3-a in brassica rapa (aa), brcenh3-b in b. nigra (bb), and brcenh3-c in b. oleracea (cc). each family ... | 2011 | 21394438 |
| phytochemical changes induced by different nitrogen supply forms and radiation levels in two leafy brassica species. | the effect of three different nitrogen (n) supply forms differing in their ammonium-to-nitrate (nh(4):no(3)) ratio (100% nh(4), 50% nh(4) + 50% no(3), 100% no(3)) under three different levels of daily photosynthetic active radiation (par) (low, 5.0; medium, 6.8; high, 9.0 mol m(-2) day(-1)) on a range of desirable health-promoting phytochemicals in brassica rapa subsp. nipposinica var. chinoleifera and brassica juncea was determined. the 100% nh(4) supply under medium par levels led to the highe ... | 2011 | 21395334 |
| genes encoding the biotin carboxylase subunit of acetyl-coa carboxylase from brassica napus and parental species: cloning, expression patterns, and evolution. | comparative genomics is a useful tool to investigate gene and genome evolution. biotin carboxylase (bc), an important subunit of heteromeric acetyl-coa carboxylase (accase) that is a rate-limiting enzyme in fatty acid biosynthesis in dicots, catalyzes atp, biotin carboxyl carrier protein, and co2 to form carboxybiotin carboxyl carrier protein. in this study, we cloned four genes encoding bc from brassica napus l. (namely bnac.bc.a, bnac.bc.b, bnaa.bc.a, and bnaa.bc.b), and two were cloned from e ... | 2011 | 21423283 |
| arvelexin from brassica rapa suppresses nf-+¦b-regulated pro-inflammatory gene expression by inhibiting activation of i+¦b kinase. | background and purpose brassica rapa species constitute one of the major sources of food. in the present study, we investigated the anti-inflammatory effects and the underlying molecular mechanism of arvelexin, isolated from b. rapa, on lipopolysaccharide (lps)-stimulated raw264.7 macrophages and on a model of septic shock induced by lps. experimental approach the expression of inducible nitric oxide synthase (inos) and cox-2, tnf-+¦, il-6 and il-1+¦ were determined by western blot and/or rt-pcr ... | 2011 | 21434881 |
| glyphosate-drift but not herbivory alters the rate of transgene flow from single and stacked trait transgenic canola (brassica napus) to nontransgenic b. napus and b. rapa. | • transgenic plants can offer agricultural benefits, but the escape of transgenes is an environmental concern. in this study we tested the hypothesis that glyphosate drift and herbivory selective pressures can change the rate of transgene flow between the crop brassica napus (canola), and weedy species and contribute to the potential for increased transgene escape risk and persistence outside of cultivation. • we constructed plant communities containing single transgenic b. napus genotypes expre ... | 2011 | 21443650 |
| differences in volatile profiles of turnip plants subjected to single and dual herbivory above- and belowground. | plants attacked by herbivorous insects emit volatile organic compounds that are used by natural enemies to locate their host or prey. the composition of the blend is often complex and specific. it may vary qualitatively and quantitatively according to plant and herbivore species, thus providing specific information for carnivorous arthropods. most studies have focused on simple interactions that involve one species per trophic level, and typically have investigated the aboveground parts of plant ... | 2011 | 21448706 |
| genetic architecture of the circadian clock and flowering time in brassica rapa. | the circadian clock serves to coordinate physiology and behavior with the diurnal cycles derived from the daily rotation of the earth. in plants, circadian rhythms contribute to growth and yield and, hence, to both agricultural productivity and evolutionary fitness. arabidopsis thaliana has served as a tractable model species in which to dissect clock mechanism and function, but it now becomes important to define the extent to which the arabidopsis model can be extrapolated to other species, inc ... | 2011 | 21505830 |
| isolation and characterization of a heme oxygenase-1 gene from chinese cabbage. | heme oxygenase-1 (ho1) is a heme-catabolizing enzyme induced by a variety of stress conditions. this article described the cloning and characterization of brho1 gene which codes for a putative ho1 from chinese cabbage (brassica rapa subsp. pekinensis). brho1 consists of three exons and encodes a protein precursor of 32.3 kd with a putative n-terminal plastid transit peptide. the amino acid sequence of brho1 was 84% similar to arabidopsis counterpart hy1. the three-dimensional structure of brho1 ... | 2011 | 21505948 |
| combined effects of soil moisture and carbaryl to earthworms and plants: simulation of flood and drought scenarios. | studying tolerance limits in organisms exposed to climatic variations is key to understanding effects on behaviour and physiology. the presence of pollutants may influence these tolerance limits, by altering the toxicity or bioavailability of the chemical. in this work, the plant species brassica rapa and triticum aestivum and the earthworm eisenia andrei were exposed to different levels of soil moisture and carbaryl, as natural and chemical stressors, respectively. both stress factors were test ... | 2011 | 21514022 |
| identification of expressed genes during infection of chinese cabbage (brassica rapa subsp. pekinensis) by plasmodiophora brassicae. | abstract. plasmodiophora brassicae is an obligate, biotrophic pathogen causing the club-root disease of crucifers. despite its importance as a plant pathogen, little is known about p. brassicae at the molecular level as most of its life cycle takes place inside the plant host, and axenic culturing is impossible. discovery of genes expressed during infection and gene organization are the first steps toward a better understanding of the pathogen-host interaction. here, suppression subtractive hybr ... | 2011 | 21518080 |
| regulatory hotspots are associated with plant gene expression under varying soil phosphorus supply in brassica rapa. | gene expression is a quantitative trait that can be mapped genetically in structured populations to identify expression quantitative trait loci (eqtl). genes and regulatory networks underlying complex traits can subsequently be inferred. using a recently released genome sequence, we have defined cis- and trans-eqtl and their environmental response to low phosphorus (p) availability within a complex plant genome and found hotspots of trans-eqtl within the genome. interval mapping, using p supply ... | 2011 | 21527424 |
| [in-situ measurement on volatilization loss of ammonia in the vegetable field and its influencing factors]. | in order to obtain ammonia volatilization flux and volatilization loss rate in the vegetable field and investigate their relationship with environmental factors, an on-line monitoring system was used to measure the ammonia volatilization in the vegetable (brassica rapa l. and lettuce) field after urea application during january to september, 2009. the system included a wind tunnel system, a gas collector and an online analyzer system with ion chromatography. the time resolution of measurement wa ... | 2011 | 21528553 |
| polymorphic minisatellites in the mitochondrial dnas of oryza and brassica. | polymorphic analyses of angiosperm mitochondrial dna are rare in comparison with chloroplast dna, because few target sequences in angiosperm mitochondrial dna are known. minisatellites, a tandem array of repeated sequences with a repeat unit of 10 to ~100 bp, are popular target sequences of animal mitochondria, but beta vulgaris is the only known angiosperm species for which such an analysis has been conducted. from this lack of information, it was uncertain as to whether polymorphic minisatelli ... | 2011 | 21562713 |
| a sequence-based genetic linkage map as a reference for brassica rapa pseudochromosome assembly. | brassica rapa is an economically important crop and a model plant for studies concerning polyploidization and the evolution of extreme morphology. the multinational b. rapa genome sequencing project (brgsp) was launched in 2003. in 2008, next generation sequencing technology was used to sequence the b. rapa genome. several maps concerning b. rapa pseudochromosome assembly have been published but their coverage of the genome is incomplete, anchoring approximately 73.6% of the scaffolds on to chro ... | 2011 | 21569561 |
| an undergraduate laboratory exercise to study the effect of darkness on plant gene expression using dna microarray. | dna microarrays are microscopic arrays on a solid surface, typically a glass slide, on which dna oligonucleotides are deposited or synthesized in a high-density matrix with a predetermined spatial order. several types of dna microarrays have been developed and used for various biological studies. here, we developed an undergraduate laboratory exercise using an arabidopsis dna microarray to study the gene expression of brassica rapa, wisconsin fast plant. genes involved in senescence, cell wall l ... | 2007 | 21591140 |
| integration of solexa sequences on an ultradense genetic map in brassica rapa l. | sequence related amplified polymorphism (srap) is commonly used to construct high density genetic maps, map genes and qtl of important agronomic traits in crops and perform genetic diversity analysis without knowing sequence information. to combine next generation sequencing technology with srap, illumina's solexa sequencing was used to sequence tagged srap pcr products. | 2011 | 21595929 |
| comparative methods for association studies: a case study on metabolite variation in a brassica rapa core collection. | association mapping is a statistical approach combining phenotypic traits and genetic diversity in natural populations with the goal of correlating the variation present at phenotypic and allelic levels. it is essential to separate the true effect of genetic variation from other confounding factors, such as adaptation to different uses and geographical locations. the rapid availability of large datasets makes it necessary to explore statistical methods that can be computationally less intensive ... | 2011 | 21602927 |
| combinations of a host resistance gene and the ci gene of turnip mosaic virus differentially regulate symptom expression in brassica rapa cultivars. | in the pathosystem of brassica rapa and turnip mosaic virus (tumv), the type of symptoms expressed by susceptible plants are determined by the gene combinations between the host cultivar and virus strain. in this study, we found that the resistance reaction and symptoms such as systemic lethal necrosis, leaf malformation and mosaic were differentially determined, depending on the combinations of the genotypes for a host locus or two closely linked host loci and the viral ci gene. systemic necros ... | 2011 | 21625976 |
| gravity control of growth form in brassica rapa and arabidopsis thaliana (brassicaceae): consequences for secondary metabolism. | how gravity influences the growth form and flavor components of plants is of interest to the space program because plants could be used for food and life support during prolonged missions away from the planet, where that constant feature of earth's environment does not prevail. we used plant growth hardware from prior experiments on the space shuttle to grow brassica rapa and arabidopsis thaliana plants during 16-d or 11-d hypergravity treatments on large-diameter centrifuge rotors. both species ... | 2009 | 21628221 |
| a novel class of heat-responsive small rnas derived from the chloroplast genome of chinese cabbage (brassica rapa). | non-coding small rnas play critical roles in various cellular processes in a wide spectrum of eukaryotic organisms. their responses to abiotic stress have become a popular topic of economic and scientific importance in biological research. several studies in recent years have reported a small number of non-coding small rnas that map to chloroplast genomes. however, it remains uncertain whether small rnas are generated from chloroplast genome and how they respond to environmental stress, such as ... | 2011 | 21639890 |
| three brassica rapa metallothionein genes are differentially regulated under various stress conditions. | the expression profiles of three brassica rapa metallothionein genes (brmt 1-3) were determined in 7-day-old seedlings exposed to various exogenous factors including plant hormones, heavy metals and abiotic stresses. brmt1, brmt2, and brmt3 were representatives of mt gene type 1, type 2, and type 3, respectively, according to their cysteine alignment. brmt2 showed a relatively higher basal expression level compared to brmt1 and brmt3 under normal conditions. the brmt1 transcript was markedly inc ... | 2012 | 21643753 |
| identification of potential micrornas and their targets in brassica rapa l. | micrornas (mirnas) are recently discovered, noncoding, small regulatory rna molecules that negatively regulate gene expression. although many mirnas are identified and validated in many plant species, they remain largely unknown in brassica rapa (aa 2n =, 20). b. rapa is an important brassica crop with wide genetic and morphological diversity resulting in several subspecies that are largely grown for vegetables, oilseeds, and fodder crop production. in this study, we identified 186 mirnas belong ... | 2011 | 21647586 |
| identification of functional brfad2-1 gene encoding microsomal delta-12 fatty acid desaturase from brassica rapa and development of brassica napus containing high oleic acid contents. | microsomal delta-12 fatty acid desaturase (fad2) functions in the first committed step of the biosynthesis of polyunsaturated fatty acids via the desaturation of oleic acid to linoleic acid. in this study, two fad2 genes were identified through genome-wide analysis of brassica rapa. one brfad2-1 gene harbors functional sequence information, but another brfad2-2 gene has mutations that generated a premature stop codon, rendering it nonfunctional. from a database of 120,000 b. rapa expressed seque ... | 2011 | 21647637 |
| genetic variation in flowering time induces phenological assortative mating: quantitative genetic methods applied to brassica rapa. | it has been argued from first principles that plants mate assortatively by flowering time. however, there have been very few studies of phenological assortative mating, perhaps because current methods to infer paternal phenotype are difficult to apply to natural populations. two methods are presented to estimate the phenotypic correlation between mates-the quantitative genetic metric for assortative mating-for phenological traits. the first method uses individual flowering schedules to estimate ... | 2004 | 21653438 |
| evolutionary ecology of plant-microbe interactions: soil microbial structure alters selection on plant traits. | • below-ground microbial communities influence plant diversity, plant productivity, and plant community composition. given these strong ecological effects, are interactions with below-ground microbes also important for understanding natural selection on plant traits? • here, we manipulated below-ground microbial communities and the soil moisture environment on replicated populations of brassica rapa to examine how microbial community structure influences selection on plant traits and mediates pl ... | 2011 | 21658184 |
| allometry and development in herbaceous plants: functional responses of meristem allocation to light and nutrient availability. | we examined the relationship between meristem allocation and plant size for four annual plant species: arabidopsis thaliana, arenaria serphyllifolia, brassica rapa, and chaenorrhinum minus. gradients of light and nutrient availability were used to obtain a range of plant sizes for each of these species. relative allocation to reproductive, inactive, and growth meristems were used to measure reproductive effort, apical dominance, and branching intensity, respectively. we measured allocation to ea ... | 2003 | 21659133 |
| cloning and characterization of boron transporters in brassica napus. | six full-length cdna encoding boron transporters (bor) were isolated from brassica napus (aacc) by rapid amplification of cdna ends (race). the phylogenic analysis revealed that the six bors were the orthologues of atbor1, which formed companying with the triplication and allotetra-ploidization process of b. napus, and were divided into three groups in b. napus. each group was comprised of two members, one of which was originated from brassica rapa (aa) and the other from brassica oleracea (cc). ... | 2011 | 21660474 |
| phytochrome photoreceptors mediate plasticity to light quality in flowers of the brassicaceae. | the family of phytochrome photoreceptors mediates stem-elongation responses to ambient ratios of red : far-red light (r : fr). although phytochrome genes are expressed in flowers in addition to vegetative parts, nothing is known about floral plasticity to r : fr or the pleiotropic effects of phytochrome genes on flowers. here, the following floral morphologies were compared: (1) wild-type arabidopsis thaliana and brassica rapa plants experiencing high r : fr characteristic of sunlight vs. low r ... | 2002 | 21669731 |
| de novo genetic variation associated with retrotransposon activation, genomic rearrangements and trait variation in a recombinant inbred line population of brassica napus derived from interspecific hybridization with brassica rapa. | interspecific hybridization is a significant evolutionary force as well as a powerful method for crop breeding. partial substitution of the aa subgenome in brassica napus (a(n) a(n) c(n) c(n) ) with the brassica rapa (a(r) a(r) ) genome by two rounds of interspecific hybridization resulted in a new introgressed type of b. napus (a(r) a(r) c(n) c(n) ). in this study, we construct a population of recombinant inbred lines of the new introgressed type of b. napus. microsatellite, intron-based and re ... | 2011 | 21689170 |
| identification and expression analysis of chitinase genes related to biotic stress resistance in brassica. | brassica is a very important vegetable group because of its contribution to human nutrition and consequent economic benefits. however, biotic stress is a major concern for these crops and molecular biology techniques offer the most efficient of approaches to address this concern. chitinase is an important biotic stress resistance-related gene. we identified three genes designated as brassica chitinase like protein (brclp1), brclp2 and brclp3 from a full-length cdna library of brassica rapa cv. o ... | 2011 | 21720758 |
| evaluation of the joint effect of glyphosate and dimethoate using a small-scale terrestrial ecosystem. | in the present work a small-scale terrestrial ecosystem (stem) containing a soil collected from an agricultural field in central portugal was used to evaluate the effects of the combination of the herbicide glyphosate and the insecticide dimethoate. earthworms (eisenia andrei), isopods (porcellionides pruinosus), turnip seeds (brassica rapa), and bait-lamina strips were placed in the stem. the results showed that the application of the recommended field dose of both pesticides did not cause any ... | 2011 | 21724258 |
| development of full-length cdnas from chinese cabbage (brassica rapa subsp. pekinensis) and identification of marker genes for defence response. | arabidopsis belongs to the brassicaceae family and plays an important role as a model plant for which researchers have developed fine-tuned genome resources. genome sequencing projects have been initiated for other members of the brassicaceae family. among these projects, research on chinese cabbage (brassica rapa subsp. pekinensis) started early because of strong interest in this species. here, we report the development of a library of chinese cabbage full-length cdna clones, the riken brc b. r ... | 2011 | 21745830 |
| the genetic architecture of ecophysiological and circadian traits in brassica rapa. | developmental mechanisms that enable perception of and response to the environment may enhance fitness. ecophysiological traits typically vary depending on local conditions and contribute to resource acquisition and allocation, yet correlations may limit adaptive trait expression. notably, photosynthesis and stomatal conductance vary diurnally, and the circadian clock, which is an internal estimate of time that anticipates diurnal light/dark cycles, may synchronize physiological behaviors with e ... | 2011 | 21750258 |
| bnac.tic40, a plastid inner membrane translocon originating from brassica oleracea, is essential for tapetal function and microspore development in brassica napus. | here, we describe the characteristics of a brassica napus male sterile mutant 7365a with loss of the bnms3 gene, which exhibits abnormal enlargement of the tapetal cells during meiosis. later in development, the absence of the bnms3 gene in the mutant results in a loss of the secretory function of the tapetum, as suggested by abortive callose dissolution and retarded tapetal degradation. the bnac.tic40 gene (equivalent to bnms3) was isolated by a map-based cloning approach and was confirmed by g ... | 2011 | 21756273 |