Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| the chemical composition of the membranes of protoplasts and l-forms of staphylococcus aureus. | membrane fractions were prepared from staphylococcus aureus h and 100 after dissolution of the cell walls by a lytic enzyme from streptomyces griseus. membranes were also prepared from the l-forms derived from the same strains. the membranes were analysed for protein, lipid, carbohydrate and rna contents, and the fatty acid composition of the lipids was determined. a branched-chain saturated c(15) acid was the major component in all samples, and the correspondence between l-forms and parent bact ... | 1968 | 4295332 |
| analysis of taxonomic characteristics of highly active viomycin-producing mutants of streptomyces griseus var. purpureus. | 1972 | 4345459 | |
| studies on proteolytic enzymes (pronase) of streptomyces griseus k-1. 3. purification and proteolytic specificity of alkaline proteinase c from pronase. | 1973 | 4352628 | |
| [formation of a melanoid pigment by streptomyces griseus from synthetic media with tyrosine]. | 1968 | 4386058 | |
| the proteolytic enzymes of the k-1 strain of streptomyces griseus obtained from a commercial preparation (pronase). i. purification of four serine endopeptidases. | 1972 | 4624704 | |
| the proteolytic enzymes of the k-1 strain of streptomyces griseus obtained from a commercial preparation (pronase). ii. the activity of a serine enzyme in 6 m guanidinium chloride. | 1972 | 4624705 | |
| the carboxyl-terminal amino acid sequence of streptomyces griseus protease a. | 1972 | 4625393 | |
| l-asparaginase production by streptomyces griseus. | streptomyces griseus atcc 10137 synthesizes about 1 iu of l-asparaginase/100 ml of a 4% peptone medium. the enzyme has a ph optimum of 8.5 which is comparable to that of the l-asparaginase derived from escherichia coli which has antitumor properties. | 1972 | 4626231 |
| chromogenesis mirabilis in streptomyces griseus. | a number of chromogenic streptomyces, producing diffusible melanoid pigment on complex organic media, fail to form melanin pigment on conventionally used synthetic tyrosine agar. by means of our new melanin formation test, almost all the chromogenic streptomyces can now be detected in chemically defined medium. in contrast to ordinary chromogenic streptomyces, two streptomyces species of the international streptomyces project, s. griseus isp 5236 and s. ornatus isp 5307, produce melanin pigment ... | 1972 | 4629704 |
| insulin-like activity of proteases. i. effect of pronase, a protease preparation from streptomyces griseus, on glucose uptake by mouse diaphragm. | 1972 | 4630109 | |
| studies on bacteriolytic enzymes. ii. purification and some properties of two types of staphylolytic enzymes from streptomyces griseus. | 1972 | 4630110 | |
| a proposed structure for streptomyces griseus protease a. | 1972 | 4632174 | |
| the proteolytic enzymes of the k-1 strain of streptomyces griseus obtained from a commercial preparation (pronase). iv. structure-function studies of the two smallest serine endopeptidases; stabilization by glycerol during reaction with acetic anhydride. | 1973 | 4633674 | |
| an endogenous factor regulating the life cycle of streptomyces griseus. | 1967 | 4878402 | |
| remarkable homology about the disulfide bridges of a trypsin-like enzyme from streptomyces griseus. | 1969 | 4899581 | |
| rna synthesis on native dna complexes isolated from streptomyces griseus and escherichia coli. | 1970 | 4922164 | |
| [extent of the proteolytic action of streptomyces griseus protease on serum lipoproteins]. | 1965 | 4953404 | |
| studies on proteolytic enzymes (pronase) of streptomyces griseus k-1. i. nature and properties of the proteolytic enzyme system. | 1967 | 4968616 | |
| streptomycin-like compound enzymically released from a streptomycin-non-producing streptomyces griseus strain. | 1968 | 4971329 | |
| change of resistance to lysozyme and ultrasonic disintegration of the mycelium of streptomyces griseus under the influence of chelating agents and polyvalent cations. | 1968 | 4971766 | |
| immunofluorescence identification of thermopolyspora polyspora, the causative agent of farmer's lung. | farmer's lung is a serious disabling pulmonary disease found in agricultural workers. the disease is believed to be a hypersensitivity to the thermophilic actinomycetes, principally thermopolyspora polyspora. this organism is difficult to stain with the usual bacteriological stains and thus far has not been demonstrated in the lung tissue by microscopic methods. in this paper, it is demonstrated that the fluorescent-antibody technique is a simple method for the positive identification of t. poly ... | 1969 | 4976327 |
| the purification and properties of two staphylolytic enzymes from streptomyces griseus. | 1. two staphylolytic enzymes have been purified from cultures of a soil isolate of streptomyces griseus. 2. the purified enzymes were shown to be basic proteins of low molecular weight. each enzyme released n-acetylmuramic acid reducing groups from the cell walls of staphylococcus aureus. 3. the enzymes lysed whole staphylococci best at higher ph values and lower ionic strengths than when the substrate was isolated cell walls or purified mucopeptide. 4. added teichoic acid did not inhibit the en ... | 1968 | 4976493 |
| the specificity of proteinases from streptomyces griseus (pronase). | purification of pronase by ion-exchange chromatography gave four proteolytically active fractions. fraction a(2) contained an endopeptidase that attacks poly l-valine. fraction b contained an endopeptidase, an aminopeptidase and carboxypeptidases. the activities against hippuryl-l-arginine and hippuryl-l-phenylalanine could be inhibited to a considerable extent by di-isopropyl phosphorofluoridate and by edta. fraction c contained an endopeptidase resembling bovine trypsin. the pure enzyme was co ... | 1970 | 4983492 |
| location and action of streptomyces griseus alpha-d-mannosidase, an antibiotic-transforming enzyme. | 1970 | 4990331 | |
| antigenicity of proteinases from streptomyces griseus (pronase). | the five pronase fractions, a(1), a(2), b, c (trypsin-like), and d (elastolytic), obtained by ion-exchange chromatography, were found to be antigenically distinct. antibodies to pronase inhibited the enzymic activity of each of the enzyme fractions. pronase trypsin and bovine trypsin, although resembling each other in enzymic activity and in amino acid sequence around their active sites, did not cross-react antigenically with, nor was their enzymic activity inhibited by, the respective homologou ... | 1970 | 4992320 |
| fast sedimenting fractions containing dna from streptomyces griseus. | 1970 | 4992457 | |
| the elastase-like enzymes from streptomyces griseus (pronase). isolation and partial characterization. | 1971 | 4994590 | |
| studies of the heterogeneity of streptomyces griseus protease. i. polyacrylamide gel electrophoresis of commercial pronase-p, derived from streptomyces griseus k1. | 1971 | 4999784 | |
| streptomycin biosynthesis. i. existence of two amidinotransferase activities in streptomyces griseus. | 1971 | 5003716 | |
| the amino acid sequence of streptomyces griseus protease a: the peptic peptides. | 1971 | 5003934 | |
| [reaktion of iron deficiency on metabolism of streptomyces griseus]. | 1971 | 5004553 | |
| [properties of the crystalline complex of proteases from streptomyces griseus]. | 1971 | 5005241 | |
| a new component from the cell wall of streptomyces griseus. i. the role of streptomycin in the life of streptomyces griseus. | 1965 | 5006488 | |
| [comparative study of the composition of streptothricins produced by cultures of actinomyces griseus 15 and its phage-resistant variants]. | 1972 | 5084529 | |
| [comparative study of methods for extracting polysaccharides from actinomyces griseus 15]. | 1971 | 5096619 | |
| [some characteristics of actinophages of the polylysogenic culture of actinomyces griseus 15]. | 1971 | 5130741 | |
| products of the dissociation of lipopolysaccharide by streptomyces griseus extracellular enzyme. | 1965 | 5324812 | |
| changes of nucleic acid content of streptomyces griseus strains in submerged culture. | 1970 | 5425489 | |
| studies on streptomyces griseus protease. iv. formation of 14c-acetyl enzyme of two dfp-sensitive components during reaction with p-nitrophenyl [1-14c] acetate. | 1970 | 5426219 | |
| isolation and absolute configuration of indolmycenic acid, an intermediate in the biosynthesis of indolmycin by streptomyces griseus. | 1970 | 5447649 | |
| accumulation of streptomycin-phosphate in cultures of streptomycin producers grown on a high-phosphate medium. | a phosphorylated derivative of streptomycin accumulated in cultures of streptomyces griseus atcc 12475 and sc2376 grown on complex media containing an excess of inorganic phosphate (0.01 m). this compound did not accumulate significantly in the absence of added inorganic phosphate. the phosphorylated derivative did not inhibit growth of bacillus subtilis or support growth of a streptomycin-dependent strain of escherichia coli; however, incubation of the derivative with alkaline phosphatase gave ... | 1970 | 5473919 |
| demonstration of a c-methylating enzyme in cell free extracts of indolmycin-producing streptomyces griseus. | 1970 | 5490210 | |
| [gelatin hydrolysis by protease of streptomyces griseus]. | 1970 | 5492095 | |
| comparison of deoxyribonucleic acids from streptomyces griseus strains. | 1970 | 5518856 | |
| the disulfide bridge sequences of a serine protease of wide specificity from streptomyces griseus. | 1971 | 5575653 | |
| [method for crystallizing the protease of streptomyces griseus]. | 1967 | 5603082 | |
| application of the lowry's protein determination method for the mycelium of streptomyces griseus strain no. 52-1. | 1967 | 5630774 | |
| studies on streptomyces griseus protease. i. separation of dfp-reacting enzymes and purification of one of the enzymes. | 1968 | 5636371 | |
| studies on streptomyces griseus protease. ii. the amino acid sequence around the reactive serine residue of dfp-sensitive components with esterase activity. | 1968 | 5636372 | |
| a piecemeal description of streptomyces griseus (krainsky) waksman and henrici. | 1968 | 5640225 | |
| nocardia dassonvillei, a macroscopic replica of streptomyces griseus. | 1968 | 5640226 | |
| resistance of alkaline phosphatase of human serum to the protease of streptomyces griseus. | 1968 | 5657464 | |
| the metabolism of scyllo-inositol in streptomyces griseus. | 1968 | 5683534 | |
| the trypsin-like enzyme from streptomyces griseus (pronase). | 1968 | 5685881 | |
| studies on proteolytic enzymes (pronase) of streptomyces griseus k-1. ii. separation of exo- and endopeptidases of pronase. | 1968 | 5707831 | |
| role of streptomycin in the life of streptomyces griseus: streptidine-containing fractions in the cell walls of streptomyces griseus strains. | 1968 | 5710269 | |
| [properties of the proteolytic complex of streptomyces griseus]. | 1968 | 5727732 | |
| cytomorphological effect of factor c in submerged cultures on the hyphae of streptomyces griseus strain no. 52-1. | 1969 | 5792194 | |
| studies on streptomyces griseus protease. 3. purification of two dfp-reacting enzymes. | 1969 | 5796105 | |
| the biosynthesis of streptomycin. the origin of the c-formyl group of streptose. | 1. the biosynthesis of streptomycin in streptomyces griseus has been studied by adding d-[3,4-(14)c(2)]glucose or d-[1,3-(14)c(2)]glucose to the growth medium and degrading the streptomycin produced. 2. the results suggest that the c-3' branch carbon atom of l-streptose arises from c-3 of d-glucose. 3. the mechanism of biosynthesis of streptose from glucose is discussed. it probably involves an intramolecular rearrangement of a 6-deoxy-4-oxyhexose derivative, and it is suggested that the nucleos ... | 1965 | 5837790 |
| the effect of catalase on the redox-changes of streptomyces griseus 134-culture. preliminary communication. | 1965 | 5839600 | |
| reaction of two enzyme fractions from streptomyces griseus protease with diisopropylphosphoro-fluoridate. | 1965 | 5850139 | |
| the metabolism of streptomyces griseus. x. a study of streptomyces griseus cytochrome b. | 1965 | 5865168 | |
| geosmin, an earthly-smelling substance isolated from actinomycetes. | geosmin, an earthy-smelling substance, has been isolated from several actinomycetes. production of 1 mg per liter of whole broth was obtained from streptomyces griseus lp-16. after preliminary separations, pure geosmin was isolated in milligram amounts by gas chromatography. geosmin is a neutral oil, with an approximate boiling point of 270 c, which contains carbon and hydrogen, but no nitrogen. it undergoes a reaction with acid to give odorless argosmin, a neutral oil, with an approximate boili ... | 1965 | 5866039 |
| fermentation studies with streptomyces griseus. i. carbohydrate sources for the production of protease and streptomycin. | the effects of four carbon sources and inorganic phosphate on the production of streptomycin and protease by a strain of streptomyces griseus were studied. protease production was increased in fermentations with comparatively rapid consumption of carbohydrate, and streptomycin was produced under conditions of moderately slow consumption. starch was consumed more rapidly than glucose, and, in fermentations with starch as a carbon source, good yields of protease were associated with poor yields of ... | 1965 | 5867647 |
| [studies on the self-limitation of streptomyces griseus and streptomyces rimosus]. | 1965 | 5877738 | |
| on the hypoglycemic activity of protease preparation of streptomyces griseus, pronase p. | 1964 | 5888652 | |
| [studies on the tissue metabolism in streptomyces griseus and its relation to antibiotic biosynthesis]. | 1965 | 5898718 | |
| inhibitory effect of cysteine on streptomyces griseus phage reproduction. | 1966 | 5918963 | |
| a comparison of the methods of quantitative determination of nucleic acids in streptomyces griseus. | 1966 | 5922462 | |
| binding of homologous polymerized deoxyribonucleic acid by streptomyces griseus. | germaine, g. r. (university of minnesota, minneapolis), and d. l. anderson. binding of homologous polymerized deoxyribonucleic acid by streptomyces griseus. j. bacteriol. 92:662-667. 1966-an irreversible p(32) deoxyribonucleic acid (dna) binding system is described for streptomyces griseus s104. the 69% guanine plus cytosine (gc) s. griseus dna was bound by late exponential -early stationary phase cultures. simultaneous addition of deoxyribonuclease with the p(32)-dna to maximally receptive cult ... | 1966 | 5922540 |
| 3-o-methyl-d-mannose from streptomyces griseus. | 1. streptomyces griseus was grown in a medium containing l-[me-(14)c]methionine, and the labelled products from an ethanolic extract of the cells were examined. 2. acid hydrolysis of one of the products gave a compound identified as 3-o-[me-(14)c]-methylmannose by a series of degradative reactions. 3. reduction of the radioactive compound gave 3-o-methyl-d-mannitol, indistinguishable from a synthetic sample. | 1966 | 5938638 |
| isolation of uridine diphosphate n-acetyl-d-glucosamine from streptomyces griseus. | 1966 | 5962679 | |
| metabolism of progesterone by streptomyces griseus. | 1966 | 5965139 | |
| fermentation studies with streptomyces griseus. ii. synthetic media for the production of streptomycin. | synthetic media for streptomycin fermentation were studied to determine which media gave highest yields of streptomycin. the effect of salts on streptomycin production by streptomyces griseus was examined, and a suitable combination of salts was established in a glucose-casein medium. this medium yielded 3,000 mug/ml of the antibiotic with an inoculum of 1.6%. substitution of amino acids for casein was examined. of 17 amino acids tested, best results were obtaind with sodium aspartate. substitut ... | 1966 | 5970459 |
| [studies on the anti-inflammatory action of digestive enzymes: anti-edematous action of protease from streptomyces griseus and alpha-amylase from bacillus amylosolvens]. | 1966 | 6010714 | |
| the mode of action of an alkaline proteinase from streptomyces griseus on baker's yeast cytochrome c. | 1967 | 6050559 | |
| antibiotic production of hyphal fractions of streptomyces griseus. i. determination of streptomycin producing activity of washed mycelium in short-term incubation. | 1967 | 6051518 | |
| killing and photoreactivation of streptomyces griseus conidia by vacuum-ultraviolet and far-ultraviolet radiation (1500 to 2700 a). | 1967 | 6053866 | |
| isolation of different mycelial fractions from submerged culture of streptomyces griseus strain by density gradient centrifugation. | 1967 | 6072918 | |
| antibiotic production of hyphal fractions of streptomyces griseus. ii. streptomycin production of different fractions obtained by density gradient centrifugation. | the streptomycin-producing activity (spa) of hyphal fractions from washed mycelium of submerged cultures of streptomyces griseus strain 52-1, as obtained by density gradient centrifugation, was investigated. activity of the various fractions differed strongly in intensity. the highest spa was evident in the unfractionated mycelium. a synergistic effect upon spa was found in the interaction of cultures of different ages, and a 55% increase in yield was obtained by mixing the 48- and 72-hr culture ... | 1967 | 6077406 |
| new antibacterial agent (u-24,544) isolated from streptomyces griseus. | antibiotic u-24,544 is a new agent isolated from the culture broth of a streptomycete strain. the antibiotic inhibits a variety of gram-positive and gram-negative bacteria in vitro, but is ineffective in treatment of experimental bacterial infections in mice. it is fairly cytotoxic in mammalian cell cultures and remarkably nontoxic in mice. | 1967 | 6077411 |
| preliminary investigation of the structure of antibiotics produced by highly active mutants of streptomyces griseus nrrl 3851 and streptomyces lactamdurans nrrl 3802. | streptomyces griseus nrrl 3851 and streptomyces lactamdurans nrrl 3802 are well known as producers of cephamycins a, b and c, respectively. antibiotics produced by their highly active mutants: s. griseus g-12 (termed x-12-1 and x-12-2) and s. lactamdurans l-2 (termed x-2) have been isolated and found to belong also to the cephamycin family. their ir spectra revealed beta-lactam absorption at 1750 cm-1 and their 1h nmr spectra demonstrated the presence of protons attached to 7-methoxycephem skele ... | 1984 | 6083707 |
| physiological changes of streptomyces griseus and streptomyces lactamdurans strains associated with an increase in their ability for cephamycin production. | 1984 | 6083714 | |
| a diffusible factor responsible for the determination of cholinergic functions in cultured sympathetic neurons. partial purification and characterization. | a macromolecule has been partially purified which influences the choice of the neurotransmitter synthesized by sympathetic neurons. previous studies showed that culture medium conditioned by incubation on certain types of non-neuronal cells increased [3h]acetylcholine synthesis and accumulation from [3h]choline in primary cultures of neurons dissociated from neonatal rat superior cervical ganglion and grown in the virtual absence of non-neuronal cells. a concomitant decrease of [3h]catecholamine ... | 1981 | 6110668 |
| sporulation of streptomyces griseus in submerged culture. | a wild-type strain of streptomyces griseus forms spores both on solid media (aerial spores) and in liquid culture (submerged spores). both spore types are highly resistant to sonication, but only aerial spores are resistant to lysozyme digestion. electron micrographs suggest that lysozyme sensitivity may result from the thinner walls of the submerged spores. studies of the life cycle indicate that neither streptomycin excretion nor extracellular protease activity is required for sporulation: the ... | 1983 | 6134716 |
| susceptibility of protein synthesis to streptomycin in streptomycin-producing streptomyces griseus. | 1980 | 6154597 | |
| biosynthesis of streptomycin. enzymatic formation of dihydrostreptomycin 6-phosphate from dihydrostreptosyl streptidine 6-phosphate. | incubation of o-alpha-l-dihydrostreptose (1 leads to 4) streptidine 6-phosphate (i) with a protein-free extract from streptomyces griseus as endogenous donor and a cell-free extract from this organism led to formation of dihydrostreptomycin 6-phosphate (ii). the product was identified by paper chromatography and by its degradation to dihydrostreptobiosamine (iii). ii was not formed when either the donor solution or the dialysed cell-free extract was omitted. the results corroborate the role of i ... | 1980 | 6157663 |
| 3' terminal labelling of rna of rna with beta-32p-pyrophosphate group and its application to the sequence analysis of 5s rna from streptomyces griseus. | nucleotide pyrophosphate transferase isolated from streptomyces griseus is used to transfer pyrophosphate group from gamma-32p-atp to the 3'-oh of trna, generating a strictly terminal label at its 3' end. using yeast trnaphe as model compound, it is demonstrated that the labelled molecule is suitable for rapid gel sequencing by both enzymatic and chemical methods. rna molecules terminated by pyrimidine nucleoside are poor pyrophosphate acceptors. to label rnas of this kind, first guanosine 5'-ph ... | 1980 | 6159585 |
| the use of pronase enhances sensitivity of the pap method in the detection of intracytoplasmic immunoglobulins. | application of immunocytochemistry to formalin-fixed, paraffin-embedded material tends to provide capricious results, even when employing the unlabelled antibody peroxidase-antiperoxidase (pap) method. this study shows that treatment of tissue sections with pronase, a protease isolated from a strain of streptomyces griseus, prior to performance of the pap procedure greatly enhances its sensitivity. the optimum conditions for use of this enzyme in detecting intracellular immunoglobulins are given ... | 1980 | 6160845 |
| affinophoresis of trypsins. | we devised a new separation technique for the protein, "affinophoresis," which is based on its specific affinity and utilizes electrophoresis. this technique requires a carrier macromolecule, "affinophore," which contains both an affinity ligand for a certain protein and many charges, either positive or negative, in order to migrate rapidly in an electric field. when a mixture of proteins is electrophoresed in the presence of the affinophore, the protein having an affinity with the ligand will f ... | 1982 | 6185472 |
| a method for estimation of the antibiotic activity of streptomyces griseus and streptomyces lactamdurans strains producing cephamycins. | 1983 | 6202109 | |
| fluorometric determination of dna in cartilage of various species. | a sensitive, modified 3,5-diaminobenzoic acid (daba), fluorometric dna assay was developed and compared to mithramycin and ethidium bromide assays in determining the dna content of dense connective tissues including: swarm rat chondrosarcoma, rabbit, dog, monkey, and most importantly, adult human articular cartilage. in the more cellular cartilages, the three methods gave equivalent results. however, in the relatively acellular human cartilage, the daba method was shown to be superior. both the ... | 1984 | 6238138 |
| [biosynthesis of the antibiotic, grizin, by prototrophic revertants of streptomyces griseus biochemical mutants]. | spontaneous and nitrosomethylbiuret-induced prototrophic revertants of various biochemical mutants of str. griseus producing grisin, a streptothricin antibiotic, were isolated. the antibiotic production level of the revertants was studied. it was found that most of the prototrophic revertants synthesized much higher amounts of grisin than the initial biochemical mutants. it was also shown that a number of the prototrophic revertants of the methionine- and arginine-dependent mutants synthesized 2 ... | 1980 | 6249189 |
| protection of actin against proteolysis by complex formation with deoxyribonuclease i. | g-actin bound to deoxyribonuclease i (dnase i) is resistant to digestion by trypsin and chymotrypsin. in the absence of dnase i, g-actin is cleaved by these proteases to yield a 33 500 molecular weight core protein which is not degraded further. the major sites of proteolytic action in the amino acid sequence of actin have been identified as being adjacent to residues arginine-62 and lysine-68 for trypsin and leucine-57 for chymotrypsin. these residues are rendered inaccessible to proteases in t ... | 1980 | 6265044 |
| induction of streptomycin-inactivating enzyme by a-factor in streptomyces griseus. | the effect of a-factor on streptomycin resistance and productivity in streptomyces griseus and s. bikiniensis was studied using a-factor-negative mutants. resistance of several of these mutants was markedly increased by adding a-factor to the growing medium, as also was their streptomycin productivity. the a-factor induced resistance was due to inactivation by streptomycin-6-phosphotransferase, and enzyme synthesis in these mutants was completely dependent on the presence of a-factor. in the cas ... | 1982 | 6292150 |
| cloning of a pleiotropic gene that positively controls biosynthesis of a-factor, actinorhodin, and prodigiosin in streptomyces coelicolor a3(2) and streptomyces lividans. | a-factor (2s-isocapryloyl-3s-hydroxymethyl-gamma-butyrolactone), an autoregulating factor originally found in streptomyces griseus, is involved in streptomycin biosynthesis and cell differentiation in this organism. a-factor production is widely distributed among actinomycetes, including streptomyces coelicolor a3(2) and streptomyces lividans. a chromosomal pleiotropic regulatory gene of s. coelicolor a3(2) controlling biosynthesis of a-factor and red pigments was cloned with a spontaneous a-fac ... | 1983 | 6309746 |
| purification and characterization of streptomycin 6-kinase, an enzyme implicated in self-protection of a streptomycin-producing micro-organism. | streptomycin 6-kinase of the streptomycin-producing strain streptomyces griseus hut 6037 was purified by fractionation with (nh4)2so4 and chromatography on deae-sephadex a-25, hydroxyapatite and sephadex g-100. after page of the final fraction, a protein band corresponding to streptomycin 6-kinase was detected, together with a less intense band having no enzyme activity. molecular weights determined by sds-page and by sephadex g-100 chromatography were about 36000 and 38000, respectively, sugges ... | 1983 | 6313852 |
| partial characterization of thymocyte-activating factor derived from mdp-stimulated guinea pig fibroblasts. | the activity of fibroblast-derived thymocyte activating factor (ftaf) of the guinea pig was measured, and the factor was partially characterized. the ftaf activity was heat labile, and destroyed by treatment with trypsin, chymotrypsin, and streptomyces griseus protease, suggesting the protein nature of ftaf. ftaf bound to deae-sepharose cl-6b in tris-hcl buffer at ph 8.0, and was eluted with 0.1-0.2 m nacl. ftaf was absorbed with blue sepharose cl-6b. the factor bound to a hydroxylapatite column ... | 1984 | 6335219 |
| a triglobular model for the polypeptide chain of aspartokinase i-homoserine dehydrogenase i of escherichia coli. | limited proteolysis of aspartokinase i-homoserine dehydrogenase i from escherichia coli by type vi protease from streptomyces griseus yields five proteolytic fragments, three of which are dimeric, the other two being monomeric. one of the monomeric fragments (27 kilodaltons) exhibits residual aspartokinase activity, while the second one (33 kilodaltons) possesses residual homoserine dehydrogenase activity. the smallest of the dimeric species (2 x 25 kilodaltons) is inactive; the two other dimers ... | 1983 | 6338915 |