Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| the specific genes for lantibiotic mutacin ii biosynthesis in streptococcus mutans t8 are clustered and can be transferred en bloc. | mutacin ii is a ribosomally synthesized peptide lantibiotic produced by group ii streptococcus mutans. dna sequencing has revealed that the mutacin ii biosynthetic gene cluster consists of seven specific open reading frames: a regulator (mutr), the prepromutacin structural gene (muta), a modifying protein (mutm), an abc transporter (mutt), and an immunity cluster (mutfeg). transformations of a non-mutacin-producing strain, s. mutans ua159, and a mutacin i-producing strain, s. mutans ua140, with ... | 1999 | 10049909 |
| antibacterial synergistic effect of chlorhexidine and hydrogen peroxide against streptococcus sobrinus, streptococcus faecalis and staphylococcus aureus. | chlorhexidine (chx) and hydrogen peroxide (hp) are potent antibacterial agents that are used in controlling dental plaque. however, both agents bear undesired side-effects. we have tested the hypothesis that an antibacterial synergistic effect can occur between the two agents against streptococcus sobrinus, streptococcus faecalis and staphylococcus aureus. we have found that at several combinations of hp and chx an antibacterial synergistic effect does occur, while at other combinations a on-sig ... | 1999 | 10080313 |
| investigation of the role of human breast milk in caries development. | the objective of this study was to determine the caries-related risk associated with human breast milk (hbm). | 1999 | 10197331 |
| simultaneous induction of specific immunoglobulin a--producing cells in major and minor salivary glands after tonsillar application of antigen in rabbits. | the immunoglobulin a (iga)-producing cells in the stroma of major salivary glands are induced by antigenic stimulation of the mucosal immune system. whether such cells also are induced in minor salivary glands by this stimulation remains to be determined. after application of sheep red blood cells to the palatine tonsils every 3 days for 6 weeks, anti-sheep red blood cell iga was detected in saliva both by agglutination tests and by enzyme-linked immunosorbent assay. using enzyme-linked immunosp ... | 1999 | 10204476 |
| effect of matrix placement on furcation perforation repair. | furcation perforations are a serious complication during endodontic treatment, and a matrix to aid placement of repair material has been recommended. this study tested the sealing ability of amalgam and ketac silver placed with and without plaster of paris as a matrix. a bacterial penetration technique was used to test the seal. perforations created in the pulpal floor of extracted human mandibular molars were repaired as follows: group 1, amalgam; group 2, amalgam plus plaster as a matrix; grou ... | 1999 | 10321186 |
| topical antimicrobial therapy. | 1999 | 10355004 | |
| effect of oleanolic acid-cyclodextrin inclusion compounds on dental caries by in vitro experiment and rat-caries model. | earlier work in vitro showed that oleanolic acid (oa) was a potential inhibitor of insoluble glucan (isg) synthesis from mutans streptococci (ms). in this study, two oleanolic acid-cyclodextrin inclusion compounds (oa-cds), oleanolic acid-g1-beta-cyclodextrin (oa-g1-beta cd) and oleanolic acid-beta-cyclodextrin (oa-beta cd), were assayed for their effects on isg synthesis from streptococcus mutans mt8148r, and on the growth of oral bacteria. oa-beta cd inhibited isg synthesis by 55.3 and 37.4% a ... | 1999 | 10413873 |
| structure and enzymatic properties of genetically truncated forms of the water-insoluble glucan-synthesizing glucosyltransferase from streptococcus sobrinus. | glucosyltransferase-i (gtf-i: 175 kda) of a cariogenic bacterium, streptococcus sobrinus 6715, mediates the conversion of water-soluble dextran (alpha-1,6-glucan) into a water-insoluble form by making numerous alpha-1,3-glucan branches along the dextran chains with sucrose as the glucosyl donor. the structures and catalytic properties were compared for two gtf-i fragments, gtf-i' (138 kda) and gs (110 kda). both lack the n-terminal 84 residues of gtf-i. while gtf-i' still contains four of the si ... | 1999 | 10423519 |
| recolonisation pattern of mutans streptococci after suppression by three different modes of chlorhexidine gel application. | clinical studies have shown conflicting results for the reduction of approximal caries when chlorhexidine gel has been applied either in trays or by flossing. to investigate whether these modes of treatment affect the colonization of mutans streptococci (ms) differently, we quantified ms in saliva and on various tooth surfaces before and after a single chlorhexidine gel application. twenty-one schoolchildren (15-16 yr old), with high numbers of salivary ms, were randomised into three groups. the ... | 1999 | 10424380 |
| a new mechanism of action of fluoride on streptococci. | addition of fluoride to the growth medium of streptococcus sobrinus resulted in a loss of glucan-binding lectin activity. upon removal of fluoride, the bacteria regained their ability to bind glucan in about one generation. chloramphenicol prevented recovery of ability to produce the lectin, showing the requirement for protein synthesis. fluoride also caused a significant reduction in the tendency of the streptococci to form chains of cells, although the spent medium from fluoride-containing gro ... | 1999 | 10434061 |
| effect of apis mellifera propolis from two brazilian regions on caries development in desalivated rats. | the purpose of the present study was to evaluate the effect of apis mellifera propolis collected from two regions of brazil on caries development in desalivated rats. ethanolic extracts of propolis (eep) were prepared from crude propolis samples collected in minas gerais state (mg), southeastern brazil, and rio grande do sul state (rs), southern brazil. the flavonoid composition of eep was analyzed by high-performance thin-layer chromatography (hptlc) and reversed-phase high-performance liquid c ... | 1999 | 10460964 |
| effects of bovine immune and non-immune whey preparations on the composition and ph response of human dental plaque. | colostral products from non-immunized cows (cp) and cows immunized with mutans streptococci (ip) were used as mouth rinses in a short-term human study. the acidogenic potential of the products was tested and found to be negligible in vivo before application to subsequent rinsing tests. at first, all the participants received a professional tooth cleaning, after which they rinsed with one of the solutions (ip; cp; water) three times per day for 3 d. after each rinsing period, the resting ph and d ... | 1999 | 10467939 |
| enhanced (+)-catechin transglucosylating activity of streptococcus mutans gs-5 glucosyltransferase-d due to fructose removal. | the (+)-catechin transglucosylating activities of several glucosyltransferases (gtfs) from the genus streptococcus were compared. for this purpose, a mixture of four gtfs from streptococcus sobrinus sl-1 and recombinant gtf-b and gtf-d from streptococcus mutans gs-5 expressed in escherichia coli were studied. it was shown that after removal of alpha-glucosidase activity, gtf-d transglucosylated catechin with the highest efficiency. a maximal yield (expressed as the ratio of moles of glucoside fo ... | 1999 | 10473427 |
| effect of salivary biofilm on the adherence of oral bacteria to bleached and non-bleached restorative material. | the objective of this work was to examine the effect of in vitro salivary biofilm on the adherence of oral bacteria to bleached and non-bleached restorative material (charisma). | 1999 | 10483391 |
| colostral proteins from cows immunised with streptococcus mutans/s. sobrinus support the phagocytosis and killing of mutans streptococci by human leucocytes. | passive immunisation, based on bovine colostral preparations, is an area of active research. specific bovine antibodies inhibit the virulence factors of target pathogens but the interactions between whey preparations and human immune defence cells are not well known. bovine colostrum inhibits the phagocytic activity of bovine leucocytes and this may reflect the biological activity of immunoglobulins in it. therefore, this study aimed to examine the effects of bovine whey protein preparations fro ... | 1999 | 10510968 |
| insoluble glucans synthesized by cariogenic streptococci: a structural study. | of the three cariogenic streptococci grown in four various culture media, the strain streptococcus mutans 20,381 was found to produce large amounts of extracellular glucosyltransferase and water-insoluble, adhesive exopolysaccharide when grown in batch culture on brain-heart infusion broth. methylation and nuclear magnetic resonance analyses revealed that the insoluble polymers synthesized by the crude glucosyltransferase preparations were mixed-linkage (1-->3), (1-->6)-alpha-d-glucans (so-calle ... | 1999 | 10520271 |
| powdered milk micellar casein prevents oral colonization by streptococcus sobrinus and dental caries in rats: a basis for the caries-protective effect of dairy products. | three animal studies were performed to investigate the influence of the macromolecular structure of milk casein on caries incidence and the possible ecological changes of the oral microbiota by such casein fractions. towards this end, rats were infected with mixed bacterial suspensions of streptococcus sobrinus omz 176 and actinomyces viscosus ny1. various milk protein fractions were incorporated into carefully balanced powdered cariogenic diets to constitute the sole major protein component. di ... | 1999 | 10529530 |
| studying biofilm formation of mutans streptococci. | 1999 | 10547815 | |
| a dna probe specific to streptococcus sobrinus. | three dna fragments (ssb-1, -2 and -3) in the dextranase gene (dex) of streptococcus sobrinus were amplified by polymerase chain reaction and used as dna probes. the probes were examined for the specificity and the sensitivity of hybridization with dna of oral streptococcal species. while probes ssb-1 and ssb-2 were specific to both s. sobrinus and streptococcus downei, ssb-3 was specific only to s. sobrinus. ssb-3 was able to detect 5 ng of chromosomal dna purified from s. sobrinus nidr6715 and ... | 1999 | 10551167 |
| effects of monoclonal antibody on colonization of streptococcus sobrinus and development of dental caries in rats. | to investigate the effect of local application of monoclonal antibody (mcab) raised against the 210kd cell surface protein antigen (pag) of streptococcus sobrinus on the colonization of rats' teeth and the development of dental caries. | 1999 | 10557177 |
| effects of topical application of free and liposome-encapsulated lactoferrin and lactoperoxidase on oral microbiota and dental caries in rats. | four groups of rats were inoculated with streptococcus sobrinus atcc 33478 and fed a cariogenic diet for 42 days. topical treatment with either distilled water, sodium fluoride (0.2%), a solution containing lactoferrin and lactoperoxidase, or a solution containing liposome-encapsulated lactoferrin and liposome-encapsulated lactoperoxidase was applied at intervals for 35 days. caries incidence in groups treated with liposome-encapsulated lactoferrin and lactoperoxidase was significantly lower tha ... | 1999 | 10580537 |
| anticaries effect of a component from shiitake (an edible mushroom). | the caries-inhibiting effect of the extract from shiitake (lentinus edodes), the most popular edible mushroom in japan, was studied both in vitro and in vivo. shiitake extract showed an inhibitory effect on water-insoluble glucan formation from sucrose by crude glucosyltransferases of streptococcus mutans jc-2 and streptococcus sobrinus omz-176. the firmly adherent plaque in the artificial plaque formation test was strongly inhibited by shiitake extract. the reduction of firmly adherent plaque c ... | 2000 | 10601791 |
| production, characterization, and application of monoclonal antibodies which distinguish four glucosyltransferases from streptococcus sobrinus. | a 1,3-alpha-glucan synthase (gtf-i), a highly branched 1, 6-alpha-glucan synthase (gtf-u) and a 1,6-alpha-glucan synthase (gtf-t) were purified to near homogeneity from the culture fluid of streptococcus sobrinus strain b13n (serotype d) and characterized. in addition, a crude preparation of a recombinant oligo-isomaltosaccharide synthase (rgtf-s) was prepared from a cell-free extract of escherichia coli md124 transformant. using four homogeneous gtf preparations including previously purified rg ... | 2000 | 10617784 |
| streptococcus sobrinus antigens that react to salivary antibodies induced by tonsillar application of formalin-killed s. sobrinus in rabbits. | we previously found that tonsillar application of antigen induces a strong antibody response to streptococcus sobrinus in saliva and blood plasma. rabbits immunized against s. sobrinus by tonsillar application were highly resistant to experimental dental caries triggered by oral inoculation of living s. sobrinus organisms with sucrose. in the present study, we examined the reaction of s. sobrinus antigens to the antibodies induced by the tonsillar application of s. sobrinus aht-k in rabbits and ... | 2000 | 10639439 |
| single-molecule imaging of interaction between dextran and glucosyltransferase from streptococcus sobrinus. | using total internal reflection fluorescence microscopy, we directly observed the interaction between dextran and glucosyltransferase i (gtf) of streptococcus sobrinus. tetramethylrhodamine (tmr)-labeled gtf molecules were individually imaged as they were associating with and then dissociating from the dextran fixed on the glass surface in the evanescent field. similarly dynamic behavior of tmr-labeled dextran molecules was also observed on the gtf-fixed surface. the duration of the stay on the ... | 2000 | 10648546 |
| development of multi-species consortia biofilms of oral bacteria as an enamel and root caries model system. | the aim was to establish defined-species consortium plaque biofilms to investigate enamel and root caries in an artificial mouth. strains of the putative enamel and root caries pathogens, streptococcus mutans, strep. sobrinus, actinomyces naeslundii and lactobacillus rhamnosus, were screened in batch culture for potential cariogenic properties: a low terminal ph, ability to aggregate, and catabolic diversity. the strains selected were grown as monoculture biofilms and as consortium plaque biofil ... | 2000 | 10669090 |
| influence of (s)-ketoprofen and fluoride on caries in rats. | non-steroidal anti-inflammatory agents (e.g., ketoprofen) used topically appear to be effective in reducing bone loss in the ligature model of periodontitis. ketoprofen, in common with some food preservatives, e.g., benzoate and sorbate, is a weak acid. fluoride, too, may behave as a weak acid and, similar to the other agents, may exert antibacterial effects. the purpose of the present study was to determine whether a combination of (s)-ketoprofen, an enantiomer of ketoprofen, alone or in combin ... | 2000 | 10673782 |
| signal sequence and alanine-rich region of streptococcal protein antigen a of streptococcus sobrinus can direct localization of alkaline phosphatase to the periplasm of escherichia coli. | streptococcal protein antigen a (spaa) of streptococcus sobrinus is expressed on the surface of cells and extracellularly. tnphoa which lacks signals for transcription and membrane transport of escherichia coli alkaline phosphatase was used to analyze the sequences necessary for transport of a spaa/phoa fusion protein across the cytoplasmic membrane to the periplasm of e. coli cells. of 15 alkaline phosphatase-producing isolates analyzed, all were found to localize more than 85% of the spaa/phoa ... | 2000 | 10689159 |
| in vitro antimicrobial activity of propolis and arnica montana against oral pathogens. | arnica and propolis have been used for thousands of years in folk medicine for several purposes. they possess several biological activities such as anti-inflammatory, antifungal, antiviral and tissue regenerative, among others. although the antibacterial activity of propolis has already been demonstrated, very few studies have been done on bacteria of clinical relevance in dentistry. also, the antimicrobial activity of arnica has not been extensively investigated. therefore the aim here was to e ... | 2000 | 10716618 |
| effects of caffeine and theophylline on the development of dental caries in rats. | the purpose of this study was to evaluate the influence of caffeine and theophylline on the development of dental caries in rats. six wistar dams (spf), mutans streptococci free, were obtained, each with six male pups. the dams were infected by streptococcus sobrinus 6715 and divided into three groups which received during the lactating period: (1) diet 2000; (2) diet 2000 plus caffeine (2 mg/100 g) and (3) diet 2000 plus theophylline (0.57 mg/100 g). after weaning, the pups were infected by s. ... | 2000 | 10726890 |
| glycine prevents the phenotypic expression of streptococcal glucan-binding lectin. | glycine has been used extensively in bacterial cell surface research. some researchers employ glycine in growth media so as to increase the transformability of streptococci during electroporation. others have found that glycine, similar to wall antibiotics, 'weakens' peptidoglycan. it is now shown that when glycine is incorporated into the growth medium, streptococcus sobrinus exhibits a diminished ability to aggregate with high molecular weight alpha-1,6-glucan. growth of the bacteria in either ... | 2000 | 10742601 |
| coimmunization with complementary glucosyltransferase peptides results in enhanced immunogenicity and protection against dental caries. | peptide constructs from the catalytic (cat) and glucan-binding (glu) regions of the mutans streptococcal glucosyltransferase enzymes (gtf) can provide immunity to dental caries infection. a strategy of coimmunization was tested to determine whether protection could be enhanced. rats were immunized with one of the previously described peptide constructs from the cat or glu region of the gtf of mutans streptococci or coimmunized with a combination of these constructs (cat-glu). coimmunized animals ... | 2000 | 10768962 |
| the effect of chronic clonidine administration on salivary glands and caries in the rat. | clonidine is a widely prescribed, centrally acting antihypertensive with proposed pharmacologic mechanisms of stimulation of central postsynaptic alpha(2)-adrenergic receptors and agonist activity at presynaptic alpha(2)-adrenergic receptors, interfering with peripheral regulation of norepinephrine and acetylcholine release. both of these mechanisms are capable of adversely influencing salivary output and composition, potentially leaving an individual with increased caries risk. the purpose of t ... | 2000 | 10773639 |
| current understanding of the cause of dental caries. | dental caries-associated oral streptococci are called the mutans streptococci, with streptococcus mutans and streptococcus sobrinus being the most prevalent caries-associated organisms in humans. strains of the mutans streptococci are highly conserved within not only mothers and their children but also racial groups, suggesting vertical transmission of this organism within human populations. it has been found that the mother-child infection route of the mutans streptococci can be prevented by si ... | 2000 | 10777849 |
| modulation of glucan-binding protein activity in streptococci by fluoride. | glucan-binding lectin (gbl) activity of streptococcus sobrinus was significantly reduced by fluoride in the growth medium. approximately 1.5 mm fluoride was required for a 50% reduction in gbl activity. in addition to the gbl, several other glucan-binding proteins were reduced when the bacteria were grown in subinhibitory fluoride. fluoride had no effect on glucosyltransferases (gtfs), enzymes capable of converting sucrose into alpha-1,6-glucans. all the proteins were detected by use of enhanced ... | 2000 | 10779686 |
| caries-associated micro-organisms in infants from different socio-economic backgrounds in scotland. | the aims of this study were: (1) to compare the frequency of isolation of mutans streptococci, (streptococcus mutans, steptococcus sobrinus), lactobacilli and yeasts (caries-associated micro-organisms) in the saliva of 1-year-old infants with and without dental caries; and (2) to determine if socio-economic background influenced the frequency isolation of bacteria and caries status. | 2000 | 10785295 |
| effects of resin composite components on glucosyltransferase of cariogenic bacterium. | the aim of our study was to investigate the effect of resin composite components on glucan synthesis by glucosyltransferase (gtase) derived from a cariogenic bacterium, streptococcus sobrinus b13. the eluates from cured composites stored in 0.05 mol/l potassium phosphate buffer (kpb) (ph 6.8) for 2 weeks at 37 degrees c stimulated the formation of water-insoluble glucan, whereas those from amalgam inhibited it. this finding suggests that the eluates from the resin composites enhance gtase activi ... | 2000 | 10813753 |
| in vitro antibacterial effect of rc-prep components on streptococcus sobrinus. | the aim of this study was to examine the bacteriostatic and bactericidal effects of the components of rc-prep: edta, urea peroxide and glycol. the minimal inhibitory concentration and the minimal bactericidic concentration of edta, urea peroxide and glycol were tested on the viability of streptococcus sobrinus (s. sobrinus). the combined antibacterial effect of these components was also examined on s. sobrinus. the minimal inhibitory concentration of edta was found to be 0.125%, of urea peroxide ... | 1999 | 10815566 |
| fluoride release and antibacterial properties of new-generation tooth-colored restoratives. | the aim of this study was to compare the amounts and pattern of fluoride release and antibacterial properties of new-generation restoratives over a 35-day period. materials evaluated included fluoride-releasing composites (tetric, experimental x), compomers (dyract, compoglass), and a resin-modified glass-ionomer cement (fuji ii lc). a conventional glass ionomer (fuji ii cap) was used as a control for fluoride-release testing. five samples of each restorative material were evaluated for daily fl ... | 1999 | 10823077 |
| effect of surface roughness of porcelain on adhesion of bacteria and their synthesizing glucans. | in some instances of porcelain restoration, refinishing is inevitable. in terms of plaque accumulation on porcelain, refinishing could be a substitute method for glazing. | 2000 | 10842136 |
| immunolabeling of the major cell surface protein antigen of streptococcus sobrinus with monoclonal antibody. | the purpose of this study was to determine the accessibility of monoclonal antibody (mcab), specific for the major cell surface protein antigen (pag) of streptococcus sobrinus, to the surface of its native epitopes. | 1999 | 10863403 |
| the anticariogenic effect of amine fluorides on streptococcus sobrinus and glucosyltransferase in biofilms. | dental caries is a chronic infectious disease caused by the accumulation of bacterial plaque (biofilm) on tooth surfaces. antibacterial agents, in addition to other preventive measures, can control dental plaque accumulation. amine fluorides (amf) are known anticaries agents for over 30 years. the purpose of our study was to assess the adsorption and desorption of amf to experimental dental biofilm and to evaluate the effect of amf on streptococcus sobrinus 6715 and glucosyltransferase (gtf) act ... | 2000 | 10867426 |
| studies concerning the glucosyltransferase of streptococcus sanguis. | we have shown in previous studies that the glucosyltransferase (gtf) enzymes of streptococcus mutans have distinct properties when adsorbed to a surface. in the present study, we compared the activity of gtf from streptococcus sanguis, designated gtfss, in solution and on the surface of saliva-coated hydroxyapatite (sha) beads, and determined the ability of its product glucan to support the adherence of oral microorganisms. gtf from s. sanguis 804 nctc 10904 was purified from culture supernatant ... | 2000 | 10867431 |
| caries inhibitory activity of cacao bean husk extract in in-vitro and animal experiments. | cacao bean husk extract (cbh) was examined for inhibitory effects on the caries-inducing properties of mutans streptococci in vitro and on caries development in specific pathogen-free sprague-dawley rats infected with mutans streptococci. cbh reduced the growth rate of almost all oral streptococci examined, which resulted in the reduction of acid production. furthermore, insoluble glucan synthesis by the glucosyltransferases from streptococcus mutans mt8148r and streptococcus sobrinus 6715 was s ... | 2000 | 10869475 |
| cariostatic activity of cacao mass extract. | chocolate is suspected to contain some caries-inhibitory substances. the cariostatic activity of cacao mass extract (cm), the main component of chocolate, was examined in vitro and in experimental animals. cm showed no detectable effects on the cellular growth and acid production of mutans streptococci. on the other hand, the cell-surface hydrophobicity of mutans streptococci was significantly reduced by the presence of cm. furthermore, insoluble glucan synthesis by the glucosyltransferases from ... | 2000 | 10869494 |
| nigerooligosaccharide acceptor reaction of streptococcus sobrinus glucosyltransferase gtf-i. | nigerose and nigerooligosaccharides served as acceptors for a glucosyltransferase gtf-i from cariogenic streptococcus sobrinus to give a series of homologous acceptor products. the soluble oligosaccharides (dp 5-9) strongly activated the acceptor reaction, resulting in the accumulation of water-insoluble (1-->3)-alpha-d-glucan. the enzyme transferred the labeled glucosyl residue from d-[u-13c]sucrose to the 3-hydroxyl group at the non-reducing end of the (1-->3)-alpha-d-oligosaccharides, as uneq ... | 2000 | 10877092 |
| [effect of subinhibitory concentrations of various antimicrobials on insoluble glucan production, polystyrene adherence and bacterial surface hydrophobicity of streptococcus sobrinus]. | the effect of subinhibitory concentrations (1/2, 1/4, 1/8 of the mic) of amoxicillin, teicoplanin, clindamycin and erythromycin on the factors influencing the adherence and pathogenicity of streptococcus sobrinus was evaluated. these factors included insoluble glucan, polystyrene adherence and bacterial surface hydrophobicity. insoluble glucan was detected using a spectrophotometric method; polystyrene adherence was assessed using microtiter plates; and surface hydrophobicity was determined usin ... | 1999 | 10878518 |
| shifts in membrane fatty acid profiles associated with acid adaptation of streptococcus mutans. | cells of streptococcus mutans ua159 physiologically adapted to acidification during growth at ph 5 in glucose-limited chemostat cultures were enriched in mono-unsaturated and longer chain fatty acids compared with unadapted cells grown under the same conditions but at ph 7. ratios of unsaturated to saturated fatty acids in the cells were, respectively, 1.2 and 0.3. cyclopropane fatty acids were not detected. streptococcus sobrinus 6715, which is known to have minimal acid-adaptive capacity, show ... | 2000 | 10913871 |
| inhibitory effects of plant polyphenoloxidase on colonization factors of streptococcus sobrinus 6715. | exogenously added polyphenoloxidase (ec 1.14.18.1), an enzyme which oxidizes tyrosine residues and is commonly found in many dietary components, abolished the aggregation of streptococcus sobrinus 6715 by high-molecular-weight dextran. the enzyme decreased glucan-binding lectin and/or glucosyltransferase i activities. | 2000 | 10952624 |
| differences in acidogenicity of s. sobrinus and s. rattus are linked to the catalytic efficiency of the glycolytic key enzyme phosphofructokinase. | this contribution describes the biochemical properties of two catalytically different phosphofructokinases (pfks) purified from streptococcus rattus lb 2 (pfk-rat) and streptococcus sobrinus omz 65 (pfk-sob), respectively. steady-state kinetics revealed k(m) = 0. 8 mm for pfk-rat and k(m) = 0.08 mm for pfk-sob for f-6-p as the substrate. the enzymes also differ in their ph profiles: whereas the highest activity of pfk-rat was measured at ph = 8.0, the optimum ph of pfk-sob was at ph = 7.0. in ad ... | 2000 | 11014908 |
| automated immunofluorescence for enumeration of selected taxa in supragingival dental plaque. | the present study investigated a recently developed automated image analysis technique for its applicability to the enumeration of selected bacteria in supragingival dental plaque. following initial calibration, the system is capable to count fluorescence-labeled target cells in up to 48 samples without user interference. test samples contained a characteristic mixture of planktonic bacteria, small almost planar bacterial aggregates, and large, virtually indisruptable clumps with cells from mult ... | 2000 | 11037755 |
| low-cariogenicity of trehalose as a substrate. | the effects of trehalose on cariogenesis by mutans streptococci were investigated. | 2000 | 11082525 |
| inhibitory effects of apple polyphenols and related compounds on cariogenic factors of mutans streptococci. | the inhibitory effects of apple polyphenols (app) on the synthesis of water-insoluble glucans by glucosyltransferases (gtf) of streptococci of the mutans group and on the sucrose-dependent adherence of the bacterial cells were examined in vitro. app markedly inhibited the activity of gtf purified from the cariogenic bacterial cells. however, app showed no significant effect on the growth of the cariogenic bacteria. the strongest gtf inhibitors in app were apple condensed tannins (act), a mixture ... | 2000 | 11087536 |
| site-specific oral colonization of mutans streptococci detected by arbitrarily primed pcr fingerprinting. | the clonal diversity and colonization pattern of mutans streptococci within an individual were studied by using a total of 50 tooth site samples obtained from seven 3- to 7-year-old children. five children contributed saliva samples. from the 7 subjects, 90, 74, 81, 53, 83, 103, and 114 isolates (a total of 598) representing streptococcus mutans and streptococcus sobrinus were included for arbitrarily primed polymerase chain reaction (ap-pcr) fingerprinting. the children harbored one to four dif ... | 2000 | 11093021 |
| similarity of bacteriocin activity profiles of mutans streptococci within the family when the children acquire the strains after the age of 5. | it has been shown that there is a window of infectivity for mutans streptococci between the ages of 19 and 31 months, when many children acquire mutans streptococci transmitted from their mothers. part of the children that escape this window acquire mutans streptococci at a later age. in this group, maternal transmission is expected to be less prevalent. the present study compared the bacteriocin activity profiles of mutans streptococci isolated from mothers, fathers and children when the childr ... | 2000 | 11093022 |
| strain-related acid production by oral streptococci. | acid production, in particular at low ph, is thought to be an important ecological determinant in dental caries. the aim of the present study was to determine the acid producing capability at different ph levels of 47 streptococcal strains, representing 9 species, isolated from human dental plaque. the bacteria were grown until mid log-phase under anaerobic conditions and acid production was measured in a ph-stat system at ph 7.0, 6.0, 5.5 and 5.0. at all ph values, the mean velocity of acid pro ... | 2000 | 11093023 |
| antimicrobial effect of a novel ozone- generating device on micro-organisms associated with primary root carious lesions in vitro. | the aims of this present study were (1) to assess the antimicrobial effect of ozone from a novel ozone-generating device (heolozone, usa) [0.052% (v/v) in air delivered at a rate of 13.33 ml.s(-1)] on primary root carious lesions (prcls) and (2) to evaluate the efficacy of ozone specifically on streptococcus mutans and streptococcus sobrinus. in study 1, 40 soft prcls from freshly extracted teeth were randomly divided into two groups to test the antimicrobial effect on prcls from exposure to ozo ... | 2000 | 11093025 |
| polycarboxylates inhibit the glucan-binding lectin of streptococcus sobrinus. | polycarboxylates, such as carboxymethylcellulose and hyaluronan, were found to be reversible inhibitors of the glucan-binding lectin of streptococcus sobrinus. when the carboxylate groups were coupled to ethylenediamine, or reduced with carbodiimide-borohydride, inhibitory powers were lost. similarly, n-deacetylated hyaluronan had poor inhibitory powers, probably due to the introduction of positive charges into the polymer. other polymers, such as chondroitin sulfates, dextran sulfate, fetuin, h ... | 2000 | 11099864 |
| pcr for detection and identification of streptococcus sobrinus. | oligonucleotide primers were designed based upon a comparison of the dextranase gene (dex) sequences from streptococcus sobrinus and s. mutans. the primers amplified a 1610-bp long dna fragment on the dex gene by a pcr. the pair of primers was specific to s. sobrinus as the other members of the mutans streptococci - s. mutans, s. downei, s. cricetus, s. rattus, s. macacae and s. ferus - gave no pcr products. other gram-positive oral bacteria (15 strains of 10 species of cocci and 18 strains of 1 ... | 2000 | 11129717 |
| the galu gene of streptococcus pneumoniae that codes for a udp-glucose pyrophosphorylase is highly polymorphic and suitable for molecular typing and phylogenetic studies. | the enzyme utp-glucose-1-phosphate uridylyltransferase (udp-glucose pyrophosphorylase, udpg:pp) is synthesized by practically all organisms, although prokaryotic udpg:pps are evolutionarily unrelated to the eukaryotic counterparts. the primary structure of prokaryotic udpg:pps is well conserved, although little information exists on the polymorphism of the genes coding for these enzymes. it has been reported that the galu gene encoding the streptococcus pneumoniae udpg:pp is absolutely required ... | 2000 | 11137293 |
| xylitol-induced elevated expression of the gbpc gene in a population of streptococcus mutans cells. | xylitol possesses a unique property distinct from the other caries-preventive sweeteners. this sugar alcohol cannot be metabolized to acids but is taken up by streptococcus mutans and accumulated as a toxic sugar-phosphate in the cells, resulting in growth inhibition. due to the accumulation, xylitol induces biological responses including the emergence of xylitol-insensitive populations. therefore, we expected another response induced by xylitol and found a new phenomenon, that cells repeatedly ... | 2000 | 11153929 |
| simple and rapid detection of streptococcus mutans and streptococcus sobrinus in human saliva by polymerase chain reaction. | streptococcus mutans and streptococcus sobrinus are major pathogens causing dental caries in humans. a simple and rapid method to detect these species in human saliva simultaneously was developed using the polymerase chain reaction (pcr). chromosomal dna was extracted by boiling bacterial cells in lysis solution containing 1% triton x-100. oligonucleotide primers specific for portions of the glucosyltransferase genes (gtfb of s. mutans and gtfi of s. sobrinus) were designed. after pcr using two ... | 2000 | 11154412 |
| induction of secretory immunity with bioadhesive poly (d,l-lactide-co-glycolide) microparticles containing streptococcus sobrinus glucosyltransferase. | the effect of mucosal delivery of streptococcus sobrinus glucosyltransferase (gtf) in bioadhesive poly (d,l-lactide-co-glycolide) (plga) microparticles on induction of salivary iga and serum igg antibody responses was measured in sprague-dawley rats. preparations of gtf/plga/gelatin microparticles, or plga/gelatin microparticles or gtf in alum, were administered four times at weekly intervals by intranasal or intragastric routes. two subcutaneous injections of gtf in plga/gelatin microparticles ... | 2000 | 11155176 |
| differentiation of streptococcus mutans and streptococcus sobrinus via genotypic and phenotypic profiles from three different populations. | routine identification of streptococcus mutans and streptococcus sobrinus is generally based upon growth on various selective media, colony morphology and biochemical characteristics. we examined various approaches of differentiating these two species through a combination of the conventional phenotypic methodology with chromosomal dna fingerprint (cdf) and arbitrarily primed polymerase chain reaction (ap-pcr) methods. initially, ten atcc type strains and 20 randomly selected clinical isolates o ... | 2001 | 11169134 |
| hla-dr4 and salivary immunoglobulin a reactions to oral streptococci. | the aim of this study was to describe and compare salivary immunoglobulin a (iga) antibody reactions to extracts of strains of three oral streptococci in human leukocyte antigen (hla)-dr4-positive and -dr4-negative subjects. whole paraffin-stimulated saliva samples were collected from 27 apparently healthy subjects. previous hla typing showed that 20 subjects were dr4 positive and 7 were dr4 negative. hla-drb1*04 subtyping was performed among the dr4-positive subjects. whole-cell antigen extract ... | 2001 | 11169139 |
| the role of fructans on dental biofilm formation by streptococcus sobrinus, streptococcus mutans, streptococcus gordonii and actinomyces viscosus. | dental plaque biofilm plays a pivotal role in the progression of dental diseases. polysaccharides are of great importance in the ecology of the dental biofilm. we studied the effect of fructans, glucans and a mixture of both fructans and glucans, synthesized in situ by immobilized fructosyltransferase or glucosyltransferase, on the adhesion of streptococcus sobrinus, streptococcus mutans, streptococcus gordonii and actinomyces viscosus to hydroxyapatite beads coated with human saliva (sha). the ... | 2001 | 11179653 |
| [effects of sodium doecyl sulfate on the artificial dental plaque in chemostat]. | sodium doecyl sulfate (sds) is widely used as a detergent in dentifrices. it has been shown to interfere with the protein adsorption to hydroxyapatite (ha), and inhibit acquired pellicle formation. the aim of the present study was to examine the effects of sds on the artificial dental plaque in chemostat. the amount of the 3h-labelled bacteria adhered on the enamel fragment surface was determined with scintillometer. the artificial dental plaque was observed under the scanning electron microscop ... | 1997 | 11189307 |
| adsorption of oral bacteria to porous type calcium carbonate. | the purpose of this study was to investigate the adsorption of [3h]-thymidine labeled oral microorganisms to porous type calcium carbonate (pcc) beads in a buffer containing human parotid saliva and to pcc combined chewing gum sheets. adsorption rates of streptococcus sobrinus b13 and 6715, streptococcus mutans mt8148r and actinomyces naeslundii t14v with pcc were significantly higher than those with calcium carbonate (cc) beads (p < 0.01). adsorption rates of s. sobrinus, s. mutans and a. naesl ... | 2000 | 11212584 |
| dental caries is a preventable infectious disease. | dental caries is the most common infectious disease affecting humans. the principal causative agents are a group of streptococcal species collectively referred to as the mutans streptococci of which streptococcus mutans and streptococcus sobrinus are the most important agents of human caries. this review outlines what is currently known about these ubiquitous pathogens and discusses novel methods for elimination of these bacteria from dental plaque. | 2000 | 11225524 |
| spatial arrangements and associative behavior of species in an in vitro oral biofilm model. | the spatial arrangements and associative behavior of actinomyces naeslundii, veillonella dispar, fusobacterium nucleatum, streptococcus sobrinus, and streptococcus oralis strains in an in vitro model of supragingival plaque were determined. using species-specific fluorescence-labeled antibodies in conjunction with confocal laser scanning microscopy, the volumes and distribution of the five strains were assessed during biofilm formation. the volume-derived cell numbers of each strain correlated w ... | 2001 | 11229930 |
| inhibition of bacterial and glucan adherence to various light-cured fluoride-releasing restorative materials. | this study investigated the potential plaque adhesion properties of various light-cured fluoride-releasing restorative materials by measuring the amount of adhering radiolabeled bacteria and glucan. | 2001 | 11239586 |
| cloning and dna sequencing of the surface protein antigen i/ii (paa) of streptococcus cricetus. | we have cloned and sequenced the gene encoding the surface protein antigen paa (antigen i/ii family) from streptococcus cricetus e49 (serotype a) using degenerate pcr. the deduced amino acid sequence of paa reveals two repeating regions (a region; alanine-rich region, p region; proline-rich region). two additional tandem repeats were found in the a region and part of the p region was deleted compared to antigen i/ii. homology and phylogenetic analyses reveal that paa is homologous to streptococc ... | 2001 | 11267788 |
| validation of an in vitro biofilm model of supragingival plaque. | the study of biofilm structure and function mandates the use of model systems for which a host of environmental variables can be rigorously controlled. we describe a model of supragingival plaque containing actinomyces naeslundii, veillonella dispar, fusobacterium nucleatum, streptococcus sobrinus, and streptococcus oralis wherein cells are cultivated anaerobically in a saliva-based medium on hydroxyapatite discs coated with a salivary pellicle, with material and pieces of apparatus common to al ... | 2001 | 11269730 |
| comparison of erythritol and xylitol saliva stimulants in the control of dental plaque and mutans streptococci. | the effect of 2-month usage of saliva-stimulating pastils containing either erythritol or xylitol was studied in a cohort of 30 subjects assigned to the respective polyol groups (n = 15). the daily consumption level of both polyols was 5.2 g, used in 5 daily chewing episodes. the mean weight of total plaque mass (collectable during a standard period of 3 min from all available tooth surfaces) was reduced significantly in the xylitol-group, while no such effect was observed in the erythritol-grou ... | 2001 | 11275673 |
| antibacterial properties of dentin bonding systems, polyacid-modified composite resins and composite resins. | this study examined the antibacterial activities of the bonding systems syntac, ebs and scotchbond 1, the polyacid-modified composite resins hytac and compoglass, and the composite resins tetric, z100 and scalp-it. they were evaluated using the cariogenic bacteria streptococcus mutans, lactobacillus salivarius, streptococcus sorbinus and actinomyces viscosus in vitro with a modified cylinder drop plate agar diffusion assay. all adhesives of the dentin bonding systems and the polyacid-modified co ... | 2001 | 11298264 |
| effects of three different infant dentifrices on biofilms and oral microorganisms. | the purpose of this work was to evaluate the effects of infant dentifrices: a--with lactoperoxidase, glucose oxidase and lactoferrin; b--with 1100 ppm of naf and sodium lauryl sulfate; c--with extract of calendula. the dentifrices were test on biofilms formed in vitro from saliva and dental plaque of infants, using reference strains a. viscosus (atcc 43146); c. albicans (atcc 51501); l. casei (atcc 4646); s. mitis (atcc 49456); s. mutans (atcc 25175); s. oralis (atcc 35037); s. sanguis (atcc 105 ... | 2000 | 11314149 |
| acidogenicity and acidurance of fluoride-resistant streptococcus sobrinus in vitro. | to induce fluoride-resistant mutants of streptococcus sobrinus in vitro and to study the acidogenic and acid tolerance of fluoride-resistant strains. | 2000 | 11314523 |
| antimicrobial activity of silver nitrate against periodontal pathogens. | metal ions were evaluated as potential antimicrobial agents suitable for local delivery in the oral cavity for the treatment of periodontitis. silver nitrate, copper chloride, and zinc chloride were tested for antimicrobial activity in in vitro killing assays conducted in phosphate buffered saline with a series of oral bacteria including gram-negative periodontal pathogens and gram-positive streptococci. copper and zinc salts failed to exhibit strong and consistent activity against periodontal p ... | 2001 | 11327077 |
| membranotropic effects of the antibacterial agent triclosan. | triclosan is a broad-spectrum hydrophobic antibacterial agent used in dermatological preparations and oral hygiene products. to gain further insight into the mode of action of triclosan we examined its effects on membranes by performing leakage titrations of different oral bacteria and studying its interaction with model membranes through the use of different biophysical techniques. there was negligible efflux of intracellular material from streptococcus sobrinus at the minimal inhibitory concen ... | 2001 | 11368524 |
| high-sucrose diet reduces defensive reactions of the pulpo-dentinal complex to dentinal caries in young rats. | the significance of systemic dietary effects on the response of the pulpo-dentinal complex to dentinal caries was examined. weanling rats were divided into high sucrose or control diet groups both with and without cariogenic bacterial inoculation. at the onset, tetracycline was injected to mark the dentin formation during the experiment. after 5-6 week, mandibular molars were sectioned sagittally. the areas of dentin formed during the experiment and those of dentinal caries were quantified separ ... | 2001 | 11370755 |
| analysis of bacterial detachment from substratum surfaces by the passage of air-liquid interfaces. | a theoretical analysis of the detachment of bacteria adhering to substratum surfaces upon the passage of an air-liquid interface is given, together with experimental results for bacterial detachment in the absence and presence of a conditioning film on different substratum surfaces. bacteria (streptococcus sobrinus hg1025, streptococcus oralis j22, actinomyces naeslundii t14v-j1, bacteroides fragilis 793e, and pseudomonas aeruginosa 974k) were first allowed to adhere to hydrophilic glass and hyd ... | 2001 | 11375160 |
| aspiration pneumonia: dental and oral risk factors in an older veteran population. | to investigate the importance of medical and dental factors in aspiration pneumonia in an older veteran population. | 2001 | 11380747 |
| detection of streptococcal glucan-binding proteins in biofilms. | 2001 | 11398450 | |
| growth rate and biofilm thickness of streptococcus sobrinus and streptococcus mutans on hydroxapatite. | bacteria in biofilm and planktonic bacteria exhibit different properties. the objective of the present study was to compare the growth rates of streptococcus sobrinus and streptococcus mutans on different types of biofilm with their planktonic growth rate. our experimental model consisted of hydroxyapatite beads coated with human saliva (sha). glucans or fructans were synthesized in situ on sha by immobilized cell-free glucosyltransferase or fructosyltransferase isolated from oral bacteria. s. s ... | 2001 | 11398997 |
| diepitopic construct of functionally and epitopically complementary peptides enhances immunogenicity, reactivity with glucosyltransferase, and protection from dental caries. | coimmunization with peptide constructs from catalytic (cat) and glucan-binding (glu) domains of glucosyltransferase (gtf) of mutans streptococci has resulted in enhanced levels of antibody to the cat construct and to gtf. we designed and synthesized a diepitopic construct (cat-glu) containing two copies of both cat (b epitope only) and glu (b and t epitope) peptides. the immunogenicity of this diepitopic construct was compared with that of individual cat and glu constructs by immunizing groups o ... | 2001 | 11401956 |
| dextran acceptor reaction of streptococcus sobrinus glucosyltransferase gtf-i as revealed by using uniformly 13c-labeled sucrose. | a sucrose glucosyltransferase gtf-i from cariogenic streptococcus sobrinus transferred the uniformly 13c-labeled glucosyl residue ([u-(13)c]glc) from [u-(13)c]sucrose to exogenous dextran t500 at the non-reducing-end, mostly by alpha-(1-->6) linkages and partially by alpha-(1-->3) linkages, as revealed by the 13c-(13)c nmr coupling pattern. with increasing amounts of [u-(13)c]sucrose, transfer of [u-(13)c]glc to the alpha-(1-->3)-linked chain became predominant without increase in the number of ... | 2001 | 11423107 |
| peroxidase reaction as a parameter for discrimination of streptococcus mutans and streptococcus sobrinus. | 425 strains of mutans streptococci and 12 reference strains were investigated by membrane fatty acid spectra (mfas) and peroxidase reaction (pr) after aerobic and anaerobic incubation. 423 strains were identified as streptococcus mutans. the remaining 2 strains were identified as streptococcus sobrinus. the pr of 29 strains was doubtful; immediately after anaerobic incubation a negative pr changed into a slightly positive pr. to test the diagnostic value of pr the strains were additionally inves ... | 2001 | 11423721 |
| sealing ability of furcation perforation repair. | 1998 | 11431824 | |
| inhibitory effects of moabs against a surface protein antigen in real-time adherence in vitro and recolonization in vivo of streptococcus mutans. | a surface protein antigen (pac) of streptococcus mutans, particularly the a-region of the molecule, has been reported to interact with salivary components on the tooth surface. it might be a candidate antigen inducing the production of antibodies against the adherence of s. mutans to the tooth surface. we investigated the effects of monoclonal antibodies (moabs) obtained by immunization of synthetic pac peptides that completely correspond to the amino acid sequence of part of the a-region. these ... | 2001 | 11439156 |
| a novel approach for purification of recombinant proteins using the dextran-binding domain. | using the dextran-binding domain (dbd) of a type of glucosyltransferase (gtf) from streptococcus sobrinus, we have developed a novel method for purifying recombinant proteins. dbd-tagged green and red fluorescent proteins as well as the parent gtf and dbd moiety were adsorbed well to commercially available cross-linked dextran (such as sephadex beads and sephacryl beads), and eluted efficiently with water-soluble dextran. the purity of the eluted proteins after this one-step affinity purificatio ... | 2001 | 11445073 |
| purification and characterization of an oligo-isomaltosaccharide synthase from a streptococcus sobrinus glucosyltransferase-i deficient mutant. | one glucosyltransferase (gtf) -i deficient mutant of streptococcus sobrinus strain b13n was isolated through chemical mutagenesis with ethyl methanesulfonate, and characterized. this mutant, designated as b13n-id, readily allowed us to purify a homogeneous oligo-isomaltosaccharide synthase (gtf-s) from its culture fluid. the purified gtf-s was only recognized with rabbit polyclonal antibody against recombinant gtf-s from an ecsherichia coli md124 clone expressing the b13n gtfs gene, and showed t ... | 2001 | 11471726 |
| does social deprivation in 1, 2, 3 and 4-year-old scottish infants influence the frequency isolation of caries-associated micro-organisms? | the aim of this study was to determine if there are associations between the level of social deprivation/affluence and the frequency isolation of caries-associated micro-organisms (streptococcus mutans, streptococcus sobrinus, lactobacilli and yeasts) in a large cohort of infants examined annually from 1 to 4 years of age. | 2001 | 11472804 |
| bactericidal and cytotoxic effects of sodium hypochlorite and sodium dichloroisocyanurate solutions in vitro. | the antimicrobial and cytotoxic effects of sodium hypochlorite (naocl) and sodium dichloroisocyanurate (nadcc) were evaluated and compared in vitro. the minimal inhibitory concentration and minimal bactericidal concentration of naocl and nadcc were tested for streptococcus sobrinus, streptococcus salivarius, enterococcus faecalis, and streptococcus mutans. the cytotoxic effect was assessed by using human fibroblast tissue culture. survival rate was assessed by a protein determination method. res ... | 2001 | 11485267 |
| adherence of plaque components to different restorative materials. | this study compared the amount of artificial plaque synthesized in vitro by streptococcus sobrinus on various dental materials using radioisotopes. in particular, plaque-retaining capacities of new types of ceramics were the focus of this study. specimens were fabricated from the following materials (one amalgam alloy [spherical-d], one casting gold alloy [casting gold type i], one resin composite [herculite xr] and three ceramics [vita celay blanks, ips empress and dicor mgc]). the amount of ba ... | 2001 | 11504440 |
| alteration of the coadherence of candida albicans with oral bacteria by dietary sugars. | interactions between bacterial oral flora and candida albicans are important in denture plaque formation. this study therefore first aimed to quantify the coadherence of c. albicans and bacteria by the use of a bioluminescent adenosine triphosphate (atp) assay based on the firefly luciferase-luciferin system. the second aim was to examine the effect of i) dietary sugars (used for preculture) and ii) enzymatic digestion of fungi on the coadherence. when yeast was preincubated in yeast nitrogen ba ... | 2001 | 11555304 |
| induction and 3d reconstruction of caries-like lesions in an experimental dental plaque biofilm model. | the aim of this study was to examine an in vitro dental plaque model for volumetric assessment and 3d reconstruction of experimentally bacterial induced caries-like lesions. twenty-three extracted, impacted human molars were coated with a varnish leaving a 3 x 3 mm window on the smooth surface. the teeth were coated with human saliva and streptococcus sobrinus 6715 adhered onto the enamel via a sucrose dependent mechanism. the coated teeth were incubated for 1, 7, 14 and 28 days. at the end of e ... | 2001 | 11556956 |
| in vitro antimicrobial activities of bakuchiol against oral microorganisms. | bakuchiol was isolated from the seeds of psoralea corylifolia, a tree native to china with various uses in traditional medicine, followed by extraction with ether and column chromatography combined with silica gel and octyldecyl silane. in this study, the antimicrobial activities of bakuchiol against some oral microorganisms were evaluated in vitro. the cell growth of streptococcus mutans was inhibited in a bakuchiol concentration-dependent manner, and growth of s. mutans was completely prevente ... | 2001 | 11600349 |
| the effects of benzoate and fluoride on dental caries in intact and desalivated rats. | the decline in prevalence of dental caries in some segments of the population has been attributed mainly to extensive exposure to fluoride. over the past decades, the use of fluoridated products has increased. during the same period, the consumption of food preservatives such as benzoates and sorbates has also increased substantially. benzoates, in vitro, possess antibacterial properties similar to those of fluoride and in combination with fluoride could affect caries development. in the present ... | 2001 | 11641568 |
| cariogenic potential of lactosylfructoside as determined by acidogenicity of oral streptococci in vitro and human dental plaque in situ. | the cariogenic potential of lactosylfructoside [o-beta-d-galactopyranosyl-(1-->4)-o-alpha-d-glucopyranosyl-(1<-->2)-beta-d-fructofuranoside] was estimated by experiments on oral streptococci in vitro and human dental plaque in situ. lactosylfructoside was unable to support growth of the strains of streptococcus mutans and s. sobrinus used in this study. however, it was able to support growth of strains of s. sanguis, s. mitis and s. oralis. acid was produced rapidly by cell suspensions of s. ora ... | 2001 | 11641569 |
| antibacterial effect of parabens against planktonic and biofilm streptococcus sobrinus. | tooth decay is an infectious disease caused by bacteria immobilized on the tooth surfaces. eradication of these bacteria, for example streptococcus sobrinus (s. sobrinus), from the oral cavity is essential in the prevention and treatment of tooth decay. we have tested the antimicrobial effect of several paraben derivatives such as methyl (mp), ethyl (ep), propyl (pp) and butyl (bp) against immobilized and planktonic s. sobrinus. the antibacterial effect was as follows: mp>ep>pp=bp on immobilized ... | 2001 | 11738348 |