Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| influence of temperature on ionic sparing effect and cell-associated cations in the moderate halophile, micrococcus varians var. halophilus. | cells of the moderately halophilic micrococcus varians var. halophilus grew well in a chemically defined medium containing 1 to 3 m nacl and 0.0103 m k+. the requirement for nacl could be partially replaced by k+,:li+ and cs+. the efficiency of the sparing effect of these cations for nacl was in order of k+ greater than li+ greater than cs+. increase in growth temperature was found to enchance the sparing effect of li+ and cs+ but not that of k+. over the range of nacl concentrations in which th ... | 1979 | 542134 |
| identification of clumping-factor-negative staphylococci isolated from cows' udders. | identification to species level was attempted on a collection of 954 cultures of catalase-positive, clumping-factor- and beta-haemolysin-negative gram-positive cocci isolated from teats and milk of cows. eighty-seven per cent of the strains were identified as staphylococcus xylosus, s epidermidis, s sciuri, s haemolyticus, s hyicus subsp hyicus and chromogenes, s simulans and s cohnii. nine per cent of the collection belonged to another group which could not be identified with any of the known s ... | 1979 | 542718 |
| association between azotobacter and other soil bacteria and its effect on nitrogen fixation. | the association between a. vinelandii and either agrobacterium sp. or micrococcus sp., which are usually found as contaminants in azotobacter cultures, was investigated. in comparison with pure cultures, association increased the microbiol counts in addition to increasing nitrogen fixation rates and efficiency. in liquid cultures higher azotobacter densities were observed in the top 5 cm of the column concomitant with lowering the economic coefficient of utilization of carbonaceous compounds, wh ... | 1979 | 543344 |
| science of hemolytic activity of some radiation-resistant micrococci in food. | micrococci resistant to 1 mrad of gamma radiation were isolated from irradiated chicken. three isolates were hemolytic on blood agar plates and were selected for further study. two other radiation-resistant micrococci, micrococcus radiodurans and micrococcus radiophilus, were included in the study because there is only a very limited amount of information regarding hemolytic activity of these organisms and their potential role of public health importance. tests to determine hemolytic patterns, h ... | 1979 | 543703 |
| [microfloral study of bull seminal fluid stored at low temperatures]. | hundred twenty three samples of bull semen fluid frozen at 196 degrees c including 83 plastic ampules, 20 granules and 20 plastic straws obtained from the containers of the insemination stations of 10 farms from the sofia district were investigated. two hundred twelve strains were isolated and identified as: escherichia coli--25 strains, hafnia--16 strains, citrobacter, enterobacter and proteus mirabilis--9 strains of each. the remaining gram-negative genera and species were more rarely encounte ... | 1979 | 545852 |
| [endemic eye diseases in samoa, 1910-1913]. | in the years 1910/13 two endemic infections of the conjunctiva were found in samoa in addition to ocular manifestations of filiariosis. one of these infections was obviously trachoma, the other an acute micrococcus catarrhalis conjunctivitis by diplococcus samoensis. | 1979 | 552013 |
| hydrophobic chromatography of prenyl transferases. | several prenyl transferases were examined with respect to their affinity for c0--c10 alkyl-agarose columns. c10-alkyl agarose was effective in adsorbing each of the prenyl transferases tested but only undecaprenyl pyrophosphate and octaprenyl pyrophosphate synthetases could be effectively eluted with triton x-100. | 1979 | 556316 |
| bacteriolytic activity of normal and pathological cerebrospinal fluid. | the bacteriolytic activity of 153 samples of cerebrospinal fluid (csf) from patients with various diseases was measured by determining the ability of the csf to cause lysis of a suspension of killed cells of micrococcus lysodeikticus. normal csf did not show significant bacteriolytic activity. a high activity was found in patients with bacterial meningitis (mainly meningococcal), only to some extent correlated with the protein and cell content of the csf. slight elevation of protein and cell con ... | 1977 | 557278 |
| dna repair in v-79 cells treated with combinations of ultraviolet radiation and n-acetoxy-2-acetylaminofluorene. | earlier experiments on human cells showed that n-acetoxy-2-acetylaminofluorene mimics ultraviolet radiation in biological and repair characteristics and that the amount of repair from a combined treatment was additive. chinese hamster v-79 cells are less proficient than human cells in excision repair of pyrimidine dimers resulting from irradiation. we therefore investigated the combined effects of both agents on repair in v-79 cells to see whether they follow the same pattern as in human cells. ... | 1977 | 560253 |
| role of lysozyme in the microbicidal activity of rat alveolar macrophages. | lysozyme release from alveolar macrophages is stimulated by exposure to particles, such as latex and zymosan, and to bacteria. rat alveolar marcophages contain 10-fold-greater intracellualr concentrations of lysozyme and release more lysozyme after stimulation than rat blood neutrophils. during 30 min of incubation in vitro, alveolar macrophages kill more than 99% of micrococcus lysodeikticus in the incubation mixture, whereas neutrophils kill approximately 50% of the bacteria. the bactericidal ... | 1977 | 561033 |
| [action of egg lysozyme on representatives of the family micrococcaceae. its action on micrococcus]. | the results of the study of the effect of various concentrations of egg lysozyme on m. luteus and m. varians using 2 methods, i.e. serial dilutions in agar and turbidimetric are presented. it was found that the mic of lysozyme for m. luteus ranged within wide limits, from less than 0.0003 to 1 mg/ml. m. varians was stable to lysozyme. the mic for all the strains was 8 mg/ml. the turbidimetric method provided determination of general regularities in changes of the optical density in all the strai ... | 1978 | 567955 |
| the effect of nitrate and nitrite on the microbial flora of wiltshire bacon after maturation and vacuum-packed storage. | 1978 | 568133 | |
| [action of egg lysozyme on representatives of the family micrococcaceae. the action on staphylococci]. | the results of the study on the effect of various concentrations of lysozyme on staphylococci of three species, i.e. staphylococcus aureus, staphylococcus epidermidis and staphylococcus saprophyticus are presented. the study was performed with the use of the turbidimetric method. it was found that all the staphylococci had a common tendency for changing the optic density (od) of the suspensions under the effect of lysozyme which was different from that in micrococcus luteus. however, the dynamic ... | 1979 | 572654 |
| influence of anti-micrococcus immunoglobulins on the proliferation of normal immunocompetent cells in inbred mice. | 1979 | 575106 | |
| urinary tract infection in young women, with special reference to staphylococcus saprophyticus. | acute urinary tract infections in young women attending a clinic for sexually transmitted diseases were caused by the same bacteria, in the same proportions, as those that caused infections in women students. staphylococcus saprophyticus biotype 3 (formerly called micrococcus subgroup 3) was the commonest organism after coliform bacilli and caused about 30% of the infections. it was uncommon in women over 25 years of age and rarely caused asymptomatic bacteriuria in pregnancy. most infections, i ... | 1978 | 580432 |
| [microflora in milk powder and baby food in powder form (author's transl)]. | after a discussion of the microbiological standards for milk powder and powdered baby food in various countries, the results of the microbiological analyses of some 1500 samples of these foodstuffs were presented. it emerged that the microflora of these products consists mainly of lactobacteria (they are not demonstrable with all culture methods), micrococci and bacilli: the latter are those with the greatest importance with respect to food hygiene. in addition to these clostridia and moulds are ... | 1978 | 580516 |
| [a trial on nutritionally forced mating of conventional glutamate-producing bacteria]. | by nutritionally forced mating, we tried to find the sex factor in glutamate producing bacteria with some auxtrophic mutants of corynebacterium glutamicum, micrococcus glutamicus and brevibacterium divaricatum. we failed to find true mates in this trial, even when the cells cured by acridine orange were used to mate with non-cured ones. there were some positive growth when two densed cell suspensions were dropped on the minimum medium, but after subcultured on the minimum medium no growth was fo ... | 1979 | 583276 |
| immunoadjuvant activities of the enzymatic digests of bacterial cell walls lacking immunoadjuvancy by themselves. | 1977 | 588233 | |
| purification and characterization of the dna-dependent rna polymerase and its subunit sigma from micrococcus luteus. | dna-dependent rna polymerase from micrococcus luteus can be isolated from cell extracts after removal of an excess of nucleic acids by fractionation with ammonium sulfate, followed by two consecutive gel filtrations through agarose and chromatography on cellulose phospate. either homogeneous holoenzyme or a mixture of core and holoenzyme is obtained in this way, as is indicated by electrophoresis in polyacrylamide gels in the absence of detergent, where core enzyme migrates ahead of holoenzyme. ... | 1977 | 590942 |
| membrane-bound carotenoid in micrococcus luteus protects naphthoquinone from photodynamic action. | 1977 | 593378 | |
| photokilling of micrococcus roseus. | 1977 | 594175 | |
| the photoinactivation of the respiratory chain in sarcina lutea (micrococcus luteus) and protection by endogenous carotenoid. | 1977 | 594177 | |
| [biological characteristics of culture 6734-21 and the conditions for isolating the antiviral antibiotic it produces]. | an actinomyceteous strain no. 6734-21 was isolated from a soil sample of central asia and classified as actinomyces bottropensis. it inhibited the development of the phage of the lysogenic culture of micrococcus lysodeicticus 53-40 (no. 5) and the variolovaccine dna-containing virus. actinomyces bottropensis, strain no. 6734-21 produced an antiviral antibiotic classified as one belonging to the actinorodine group. | 1977 | 596850 |
| temporal study of the staphylococci and micrococci of normal infant skin. | staphylococcus and micrococcus populations were collected from the healthy skin of 10 infant subjects. infants were sampled from 1 day to 32 weeks of age. species were characterized by approximately 30 different morphological, physiological, and biochemical characters. staphylococci were the predominant inhabitants of normal skin, whereas micrococci were found only occasionally in this environment. staphylococcus epidermidid, s. haemolyticus, and s. hominis were the predominant and persistent st ... | 1977 | 596871 |
| microbial conversion of dl-2-amino-delta2-thiazoline-4-carboxylic acid to l-cysteine and l-cystine: screening of microorganisms and identification of products. | microorganisms able to form l-cysteine from dl-2-amino-delta2-thiazoline-4-carboxylic acid (dl-atc), a chemical intermediate in the synthesis of dl-cysteine, were isolated from soil samples and classified as pseudomonas sp., pseudomonas cohaerens, p. desmolytica, and p. ovalis. thirteen l-cysteine-producing bacteria were also found in among 463 stock cultures representing 37 genera. these were achromobacter delmarvae. alcaligenes denitrificans, bacillus brevis, brevibacterium flavum, enterobacte ... | 1977 | 596877 |
| [variation of cell membrane lipid composition by means of lipid transfer proteins. properties of the protoplast membrane of micrococcus lysodeikticus after incorporation of phosphatidylcholine]. | after incorporation of phosphatidylcholine (pc) into the protoplast membrane of m. lysodeikticus by protein mediated transfer from pc liposomes, the activity of some membrane bound respiratory chain enzymes was studied. it was found that incorporation of pc decreases the rates of oxidation of exogenous substrates (nadh, malate) but the level of endogenous respiration was not changed. ferricyanidreductase activity of ghosts of m. lysodeikticus was not dependent upon the pc content of protoplasts. ... | 1977 | 597511 |
| [the protective role of pigments against uv rays in fungi isolated from the mesosphere]. | five among six species of microorganisms isolated from the mesosphere contained pigments which made them more resistant to the action of uv as compared to pigmentless microorganisms in the atmosphere of earth. uv irradiation in the atmosphere is supposed to select resistant pigmented forms, so that they predominate in the mesosphere. to confirm this assumption, mutants of aspergillus niger, penicillium notatum and circinella muscae were sported by irradiating them four times and then subjecting ... | 1977 | 600090 |
| [pattern of growth and metabolism of thermotolerant microorganisms on media containing carbohydrates and hydrocarbons]. | experiments were carried out to examine the growth and metabolism of thermotolerant yeast candida tropicalis k-41 and bacteria micrococcus freudenreichii that do not have a single temperature point but instead have an optimal temperature plateau at which the growth rate and biosynthetic activity remain unaltered or change insignificantly. upon transition from the carbohydrate to the hydrocarbon pattern of nutrition these microorganisms show significant changes in metabolic processes: optimal con ... | 1977 | 600921 |
| [synovectomy in the treatment of acute arthritis caused by micrococcus pyogenes]. | in addition to antibiotic therapy associated with immobilization in a plaster cast, effective in the majority of cases of acute arthritis caused by st. pyogenes and resection-arthrodesis which is necessary in the stage of osteoarthritis, synovectomy occupies an intermediate position. during a period--possibly not a short one--when the lesions are still purely synovial and do not regress under medical treatment, their ablation permits recovery from the infection with restoration of the functional ... | 1977 | 609879 |
| properties of the halophilic nuclease of a moderate halophile, micrococcus varians subsp. halophilus. | the halophilic nuclease of micrococcus varians atcc 21971 hydrolyzed thymidine 5'-monophospho-p-nitrophenyl ester at a rate that increased with the nacl concentration up to saturation. the nuclease attacked rna and dna exonucleolytically and processively, producing 5'-mononucleotides. the molecular weight of the enzyme as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis was 99,000, approximately the same as that previously determined for the native enzyme. examination of a ... | 1978 | 618848 |
| upper boundary of the biosphere. | by using meterological rockets fitted with specially designed analyzers, samples for microbiological investigation have been taken. the analyzer design prevented extraneous microorganisms from penetrating into the analyzer. before being used, the analyzers were sterilized with high gamma-ray doses. for the first time microorganisms have been detected in the mesosphere at an altitude of 48 to 77 km. the microorganisms are microscopic fungi having black conidia or spores (circinella muscae, asperg ... | 1978 | 623455 |
| pulse fluorimetry study in polarized light of dna-ethidium bromide complexes. | in previous works, a quantitative analysis of the fluorescence anisotropy decay, based on a comparison of the experimental measurements with a monte carlo simulation of the excitation energy migration, has been shown to provide the value of the unwinding angle of the dna helix, induced by an ethidium bromide (e.b.) molecule intercalation. in the present work some of the characteristics of the model used in the computation are reexamined: namely the influence of the direction of the e.b. electron ... | 1978 | 623872 |
| lipid intermediates in the biosynthesis of the linkage unit between teichoic acids and peptidoglycan. | 1978 | 624402 | |
| complex nature of serum lysozyme activity: evidence of thermolability in inflammatory bowel disease. | in patients with crohn's disease and ulcerative colitis, alterations in serum storage temperature produced significant changes in serum lysozyme activity (sla) as measured by the lysoplate method. this was not the case in healthy controls or in a group with other gastrointestinal disorders. electrophoretic separation of serum revealed two components of lysozyme-type lytic activity but only one in extracts of gut mucosa, leucocytes, and egg white. the major lytic component of serum migrated towar ... | 1978 | 627614 |
| septic shock with micrococcus luteus. | micrococcus luteus is considered a non-pathogenic saprophyte of human skin and eye. disease in man caused by this organism is not recorded in medical literature. we present a case of septic shock cause by m luteus. the value of this report is to document the pathogenicity of coagulase-negative staphylococci in patients without valvular heart disease, surgically implanted artifificial prosthetic devices, or polyethylene intravenous catheters. | 1978 | 629642 |
| the linkage of sugar phosphate polymer to peptidoglycan in walls of micrococcus sp. 2102. | 1. protein-free walls of micrococcus sp. 2102 contain peptidoglycan, poly-(n-acetylglucosamine 1-phosphate) and small amounts of glycerol phosphate. 2. after destruction of the poly-(n-acetylglucosamine 1-phosphate) with periodate, the glycerol phosphate remains attached to the wall, but can be removed by controlled alkaline hydrolysis. the homogeneous product comprises a chain of three glycerol phosphates and an additional phosphate residue. 3. the poly-(n-acetylglucosamine 1-phosphate) is atta ... | 1978 | 629757 |
| a circular dichroism study of dna.platinum complexes. differentiation between monofunctional, cis-bidentate and trans-bidentate platinum fixation on a series of dnas. | the circular dichroism (cd) spectra of a series of dna . platinum complexes are presented. the following platinum compounds, [pt(dien)cl]cl, cis-pt(nh3)2cl2, cis-pt(en)cl2, trans-pt-(nh3)2cl2, k[pt(nh3)cl3] and k2[ptcl4] were complexed with the dna extracted from bacteria micrococcus lysodeikticus (72% dg + dc), escherichia coli (50% dg + dc), clostridium perfringens (32% dg + dc) and salmon sperm (41% dg + dc). strong differences were found between the different dna . pt complexes. three types ... | 1978 | 631125 |
| the origin of the electrophoretic microheterogeneity in primer-independent polynucleotide phosphorylase from micrococcus luteus. | 1978 | 632278 | |
| rapid test for the serological separation of staphylococci from micrococci. | a simple test for the serological separation of staphylococci from micrococci is described, which is based on the quite different cell wall peptidoglycan structures of these two genera. antisera to (pentaglycyl-epsilon-amino-n-hexanoic acid)20-albumin agglutinated without exception all staphylococci and gave no positive reaction with micrococci or other bacterial cells. to obtain a good reaction, it was necessary to extract the cells with hot trichloroacetic acid for 30 min. antisera to (tri-l-a ... | 1978 | 637545 |
| on the relationship between glycerophosphoglycolipids and lipoteichoic acids in gram-positive bacteria. i. the occurrence of phosphoglycolipids. | 1. gram-positive bacteria out of the families of streptococcaceae, lactobacillaceae, micrococcaceae and bacillaceae were investigated with respect to the occurrence and the concentration of phosphoglycolipids. 2. phosphatidylglycolipids occur exclusively in group d streptococci and in streptococcus hemolyticus d-58. phosphatidyl-alpha-kojibiosyldiacylglycerol, the prevalent species, accounts for up to 28% of the polar lipids. the related glycerophospho-phosphatidyl-alpha-kojibiosyldiacylglycerol ... | 1978 | 638158 |
| repair of the double-strand breaks produced by 125i disintegrations in the dna of micrococcus radiodurans. | wild-type m. radiodurans and two radiosensitive mutants were used to study the lethal effects of 125i disintegrations in their dna. the relative sensitivities of these three strains to inactivation by gamma-radiation were reflected in their relative sensitivities to inactivation by 125i decay. the number of double-strand (ds) breaks in the dna appeared to be similar at levels of gamma-radiation and of 125i decay that reduced survival to 10%. all three strains of m. radiodurans rapidly repaired d ... | 1978 | 639557 |
| interactions between the diatom thallasiosira pseudonanna and an associated pseudomonad in a mariculture system. | the marine diatom thallasiosira pseudonanna (3h) and several bacteria associated with it were isolated from batch cultures at the university of delaware mariculture facility. the interaction between the algae and each of the bacteria was investigated. one of the isolates, t827/2b (pseudomonas sp.), was incapable of surviving in f/2 culture medium unless the algae were present. when the algae and t827/2b were grown together in the f/2 medium, the bacterial growth was stimulated and the algal grow ... | 1978 | 646360 |
| [determination of the molecular weight and radius of gyration of lactobacillus 30a histidine decarboxylase and micrococcus sp. n. histidine decarboxylase]. | 1978 | 647048 | |
| the envelope of micrococcus radiodurans: isolation, purification, and preliminary analysis of the wall layers. | two methods are presented that separate the complex envelope of micrococcus radiodurans, strain sark, into its constituent layers. the first involved treating whole cells with 0.025 m tris buffer (ph 7.5) containing 2 mm of calcium and 3 mm of magnesium, resulting in the degradation of an intermediate ('compartmentalized') layer and consequent sloughing of the outer subunit and interior layers to form vesicles. this treatment also appears to show that the interior layer may be connected with the ... | 1978 | 647473 |
| [steroid saponin, deltonin, a new inhibitor of the respiratory chain in bacteria]. | 1978 | 648320 | |
| multiplicity of genome equivalents in the radiation-resistant bacterium micrococcus radiodurans. | the complexity of the genome of micrococcus radiodurans was determined to be (2.0 +/- 0.3) x 10(9) daltons by dna renaturation kinetics. the number of genome equivalents of dna per cell was calculated from the complexity and the content of dna. a lower limit of four genome equivalents per cell was approached with decreasing growth rate. thus, no haploid stage appeared to be realized in this organism. the replication time was estimated from the kinetics and amount of residual dna synthesis after ... | 1978 | 649572 |
| enzymes from micrococcus luteus involved in the initial steps of excision repair of spontaneous dna lesions: uracil-dna-glycosidase and apurinic-endonucleases. | uracil-dna-glycosidase that releases free uracil from single-stranded or double-stranded deaminated dna and poly d(a-u) has been partially purified from micrococcus luteus. the enzyme has a molecular weight of about 16,000 and can be separated from uracil-endonuclease and endonucleases (ap-endonucleases) specific for apurinic and apyrimidinic sites. uracil-dna-glycosidase does not act on guanine residues opposite uracil in double-stranded dna and on xanthine in deaminated dna. the glycosidase ge ... | 1978 | 652527 |
| [analysis of the germ content of the air in university lecture rooms (author's transl)]. | the microflora of the air in university lecture rooms was examined with the sedimentation method. for this purpose blood agar (20 degrees c and 37 degrees c), saline agar and endo plates were used. the germ counts identified correlate to the size and utilization of the rooms. the lowest germ count was determined in the room used for practical work, which was followed by the lecture room. the newly built lecture room contained the highest colony count. the floor ventilation furnishes an explanati ... | 1978 | 654689 |
| antibodies against a synthetic peptidoglycan-precursor pentapeptide cross-react with at least two distinct populations of uncross-linked soluble peptidoglycan secreted by micrococcus luteus cells. | 1978 | 658041 | |
| action of mycosubtilin and of bacillomycin l on micrococcus luteus cells and protoplasts: influence of the polarity of the antibiotics upon their action on the bacterial cytoplasmic membrane. | 1978 | 658439 | |
| repair of alkylated dna in chinese hamster cells measured by loss of enzyme-sensitive sites in isolated dna. | 1978 | 666813 | |
| the radiation-releasable cell wall nuclease of micrococcus radiodurans. purification and properties of the native enzyme. | micrococcus radiodurans is known to possess a surface nuclease located in a mid-wall layer. previous work showed that hydroxyl radicals, generated by sublethal doses of ionizing radiation, attack the cell wall and initiate release of the active enzyme into the external medium. the enzyme from unirradiated cells has now been purified to homogeneity by a simple 3-step process. the nuclease is a dimer of molecular weight about 260 000 and exonucleolytically degrades both dna and rna to 5'-mononucle ... | 1978 | 667080 |
| endocarditis associated with cardiac catheterization due to a gram-positive coccus designated micrococcus mucilaginosus incertae sedis. | a gram-positive coccus, presently named micrococcus mucilaginosus incertae sedis, was isolated from 14 blood cultures from a patient with endocarditis. the first positive blood culture was drawn 5 days after the patient underwent cardiac catheterization. | 1978 | 670378 |
| polymicrobial bacteremia associated with pharyngotonsillitis. | 1978 | 671160 | |
| demonstration of the physiological role of autolysis by a comparative study with a wild-type and its non-autolytic mutant of micrococcus lysodeikticus (luteus) cultivated with externally added proteolytic enzymes. | the log phase cells of autolytic microccus lysodeikticus (luteus) ifo 3333 did not autolyze when grown in the presence of trypsin although the growth curve and morphology of the cells were not influenced. a non-autolytic mutant was obtained by subculture of the wild-type strain ifo 3333 on an agar slant containing 1% glucose. the mutant (strain mt) was wild-type ifo 3333 which occurred singly or in irregular masses. the mutant mt grown in a culture medium containing trypsin caused remarkable alt ... | 1978 | 672671 |
| [microflora of the low-mineralized waters of the truskavets deposit]. | waters of the truskavetz deposit with a low content of mineral substances were found to contain considerable amounts of microorganisms; upto millions of cells per 1 ml. sulphate-reducing, dentrifying and iron bacteria were detected in the waters. the following genera were represented and isolated: pseudomonas, bacillus, nocardia, arthrobacter, brevibacterium, corynebacterium, micrococcus, candida, cryptococcus, rhodotorula, hansenula, torulopsis, trichosporon. many of the studied strains assimil ... | 1978 | 672688 |
| four mutants of micrococcus radiodurans defective in the ability to repair dna damaged by mitomycin-c, two of which have wild-type resistance to ultraviolet radiation. | 1978 | 672894 | |
| increased sensitivity of uv-repair-deficient human cells to dna bound platinum products which unlike thymine dimers are not recognized by an endonuclease extracted from micrococcus luteus. | we have studied the response of human cells in culture to cis platinum[ii] diammine dichloride (cis pt[ii]) induced dna damage. the survival data, measured as a function of cis pt[ii] dose were similar in a normal cell line (human foetal lung) compared to a uv-sensitive, thymine dimer excision repair-deficient cell line (xeroderma pigmentosum). however, there was a marked difference between the two cell lines when binding to dna was plotted against dose of cis pt[ii] given for 1 h. when these fi ... | 1978 | 672924 |
| airborne bacteria in an urban environment. | samples were taken at random intervals over a 2-year period from urban air and tested for viable bacteria. the number of bacteria in each sample was determined, and each organism isolated was identified by its morphological and biochemical characteristics. the number of bacteria found ranged from 0.013 to 1.88 organisms per liter of air sampled. representatives of 19 different genera were found in 21 samples. the most frequently isolated organisms and their percent of occurence were micrococcus ... | 1978 | 677875 |
| interactions of bacillus licheniformis atcc 10716 and normal flora of human skin. | to determine whether antibiotic production might be ecologically advantageous in the survival of bacillus species on human skin, we applied spores of a bacitracin-producing strain of bacillus licheniformis (atcc 10716) to the forearms of 11 volunteers. three additional strains of b. licheniformis which did not synthesize antibiotics, including a mutant of atcc 10716, were used in subsequent control trials. samples of flora were taken from inoculated and control (opposite forearm) sites during th ... | 1978 | 677879 |
| subunit structure of micrococcus luteus catalase. dissociation of m. luteus catalase induced by dodecylsulfate, citraconic and 2,3-dimethylmaleic anhydrides and urea. | m. luteus catalase dissociates upon treatment with urea, dodecylsulfate and anhydrides into monomers, the molecular weight of which appears to be 1/4 of that of the native enzyme. the urea-induced dissociation depends upon the incubation time, the urea concentration and the ph of the incubation mixture. reassociation of the subunits proved to be unsuccessful. native m. luteus catalase only contains 30% alpha-helix. when fully dissociated in presence of urea, it still retains 15% alpha-helix. cat ... | 1978 | 680645 |
| characterization of micrococcaceae isolated from post mortem examined pigs. | the investigation comprised 170 cases of infections due to micrococcaceae among post mortem examined pigs that died during the preweaning period. the incidence was 3.1 promille of the live-born pigs. the infection was most frequent among pigs less than 4 weeks of age (table i). acute and subacute generalized infections occurred in 43.5% of the cases (table ii), and exudative epidermitis was the cause of death in 23.5%. in the remaining 32.9% of the cases, the post mortem findings included a vari ... | 1978 | 683856 |
| bifunctional intercalation and sequence specificity in the binding of quinomycin and triostin antibiotics to deoxyribonucleic acid. | quinomycin c, triostin a and triostin c are peptide antibiotics of the quinoxaline family, of which echinomycin (quinomycin a) is also a member. they all remove and reverse the supercoiling of closed circular duplex dna from bacteriophage pm2 in the fashion characteristic of intercalating drugs, and the unwinding angle at i 0.01 is, in all cases, almost twice that of ethidium. thus, as with echinomycin, they can be characterized as bifunctional intercalating agents. for the triostins this conclu ... | 1978 | 687363 |
| the structure of a periodic cell wall component (hpi-layer of micrococcus radiodurans). | the hexagonally packed interlayer (hpi-layer) from micrococcus radiodurans cell walls has been studied by electron microscopy and subsequent digital image processing. the most prominent feature in the average images is a "complex" shaped like a "toothed wheel", which is perforated by a central pore and interconnected by fine spokes. this basic structural element is tentatively interpreted to represent the bulk of hpi-layer protein, intercalated by the other constituents: lipids, carotenoids and ... | 1978 | 689244 |
| serological investigations on the polylysogeny of micrococci. | specific antisera against 11 micrococcal phages could be produced by immunization of rabbits. using the neutralisation test all antisera were tested against their homologous and heterologous phages. the corresponding k-values were calculated. it could be demonstrated that phages coming from the same micrococcal donor strain can be of different genetical relationship. therefore it was concluded that micrococci can be not only lysogenic but also polylysogenic. | 1978 | 696066 |
| experiences with the typing of coagulase-negative staphylococci and micrococci. | strains of coagulase-negative staphylococci and micrococci from many sources were biotyped and tested with a set of 20 phages, 19 of which were described by dean et al. strains resistant to many antibiotics were generally untypable with these phages. nearly 50% of untypable strains could be typed by "reverse" typing--the characterisation of strains by the pattern of lysis given by their supernates on the propagating strains for the typing phages. this method was also used to clarify the relation ... | 1978 | 696068 |
| specific antibodies to the n-termini of the interpeptide bridges of peptidoglycan. | the synthetic peptides gly5-epsilon-ahx and l-ala3-epsilon-ahx, with structural similarity to the interpeptide bridge peptides of staphylococci or micrococci, respectively, were convalently linked to human serum albumin via their carboxylgroups. antisera to these synthetic peptidyl-protein antigens contained fairly high amounts of antibodies with specificity to the n-terminal parts of the peptide chains attached to the carrier proteins. antisera to (gly5-epsilon-ahx)20-albumin gave, without exce ... | 1978 | 697508 |
| an exonuclease activity associated with dna polymerase i of micrococcus radiodurans. | an exonuclease activity is associated with one of three dna polymerase in micrococcus radiodurans. the nuclease activity co-sedimented with its dna polymerase i of this bacterium on glycerol gradient centrifugation. both activities show the same optimum ph and heat-inactivation kinetics. this nuclease hydrolyzes preferentially double-stranded dna in an exonucleolytic manner from both ends of the duplex dna. the products of hydrolysis are mostly deoxyribonucleoside 5'-monophosphate and no nucleos ... | 1978 | 698224 |
| [quaternary structure of histidine decarboxylase according to small-angle x-ray diffraction and electron microscopic findings]. | the data on small angle x-ray scattering with histidine decarboxilase (hdc) from micrococcus sp. n. were analysed and a line of succesively improving approximations of the molecule shape was found: by oblate ellipsoid a:b:c = 1:10.63, by continuous cylinder and hollow cylinder with h = 50 a, 2r = 76 a, 2r = 8a. biochemical data and electron micrographs of hdc obtained made possible to distinguish subunits and thus to increase resolution of the model. the model of the enzyme molecule consisting o ... | 1978 | 698248 |
| [growth of micrococcus lysodeikticus bacteria on a deuterated medium]. | the object of this work was to prepare deuterated growth media and to adapt micrococcus lysodeikticus to a medium containing deuterated-substituted organic substances and deuterium oxide instead of water. m. lysodeikticus was grown on a medium prepared from the "deuterated-cells" of chlorella, and was capable of absorbing selectively protons from such a medium containing high concentrations of deuterium. its deuterated cells ("monsters") produced structures consisting of several (up to 8) smalle ... | 1978 | 703645 |
| [drop method of determining micrococcal and staphylococcal sensitivity to egg lysozyme]. | two methods for determination of staphylococcal and micrococcal sensitivity to lysozyme, i.e. the method of serial dilutions in agar and the drop method developed by the authors were compared. the drop method is a modification of the procedure described by kloos et al. close correlation between these two methods (r = 0.97 +/- 0.018) was found. the regression curve providing determination of the lysozyme mic with the drop method was plotted. the drop method is more simple and economical as compar ... | 1978 | 707997 |
| binding of hedamycin to deoxyribonucleic acid and chromatin of testis and liver. | the binding of the antibiotic hedamycin to dna was evaluated by density gradient centrifugation in cscl to determine the type i binding, which is essentially irreversible at high and low ionic strength. exhaustive dialysis at low ionic strength was used to determine the sum of type i and type ii binding (irreversible at low ionic strength but reversible at high ionic strength). the maximum ratio of hedamycin to dna nucleotides (rf) is 0.1 for type i and 0.1 for type ii binding to free dna, but t ... | 1978 | 708708 |
| a new assay system of phospholipid exchange activities using concanavalin a in the separation of donor and acceptor liposomes. | a new assay system of phospholipid exchange activities is described. the exchange activities were quantitated by measuring the stimulation of phospholipid transfer between two separate populations of liposomes, which contained, as the major constituents, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, sphingomyelin, and cholesterol in molar ratios of 6 :2 : 1: 1: 5. one population of the liposomes was made reactive to concanavalin a by the incorporation of 1.8 mol% alpha-d-m ... | 1978 | 708730 |
| dissimilatory nitrate reduction by liquid membrane encapsulated cell-free extracts and whole cells of micrococcus denitrificans. | the combination of sephadex g-15 and ion-exchange resin columns allows one-step desalting and separation of cellodextrins using water as the sole eluent. the column apparatus described in this paper has the potential of producing up to 3 g cellodextrins in one day. in addition, the columns described are stable and do not require repacking or regeneration after each run. hence the potential exists for scaling up this system for even greater production of cellodextrins if need be. | 1978 | 708860 |
| purification and characterization of an endonuclease from micrococcus luteus that acts on depurinated and carcinogen-modified dna. | an endonuclease which is active with regard to depurinated, alkylated, arylated, and arylamidated dna has been purified 500-fold from micrococcus luteus. in this purification, separation from the pyrimidine-dimer-specific ultraviolet-endonuclease has been achieved. the enzyme has a molecular weight of 30000 on the basis of gel filtration; its activity is not absolutely dependent upon the presence of mg2+, but 5--30 mm mg2+ produces a five-fold stimulation. potassium chloride concentrations of le ... | 1978 | 710410 |
| manipulation of phospholipid composition of membranes with the aid of lipid exchange proteins. incorporation of phosphatidylcholine into protoplasts of micrococcus lysodeikticus. | incubation of micrococcus lysodeikticus protoplasts with phosphatidylcholine liposomes and rat liver exchange proteins (ph 5.1 supernatant fraction) resulted in replacement of about one half of the bacterial total phospholipids by phosphatidylcholine. protoplasts modified by phosphatidylcholine showed a decreased rate of oxidation of exogenous substrates (nadh, malate) and decreased ferricyanide reductase activity as compared to the initial protoplasts. at the same time incorporation of phosphat ... | 1978 | 710432 |
| [comparative study of the action of different antibiotics on the membrane dehydrogenase activity in micrococcus lysodeikticus]. | the object of this work was to study the effect of antibiotics belonging to the groups of penicillin, tetracycline and aminoglycosides on the activity of lactate dehydrogenase, alcohol dehydrogenase and malate dehydrogenase in the membranes of micrococcus lysodeikticus. streptomycin, benzylpenicillin, carbenicillin and phenoxymethylpenicillin decreased the activity of the above dehydrogenases. tetracycline and oxytetracycline activated lactate dehydrogenase and alcohol dehydrogenase in the membr ... | 1978 | 713882 |
| microbiological determination of zinc bacitracin in animal feedingstuffs. | 1978 | 717788 | |
| the positional specificity of a desaturase in the psychrophilic bacterium micrococcus cryophilus (atcc 15174). | the positional specificity of the desaturase activity in the psychrophilic bacterium micrococcus cryophilus (atcc 15174) is shown to be delta9. the desaturase is inhibited by sterculic acid. small amounts of delta8, delta10 and delta11 isomers are present. the implications of these findings for fatty acid metabolism in m. cryophilus are discussed. it is suggested that the temperature-dependent chain length change, known to occur in the phospholipid fatty acids of this bacterium, is not mediated ... | 1978 | 718969 |
| evidence for alteration of the membrane-bound ribosomes in micrococcus luteus cells exposed to lead. | micrococcus luteus cell exposed to pb(no3)2 contained cytosol ribosomal particles and disaggregated membranal ribosomal particles as determined by ultracentirifugation and spectral studies. approx. 60% of the membrane ribosome fraction from lead exposed cells had a sedimentation value of 8.4s. cytosol ribosomes from lead exposed cells as well as membranal and cytosol ribosomes from control cells were comparable by their contents of predominantly the 70s type with the 50s and 100s present in rela ... | 1978 | 719816 |
| process of consecutive cell divisions and separations in a regular tetrads-forming mutant of micrococcus lysodeikticus (luteus). | a mutant mt of micrococcus lysodeikticus (luteus) ifo 3333, whose minimum growing unit is not a single cell, but a tetrad unlike the wild-type divides by binary fission of each monococcus, and then separates first into two daughter tetrads, second into four tetrads and third into eight tetrads. the three planes of either the cell division or the cell separation are equivalent to one another and oriented at right angles in three dimensions, respectively. the process of consecutive cell divisions ... | 1978 | 723650 |
| site of cellular autolysis in micrococcus lysodeikticus (luteus) as seen by electron microscopy. | 1978 | 723652 | |
| [effect of chlorophyllipt on the biochemical activity of staphylococci and micrococci]. | 1978 | 723658 | |
| effect of group specific reagents on the mg2 +/- dependent activity of purified micrococcus lysodeikticus atpase. | a series of group specific reagents has been examined for their ability to inactivate micrococcus lysodeikticus adenosine triphosphatase assayed with mg2+ as activating divalent cation. the enzyme activity was not inhibited by sulphydryl, carboxyl, histidine, arginine and methionine specific reagents at inhibitor concentrations below 2 mm. however, the atpase was inactivated by its chemical reaction with either one molecule of trinitrobenzenesulfonic acid or tetranitromethane, or two to four mol ... | 1978 | 725231 |
| effects of binding and bactericidal action of vancomycin on bacillus licheniformis cell wall organization as probed by 15n nuclear magnetic resonance spectroscopy. | the effects of binding and the bactericidal action of vancomycin on the arrangement and mobilities of cell wall polymers in bacillus licheniformis were investigated by (15)n nuclear magnetic resonance spectroscopy. the bactericidal action of vancomycin led to reduced mobilities of cell wall teichoic acid and teichuronic acid in surviving cells. the decrease in teichoic acid mobility was also observed upon binding of vancomycin to b. licheniformis cells and resulted from a specific interaction be ... | 1978 | 727760 |
| influence of micrococcus, bcg and related polysaccharides on the proliferation of the l1210 leukaemia. | a comparative study of the effects of bcg, micrococcus lysodeikticus, and a series of structurally related polysaccharides (complement triggers) on the non-specific and specific immune resistance against l1210 lymphoid leukaemia was carried out and commented on. in contrast with authors of earlier reports, we were unable to generate any effective non-specific or specific immunotherapy after the graft of 10(4) leukaemic cells to 8--10-week-old cdf1 mice. however, when mice were prevaccinated with ... | 1978 | 728349 |
| well-defined insoluble primers for the enzymatic synthesis of oligo- and polynucleotides. | two methods are described by which primer molecules like upu and oligodeoxythymidylates can be coupled with high efficiency to an insoluble polymer, like hydroxypropylated sephadex g-50, by one covalent linkage. in one procedure aliphatic dicarboxylic dichlorides (e.g. adipoyl dichloride) are used to serve as spacers of variable length and for anchoring the primer molecule upu. the other method involves pu as an anchor for (pdt)3 and (pdt)6, which are coupled to the polymer using condensation re ... | 1978 | 730115 |
| effect of antibiotics, non-ionic detergents, and vitamins on the osmotic barriers of micrococcus glutamicus. | the production of amino acids by the mutant strain, homoserine methionine deficient, of micrococcus glutamicus, was studied through the elucidation of the role of chemical agent affecting the osmotic barriers. penicillin was found to affect the cell wall integrity and to increase greatly the total amino acid content, especially in presence of high biotin content. non-ionic detergents were found to affect the integrity of cytoplasmic membrane. the effect was not similar with the different types o ... | 1978 | 735506 |
| a new prenyltransferase from micrococcus lysodeikticus. | 1978 | 736921 | |
| tumour immunoprophylaxis exerted by the antimicrococcus immunity. i. influence on the proliferation of murine leukaemic l1210 cells. | 1978 | 738326 | |
| tumour immunoprophylaxis exerted by the antimicrococcus immunity. ii. influence on the proliferation of murine plasmacytoma (mopc 176) and ehrlich carcinoma cells. | 1978 | 738337 | |
| staphylococcus saprophyticus: a frequent cause of acute urinary tract infection among female outpatients. | the frequency of staphylococcus saprophyticus, formerly micrococcus subgroup 3, as the cause of bacteriuria is reported. of 787 consecutive female outpatients with signs of bacteriuria, 173 (22.0%) yielded s. saprophyticus, predominantly in pure culture and in high counts. in the age group 16--25 years, this organism was demonstrated in no less than 42.3%. in hospitalized women and in men with signs of bacteriuria, on the other hand, s. saprophyticus was a rare finding (0.9% and 0.5%, respective ... | 1978 | 739158 |
| effects of chloramphenicol on the postreplication repair and sister recombinational dna exchanges in ultraviolet-irradiated micrococcus luteus. | the filling of about one third of postreplication dna gaps in u.v.-irradiated micrococcus luteus atcc 4698 is blocked by chloramphenicol (ca) added just before irradiation. addition of ca 15 min after u.v.-irradiation does not prevent the complete repair of the gaps. u.v.-sensitive m. luteus mutants (ml 6 and ml 15) are identified as defective in different steps of inducible postreplication dna repair (prr). prr in unexcising m. luteus strain g7 is accompanied by the transfer of about 20% of pyr ... | 1978 | 739981 |
| [has the respiratory chain of micrococcus lysodeiktocus any redox components on the outer surface of cytomembrane?]. | m. lysodeikticus protoplasts have catalyzed the reduction of 5.10(-4) m ferricianide by endogenous substrates if the respiratory chain is inhibited by cyanide or anaerobiosis. a disturbance of the protoplast permeability by osmotic shock or triton x-100 treatment resulted in the decrease of the endogenous ferricianide reduction rate and in simultaneous stimulation of malate ferricianide reductase activity in dehydrogenase site of the inner membrane surface. reactivation of endogenous ferricianid ... | 1978 | 743510 |
| the relevance of bacterial lytic activity in the taxonomy of the micrococcaceae: failure of its production by micrococcus and planococcus as opposed to staphylococcus. | 1978 | 745006 | |
| [deuterated membranes of micrococcus lysodeikticus: production and several biochemical properties]. | a technique has been elaborated for preparation of deuterated membranes from deuterated cells of micrococcus lysodeikticus containing 85--90% of deuterium according to the data of ir and pmr spectroscopy. normal lysis of the deuterated cells of m. lysodeikticus requires a concentration of lysozyme which is eight times higher than for usual cells (8 mg per 1 g of wet deuterated cells) and an addition of the lytic enzymes e-2 (2 mg per 1 g of wet deuterated cells). preparations of deuterated membr ... | 1978 | 745557 |
| isolation of glucosamine 6-phosphate from the cell walls of micrococcus lysodeikticus. | 1978 | 749909 | |
| patterns of cell division, dna base compositions, and fine structures of some radiation-resistant vegetative bacteria found in food. | representative highly radiation-resistant moraxella-acinetobacter (m-a), pseudomonas radiora, micrococcus radiodurans, and micrococcus radiophilus exhibited a wide variety of division systems and cell wall characteristics. however, the most resistant m-a possessed unusually thick cell walls, indicating a possible role of the cell wall in radiation resistance in the m-a. thick septation was present in most of the bacteria studied, but was absent in p. radiora, thus excluding this as a necessity f ... | 1979 | 760633 |
| biosynthesis of the linkage unit joining peptidoglycan to poly(n-acetylglucosamine 1-phosphate) in walls of micrococcus varians atcc 29750. | 1979 | 761625 | |
| phosphate transport in arsenate-resistant mutants of micrococcus lysodeikticus. | two types of arsenate-resistant mutants of micrococcus lysodeikticus were found: (i) mutants that grow in the presence of 10 mm but not 1 mm phosphate (pi) with low uptake rate for pi and arsenate, and (ii) mutants able to grow in the presence of 10 mm and 1 mm pi, with a near-normal uptake rate for pi but a low one for arsenate. the km values for pi transport and the ki values for its competitive inhibition by arsenate were similar for the mutants and the wild type. similar to the wild type, th ... | 1979 | 762027 |