Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| comparison of cyanide-degrading nitrilases. | recombinant forms of three cyanide-degrading nitrilases, cynd from bacillus pumilus c1, cynd from pseudomonas stutzeri, and cht from gloeocercospora sorghi, were prepared after their genes were cloned with c-terminal hexahistidine purification tags and expressed in escherichia coli, and the enzymes purified using nickel-chelate affinity chromatography. the enzymes were compared with respect to their ph stability, thermostability, metal tolerance, and kinetic constants. the two bacterial genes, b ... | 2005 | 15703908 |
| development of a versatile cassette for directional genome walking using cassette ligation-mediated pcr and its application in the cloning of complete lipolytic genes from bacillus species. | since the invention of the pcr technology, adaptation techniques to clone dna fragments flanking the known sequence continue to be developed. we describe a perfectly annealed cassette available in almost unlimited quantities with variable sticky-and blunt-end restriction enzyme recognition sites for efficient restriction and ligation with the restricted target genomic dna. the cassette provides a 200-bp sequence, which is used to design a variety of cassette-specific primers. the dephosphorylati ... | 2004 | 15722149 |
| heterologous expression, purification, crystallization, x-ray analysis and phasing of the acetyl xylan esterase from bacillus pumilus. | bacillus pumilus ps213 acetyl xylan esterase (axe) acts as an accessory enzyme in the plant cell wall hemicellulose biodegradation pathway. it belongs to the carbohydrate esterase family 7 and hydrolyses the ester linkages of the acetyl groups in position 2 and/or 3 of the xylose moieties of the acetylated xylan fragments from hardwood. the enzyme displays activity towards a broad range of acetylated compounds including the antibiotic cephalosporin-c. in this study we report the heterologous exp ... | 2005 | 15769599 |
| evaluating the formulae for integrated lethality in ethylene oxide sterilization using six different endospore forming strains of bacteria, and comparisons of integrated lethality for ethylene oxide and steam systems. | bacterial endospores from six different species of bacteria were exposed to a spectrum of ethylene oxide (eto) sterilizing conditions. temperature was varied from 40 to 60 degrees c and the ethylene oxide concentration was varied from 300 to 750 mg/l. relative humidity was maintained at 60+/-10% rh. the fraction negative procedure was used to determine the d value for each of the test conditions. bacterial species tested included bacillus atrophaeus atcc # 9372, bacillus smithii atcc # 51232, ba ... | 2005 | 15796136 |
| sugarcane bagasse pulps: biobleaching with commercial cartazyme hs and with bacillus pumilus xylanase. | organosolv (ethanol/water and acetosolv) pulps were treated with bacillus pumilus xylanase for 4, 8, and 12 h and compared with commercial cartazyme hs xylanase-treated pulps. treatment of ethanol/water pulps with b. pumilus xylanase increased viscosity by 40% in 8 h of treatment compared with pulps treated without enzyme. however, acetosolv pulps treated with b. pumilus xylanase lost viscosity. ethanol/water pulps treated with cartazyme had a viscosity of 18.5 cp in 4 h of treatment. in the ace ... | 2005 | 15917597 |
| a marked enhancement in the production of a highly alkaline and thermostable pectinase by bacillus pumilus dcsr1 in submerged fermentation by using statistical methods. | the production of a highly alkaline and thermostable pectinase of bacillus pumilus was optimized in submerged fermentation using plackett-burman design and response surface methodology. three fermentation variables (c:n ratio, k(2)hpo(4), and ph), which were identified to significantly affect pectinase production by plackett-burman design were further optimized using response surface methodology of central composite design (ccd). an over all 34- and 41-fold increase in enzyme production was achi ... | 2006 | 15936940 |
| recurrent isolation of hydrogen peroxide-resistant spores of bacillus pumilus from a spacecraft assembly facility. | while the microbial diversity of a spacecraft assembly facility at the jet propulsion laboratory (pasadena, ca) was being monitored, h2o2-resistant bacterial strains were repeatedly isolated from various surface locations. h2o2 is a possible sterilant for spacecraft hardware because it is a low-temperature process and compatible with various modern-day spacecraft materials, electronics, and components. both conventional biochemical testing and molecular analyses identified these strains as bacil ... | 2005 | 15941382 |
| [secreted expression of bacillus pumilus xylanase gene in pichia pastoris and study on enzymatic properties]. | the endo-1,4-xylanase gene from bacillus pumilus hb030 was cloned into the pichia pastoris expression vector, ppic9k, the recombinant plasmid was named phbm220. the digested recombinant plasmid phbm220 was transformed into pichia pastoris km71, gs115, smd1168, respectively. the recombinant pichia pastoris km71 (phbm220), gs115 (phbm220), smd1168 (phbm220) secreted functional endo-1,4-xylanase, and the enzymatic activities reached 10.80iu/ml, 11.63iu/ml, 9.68iu/ml, respectively. the temperature a ... | 2003 | 15969036 |
| a case of persistent bacillus pumilis bacteremia associated with cholangitis. | 2006 | 15992931 | |
| effect of boiling and roasting on the fermentation of soybeans into dawadawa (soy-dawadawa). | soybeans which had initially been dehulled by either boiling (boiled/dehulled) or roasting (roasted/dehulled) before peeling, were cooked and fermented into dawadawa, a traditional food condiment. the micropopulation, enzymatic activities, proximate composition, amino acid, and aroma profiles of the two types of soybean dawadawa were evaluated during fermentation. only minor differences were found in the microbial profiles of the two types of soy-dawadawa. although boiled/dehulled soy-dawadawa i ... | 2005 | 16002169 |
| molecular cloning of enantioselective ester hydrolase from bacillus pumilus dbrl-191. | a gene from bacillus pumilus expressed under its native promoter was cloned in escherichia coli. recombinant b. pumilus esterase (bpe) affects the kinetic resolution of racemic mixtures such as unsubstituted and substituted 1-(phenyl)ethanols (e approximately 33-103), ethyl 3-hydroxy-3-phenylpropanoate (e approximately 45-71), trans-4-fluorophenyl-3-hydroxymethyl-n-methylpiperidine (e approximately 10-13) and ethyl 2-hydroxy-4-phenylbutyrate (e approximately 7). the enzyme is composed of a 34-am ... | 2005 | 16006072 |
| [identification and mode of action of a xylanase a purified from the culture filtrate of bacillus pumilus wl-11]. | microbial xylanases have received a great deal of attention in the last two decades for their potential applications in food, paper making and animal feed industries. bacillus pumilus wl-11 was identified as a producer of alkane xylanase free of cellulase after screening soil samples of paper-making factories. the xylanase a (xyla) was purified to homogeneity from the culture filtrate of bacillus pumilus wl-11 by (nh4) 2so4 precipitation, cm-sephadex and sephadex g-75 chromatographies. the molec ... | 2005 | 16108365 |
| purification and characterization of a new bioscouring pectate lyase from bacillus pumilus bk2. | an alkalophilic bacterium was isolated based on the potential of extra-cellular enzymes for bioscouring. the bacterium was identified as a new strain of bacillus pumilus bk2 producing an extra-cellular endo-pectate lyase pl (ec 4.2.2.2). pl was purified to homogeneity in three steps and has a molecular mass of 37.3+/-4.8 kda as determined by sds-page and an isoelectric point of ph 8.5. peptide mass mapping by nano-lc-ms of pl revealed 15% homology with a pectate lyase from bacillus sp. the pecta ... | 2006 | 16168510 |
| characterization of microorganisms in argentinean honeys from different sources. | seventy polyfloral honeys including commercial samples obtained from supermarkets, harvested from apiaries and purchased in bulk were initially examined for total antibacterial activity. from each sample, numbers of aerobic mesophilic bacteria, total coliforms, moulds and yeasts were determined and the presence of salmonella spp., shigella spp., clostridium sulfite-reducers, paenibacillus larvae and bacillus spp. was investigated. moisture content, ph and total acidity were also determined for a ... | 2005 | 16169624 |
| antibacterial activity of ocimum sanctum l. fixed oil. | ocimum sanctum fixed oil showed good antibacterial activity against staphylococcus aureus, bacillus pumilus and pseudomonas aeruginosa, where s. aureus was the most sensitive organism. sesame and soyabean oils also showed moderate activity against s. aureus. higher content of linolenic acid in o. sanctum fixed oil could contribute towards its antibacterial activity. the antibacterial activity combined with anti-inflammatory and analgesic activities of the oil, could make it useful in inflammator ... | 2005 | 16187537 |
| isolation and characterization of poly(butylene succinate-co-butylene adipate)-degrading microorganism. | poly(butylene succinate-co-butylene adipate) (pbsa)-degrading bacterium, strain 1-a, was isolated from soil. strain 1-a was identified as bacillus pumilus on the basis of its physiological properties and partial 16s rrna gene sequence. strain 1-a also degraded poly(butylene succinate) (pbs) and poly(epsilon-caprolactone). on the other hand, poly(butylene adipate terephthalate) and poly(lactic acid) were minimally degraded by strain 1-a. the nmr spectra of degradation products from pbsa indicated ... | 2004 | 16233604 |
| inhibition of aflatoxin production of aspergillus parasiticus nrrl 2999 by bacillus pumilus. | six isolates of bacillus pumilus were tested for their ability to inhibit aflatoxin production of aspergillus parasiticus nrrl 2999 in yeast extract sucrose (yes) broth. aflatoxin production was inhibited in both simultaneous and deferred antagonism assays, suggesting that the inhibitory activity was due to extracellular metabolite(s) produced in cell-free supernatant fluids of cultured broth. the inhibition was not due to organic acids or hydrogen peroxide produced by b. pumilus since the inhib ... | 1997 | 16284816 |
| volatile compounds of soumbala, a fermented african locust bean (parkia biglobosa) food condiment. | to determine the profile of volatile compounds responsible for the aroma of soumbala produced spontaneously and with pure and mixed cultures of bacillus subtilis and bacillus pumilus. | 2005 | 16313414 |
| survival of spacecraft-associated microorganisms under simulated martian uv irradiation. | spore-forming microbes recovered from spacecraft surfaces and assembly facilities were exposed to simulated martian uv irradiation. the effects of uva (315 to 400 nm), uva+b (280 to 400 nm), and the full uv spectrum (200 to 400 nm) on the survival of microorganisms were studied at uv intensities expected to strike the surfaces of mars. microbial species isolated from the surfaces of several spacecraft, including mars odyssey, x-2000 (avionics), and the international space station, and their asse ... | 2005 | 16332797 |
| stability of bacillus pumilus spore strips used for monitoring radiation sterilization. | [this corrects the article on p. 999 in vol. 31.]. | 1977 | 16345195 |
| identification and characterization of rhizosphere-competent bacteria of wheat. | to obtain rhizosphere-competent bacteria which could subsequently be modified for the development of biological control agents, bacteria were isolated from the rhizosphere and rhizoplane of wheat and barley plants by standard techniques. of these isolates, 60 were selected for field testing as spring wheat seed inoculants in 1985. isolates were marked genetically for resistance to antibiotics via selection of spontaneous mutants to detect and monitor isolates in the field. forty-three days after ... | 1987 | 16347496 |
| one-step purification of a lipolytic enzyme from bacillus pumilus by mild hydrophobic interaction chromatography on polyoxyalkylene glycol-bound sepharose 6b. | an esterase from bacillus pumilus was obtained in a highly purified form, starting from a crude extract, by a new chromatographic technique requiring no detergent during the elution step. the stationary phase involved was mildly hydrophobic and was prepared by covalent immobilization of a polyoxyalkylene glycol onto sepharose 6b. | 1988 | 16347641 |
| mannanase components from bacillus pumilus. | beta-d-mannanase from bacillus pumilus was purified into two components, a and b, which exhibited homogeneity on polyacrylamide gel electrophoresis. these components had molecular weights of 55,000 (a) and 37,000 (b); carbohydrate contents of 15.3% (a) and 7.2% (b); specific activities of 78 (a) and 1,616 (b) u/mg; ph optima of 5.5 to 6.9 (a) and 6.0 (b); and half-lives of 60 (a) and 21 (b) min at 70 degrees c. | 1990 | 16348230 |
| use of a plasmid dna probe to monitor populations of bacillus pumilus inoculant strains in hay. | we are evaluating naturally occurring isolates of bacillus pumilus for use as microbial hay preservatives. seven isolates of b. pumilus from hay contained a 42-kb cryptic plasmid (pmgd296). we wished to determine whether pmgd296 could be used as a molecular marker to follow populations of these isolates in hay over time. southern blots and colony blots of 69 isolates of b. pumilus and other bacillus spp. were probed with p-labeled pmgd296. twenty-nine probe-positive isolates were identified; of ... | 1991 | 16348435 |
| investigation of antimicrobial activity of pelargonium radula (cav.) l'hérit. | antimicrobial activities of two ethanolic extracts, made from fresh and dried leaves of pelargonium radula (cav.) l'hérit, were tested against fourteen species of bacteria and fifteen species of fungi. the well-diffusion method indicated the strongest activity against pseudomonas aeruginosa. the broth dilution method revealed that the most sensitive microorganisms were bacillus pumilus, bacillus subtilis, escherichia coli and serratia marcescens. extract prepared from fresh leaves showed signifi ... | 2005 | 16375830 |
| oligomeric structure of nitrilases: effect of mutating interfacial residues on activity. | nitrilases are important industrial enzymes that convert nitriles into their corresponding acids or, occasionally, amides. atomic resolution structures of four members of the nitrilase superfamily have been determined, but these differ from microbial nitrilases in that they do not form typical large homo-oligomeric complexes. at least two nitrilases, the cyanide dihydratases from pseudomonas stutzeri ak61 and bacillus pumilus c1, form unusual spiral structures of 14 and 18 subunits, respectively ... | 2005 | 16387684 |
| gibberellins-producing rhizobacteria increase endogenous gibberellins content and promote growth of red peppers. | the growth of red pepper plants was enhanced by treatment with the rhizobacterium, bacillus cereus mj-1. red pepper shoots showed a 1.38-fold increase in fresh weight (fw) and roots showed a 1.28-fold fw gain. this plant growth-promoting rhizobacterium (pgpr) has been reported to produce gibberellins (gas). other gas-producing rhizobacteria, bacillus macroides cj-29 and bacillus pumilus cj-69, also enhanced the fw of the plants. they were less effective than b. cereus mj-1, though. the endogenou ... | 2005 | 16410767 |
| antibacterial screening of citrullus colocynthis. | crude ethanolic extracts of fruits, leaves, stems and roots of citrullus colocynthis schrad were examined for their antibacterial potentialities against gram positive and gram negative bacilli. ethanolic extracts of fruits, leaves, stems and roots were found to be active against gram positive bacilli, viz., bacillus pumilus and staphylococcus aureus, while fruit and root extracts in double strength gave positive results against gram positive bacillus (bacillus subtilis). the gram negative bacill ... | 2003 | 16414561 |
| phytochemical and antimicrobial studies of salvia splendens sello. | the phytochemical investigation of antimicrobial agents from locally occurring salvia splendens sello, was carried out. three common solvents i.e. petroleum ether (40-60%), chloroform and methanol were successively used for the extraction of antimicrobial principles from its various parts. three major compounds (namely c1, c2, and c3) were isolated and purified from the active methanol extract of roots by column and thin layer chromatography. gram-positive bacteria (bacillus pumilus, b. subtilis ... | 1998 | 16414814 |
| characterization of bacterial growth and the influence of antibiotics by means of uv resonance raman spectroscopy. | in this work we monitor the bacterial growth of a bacillus pumilus batch culture by means of uv resonance raman spectroscopy. excitation with a wavelength of 244 nm especially enhances the raman scattering of the aromatic amino acids and the nucleic acid bases and therefore is a good method to track the metabolic changes that occur during bacterial growth. furthermore, a drug from the fluoroquinolone group is added to the bacterial suspension at the beginning of the exponential growth phase. wit ... | 2006 | 16421857 |
| phenotypic characterization of food waste degrading bacillus strains isolated from aerobic bioreactors. | a phenotypic characterization of seventeen bacillus strains isolated from aerobic thermophilic bioreactors of a food waste processing company was carried out, using fatty acid and enzymatic activity profiles. it was observed that each species possessed a typical fatty acid and enzymatic production profile. bacillus licheniformis strains exhibited the most significant enzyme production. numerical analyses (principal component and hierarchical cluster analyses) revealed that bacillus licheniformis ... | 2006 | 16463317 |
| identification of nif genes in n2-fixing bacterial strains isolated from rice fields along the yangtze river plain. | the aim of this research was to identify nifh and nifhdkye ' genes in twenty strains of n2-fixing heterotrophic bacteria isolated from rice fields in the yangtze river plain. southern hybridization of the total dna from each strain was performed with the klebsiella pneumoniae nifhdkye ' gene probe (6.2 kb eco ri fragment from psa30) and the azospirillum brasilense nifh gene probe (0.6 kb eco ri-hin diii fragment from phu8). we found that eco ri fragments of total dna from aeromonas hydrophila hy ... | 2006 | 16463319 |
| purification and molecular characterization of subtilisin-like alkaline protease bpp-a from bacillus pumilus strain ms-1. | the present study was conducted by screening zein-degrading bacteria in an attempt to obtain zein-degrading protease. | 2006 | 16478511 |
| sterilizing bacillus pumilus spores using supercritical carbon dioxide. | supercritical carbon dioxide (sc co(2)) has been evaluated as a new sterilization technology. results are presented on killing of b. pumilus spores using sc co(2) containing trace levels of additives. complete killing was achieved with 200 part per million (ppm) hydrogen peroxide in sc co(2) at 60 degrees c, 27.5 mpa. addition of water to sc co(2) resulted in greater than three-log killing, but this is insufficient to claim sterilization. neither ethanol nor isopropanol when added to sc co(2) af ... | 2006 | 16516991 |
| isolation and study of a bacteriophage for bacillus pumilus. | 1960 | 16561865 | |
| selection and identification of bacteria isolated from waste crude oil with polycyclic aromatic hydrocarbons removal capacities. | fifteen bacterial strains isolated from solid waste oil samples were selected due to their capacity of growing in the presence of hydrocarbons. the isolates were identified by pcr of the 16s rdna gene using fd1 and rd1 primers. the majority of the strains belonged to genera bacillus, bacillus pumilus (eight strains) and bacillus subtilis (two strains). besides, three strains were identified as micrococcus luteus, one as alcaligenes faecalis and one strain as enterobacter sp. growth of the above- ... | 2006 | 16564960 |
| xylose utilization and short-chain fatty acid production by selected components of the intestinal microflora of a rodent pollinator (aethomys namaquensis). | namaqua rock mice (aethomys namaquensis) consume nectar xylose when visiting protea flowers. whole-animal metabolism studies suggest that the gastrointestinal microflora plays an important role in xylose metabolism in a. namaquensis. we collected caecal contents under anaerobic conditions, cultured caecal microflora both aerobically and anaerobically, and assessed caecal microbial xylose utilization using a (14)c-xylose incubation assay. all four mice sampled hosted culturable caecal micro-organ ... | 2006 | 16676189 |
| search for antibacterial and antifungal agents from selected indian medicinal plants. | a series of 61 indian medicinal plants belonging to 33 different families used in various infectious disorders, were screened for their antimicrobial properties. screening was carried out at 1000 and 500 microg/ml concentrations by agar dilution method against bacillus cereus var mycoides, bacillus pumilus, bacillus subtilis, bordetella bronchiseptica, micrococcus luteus, staphylococcus aureus, staphylococcus epidermidis, escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa, streptoco ... | 2006 | 16678369 |
| production of indole-3-acetic acid in the plant-beneficial strain pseudomonas chlororaphis o6 is negatively regulated by the global sensor kinase gacs. | certain plant growth-promoting bacteria, such as pseudomonas fluorescens 89b61 and bacillus pumilus se34, secreted high levels of indole-3-acetic acid (iaa) in tryptophan-amended medium in stationary phase as determined by chromogenic analysis and high-performance liquid chromatography. two other growth-promoting strains, p. chlororaphis o6 and serratia marcescens 90-166, did not produce these high levels of iaa. however, when the gacs mutant of p. chlororaphis o6 was grown in tryptophan-supplem ... | 2006 | 16732458 |
| functional evaluation of a novel constitutive promoter f1 of bacillus pumilus, as a rice epiphytic strain, and construction of an efficient expression and secretion system under the control of f1. | to establish a constitutive, high-efficiency expression and secretion system for bacillus pumilus, the function of a promoter and the abilities of three signal peptides in b. pumilus dx01 were tested. f1, cloned from the rice epiphyte b. pumilus strain dx01, had strong transcription activity and was a vegetative-phase constitutive promoter. the signal sequences of bacillus subtilis levansucrase (sacb) and subtilisin, as well as b. pumilus dx01 rnase signal sequence could drive the secretion of e ... | 2006 | 16799766 |
| use of protoplasts from paired heterogenic bacterial species to detect tin contaminants: prospects for biosensor development. | two different bacteria gave different respiratory responses to the test analytes, tributyl tin (tbt) and cadmium as expressed by positive sigmoid responses by halomonas sp. (slope, +1.71 [tbt]; +1.76 [cd]) and negative sigmoid responses by bacillus pumilis (slope, -1.06 [tbt]; -0.59 [cd]). the ec50 values determined from hill plots for the response of halomonas sp. to the tbt and cd were 1 and 8.5 mm, respectively, which were lower by a factor of 10 than the corresponding values for b. pumilis. ... | 2007 | 16815699 |
| bacillus aerius sp. nov., bacillus aerophilus sp. nov., bacillus stratosphericus sp. nov. and bacillus altitudinis sp. nov., isolated from cryogenic tubes used for collecting air samples from high altitudes. | four novel bacterial strains were isolated from cryogenic tubes used to collect air samples at altitudes of 24, 28 and 41 km. the four strains, 24k(t), 28k(t), 41kf2a(t) and 41kf2b(t), were identified as members of the genus bacillus. phylogenetic analysis based on 16s rrna gene sequences indicated that three of the strains, 24k(t), 28k(t) and 41kf2a(t), are very similar to one another (>98 % sequence similarity) and show a similarity of 98-99 % with bacillus licheniformis and 98 % with bacillus ... | 2006 | 16825614 |
| production of cellulase-free endoxylanase from novel alkalophilic thermotolerent bacillus pumilus by solid-state fermentation and its application in wastepaper recycling. | the present study aimed at optimization of culture condition for the enhanced production of extra cellular thermostable cellulase-free xylanase from bacillus pumilus by solid-state fermentation. batch studies were carried out to evaluate various agro-industrial residues such as rice bran, rice husk, rice straw, sawdust, coconut pith, sugarcane bagasse and wheat bran for enzyme production by the bacterial culture. the endoxylanase production was highest on wheat bran media (5582 u/gds), which was ... | 2007 | 16844369 |
| bacillus spp. among hospitalized patients with haematological malignancies: clinical features, epidemics and outcomes. | between april 2000 and may 2005, 350 bacteraemic episodes occurred among patients treated in our haematology unit. two hundred and twenty-eight of these episodes were caused by gram-positive pathogens, most commonly coagulase-negative staphylococci and staphylococcus aureus. one hundred and twenty-two episodes were due to gram-negative pathogens, with a predominance of escherichia coli, acinetobacter baumannii and pseudomonas aeruginosa. bacillus bacteraemias constituted 12 of these episodes occ ... | 2006 | 16891037 |
| bacillus safensis sp. nov., isolated from spacecraft and assembly-facility surfaces. | thirteen strains of a novel spore-forming, gram-positive, mesophilic heterotrophic bacterium were isolated from spacecraft surfaces (mars odyssey orbiter) and assembly-facility surfaces at the jet propulsion laboratory in california and the kennedy space center in florida. phylogenetic analysis of 16s rrna gene sequences has placed these novel isolates within the genus bacillus, the greatest sequence similarity (99.9 %) being found with bacillus pumilus. however, these isolates share a mere 91.2 ... | 2006 | 16902000 |
| identification and characterization of a gamma-glutamyl transpeptidase from a thermo-alcalophile strain of bacillus pumilus. | a gamma-glutamyltranspeptidase (ggt, e.c. 2.3.2.2) was isolated from a strain (a8) originating from lake bogoria (kenya) and homologous with bacillus pumilus. this ggt shows an optimal activity at ph 8.9 and 62 degrees c. the enzyme is thermostable up to 43 degrees c. the best reagent among the potential inhibitors was shown to be don, which is an inhibitor highly specific for ggts. gly-gly-ala, gly-gly-gly and gly-gly were identified as the best acceptors for the transpeptidation reactions cata ... | 2006 | 17043758 |
| development of a bacteriophage model system to investigate virus inactivation methods used in the treatment of bone allografts. | bone allografts are commonly used in a variety of surgical procedures, to reconstruct lost bone stock and to provide mechanical support during the healing process. due to concerns regarding the possibility of disease transmission from donor to recipient, and of contamination of grafts during retrieval and processing procedures, it is common practice to sterilise bone allografts prior to issue for clinical use. it is vital that the sterilisation processes applied to allografts are validated to de ... | 2007 | 17061148 |
| small rolling circle plasmids in bacillus subtilis and related species: organization, distribution, and their possible role in host physiology. | bacillus subtilis and related species (bacillus licheniformis, bacillus pumilus, bacillus amyloliquefaciens, and bacillus mojavensis) represent a group of bacteria largely studied and widely employed by industry. small rolling circle replicating plasmids of this group of bacteria have been intensively studied as they represent a convenient model for genetic research and for the construction of molecular tools for the genetic modification of their hosts. through the computational analysis of the ... | 2007 | 17064773 |
| [the role of the promoter structure in the efficiency of the expression of guanylspecific ribonucleases from bacillus intermedius and bacillus pumilus]. | guanylspecific ribonucleases from b. intermedius (binase) and b.pumilus (rnase bpu) are structural and functional homologues, and their biosynthesis is subjected to the same laws. at the same time, there are essential differences in the expression efficiency of binase and rnase bpu genes. this was first suggested to be due to differences in nucleotide sequences of promoters of the genes. therefore, we constructed plasmids changing each different nucleotide in binase promoter for corresponding on ... | 2006 | 17094653 |
| [construction of an inducible and efficient expression-secretion shuttle vector of b. subtilis]. | a new shuttle vector psb which could replicate in both escherichia coli and bacillus subtilis was constructed by fusing the e. coli plasmid psp72 with the b. subtilis plasmid pub18. after the sacb promoter and signal peptide sequence of b. subtilis was inserted in the multiple cloning sites (mcs) of psb, the recombinant plasmid was designated as psbp. the alpha-amylase gene terminator of bacillus licheniformis was inserted at the other end of the mcs, resulting in expression-secretion plasmid ps ... | 2006 | 17172015 |
| bismuth-norfloxacin complex: synthesis, physicochemical and antimicrobial evaluation. | norfloxacin is a fluoroquinolone antibacterial agent which is active against various gram-positive as well as gram-negative microorganisms. presence of metal ions considerably alters the activity of fluoroquinolones against potentially susceptible bacteria. as bismuth is known to possess a good antibacterial activity, bismuth complex of norfloxacin was prepared by reacting bismuth citrate with aqueous solution of norfloxacin. the structure of the bismuth-norfloxacin complex (bnc) was confirmed b ... | 2007 | 17174046 |
| microbiological safety and clinical efficacy of radiation sterilized amniotic membranes for treatment of second-degree burns. | amniotic membranes collected from the placentae of screened donors were processed and sterilized by gamma irradiation at 25 kgy. the sterility assurance level (sal) of gamma irradiated amniotic membranes and clinical efficacy in second-degree burn wound healing were evaluated. processed air-dried amniotic tissue from 159 batches of processing was checked for the bioburden level before sterilization. about 39% of the tissues had bioburden in the range of 10(1)-10(2)/100 cm(2) and 54.8% in the ran ... | 2007 | 17182190 |
| screening and mutagenesis of a novel bacillus pumilus strain producing alkaline protease for dehairing. | to characterize and optimize a novel bacillus pumilus strain isolated from biological waste which produces protease with excellent dehairing effect. this newly isolated strain could be utilized in the industrial leather dehairing process. | 2007 | 17209806 |
| cutaneous infection due to bacillus pumilus: report of 3 cases. | human infection due to bacillus pumilus is exceptional. we report 3 cases of cutaneous infection caused by b. pumilus that occurred in 3 shepherds, 2 of whom were members of the same family. the lesions appeared to have a morphology similar to that of cutaneous anthrax lesions. two patients were cured after treatment with amoxicillin-clavulanate, and the third patient was cured after prolonged treatment with ciprofloxacin. to our knowledge, primary cutaneous infection due to b. pumilus has not b ... | 2007 | 17243047 |
| food poisoning associated with pumilacidin-producing bacillus pumilus in rice. | food poisoning caused by other bacillus species than b. cereus has been described, but the toxins involved have rarely been isolated. endospores will survive heat treatment and will germinate and multiply in cooked foods producing toxins under appropriate conditions. we describe a small food poisoning outbreak where three people became ill after a dinner in a chinese restaurant. acute symptoms including dizziness, headache, chills and back pain developed during the meal, and a few hours later th ... | 2007 | 17275116 |
| characterization of antimicrobial compounds from a common fern, pteris biaurita. | methanol extract was prepared from the fronds of pteris biaurita and partial purification was done by solvent partitioning with diethyl ether and ethyl acetate, followed by hydrolysis and further partitioning with ethyl acetate. the three fractions, thus obtained were bioassayed separately against five test fungi--curvularia lunata, fomes lamaoensis, poria hypobrumea, fuasrium oxysporum and a bacterium--bacillus pumilus, by spore germination, radial growth and agar cup techniques. results reveal ... | 2007 | 17373375 |
| antimicrobial activity of bacillus subtilis and bacillus pumilus during the fermentation of african locust bean (parkia biglobosa) for soumbala production. | to examine predominant isolates of bacillus subtilis and b. pumilus isolated from soumbala for their antimicrobial activity against indicator microorganisms as micrococcus luteus, staphyloccocus aureus, bacillus cereus, enterococus facium, listeria monocytogenes, escherichia coli, salmonella typhimurium, shigella dysenteriae, yersinia enterocolitica, aspergillus ochraceus and penicillium roqueforti. | 2007 | 17381739 |
| clostridium butyricum to-a culture supernatant downregulates tlr4 in human colonic epithelial cells. | the present study was performed to examine whether probiotics affect toll-like receptor 4 (tlr4) expression in human colonic epithelial cells. culture supernatants or heat-killed bacteria of bacillus mesentericus to-a, clostridium butyricum to-a, and streptococcus faecalis t-110 were applied to human colonic epithelial cells. treatment with c. butyricum to-a culture supernatant significantly reduced tlr4 mrna level (x0.16), even in the presence of interferon-gamma (ifn-gamma; x0.21) as compared ... | 2007 | 17404865 |
| degradation of bisphenol a by bacillus pumilus isolated from kimchi, a traditionally fermented food. | novel bisphenol a (bpa)-degrading bacterial strains, designated as bp-2ck, bp-21dk, and bp-22dk, were isolated from kimchi, a traditionally fermented food. these isolates were identified as bacillus pumilus and efficiently degraded bpa in a medium supplemented with nutrients such as peptone, beef extract, and yeast extract. strains bp-2ck, bp-21dk, and bp-22dk successfully degraded 25, 25, and 50 ppm of bpa, respectively, and all strains exhibited bpa-degrading activity in the presence of 10% na ... | 2007 | 17416976 |
| cytotoxicity in bacillus mojavensis is abolished following loss of surfactin synthesis: implications for assessment of toxicity and food poisoning potential. | bacillus subtilis and the closely related species bacillus pumilus and bacillus licheniformis have periodically been suggested to play a role in the aetiology of food poisoning despite the fact that the organisms do not possess the genes associated with enteropathogenicity in bacillus cereus. we show here that bacillus mojavensis, an organism closely related to b. subtilis, is able to produce toxic components which identify as a complex of three different surfactin analogues. these cyclic lipope ... | 2007 | 17467096 |
| a new organic solvent tolerant protease from bacillus pumilus 115b. | five out of the nine benzene-toulene-ethylbenzene-xylene (btex) tolerant bacteria that demonstrated high protease activity on skim milk agar were isolated. among them, isolate 115b identified as bacillus pumilus exhibited the highest protease production. the protease produced was stable in 25% (v/v) benzene and toluene and it was activated 1.7 and 2.5- fold by n-dodecane and n-tetradecane, respectively. the gene encoding the organic solvent tolerant protease was cloned and its nucleotide sequenc ... | 2007 | 17492323 |
| biotransformation of isoeugenol to vanillin by a newly isolated bacillus pumilus strain: identification of major metabolites. | a bacterial strain s-1 capable of transforming isoeugenol to vanillin was isolated. the strain was identified as bacillus pumilus based on biochemical tests, cellular fatty acid composition, riboprint pattern and 16s rrna gene sequence analyses. in the biotransformation of isoeugenol, vanillin was the main product. with the growing culture of b. pumilus s-1, 10g l(-1) isoeugenol was converted to 3.75g l(-1) vanillin in 150 h, with a molar yield of 40.5% that is the highest up to now. dehydrodiis ... | 2007 | 17583367 |
| comparison of four antibiotics with indigenous marine bacillus spp. in controlling pathogenic bacteria from shrimp and artemia. | use of antibiotics for the control of bacterial diseases in shrimp culture has caused several adverse impacts to the industry. this has resulted in the search for alternative environment friendly approaches to overcome bacterial infections. this study was conducted to investigate the use of beneficial bacteria as an alternative to antibiotics. ten pathogenic bacterial species isolated from shrimp, penaeus monodon, and artemia cysts were tested for susceptibility to indigenous marine bacillus sub ... | 2007 | 17584435 |
| probiotics reduce infectious complications after pancreaticoduodenectomy. | postoperative morbidity is a significant problem associated with pancreaticoduodenectomy. the clinical value of probiotics in surgical patients remains unclear. this study investigated the effect of probiotics on surgical outcome after pancreaticoduodenectomy. | 2007 | 17591036 |
| toxinogenic bacillus pumilus and bacillus licheniformis from mastitic milk. | to elucidate the occurrence of heat-stable toxin-producing strains among mastitic bacillus isolates, 100 milk samples of mastitic cows from different parts of finland were screened. bacillus was identified as the major organism in 23 samples. toxinogenic bacillus isolates identified by sperm cell motility inhibition assay were isolated from six samples. four isolates belonged to the species bacillus pumilus and two to bacillus licheniformis. the toxic substances were heat-stable and soluble to m ... | 2007 | 17611049 |
| [conditions of the biosynthesis of an extracellular subtilisin-like proteinase by bacillus pumilus kmm 62]. | the influence of the cultivation conditions on bacillus pumilus kmm 62 growth and effectiveness of the production of a subtilisin-like serine proteinase were investigated. enzyme accumulation in the culture fluid reached the maximum value after 32 and 46-48 h of growth; it depends on the composition of the nutrient medium. the ratio of the concentrations of two main components of the medium, peptone and inorganic phosphate, which was optimal for enzyme biosynthesis was determined by multifactor ... | 2007 | 17633406 |
| [cloning, characterization and application of the promoter of alkaline protease gene in bacillus pumilus]. | a 797 bp promoter fragment of alkaline protease gene was cloned from the bacillus pumilus genome by employing tail-pcr strategy. the sequence analysis of this promoter fragment showed that the sequence accounting for gene expression was approximately 390bp. deletion analysis of the fragment defined the minimal required sequence of promoter for initiating transcription lies on a 160 bp region upstream of the start codon. the alkaline protease gene wapq3 containing the cloned promoter fragment was ... | 2007 | 17646155 |
| a colorimetric assay for the determination of acetyl xylan esterase or cephalosporin c acetyl esterase activities using 7-amino cephalosporanic acid, cephalosporin c, or acetylated xylan as substrate. | a bromothymol blue-based colorimetric assay has been devised to screen for acetyl xylan esterase or cephalosporin c (cpc) deacetylase activities using 7-amino cephalosporanic acid (7-aca), cpc, or acetylated xylan as substrate. these enzymes are not screened with their natural substrates because of the tedious procedures available previously. acetyl xylan esterase from bacillus pumilus cect 5072 was cloned, expressed in escherichia coli rosetta (de3), and characterized using this assay. similar ... | 2007 | 17651681 |
| endophytic bacteria in sunflower (helianthus annuus l.): isolation, characterization, and production of jasmonates and abscisic acid in culture medium. | this study was designed to isolate and characterize endophytic bacteria from sunflower (helianthus annuus) grown under irrigation and water stress (drought) conditions, to analyze growth of isolated bacteria under drought condition, and to evaluate the ability of bacteria isolated from plants cultivated under drought to produce jasmonates (jas) and abscisic acid (aba). bacteria were isolated from soil samples collected when sunflower plants were at the end of the vegetative stage. a total of 29 ... | 2007 | 17657487 |
| pumilicin 4, a novel bacteriocin with anti-mrsa and anti-vre activity produced by newly isolated bacteria bacillus pumilus strain wapb4. | a total of 34 bacterial strains with anti-methicillin-resistant staphylococcus aureus (mrsa) activity were isolated from 69 soil and water samples collected from four areas of thailand. one strain, wapb4 identified as bacillus pumilus, showed remarkable antibacterial activity against mrsa, vancomycin-resistant enterococcus faecalis (vre), and several gram-positive test bacteria. bacteriocin produced by wapb4 was designated as pumilicin 4. it was heat stable up to 121 degrees c, 15 min and active ... | 2007 | 17700984 |
| production of thermostable pectinase and xylanase for their potential application in bleaching of kraft pulp. | a very high level of alkalophilic and thermostable pectinase and xylanase has been produced from newly isolated strains of bacillus subtilis and bacillus pumilus respectively. enzyme production for pectinase was carried out under ssf using combinations of cheap agricultural residues while xylanase was produced under submerged fermentation using wheat bran as substrate to minimize the cost of production of these enzymes among the various substrates tested, the highest yield of pectinase productio ... | 2007 | 17726619 |
| [molecular docking of bacillus pumilus xylanase and xylan substrate using computer modeling]. | bacillus pumilus xylanase was cloned and sequenced. based on the tertiary structure that originated from homology modeling, the potential active pocket was searched and ligand-protein docking was performed using relative softwares. the information extracted from the molecular docking is analyzed; several amino acid residues might play a vital role in the xylanase catalytic reaction are obtained to instruct the further modification of xylanase directed-evolution. | 2007 | 17822050 |
| antibacterial stilbenoids from the roots of stemona tuberosa. | twelve dihydrostilbenes, stilbostemins n-y (1-12), and a phenanthraquinone, stemanthraquinone (13), were isolated and identified from roots of stemona tuberosa, along with five known dihydrostilbenes. their structures were established on the basis of 1d and 2d nmr and other spectroscopic analyses. dihydrostilbene 8 exhibited strong activity against bacillus pumilus (mit 12.5-25 microg/ml). many tested compounds exhibited moderate antibacterial activities. | 2008 | 17826806 |
| paradoxical dna repair and peroxide resistance gene conservation in bacillus pumilus safr-032. | bacillus spores are notoriously resistant to unfavorable conditions such as uv radiation, gamma-radiation, h2o2, desiccation, chemical disinfection, or starvation. bacillus pumilus safr-032 survives standard decontamination procedures of the jet propulsion lab spacecraft assembly facility, and both spores and vegetative cells of this strain exhibit elevated resistance to uv radiation and h2o2 compared to other bacillus species. | 2007 | 17895969 |
| bacillus pumilus sg2 isolated from saline conditions produces and secretes two chitinases. | isolation and characterization of chitinases from a halotolerant bacillus pumilus. | 2007 | 17897213 |
| species-level identification of bacillus strains isolates from marine sediments by conventional biochemical, 16s rrna gene sequencing and inter-trna gene sequence lengths analysis. | the aim of this study was to compare the ability of commonly used conventional biochemical tests, sequencing analysis of 16s rrna genes and tdna-intergenic spacer length polymorphism (tdna-pcr) to identify species of the genus bacillus recovered from marine sediments. while biochemical tests were not sufficiently sensitive to distinguish between the 23 marine strains analyzed, partial 16s rrna gene sequences allowed a correct identification, clustering them into four species belonging to bacillu ... | 2008 | 17922298 |
| characterization of an alkaline active-thiol forming extracellular serine keratinase by the newly isolated bacillus pumilus. | the aim of the study was to optimize microbial degradation of keratinous waste and to characterize the alkaline active keratinase showing its biotechnological importance. | 2008 | 17922821 |
| central venous catheter infection with bacillus pumilus in an immunocompetent child: a case report. | bacillus organisms are common laboratory contaminants. the majority of bacillus bacteraemias are transient and not clinically significant. clinically significant infection due to bacillus species is rare and mostly due to bacillus cereus infections in immuno-compromised hosts. | 2007 | 17967173 |
| nutritional regulation of keratinolytic activity in bacillus pumilis. | bacillus pumilis f3-4 utilized feather as a sole source of carbon, nitrogen and sulfur. supplementation of the feather medium with glucose or mgso(4) . 7h(2)o increased keratinolytic protease production (14.6-16.7 u/mg). the synthesis of keratinolytic protease was repressed by an exogenous nitrogen source. keratinolytic protease was produced in the absence of feather (9.4 u/mg). feather degradation resulted in sulfhydryl group formation (0.8-2.6 microm). b. pumilis f3-4 effectively degraded chic ... | 2008 | 17968508 |
| isolation and assessment of phytate-hydrolysing bacteria from the delmarva peninsula. | the delaware-maryland-virginia (delmarva) peninsula, flanking one side of the chesapeake bay, is home to a substantial broiler chicken industry. as such, it produces a significant amount of manure that is typically composted and spread onto local croplands as a fertilizer. phytate (myo inositol hexakisphosphate), the major form of organic phosphorus in the manure, can be hydrolysed by microorganisms to produce orthophosphate. orthophosphate is a eutrophication agent which can lead to algal bloom ... | 2007 | 17991037 |
| immobilization of xylanase from bacillus pumilus strain mk001 and its application in production of xylo-oligosaccharides. | xylanase from bacillus pumilus strain mk001 was immobilized on different matrices following varied immobilization methods. entrapment using gelatin (ge) (40.0%), physical adsorption on chitin (ch) (35.0%), ionic binding with q-sepharose (q-s) (45.0%), and covalent binding with hp-20 beads (42.0%) showed the maximum xylanase immobilization efficiency. the optimum ph of immobilized xylanase shifted up to 1.0 unit (ph 7.0) as compared to free enzyme (ph 6.0). the immobilized xylanase exhibited high ... | 2007 | 18025574 |
| [the effect of spo0a and abrb proteins on expression of the genes of guanyl-specific ribonucleases from bacillus intermedius and bacillus pumilus in bacillus subtilis recombinant strains]. | guanyl-specific ribonucleases from bacillus intermedius and bacillus pumilus are actively secreted under phosphate starvation by recombinant strains of bacillus subtilis with native regulatory systems and by strains defective in some proteins of the spo0a phosphorylation pathway. the level of expression of ribonuclease genes has been shown to increase approximately sixfold in recombinant strains with mutation in the spo0a gene and threefold in the spo0a/abrb mutants, as compared with native stra ... | 2007 | 18069324 |
| purification and characterization of an extracellular alpha-l-arabinosidase from a novel isolate bacillus pumilus ara and its over-expression in escherichia coli. | the alpha-l-arabinosidase, arab, was induced when bacillus pumilus ara was grown at 50 degrees c in a minimal medium containing xylan. a 56-kda protein with alpha-l-arabinosidase activity was purified from culture supernatant to gel electrophoretic homogeneity. the optimal activity was at ph 6.4 and 60 degrees c over a 10-min assay. the purified enzyme was stable over a ph range of 5.2-7.6 and had a 1-h half life at 70 degrees c. the enzyme released arabinose from oat spelt xylan. kinetic experi ... | 2008 | 18071642 |
| effect of shadowing on survival of bacteria under conditions simulating the martian atmosphere and uv radiation. | spacecraft-associated spores and four non-spore-forming bacterial isolates were prepared in atacama desert soil suspensions and tested both in solution and in a desiccated state to elucidate the shadowing effect of soil particulates on bacterial survival under simulated martian atmospheric and uv irradiation conditions. all non-spore-forming cells that were prepared in nutrient-depleted, 0.2-microm-filtered desert soil (dse) microcosms and desiccated for 75 days on aluminum died, whereas cells p ... | 2008 | 18083857 |
| an antibiotic from bacillus mesentericus. | 1948 | 18108412 | |
| in vitro inhibitory activity of probiotic spore-forming bacilli against genotoxins. | to investigate the ability of bacilli of various species (bacillus clausii, bacillus subtilis, bacillus lentus, bacillus pumilus. bacillus megaterium, bacillus firmus, bacillus sp.) and origins (probiotic and collection strains) to counteract the activity of some representative dna-reactive agents. | 2008 | 18194161 |
| biodegradation of phenanthrene and pyrene from mangrove sediment in subtropical taiwan. | this study investigated the effects of various factors on the biodegradation of the polycyclic aromatic hydrocarbons (pahs) phenanthrene and pyrene, and characterized the structure of the microbial community in mangrove sediment from subtropical taiwan. the degradation of pah was enhanced by the addition of brij 30, tween 80, yeast extract, hydrogen peroxide, sodium chloride or cellulose. however, pah degradation was inhibited by the addition of humic acid, di-(2-ethylhexyl), phthalate (dehp) or ... | 2008 | 18205053 |
| isolation of 8'-phosphate ester derivatives of amicoumacins: structure-activity relationship of hydroxy amino acid moiety. | two new compounds, 8'-phospho derivatives of amicoumacins a and b, were isolated from the culture broth of a strain of bacillus pumilus together with amicoumacins a and b. their structures were elucidated on the basis of spectroscopic methods and alkaline phosphatase treatments. comparison of the antibacterial activities against methicillin-resistant staphylococcus aureus (mrsa) of these compounds suggested that c-8' hydroxyl and c-12' amide group of amicoumacin a played a critical role for anti ... | 2007 | 18277000 |
| detoxification of toxic heavy metals by marine bacteria highly resistant to mercury. | pollution in industrial areas is a serious environmental concern, and interest in bacterial resistance to heavy metals is of practical significance. mercury (hg), cadmium (cd), and lead (pb) are known to cause damage to living organisms, including human beings. several marine bacteria highly resistant to mercury (bhrm) capable of growing at 25 ppm (mg l(-1)) or higher concentrations of mercury were tested during this study to evaluate their potential to detoxify cd and pb. results indicate their ... | 2008 | 18288535 |
| radiation sterilisation of doxorubicin bound to poly(butyl cyanoacrylate) nanoparticles. | doxorubicin-loaded poly(butyl cyanoacrylate) (pbca) nanoparticles were prepared by anionic polymerisation under non-aseptic conditions. the feasibility of sterilisation of this formulation using either gamma-irradiation or electron beam irradiation was investigated. the irradiation doses ranged from 10 to 35 kgy. bacillus pumilus was used as the official test microorganism. the bioburden of the untreated formulation was found to be 100 cfu/g. microbiological monitoring revealed that at this leve ... | 2008 | 18295418 |
| experimental and qsar studies on antimicrobial activity of benzimidazole derivatives. | twenty eight analogues of benzimidazoles had been synthesized and tested for their antimicrobial activity against four bacteria (staphylococcus auerus, escherichia coli, bacillus pumilus and proteus vulgaris) and two fungi (aspergillus flavus and aspergilus niger). compounds with r as c6h4no2 and r' as so2c6h4-ch3(p), with r as c6h4och3 and r' as so2c6h4-ch3(p), and with r as ch2c6h5 and r' as ch2(ch2)9cl exhibited comparable or higher antibacterial activity than ciprofloxacin against s. auerus ... | 2008 | 18310935 |
| a novel family of antimicrobial peptides from the skin of amolops loloensis. | while conducting experiments to investigate antimicrobial peptides of amphibians living in the yunnan-sichuan region of southwest china, a new family of antimicrobial peptides was identified from skin secretions of the rufous-spotted torrent frog, amolops loloensis. members of the new peptide family named amolopins are composed of 18 amino acids with a unique sequence, for example, nilssivnginralsffg. by blast search, amolopins did no show similarity to any known peptides. among the tested micro ... | 2008 | 18312859 |
| action of antimicrobial substances produced by different oil reservoir bacillus strains against biofilm formation. | microbial colonization of petroleum industry systems takes place through the formation of biofilms, and can result in biodeterioration of the metal surfaces. in a previous study, two oil reservoir bacillus strains (bacillus licheniformis t6-5 and bacillus firmus h(2)o-1) were shown to produce antimicrobial substances (ams) active against different bacillus strains and a consortium of sulfate-reducing bacteria (srb) on solid medium. however, neither their ability to form biofilms nor the effect o ... | 2008 | 18330565 |
| biochemical and molecular characterization of a detergent-stable serine alkaline protease from bacillus pumilus cbs with high catalytic efficiency. | we have described previously the potential use of an alkaline protease from bacillus pumilus cbs as an effective additive in laundry detergent formulations [b. jaouadi, s. ellouz-chaabouni, m. ben ali, e. ben messaoud, b. naili, a. dhouib, s. bejar, a novel alkaline protease from bacillus pumilus cbs having a high compatibility with laundry detergent and a high feather-degrading activity, process biochem, submitted for publication]. here, we purified this enzyme (named sapb) and we cloned, seque ... | 2008 | 18397761 |
| comparative analysis of the diversity of aerobic spore-forming bacteria in raw milk from organic and conventional dairy farms. | bacterial contamination of raw milk can originate from different sources: air, milking equipment, feed, soil, faeces and grass. it is hypothesized that differences in feeding and housing strategies of cows may influence the microbial quality of milk. this assumption was investigated through comparison of the aerobic spore-forming flora in milk from organic and conventional dairy farms. laboratory pasteurized milk samples from five conventional and five organic dairy farms, sampled in late summer ... | 2008 | 18406093 |
| extracellular proteolytic activities expressed by bacillus pumilus isolated from endodontic and periodontal lesions. | the purpose of the present study was to identify 12 bacillus isolates that had been obtained from root canals of teeth requiring endodontic therapy and from periodontal pockets in severe marginal periodontitis, and to determine whether these isolates exhibited extracellular proteolytic activity and, using in vitro assays, whether any such activity could degrade substrates that would be pathophysiologically relevant with regard to the production of endodontic and periodontal lesions. biochemical ... | 2008 | 18436599 |
| thermodynamic characterization of a highly thermoactive extracellular pectate lyase from a new isolate bacillus pumilus dks1. | an extracellular pectate lyase (ec 4.2.2.2) was purified from the culture filtrate of a newly isolated bacillus pumilus dks1 grown in pectin containing medium. using ion-exchange and gel filtration chromatography, this enzyme was purified and found to have a molecular weight of around 35kda. the purified enzyme exhibited maximal activity at a temperature of 75 degrees c and ph 8.5. the presence of 1mm calcium and manganese enhanced pectate lyase activity and was strongly inhibited by zinc, nicke ... | 2008 | 18445524 |
| enterotoxins and emetic toxins production by bacillus cereus and other species of bacillus isolated from soumbala and bikalga, african alkaline fermented food condiments. | the ability of various species of bacillus from fermented seeds of parkia biglobosa known as african locust bean (soumbala) and fermented seeds of hibiscus sabdariffa (bikalga) was investigated. the study included screening of the isolates by haemolysis on blood agar, detection of toxins in broth and during the fermentation of african locust bean using the bacillus cereus enterotoxin reverse passive latex agglutination test kit (bcet-rpla) and the bacillus diarrhoeal enterotoxin visual immunoass ... | 2008 | 18474404 |
| ethanol/water pulps from sugar cane straw and their biobleaching with xylanase from bacillus pumilus. | the influence of independent variables (temperature and time) on the cooking of sugar cane straw with ethanol/water mixtures was studied to determine operating conditions that obtain pulp with high cellulose contents and a low lignin content. an experimental 2(2) design was applied for temperatures of 185 and 215 degrees c, and time of 1 and 2.5 h with the ethanol/water mixture concentration and constant straw-to-solvent ratio. the system was scaled-up at 200 degrees c cooking temperature for 2 ... | 2007 | 18478412 |
| synthesis and antibacterial activity of some new 2,3-dimethoxy-3-hydroxy-2-(1-phenyl-3-aryl-4-pyrazolyl)chromanones. | seven new 2,3-dimethoxy-3-hydroxy-2-(1-phenyl-3-aryl-4-pyrazolyl)chromanones (5) have been synthesized by the oxidation of 3-hydroxy-2-(1-phenyl-3-aryl-4-pyrazolyl)chromones (4) with iodobenzene diacetate in methanol. the structures of compounds 5 were established by the combined use of (1)h nmr, ir and mass spectra. all the seven compounds (5) were tested in vitro for their antibacterial activity against gram-positive bacteria namely, staphylococcus aureus, staphylococcus epidermidis and bacill ... | 2009 | 18504062 |