Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| chloroperoxidase: p-450 type absorption in the absence of sulfhydryl groups. | the oxidation state of the two half-cystine residues in the native ferric form of chloroperoxidase and in the reduced ferrous chloroperoxidase has been examined in order to evaluate the role of sulfhydryl groups as determinants of p-450 type spectra. mössbauer and optical spectroscopy studies indicate that the ferrous forms of p-450cam and chloroperoxidase have very similar or identical heme environments. model studies have suggested that sulfhydryl groups may function as axial ligands for devel ... | 2003 | 170955 |
| cytochemical correlates of structural sexual dimorphism in glandular tissues of the mouse. i. studies of the renal glomerular capsule. | the parietal epithelium of bowman's capsule has been analyzed by enzyme cytochemistry in kidneys of mice (c57bl/6j) from birth to 50 days of age. there is a greater tendency for cells in the central portions of the capsular crescent to be cuboidal in post-pubertal males than in pre-pubertal mice of either sex or in post-pubertal females where they are generally squamous; moreover, these heightened capsular cells have a distinct microvillous border. cytochemical procedures were selected which mig ... | 1975 | 171242 |
| electrical stimulation of the brain. iv. ultrastructural studies. | the ultrastructural response of tissues of the cat's central nervous system to chronic electrical stimulation with various parameters has been evaluated at four and a half days post stimulation. cellular alterations included widespread cytoplasmic vacuolations, architectural derangement and disruption of plasmalemma and other membranes. lipid inclusions and dense crystalline bodies were prominent in astrocyte-like phagocytic cells in the more severe lesions. despite endothelial damage in brain p ... | 1975 | 171783 |
| proton magnetic resonance studies of peroxidases from turnip and horseradish. | proton nmr spectra at 270 mhz have been measured for horseradish peroxidase and turnip peroxidase isoenzymes (p1, p2, p3 and p7) in both their high spin ferric native states and as the low spin ferric cyanide complexes. resonances of amino acids near the heme have been identified and used to investigate variations in the structure of the heme crevice amongst the enzymes. ligand proton resonances have been resolved in spectra of the cyanide complexes of the peroxidases and these provide informati ... | 1975 | 172144 |
| chicken neutrophils: oxidative metabolism in phagocytic cells devoid of myeloperoxidase. | polymorphonuclear leukocytes isolated from chicken peritoneal exudates have been found to catalyze cyanide-insensitive stimulation of respiration and the hexose monophosphate shunt upon exposure to heat-inactivated staphylococcus aureus. however, there was no demonstrable formate oxidation concomitant with phagocytosis in either the presence or absence of exogenous catalase. moreover, chicken polymorphonuclear leukocytes failed to oxidize scopoletin concomitant with phagocytosis in the presence ... | 1975 | 174090 |
| cellular localization of cyclic amp in periodontal tissues during experimental tooth movement in cats. | using an immune-histochemical method, cyclic amp was localized in cells of periodontal tissues in orthodontically-treated cats. sixteen cats were treated for periods ranging from 1 hour to 4 weeks. fresh, frozen, undecalcified 6 mu sections of the tissues were incubated with rabbit anti-cyclic amp antibodies, followed by sequential incubations with sheep anti-rabbit igg, rabbit anti-peroxidase igg and horseradish peroxidase. in the final step, the peroxidase was demonstrated by the diaminobenzid ... | 1976 | 175896 |
| [further purification and immunohistological localization of human placental lactogen in syncytiotrophoblasts (author's transl)]. | in 1936, ehrhadt reported a prolactin-like substance which has been identified as a second protein hormone exists in extracted substance from the placenta. to date various reports have been published, but ther are still unsolved problems concerning the specifity of the substance, and its clinical significance is unclear. the purpose of this project was to purify and investigate the biochemical, immunological properties of hpl, and try to localize hpl secretion immunohistologically. the biologica ... | 1976 | 176070 |
| studies on the chlorinating activity of myeloperoxidase. | two methods were utilized to demonstrate the peroxidation of chloride ion to a free species (hocl or cl2) by myeloperoxidase. the peroxidase caused the volatilization of radioactivity from soultions containing hydrogen peroxide and [36cl]nacl, and catalyzed the formation of hocl when solutions contianing these components were passed through a millipore filter to which the peroxidase was adsorbed. in this flow system, 90 mug of canine myeloperoxidase generated 80 mum hocl in the presence of 200 m ... | 1976 | 176150 |
| use of horseradish peroxidase to study the antagonism of clostridium welchii (cl. perfringens) type d epsilon toxin in mice by the formalinized epsilon prototoxin. | 1976 | 176185 | |
| subcellular localization of b apoprotein of plasma lipoproteins in rat liver. | multispecific antigen-binding fragments (fab) from rabbit antisera against rat very low density lipoproteins (vldl) and fab against rat low density lipoproteins that were monospecific for the b apoprotein were conjugated to horseradish peroxidase. conjugates were incubated with 6-mum frozen sections from fresh and perfusion-fixed livers and with tissue chopper sections (40 mum thick) from perfusion-fixed livers. in the light microscope, specific reaction product was present in all hepatocytes of ... | 1976 | 177430 |
| peroxidase-conjugate chromatography isolation of conjugates prepared with glutaraldehyde or periodate using polyacrylamide-agarose gel. | fractionation abilities of polyacrylamide-agarose gel (ultrogel) and dextran gel (sephadex) column chromatography were compared in isolating horseradish peroxidase conjugates, prepared using two different methods. utrogel aca-44 provides an efficient separation of monomer conjugated and nonconjugated immunoglobulins resulting from the two-step glutaraldehyde procedure, sephadex g-200 does not. both types of columns eluted the polymer conjugates resulting from the periodate procedure in the void ... | 1976 | 177698 |
| modification and regeneration of synaptic connections in cultured leech ganglia. | segmental ganglia of the central nervous system of the leech were maintained in culture medium outside the animal for several weeks in order to study the properties of synapses and regeneration by identified sensory and motor nerve cells. a variety of preparations were used, including single ganglia, chains of ganglia and ganglia connected to the areas of skin and muscle that they normally innervate in the animal. (1) for up to 10 weeks after removal from the animal, resting and action potential ... | 1976 | 181202 |
| ultrastructural basis for alveolar-capillary permeability to protein. | the intravenous injection into mice of small volumes (less than 0.1 ml) of peroxidatic enzymes of molecular weight of 40 000 daltons or greater results in little if any penetration of these probe molecules into endothelial junctions. the injection of cytochrome c (12 000 daltons), on the other hand, results in the localization of this tracer in some but not all endothelial junctions. when horseradish peroxidase (ec 1.11.1.7) is injected in a large volume of saline (0.5 ml), reaction product is p ... | 1976 | 181220 |
| secretion-related uptake of horseradish peroxidase in neurohypophysial axons. | during secretion of the neurohypophysial hormones, oxytocin and vasopressin, secretory granule membrane is added to the plasma membrane of the axon terminals. it is generally assumed that subsequent internalization of this additional membrane occurs by endocytosis. in order to study this process, we have traced the uptake of intravenously injected horseradish peroxidase by neurohypophysial axons in rats and golden hamsters. peroxidase reaction product within the secretory axons was found mainly ... | 1976 | 181385 |
| direct immunoperoxidase staining for regan isoenzyme of alkaline phosphatase in human tumor tissues. | immunoperoxidase staining for regan isoenzyme of alkaline phosphatase was performed on cryostat sections of five human tumor tisssues. with a direct immunoperoxidase staining for the localization of regan isoenzyme at the light and electron microscope levels, sections previously fixed with 0.05 m phosphate-buffered 4% paraformaldehyde were reacted with rabbit antisera to human placenta alkaline phosphatase conjugated to horseradish peroxidase. comparison of conventional histochemistry and immuno ... | 1976 | 182352 |
| early events in polyoma virus infection: attachment, penetration, and nuclear entry. | the plaque-assay technique was used as a tool to determine the optimal conditions for adsorption of polyoma virions to host cells. using these optimal conditions of adsorption, an electron microscopy study of the early events of infection was performed. by electron microscopy and autoradiography, it was demonstrated that both the viral coat proteins and dna arrive simultaneously in the nucleus as early as 15 min postinfection. when horseradish peroxidase-labeled virions, pseudovirions, and capsi ... | 1976 | 183018 |
| peroxidase and fluorescein isothiocyanate as antibody markers. a quantitative comparison of two peroxidase conjugates prepared with glutaraldehyde or periodate anda fluorescein conjugate. | batches of rabbit anti-human immunoglobulin g antibodies were labeled either with horseradish peroxidase, using the two-step glutaraldehyde method or the periodate method, or with fluorescein isothiocyanate (fitc). the peroxidase conjugates were isolated by chromatography using two different gel types. the five types of conjugates thus obtained were standardized to the same amount of rabbit immunoglobulin g. the antibody activity, as estimated by means of single radial immunodiffusion and passiv ... | 1976 | 184204 |
| beneficial effect of catalase treatment on growth of clostridium perfringens. | several common plating media were tested for their ability to support growth of clostridium perfringens after storage of the plates for 1 to 10 days at 4 and 25 degrees c with and without subsequent addition of catalase. liver-veal (lv) agar and brain heart infusion (bhi) agar quickly become incapable of supporting growth after storage without added catalase, whereas shahidi ferguson perfringens (sfp) agar and brewer anaerobic (ba) agar were less affected. plate counts of c. perfringens on untre ... | 1976 | 185958 |
| production of the superoxide radical by horseradish peroxidase. | 1976 | 187464 | |
| horseradish peroxidase. xxv. an electron spin resonance study of the low temperature photochemical reaction of compound i. | 1977 | 189760 | |
| hydrogen peroxide production and killing of staphylococcus aureus by human polymorphonuclear leukocytes. | the effects of bacterial neuraminidase on production of hydrogen peroxide (h2o2) and killing of staphylococcus aureus by human polymorphonuclear leukocytes (pmn) were studied. the concentration of h2o2 was measured by the disappearance of scopoletin fluorescence in the presence of horseradish peroxidase. the results indicated that desialylation of human pmn inhibited the stimulation of h2o2 production during phagocytosis. it also markedly impaired the killing of s. aureus. impaired killing of s. ... | 1977 | 189859 |
| free radical intermediate in the n-demethylation of aminopyrine by horseradish peroxidase-hydrogen peroxide. | 1977 | 190040 | |
| relay cell classes in the lateral geniculate nucleus of the cat and the effects of visual deprivation. | this study presents evidence that the x- and y-cells described physiologically in the a laminae of the cat's dorsal lateral geniculate nucleus (lgn) are two morphologically distinct cell types recognizable in golgi preparations. it is shown firstly that the three cell types seen in golgi preparations of the a laminae (large and medium-sized principal cells and small interneurons-types 1,2 and 3 in the classification of guillery, '66) may be identified in 1-mum epon sections of osmicated material ... | 1977 | 190274 |
| sensitized photooxidation of low spin horseradish peroxidase. | 1977 | 191009 | |
| analysis and computer simulation of aerobic oxidation of reduced nicotinamide adenine dinucleotide catalyzed by horseradish peroxidase. | under suitable experimental conditions the aerobic oxidation of nadh catalyzed by horseradish peroxidase occurred in four characteristic phases: initial burst, induction phase, steady state, and termination. a trace amount of h2o2 present in the nadh solution brought about initial burst in the formation of oxyperoxidase. about 2 mol of oxyperoxidase was formed per mol of h2o2. when a considerable amount of the ferric enzyme still remained, the initial burst was followed by an induction phase. in ... | 1977 | 192280 |
| mechanism of thyroxine-mediated oxidation of reduced nicotinamide adenine dinucleotide in peroxidase-h2o2 system. | the oxidation of reduced nicotinamide adenine dinucleotide (nadh) by the horseradish peroxidase (hrp)-h2o2 system is greatly increased by the addition of thyroxine or related compounds. on the basis of a study of the rate of nadh oxidation in the presence of various concentrations of thyroxine, it is clear that thyroxine acts as a catalyst for nadh oxidation. spectral changes of a hrp-h2o2 complex (compound i) indicate that thyroxine acts as an electron donor to both compounds i and ii. the rate ... | 1977 | 192281 |
| tissue-specific antibodies against human lung and breast carcinoma dehistonized chromatins. | tissue-specific antisera against human lung and breast carcinoma dehistonized chromatins were obtained. the specificity of these antisera was determined by complement fixation. in the presence of antiserum against human lung carcinoma, only chromatins from lung carcinoma fixed complement significantly, whereas chromatins isolated from human breast carcinoma, hela cells, normal lung tissue, breast tissue, or term placenta were negative (i.e., inactive). in a similar assay with the use of antiseru ... | 1977 | 195066 |
| solid-phase enzyme immunoassay for immunoglobulin m antibodies to cytomegalovirus. | a sensitive, solid-phase enzyme immunoassay for the detection of immunoglobulin m antibodies to cytomegalovirus is described. the results of the enzyme immunoassay correlated well with those obtained by an indirect immunofluorescence method. horseradish peroxidase proved to be a more sensitive label than alkaline phosphatase. nonspecific reactions, occurring with commercially available cytomegalovirus antigens, could be avoided by using a nuclear antigen prepared from sonically disrupted nuclei ... | 1977 | 195977 |
| protein a-peroxidase: a valluable tool for the localization of antigens. | protein a of staphylococcus aureus has been conjugated to horseradish peroxidase and used in an indirect immunolabeling technique to visualize membrane and viral antigens. the same protein a-peroxidase conjugate was used with antisera from five different species. using this indirect test, membrane markers for t and b lymphocytes were labeled with a greater specificity than when peroxidase conjugated anti-immunoglobulin was used in the second step. viral antigens on cells infected with measles, v ... | 1977 | 199666 |
| electron-paramagnetic-resonance studies on a photochemically produced species of horseradish peroxidase compound i. | strong electron-paramagnetic-resonance signals in the g = 2.00 region were detected after irradiation of horseradish peroxidase compound i at temperatures of 10 and 100 k. these signals establish the presence of new free-radical species in the peroxidase system. the new species are interpreted in terms of a haem-photosensitized oxidation of the protein's peptide groups close to the compound i radical site. on warming to room temperature, the radicals decayed irreversibly to a species having a we ... | 2003 | 201247 |
| probing axial ligands in ferric haemoproteins: an esr study of myoglobin and horseradish peroxidase in h217o. | 1977 | 202277 | |
| rat plasma clearance of horseradish peroxidase and yeast invertase is mediated by specific recognition. | 1978 | 202501 | |
| formation and elimination of foreign synapses on adult salamander muscle. | 1. synapses by flexor nerve were induced on denervated extensor muscle in adult salamander forelimbs. excitatory potentials evoked by these ;foreign' synapses were at first small but increased to normal amplitude within several weeks, in the absence of correct nerve reinnervation.2. upon return of the correct nerve the efficacy of foreign synaptic transmission began to decline. the time of initiation of this decline correlated well with the resumption of correct nerve transmission. the suppressi ... | 1987 | 203675 |
| intracellular demonstration of growth hormone in human mammary carcinoma cells. | intracellular growth hormone has been demonstrated in human mammary carcinoma cells by using an unlabeled antibody enzyme technic with horseradish peroxidase and antihorseradish peroxidase complex. intense reaction was seen in both male and female mammary carcinoma cells. positive staining was observed in nuclei and cytoplasm and on cytoplasmic membranes. | 1978 | 204210 |
| the permeability of epidermis lacking normal membrane-coating granules: an ultrastructural tracer study of kyrle-flegel disease. | skin lesions from patients with flegel's disease have been reported to be without membrane-coating granules (odland bodies). biopsies of the hyperkeratinized papules of kyrle-flegel disease were incubated in vitro with the intercellular tracer, horseradish peroxidase, and the extent of penetration of this substance examined in the light and electron microscopes. the peroxidase was present throughout the corium and extended through the intercellular spaces of the epidermis to a level close to the ... | 1993 | 206629 |
| intracellular horseradish peroxidase injection for correlation of light and electron microscopic anatomy with synaptic physiology of cultured mouse spinal cord neurons. | synaptic interactions between spinal cord neurons grown in dissociated cell culture were studied electrophysiologically, and presynaptic cells were subsequently injected by intracellular iontophoresis with horseradish peroxidase (hrp). following histochemical processing, injected cells were filled with dense reaction product which facilitated the light and electron microscopic identification of the individual physiologically typed neurons. this technique applied to neurons in monolayer culture a ... | 1978 | 209872 |
| studies on the mechanism of inhibition of redox enzymes by substituted hydroxamic acids. | substituted primary hydroxamic acids were found to inhibit the catalytic activity of a number of redox enzymes. the inhibition was not related to the nature of the metal-active site of the enzyme nor to the nature of the oxygen-containing substrate. two easily available enzymes, mushroom tyrosinase (monophenol,dihydroyphenylalanine:oxygen oxidoreductase, ec 1.14.18.1) and horseradish peroxidase (donor:hydrogen-peroxide oxidoreductase, ec 1.11.1.7), which were potently inhibited by hydroxamic aci ... | 1990 | 210815 |
| ["functional and anatomical organization of associative" cortical areas 7, 21 and 22 in monkeys. a study of the cortical projections from the pulvinar by means of hrp tracing technique (author's transl)]. | the thalamic projections to the parietal and temporal associative areas (brodmann's areas 7, 21 and 22) have been revealed by means of the retrograde axonal transport of horseradish peroxidase (hrp). the medial pulvinar (pm) was found to send afferents to the three studied areas. labelled neurones of the pm appeared to be grouped in terms of their cortical target; indeed, neurones of origin of projection fibres to areas 7, 21 and 22 were respectively localized in the dorso-lateral, ventro-latera ... | 1978 | 211571 |
| comparison of an enzyme-linked immunosorbent assay for quantitation of rotavirus antibodies with complement fixation in an epidemiological survey. | the development of a micro-scale enzyme-linked immunosorbent assay (elisa) with horseradish peroxidase as the marker enzyme for the detection and measurement of human rotavirus antibodies is described. a semipurified preparation of the serologically related simian agent, sa-11 virus, was used as the antigen. test sera were reacted with antigen-sensitized wells in disposable poly-vinyl microplates. any attached antibody was detected by the addition of peroxidase-labeled anti-species immunoglobuli ... | 1978 | 212451 |
| the chemical properties of out-of-equilibrium states of proteins and the role of these states in protein functioning. | the out-of-equilibrium states of several iron-containing proteins (cytochromes c of different origin, haemoglobin, myoglobin, ferredoxin and other non-haem iron proteins, cytochrome c oxidase, horseradish peroxidase) were recorded after fast changes in the active centre (electron reduction of iron, ligand dissociation). strained states result in which the active centre has already been changed and undergone vibrational relaxation but the main part of protein globule is in the 'old', now out-of-e ... | 1977 | 215393 |
| oxidation of 2-nitropropane by horseradish peroxidase. involvement of hydrogen peroxide and of superoxide in the reaction mechanism. | incubation of aqueous solutions of 2-nitropropane in air causes a slow oxidation reaction that generates h(2)o(2). purified horseradish peroxidase catalyses the oxidation of such preincubated 2-nitropropane solutions according to the equation: [formula: see text] the ph optimum is 4.5 and k(m) for 2-nitropropane is 16mm. other nitroalkanes or nitro-aromatics tested are not oxidized at significant rates by peroxidase. h(2)o(2) or 2,4-dichlorophenol increases the rate of 2-nitropropane oxidation b ... | 1978 | 217346 |
| contacts between receptors and electrophysiologically identified neurones in the retina of the larval tiger salamander. | 1. following the intracellular recording of bipolar and horizontal cell responses, each unit was injected with horseradish peroxidase and a histochemical staining used to identify it at the level of the light and electron microscopes.2. centre-depolarizing bipolar cells made contact with rods and cones at basal and ribbon junctions, the latter being fewer. centre-hyperpolarizing bipolar cells made the same types of contacts with the receptors, but ribbon junctions predominated.3. it appears, the ... | 1978 | 217992 |
| anatomical relationships between the ventral mesencephalic tegmentum--a 10 region and the locus coeruleus as demonstrated by anterograde and retrograde tracing techniques. | we have searched for anatomical connections between the ventral mesencephalic tegmentum (vmt), including the dopaminergic cell group a10 and the locus coeruleus (lc) region. tritiated leucine (120--220 nl) and horseradish peroxidase delivered by electrophoresis were injected in the vmt-a10 region. we have demonstrated, on the one hand bilateral projections from the vmt-a10 region to lc, and on the other hand a possible contralateral projection from lc to vmt-a10 region. these relationships, repo ... | 1979 | 220380 |
| contribution of the locus coeruleus to the adrenergic innervation of the rat spinal cord: a biochemical study. | the possible existence and magnitude of a noradrenergic innervation from the locus coeruleus (lc) to the spinal cord was investigated in the rat with various techniques. horseradish peroxidase, injected into the lumbar spinal cord produced heavy labelling of presumably noradrenaline (na)-containing neurons in the ventral region of the lc, while cells in the dorsal region of the lc were only lightly labelled. the effects of electrothermic destruction and electrical stimulation of the lc on levels ... | 1979 | 220381 |
| chemical and enzymatic intermediates in the peroxidation of o-dianisidine by horseradish peroxidase. 1. spectral properties of the products of dianisidine oxidation. | 1979 | 221005 | |
| [horseradish peroxidase-labeled cells as sources of the spinoreticular and spinothalamic systems of the brain]. | the cells of spinoreticular and spinothalamic fibrous systems of the cat brain were studied by the method of axone transmission of horse-radish peroxidase (hp). a dense accumulation of hp-labeled neurons establishing direct relations with the reticular formation and thalamus was seen in the upper segments of the spinal cord. in the lower segments these zones were confined to the medial part of the ventral horn and the intermediate zone of the gray matter. the neurons established direct connectio ... | 1979 | 222379 |
| horseradish peroxidase compound i: evidence for spin coupling between the heme iron and a 'free' radical. | 1979 | 223878 | |
| two different and compatible intraneuronal labels for ultrastructural study of synaptically related cells. | to examine the structural interactions between synaptically connected neurons in aplysia, we have developed a method for simultaneously labeling two identified cell with different and compatible intraneuronal marking agents (horseradish peroxidase and [3h]n-acetyl-d-galactosamine) visible in both the light and electron microscopes. combining these two agents within a single cell yields a third label. | 1979 | 226210 |
| some aspects of the organization of the lateral geniculate nucleus in galago senegalensis revealed by using horseradish peroxidase to label relay neurons. | following injection of horseradish peroxidase into area 17 of the prosimian galago senegalensis, columns of labeled neurons are seen in the dorsal lateral geniculate nucleus extending through all cell layers. individual counts of the number of labeled and unlabeled neurons reveal that from 91 to 98% of all neurons within these densely labeled columns are labeled. these results indicate that most of the neurons within the lgn of the bushbaby project to striate cortex. average diameter measurement ... | 1979 | 226217 |
| raphe projections to the locus coeruleus in the rat. | afferent projections to the locus coeruleus from the various raphe nuclei, particularly of the midbrain (nuclei raphe dorsalis and medianus) and pons (nuclei raphe pontis and magnus), have been studied in the rat by retrograde transport methods using horseradish peroxidase (hrp). the locus coeruleus, in both its dorsomedial and ventrolateral divisions, and in its various anterior-posterior components, were injected with 0.05 microliters of horseradish peroxidase following which various structure ... | 1979 | 226233 |
| heme-environment of horseradish peroxidase as observed by oxygen-17 superhyperfine interaction in epr. | 1979 | 227384 | |
| tegmentoreticular projections with special reference to the muscular atonia during paradoxical sleep in the cat: an hrp study. | using the retrograde tracer technique with horseradish peroxidase (hrp), attempts were made to determine the cells of origin and the descending pathway of the tegmentoreticular projections in order to give an anatomical substrate for the physiological phenomenon of the postural atonia observed during paradoxical sleep (ps) in the cat. the hrp was injected into various parts of the pontomedullary reticular formation (rf) including the caudal raphe nuclei, nucleus (n.) reticularis gigantocellulari ... | 1979 | 227527 |
| periodate or glutaraldehyde for preparing peroxidase conjugates? | conjugates of horseradish peroxidase with antibodies (anti-human igg (h + l)) or their fab' fragments were prepared according to the newest modification of the periodate (p-) method or the two-step glutaraldehyde (g-) method. the conjugates were analysed by gel chromatography and subsequently tested in three different applications. for tissue immunohistochemistry on sections of lupus erythematosus skin, g-conjugates were preferred to polymeric p-conjugates. in elisa for detection of human antibo ... | 1979 | 227964 |
| resonance raman study on yeast cytochrome c peroxidase. effect of coordination and axial ligands. | resonance raman spectra are reported for native ferric cytochrome c peroxidase, its cyanide and fluoride compounds, those of the ferrous enzyme and its cyanide and carbonyl compounds, and the spectrum of the hydrogen peroxide compound, compound i. band frequencies of ferric horseradish peroxidase isoenzyme c2 and its derivatives are also given. comparison of the frequencies of the bands around 1400, 1500, 1560-1580, and 1610-1640 cm-1 with those of other hemoproteins and heme model compounds sho ... | 1979 | 228743 |
| acth-like immunoreactivity in two electronically coupled giant neurons in the pond snail lymnaea stagnalis. | two giant neurons (diameter 130 micron) were identified immunocytochemically by means of the unlabeled antibody enzyme technique with anti-acth 1-39 and 1-24 in the pond snail lymnaea stagnalis. the cells are located in the visceral and the right parietal ganglion, respectively. they contain moderately electron dense elementary granules (diameter 150-160 nm). by means of the intracellular horseradish peroxidase injection technique it was shown that the cells send fibres into the neuropiles of va ... | 1979 | 229956 |
| enzyme-linked protein a: an enzyme-linked immunosorbent assay reagent for detection of human immunoglobulin g and virus-specific antibody. | a general-purpose reagent capable of reacting with immunoglobulin g in a modified enzyme-linked immunosorbent assay technique was prepared by using protein a coupled with horseradish peroxidase. the reagent detected low levels (0.003 to 1.0 microgram/ml) of human immunoglobulin g and was also applied in an enzyme-linked immunosorbent assay for titration of antibody to human cytomegalovirus. the antibody titers to human cytomegalovirus determined by enzyme-linked immunosorbent assay and by comple ... | 1979 | 231048 |
| horseradish peroxidase. xxxiv. oxidation of compound ii to i by periodate and inorganic anion radicals. | the one-electron oxidation of horseradish peroxidase compound ii to compound i by sodium periodate was observed. the bimolecular rate constant for the naio4--compound ii interaction is equal to 9.5 +/- 1 x 10(-3) m-1s-1 at room temperature. irradiation, using ultraviolet light, of the solution containing compound ii and persulfate in the presence of bicarbonate, chloride, or bromide, leads ot the fast accumulation of compound i due to the oxidative action of so4, co3, cl2, and br2 anion radicals ... | 1979 | 232001 |
| [purification of horseradish peroxidase conjugated igg by affinity chromatography. comparison of glutaraldehyde and periodate conjugation]. | by affinity chromatography of the coupling mixture on concanavalin-a-sepharose unlabeled igg is completely removed. hrp conjugated igg is then separated from free hrp by gelfiltration. glutaraldehyde conjugation yielded 20 to 68% unlabeled igg, depending on the duration of glutaraldehyde addition, and periodate conjugation yielded 10 to 28% unlabeled igg. by this method fractions of conjugates with 3fold spec. act. were obtained by the glutaraldehydemethod and with 1.6fold spec. act. by periodat ... | 1979 | 232345 |
| nuclear magnetic resonance evidence for the absence of iron coordinated water in horseradish peroxidase. | 1975 | 234224 | |
| the interaction of 5-hydroxytryptamine and related hydroxyindoles with horseradish and mammalian peroxidase systems. | 1975 | 234231 | |
| california encephalitis virus development in mosquitoes as revealed by transmission studies, immunoperoxidase staining, and electron microscopy. | isolates of the snowshoe hare subtype of california encephalitis (ce) virus from yukon mosquitoes during 1972 and 1973 were transmitted by bites of aedes aegypti mosquitoes after 4 to 5 weeks of extrinsic incubation at 55 degrees f after intrathoracic injection, and the 1973 strain was transmitted after mosquitoes were fed virus and held for 3 to 4 weeks at 75 degrees f. antigen of a 1971 isolate of ce virus (marsh lake 23) was detected in salivary glands of infected mosquitoes by the immunopero ... | 1975 | 235355 |
| kinetics of the oxidation of p-aminobenzoic acid catalyzed by horseradish peroxidase compounds i and ii. | the kinetics of p-aminobenzoic acid oxidation catalyzed by horseradish peroxidase compounds i and ii was investigated intensively as a function of ph at 25 degrees in aqueous solutions of ionic strength 0.11. all of the rate data were collected from single turnover experiments involving reactions of a single enzyme compound. in reactions of both compounds, deviations from first order behavior with respect to the enzyme were observed at high ph values which were explained in terms of a free radic ... | 1977 | 235530 |
| a kinetic study of the reaction of horseradish peroxidase with hydrogen peroxide. | a kinetic study has been carried out over the ph range of 2.63-9.37 for the reaction of horseradish peroxidase with hydrogen peroxide to form compound i of th;e enzyme. analysis of the results, indicates that there are two kinetic influencing, ionizable groups on the enzyme with pka values of 3.2 and 3.9. protonation of these groups results in a decrease in the rate of reaction of the enzyme with h2o2. a previous study of the kinetics of cyanide binding to horseradish peroxidase (ellis, w.d. & d ... | 1975 | 237615 |
| effects of 2,4-substituents of deuteropheme upon redox potentials of horseradish peroxidases. | 1975 | 239639 | |
| endocytosis and multivesicular body formation in rabbit luteal cells during pseudopregnancy. | horseradish peroxidase (hrp) was injected directly into the corpora lutea of rabbits on different days of pseudopregnancy. young luteal cells have also been incubated "in vitro" in a medium containing horseradish peroxidase. in the "in vivo" experiments the 5 to 9 days old luteal cells take up far more horseradish peroxidase than the older ones (14-20 days). different types of endocytic vacuoles, mvb's and also some db's are already peroxidase positive shortly (15-20 min) after injection of the ... | 1975 | 240507 |
| fractionation of horseradish peroxidase by preparative isoelectric focusing, gel chromatography and ion-exchange chromatography. | horseradish peroxidase has been fractionated by preparative isoelectric focusing in a density gradient and in a layer of granulated gel using ph-3-10 and narrow-ph-range carrier ampholytes at different total enzyme loads. the resolution of peroxidase isoenzymes in preparative-layer isoelectric focusing was comparable to that obtained by analytical thin-layer isoelectric focusing. isoelectrically homogeneous isoenzymes could be isolated with good recovery in a single fractionation step. despite t ... | 1975 | 240683 |
| preparation and properties of matrix-supported horseradish peroxidase. | a new method of enzyme immobilization has been described using poly(4-methacryloxybenzoic acid) as the carrier. activation of the polymer, prior to enzyme attachment, was achieved with n-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline. the enzyme coupling step proceeded through nucleophilic attack by the protein on a mixed carbonic anhydride. the degree of polymer activation was determined by analysis for quinoline, a by-product of the reaction. the polymer-enzyme complex was compared to the enzyme ... | 1975 | 241471 |
| the anatomical organization of septo-hippocampal projections. | since the time of elliot smith (1910) it has been recognized that the septal complex occupies a pivotal position within the mammalian telencephalon, being strategically placed between the hippocampal formation on the one hand and the basal forebrain and diencephalon on the other. however, it is only in the last few years that the detailed interrelationships between the different nuclear groups within the septum and the various subfields of the hippocampus have been studied. we have recently re-e ... | 1968 | 252443 |
| trigeminal nerve and temporomandibular joint of the cat: a horseradish peroxidase study. | 1979 | 262237 | |
| specific projection of displaced retinal ganglion cells upon the accessory optic system in the pigeon (columbia livia). | in the pigeon, the nucleus of the basal optic root, a component of the accessory optic system, projects directly upon the vestibulo-cerebellum. this nucleus receives a prominent projection composed of large-diameter retinal axons, known as the basal optic root. the cells of origin of this tract were identified using horseradish peroxidase (donor:hydrogen-peroxide oxidoreductase, ec 1.11.1.7) as a retrograde marker. injections of horseradish peroxidase confined primarily to the basal optic root n ... | 1993 | 266216 |
| identification of synaptic acetylcholine receptor sites in retina with peroxidase-labeled alpha-bungarotoxin. | an alpha-bungarotoxin-horseradish peroxidase conjugate, which binds specificity to nicotinic acetylcholine receptors, was synthesized. this conjugate was bound by 5-7% of the synapses in the inner plexiform layer of the chicken retina. bipolar ribbon synapses as well as amacrine synapses bound the conjugate. | 1977 | 269390 |
| conjugation of poly-l-lysine to albumin and horseradish peroxidase: a novel method of enhancing the cellular uptake of proteins. | the carbodiimide-catalyzed conjugation of a 6700 molecular weight fragment of poly-l-lysine to radiolabeled human serum albumin or to horseradish peroxidase enhances the membrane transport of each protein into cultured mouse fibroblasts approximately 11- and 200-fold, respectively. at least 50% of the peroxidase activity remained after conjugation. trypsinization and carbamylation of the two conjugates demonstrates that the enhancement of their cellular uptake is related to their poly-l-lysine c ... | 1978 | 273916 |
| ultrastructural observations of horseradish peroxidase in synovial lining cells of the rats' temporomandibular joint. | 1977 | 274462 | |
| evidence for receptor-mediated binding of glycoproteins, glycoconjugates, and lysosomal glycosidases by alveolar macrophages. | alveolar macrophages have been shown to bind glycoproteins and synthetic glycoconjugates (neoglycorpoteins) that have mannose, n-acetylglucosamine, or glucose in the exposed, nonreducing position. galactose-terminal glycoproteins are not bound. binding of radiolabeled ligands to cells is nearly completely impaired by the presence of an excess of yeast mannan. binding is temperature sensitive and proceeds optimally at ph 7.0. prior treatment of the cells with trypsin severely decreases their capa ... | 1978 | 274729 |
| endocytosis of cholera toxin into neuronal gerl. | cholera toxin linked covalently by glutaraldehyde to horseradish peroxidase was incubated with cultured chicken sympathetic neurons at 4 degrees. cells were washed and brought to 37 degrees to permit endocytosis of bound toxin on plasma membranes. massive internalization of the ligand into vesicles and cisterns of the golgi--endoplasmic reticulum--lysosome (gerl) system was demonstrated by the cytochemical reaction for the enzyme. surface binding and subsequent endocytosis of the cholera toxin-- ... | 1978 | 275852 |
| effect of photocoagulation on the barrier function of the pigment epithelium. ii. a study by electron microscopy. | the healing of retinal photocoagulation burns and their permeability to colloidal carbon and horseradish peroxidase was studied by electron microscopy. recent burns showed disruption of intercellular junctions in the pigment epithelium. by 2 days there was infiltration of the burn by proliferating pigment epithelial cells, and by 7 days the burn was composed of a mass of interconnected cells of pigment epithelial and müller cell origin. fresh photocoagulation burns result in complete disruption ... | 1977 | 281795 |
| modification of the blood-brain barrier: increased concentration and fate of enzymes entering the brain. | the blood-brain barrier of rats was opened reversibly by infusing a hyperosmotic solution of arabinose into the external carotid artery. permeability was increased maximally in the first 15 min and remained slightly elevated at 1 hr. osmotic barrier opening significantly increased brain uptake of intravenously injected alpha-mannosidase (alpha-d-mannoside mannohydrolase, ec 3.2.1.2.4) (derived from human placenta) and horseradish peroxidase (donor:hydrogen-peroxide oxidoreductase, ec 1.11.1.7). ... | 1979 | 284363 |
| the effect of serotonin on the blood-brain barrier to proteins. | since increased concentration of serotonin (5-ht) has been demonstrated in areas of the brain exposed to ischemia and lesions, and since the elevation might be responsible for the enhanced permeability to proteins across cerebral vessels, studies were carried out to elucidate the effect of the amine, perfused through the cerebral ventricular system, on the transport of horseradish peroxidase (hrp) from blood to brain. the amounts of 5-ht were large (50--800 microgram per mouse). the permeability ... | 1978 | 290745 |
| localization of horseradish peroxidase-alpha-bungarotoxin binding in crustacean axonal membrane vesicles and intact axons. | a conjugate of alpha-bungarotoxin with horseradish peroxidase was used to visualize alpha-bungarotoxin binding sites at the fine structural level in isolated axonal membrane vesicles from lobster walking leg nerve. these plasma membrane vesicles have previously been shown to exhibit saturable binding of [3h]nicotine and [3h]acetylcholine. binding of the toxin was identified in the axon plasma membrane and could be blocked by pretreatment with excess free alpha-bungaratoxin or d-tubocurarine. bin ... | 1979 | 291022 |
| retrograde axonal transport of horseradish peroxidase from cornea to trigeminal ganglion. | horseradish peroxidase (hrp) was dripped on the scarified left cornea of adult mice. twenty-four hours later the animals were fixed by vascular perfusion and frozen sections cut from both trigeminal ganglia. after incubation for peroxidase activity labelled nerve cells were restricted to the medial ophthalmic part of the ganglion ipsilateral to hrp administration. if the scarification was omitted no neuronal labelling was observed. this labelling of the neurons is most probably the result from a ... | 1977 | 300978 |
| topographic projections between the nucleus isthmi and the tectum of the frog rana pipiens. | the connections between the nucleus isthmi and the tectum in the frog have been determined by several anatomical techniques: iontophoresis of horseradish peroxidase into the tectum, iontophoresis of 3h-porline into the nucleus isthmi and the tectum, and fink-heimer degeneration staining after lesions of the nucleus isthmi. the results show that the nucleus isthmi projects bilaterally to the tectal lobes. the ipsilateral isthmio-tectal fibers are distributed in the superficial layers of the tectu ... | 1978 | 305927 |
| [study of the connections of the thalamus in rana temporaria frogs by the peroxidase method]. | in frogs, on the base of the axonal transport of horseradish peroxidase, the connections ascending to the primordial hippocamp were shown from the ipsi- and contralateral pyriform pallium, contralateral primordial hippocamp, ispilateral amygdala and nucleus accumbens, as well as from rostral parts of the postero-central thalamic nucleus (bilaterally). the ascending connections from the dorsal column nuclei were followed with reliability till the rostral parts of the contralateral mesencephalic t ... | 1995 | 306728 |
| effect of prostaglandin e2 on the permeability of the iris vessels to horseradish peroxidase in the rabbit. | prostaglandin e2 (0.5 microgram in 10 microliter 10% ethanol) was introduced into the anterior chambers of rabbit eyes. using the horseradish peroxidase method, it was shown under the electron microscope that the endothelial barrier of the iris vessels broke down. the peroxidase penetrated as far as the basis of the posterior epithelial cells, however, without entering their lateral intercellular spaces. the question of whether the effect of prostaglandins on the barrier was a direct effect or a ... | 1978 | 309739 |
| extravascular transfer within the anuran pars intermedia. | to study the transport of protein from the blood into and throughout the sparsely vascularized pars intermedia of anurans, the electrondense tracer, horseradish peroxidase (hrp) was injected into the vascular system of adult frogs. a strong reaction product was localized in small vesicles in the cytoplasm of that portion of the stellate cells immediately beneath the vascular spaces. also, within two minutes after an injection of hrp, which was given during a period of one minute, the reaction pr ... | 1978 | 309794 |
| afferent and efferent components of the facial nerve in the bullfrog (rana catesbeiana). | the afferent and efferent components of the facial nerve were traced within the brain stem of rana catesbeiana, using three different neuroanatomical techniques. primary afferent fibers could be traced to the spinal tract of trigeminal nerve and to fasciculus solitarius as far caudally as the first or second spinal segment, using silver degeneration methods. cobalt filling of of the entire nerve showed the same distribution of afferent fibers, as well as the filling of the cells within the mesen ... | 1979 | 310889 |
| efferent connections from the brain to the frontal organ in rana temporaria demonstrated by labeling with horseradish peroxidase. | after injection of horseradish peroxidase into the frontal organ of rana temporaria, labeled perikarya were found in the medial part of the amygdala, the preoptic area, the nucleus rotundus, the pretectal area, and the lateral parts of the midbrain central griseum. | 1979 | 311252 |
| some telencephalic connections in the frog, rana pipiens. | some afferent, efferent and intrinsic connections of the telencephalon of rana pipiens were studied using a horseradish peroxidase method. afferents to the telencephalon from thalamic and brain stem cell groups were demonstrated. these findings, taken together with the results of previous studies, indicate that separate thalamic cell groups project visual, auditory and somatosensory information onto the striatum. a separate thalamic cell group projects to the medial telencephalic wall and probab ... | 1979 | 311788 |
| intracellular localization of markers within injected or cut frog muscle fibers. | many experimental procedures require drastic alterations of muscle fibers, such as cutting the fiber or injecting molecular probes through microelectrodes. we report the ultrastructure of similarly altered muscle fibers and the intracellular distribution of injected horseradish peroxidase (hrp). cut fibers appear structurally normal at distances greater than 500 microm from the cut end, however, the structure deteriorates nearer to the cut. hrp diffuses longitudinally about 2,000 micrometer from ... | 1979 | 313713 |
| distribution of anti-hrp antibodies in the central nervous system of immunized rats after disruption of the blood brain barrier. | lewis rats were immunized with horseradish peroxidase (hrp) injected intradermally in the hind foot pads; anti-hrp antibodies in the cerebrum were detected with a cytochemical reaction for hrp in sections incubated with the antigen (hrp), or after intravenous injection of hrp a few minutes before sacrifice. in two groups of animals the blood brain barrier was disrupted by a heat lesion or by a traumatic lesion (insertion of a canula into the lateral ventricle). in animals with a heat or with a t ... | 1979 | 313978 |
| localization of rhodopsin in isolated, osmotically intact rod outer segment discs. | localization of rhodopsin and its position in the membrane has been the subject of numerous studies. most recently, immunocytochemical techniques have been employed to localize the opsin component of the molecule in in situ rod outer segments. due to the problems inherent in localization procedures (penetration and mechanical interference) we have utilized isolated, osmotically intact rod outer segment discs in this study. specific antibodies to chromatographically pure rhodopsin were prepared a ... | 1979 | 314460 |
| rapid stimulation of pinocytosis in human carcinoma cells a-431 by epidermal growth factor. | horseradish peroxidase (hrp) uptake was used to measure fluid-phase pinocytosis in monolayers of human epithelioid carcinoma cells (a-431). histochemistry confirmed that cell-associated hrp was restricted to intracellular vesicles. biochemical methods showed that hrp uptake in control cultures was directly proportional to the duration of exposure. the addition of low concentrations of epidermal growth factor (egf) to the incubation media produced a 10-fold increase in the initial rate of pinocyt ... | 1979 | 315944 |
| retrograde transport of horseradish peroxidase in sensory and adrenergic neurons following injection into the anterior eye chamber. | horseradish peroxidase (hrp) was injected into the anterior eye chamber of rats and mice and frozen sections from both superior cervical and trigeminal ganglia were incubated to demonstrate neurons accumulating the tracer by retrograde axonal transport. labelled cells were observed only in ganglia ipsilateral to the hrp injection. within the trigeminal ganglion, peroxidase-containing neurons were restricted to the medial ophthalmic area, whereas labelled cells in the superior cervical ganglion w ... | 1979 | 317295 |
| caudate afferents from the retrorubral nucleus and other midbrain areas in the cat. | in order to clarify which midbrain areas project to the caudate nucleus (cd), horseradish peroxidase (hrp) was deposited in the cd of 13 cats. midbrain sections were reacted with diaminobenzidine and sometimes with cobaltous chloride to intensify the reaction, and then examined for retrograde labelling. afferents to the cd were found arising from the following ipsilateral midbrain areas: pars compacta, pars reticulata, and pars lateralis of the substantia nigra (sn), ventral tegmental area of ts ... | 1978 | 318187 |
| the functional capacity of guinea pig megakaryocytes. ii. the uptake of particles and macromolecules and the effect of rabbit antiguinea pig platelet antiserum. | the capacity of megakaryocytes to take up particles and macromolecules was tested by exposing them to homologous erythrocytes, latex particles, micrococcus lysodeikticus, and horseradish peroxidase. uptake of particles 1.1 to 7 mum. in size was extensive under the experimental conditions employed. after uptake of each of the three particles the addition of tannic acid and calcium to the fixative produced electron-dense deposits within the vacuole surrounding the particle; therefore, the vacuole ... | 1977 | 320386 |
| an ultrastructure study on antibody production of the lymph nodes of rats with special reference to the role of germinal centers. | antibody-containing cells in lymph nodes, expecially in germinal centers, and in thoracic duct of rats sensitized by injection of horseradish peroxidase were studied with the immunoperoxidase method. in the primary response the large germinal center cells began to produce antibody on the 9th day and predominated thereafter in germinal centers ,added by intercellular antibody deposition in its late stage. the "ordinary" medium-sized germinal center cells were a minor component. in the secondary r ... | 1977 | 322442 |
| intimal healing. the pattern of reendothelialization and intimal thickening. | studies were undertaken to investigate further the basis of intimal proliferation and the identity of the surface lining cell in rabbit aortas subjected to extensive deendothelialization. endothelial cells were selectively removed by passage of an inflated balloon catheter through the arterial lumen. the healing response was evaluated at intervals up to 36 weeks by several techniques: 1) permeability to evans blue, 2) reappearance of endothelial cells as indicated by the specific marker, adsorbe ... | 1977 | 322503 |
| solid-phase enzyme immunoassay for hepatitis b surface antigen. | a solid-phase enzyme immunoassay is described for measuring hepatitis b surface antigen in human serum or plasma. immunologically purified antibody labeled with horseradish peroxidase was used as the indicator. in the assay system, antibody-coated controlled-pore glass is used as a solid support and there are three sequential incubations, totaling 2 h, at room temperature. results for serially diluted positive and reference sera compare favorably to radioimmunoassay in sensitivity and specifici ... | 1977 | 322892 |
| ultrastructural immunocytochemical localisation of lysozyme in human bronchial glands. | an immunocytochemical unlabelled antibody method using rabbit antihuman lysozyme, antirabbit immunoglobulin, and soluble rabbit antihorseradish peroxidase/horseradish peroxidase complexes was used to study the fine structural distribution of lysozyme in human bronchial glands. none was identified in mucous cells but there was heavy staining of the serous cell granules. the serous cell granules were not stained uniformly, suggesting the presence of other secretory products but lysozyme secretion ... | 1977 | 325682 |
| electron microscopic observations of cell coat of childhood leukemic cells using concanavalin a-horseradish peroxidase method. | 1977 | 327742 | |
| [retinal projections in the lamprey, lampetra fluviatilis (horseradish perioxidase transport method in the optic nerve)]. | after the injection of horseradish peroxidase into the eye of the lamprey l. fluviatilis, the anterograde transport of the enzyme was found in the optic tract itself reaching the optic tectum and via single fibers--the geniculate nucleus. the labeled fibers were also found in the axial optic tract terminating in the geniculate nucleus and in the nucleus of the posterior commissurae. retrograde transport and labeling of the tracer were observed in cells of the dorsal and central tegmentum. | 2006 | 331759 |