Publications

influence of preceding crop on wireworm (coleoptera: elateridae) abundance in the coastal plain of north carolina.	three studies were conducted to determine the effect of preceding crop on wireworm (coleoptera: elateridae) abundance in the coastal plain of north carolina. in all three studies, samples of wireworm populations were taken from the soil by using oat, avena sativa l., baits. treatments were defined by the previous year's crop and were chosen to reflect common crop rotations in the region. across all three studies, eight wireworm species were recovered from the baits: conoderus amplicollis (gyllen ...	2010	21309229
transcriptome characterization and high throughput ssrs and snps discovery in cucurbita pepo (cucurbitaceae).	cucurbita pepo belongs to the cucurbitaceae family. the "zucchini" types rank among the highest-valued vegetables worldwide, and other c. pepo and related cucurbita spp., are food staples and rich sources of fat and vitamins. a broad range of genomic tools are today available for other cucurbits that have become models for the study of different metabolic processes. however, these tools are still lacking in the cucurbita genus, thus limiting gene discovery and the process of breeding.	2011	21310031
effects of cucumber mosaic virus infection on vector and non-vector herbivores of squash.	plant chemicals mediating interactions with insect herbivores seem a likely target for manipulation by insectvectored plant pathogens. yet, little is currently known about the chemical ecology of insect-vectored diseases or their effects on the ecology of vector and nonvector insects. we recently reported that a widespread plant pathogen, cucumber mosaic virus (cmv), greatly reduces the quality of host-plants (squash) for aphid vectors, but that aphids are nevertheless attracted to the odors of ...	2010	21331245
[effects of exogenous ca2+ on morphological and photosynthetic characteristics and chlorophyll fluorescent parameters of squash seedlings under high temperature and strong light stress].	taking squash (cucurbita pepo l.) variety alan as test object, this paper studied the effects of exogenous ca2+ on the morphological and photosynthetic characteristics and chlorophyll fluorescent parameters of squash seedlings under the cross-stress of high temperature and strong light. under the stress, applying 5-20 mmol x l(-1) of ca2+ increased the plant height, leaf area, chlorophyll and carotenoid contents, photosynthetic rate (pn), stoma conductance (gs), transpiration rate (tr), maximal ...	2010	21361006
watermelon chlorotic stunt virus (wmcsv): a serious disease threatening watermelon production in jordan.	the incidence of watermelon chlorotic stunt disease and the molecular characterization of the jordanian isolate of watermelon chlorotic stunt virus (wmcsv-[jo]) are described in this study. symptomatic leaf samples obtained from watermelon (citrullus lanatus thunb.), melon (cucumis melo l.), squash (cucurbita pepo), cucumber (cucumis sativus l.), and bottle gourd (lagenaria siceraria) plants were tested for wmcsv-[jo] infection by pcr. the virus could be detected in 8 melon and 87 watermelon sam ...	2011	21399920
influence of plant-earthworm interactions on som chemistry and p,p'-dde bioaccumulation.	laboratory experiments assessed how bioaccumulation of weathered p,p'-dde from soil and humic acid (ha) chemistry are affected by interactions between the plants cucurbita pepo ssp. pepo and ssp. ovifera and the earthworms eisenia fetida, lumbricus terrestris, and apporectodea caliginosa. total organochlorine phytoextraction by ssp. pepo increased at least 25% in the presence of any of the earthworm species (relative to plants grown in isolation). uptake of the compound by ssp. ovifera was unaff ...	2011	21421253
selection of reference genes for gene expression studies in zucchini (cucurbita pepo) using qpcr.	the zucchini (cucurbita pepo) is an important food crop, the transcriptomics of which are a fundamental tool to accelerate the development of new varieties by breeders. however, the suitability of reference genes for data normalization in zucchini has not yet been studied. the aim of this study was to assess the suitability of 13 genes for their potential use as reference genes in quantitative real-time pcr. assays were performed on 34 cdna samples representing plants under different stresses an ...	2011	21476515
populations of predators and parasitoids of bemisia tabaci (hemiptera: aleyrodidae) after the application of eight biorational insecticides in vegetable crops.	the sweetpotato whitefly, bemisia tabaci (gennadius) (hemiptera: aleyrodidae), is an important pest of vegetables and many other crops worldwide. eight biorational insecticides (based on oil, plant derivatives, insect growth regulator and fungus) were evaluated in the field for their influence on populations of six natural enemies of b. tabaci. natural populations of two predators [chrysoperla carnea stephen (neuroptera: chrysopidae) and orius spp. (hemiptera: anthocoridae)] and two genera of pa ...	2011	21480463
photochemical immobilization of cucurbita fruit ascorbate oxidase onto polyethylene disc for determination of ascorbate in serum and foodstuffs.	abstract: an ascorbate oxidase purified from the green fruit of zucchini squash (cucurbita pepo medullosa) was immobilized photochemically on a polyethylene disc with 85% retention of initial activity of free enzyme. the optimum ph (5.5) was unchanged, while k(m) was decreased. the polyethylene discs were employed for determination of ascorbic acid in serum and foodstuffs. the working linear range was 2.8 μm to 16 μm. the mean value of ascorbic acid in serum as measured by the method was 0.16 mg ...	2011	21506664
congener specificity in the accumulation of dioxins and dioxin-like compounds in zucchini plants grown hydroponically.	zucchini cultivars cucurbita pepo subsp. ovifera cv. patty green and subsp. pepo cv. gold rush were cultivated hydroponically in a nutrient solution supplemented with a mixture of dioxins and dioxin-like compounds. patty green and gold rush showed low and high accumulation of these compounds in the aerial parts respectively. in both cultivars, the accumulation of each congener negatively depended on its hydrophobicity. this suggests that desorption and solubilization were partly responsible for ...	2011	21512252
effect of pumpkin root exudates on ex situ polychlorinated biphenyl (pcb) phytoextraction by pumpkin and weed species.	a greenhouse experiment was conducted to determine if cucurbita pepo ssp. pepo (pumpkin) root exudates could increase the uptake of polychlorinated biphenyls (pcbs) into plants. contaminated soil was pre-treated with pumpkin root exudates by first growing pumpkins in the soil. plants (pumpkins and weeds) were grown in the pre-treated (root exudate group) and non-treated (control group) contaminated soils. seeds from five weed species collected from two contaminated sites were germinated in suffi ...	2011	21559906
was the famous astronomer copernicus also a nephrologist.	nicolaus copernicus (1473-1543), world-famous astronomer, born in toruń, was also a warmian canon (senior priest) and a physician to 4 consecutive prince-bishops of warmia and of other warmian canons. what medical conditions preoccupied nicolaus copernicus and whether they included kidney diseases can only be inferred from the extant prescriptions of copernicus, as no record remains of any treatises by copernicus regarding medicine. while no prescription penned by him is dated, several are trace ...	2011	21614777
development and characterization of polymorphic microsatellite markers in momordica charantia (cucurbitaceae).		2010	21616876
occurrence of clostridium perfringens from different cultivated soils.	the occurrence of clostridium perfringens was estimated in 750 samples originated from a variety of soils bearing various bulb crops: brawnica oderacea (vegetable), olea europaea, daucus carota (carote), solanum tuberosum (potato), phaseolus vulgaris (green haricot), beta vulgaris var. rapaceum (beetroot), cucurbita pepo (squash), allium cepa (onion), cucumis sativus (cucumber) and capsicum annum (pepper). all isolated strains were tested for their antimicrobial activities to amoxicillin, penici ...	2011	21621626
antimicrobial nectar inhibits a florally transmitted pathogen of a wild cucurbita pepo (cucurbitaceae).	• premise of the study: floral nectars of many species contain antimicrobial chemicals, but their function in nectar is subject to debate. previously, we have shown that erwinia tracheiphila, the causative agent of bacterial wilt disease in cucurbits, can be transmitted via the floral nectaries. • methods: we used a disk diffusion assay (dda) to determine the antimicrobial effects of nectar from a wild gourd on lawns of escherichia coli and erwinia tracheiphila. we also used e. tracheiphila to i ...	2010	21622472
leaf herbivory increases floral fragrance in male but not female cucurbita pepo subsp. texana (cucurbitaceae) flowers.	mutualisms are key interactions that affect population dynamics and structure communities, but the extent to which mutualists can attract potential partners may depend on community context. many studies have shown that leaf herbivory reduces pollinator visitation and have focused on reduced floral visual display and rewards as potential mechanisms. however, olfactory display plays a critical role in mediating interactions between plants, herbivores, and pollinators. we simulated leaf damage in c ...	2009	21628242
darkness affects differentially the expression of plastid-encoded genes and delays the senescence-induced down-regulation of chloroplast transcription in cotyledons of cucurbita pepo l. (zucchini).	in contrast to differentiated leaves, the regulatory mechanisms of chloroplast gene expression in darkened cotyledons have not been elucidated. although some results have been reported indicating accelerated senescence in arabidopsis upon reillumination, the capacity of cotyledons to recover after dark stress remains unclear. we analysed the effect of two-days dark stress, applied locally or at the whole-plant level, on plastid gene expression in zucchini cotyledons. our results showed that in t ...	2011	21630590
resistance and tolerance to herbivory changes with inbreeding and ontogeny in a wild gourd (cucurbitaceae).	herbivory is a ubiquitous component of terrestrial communities that reduces plant growth and reproduction. consequently, a goal of evolutionary ecology is to identify the causes and consequences of variation in herbivory within plant populations. this three-year study examined the effects of inbreeding on the resistance of wild gourd plants (cucurbita pepo subsp. texana) to herbivory by cucumber beetles and the impact of the timing of herbivory on reproduction. we grew families of inbred and out ...	2008	21632318
inbreeding effects on blossom volatiles in cucurbita pepo subsp. texana (cucurbitaceae).	self-pollination by plants gives rise to inbreeding depression. there is increasing recognition that plant inbreeding can have significant implications for interactions between plants and other organisms, including insects and pathogens. many of these interactions are mediated by plant-derived volatiles, but the effects of inbreeding on volatile production have not previously been investigated. we examined variation in flower volatile production by the wild gourd cucurbita pepo subsp. texana as ...	2006	21642122
a comparison of male and female responses to inbreeding in cucurbita pepo subsp. texana (cucurbitaceae).	accurate estimates of inbreeding depression are necessary in order to predict the evolutionary dynamics of a population, but many studies estimate inbreeding depression based solely on components of female function such as fruit set, seed set, and seed quality. because total fitness is achieved through both male and female functions in hermaphroditic plants, estimates of both male and female fitness are needed to estimate accurately the magnitude of inbreeding depression. seedlings of a wild gou ...	2005	21652390
the absorption and translocation of polychlorinated biphenyl congeners by cucurbita pepo ssp pepo.	the mobility of polychlorinated biphenyl (pcb) congeners within cucurbita pepo ssp pepo cv. howden (pumpkin), a pcb phytoextracting plant, was investigated through a comparison of field-weathered soil, root, shoot, and xylem sap congener profiles. this is the first study to show the presence of pcbs in xylem sap (range: 0.03-0.18 μg·ml(-1)), confirming that pcb translocation throughout the plant occurs via this medium. a comparison of soil (5.2 ± 2.5 μg·g(-1)), root (27.1 ± 2.1 μg·g(-1)), shoot ...	2011	21696136
ammonium-related metabolic changes affect somatic embryogenesis in pumpkin (cucurbita pepo l.).	somatic embryogenesis in pumpkin can be induced on auxin-containing medium and also on hormone-free medium containing 1mm ammonium (nh(4)(+)) as the sole source of nitrogen. growth of nh(4)(+)-induced embryogenic tissue was slow and caused considerable acidification of the culture medium. small spherical cells with dense cytoplasma formed proembryogenic cell clusters that could not develop into late stage embryos. buffering of nh(4)(+) medium with 25mm 2-(n-morpholino)-ethane-sulfonic acid enhan ...	2011	21807439
xylan-degrading enzymes in male and female flower nectar of cucurbita pepo.	nectar is a very complex mixture of substances. some components (sugars and amino acids) are considered primary alimentary rewards for animals and have been investigated and characterized in numerous species for many years. in contrast, nectar proteins have been the subject of few studies and little is known of their function. only very recently have detailed studies and characterization of nectar proteins been undertaken, and then for only a very few species. this current work represents a firs ...	2011	21813563
determination of the melon chloroplast and mitochondrial genome sequences reveals that the largest reported mitochondrial genome in plants contains a significant amount of dna having a nuclear origin.	the melon belongs to the cucurbitaceae family, whose economic importance among vegetable crops is second only to solanaceae. the melon has a small genome size (454 mb), which makes it suitable for molecular and genetic studies. despite similar nuclear and chloroplast genome sizes, cucurbits show great variation when their mitochondrial genomes are compared. the melon possesses the largest plant mitochondrial genome, as much as eight times larger than that of other cucurbits.	2011	21854637
Performance of Apis mellifera, Bombus impatiens, and Peponapis pruinosa (Hymenoptera: Apidae) as pollinators of pumpkin.	Pollination services of pumpkin, Cucurbita pepo L., provided by the European honey bee, Apis mellifera L., were compared with two native bee species, the common eastern bumble bee, Bombus impatiens (Cresson), and Peponapis pruinosa Say, in New York from 2008 to 2010. Performance of each species was determined by comparing single-visit pollen deposition, percentage of visits that contacted the stigma, flower-handling time, fruit and seed set, and fruit weight per number of visits. Fruit yield fro ...	2011	21882677
zucchini yellow mosaic virus: biological properties, detection procedures and comparison of coat protein gene sequences.	between 2006 and 2010, 5324 samples from at least 34 weed, two cultivated legume and 11 native species were collected from three cucurbit-growing areas in tropical or subtropical western australia. two new alternative hosts of zucchini yellow mosaic virus (zymv) were identified, the australian native cucurbit cucumis maderaspatanus, and the naturalised legume species rhyncosia minima. low-level (0.7%) seed transmission of zymv was found in seedlings grown from seed collected from zucchini (cucur ...	2011	21935626
microbial diversity inside pumpkins: microhabitat-specific communities display a high antagonistic potential against phytopathogens.	recent and substantial yield losses of styrian oil pumpkin (cucurbita pepo l. subsp. pepo var. styriaca greb.) are primarily caused by the ascomycetous fungus didymella bryoniae but bacterial pathogens are frequently involved as well. the diversity of endophytic microbial communities from seeds (spermosphere), roots (endorhiza), flowers (anthosphere), and fruits (carposphere) of three different pumpkin cultivars was studied to develop a biocontrol strategy. a multiphasic approach combining molec ...	2011	21947430
genetic relationships and evolution in cucurbita pepo (pumpkin, squash, gourd) as revealed by simple sequence repeat polymorphisms.	genetic relationships among 104 accessions of cucurbita pepo were assessed from polymorphisms in 134 ssr (microsatellite) and four scar loci, yielding a total of 418 alleles, distributed among all 20 linkage groups. genetic distance values were calculated, a dendrogram constructed, and principal coordinate analyses conducted. the results showed 100 of the accessions as distributed among three clusters representing each of the recognized subspecies, pepo, texana, and fraterna. the remaining four ...	2012	22101929
analysis of factors affecting volatile compound formation in roasted pumpkin seeds with selected ion flow tube-mass spectrometry (sift-ms) and sensory analysis.	pumpkin (cucurbita pepo and maxima) seeds are uniquely flavored and commonly consumed as a healthy roasted snack. the objective was to determine dominant volatiles in raw and roasted pumpkin seeds, and the effect of seed coat, moisture content, fatty acid ratio, total lipids, reducing sugars, and harvest year on volatile formation. sensory was conducted to evaluate overall liking of seed variety and texture. seed processing included extraction from the fruit, dehydration, and roasting (150 °c). ...	2012	22122232
effect of reproductive mode on host plant utilization of melon aphid (hemiptera: aphididae).	variation in the reproductive mode of melon aphid aphis gossypii glover occurred on the large geographic scale, but the performance of different reproductive modes to use host plant is poorly understood. life tables of melon aphid population that undergo the anholocyclic, androcyclic, and intermediate reproductive mode were conducted on different host plants. the results showed that the anholocyclic and androcyclic strains could become adults and produce offspring on cotton gossypium hirsutum l. ...	2010	22182553
combining ability of summer-squash lines with different degrees of parthenocarpy and prsv-w resistance.	the aim was to assess heterosis in a set of 16 summer-squash hybrids, and evaluate the combining capacity of the respective parental lines, which differed as to the degree of parthenocarpy and resistance to prsv-w (papaya ringspot virus-watermelon strain). the hybrids were obtained using a partial diallel cross design (4 × 4). the lines of parental group i were 1 = abx-037g-77-03-05-01-01-bulk, 2 = abx-037g-77-03-05-03-10-bulk, 3 = abx-037g-77-03-05-01-04-bulk and 4 = abx-037g-77-03-05-05-01-bul ...	2011	22215966
pollination value of male bees: the specialist bee peponapis pruinosa (apidae) at summer squash (cucurbita pepo).	male bees can be abundant at flowers, particularly floral hosts of those bee species whose females are taxonomic pollen specialists (oligolecty). contributions of male bees to host pollination are rarely studied directly despite their prevalence in a number of pollination guilds, including those of some crop plants. in this study, males of the oligolectic bee, peponapis pruinosa say, were shown to be effective pollinators of summer squash, cucurbita pepo l. seven sequential visits from male p. p ...	2011	22251639
high-throughput snp genotyping in cucurbita pepo for map construction and quantitative trait loci mapping.	cucurbita pepo is a member of the cucurbitaceae family, the second- most important horticultural family in terms of economic importance after solanaceae. the "summer squash" types, including zucchini and scallop, rank among the highest-valued vegetables worldwide. there are few genomic tools available for this species.the first cucurbita transcriptome, along with a large collection of single nucleotide polymorphisms (snp), was recently generated using massive sequencing. a set of 384 snp was sel ...	2012	22356647
flow injection assessment of nitrate contents in fresh and cooked fruits and vegetables grown in fiji.	nitrates form part of the essential chemistry of soils and plants. thus, plant roots are able to absorb nitrate directly from the soil. it has been discovered that human nitrate intake is mainly from vegetables. vegetables play an important role in human nutrition since they are an outstanding source for vitamins, minerals, and biologically active compounds. in risk assessment of nitrate contents, this study reports the nitrate levels of 8 commonly consumed fruits and vegetables grown in fiji, t ...	2011	22417577
shoot excision has no effect on net flux of protons, ammonium or nitrate in seedling roots of a conifer and three crop species.	a high-flux region, 5mm from the root tips of seedlings of coastal douglas-fir (pseudotsuga menziesii), soybean (glycine max), zucchini (cucurbita pepo) and pea (pisum sativum), was monitored using a microelectrode ion flux measurement system, for changes in the net fluxes of h(+), nh(4)(+) and no(3)(-) in response to shoot removal. in all species, careful excision of the seedling shoot had no significant effect on the net fluxes of h(+), nh(4)(+) or no(3)(-) measured 5mm from the root tip. expe ...	2012	22494824
isolation and characterization of 10 ssr markers of momordica charantia (cucurbitaceae).	microsatellite markers were isolated and characterized from the genome of momordica charantia (bitter melon) to be applied in studies of genetic diversity and population structure.	2012	22523346
composite cucurbita pepo plants with transgenic roots as a tool to study root development.	in most plant species, initiation of lateral root primordia occurs above the elongation zone. however, in cucurbits and some other species, lateral root primordia initiation and development takes place in the apical meristem of the parental root. composite transgenic plants obtained by agrobacterium rhizogenes-mediated transformation are known as a suitable model to study root development. the aim of the present study was to establish this transformation technique for squash.	2012	22553131
accumulation of weathered pp'-dde in xylem sap of grafted watermelon.	movement of weathered p,p'-dichlorodiphenyldichloroethane (p,p'-dde) from contaminated soil to the rhizosphere pore water to the xylem sap of grafted watermelon was studied under green house conditions. p,p'-dde concentrations in pore water and xylem sap was compared in intact plants, homografted, and compatible heterografts of cucurbita pepo spp. pepo and citrullus lanatus plants. an average p,p'-dde concentrations in pore water of contaminated soil ranged from 0.36 microg/l to 0.55 microg/l an ...	2012	22567720
accumulation and phytotoxicity of engineered nanoparticles to cucurbita pepo.	the effect of bulk and engineered nanoparticle (np) ag, au, cu, si, and c at 250 and 750 mg/l on zucchini biomass, transpiration, and element content was determined. the ph of bulk and np solutions prior to plant growth frequently differed. nanoparticle cu solution ph was significantly higher than bulk cu, whereas for ag and c, the nps had significantly lower ph. plants were unaffected by au, regardless of particle size or concentration. np ag reduced plant biomass and transpiration by 49-91% co ...	2012	22567722
deep sequencing reveals persistence of intra- and inter-host genetic diversity in natural and greenhouse populations of zucchini yellow mosaic virus.	the genetic diversity present in populations of rna viruses is likely to be strongly modulated by aspects of their life history, including mode of transmission. however, how transmission mode shapes patterns of intra- and inter-host genetic diversity, particularly when acting in combination with de novo mutation, population bottlenecks and the selection of advantageous mutations, is poorly understood. to address these issues, this study performed ultradeep sequencing of zucchini yellow mosaic vi ...	2012	22592263
accumulation of weathered p,p'-ddts in hybridized cucurbita pepo cultivars.	cucurbita pepo spp pepo (zucchini) is known as an exceptional weathered dichlorodiphenyldichloroethylene (dde) accumulator, whereas cucurbita pepo ssp ovifera (squash) is termed a nonaccumulator. experiments were conducted with hybridized zucchini and squash to assess the inheritance pattern of ddx (the sum of p,p'-dichlorodiphenyltrichloroethane [p,p'-ddt], p,p'-dichlorodiphenyldichloroethane [p,p'-ddd], and p,p'-dichlorodiphenyldichloroethylene [p,p'-dde]) accumulation potential in xylem sap a ...	2012	22610730
advertising to the enemy: enhanced floral fragrance increases beetle attraction and reduces plant reproduction.	many organisms face challenges in avoiding predation while searching for mates. for plants, emitting floral fragrances to advertise reproductive structures could increase the attraction of detrimental insects along with pollinators. very few studies have experimentally evaluated the costs and benefits of fragrance emission with explicit consideration of how plant fitness is affected by both pollinators and florivores. to determine the reproductive consequences of increasing the apparency of repr ...	2012	22624324
effect of npk fertilizer on chemical composition of pumpkin (cucurbita pepo linn.) seeds.	an investigation of the proximate composition and antioxidant profile of pumpkin seeds obtained from different levels of npk 15 : 15 : 15 compound fertilizer application at the obafemi awolowo university, ile-ife, nigeria was carried out. pumpkin seeds were grown in 2010 for two cropping seasons (may to august and august to november), and the following fertilizer rates were applied: 0, 50, 100, 150, 200, and 250 kg/ha. standard analytical methods were used to determine protein, crude fibre, ash, ...	2012	22629204
virus infections in wild plant populations are both frequent and often unapparent.	pathogens are thought to regulate host populations. in agricultural crops, virus infection reduces yield. however, in wild plants little is known about the spatial and temporal patterns of virus prevalence. thus, pathogen effects on plant population dynamics are unclear. prevalence data provide necessary background for (1) evaluating the effects of virus infection on plant population size and dynamics and (2) improving risk assessment of virus-resistant transgenic crops.	2012	22645099
anthelmintic efficacy of pumpkin seed (cucurbita pepo linnaeus, 1753) on ostrich gastrointestinal nematodes in a semiarid region of paraíba state, brazil.	the aim of this study was to verify the in vivo effectiveness of pumpkin seed (curcubita pepo linnaeus, 1753) in naturally infected ostriches in the cariri zone, semiarid region of paraíba state, brazil. forty-eight ostriches were used, african black breed, of 14 to 36 months old, naturally infected by gastrointestinal nematodes. these animals were divided into four groups of 12 ostriches. group 1 consists of animals treated with 0.5 g/kg live weight (l. w.) of pumpkin seed meal; group 2 receive ...	2013	22684690
influence of different fertilizer types of zucchini (cucurbita pepo) on the structure of nematode communities.	increasing efficiency of production of vegetable crops is directly related to search for appropriate solution to increase their productivity. organic amendments have been used for centuries to improve soil fertility and crop yield. our study suggests that organic amendments can also be used as nematicidal agents. the survey was conducted on experimental field of department horticulture at agricultural university of plovdiv, bulgaria in 2009 on nematode infested sites. combination with two types ...	2011	22696945
predominant effects of host plant species on aphis gossypii aphid and e. pisyrphus balteatus hoverfly in a tritrophic approach.	the aphid aphis gossypii glover (homoptera: aphididae) is known as worldwide important pest. it causes serious injuries to crops, but is also able to feed on various cultivated and wild plants. some programs are developped to control this aphid. in many studies, hoverfy episyrphus balteatus de geer (diptera: syrphidae) is used as an efficient and aphid specific predator in biological control systems. in this work, we study cotton aphid choice plant discrimination, the effect of host plants on it ...	2011	22696952
movement of soil-applied imidacloprid and thiamethoxam into nectar and pollen of squash (cucurbita pepo).	there has been recent interest in the threat to bees posed by the use of systemic insecticides. one concern is that systemic insecticides may translocate from the soil into pollen and nectar of plants, where they would be ingested by pollinators. this paper reports on the movement of two such systemic neonicotinoid insecticides, imidacloprid and thiamethoxam, into the pollen and nectar of flowers of squash (cucurbita pepo cultivars "multipik," "sunray" and "bush delicata") when applied to soil b ...	2012	22761727
low temperature acclimation of photosynthetic capacity and leaf morphology in the context of phloem loading type.	carbon export from leaf mesophyll to sugar-transporting phloem occurs via either an apoplastic (across the cell membrane) or symplastic (through plasmodesmatal cell wall openings) pathway. herbaceous apoplastic loaders generally exhibit an up-regulation of photosynthetic capacity in response to growth at lower temperature. however, acclimation of photosynthesis to temperature by symplastically loading species, whose geographic distribution is particularly strong in tropical and subtropical areas ...	2012	22791016
characterization of the small rna component of leaves and fruits from four different cucurbit species.	micrornas (mirnas) are a class of non-coding small rnas involved in post-transcriptional regulation of gene expression critical for plant growth and development, stress responses and other diverse biological processes in plants. the cucurbitaceae or cucurbit family represents some of economically important species, particularly those with edible and medicinal fruits. genomic tools for the molecular analysis of members of this family are just emerging. partial draft genome sequence became availab ...	2012	22823569
fullerene-enhanced accumulation of p,p'-dde in agricultural crop species.	the effect of c(60) fullerene exposure on the accumulation of dichlorodiphenyldichloroethylene (p,p'-dde; ddt metabolite) by cucurbita pepo l. (zucchini), glycine max l. (soybean), and solanum lycopersicum l. (tomato) was determined. the plants were grown in 125 ml jars of vermiculite amended with 0 or 40 mg of c(60) fullerenes. prior to planting, the jars were amended with 40 ml solution containing 100 ng/ml of p,p'-dde with 0 or 100 mg/l humic acid. during three weeks of growth, plants were wa ...	2012	22856886
nutritional and antioxidant profiles of pumpkin (cucurbita pepo linn.) immature and mature fruits as influenced by npk fertilizer.	this study evaluated the influence of npk fertilizer on protein, fibre, ash, fat, carbohydrate, antioxidant activities and antioxidant phenolic compounds in immature and mature fruits of pumpkin. the treatment consisted of six npk levels (0, 50, 100, 150, 200 and 250 kg/ha), and was replicated six times in a randomized complete block design (rcbd). proximate analysis and antioxidant assays were done using standard analytical methods. at control and lower npk rates, the proximate compositions and ...	2012	22868114
toxicity of silver and copper to cucurbita pepo: differential effects of nano and bulk-size particles.	the phytotoxicity of bulk and nanoparticle cu and ag was directly compared. np ag reduced biomass and transpiration by 66-84% when compared with bulk ag. the ag ion concentration was 4.4-10-times greater in np than bulk particle solutions. the cu ion concentration was 1.4-4.4-times greater in bulk than np amended solutions. humic acid (50 mg/l) decreased the ion content of bulk cu solution by 38-42% but increased ion cu content of np solutions by 1.4-2.9 times. bulk and np cu were highly phytoto ...	2012	22887766
development of a hull-less pumpkin (cucurbita pepo l.) seed oil press-cake spread.	a stable, oil-based spread rich in the omega-3 (ω-3) and omega-6 (ω-6) fatty acids was developed using a hull-less pumpkin seed (cucurbita pepo l.) oil press-cake, a by-product of the pumpkin oil pressing process, along with cold-pressed hemp oil. response surface methodology (rsm) was applied to investigate the effects of two factors, as the formulation's compositional variables: a commercial stabilizer (x(1) ) and cold-pressed hemp oil (x(2) ) added to the pumpkin seed oil press-cake in the sp ...	2012	22900635
the use of biochar to reduce soil pcb bioavailability to cucurbita pepo and eisenia fetida.	biochar is a carbon rich by-product produced from the thermal decomposition of organic matter under low oxygen concentrations. currently many researchers are studying the ability of biochar to improve soil quality and function in agricultural soils while sustainably sequestering carbon. this paper focuses on a novel but complimentary application of biochar - the reduced bioavailability and phytoavailability of organic contaminants in soil, specifically polychlorinated biphenyls (pcbs). in this g ...	2012	22922132
chemical composition and functional characterisation of commercial pumpkin seed oil.	pumpkin (cucurbita pepo l.) seed oil is a common product in slovenia, hungary and austria and is considered a preventive agent for various pathologies, particularly prostate diseases. these properties are related to its high content of carotenoids and liposoluble vitamins. in this study the carotenoid (lutein and zeaxanthin), vitamin e (α- and γ-tocopherol) and fatty acid contents of 12 samples of commercial pumpkin seed oil were investigated together with the composition of the volatile fractio ...	2013	22936573
pathogen effects on vegetative and floral odours mediate vector attraction and host exposure in a complex pathosystem.	pathogens can alter host phenotypes in ways that influence interactions between hosts and other organisms, including insect disease vectors. such effects have implications for pathogen transmission, as well as host exposure to secondary pathogens, but are not well studied in natural systems, particularly for plant pathogens. here, we report that the beetle-transmitted bacterial pathogen erwinia tracheiphila - which causes a fatal wilt disease - alters the foliar and floral volatile emissions of ...	2012	22988893
carbonaceous soil amendments to biofortify crop plants with zinc.	carbonaceous soil amendments, comprising mixtures of biosolids and biochar, have been demonstrated to improve fertility while reducing nitrate leaching. we aimed to determine the efficacy of a biosolids/biochar soil amendment in biofortification of vegetables with zn, an element that is deficient in one third of humanity. we grew beetroot (beta vulgaris), spinach (spinacia oleracea), radish (raphanus sativus), broccoli (brassica oleracea), carrot (daucus carota), leek (allium ampeloprsum), onion ...	2013	23146312
suitability of elemental fingerprinting for assessing the geographic origin of pumpkin (cucurbita pepo var. styriaca) seed oil.	an analytical method was developed and validated for the classification of the geographical origin of pumpkin seeds and oil from austria, china and russia. the distribution of element traces in pumpkin seed and pumpkin seed oils in relation to the geographical origin of soils of several agricultural farms in austria was studied in detail. samples from several geographic origins were taken from parts of the pumpkin, pumpkin flesh, seeds, the oil extracted from the seeds and the oil-extraction cak ...	2013	23194559
a comparative study of carrier participation in the transport of 2,3,5-triiodobenzoic acid, indole-3-acetic acid, and 2,4-dichlorophenoxyacetic acid by cucurbita pepo l. hypocotyl segments.	the transmembrane transport of indole-3-acetic acidd (iaa) and 2,4-dichlorophenoxyacetic acid (2,4-d) by 2 mm segments of dark-grown cucurbita pepo l. hypocotyls involves nonme-diated diffusion of the neutral protonated auxin species as well as carrier-mediated components for influx and efflux. the efflux carrier is blocked by 2,3,5-triiodobenzoic acid (tiba), a noncompetitive inhibitor of polar auxin transport which is itself transported in a polar fashion. the transmembrane transport of tiba i ...	1984	23194793
the involvement of endogenous cytokinin-like and gibberellin-like substances in germination and early seedling growth of cucurbita pepo l.	mature dry seeds of cucurbita pepo contained measurable quantities of both gibberellin-like and cytokinin-like materials. during germination, levels of endogenous gibberellin-like substances initially fell, but subsequently increased again. at no time, however, was there more endogenous gibberellin activity present than in the dry seeds. in terms of the amounts of gibberellin reported in other tissues, the levels in these seeds were low. most of the cytokinin-like activity present was in the for ...	1984	23195299
the effects of methanolic, chloroform, and ethylacetate extracts of the cucurbita pepo l. on the delay type hypersensitivity and antibody production.	pumpkin, as a dietary plant, has been used in traditional medicine around the world. in addition, during the last decade, antidiabetic, antihypertensive, antitumor, intestinal antiparasitic, antibacterial, anti hypercholesterolemia, anti-inflammatory, immunomodulatory and analgic effects of pumpkin has been reported. the aim of the present study was to determine the effects of different extracts of cucurbita pepo l. on the immune responses. methanolic, chloroform and ethylacetate extracts of c. ...	2012	23248672
impact of ag nanoparticle exposure on p,p'-dde bioaccumulation by cucurbita pepo (zucchini) and glycine max (soybean).	the effect of nanoparticle (np), bulk, or ionic ag exposure on dichlorodiphenyldichloroethylene (p,p'-dde; ddt metabolite) accumulation by glycine max l. (soybean) and cucurbita pepo l. (zucchini) was investigated. the plants were grown in 125-ml jars of vermiculite amended with 500 or 2000 mg/l of bulk or np ag; ion controls at 5 and 20 mg/l were established. during 19 d of growth, plants were amended with solution containing 100 ng/ml of p,p'-dde. total shoot p,p'-dde levels in non-ag exposed ...	2013	23252415
effect of c60 fullerenes on the accumulation of weathered p,p'-dde by plant and earthworm species under single and multispecies conditions.	the use of engineered nanomaterials has increased dramatically in recent years, but an understanding of nanomaterial fate and effects in the environment is lacking. in particular, the interaction of nanomaterials with coexisting organic contaminants and the subsequent implications for sensitive biota is almost completely unknown. here, the effect of c60 fullerenes on the accumulation of weathered dichlorodiphenyldichloroethylene (p,p'-dde; ddt metabolite) by cucurbita pepo (pumpkin) and eisenia ...	2013	23401244
a major latex-like protein is a key factor in crop contamination by persistent organic pollutants.	this is the first report, to our knowledge, to reveal important factors by which members of the cucurbitaceae family, such as cucumber (cucumis sativus), watermelon (citrullus lanatus), melon (cucumis melo), pumpkin (cucurbita pepo), squash (c. pepo), and zucchini (c. pepo), are selectively polluted with highly toxic hydrophobic contaminants, including organochlorine insecticides and dioxins. xylem sap of c. pepo ssp. pepo, which is a high accumulator of hydrophobic compounds, solubilized the hy ...	2013	23404917
characterization of the aroma signature of styrian pumpkin seed oil ( cucurbita pepo subsp. pepo var. styriaca) by molecular sensory science.	application of the aroma extract dilution analysis on a distillate prepared from an authentic styrian pumpkin seed oil followed by identification experiments led to the characterization of 47 odor-active compounds in the flavor dilution (fd) factor range of 8-8192 among which 2-acetyl-1-pyrroline (roasty, popcorn-like), 2-propionyl-1-pyrroline (roasty, popcorn-like), 2-methoxy-4-vinylphenol (clove-like), and phenylacetaldehyde (honey-like) showed the highest fd factors. among the set of key odor ...	2013	23461409
structural diversity of pectins isolated from the styrian oil-pumpkin (cucurbita pepo var. styriaca) fruit.	to evaluate the seeded fruit biomass of the styrian oil-pumpkin in view of its pectin component, a series of acidic polysaccharides were isolated by a six-step sequential extraction using hot water, edta, dilute hcl (twice) and dilute and stronger naoh solutions. chemical, physicochemical and spectroscopy analyses revealed that the first four fractions comprised partially methyl-esterified and acetylated pectins with varying proportions of rhamnogalacturonan regions ramified with galactose- and ...	2012	23465915
phytoaccumulation of antimicrobials by hydroponic cucurbita pepo.	consumer use of antimicrobial-containing products continuously introduces triclocarban and triclosan into the environment. triclocarban and triclosan adversely affect plants and animals and have the potential to affect human health. research examined the phytoaccumulation of triclocarban and triclosan by pumpkin (cucurbita pepo cultivar howden) and zucchini (cucurbita pepo cultivar gold rush) grown hydroponically. pumpkin and zucchini were grown in nutrient solution spiked with 0.315 microg/ml t ...	2013	23487999
characteristics of antioxidant activity and composition of pumpkin seed oils in 12 cultivars.	the objective of this study was to determine the antioxidant properties, and provide characteristics, of the oil obtained from the seeds of 12 pumpkin varieties belonging to the species cucurbita maxima duch. and cucurbita pepo l. another objective was to establish which of the two extracting agents, ethanol or methanol, is more effective. the seeds of the pumpkin varieties examined differ in chemical composition and antioxidant activity. the seeds of the cultivars belonging to the species c. ma ...	2013	23561092
heat and mass transfer in deep-frying of pumpkin, sweet potato and taro.	heat and mass transfer parameters, effective thermal diffusivity, heat transfer coefficient, effective moisture diffusivity and moisture transfer coefficient-for pumpkin (cucurbita pepo), sweet potato (ipomoea batatas) and taro (colocasia esculenta) under deep-frying conditions were determined by fitting experimental data on transient values of temperature and moisture content to the solution of the standard diffusion equation in cylindrical coordinates as modified by dincer (heat mass transfer ...	2010	23572697
a phase ii randomised double-blind placebo-controlled clinical trial investigating the efficacy and safety of prostateeze max: a herbal medicine preparation for the management of symptoms of benign prostatic hypertrophy.	the aim of the clinical trial was to evaluate the efficacy and safety of prostateeze max, an orally dosed herbal preparation containing cucurbita pepo, epilobium parviflorum, lycopene, pygeum africanum and serenoa repens in the management of symptoms of medically diagnosed benign prostate hypertrophy (bph).	2013	23642948
carotenogenic gene expression and carotenoid accumulation in three varieties of cucurbita pepo during fruit development.	the control of gene expression is a crucial regulatory mechanism in carotenoid accumulation of fruits and flowers. we investigated the role of transcriptional regulation of nine genes involved in the carotenoid biosynthesis pathway in three varieties of cucurbita pepo with evident differences in fruit color. the transcriptional levels of the key genes involved in the carotenoid biosynthesis were higher in flower-, leaf-, and fruit skin tissues than flesh tissues. this correlated with higher conc ...	2013	23773001
boron excess affects photosynthesis and antioxidant apparatus of greenhouse cucurbita pepo and cucumis sativus.	this study aimed to evaluate the behavior of zucchini (cucurbita pepo l.) and cucumber (cucumis sativus l.) under boron (b) excess. plants were grown under greenhouse conditions in a sandy soil-peat mixture using a nutrient solution containing 0.2 (control), 10 and 20 mg l(-1) b. visible symptoms were quantified and leaf b accumulation, gas exchanges, chlorophyll (chl) a fluorescence, malondialdehyde by-products and antioxidants were investigated 20 days after the beginning of the treatments. bo ...	2013	23779070
a light in the shadow: the use of lucifer yellow technique to demonstrate nectar reabsorption.	nectar reabsorption is a widely known phenomenon, related to the strategy of resource-recovery and also to maintain the nectar homeostasis at the nectary. the method currently performed to demonstrate nectar being reabsorbed involves the use of radioactive tracers applied to the nectary. although this method works perfectly, it is complex and requires specific supplies and equipment. therefore, here we propose an efficient method to obtain a visual demonstration of nectar reabsorption, adapting ...	2013	23783170
modeling the parameters for plasmodesmal sugar filtering in active symplasmic phloem loaders.	plasmodesmata (pd) play a key role in loading of sugars into the phloem. in plant species that employ the so-called active symplasmic loading strategy, sucrose that diffuses into their unique intermediary cells (ics) is converted into sugar oligomers. according to the prevalent hypothesis, the oligomers are too large to pass back through pd on the bundle sheath side, but can pass on into the sieve element to be transported in the phloem. here, we investigate if the pd at the bundle sheath-ic int ...	2013	23802006
a novel 3α-p-nitrobenzoylmultiflora-7:9(11)-diene-29-benzoate and two new triterpenoids from the seeds of zucchini (cucurbita pepo l).	three novel multiflorane-type triterpenoids, 3α-p-nitrobenzoylmultiflora-7:9(11)-diene-29-benzoate (1), 3α-acetoxymultiflora-7:9(11)-diene-29-benzoate (2), and 3α-acetoxymultiflora-5(6):7:9(11)-triene-29-benzoate (3), along with two known related compounds 4 and 5 were isolated from the seeds of zucchini (cucurbita pepo l). their structures were determined on the basis of 1d and 2d nmr spectroscopy and hreims. triterpenoids possessing a nitro group were not isolated previously.	2013	23803716
inheritance profile of weathered chlordane and p,p'-ddts accumulation by cucurbita pepo hybrids.	cucurbita pepo ssp pepo (zucchini) accumulates significant levels of persistent organic pollutants in its roots, followed by unexpectedly high contaminant translocation to the stems. most other plant species, including the closely related c. pepo ssp ovifera (squash), do not have this ability. to investigate the mechanism of contaminant accumulation, two cultivars each of parental zucchini and squash, as well as previously created first filial (f1) hybrids and f1 backcrosses (bc) of those parent ...	2013	23819281
zucchini yellow mosaic virus (zymv, potyvirus): vertical transmission, seed infection and cryptic infections.	the role played by seed transmission in the evolution and epidemiology of viral crop pathogens remains unclear. we determined the seed infection and vertical transmission rates of zucchini yellow mosaic virus (zymv), in addition to undertaking illumina sequencing of nine vertically transmitted zymv populations. we previously determined the seed-to-seedling transmission rate of zymv in cucurbita pepo ssp. texana (a wild gourd) to be 1.6%, and herein observed a similar rate (1.8%) in the subsequen ...	2013	23845301
effects of silicates from scaps of photovoltaic industries on powdery mildew of zucchini.	silicon is the second most abundant element on earth's surface and its use can stimulate natural defense mechanisms in plants. the effect of silicate from scraps of photovoltaic industries against powdery mildew on zucchini (cucurbita pepo) was evaluated under greenhouse conditions. potted plants were inoculated with a spore suspension containing 1 x 10(5) cfu/ml. the following treatments have been carried out, 3 and 10 days after pathogen inoculation: chemical fungicide (propiconazole, tilt 25 ...	2012	23878962
cloning and characterisation of a putative pollen-specific polygalacturonase gene (cppg1) differentially regulated during pollen development in zucchini (cucurbita pepo l.).	studies in zucchini (cucurbita pepo l. spp. pepo) pollen have been limited to the viability and morphology of the mature pollen grain. the enzyme polygalacturonase (pg) is involved in pollen development and pollination in many species. in this work, we study anther and pollen development of c. pepo and present the cloning and characterisation of a putative pg cppg1 (accession no. hq232488) from pollen cdna in c. pepo. the predicted protein for cppg1 has 416 amino acids, with a high homology to o ...	2014	23879260
pollination services provided by bees in pumpkin fields supplemented with either apis mellifera or bombus impatiens or not supplemented.	pollinators provide an important service in many crops. managed honey bees (apis mellifera l.) are used to supplement pollination services provided by wild bees with the assumption that they will enhance pollination, fruit set and crop yield beyond the levels provided by the wild bees. recent declines in managed honey bee populations have stimulated interest in finding alternative managed pollinators to service crops. in the eastern u.s., managed hives of the native common eastern bumble bee (bo ...	2013	23894544
in situ application of activated carbon and biochar to pcb-contaminated soil and the effects of mixing regime.	the in situ use of carbon amendments such as activated carbon (ac) and biochar to minimize the bioavailability of organic contaminants is gaining in popularity. in the first in situ experiment conducted at a canadian pcb-contaminated brownfield site, gac and two types of biochar were statistically equal at reducing pcb uptake into plants. pcb concentrations in cucurbita pepo root tissue were reduced by 74%, 72% and 64%, with the addition of 2.8% gac, burt's biochar and blueleaf biochar, respecti ...	2013	23933124
the effect of pumpkin (cucurbita pepo l) seeds and l-arginine supplementation on serum lipid concentrations in atherogenic rats.	the present study aimed to examine the effect of pumpkin (cucurbita pepo l.) seeds supplementation on atherogenic diet-induced atherosclerosis. rat were divided into two main groups , normal control and atherogenic control rats , each group composed of three subgroups one of them supplemented with 2% arginine in drinking water and the other supplemented with pumpkin seeds in diet at a concentration equivalent to 2% arginine. supplementation continued for 37 days. atherogenic rats supplemented wi ...	2012	23983330
effect of acetylation on antioxidant and cytoprotective activity of polysaccharides isolated from pumpkin (cucurbita pepo, lady godiva).	acetylation of pumpkin (cucurbita pepo, lady godiva variety) polysaccharide using acetic anhydride with pyridines as catalyst under different conditions was conducted to obtain different degrees of acetylation on a laboratory scale. furthermore, antioxidant activities and cytoprotective effects of pumpkin polysaccharide and its acetylated derivatives were investigated employing various established in vitro systems. results showed that addition of pyridine as catalyst could increase the degree of ...	2013	23987399
[effects of host plants on the life table parameters of experimental populations of aphis gossypii].	a comparative study was conducted on the life table parameters of aphis gossypii reared on five host plant species at (25 +/- 1) degrees c in laboratory. there existed significant differences in the durations of various developmental stages, adult longevity, mean offspring number per day, net reproductive rate, intrinsic rate of increase, finite rate of increase, mean generation time, and population doubling time for the a. gossypii populations reared on the host plants. for the aphids on lagena ...	2013	24015567
involvement of ethylene biosynthesis and signalling in fruit set and early fruit development in zucchini squash (cucurbita pepo l.).	we have identified a kind of parthenocarpy in zucchini squash which is associated with an incomplete andromonoecy, i.e. a partial conversion of female into bisexual flowers. given that andromonoecy in this and other cucurbit species is caused by a reduction of ethylene production in the female flower, the associated parthenocarpic development of the fruit suggested the involvement of ethylene in fruit set and early fruit development.	2013	24053311
cucurbita spp. and cucumis sativus enhance the dissipation of polychlorinated biphenyl congeners by stimulating soil microbial community development.	a number of cucurbita species have the potential to extract polychlorinated biphenyls (pcbs) from soil, but their impact on the soil microbial communities responsible for pcb degradation remains unclear. a greenhouse experiment was conducted to investigate the effect of three cucurbita and one cucumis species on pcb dissipation and soil microbial community structure. compared to the unplanted control, enhanced losses of pcbs (19.5%-42.7%) were observed in all planted soils. cucurbita pepo and cu ...	2014	24077568
multiwalled carbon nanotubes and c60 fullerenes differentially impact the accumulation of weathered pesticides in four agricultural plants.	the effect of multiwalled carbon nanotubes (mwcnt) or c60 fullerenes on the uptake of weathered chlordane or ddx (ddt + metabolites) by cucurbita pepo (zucchini), zea mays (corn), solanum lycopersicum (tomato), and glycine max (soybean) was investigated. the plants were grown in 50 g of soil with weathered chlordane (2150 ng/g) and ddx (118 ng/g) that was amended with 0, 500, 1000, or 5000 mg/kg mwcnt or c60. after 28 d, the root and shoot content of chlordane components and ddx was determined b ...	2013	24079803
disease interactions in a shared host plant: effects of pre-existing viral infection on cucurbit plant defense responses and resistance to bacterial wilt disease.	both biotic and abiotic stressors can elicit broad-spectrum plant resistance against subsequent pathogen challenges. however, we currently have little understanding of how such effects influence broader aspects of disease ecology and epidemiology in natural environments where plants interact with multiple antagonists simultaneously. in previous work, we have shown that healthy wild gourd plants (cucurbita pepo ssp. texana) contract a fatal bacterial wilt infection (caused by erwinia tracheiphila ...	2013	24155951
[influence of a variation potential on photosynthesis in pumpkin seedlings (cucurbita pepo l.)].	the influence of a variation potential on photosynthesis in pumpkin seedlings (cucurbita pepo l.) was investigated in our work. it was shown that the variation potential induced by cotyledon burning propagates into a leaf. it decreases co2 assimilation and transpiration as well as increases nonphotochemical quenching. investigation of isolated chloroplasts showed that lowering of the ph in incubation medium from 6.9-7.2 to 6.5 increases nonphotochemical quenching. it was proposed that lowering o ...	2015	24159815
effects of pollen selection on progeny vigor in a cucurbita pepo x c. texana hybrid.	we examined the effects of pollen selection for rapid pollen-tube growth on progeny vigor. first, we crossed a wild gourd (cucurbita texana) to a cultivated zucchini (cucurbita pepo cv 'black beauty') to produce an f1 and then an f2 generation. half of the f1 seeds were produced by depositing small loads of c. texana pollen onto the stigmas of c. pepo. these small pollen loads were insufficient to produce a full complement of seeds and, consequently, both the fast- and the slow-growing pollen tu ...	1996	24166555
phytotropin-binding sites and auxin transport in cucurbita pepo: evidence for two recognition sites.	two properties of phytotropins, their ability to bind to 1-n-naphthylphthalamic acid (npa) receptors located on microsomal vesicles isolated from cucurbita pepo l. hypocotyls, and to stimulate auxin (indol-3-yl acetic acid, iaa) accumulation into such vesicles by blocking its efflux from them, were assessed in double labelling experiments using [2,3,4,5-(3)h]1-n-naphthylphthalamic acid and 3-indolyl-[2-(14)c]acetic acid. two sites of differing affinities and activities on iaa accumulation were f ...	1992	24178053
localization of galactinol, raffinose, and stachyose synthesis in cucurbita pepo leaves.	the biochemical pathway of stachyose synthesis was localized by immunocytochemical and (14)c-labeling techniques in mature cucurbita pepo l. leaves. galactinol synthase (gas; ec 2.4.1.123), the first unique enzyme in this pathway, was immunolocalized within the intermediary cells of minor veins in conventionally fixed and cryo-fixed, resin-embedded sections using polyclonal anti-gas antibodies and protein a-gold. intermediary cells are specialized companion cells with extensive symplastic connec ...	1992	24178325
the effects of calcium deficiency on cucurbita pepo l. hypocotyl cells: a (31)p nuclear-magnetic-resonance study.	calcium deficiency in zucchini (cucurbita pepo l.) is associated with reduced growth and a reduced ability to transport auxin (allan and rubery, 1991, planta 183, 604-612). an investigation of the effects of calcium-deficiency on zucchini hypocotyl cells was made using weak-acid uptake and (31)p-nuclear-magneticresonance ((31)p-nmr) spectroscopy in vivo and in tissue extracts. calcium-deficient tissue had the same cytoplasmic and vacuolar phs as normal tissue when extracellular ph was near neutr ...	1993	24178486
stimulation by auxin of phospholipase a in membrane vesicles from an auxin-sensitive tissue is mediated by an auxin receptor.	microsomal vesicles were prepared from zucchini (cucurbita pepo l.) hypocotyls containing radioactive phosphatidylethanolamine or phosphatidylcholine, and these lipids were used as substrates by phospholipase a which is activated by auxins. phospholipase d and phospholipase c hydrolysed the same substrates but were not influenced by auxin. phospholipase a was activated by the auxins indolyl-3-acetic acid, 2,4-dichlorophenoxyacetic acid and, to a lesser extent, by α-naphthaleneacetic acid whereas ...	1991	24186343
subthreshold changes in excitable membranes of cucurbits pepo l. stem cells during cooling-induced action-potential generation.	the nature of subthreshold changes in excitable plasma membranes has been investigated in stem parenchyma cells of cucurbita pepo l. during action-potential generation induced by gradual cooling (from 23 to 10 ° c). the character of the subthreshold depolarization of excitable cells is shown to be mainly defined by a decrease in the activity of the plasma-membrane electrogenie pump (h(+)-atpase). in its turn, the pump activity is controlled by thermal changes in the structure of the membrane lip ...	1992	24186654
operation of the xanthophyll cycle in higher plants in response to diurnal changes in incident sunlight.	changes in the carotenoid composition of leaves in response to diurnal changes in sunlight were determined in the crop species helianthus annuus l. (sunflower), cucurbita pepo l. (pumpkin), and cucumls sativus l. (cucumber), in the diaheliotropic mesophyte malva neglecta wallr., and in the perennial shrub euonymus kiautschovicus loesner. large daily changes were observed in the relative proportions of the components of the xanthophyll cycle, violaxanthin (v), antheraxanthin (a), and zeaxanthin ( ...	1992	24186736
proton-transport activity, sidedness, and morphometry of tonoplast and plasma-membrane vesicles purified by free-flow electrophoresis from roots of lepidium sativum l. and hypocotyls of cucurbita pepo l.	large-scale preparations of highly purified tonoplast and plasma-membrane vesicles were obtained from roots (garden cress, lepidium sativum l.) and shoots (etiolated zucchini hypocotyl, cucurbita pepo l.) of representative dicotyledonous seedlings. when tonoplast-enriched fractions of cress roots were prepared by centrifugation and then subjected to free-flow electrophoresis a highly purified tonoplast fraction was obtained. this fraction from cress roots was characterized by morphometry of fili ...	1992	24186777
flavonoids and flavonoid sulphates as probes of auxin-transport regulation in cucurbita pepo hypocotyl segments and vesicles.	the accumulation of iaa by sealed microsomal vesicles prepared from hypocotyls of dark-grown cucurbita pepo l. (zucchini) seedlings was stimulated by n-1-naphthylphthalamic acid (npa: an inhibitor of carrier-mediated auxin efflux and hence of polar auxin transport) as well as by quercetin and certain other flavonoids with a specificity pattern similar to that previously shown for their npa-like effects on auxin transport and inhibition of npa binding to saturable sites. in contrast, putatively n ...	1992	24186794
degradation of direct azo dye by cucurbita pepo free and immobilized peroxidase.	enzymatic decolourization of the azo dye, direct yellow (dy106) by cucurbita pepo (courgette) peroxidase (cp) is a complex process, which is greatly affected by ph, temperature, enzyme activity and the concentrations of h2o2 and dye. courgette peroxidase was extracted and its performance was evaluated by using the free-cp (fcp) and immobilized-cp (icp) forms in the decolourization of dy106. immobilization of peroxidase in calcium alginate beads was performed according to a strategy aiming to min ...	2013	24191614