Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| evidence of mycoplasma hyopneumoniae infection among slaughtered pigs in sri lanka. | 2005 | 15729894 | |
| enzootic pneumonia of pigs--a diagnostic dilemma. | in an enzootic pneumonia-free australian pig herd, an outbreak of a severe respiratory disease in the grow-out herd was initially diagnosed as acute tracheitis and pneumonia precipitated by the dusty environment, with a superimposed mixed infection of pasteurella multocida and arcanobacterium pyogenes. culture for mycoplasma hyopneumoniae, salmonella sp and fungi was negative. the outbreak persisted. subsequently, gross lesions consistent with enzootic pneumonia occurred, but histological lesion ... | 2005 | 15825621 |
| evaluation of mycoplasma hyopneumoniae growth by flow cytometry. | in the present study we evaluated the potential application of the flow cytometry (fc) technique to determine the growth rates of mycoplasma hyopneumoniae in a broth medium. | 2005 | 15836473 |
| efficacy of in-feed medication with tylosin for the treatment and control of mycoplasma hyopneumoniae infections. | the efficacy of in-feed medication with tylosin for the treatment of enzootic pneumonia was examined in an experimental mycoplasma hyopneumoniae infection model. one group of 10 conventional m. hyopneumoniae-free pigs was inoculated intratracheally with a highly virulent field isolate of m. hyopneumoniae; a second group of 10 pigs was inoculated in the same way and after 12 days was given tylosin at 100 mg/kg feed for 21 days; a third group of 10 pigs was inoculated with sterile culture medium, ... | 2005 | 15879541 |
| the diversity of mycoplasma hyopneumoniae within and between herds using pulsed-field gel electrophoresis. | over the years, pulsed-field gel electrophoresis (pfge) has been proven a robust technique to type isolates with a high resolution and a good reproducibility. in this study, a pfge protocol is described for the typing of mycoplasma hyopneumoniae isolates. the potential of this technique was demonstrated by comparing m. hyopneumoniae isolates obtained from the same as well as from different herds. the use of two different restriction enzymes, sali and apai, was evaluated. for each enzyme, the res ... | 2005 | 15963665 |
| diagnosis of mycoplasma hyopneumoniae. | mycoplasma hyopneumoniae, the cause of enzootic pneumonia, remains an important pathogen in the swine industry. this small, complex organism colonizes the ciliated cells of the respiratory tract, resulting in little exposure to the immune system. confirming the presence of m. hyopneumoniae, as well as identifying its role in respiratory disease and pneumonia, remains challenging to the veterinary profession. while culture of the organism remains the gold standard for identification, the use of s ... | 2004 | 15984347 |
| the occurrence of mycoplasmas in the lungs of pigs in new zealand. | attempts were made to recover and serologically identify mycoplasmas from the lungs of 50 pigs with mycoplasmal pneumonia and from 50 lungs without gross evidence of pneumonia. mycoplasma hyopneumoniae and m. hyorhinis were respectively cultured from 30% and 50% of pneumonic lungs and the former species was also recovered from 12% of non-pneumonic lungs. three other isolates (one from pneumonic and two from non-pneumonic lungs) differed in colonial morphology from m. hyopneumoniae and m. hyorhin ... | 1985 | 16031118 |
| development of nested polymerase chain reaction for the detection of mycoplasma hyopneumoniae in formalin-fixed paraffin-embedded lung tissue. | 2005 | 16035187 | |
| evaluation of the aerosol transmission of a mixed infection of mycoplasma hyopneumoniae and porcine reproductive and respiratory syndrome virus. | to evaluate the transmission of mycoplasma hyopneumoniae and porcine reproductive and respiratory syndrome virus (prrsv) by aerosol as either a single or mixed infection, 28 pigs were inoculated intratracheally with m hyopneumoniae on day 0 and infected intranasally with prrsv on day 35; they were housed together in a barn. to assess the aerosol transmission of m hyopneumoniae as a single infection, one trailer (a) containing 10 five-week-old sentinel pigs was placed along the south side of the ... | 2005 | 16040942 |
| the effect of polyclonal antibody on intracellular calcium increase induced by mycoplasma hyopneumoniae in porcine tracheal cells. | an investigation was undertaken to assess whether polyclonal convalescent and hyperimmune sera obtained from pigs inhibit mycoplasma hyopneumoniae induced increases in intracellular calcium [ca2+](i) in ciliated porcine tracheal cells. basal [ca2+](i) in the tracheal cells was 97+/-13 nm (n=22 cells in four experiments) and after exposure to m. hyopneumoniae (300 micro g/ml or 10(11) ccu/ml), [ca2+](i) increased by 246+/-56 nm within 100 s. after pre-treatment with hyperimmune or convalescent se ... | 2006 | 16051507 |
| epitope mapping of mycoplasma hyopneumoniae using phage displayed peptide libraries and the immune responses of the selected phagotopes. | phage display techniques have been widely employed to map the epitope structures which served as the basis for developing molecular vaccines. in the present study, we applied this technique to map the epitopes of mycoplasma hyopneumoniae, the etiologic agent causing swine enzootic pneumonia, and evaluated directly the immune responses in mice of the selected phage-displayed epitopes (phagotopes). two phage-displayed random peptide libraries were biopanned with the protein a-purified igg of the r ... | 2005 | 16054642 |
| swine and poultry pathogens: the complete genome sequences of two strains of mycoplasma hyopneumoniae and a strain of mycoplasma synoviae. | this work reports the results of analyses of three complete mycoplasma genomes, a pathogenic (7448) and a nonpathogenic (j) strain of the swine pathogen mycoplasma hyopneumoniae and a strain of the avian pathogen mycoplasma synoviae; the genome sizes of the three strains were 920,079 bp, 897,405 bp, and 799,476 bp, respectively. these genomes were compared with other sequenced mycoplasma genomes reported in the literature to examine several aspects of mycoplasma evolution. strain-specific region ... | 2005 | 16077101 |
| evaluation of tulathromycin for the treatment of pneumonia following experimental infection of swine with mycoplasma hyopneumoniae. | tulathromycin was evaluated in the treatment of pneumonia in weaned pigs inoculated intranasally with mycoplasma hyopneumoniae. five days postchallenge, the pigs were randomized to treatment with a single im administration of saline, a single im administration of tulathromycin (2.5 mg/kg; day 0), or three im administrations of enrofloxacin (5.0 mg/kg; days 0, 1, 2). pigs were necropsied on day 12 or 13. unchallenged controls remained healthy with no lung pathology. compared with saline, coughing ... | 2005 | 16094566 |
| evaluation of the therapeutic activity of tulathromycin against swine respiratory disease on farms in europe. | the clinical efficacy of tulathromycin in the treatment of natural outbreaks of swine respiratory disease (srd) was evaluated at five european sites. pigs (1 to 6 months of age) exhibiting clinical signs of srd were treated intramuscularly with tulathromycin (n = 247) at 2.5 mg/kg on day 0 versus either tiamulin (n = 102) at 15 mg/kg on days 0, 1, and 2 (germany, the netherlands, and the united kingdom) or florfenicol (n = 20) at 15 mg/kg on days 0 and 2 (france). actinobacillus pleuropneumoniae ... | 2005 | 16094567 |
| efficacy of tulathromycin injectable solution for the treatment of naturally occurring swine respiratory disease. | tulathromycin, a novel triamilide antimicrobial, was evaluated for treatment of swine respiratory disease (srd) in field efficacy studies involving 720 pigs in six north american swine herds. in each study, feeder pigs with clinical srd were randomly assigned in equal numbers to a group treated with tulathromycin given as a single injection at 2.5 mg/kg of body weight or to a saline-treated control group. four of the studies included a third group treated with ceftiofur sodium for 3 consecutive ... | 2005 | 16094568 |
| occurrence of salmonella serotype typhimurium dt104 on a commercial swine farm before, during, and after depopulation and repopulation. | to determine whether depopulation-repopulation could be used to eradicate salmonella serotype typhimurium dt104 from a commercial swine farm in the midwestern united states. | 2005 | 16121615 |
| [vaccination of sows against mycoplasma hyopneumoniae with hyoresp]. | the aim of the study was to investigate the serological reactions of pregnant sows to vaccination with hyoresp. further investigations were performed in the offspring of these sows to follow the dynamics of maternal antibodies and the reaction to vaccination at different points in time. the study was conducted in three farrow-to-finish herds endemically infected with m. hyopneumoniae. a total of 30 gilts and 31 sows were vaccinated 8 and 4 weeks ante partum with hyoresp (merial gmbh) or given ph ... | 2005 | 16124699 |
| comparative efficacy of two single-dose bacterins in the control of mycoplasma hyopneumoniae in swine raised under commercial conditions in brazil. | a field study was conducted in brazil to evaluate the efficacy of single vaccination of pigs with two bacterins to prevent mycoplasma hyopneumoniae lung lesions. the first (t1) treatment group (174 pigs) was injected with 2 ml of saline solution; group t2 (177 pigs) with 2 ml of bacterin a, and group t3 (174 pigs) with 2 ml of bacterin b. on days-on-test (dot) 0, 35, 66, 97 and 125, blood samples and tonsil swabs were collected from selected pigs for antibody determination (indirect elisa) and p ... | 2006 | 16129636 |
| detection of mycoplasma hyopneumoniae in lung and nasal swab samples from pigs by nested pcr and culture methods. | we examined nasal swab and lung homogenate samples collected from pigs experimentally and naturally infected with mycoplasma hyopneumoniae for the detection of m. hyopneumoniae by the nested pcr (npcr) and culture methods. in the 23 experimentally infected pigs, m. hyopneumoniae was commonly detected in nasal swabs by the npcr and culture methods at 4 weeks after inoculation, and there was a significant correlation (p<0.01) between the titers of viable organisms in nasal swabs and in lung homoge ... | 2005 | 16141667 |
| protein and antigenic variability among mycoplasma hyopneumoniae strains by sds-page and immunoblot. | porcine enzootic pneumonia (pep), with mycoplasma hyopneumoniae as the primary agent, is a chronic respiratory disease that causes major economic losses to the pig industry worldwide. the aim of this work was to analyse 18 field strains of m. hyopneumoniae isolated in gran canaria (spain) and the reference m. hyopneumoniae strain by sds-page and immunoblot. a monoclonal antibody (mab) against the membrane protein p46 reacted with all the strains in this study. in contrast, a purified polyclonal ... | 2005 | 16142605 |
| quantification of mycoplasmas in broth medium with sybr green-i and flow cytometry. | mycoplasmas are the smallest and simplest organisms known. they form a large group of bacteria that can infect humans, animals, and plants. even though several techniques have been proposed to enumerate mycoplasmas in broth medium, the determination of mycoplasma growth still remains a difficult task. the potential of using flow cytometry (fc) for rapidly estimating several species of mycoplasmas, m. agalactiae (ma), m. putrefaciens (mp), m. capricolum subsp. capricolum (mcc), m. bovis (mb), m. ... | 2006 | 16146746 |
| [field validation of a real-time pcr test for the detection of mycoplasma hyopneumoniae in nasal swabs of live pigs]. | enzootic pneumonia (ep) of pigs, caused by mycoplasma hyopneumoniae has been a notifiable disease in switzerland since may 2003. the diagnosis of ep has been based on multiple methods, including clinical, bacteriological and epidemiological findings as well as pathological examination of lungs (mosaic diagnosis). with the recent development of a real-time pcr (rtpcr) assay with 2 target sequences a new detection method for m. hyopneumoniae became available. this assay was tested for its applicab ... | 2005 | 16180340 |
| dynamics and persistence of mycoplasma hyopneumoniae infection in pigs. | the purpose of this study was to describe the dynamics (shedding and transmission) of mycoplasma hyopneumoniae infection within a population of swine and to determine the duration of the infection (persistence) through the identification of the agent in bronchial samples. sixty-three 2-month-old pigs were used in this study. the pigs (n = 28) were experimentally infected by the intratracheal route with m. hyopneumoniae and considered as seeder pigs. the remaining pigs (n = 32) were not inoculate ... | 2005 | 16187553 |
| characterization of in vivo acquired resistance of mycoplasma hyopneumoniae to macrolides and lincosamides. | macrolides and related antibiotics are used to control mycoplasma infections in the pig industry worldwide. some porcine mycoplasmas, however, survive these treatments by acquiring resistance. the mechanism of acquired resistance to macrolides and lincosamides was studied in more detail for mycoplasma hyopneumoniae by comparing both the phenotype and genotype of a resistant field isolate to five susceptible isolates. the mics were significantly higher for the resistant strain for all antibiotics ... | 2005 | 16201934 |
| in vivo expression analysis of the p97 and p102 paralog families of mycoplasma hyopneumoniae. | the p97 adhesin and p102 genes of mycoplasma hyopneumoniae each have six paralogs in the genome. we tested whether these genes were expressed during infection. p102 is associated with the mycoplasma and with swine cilia. further, most of the paralogs were transcribed in vivo in two gene transcriptional units. | 2005 | 16239586 |
| cytokine expression in porcine lungs experimentally infected with mycoplasma hyopneumoniae. | to gain further insight into the pathogenesis of porcine enzootic pneumonia (pep), cytokine expression in different pulmonary compartments was examined. mycoplasma hyopneumoniae (mh) and proinflammatory and immunoregulatory cytokines (il-1alpha, il-1beta, il-2, il-4, il-6, il-8, il-10 and tnf-alpha) were detected by immunohistochemical methods in porcine lungs experimentally infected with mh. ten pigs were inoculated intranasally with mh and killed in pairs weekly from 1- to 5-week post-inoculat ... | 2006 | 16325264 |
| expression of inflammatory cytokines in pigs experimentally infected with mycoplasma hyopneumoniae. | the expression of interleukin-1 (il-1), tumour necrosis factor-alpha (tnf-alpha) and il-6 were studied over a period of 35 days in the lungs of pigs experimentally infected with mycoplasma hyopneumoniae, by semiquantitative reverse transcription-polymerase chain reaction (rt-pcr), morphometric analysis and in-situ hybridization. fifteen colostrum-deprived pigs aged 14 days were inoculated intranasally with m. hyopneumoniae. il-1, tnf-alpha and il-6 were detected by rt-pcr in the lungs of the inf ... | 2006 | 16325841 |
| pk and pk/pd of doxycycline in drinking water after therapeutic use in pigs. | a commercial doxycycline formulation was administered in drinking water to 12 pigs at the recommended dose of 10 mg/kg daily for 5 days. the mean plasma concentration at steady-state was 1.37 +/- 1.21 microg/ml, which was reached at 68 +/- 27.2 h postadministration. absorption and elimination half-life values were 7.20 +/- 2.42 and 7.01 +/- 2.10 h, respectively. most plasma concentrations during dosing were higher than the minimum inhibitory concentrations (mics) described for the main porcine b ... | 2005 | 16343284 |
| transcriptional profiling of mycoplasma hyopneumoniae during heat shock using microarrays. | bacterial pathogens undergo stress during host colonization and disease processes. these stresses result in changes in gene expression to compensate for potentially lethal environments developed in the host during disease. mycoplasma hyopneumoniae colonizes the swine epithelium and causes a pneumonia that predisposes the host to enhanced disease from other pathogens. how m. hyopneumoniae responds to changing environments in the respiratory tract during disease progression is not known. in fact, ... | 2006 | 16368969 |
| two domains within the mycoplasma hyopneumoniae cilium adhesin bind heparin. | mycoplasma hyopneumoniae is the causative agent of porcine enzootic pneumonia, a chronic and economically significant respiratory disease that affects swine production worldwide. m. hyopneumoniae adheres to and adversely affects the function of ciliated epithelial cells of the respiratory tract, and the cilium adhesin (mhp183, p97) is intricately but not exclusively involved in this process. although binding of pathogenic bacteria to glycosaminoglycans is a recognized step in pathogenesis, knowl ... | 2006 | 16369004 |
| comparative immunogenicity of m. hyopneumoniae nrdf encoded in different expression systems delivered orally via attenuated s. typhimurium aroa in mice. | the mycoplasma hyopneumoniae ribonucleotide reductase r2 subunit (nrdf) gene fragment was cloned into eukaryotic and prokaryotic expression vectors and its immunogenicity evaluated in mice immunized orally with attenuated salmonella typhimurium aroa cs332 harboring either of the recombinant expression plasmids. we found that nrdf is highly conserved among m. hyopneumoniae strains. the immunogenicity of nrdf was examined by analyzing antibody responses in sera and lung washes, and the cell-mediat ... | 2006 | 16426773 |
| a multiplex pcr to identify porcine mycoplasmas present in broth cultures. | mycoplasma hyopneumoniae, mycoplasma hyorhinis and mycoplasma flocculare can be present in the lungs of pigs at the same time. these three mycoplasma species all require similar growth conditions and can be recovered from clinical samples using the same media. we have developed a multiplex pcr as a helpful tool for rapid differentiation of these three species in the course of isolation. based on the 16s ribosomal dna sequences, three different forward primers and a single reverse primer were sel ... | 2006 | 16437299 |
| comparison of molecular techniques for the typing of mycoplasma hyopneumoniae isolates. | in this study, we compared the potential of amplified fragment length polymorphism (aflp), random amplified polymorphic dna (rapd) analysis, restriction fragment length polymorphism (rflp) of the gene encoding lipoprotein p146, and the variable number of tandem repeats (vntr) of the p97 encoding gene, as possible methods for typing an international collection of mycoplasma hyopneumoniae isolates. all techniques showed a typeability of 100% and high intraspecific diversity. however, the discrimin ... | 2006 | 16458375 |
| a serological survey of selected pathogens in wild boar in slovenia. | serum samples collected from 178 shot wild boars (sus scrofa) were tested for the presence of antibodies against classical swine fever virus, aujeszky's disease virus (adv), porcine reproductive and respiratory syndrome virus, porcine respiratory coronavirus (prcv), transmissible gastroenteritis virus, swine influenza virus, porcine parvovirus (ppv), swine vesicular disease virus, actinobacillus pleuropneumoniae (app), mycoplasma hyopneumoniae, salmonella spp., brucella spp. and haemophilus para ... | 2006 | 16460352 |
| effects of the timing of the administration of mycoplasma hyopneumoniae bacterin on the development of lesions associated with porcine circovirus type 2. | to determine whether there is an effect of the timing of vaccination on porcine circovirus type 2 (pcv-2) replication and pcv-2-associated lesions, 78 pigs were randomly assigned to eight groups: group 1 (10 pigs) was vaccinated with a commercial mycoplasma hyopneumoniae vaccine at two and four weeks of age, group 2 (nine pigs) was vaccinated at four and six weeks of age, group 3 (10 pigs) at six and eight weeks of age and group 4 (10 pigs) at eight and 10 weeks of age; group 5 (nine pigs) was v ... | 2006 | 16461621 |
| plasmid-mediated growth hormone-releasing hormone efficacy in reducing disease associated with mycoplasma hyopneumoniae and porcine reproductive and respiratory syndrome virus infection. | the purpose of this study was to determine the effects of plasmid-mediated growth hormone releasing hormone (ghrh) supplementation on the clinical outcomes of pigs vaccinated against and challenged with either mycoplasma hyopneumonia (m. hyo) and/or with porcine reproductive and respiratory syndrome (prrs) virus. before the first vaccination, pigs received a single i.m. injection of 0.625 mg of a porcine ghrh-expressing plasmid followed by electroporation of the injection site. pigs were vaccina ... | 2006 | 16478966 |
| evaluation of the effects of animal age, concurrent bacterial infection, and pathogenicity of porcine reproductive and respiratory syndrome virus on virus concentration in pigs. | to evaluate the influences of animal age, bacterial coinfection, and porcine reproductive and respiratory syndrome virus (prrsv) isolate pathogenicity on virus concentration in pigs. | 2006 | 16506914 |
| effect of outdoor pig production on the seroprevalence of mycoplasma hyopneumoniae and lung lesions. | 2006 | 16517823 | |
| mycoplasma hyorhinis in taiwan: diagnosis and isolation of swine pneumonia pathogen. | this study attempted to determine whether one multiplex polymerase chain reaction (pcr) is an effective adjunct method for diagnosing mycoplasma hyopneumoniae and mycoplasma hyorhinis infection, and whether m. hyorhinis should be considered as an enzootic pneumonia or porcine respiratory disease complex pathogen in taiwan. to our knowledge, this study is the first to isolate and identify m. hyorhinis as a porcine pathogen in taiwan. a novel isolation method and a multiplex pcr test were applied ... | 2006 | 16540266 |
| transcriptional profiling of mycoplasma hyopneumoniae during iron depletion using microarrays. | mycoplasma hyopneumoniae, the causative agent of swine enzootic pneumonia and a major component of the porcine respiratory disease complex, continues to confound swine producers despite control programmes worldwide. the disease is chronic and self-limiting, but the host is subject to immunopathological changes that potentiate respiratory disease associated with other pathogens. the response of m. hyopneumoniae to environmental stress is of interest because of its relevance to virulence mechanism ... | 2006 | 16549658 |
| western blot immunoassay as a confirmatory test for the presence of anti-mycoplasma hyopneumoniae antibodies in swine serum. | a western blot immunoassay (wbi) was developed as a confirmatory test for 2 commercial mycoplasma hyopneumoniae (mhyo) elisas. the wbi detected at least 5 antigen bands (150, 130, 74, 70, and 30 kda) from mhyo whole membrane proteins that were not present in the antigens prepared from m. hyorhinis and m. hyosynoviae. among discrepant sera from vaccinated pigs (n = 17) and field samples (n = 91) assayed by wbi: 1) 2 of the elisa-positive samples reacted with all 5 antigens bands; 2) all blocking ... | 2006 | 16617702 |
| analysis of distribution indicates diverse functions of simple sequence repeats in mycoplasma genomes. | simple sequence repeats (ssrs) composed of extensive tandem iterations of a single nucleotide or a short oligonucleotide are rare in most bacterial genomes, but they are common among mycoplasma. some of these repeats act as contingency loci in association with families of surface antigens. by contraction or expansion during replication, these ssrs increase genetic variance of the population and facilitate avoidance of the immune response of the host. occurrence and distribution of ssrs are analy ... | 2006 | 16618962 |
| serodiagnostic comparison between two methods, elisa and surface plasmon resonance for the detection of antibody titres of mycoplasma hyopneumoniae. | a protein chip based on surface plasmon resonance (spr) was developed for measuring the mycoplasma hyopneumoniae antibody titres using a recombinant 30-kda fragment of p97 adhesin as an antigen. the diagnostic potential of this spr assay, for detecting the antibody titres to the m. hyopneumoniae 30-kda protein, was compared with that of conventional elisa using 70 pig serum samples taken from six pig farms. the spr assay was found to be highly specific and sensitive. moreover, there was a strong ... | 2006 | 16626406 |
| p159 is a proteolytically processed, surface adhesin of mycoplasma hyopneumoniae: defined domains of p159 bind heparin and promote adherence to eukaryote cells. | mycoplasma hyopneumoniae, the causative agent of porcine enzootic pneumonia, colonizes the respiratory cilia of affected swine causing significant economic losses to swine production worldwide. heparin is known to inhibit adherence of m. hyopneumoniae to porcine respiratory epithelial cilia. m. hyopneumoniae cells bind heparin but the identity of the heparin-binding proteins is limited. proteomic analysis of m. hyopneumoniae lysates identified 27 kda (p27), 110 kda (p110) and 52 kda (p52) protei ... | 2006 | 16629669 |
| identification and immunological characterization of conserved mycoplasma hyopneumoniae lipoproteins mhp378 and mhp651. | mycoplasma hyopneumoniae, the etiological agent of swine enzootic pneumonia, is an important pathogen in the swine industry worldwide. investigations on pathogenicity mechanisms as well as current serological detection methods and the development of new recombinant subunit vaccines are hampered by the lack of known and well characterized, species-specific m. hyopneumoniae antigens. as a first step to solve these problems membrane and membrane-associated proteins were enriched from m. hyopneumoni ... | 2006 | 16650945 |
| persistence of mycoplasma hyopneumoniae in experimentally infected pigs after marbofloxacin treatment and detection of mutations in the parc gene. | the ability of mycoplasma hyopneumoniae to persist despite fluoroquinolone treatments was investigated with pigs. groups of specific-pathogen-free pigs were experimentally infected with m. hyopneumoniae strain 116 and treated with marbofloxacin at the therapeutic dose (td) or half of the therapeutic dose (td/2) for 3 days. results showed that, despite tissue penetration of marbofloxacin, particularly in the trachea and the tracheal secretions, the treatments did not have any influence on m. hyop ... | 2006 | 16723552 |
| a recombinant chimera composed of r1 repeat region of mycoplasma hyopneumoniae p97 adhesin with escherichia coli heat-labile enterotoxin b subunit elicits immune response in mice. | swine mycoplasmal pneumonia (smp), caused by fastidious bacterium mycoplasma hyopneumoniae, is the most important respiratory disease in swine breeding. the commonly used vaccines to control this disease consist of inactivated whole cells (bacterins), whose production cost is high and the efficiency is limited. the objective of this study was to develop and to evaluate in balb/c mice a recombinant subunit vaccine (rltbr1) containing the r1 region of p97 adhesin of m. hyopneumoniae (r1) fused to ... | 2006 | 16730864 |
| variable number of tandem aminoacid repeats in adhesion-related cds products in mycoplasma hyopneumoniae strains. | the mycoplasma hyopneumoniae genome contains at least 22 regions with a variable number of tandem nucleotide repeats (vntrs) within coding dna sequences (cdss). in this work, the vntr-containing cdss were analysed in order to evaluate their degree of variation, possible correlations with antigenic properties, and their potential to be used as a basis for a strain typing pcr assay. we have analysed the vntrs in five m. hyopneumoniae strains (j, 7448, 7422, pms, and 232), based on published genomi ... | 2006 | 16730926 |
| antibody response to mycoplasma hyopneumoniae infection in vaccinated pigs with or without maternal antibodies induced by sow vaccination. | vaccination with bacterins is an important tool for the control of mycoplasma hyopneumoniae infection of pigs. because such vaccination often involves piglets that have suckled m. hyopneumoniae antibody-positive dams it is important to understand the effect of pre-existing (passively acquired) antibody on vaccine-induced immunity. to investigate this issue experimentally, 20 sows that were seronegative for m. hyopneumoniae were selected from a m. hyopneumoniae-infected herd and then randomly all ... | 2006 | 16732881 |
| evaluation of the immunogenicity of the p97r1 adhesin of mycoplasma hyopneumoniae as a mucosal vaccine in mice. | the immunogenicity of p97 adhesin repeat region r1 (p97r1) of mycoplasma hyopneumoniae, an important pathogenesis-associated region of p97, was evaluated in mice as a mucosal vaccine. mice were immunized orally with attenuated salmonella typhimurium aroa strain cs332 harbouring a eukaryotic or prokaryotic expression vector encoding p97r1. local and systemic immune responses were analysed by elisa on mouse sera, lung washes and splenocyte supernatants following splenocyte stimulation with specifi ... | 2006 | 16772421 |
| comparison of transmission of mycoplasma hyopneumoniae in vaccinated and non-vaccinated populations. | a transmission experiment was performed to quantify the effect of vaccination on the transmission of mycoplasma hyopneumoniae (m. hyopneumoniae) in nursery piglets by means of an adjusted reproduction ratio (r(n)). thirty piglets, vaccinated at 1 week of age, and 30 non-vaccinated piglets, free of m. hyopneumoniae, were housed in six separate pens. in each pen, three animals that were intratracheally inoculated with m. hyopneumoniae, were housed together with seven contact piglets during the con ... | 2006 | 16934376 |
| il-18 expression in pigs following infection with mycoplasma hyopneumoniae. | little is known about the detail of the immune response during infection of pigs with mycoplasma hyopneumoniae (mhp). to further understand this important porcine pathogen, we examined the interleukin-18 (il- 18) response in experimentally infected piglets. we found that large amounts of il-18 were produced in the bronchoalveolar lavage fluids (balf) of pigs experimentally infected with mhp. however, the concentration of interferon-gamma (ifn-gamma) in the same balf was negatively correlated wit ... | 2006 | 16978067 |
| the role of immunostimulation in the development of postweaning multisystemic wasting syndrome in pigs under field conditions. | we evaluated the effects of immunostimulation in the development of postweaning multisystemic wasting syndrome (pmws) in 930 pigs 53 to 54 d old in a grower/finisher barn with a history of pmws. the pigs were allocated to 5 treatment groups: 4 groups received a single intramuscular injection of respisure-one (a commercial mycoplasma hyopneumoniae vaccine; n = 197), emulsigen (an oil-based adjuvant; n = 172), alhydrogel (an aluminum-hydroxide-based adjuvant; n = 172), or physiologic saline (n = 2 ... | 2006 | 17042379 |
| the impact of animal age, bacterial coinfection, and isolate pathogenicity on the shedding of porcine reproductive and respiratory syndrome virus in aerosols from experimentally infected pigs. | the objective of this study was to evaluate the role of different variables (animal age, bacterial coinfection, and isolate pathogenicity) on the shedding of porcine reproductive and respiratory syndrome virus (prrsv) in aerosols. animals were grouped according to age (2 versus 6 mo) and inoculated with a prrsv isolate of either low (mn-30100) or high (mn-184) pathogenicity. selected animals in each group were also inoculated with mycoplasma hyopneumoniae. the pigs were anesthetized and aerosol ... | 2006 | 17042383 |
| isolation of mycoplasma hyopneumoniae from different sampling sites in experimentally infected and contact spf piglets. | the purpose of this study was to determine the optimal route of infection and the optimal sampling sites for the recovery of m. hyopneumoniae, the etiological agent of enzootic porcine pneumonia. virulence of two strains, bq 14 and 116, isolated in france in 1975 and 2003, respectively, was also compared. groups of specific pathogen free piglets were experimentally infected by the intratracheal or intranasal route. one non-inoculated pig was placed in each group of infected pigs to study direct ... | 2007 | 17116374 |
| porcine acute phase protein concentrations in different diseases in field conditions. | five acute phase proteins (apps) [c-reactive protein (crp), serum amyloid a (saa), haptoglobin (hp), pig-map and albumin] were measured in pigs with naturally occurring infections by porcine reproductive and respiratory syndrome virus (prrsv), aujeszky's disease virus (adv), porcine circovirus type 2 (pcv2) and mycoplasma hyopneumoniae, and in animals with tail and ear bites, arthritis and other acute inflammatory processes. healthy specific pathogen-free (spf) pigs were used as controls. in prr ... | 2006 | 17123428 |
| interactions of highly and low virulent mycoplasma hyopneumoniae isolates with the respiratory tract of pigs. | mycoplasma hyopneumoniae is the etiological agent of swine enzootic pneumonia, a chronic nonfatal disease affecting pigs of all ages. to obtain better insight in the mechanisms responsible for differences in virulence between highly and low virulent m. hyopneumoniae isolates, 23 caesarean-derived, colostrum-deprived piglets were randomly assigned to three groups. groups 1 and 2 consisted of nine animals each, which were intratracheally inoculated at 1 week of age with a highly or a low virulent ... | 2007 | 17123752 |
| proteomic survey of the pathogenic mycoplasma hyopneumoniae strain 7448 and identification of novel post-translationally modified and antigenic proteins. | mycoplasma hyopneumoniae is an important pathogen for pigs, being the causative agent of enzootic pneumonia. recently, the genome sequences of three strains, j, 7448 and 232 have been reported. here, we describe the results of a proteomic analysis, based on two-dimensional gel electrophoresis of soluble protein extracts, immunoblot and mass spectrometry, which was carried out aiming the identification of gene products and antigenic proteins from the m. hyopneumoniae pathogenic strain 7448. a pre ... | 2007 | 17182197 |
| protein variability among mycoplasma hyopneumoniae isolates. | sodium-dodecyl-sulphate polyacrylamide gel electrophoresis (sds-page) was used to study the protein variability of mycoplasma hyopneumoniae isolates. fifty-six m. hyopneumoniae isolates from 6 different countries and 37 different herds were used. from eight herds, more than one isolate was available. all sds-page patterns of isolates originating from different herds were clearly divergent. intra-species protein variability was quantified using the reference strain j and seven field strains all o ... | 2007 | 17194551 |
| epidemiologic assessment of porcine circovirus type 2 coinfection with other pathogens in swine. | to identify important pathogens and characterize their serologic and pathologic effects in porcine circovirus type 2 (pcv2)-infected pigs in relation to pig age and type of swine production system. | 2007 | 17223759 |
| [possibilities and limits of serological diagnosis in pig stocks in the case of mycoplasma hyopneumoniae infection]. | when conducting their investigations to diagnose infectious diseases in swine, practitioners are often forced to use reduced numbers of animals in their samples in order to minimize costs for farmers. a cross-sectional study was conducted approximating such field conditions to show the limits of interpretation with reduced sample sizes in case of enzootic pneumonia. compared with other respiratory pathogens, mycoplasma hyopneumoniae, the etiologic agent of enzootic pneumonia spreads very slowly, ... | 2006 | 17233280 |
| immunohistochemical detection of interleukin-12 and interferon-gamma in pigs experimentally infected with mycoplasma hyopneumoniae. | the expression of interleukin-12 (il-12) and interferon-gamma (ifn-gamma) was examined immunohistochemically in the lungs of pigs aged 21 days infected experimentally with mycoplasma hyopneumoniae (mh). ten pigs were inoculated intranasally with mh and killed in pairs weekly from 7 to 35 days post-infection (dpi). immunolabelling for il-12 and ifn-gamma was usually associated with inflammation, particularly in macrophages and lymphocytes in the thickened alveolar septa and in the hyperplastic br ... | 2007 | 17258224 |
| exploratory field study on mycoplasma hyopneumoniae infection in suckling pigs. | the present study focused on mycoplasma hyopneumoniae (m. hyopneumoniae) detection by npcr in nasal swabs of 507 suckling pigs. these animals came from 69 sows (from 1 to 8 parity number) of a farrow-to-finish herd with enzootic pneumonia (ep) problems at finishing stages. at 1 and 3 weeks of age (still in the farrowing units), nasal swabs and blood samples were taken from all piglets. moreover, from these 507 animals, 37 randomly selected pigs were necropsied at 3 weeks of age. from those necro ... | 2007 | 17276021 |
| chronological study of mycoplasma hyopneumoniae infection, seroconversion and associated lung lesions in vaccinated and non-vaccinated pigs. | a field trial was conducted to study mycoplasma hyopneumoniae (mh) infection dynamics by nested polymerase chain reaction (npcr) and serology in pigs of a farm affected by enzootic pneumonia (ep). moreover, correlation of mh detection at different respiratory tract sites with presence of ep gross and microscopic lung lesions was assessed. these parameters were studied and compared between vaccinated (two doses at 1 and 3 weeks of age versus one dose at 6 weeks of age) and non-vaccinated pigs. an ... | 2007 | 17303351 |
| pneumonia disease assessment using a slaughterhouse lung-scoring method. | the aims of this study were the evaluation of a quantitative method for the assessment of pneumonia lesions applied to heavy-weight slaughtered pigs, the identification of risk factors connected with the increase in the prevalence and severity of the lesions and the evaluation of a possible correlation between the presence of pneumonia lesions and the decrease in the carcass quality. the lungs of 10 041 pigs (109 slaughtered batches) coming from 91 farms located in northern italy were examined. ... | 2007 | 17305969 |
| mycoplasma hyopneumoniae mhp379 is a ca2+-dependent, sugar-nonspecific exonuclease exposed on the cell surface. | mycoplasma hyopneumoniae mhp379 is a putative lipoprotein that shares significant amino acid sequence similarity with a family of bacterial thermostable nucleases. to examine the nuclease activity of mhp379, the gene was cloned and expressed in escherichia coli following the deletion of the amino-terminal signal sequence and prokaryotic lipoprotein cleavage site and mutagenesis of the mycoplasma tga tryptophan codons to tgg. the recombinant fusion protein yielded a 33-kda thrombin cleavage produ ... | 2007 | 17307846 |
| efficacy of an inactivated aqueous vaccine for the control of enzootic pneumonia in pigs infected with mycoplasma hyopneumoniae. | the efficacy of an inactivated aqueous vaccine against mycoplasma hyopneumoniae was evaluated at two m hyopneumoniae-infected farrow-to-finish commercial farms (a and b) in greece. in a prospective, randomised double-blind study, two groups on each farm received intramuscular doses of either the vaccine or the adjuvant when they were one and four weeks of age. the pigs were observed daily for clinical signs of disease; morbidity and mortality were recorded; and bodyweight was recorded at interva ... | 2007 | 17308019 |
| detection of respiratory pathogens in porcine lung tissue and lavage fluid. | the objective of this study was to compare the detection rate of bacterial agents in bronchoalveolar lavage fluid (balf), taken without visual control, to that in affected lung tissue obtained from the same pig at necropsy. balf and affected lung tissue were examined for mycoplasma hyopneumoniae using pcr, and standard cultural methods were used for actinobacillus pleuropneumoniae, bordetella bronchiseptica, haemophilus parasuis, pasteurella multocida and streptococcus suis. all pigs with a hist ... | 2008 | 17339121 |
| influence of rearing conditions and respiratory disease on haptoglobin levels in the pig at slaughter. | associations between serum concentrations of haptoglobin, pathological lung lesions indicative of mycoplasma hyopneumoniae (ep) or actinobacillus pleuropneumoniae (pl) infection at slaughter and previous rearing environment were investigated in 510 pigs (90-100 kg live weight) from 17 farms in england. haptoglobin concentrations were significantly higher in pigs showing pathological signs of ep infection compared to those without signs of this disease (ep positive median 0.43 mg ml(-1) vs. ep ne ... | 2007 | 17360012 |
| application of flow cytometry for the determination of minimal inhibitory concentration of several antibacterial agents on mycoplasma hyopneumoniae. | in this study, flow cytometry was evaluated for the determination of the minimal inhibitory concentrations (mics) of nine antibacterial agents (enrofloxacin, ciprofloxacin, oxytetracycline, chloramphenicol, tylosin, lincomycin, gentamycin, spectinomycin and streptomycin) against m. hyopneumoniae. | 2007 | 17381757 |
| pasteurella multocida and its role in porcine pneumonia. | pasteurella multocida has been recognized as a contributor to debilitating and fatal porcine pneumonia for at least 120 years and there continues to be sustained, unabated high prevalence of the organism in cases submitted for diagnostic work up. understanding of its role in disease has been limited, in part because of difficulty in reproducing the disease experimentally with capsular type a strains of p. multocida, the predominant type associated with porcine pneumonia. this limitation has stym ... | 2006 | 17389051 |
| immune responses induced by replication-defective adenovirus expressing the c-terminal portion of the mycoplasma hyopneumoniae p97 adhesin. | mycoplasma hyopneumoniae, the causative agent of porcine enzootic pneumonia, colonizes the respiratory cilia of affected swine, causing significant economic losses to swine production worldwide. vaccination is the most cost-effective strategy for the control and prevention of this disease. the goal of this study was to design and evaluate a replication-defective recombinant adenovirus, radp97c, expressing the c-terminal portion of p97 adhesin (p97c), an important pathogenesis-associated protein ... | 2007 | 17409219 |
| enzootic pneumonia in feeder pigs: observations on causal factors. | a number of factors were studied in eight feeder pig herds, affected with severe or mild enzootic pneumonia, in order to identify those associated with this disease. piggeries with poor facilities and management and where procurement of piglets was from sales barns were more severely affected with enzootic pneumonia than were those with good facilities and where pigs originated directly from breeding units. serological tests and virus isolation revealed that all herds had been exposed to mycopla ... | 1989 | 17423261 |
| enzootic pneumonia in feeder pigs: association with transmissible gastroenteritis virus infection. | infection with transmissible gastroenteritis virus (tgev) was present in some pigs on arrival at a feeder pig farm and was well established three weeks later. tge infection preceded mycoplasma hyopneumoniae infection, which was not detected until three weeks after arrival. severe lesions of enzootic pneumonia were observed at the end of the fattening period.a trial was subsequently done in six-week-old-pigs in order to evaluate the potentiating effect of tgev infection on experimental m. hyopneu ... | 1989 | 17423290 |
| application and field validation of a pcr assay for the detection of mycoplasma hyopneumoniae from swine lung tissue samples. | a pcr assay was validated for the detection of mycoplasma hyopneumoniae in porcine lung tissue. the detection limit of the assay was 0.18 colony-forming units/g of lung sample spiked with m. hyopneumoniae. in field validation, 426 pigs from 220 cases were examined for m. hyopneumoniae infection by m. hyopneumoniae pcr and a fluorescent antibody (fa) test. in total, 103 pig lungs (24.2%) were positive in the pcr test, and 69 pig lungs (16.2%) were positive in the fa test, among which, 62 pigs wer ... | 2007 | 17459839 |
| diversity of mycoplasma hyopneumoniae in pig farms revealed by direct molecular typing of clinical material. | mycoplasma hyopneumoniae is the etiological agent of enzootic pneumonia in swine. various reports indicate that different strains are circulating in the swine population. we investigated the variety of m. hyopneumoniae strains by a newly developed genetic typing method based on the polyserine repeat motif of the lpps homolog p146. pcr amplification using m. hyopneumoniae specific, conserved primers flanking the region encoding the repeat motif, followed by sequencing and cluster analysis was car ... | 2007 | 17506969 |
| [monitoring of infectious diseases among wild boars]. | sixty-seven serum samples and 43 pathological material samples from wild boars, taken in 5 regions of russia and in the kharkov region of the ukraine in 2002 to 2005 were studied. wild boars in some regions of russia were shown to be carriers of aujeszky's disease virus, porcine parvovirus, porcine circovirus type 2, lymphotropic herpesvirus-1, porcine cytomegalovirus, mycoplasma hyopneumoniae and pasteurella multocida. the classical swine fever (csf) virus genome was detected by polymerase chai ... | 2007 | 17601049 |
| effect of mycoplasma hyopneumoniae colonization at weaning on disease severity in growing pigs. | to determine whether mycoplasma hyopneumoniae colonization at weaning in off-site weaning systems is associated with the severity of respiratory disease due to this agent in growing pigs, we studied 20 groups, each group representing a different week in production, in sow herds at 3 farms of 3000 sows each that had a prevalence of m. hyopneumoniae colonization at weaning higher than 5%. the calculated sample size for assessment at weaning was 39 piglets for each group under study; 39 litters wer ... | 2007 | 17695594 |
| mycoplasma hyopneumoniae bacterins and porcine circovirus type 2 (pcv2) infection: induction of postweaning multisystemic wasting syndrome (pmws) in the gnotobiotic swine model of pcv2-associated disease. | groups (5 to 15 per group) of gnotobiotic swine were infected oronasally with porcine circovirus type 2 (pcv2) at 3 days of age and then given 1 of 6 different commercial mycoplasma hyopneumoniae (m. hyopneumoniae) bacterins as either a single dose (7 d of age, 1 application products) or 2 doses (7 and 21 d of age, 2 application product). control groups received pcv2 alone (n = 9) or were infected with pcv2 and immunized twice with keyhole limpet hemocyanin (klh) emulsified in incomplete freund' ... | 2007 | 17824156 |
| effect of sow vaccination against mycoplasma hyopneumoniae on sow and piglet colonization and seroconversion, and pig lung lesions at slaughter. | the objectives of the present study were to compare mycoplasma hyopneumoniae (mh) colonization and serologic status on mh vaccinated and non-vaccinated sows and to assess the effect of sow vaccination on colonization and serologic status of their piglets at weaning as well as presence of enzootic pneumonia (ep) lung lesions at slaughter. fifty sows (25 vaccinated and 25 unvaccinated) as well as five of their piglets were included in the study. blood samples and nasal swabs from sows at 7 weeks p ... | 2008 | 17826008 |
| array-based genomic comparative hybridization analysis of field strains of mycoplasma hyopneumoniae. | mycoplasma hyopneumoniae is the causative agent of porcine enzootic pneumonia and a major factor in the porcine respiratory disease complex. a clear understanding of the mechanisms of pathogenesis does not exist, although it is clear that m. hyopneumoniae adheres to porcine ciliated epithelium by action of a protein called p97. previous studies have shown variation in the gene encoding the p97 cilium adhesin in different strains of m. hyopneumoniae, but the extent of genetic variation among fiel ... | 2007 | 17873054 |
| comparison of three common methods of lung lavage in healthy pigs. | bronchoscopic, endotracheal and transtracheal lung lavage were evaluated in 38 healthy pigs taken from a nucleus herd in a good state of health with respect to their applicability in practice and the traceability of bacteria, cellular parameters and the antimicrobial peptide pr-39 in the respective lavage fluid samples. the total cell count, qualitative morphological cellular characteristics as well as pr-39 could be determined in all lavage fluid samples, while quantitative cell differentiation ... | 2007 | 17877585 |
| multilocus sequence typing (mlst) of mycoplasma hyopneumoniae: a diverse pathogen with limited clonality. | a multilocus sequence typing (mlst) scheme was established and evaluated for mycoplasma hyopneumoniae, the etiologic agent of enzootic pneumonia in swine with the aim of defining strains. putative target genes were selected by genome sequence comparisons. out of 12 housekeeping genes chosen and experimentally validated, the 7 genes efp, metg, pgib, reca, adk, rpob, and tpia were finally used to establish the mlst scheme. their usefulness was assessed individually and in combination using a set o ... | 2008 | 17884308 |
| [transmission of agents of the porcine respiratory disease complex (prdc) between swine herds: a review. part 1--diagnosis, transmission by animal contact]. | knowledge on the different ways of transmitting prdc pathogens (prrsv, influenza virus a, pcv 2, m. hyopneumoniae, a. pleuropneumoniae) between swine herds is of special interest for the development of biosecurity measures or the retrospective risk analysis in the framework of activities of the consulting veterinarian. in this literature review the current knowledge of the transmission of prdc-pathogens is summarized. since the assessment of investigations into pathogen detection in detail is in ... | 2007 | 17927073 |
| surfactin c inhibits mycoplasma hyopneumoniae-induced transcription of proinflammatory cytokines and nitric oxide production in murine raw 264.7 cells. | intact pathogenic mycoplasma hyopneumoniae at 100 microg protein ml(-1) induced transcription of proinflammatory cytokines such as cyclooxygenase (cox)-2, tumor necrosis factor (tnf)-alpha, interleukin(il)-1, il-6 and inducible nitric oxide synthase (inos) in raw 264.7 cells. after pretreatment with 50 microg surfactin c/ml, purified from bacillus subtilis, transcription of the cox-2, il-1beta, il-6 and inos genes induced by m. hyopneumoniae was inhibited by 43%, 82%, 72% and 59%, respectively. | 2008 | 17928958 |
| infection dynamics of porcine reproductive and respiratory syndrome virus in a continuous-flow population of pigs also infected with mycoplasma hyopneumoniae. | twenty-eight 10-week-old pigs were inoculated intratracheally with 1 x 10(5) colour-changing units/ml mycoplasma hyopneumoniae strain 232, and another 32 pigs were not inoculated but were divided into 12 direct-contact pigs and 20 indirect-contact pigs. thirty-five days later, the inoculated pigs were inoculated intranasally with 1 x 10(2.4) tcid50 of porcine reproductive and respiratory syndrome virus (prrsv) strain mn 30-100. viraemia, seroconversion and the transmission of prrsv in the m hyop ... | 2007 | 17938409 |
| resistance mechanism against fluoroquinolones in mycoplasma hyopneumoniae field isolates. | the quinolone resistance-determining regions (qrdr) of gyra, gyrb, parc, and pare of ten mycoplasma hyopneumoniae field isolates that were either sensitive (5) or resistant (5) to the fluoroquinolones flumequine and enrofloxacin were characterized. in all five resistant isolates, one point mutation (c --> a) in parc was found, resulting in an amino acid change from serine to tyrosine at position 80 (escherichia coli numbering). for four of these isolates, this was the only mutation found. these ... | 2007 | 17949302 |
| in vitro expression of the 50-kda and 30-kda fragments of the p97 adhesin of mycoplasma hyopneumoniae in escherichia coli and their use for serodiagnosis. | this article reports the cloning and expression of 2 fragments of the p97 adhesin of mycoplasma hyopneumoniae for use in serodiagnosis: a 50-kda fragment (including the n-terminal cleavage site) and a 30-kda fragment (including the c-terminal r1 and r2 repeats, which are essential for adherence). the genes encoding the fragments were amplified, cloned, and expressed in the escherichia coli expression system bl21 (de3)plyss. antiserum against the purified recombinant proteins reacted with the myc ... | 2007 | 17955902 |
| control of mycoplasma hyopneumoniae infections in pigs. | mycoplasma hyopneumoniae, the primary pathogen of enzootic pneumonia, occurs worldwide and causes major economic losses to the pig industry. the organism adheres to and damages the ciliated epithelium of the respiratory tract. affected pigs show chronic coughing, are more susceptible to other respiratory infections and have a reduced performance. control of the disease can be accomplished in a number of ways. first, management practices and housing conditions in the herd should be optimized. the ... | 2008 | 17964089 |
| [transmission of agents of the porcine respiratory disease complex (prdc) between swine herds: a review. part 2--pathogen transmission via semen, air and living/nonliving vectors]. | the transmission of prdc-pathogens (prrsv, influenza virus a, pcv2, m. hyopneumoniae, a. pleuropneumoniae) between swine herds, which was summarized in the first part of the review, mainly occurs via pig movement. the risk of pathogen transmission by insemination with contaminated semen plays only a relevant role in the infection with prrsv and pcv2. a risk of the aerogen transmission of pathogens between herds within a distance of 2 to 3 km is described for m. hyopneumoniae and prrsv. evidence ... | 2007 | 17970334 |
| global transcriptional analysis of mycoplasma hyopneumoniae following exposure to hydrogen peroxide. | mycoplasma hyopneumoniae, the causative agent of swine enzootic pneumonia, colonizes the cilia of swine lungs, causing ciliostasis and cell death. m. hyopneumoniae is a component of the porcine respiratory disease complex (prdc) and is especially problematic for the finishing swine industry, causing the loss of hundreds of millions of dollars in farm revenues worldwide. for successful infection, m. hyopneumoniae must effectively resist oxidative stresses due to the release of oxidative compounds ... | 2007 | 17975087 |
| the relationship between infectious and non-infectious herd factors with pneumonia at slaughter and productive parameters in fattening pigs. | this paper explores the relationship between infectious and non-infectious herd factors with the occurrence of pneumonia at slaughter and productive parameters in fattening pigs on 39 fattening herds. a questionnaire was used to obtain environmental and management factors (non-infectious factors). blood samples and lungs were obtained from 35 pigs in each herd at slaughter. serological testing was performed for antibodies against three respiratory pathogens (infectious factors): porcine reproduc ... | 2009 | 18023215 |
| transcriptome changes in mycoplasma hyopneumoniae during infection. | mycoplasma hyopneumoniae causes swine pneumonia and contributes significantly to the porcine respiratory disease complex. the mechanisms of pathogenesis are difficult to address, since there is a lack of genetic tools, but microarrays are available and can be used to study transcriptional changes that occur during disease as a way to identify important virulence-related genes. mycoplasmas were collected from bronchial alveolar lavage samples and compared to broth-grown cells using microarrays. b ... | 2008 | 18070898 |
| mycoplasmas regulate the expression of heat-shock protein genes through circe-hrca interactions. | mycoplasmas in general are rarely exposed to severe environmental changes except during its colonization and infection processes. genomic analysis indicates that mycoplasma hyopneumoniae possesses the genes of a single sigma factor and the hrca repressor of negative regulation of the heat-shock response. a perfect inverted repeat sequence (5'-ctggcactt-n(9)-aagtgccaa-3') upstream of the dnak gene has also been identified. in the present study, we demonstrate the functionality of hrca-circe inter ... | 2008 | 18164681 |
| passive transfer of maternal mycoplasma hyopneumoniae-specific cellular immunity to piglets. | immunity in the neonatal animal is primarily maternally derived, either by lymphocytes that pass into the newborn across the placenta or following colostrum ingestion. however, the effect of this passively transferred cellular maternal immunity on the newborn's immune repertoire is not clearly understood. various studies have shown that colostral lymphocytes are activated and possess functional abilities; however, no studies have shown the transfer of colostral antigen-specific t-cell-specific r ... | 2008 | 18184823 |
| susceptibility testing of tulathromycin: interpretative breakpoints and susceptibility of field isolates. | in vitro susceptibility tests were conducted on bovine and porcine respiratory pathogens isolated from european countries during 2004-2006 for susceptibility to tulathromycin using the recommended methodologies for broth microdilution. the results were compared with data from a similar survey conducted prior to launch in 1998-2001 to monitor for any shift in susceptibility. the importance of maintaining the ph of the culture media within the range 7.2-7.4 was re-affirmed as a key factor in obtai ... | 2008 | 18187275 |
| transmission of pathogenic respiratory bacteria to specific pathogen free pigs at slaughter. | the purpose of this study was to evaluate the transmission of pathogenic respiratory bacteria to thirteen 5-month-old specific pathogen free (spf) pigs, during the slaughtering process in a commercial slaughterhouse. before transportation, the spf pigs and the lorry were checked to confirm the absence of pathogenic respiratory bacteria. nine spf pigs (group 1) were in contact in a conventional slaughterhouse with finishing pigs, during 4h before slaughtering. four spf pigs (group 2) were slaught ... | 2008 | 18242013 |
| a successful national control programme for enzootic respiratory diseases in pigs in switzerland. | before the start of systematic disease control, respiratory diseases in swine in switzerland caused estimated losses of several million euros per year. in 1993, a national programme to control enzootic respiratory diseases in pigs was proposed, with the aim of reducing the incidence of clinical cases to less than 1%. enzootic pneumonia (ep) caused by mycoplasma hyopneumoniae and clinical cases of pleuropneumonia caused by any serotype of actinobacillus pleuropneumoniae (app) would be targeted, i ... | 2007 | 18293608 |
| associations between pathogens in healthy pigs and pigs with pneumonia. | the aim of this study was to evaluate the associations between different pathogens in the development of pneumonia and bronchopneumonia in pigs. samples of bronchoalveolar lavage fluid from 100 pigs showing no clinical signs and 239 pigs with clinical signs of respiratory disease were examined for mycoplasma hyopneumoniae, mycoplasma hyorhinis, us-type porcine respiratory and reproductive syndrome virus (prrsv), eu-type prrsv, porcine circovirus type 2 (pcv-2), influenza virus type a, alpha-haem ... | 2008 | 18310558 |
| current perspectives on the diagnosis and epidemiology of mycoplasma hyopneumoniae infection. | mycoplasma hyopneumoniae is the principal aetiological agent of enzootic pneumonia (ep), a chronic respiratory disease that affects mainly finishing pigs. although major efforts to control m. hyopneumoniae infection and its detrimental effects have been made, significant economic losses in pig production worldwide due to ep continue. m. hyopneumoniae is typically introduced into pig herds by the purchase of subclinically infected animals or, less frequently, through airborne transmission over sh ... | 2009 | 18396428 |