Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| conversion of 5' xanthylic acid to guanine and guanine nucleotides by a mutant of brevibacterium ammoniagenes. | 1971 | 5580682 | |
| [synthesis of adenine and guanine nucleotides in brevibacterium ammoniagenes atcc no. 6872]. | 1971 | 5580980 | |
| correlation between spectral changes and synergistic inhibition of bacterial phosphopyruvate hydratase in the presence of two metal ions. | 1967 | 5586498 | |
| [the action of siderochromes on the glutamic acid-forming bacterium brevibacterium flavum atcc 14067]. | 1967 | 5591327 | |
| effect of gamma irradiation on the microflora of freshwater fish. 3. spoilage patterns and extension of refrigerated storage life of yellow perch fillets irradiated to 0.1 and 0.2 megarad. | maximal shelf life was determined and microbial flora were compared for irradiated (0.1 and 0.2 mrad) and nonirradiated yellow perch fillets stored at 1 c. shelf life was estimated by organoleptic determinations. microbiological studies included determination of the effects of irradiation on the total aerobic microbial population, lag phase, and rate of growth. genera of organisms isolated from fillets through the course of microbial spoilage were identified, and the proteolytic activity of the ... | 1968 | 5645411 |
| a quantitative evaluation of the mutagenic effect of ethylmethanesulfonate in brevibacterium ammoniagenes and qualitative character of the mutants obtained. | 1968 | 5658988 | |
| production of nucleic acid-related substances by fermentative processes. xix. accumulation of 5'-inosinic acid by a mutant of brevibacterium ammoniagenes. | the accumulation of 5'-inosinic acid (imp) by a mutant, ky 13102, induced from brevibacterium ammoniagenes atcc 6872 by ultraviolet light irradiation, was examined. although growth was stimulated by adenine or adenosine, the microorganism showed fair growth in the medium containing amino acids but no adenine. among six kinds of natural nutrients tested, meat extract and casamino acids were suitable for the accumulation of imp. manganese ion strongly affected growth, the accumulation of imp and h ... | 1968 | 5664127 |
| formation of o-ethylhomoserine by bacteria. | resting cells of corynebacterium sp. e17 formed o-ethylhomoserine from ethyl alcohol for a few hours. addition of l-homoserine greatly enhanced its formation. thus, the formation of o-ethylhomoserine from ethyl alcohol by 27 bacteria, 6 yeasts, and 4 fungi was investigated by using growing cultures and resting cells in the presence of l-homoserine. the o-ethylhomoserine formed in the culture supernatant fluids or supernatant fluids of the reaction mixtures was identified by paper chromatography. ... | 1968 | 5674048 |
| [synthesis of 7,8-disubstituted, pelargonic acid and its activity]. | 1968 | 5692489 | |
| regulation of the tca and glyoxylate cycles in brevibacterium flavum. i. ingibition of isocitrate lyase and isocitrate dehydrogenase by organic acids related to the tca and glyoxylate cycles. | 1968 | 5707822 | |
| unbalanced growth death due to depletion of mn2+ in brevibacterium ammoniagenes. | in the microbial conversion of added hypoxanthine to 5'-inosinic acid, mn(2+) concentration in the growth medium is known to have a profound effect both on the yield of 5'-inosinic acid and the morphology of cells of brevibacterium ammoniagenes. to elucidate the mechanism in which mn(2+) was concerned with cell morphology and 5'-inosinic acid production, effects of mn(2+) on the macromolecular synthesis were measured. it was found that mn(2+) strongly governed deoxyribonucleic acid (dna) synthes ... | 1968 | 5726310 |
| [microbiological method of synthesis of p32-labelled adenine nucleotides]. | 1968 | 5727591 | |
| mechanism of overproduction of orotic acid by a mutant of brevibacterium ammoniagenes. | 1969 | 5774759 | |
| the capacity of orotic acid production in pyrimidine-deficient mutants of brevibacterium ammoniagenes. | 1969 | 5786385 | |
| numerical taxonomy of some named coryneform bacteria. | 1969 | 5792675 | |
| the special physiology of hydrocarbon oxidizing bacteria. | 1969 | 5795917 | |
| divergent metabolic pathways for propane and propionate utilization by a soil isolate. | the metabolism of propane and propionate by a soil isolate (brevibacterium sp. strain job5) was investigated. the presence of isocitrate lyase in cells grown on isopropanol, acetate, or propane and the absence of this inducible enzyme in n-propanol- and propionate-grown cells suggested that propane is not metabolized via c-terminal oxidation. methylmalonyl coenzyme a mutase and malate synthase are constitutive in this organism. the incorporation of (14)co(2) into pyruvate accumulated during prop ... | 1969 | 5802607 |
| concerted inhibition and its reversal by end products of aspartate kinase in brevibacterium flavum. | 1969 | 5811784 | |
| pyruvate dependent adenyl cyclase activity of brevibacterium liquefaciens. | 1965 | 5865503 | |
| coryneform bacteria in poultry, eggs and meat. | 1966 | 5949430 | |
| enzymic formation of deoxyadenosine 3',5'-phosphate. | 1966 | 5967096 | |
| influence of food microorganisms on staphylococcal growth and enterotoxin production in meat. | forty-four microorganisms were studied for their influence on staphylococcal growth and enterotoxin production. inhibition was found to be more common than stimulation. two types of inhibition were observed: inhibition of staphylococcal growth, and inhibition of enterotoxin formation with no apparent effect on growth. by use of a plate test, 12 of the 44 food microorganisms were found to inhibit staphylococcal growth at 35 c. of the 12, 3 also inhibited growth at 25 c. no significant differences ... | 1966 | 5970822 |
| glutamate kinase activity in brevibacterium flavum: relationship between l-proline and l-glutamine biosynthesis. | 1967 | 6035481 | |
| formation of adenosine cyclic 3',5'-phosphate by nonproliferating cells and cell-free extract of brevibacterium liquefaciens. | the formation of adenosine cyclic 3',5'-phosphate by brevibacterium liquefaciens atcc 14929 was studied with the use of nonproliferating cells and cell-free extract. with nonproliferating cells provided by deprivation of sulfate, the formation of this nucleotide was accelerated by adding some amino acids and sugars. among amino acids tested, alanine and asparagine were most effective. pentoses were more favorable than hexoses and other sugars. formation of adenosine cyclic 3',5'-phosphate was ob ... | 1967 | 6039354 |
| purification and properties of bacterial phosphopyruvate hydratase. | 1967 | 6048971 | |
| the biosynthesis of 1,6-phenazinediol 5,10-dioxide (iodinin) by brevibacterium iodinum. | 1967 | 6055186 | |
| malate dehydrogenases. i. a survey of molecular size measured by gel filtration. | 1967 | 6069046 | |
| effect of substrate on the fatty acid composition of hydrocabon-utilizing microorganisms. | the fatty acid pattern in three hydrocarbon-utilizing bacteria during growth on various substrates was examined. the predominant fatty acids in acetate-grown cells were c(16), c(16:1), c(18:1), and br-c(19) and the major fatty acids in propane-grown cells were c(15), c(17), c(17:1), c(18:1), and br-c(18). when one organism (mycobacterium sp. strain ofs) was grown on the n-alkanes from c(13) to c(17), the major fatty acid in the cells was of the same chain length as the substrate. studies on the ... | 1967 | 6074400 |
| electrochemical proton gradient of brevibacterium linens and its relationship to phenylalanine transport. | the proton motive force generated by brevibacterium linens was determined by the accumulation of radiolabelled tetraphenylphosphonium for transmembrane potential (delta psi) and by the accumulation of benzoate ions for the h+ chemical gradient (delta ph). in resting cells at ph 8.0, the delta psi was 172 +/- 10 mv while delta ph was 9 mv. the additions of valinomycin to b. linens in the presence of 100 mm exogenous potassium reduced the delta psi and led to a drastic inhibition of phenylalanine ... | 1984 | 6095716 |
| purification and properties of bifunctional 3-deoxy-d-arabino-heptulosonate 7-phosphate synthetase-chorismate mutase component a from brevibacterium flavum. | 1980 | 6104656 | |
| effect of enzyme concentration on regulation of dissociable chorismate mutase in brevibacterium flavum. | 1981 | 6115857 | |
| the metabolic fate of 14c-labeled immunoadjuvant peptidoglycan monomer. ii. in vitro studies. | peptidoglycan monomer (glcnac-murnac-l-ala-d-isoglutamine-meso-diaminopimelic acid-d-ala-d-ala), labeled with 14c both in the disaccharide and pentapeptide portions, was incubated with slices of mouse liver, kidney or spleen as well as with mouse and human blood, blood cells plasma and serum. peptidoglycan monomer was isolated unchanged after incubations with mouse organs and blood cells. however, upon incubation with mouse or human blood, 10-50% of the peptidoglycan monomer underwent hydrolysis ... | 1981 | 6118181 |
| [biosynthesis of glutamic acid by a brevibacterium flavum 585 mutant]. | 1982 | 6128868 | |
| [biosynthesis of glutamic acid by a brevibacterium flavum 258-906 mutant on molasses media]. | 1982 | 6128869 | |
| microbial production of l-[15n]glutamic acid and its gas chromatography-mass spectrometry analysis. | l-[15n]glutamic acid was prepared in high yields via a fermentative process. brevibacterium lactofermentum, growing on a medium containing 97% enriched 15nh4cl as a sole isotopic precursor, excreted mostly l-[15n]glutamic acid. the l-[15n]glutamic acid was purified and identified. gas chromatography-mass spectrometry analysis was performed to demonstrate its usefulness in clinical studies. | 1983 | 6137971 |
| protoplast transformation of glutamate-producing bacteria with plasmid dna. | a method for polyethylene glycol-induced protoplast transformation of glutamate-producing bacteria with plasmid dna was established. protoplasts were prepared from cells grown in the presence of penicillin by treatment with lysozyme in a hypertonic medium. the concentration of penicillin during growth affected the efficiency of formation, regeneration, and polyethylene glycol-induced dna uptake of protoplasts. regeneration of protoplasts was accomplished on a hypertonic agar medium containing so ... | 1984 | 6145700 |
| parameters of unbalanced growth and reversible inhibition of deoxyribnucleic acid synthesis in brevibacterium ammoniagenes atcc 6872 induced by depletion of mn2+. inhibitor studies on the reversibility of deoxyribonucleic acid synthesis. | unbalanced growth induced by depletion of manganese ions was a prerequisite for production of ribonucleotides in a high salt mineral medium with the wildtype strain brevibacterium ammoniagenes atcc 6872. the concentration of manganese strictly controlled the overall deoxyribonucleic acid (dna) synthesis, whereas ribonucleic acid (rna), protein and cell wall synthesis remained essentially unimpaired in the manganese-lacking cells. the reversibility of inhibition of overall dna synthesis was shown ... | 1980 | 6158925 |
| [stimulating effect of complexamine on the growth of the lysine producer brevibacterium sp. 22-l]. | 1981 | 6173733 | |
| nucleic acid hybridization studies on microbacterium, curtobacterium, agromyces and related taxa. | thirty strains of agromyces, arthrobacter, curtobacterium, brevibacterium, corynebacterium and microbacterium, exhibiting the rare peptidoglycan of group b, were subjected to extensive nucleic acid hybridization studies. the dna homology values indicate that corynebacterium insidiosum dsm 20157 is genetically identical with corynebacterium michiganense dsm 20134. corynebacterium sepedonicum ncppb 378 and corynebacterium nebraskense dsm 20400 are closely related to corynebacterium michiganense ds ... | 1982 | 6183393 |
| a phylogenetic analysis of the family dermatophilaceae. | the comparative analysis of the 16s ribosomal ribonucleic acid (rrna) of geodermatophilus obscurus dsm 43060 and dermatophilus congolensis dsm 43037 revealed that these members of the family dermatophilaceae were only remotely related. while g. obscurus represented an individual and separate line of descent within the phylogenetically defined order actinomycetales, d. congolensis was closely related to representatives of arthrobacter, micrococcus, cellulomonas, brevibacterium, promicromonospora ... | 1983 | 6195302 |
| metabolism of pyrimidine bases and nucleosides in the coryneform bacteria brevibacterium ammoniagenes and micrococcus luteus. | the metabolism of exogenous pyrimidine bases and nucleosides was investigated in brevibacterium ammoniagenes and micrococcus luteus with fluorinated analogs and radioactive precursors. salvage of thymine and thymidine was found in m. luteus, but not in b. ammoniagenes. exogenous uracil or uracil nucleosides, but not cytosine or cytosine nucleosides, were nucleic acid precursors for both bacteria. by examining the possible nucleoside-metabolizing enzymes, it can be suggested that the pyrimidine s ... | 1984 | 6202675 |
| skin microbiology: coming of age. | 1984 | 6229637 | |
| role of subunit interactions in the self-assembly of oligomeric proteins. | in oligomeric proteins, the native conformation and its functional properties depend on the interactions which exist between the different chains. the role of these subunit interactions can be studied using either the unfolded state or the native state as a starting point. during the folding process, the properties which appear following a bimolecular reaction are related to the formation of an association area. similarly, the properties which are lost upon partial dissociation of the native sta ... | 1984 | 6242326 |
| mechanism of the adenylate cyclase reaction. stereochemistry of the reaction catalyzed by the enzyme from brevibacterium liquefaciens. | adenylate cyclase from brevibacterium liquefaciens (atcc 14929) catalyzes the formation of the rp-diastereomer of adenosine 3':5'-cyclic monophosphorothioate from the sp-diastereomer of adenosine-5'-(1-thiotriphosphate). the reaction catalyzed by this adenylate cyclase proceeds with inversion of configuration at phosphorus, indicating that the cyclization reaction is direct and does not involve formation of an adenylated enzyme intermediate. | 1980 | 6243273 |
| preparation, characterization, and coenzymic properties of 5-carba-5-deaza and 1-carba-1-deaza analogs of riboflavin, fmn, and fad. | 1980 | 6246390 | |
| [determination of the dinucleosome structure of chromatin by the action of exogenous endonuclease]. | 1981 | 6262034 | |
| [specific effect of endonucleases from brevibacterium ammoniagenes on dna]. | the previously described deoxyribonucleases from brevibacterium ammoniagenes have been characterized. it was shown that they are endonucleases with molecular weights of 60 000 (i), 10 000 (ii) and 20 000 (iii). the rate of endonuclease i effect on native dna exceeded that on the denatured dna 2-fold. the mechanism of its action is of a single hit type. the enzyme hydrolyzes two chains of dna simultaneously in two symmetrical sites and splits the bond 5'-p to form fragments with terminal 5'-oh an ... | 1981 | 6268197 |
| incorporation of deoxyribonucleosides into dna of coryneform bacteria and the relevance of deoxyribonucleoside kinases. | in order to obtain basic knowledge of the salvage pathways for dna synthesis, the ability of brevibacterium ammoniagenes atcc 6872 and micrococcus luteus atcc 15932 for incorporation of nucleobases and nucleosides was investigated. only adenine and uracil are incorporated by b. ammoniagenes, whereas m. luteus additionally can utilize deoxyadenosine and, less efficiently, thymidine. in m. luteus, the demonstration of deoxyadenosine kinase and thymidine kinase explains the incorporation data. upta ... | 1982 | 6277626 |
| a high yield method for the preparative synthesis of coenzyme a by combination of chemical and enzymic reactions. | dried cells of brevibacterium ammoniagenes are a good enzyme source for the preparative synthesis of coa from pantothenic acid, l-cysteine and atp. a problem with this synthesis is that the coa synthesis is repressed by negative feedback inhibition by coa to pantothenate kinase, the first step enzyme for the biosynthesis of coa, which catalyses phosphorylation of pantothenic acid or pantetheine. as the inhibition operates only at this step, a further increased yield is possible if the enzymic ph ... | 1983 | 6299790 |
| brevibacterium fuscum protocatechuate 3,4-dioxygenase. purification, crystallization, and characterization. | a protocatechuate 3,4-dioxygenase with exceptionally sharp spectral features and a new subunit composition has been purified and crystallized from the gram-positive organism brevibacterium fuscum. epr spectra show that the catalytically essential fe3+ resides in a site of almost the maximal rhombicity (e/d = 0.333 +/- 0.003). the spectral line widths (1.4 millitesla at g = 9.67) are the smallest reported for any biological high spin fe3+ complex and suggest that the enzyme is quite homogeneous i ... | 1984 | 6323474 |
| transition state analogs for protocatechuate 3,4-dioxygenase. spectroscopic and kinetic studies of the binding reactions of ketonized substrate analogs. | the binding reactions of two heterocyclic analogs of protocatechuate (pca), 2-hydroxyisonicotinic acid n-oxide and 6-hydroxynicotinic acid n-oxide, to brevibacterium fuscum protocatechuate 3,4-dioxygenase have been characterized. these analogs were synthesized as models for the ketonized tautomer of pca which we have previously proposed as the form which reacts with o2 in the enzyme complex (que, l., jr., lipscomb, j.d., munck, e., and wood, j.m. (1977) biochim. biophys. acta 485, 60-74). both a ... | 1984 | 6323475 |
| 17o-water and cyanide ligation by the active site iron of protocatechuate 3,4-dioxygenase. evidence for displaceable ligands in the native enzyme and in complexes with inhibitors or transition state analogs. | hyperfine broadening is observable in the epr spectrum of brevibacterium fuscum protocatechuate 3,4-dioxygenase after lyophilization and rehydration in 17o-enriched water, demonstrating h2o ligation to the active site iron. lack of detectable broadening in the sharp features of the spectra of three substrate complexes suggests that h2o is displaced by substrate. water is bound in the monodentate complex with the competitive inhibitor 3-hydroxybenzoate which binds directly to the iron showing tha ... | 1984 | 6323476 |
| identification of brevibacterium from clinical sources. | coryneform bacteria of the genus brevibacterium occur on the normal skin surface, but reports of human infection with this genus are lacking. a number of cultures of coryneform bacteria sent to the national collection of type cultures for identification have been identified as brevibacterium spp on the basis of their cell wall composition and ability to produce methane-thiol from l-methionine. we describe a rapid method for the detection of methane-thiol and confirmatory tests which differentiat ... | 1984 | 6392351 |
| purification and properties of a nad-linked 1,2-propanediol dehydrogenase from propane-grown pseudomonas fluorescens nrrl b-1244. | nad-dependent 1,2-propanediol dehydrogenase (ec 1.1.1.4) activity was detected in cell-free crude extracts of various propane-grown bacteria. the enzyme activity was much lower in 1-propanol-grown cells than in propane-grown cells of pseudomonas fluorescens nrrl b-1244, indicating that the enzyme may be inducible by metabolites of propane subterminal oxidation. 1,2-propanediol dehydrogenase was purified from propane-grown ps. fluorescens nrrl b-1244. the purified enzyme fraction shows a single-p ... | 1983 | 6407398 |
| [effect of molasses on an inoculum culture of a lysine producer]. | 1983 | 6408361 | |
| microbial fermentative preparation of l-[15n2]lysine and its tracer: application to serum amino acid kinetic studies. | the microorganism brevibacterium flavum 21129 has been used to produce multigram batches of l-[15n2]lysine of high purity and isotopic enrichment by supplementation of the growth medium with (15nh4)2so4 of 98.0 atom% excess. the doubly 15n-labeled lysine can be detected at dilutions 10 times greater than singly labeled lysine when isotope dilution curves are analyzed by gas chromatography-mass spectrometry. this enhanced sensitivity permits kinetic measurements of plasma free-lysine isotope cont ... | 1983 | 6412590 |
| identification key for coryneform bacteria derived by numerical taxonomic studies. | six main groups were formed from a complete linkage dendrogram on 557 bacteria tested for 53 physiological features. the organisms were obtained from culture collections and included representatives of the following genera: arthrobacter, brevibacterium, caseobacter, cellulomonas, corynebacterium, curtobacterium, micrococcus, microbacterium, mycobacterium, nocardia, oerskovia and rhodococcus. the six groups were individually subjected to a numerical taxonomic analysis based on linkage maps, which ... | 1983 | 6413644 |
| [effect of technical threonine sources on homoserine biosynthesis by mutant brevibacteruim flavum 2t]. | the effect of threonine technical sources on the homoserine biosynthesis by the threonine auxotroph brevibacterium flavum 2t when cultivated on sucrose and acetic acid containing media was investigated. various threonine sources (corn extract and fodder yeast, microbial biomass and soybean meal hydrolyzates) prepared by means of different hydrolyzing agents (acids, enzymes, autolysis) were used. the most effective substrate was protein--vitamin concentrate hydrolyzate, particularly combined with ... | 1983 | 6413968 |
| [cyanide-resistant oxygen consumption of the lysine-synthesizing bacteria brevibacterium flavum 22 ld]. | the growth of the culture and biosynthesis of lysin were studied in brevibacterium flavum 22 ld cultivated in a chemostat. during cultivation the flow rate of the medium and the partial pressure of oxygen dissolved in the medium were varied. the maximum yield of lysine, calculated in respect to the sucrose consumed, (yp = g lysine . hcl/g sucrose) was registered when cyanide-resistant oxygen consumption was the least. a change of the cultivation conditions provoked a decrease of yp value and a s ... | 1984 | 6422458 |
| [taxonomic position of the lysine producer brevibacterium flavum]. | brevibacterium flavum 22 and 22l producing lysine and glutamic acid should be reclassified as corynebacterium glutamicum on the basis of their chemotaxonomic characteristics: the iv type of the cell wall, corynomycolic acids c32--c34, 57.8% of gc in dna. | 1984 | 6423939 |
| [free mycolic acids of the cells of coryneform and nocardia-like bacteria]. | the composition of free mycolic acids was studied in the cells of brevibacterium ammoniagenes atcc 6871, b. flavum 22, b. stationis atcc 14403, corynebacterium divaricatum atcc 14020 and rhodococcus maris imv 195. the acids are a mixture of saturated and unsaturated compounds with the total number of carbon atoms from 32 to 36 and the number of c atoms in the alpha-chain from 10 to 15. | 1984 | 6431238 |
| role of biotin in the production of lysine by brevibacterium lactofermentum. | to investigate the role of biotin in lysine production, brevibacterium lactofermentum atcc 21086 was grown in an acid-hydrolysed whey permeate medium with and without added biotin. added biotin stimulated lysine production and growth of b. lactofermentum. five micrograms of biotin/100 ml was the optimum level of addition. biotin increased the uptake of 14c-glucose and affected fatty acid composition of cell wall lipids. cell walls of test organisms contained less 16:0 and more 18:2 fatty acids t ... | 1984 | 6434904 |
| bacterial growth in artificial capillary spaces. | in experiments on the influence of microscopic capillaries on the growth of escherichia coli, brevibacterium linens, micrococcus flavus and bacillus cereus, a distinct delay in growth was observed. the difference in counts was greatest after 8 h. later a slight equilibration of counts was noted. with b. cereus, only slight or no spore formation was observed under microcapillary conditions. | 1984 | 6437860 |
| [selection of bacterial strains for the production of threonine]. | the production of l-threonine in submerged culture was studied in the following bacterial strains; brevibacterium flavum atcc 21269, corynebacterium acetoacidophilum atcc 21270, escherichia coli atcc 21149, e. coli nrrl 12098, e. coli nrrl 12099 and e. coli nrrl 12100. erlenmeyer flasks with different volumetric relations liquid/recipient, were used to study the influence of the volumetric oxygen transfer rate. b. flavum reached levels of threonine of 0.72 g/l at 96 hours of culture with a volum ... | 1984 | 6443832 |
| [nicotinamide-adenine dinucleotide synthesis by microorganisms]. | the ability of five bacterial strains, i.e., brevibacterium ammoniagenes atcc 6872, brevibacterium flavum atcc 14067, brevibacterium 22, corynebacterium atcc 21084, micrococcus glutamicus atcc 13032, to utilize exogenous precursors (nicotinamide and adenine or atp) was investigated during nad synthesis under fermentation conditions and during incubation of acetone-dried cells. it was found that dry cells of brevibacterium three strains were most active. however, under fermentation conditions br. ... | 1981 | 6459575 |
| [effect of the surface-active substance n-cetylpyridinium chloride on the biosynthetic capacity of brevibacterium ammoniagenes atcc 6872, a producer of nad]. | the effect of cetylpyridinium chloride (cpc), a cationic detergent, on the biosynthetic ability of the well known synthesizor of nucleotides brevibacterium ammoniagenes atcc 6872 was studied. it was found that cpc increasing the cell permeability affects also the cell carbohydrate metabolism. at the definite ratio of cpc and biomass which depends on the composition of the incubating medium, a maximum formation of pentoses occurs that is of great importance for obtaining nad from the exogeneous p ... | 1984 | 6463036 |
| peptidoglycans as promoters of slow-wave sleep. ii. somnogenic and pyrogenic activities of some naturally occurring muramyl peptides; correlations with mass spectrometric structure determination. | the structures of components of the sleep-promoting material purified from human urine were established by fast atom bombardment-mass spectrometry, as reported in the accompanying paper (martin, s. a., karnovsky, m. l., krueger, j. m., pappenheimer, j. r., and biemann, k. (1984) j. biol. chem. 259, 12652-12658). we report here that two substances isolated from that preparation, viz. n-acetylglucosaminyl-1,6 -anhydro-n-acetylmuramyl-ala-gamma-glu-diaminopimelyl-ala) and that compound lacking the ... | 1984 | 6490637 |
| immunotherapy of lewis lung carcinoma with hydrosoluble peptidoglycan monomer (pgm). | the water-soluble peptidoglycan monomer (pgm) isolated from the culture fluid of brevibacterium divaricatum, which has immunostimulating activity, has been examined for its antitumor effects in c57bl mice bearing lewis lung carcinoma. the formation of spontaneous lung metastases from sc tumor implants is significantly inhibited. the growth of sc primary tumors, including advanced ones, is also significantly inhibited, though to a less pronounced extent than the growth of metastases. the effects ... | 1983 | 6553516 |
| in vivo 15n nmr studies of regulation of nitrogen assimilation and amino acid production by brevibacterium lactofermentum. | glutamic acid producer brevibacterium lactofermentum intact cells were used to demonstrate the feasibility of in vivo 15n nmr to follow nitrogen assimilation and amino acid production throughout the growth cycle. the induction of glutamic acid production by different growth conditions was studied. intracellular and extracellular levels of free metabolites were estimated as function of oxygen supply and biotin concentration. 15n nmr enabled us to distinguish two phases during the fermentation. at ... | 1983 | 6630214 |
| [effect of antibiotics on 5'-inosinic acid biosynthesis by a brevibacterium ammoniagenes mutant]. | the effect of streptomycin, erythromycin, kanamycin and penicillin on the biosynthesis of 5'-inosinic acid (imp) by the mutant strain brevibacterium ammoniagenes was studied. it has been found that the efficiency of antibiotic action depends not only in its concentration but on the age of the culture. when the antibiotics were introduced into the culture broth at the beginning of fermentation, they inhibited the culture growth and accumulation of imp in the cultural medium. only after 36-72 hour ... | 1983 | 6647419 |
| [coordinated regulation of the enzyme activity of glucose transport and metabolism in brevibacterium flavum]. | a coordination was established between the rate of glucose transport and the activity of enzymes involved in glycolysis and the citric acid cycle (cac) in brevibacterium flavum cells producing lysine: the rate of glucose transport and the activity of enzymes catalysing glycolysis increased when the activity of cac enzymes decreased and vice versa. the activity of glycolytic enzymes and the rate of glucose transport in the cells increased also when electron transport through the respiration chain ... | 1983 | 6664311 |
| immunotherapy of b-16 melanoma with peptidoglycan monomer. | b-16 melanoma-bearing mice received intravenously or intratumorally one or multiple injections of peptidoglycan monomer (pgm) derived from brevibacterium divaricatum cell wall. multiple injections of this non-toxic, water-soluble, low-molecular-weight peptidoglycan reduced the growth rate of tumor nodule on the leg, but did not significantly prolong the survival of tumor-bearing mice. one milligram of pgm administered 3 or 7 days after tumor inoculation inhibited formation of pulmonary metastase ... | 1983 | 6683639 |
| [transport of phenylalanine and tyrosine in brevibacterium linens: specificity and incorporation into proteins]. | the specificity of phenylalanine and tyrosine carriers was investigated using actively metabolizing cells of brevibacterium linens. the cellular protein synthesis of resting cells was very weakly inhibited, even with high concentrations of chloramphenicol or tetracycline. the nonaromatic amino acids were weak inhibitors for these carriers, while fluorinate analogues of phenylalanine and tyrosine were very potent competitive inhibitors. in practice these analogues cannot be used to replace amino ... | 1984 | 6744119 |
| [production of genetic recombinants in brevibacterium ammoniagenes and brevibacterium divaricatum by protoplast fusion]. | 1982 | 6757055 | |
| differential hydrogen exchange during the fatty acid synthetase reaction: deuterium distribution of fatty acids synthesized from [2-2h2]malonyl-coa. | 1982 | 6758778 | |
| comparative studies on the regulation of tryptophan synthesis. | in vitro dna recombination techniques have revolutionized the study of genetic control of biosynthetic pathways. using examples drawn from the pathway of tryptophan synthesis, approaches to the deciphering of regulatory signals and response mechanisms through transposition of dna segments and dna sequence analysis will be presented. after reviewing the known chromosomal arrangements and regulatory patterns of trp genes in the bacterial groups studied so far, and describing the results of transfe ... | 1980 | 6772375 |
| [carbohydrate metabolic characteristics of coryneform bacteria]. | the peculiarities of glucose metabolism were studied in typical representatives of coryneform bacteria, and its relation to inorganic polyphosphate metabolism was shown. the activity of the first two enzymes in the pentose pathway was found to be low. the key enzymes of glycolysis were detected, viz. fructose-1,6-diphosphate aldolase and 3-phosphoglyceraldehyde dehydrogenase. the second enzyme was more active in nocardia sp. b-293 similar in its properties to nocardia erythropolis as compared to ... | 1980 | 6774212 |
| fatty acid, isoprenoid quinone and polar lipid composition in the classification of curtobacterium and related taxa. | strains representing the taxa curtobacterium, brevibacterium saperdae, b. testaceum, corynebacterium betae, cor. nebraskense and cor. oortii were degraded by acid methanolysis and the non-hydroxylated fatty acid esters released were examined by thin-layer and gas-liquid chromatography. the major fatty acids in all strains were 12-methyltetradecanoic (anteiso c15) and 14-methylhexadecanoic (anteiso c17) acids which occurred together with other anteiso, iso and straight-chain acids. polar lipids o ... | 1980 | 6775048 |
| [ratio of coryneform bacteria to the organic substance concentration]. | the effect of various organic substances and their concentrations on growth was studied with 10 strains of coryneform bacteria belonging to different taxonomic groups. the dynamics of the cultural growth depended on the nature of a substrate, glucose or acetate. arthrobacter globiformis 281, a. variabilis 289, nocardia erythropolis 236, n. globerula 502, n. minima 311 and n. rhodochrous 435 had a higher growth rate on acetate than on glucose. a. pascens 284, brevibacterium ammoniagenes 334, cory ... | 1980 | 6782432 |
| [development cycles of coryneform and nocardia-like bacteria]. | the growth cycles and the types of cell separation were studied in a microchamber with the collection strains of brevibacterium ammoniagenes attc 6871, b. helvolum atcc 19239, b. linens ccm 47 and atcc 9174, b. maris vkm b-464 and b. stationis atcc 14403, as well as with the strains of the genus rhodococcus isolated from soils, viz. r. maris sp. nov. imb 283 and r. luteus sp. nov. imb 385. according to the increasing complexity of cellular morphological transformation in the life cycle, the orga ... | 1980 | 6782438 |
| cell wall composition in the classification and identification of coryneform bacteria. | 1980 | 6792712 | |
| [differentiation of the genera of coryneform bacteria synthesizing amino acids and nucleotides]. | the morphological, cultural, physio-biochemical and chemotaxonomic properties as well as the content of gc in dna were studied in coryneform bacterial strains producing amino acids and nucleotides. it has been shown that brevibacterium ammoniagenes vkm 672, b. flavum 317a, b. stationis ccm 317 and corynebacterium vsti 301 should be assigned to the genus corynebacterium. the taxonomic significance of chemotaxonomic and physio-biochemical properties is discussed on the basis of the results obtaine ... | 1982 | 6803106 |
| [functional characteristics of phosphoenolpyruvate carboxylase in bacteria producing lysine]. | phosphoenol pyruvate carboxylase, or pep-c (ec 4.1.1.31), was shown to be the only enzyme catalyzing anaplerotic synthesis of oxalacetic acid in brevibacterium flavum synthesizing lysine. acetyl-coa is required for the operation of pep-c in the strains. changes in the activity of pep-c did not entirely correlate with those of the citric acid cycle enzymes. hence, pep-c is involved not only in the citric acid cycle, but also in other functions of the cell. a correlation has been found between cha ... | 1982 | 6803109 |
| [carbon isotope fractionation by aerobic heterotrophic microorganisms]. | the isotope effects of carbon were studied in auxotrophic mutants of the following aerobic microorganisms: escherichia coli, corynebacterium, bacillus subtilis, brevibacterium and micrococcus glutamicus. intramolecular isotope heterogeneity was found in position of the total carbon differed from that of the carbon of the carboxyl in the amino acid. in the lysine released by corynebacterium grown on acetate, the carboxyl carbon is enriched with 13c by 8%o comparing with the total carbon of the am ... | 1982 | 6806574 |
| [effect of the carbon source and aeration conditions on homoserine lysine biosynthesis in the threonine-dependent mutant brevibacterium flavum 2t]. | the effect of two carbon sources (sucrose and acetate), aeration conditions and threonine concentration on the homoserine and lysine biosynthesis by the threonine-dependent mutant brevibacterium flavum 2t was examined. it was demonstrated that acetate provided the predominant synthesis of homoserine to a far greater extent than sucrose (with the weight/weight ratio of homoserine : lysine being 2.5-5.0 and 0.8-1,2, respectively). the maximal level of homoserine and lysine was 18-21 and 3-7 g/l on ... | 1982 | 6808495 |
| patterns of nucleotide utilisation in bacterial succinate thiokinases. | 1982 | 6811321 | |
| numerical classification of some rhodococci, corynebacteria and related organisms. | nineteen strains of corynebacterium sensu stricto, 23 received as corynebacterium equi or rhodococcus equi, marker cultures of arthrobacter, brevibacterium, bacterionema matruchotii, cellulomonas flavigena, kurthia zopfii, listeria denitrificans, microbacterium lacticum, rhodococcus rubropertinctus and 88 representatives of mycobacterium, nocardia, rhodococcus and the 'aurantiaca' taxon were the subject of numerical phenetic analyses using 92 characters. the data were examined using the simple m ... | 1982 | 6811696 |
| product biosynthesis in continuous fermentation. | 1982 | 6815034 | |
| transport of aromatic amino acids by brevibacterium linens. | whole metabolizing brevibacterium linens cells were used to study the transport of aromatic amino acids. kinetic results followed the michaelis-menten equation with apparent km values for phenylalanine, tyrosine, and tryptophan of 24, 3.5, and 1.8 microm. transport of these amino acids was optimum at ph 7.5 and 25 degrees c for phenylalanine and ph 8.0 and 35 degrees c for tyrosine and tryptophan. crossed inhibitions were all noncompetitive. the only marked stereospecificity was for the l form o ... | 1983 | 6885717 |
| [incorporation of adenine derivatives into the cells of brevibacterium ammoniagenes gana mutants producers of inosinic acid]. | the gana mutants (growth ability with nucleotides), overproducers of inosinic acid, were isolated with the aid of specially developed techniques aimed at obtaining mutants with the increased ability of assimilating adenine derivatives. the selection techniques used, as well as certain properties of gana mutants distinguishing them from their parent strains auxotropic for adenine, led to suggesting that gana mutation affects cell permeability for exogenous adenine derivatives. the present paper r ... | 1982 | 6890490 |
| arogenate (pretyrosine) is an obligatory intermediate of l-tyrosine biosynthesis: confirmation in a microbial mutant. | wild-type brevibacterium flavum has been shown to possess arogenate dehydrogenase activity and to lack prephenate dehydrogenase, thereby providing presumptive evidence that arogenate (previously named "pretyrosine") is an obligatory intermediate of l-tyrosine biosynthesis. a similar enzymological pattern has been discerned in extracts made from wild-type cultures of various species of cyanobacteria. application of rigorous molecular genetic criteria in confirmation of the exclusive role of aroge ... | 1980 | 6929482 |
| more on substrate specificity of cholesterol oxidase. | 1980 | 6934039 | |
| transmembrane movement of cholesterol in small unilamellar vesicles detected by cholesterol oxidase. | greater than 90% of the cholesterol in small unilamellar vesicles composed of egg lecithin and cholesterol (molar ratio 1:0.7) was oxidized by a cholesterol oxidase from brevibacterium sp., with a single time constant and a half-time of 1 min at 37 degrees c. the enzyme preparation used was at least 95% pure and possessed no detectable phospholipase c activity. since cholesterol is present in both halves of the bilayer, it was concluded that transmembrane movement of cholesterol in these vesicle ... | 1981 | 6935193 |
| cerulenin. | 1981 | 7031426 | |
| a new manganese-activated ribonucleotide reductase found in gram-positive bacteria. | 1981 | 7032519 | |
| steric course of deuterium incorporation from [2-2h2]malonyl-coa into fatty acids by fatty acid synthetases. | the steric course of the enoyl reduction catalyzed by fatty acid synthetase was investigated with the enzymes from bakers' yeast, rat liver and brevibacterium ammoniagenes. the non-enzymic hydrogen-deuterium exchange of the methylene group of malonyl-coa was studied by nmr spectroscopy. the half-life period of the methylene protons was 4.8 min at 37 degrees c and 12.2 min at 23 degrees c at p2h 7.5. deuterium-labeled fatty acids were synthesized by incubating the synthetases with [2-2h2]malonyl- ... | 1981 | 7037760 |
| propionyl-coa induced synthesis of even-chain-length fatty acids by fatty acid synthetase from brevibacterium ammoniagenes. | the product distribution of brevibacterium ammoniagenes fatty acid synthetase has been investigated using propionyl-coa instead of acetyl-coa as the primer. the synthetase produces not only an odd-numbered fatty acid (heptadecanoic acid) but also even-numbered fatty acids (stearic and oleic acids) in the presence of propionyl-coa. the amounts of heptadecanoic, stearic and oleic acids increased with increasing concentration of propionyl-coa. however, the formation of heptadecenoic acid (c17:1) wa ... | 1982 | 7068555 |
| [study of nucleotide biosynthesis by cultured brevibacterium ammoniagenes using p31 nuclear magnetic resonance spectroscopy]. | using p31 nuclear magnetic resonance, transformations of phosphorous compounds by the culture brevibacterium ammoniagenes atcc 6872 in the course of fermentation aimed at the synthesis of nicotinamide adenine dinucleotide were investigated. it was found that the nad synthesis by the cells treated with surface active substances was markedly influenced by the presence of a small (initial) atp quantity and was unaffected by an addition of the synthetic polyphosphate (n-40). it was demonstrated that ... | 1982 | 7079251 |
| a survey of the structures of mycolic acids in corynebacterium and related taxa. | acid methanolysates of 104 strains of coryneform and related bacteria were examined for mycolic acids by thin-layer chromatography and mass spectrometry. the mycolic acids of corynebacterium sensu stricto strains were not uniform in size and structure, and considerable variation existed within the approximate limiting range of c22 to c36. two species, corynebacterium bovis and 'corynebacterium mycetoides', were particularly distinctive; the former had exceptionally low molecular weight mycolic a ... | 1982 | 7086391 |
| a preferential isolation procedure for asporogenous gram-positive bacteria. | a preferential isolation procedure was devised for asporogenous (asp), gram-positive (gp), aerobic or facultative anaerobic bacteria which included the genera arthrobacter, corynebacterium, brevibacterium, microbacterium, mycobacterium, and micrococcus (asp-gp bacteria). an antibiotics-mixture agar which contained 5 to 10 micrograms per ml of colistin, 10 to 20 micrograms per ml of nalidixic acid and 30 micrograms per ml of cycloheximide was used in the isolation. using this technique 47 asp-gp ... | 1982 | 7096199 |