Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| exopolysaccharide-producing lactic acid bacteria strains from traditional thai fermented foods: isolation, identification and exopolysaccharide characterization. | lactic acid bacteria (lab) isolated from various traditional thai fermented foods were screened for exopolysaccharides (eps) production. from 104 isolates, two rod-shaped and five coccal-shaped lab were able to produce eps from sucrose on solid media. however, only the cocci were capable of producing eps in liquid media and these were identified as pediococcus pentosaceus. pediococcus pentosaceus strains ap-1 and ap-3 produced eps in high yield. in liquid media containing sucrose as carbon sourc ... | 1999 | 10574086 |
| prolonging cell-free protein synthesis with a novel atp regeneration system. | a new approach for the regeneration of adenosine triphosphate (atp) during cell-free protein synthesis was developed to prolong the synthesis and also to avoid the accumulation of inorganic phosphate. this approach was demonstrated in a batch system derived from escherichia coli. contrary to the conventional methods in which exogenous energy sources contain high-energy phosphate bonds, the new system was designed to generate continuously the required high-energy phosphate bonds within the reacti ... | 1999 | 10577472 |
| in vitro activities of ketolide hmr3647, macrolides, and other antibiotics against lactobacillus, leuconostoc, and pediococcus isolates. | testing of susceptibility to 13 antibiotics was performed with 90 isolates of lactobacillus, leuconostoc, and pediococcus. mics at which 90% of the isolates tested were inhibited by hmr3647, erythromycin, and ciprofloxacin were 0.015, 0.125 and 32 microg/ml, respectively. the penicillin mic was > or = 16 microg/ml against 26.2% of the studied lactobacillus sp. isolates and 50% of lactobacillus plantarum. hmr3647 showed excellent activity against these genera. | 1999 | 10582908 |
| effect of ultrafiltration of yeast extracts on their ability to promote lactic acid bacteria growth. | five yeast extracts (ye) were fractionated by ultrafiltration (uf) with 1, 3, and 10 kda molecular weight cutoff membranes, concentrated by freeze-drying, and the resulting powders of yeast extract filtrates (yef) were evaluated for their growth-promoting properties on nine cultures of lactic acid bacteria (lab). there was an increase in alpha-amino nitrogen content of the yef powders as the pore size of the uf membranes used to filter the ye solutions decreased. the source of ye had a much grea ... | 1999 | 10588041 |
| nucleotide sequence and analysis of plasmid pmd136 from pediococcus pentosaceus fbb61 (atcc43200) involved in pediocin a production. | the complete sequence of the 19515-bp plasmid pmd136 from pediococcus pentosaceus fbb61 (atcc43200) has been determined. this plasmid is involved in pediocin a production, a bacteriocin active against a wide range of gram-positive bacteria. it appears to replicate via a theta mechanism, with structures closely related to those of many lactococcal plasmids. genes homologous to mobilization functions are also present, which are similar in sequence and arrangement to moba, mobb, and mobc of some st ... | 2000 | 10686129 |
| characterization and determination of origin of lactic acid bacteria from a sorghum-based fermented weaning food by analysis of soluble proteins and amplified fragment length polymorphism fingerprinting. | the group that includes the lactic acid bacteria is one of the most diverse groups of bacteria known, and these organisms have been characterized extensively by using different techniques. in this study, 180 lactic acid bacterial strains isolated from sorghum powder (44 strains) and from corresponding fermented (93 strains) and cooked fermented (43 strains) porridge samples that were prepared in 15 households were characterized by using biochemical and physiological methods, as well as by analyz ... | 2000 | 10698775 |
| production of african breadfruit (treculia africana) and soybean (glycine max) seed based food formulations, 2: effects of germination and fermentation on microbiological and physical properties. | the effects of germination (g) and naturally fermented (f) on the bacterial flora, viscosities and moisture sorption isotherms of soybean (s) and african breadfruit (b) seed based food products were investigated. bacillus, enterobacter, enterobacteriaceae, proteus, serratia and staphylococcus species dominated in the nonfermented products. lactobacillus, leuconostoc, pediococcus and yeast species dominated in the fermented products whose gruels also inhibited growth of coagulase positive staphyl ... | 1999 | 10716402 |
| enterococcal-type glycopeptide resistance genes in non-enterococcal organisms. | although the emergence of vancomycin-resistant enterococci can be attributed, in part, to the increasing use of vancomycin in clinical practice, and glycopeptide use in animal husbandry, the origins of the enterococcal vancomycin resistance genes are not clear. the vancomycin resistance-associated genes in enterococcus gallinarum, enterococcus casseliflavus/flavescens, lactobacillus spp., leuconostoc spp., pediococcus spp., and erysipelothrix rhusiopathiae, are not the source of the high-level v ... | 2000 | 10731599 |
| expanded bed adsorption as a unique unit operation for the isolation of bacteriocins from fermentation media. | expanded bed adsorption using a strong cation exchanger allowed the direct isolation of amylovorin l471, a bacteriocin from lactobacillus amylovorus dce 471, from the fermentation medium. the ph of the loading and elution buffer were optimised in a packed bed with cell-free culture supernatant. bound bacteriocin was eluted with 1.0 m nacl. the highest recovery (30%) was obtained at the lowest ph (3.6). at higher ph values the recovery was lower, namely 12%, 15% and 7% at ph 4.5, 6.5 and 8.0, res ... | 1999 | 10734568 |
| bacteriocin production by lactic acid bacteria isolated from rioja red wines. | forty-two lactic acid bacteria (lab) of the genera lactobacillus (32), leuconostoc (6), pediococcus (3) and lactococcus (1), isolated from rioja red wines, were tested for antimicrobial activity. all these strains, as well as 18 leuconostoc oenos and 19 yeast strains were used as indicators. only nine strains showed antimicrobial activity, and all were of the species lactobacillus plantarum, which constitutes the predominant microflora in rioja red wines after alcoholic fermentation. lact. plant ... | 2000 | 10735242 |
| effect of some nutritional and environmental parameters on the production of diacetyl and on starch consumption by pediococcus pentosaceus and lactobacillus acidophilus in submerged cultures. | three series of 5-day submerged cultures with pediococcus pentosaceus mitj-10 and lactobacillus acidophilus hansen 1748 were carried out in starch-based media, and the effect of cultural factors on the changes of starch, diacetyl and amylase activity determined. in axenic cultures, ped. pentosaceus mitj-10 produced more diacetyl (63.27 mg l(-1)) by adding glucose, yeast extract and caco3 (p < 0.01), at 28 degrees c (p < 0.05); but more starch was consumed (18.4 g l(-1)) in the absence of glucose ... | 2000 | 10735253 |
| identification of pediococci by ribotyping. | pediococci are among the most prevalent microbial contaminants in breweries and they can cause ropiness and the accumulation of high levels of diacetyl in beer. the accurate identification of pediococci is important, because different species do not possess equal spoilage potential. in this study, 18 pediococcus strains, mainly of brewery origin, were first identified using phenotypical characterization (api 50 chl and sds-page profiling), and then ribotyped using a riboprinterr system. six pedi ... | 2000 | 10735994 |
| identification and enumeration of oleic acid and linoleic acid hydrating bacteria in the rumen of sheep and cows. | the diversity and population densities of facultative anaerobic bacteria with the capacity to hydrate oleic acid and linoleic acid in the rumen of sheep and dairy cows were determined. the screening of representative colonies, from rumen fluid plated aerobically on a range of agar media, revealed that sheep rumen fluid contained hydration-positive strains of streptococcus, staphylococcus, enterococcus, lactobacillus and pediococcus, whereas cow rumen fluid contained hydration-positive strains of ... | 2000 | 10735997 |
| enterocin 012, a bacteriocin produced by enterococcus gallinarum isolated from the intestinal tract of ostrich. | enterococcus gallinarum strain 012, isolated from the duodenum of ostrich, produced enterocin 012 which is active against ent. faecalis, lactobacillus acidophilus, lact. sake, listeria innocua, propionibacterium acidipropionici, propionibacterium sp., clostridium perfringens, pseudomonas aeruginosa and salmonella typhimurium. one of the four pathogenic strains of escherichia coli isolated from the intestinal tract of ostrich was inhibited by enterocin 012. no antimicrobial activity was recorded ... | 2000 | 10736005 |
| cloning and expression of a plasmid-linked pediocin determinant trait of pediococcus acidilactici f. | plasmid dna from pediococcus acidilactici f was prepared by lysozyme-mutanolysin method and purified by cesium chloride-ethidium bromide (cscl-etbr) density gradient ultracentrifugation. agarose gel electrophoresis of plasmid dna and plasmid-curing experiments suggested that bacteriocin activity was harboured on a small plasmid of approximately 9.1 kb (kilobasepair) in pediococcus acidilactici f. plasmid encoding bacteriocin production in p. acidilactici f was examined for restriction enzyme cle ... | 2000 | 10746198 |
| lactobacillus farciminis md, a newer strain with potential for bacteriocin and antibiotic assay. | a native isolate lactobacillus farciminis md isolated from fermenting mushroom exhibited a high degree of sensitivity to the majority of the bacteriocins produced by strains of lactobacilli, leuconostoc and pediococci. also, the efficacy of lact. farciminis md as a sensitive strain for antibiotic assay was established against different antibiotics including ampicillin, cefazoline, chloramphenicol and nitrofurantoin at concentrations of 30 microg each, showing an inhibition zone of 30 mm diameter ... | 2000 | 10747250 |
| use of a single, triplicate arbitrarily primed-pcr procedure for molecular fingerprinting of lactic acid bacteria. | arbitrarily primed (ap)-pcr can be used to generate characteristic dna fingerprint patterns. however, small changes in reaction conditions can cause band irreproducibility. in this study, a single methodology encompassing triplicate reactions, which were intentionally exposed to three different annealing temperatures, enabled bands that were reproducibly generated to be recognized. a single triplicate ap-pcr (tap-pcr) procedure, using an 18-mer primer, was developed and used to fingerprint repre ... | 2000 | 10788406 |
| synergistic lethal combination of nitrite and acid ph on a verotoxin-negative strain of escherichia coli o157. | this study was concerned with the possible consequences of reducing the nitrite concentration of a fermented sausage environment on the survival of the pathogen e. coli o157:h45, a verotoxin-negative relative of e. coli o157:h7. a liquid medium, fm, was constructed with a liquid phase, a(w) and ph similar to fermented sausage. survival of e. coli o157:h45 in fm depended on both ph and nitrite concentration. in trials in which the ph was decreased by growing pediococcus acidilactici in fm, surviv ... | 2000 | 10791753 |
| induction and characterization of pediococcus acidilactici temperate bacteriophage | mitomycin c was used to induce temperate bacteriophage from three strains of pediococcus acidilactici. the new bacteriophage, designated pa97, pa40, and pa42, were characterized based on morphology, dna homology, and major protein profiles. morphological attributes (small isometric heads with non-contractile tails) place these bacteriophages within the b1 group of the family siphovirdae. restriction endonuclease digests suggested that the bacteriophage genomes were linear molecules without cohes ... | 1999 | 10794138 |
| description of paralactobacillus selangorensis gen. nov., sp. nov., a new lactic acid bacterium isolated from chili bo, a malaysian food ingredient. | paralactobacillus selangorensis gen. nov., sp. nov. is described. this organism, isolated from a malaysian food ingredient called chili bo, is an obligatory homofermentative, rod-shaped lactic acid bacterium. the g+c content is 46.1-46.2+/-0.3 mol%. earlier 16s rrna studies showed that this organism constitutes a new taxon distantly related to the lactobacillus casei-pediococcus group. a phenotypic description that distinguishes paralactobacillus selangorensis from other genera of lactic acid ba ... | 2000 | 10826783 |
| characterization of a phage resistance plasmid, plks, of silage-making lactobacillus plantarum ngri0101. | phage contamination has resulted in abnormal fermentation in silage. we isolated a phage-resistant strain, lactobacillus plantarum ngri0101 from silage. the strain carried two plasmids, plkl (6.8 kb) and plks (2.0 kb). by curing and retransformation of the plasmids, we clarified that plks has phage resistant activity, characterized as no adsorption inhibition. plks has 2,025 bp and three orfs, orfl23, orf132, and orf918. the predicted amino acid sequence of the orf918 product showed high similar ... | 2000 | 10830488 |
| purification, characterization, and primary structure of a novel cell wall hydrolytic amidase, cwha, from achromobacter lyticus. | a novel bacteriolytic enzyme cwha (cell wall hydrolytic amidase) was purified by ion exchange and gel-filtration chromatographies from a commercial bacteriolytic preparation from achromobacter lyticus. cwha exhibited optimal ph at 8.5 and lysed chcl(3)-treated escherichia coli more efficiently than micrococcus luteus, staphylococcus aureus, enterococcus faecalis, and pediococcus acidilactici. the enzyme was inhibited by 1,10-phenanthroline strongly and by edta to a lesser extent, suggesting that ... | 2000 | 10833271 |
| lactobacillus algidus sp. nov., a psychrophilic lactic acid bacterium isolated from vacuum-packaged refrigerated beef. | lactobacillus algidus sp. nov. is described on the basis of 40 strains isolated as one of the predominant bacteria from five specimens of vacuum-packaged beef collected from different meat shops and stored at 2 degrees c for 3 weeks. these strains were quite uniform in the overall characteristics examined. they are facultatively anaerobic, psychrophilic, gram-positive, non-spore-forming, non-motile, lactic acid-homofermentative rods. the cells occurred singly and in pairs on agar media and in ra ... | 2000 | 10843056 |
| differentiation of tetragenococcus populations occurring in products and manufacturing processes of puffer fish ovaries fermented with rice-bran. | tetragenococcus strains isolated from the manufacturing process of japanese puffer fish ovaries fermented with rice-bran were characterized and differentiated phenotypically and genotypically. a total of 413 tetragenococcus isolates were evaluated. on the basis of five representative substrates, the isolates were grouped into seven groups. an rflp (restriction fragment length polymorphisms) analysis of the 16s rrna gene of representative strains of major groups revealed that they could be groupe ... | 2000 | 10857547 |
| enzyme, bacterial inoculant, and formic acid effects on silage composition of orchardgrass and alfalfa. | we evaluated the effects of cellulase (from trichoderma longibrachiatum) application rates on neutral detergent fiber (ndf) concentration and fermentation products of orchardgrass (dactylis glomerata l.) and alfalfa (medicago sativa l.) silages harvested with decreasing dry matter (dm) digestibility. additionally, the impacts of inoculant (lactobacillus plantarum and pediococcus cerevisiae), pectinase (from aspergillus niger), or formic acid on silage composition were studied. forages wilted to ... | 2000 | 10908057 |
| heterologous coproduction of enterocin a and pediocin pa-1 by lactococcus lactis: detection by specific peptide-directed antibodies. | antibodies against enterocin a were obtained by immunization of rabbits with synthetic peptides ph4 and ph5 designed, respectively, on the n- and c-terminal amino acid sequences of enterocin a and conjugated to the carrier protein klh. anti-ph4-klh antibodies not only recognized enterocin a but also pediocin pa-1, enterocin p, and sakacin a, three bacteriocins which share the n-terminal class iia consensus motif (ygngvxc) that is contained in the sequence of the peptide ph4. in contrast, anti-ph ... | 2000 | 10919819 |
| isolation and identification of tetracycline resistant lactic acid bacteria from pre-packed sliced meat products. | in recent years, the food chain has been recognised as one of the main routes for transmission of antibiotic resistant bacteria between the animal and human population. in this regard, the current study aimed to investigate if tetracycline resistant (tetr) lactic acid bacteria (lab) are present in ready-to-eat modified atmosphere packed (map) sliced meat products including fermented dry sausage, cooked chicken breast meat and cooked ham. from population graphs based on doubling tetracycline conc ... | 2000 | 10930081 |
| molecular diversity of lactic acid bacteria from cassava sour starch (colombia). | lactic acid bacteria and more particularly lactobacilli and leuconostoc, are widely found in a wide variety of traditional fermented foods of tropical countries, made with cereals, tubers, meat or fish. these products represent a source of bacterial diversity that cannot be accurately analysed using classical phenotypic and biochemical tests. in the present work, the identification and the molecular diversity of lactic acid bacteria isolated from cassava sour starch fermentation were assessed by ... | 2000 | 10930082 |
| genomic subpopulations within the species pediococcus acidilactici detected by multilocus typing analysis: relationships between pediocin ach/pa-1 producing and non-producing strains. | a high degree of genetic polymorphism among p. acidilactici strains was highlighted by a multilocus typing approach analysing several housekeeping genes and by sampling the whole genome using random amplified polymorphic dna (rapd) fingerprint analysis performed by using a single primer peda gene targeted in low-stringency amplification conditions. restriction fragment length polymorphism of the rpoc, ldhd/l and mle genes, and a modified rapd analysis, permitted the grouping of pediococcus acidi ... | 2000 | 10931907 |
| solvent extraction of bacteriocins from liquid cultures. | a solvent extraction method was developed to concentrate lacidin from the culture of lactobacillus acidophilus osu133. the new method concentrates the bacteriocin at the interface between chloroform and the aqueous culture of the producing bacterium. compared with other extraction procedures, the new method effectively recovers higher bacteriocin yield and results in relatively clean preparations. recovery of lacidin by the chloroform extraction procedure, compared with ammonium sulphate precipi ... | 2000 | 10972727 |
| analysis of the genetic determinant for production of the pediocin p of pediococcus pentosaceus pep1. | pediococcus pentosaceus pep1 is a vacuum-packaged turkish sausage isolate which produces a potentially novel bacteriocin of the pediocin (anti-listeria) family of peptides designated as pediocin p. curing experiments and plasmid profile analysis indicated that both bacteriocin immunity and production determinants were linked and encoded by 9.0 mda plasmid, phd1.0. attempts to transform purified plasmid phd1.0 into recipient escherichia coli jm109 cells by electroporation were successful but none ... | 2000 | 10986669 |
| acetaldehyde metabolism by wine lactic acid bacteria. | acetaldehyde is a volatile flavor compound present in many fermented foods and is important in the production of red and white wines. nine strains of the genera lactobacillus and oenococcus were able to metabolize acetaldehyde in a resting cell system, whereas two pediococcus strains were not. acetic acid and ethanol were produced from its degradation. a lactobacillus and an oenococcus were able to degrade so(2)-bound acetaldehyde, as well. a coincubation of resting cells of saccharomyces bayanu ... | 2000 | 11004399 |
| engineering increased stability in the antimicrobial peptide pediocin pa-1. | pediocin pa-1 is a food grade antimicrobial peptide that has been used as a food preservative. upon storage at 4 degrees c or room temperature, pediocin pa-1 looses activity, and there is a concomitant 16-da increase in the molecular mass. it is shown that the loss of activity follows first-order kinetics and that the instability can be prevented by replacing the single methionine residue (met31) in pediocin pa-1. replacing met by ala, ile, or leu protected the peptide from oxidation and had onl ... | 2000 | 11055926 |
| identification of enterococcus species and phenotypically similar lactococcus and vagococcus species by reverse checkerboard hybridization to chaperonin 60 gene sequences. | data from four recent studies (s. h. goh et al., j. clin. microbiol. 36:2164-2166, 1998; s. h. goh et al., j. clin. microbiol. 34:818-823, 1996; s. h. goh et al., j. clin. microbiol. 35:3116-3121, 1997; a. y. c. kwok et al., int. j. syst. bacteriol. 49:1181-1192, 1999) suggest that an approximately 600-bp region of the chaperonin 60 (cpn60) gene, amplified by pcr with a single pair of degenerate primers, has utility as a potentially universal target for bacterial identification (id). this cpn60 ... | 2000 | 11060051 |
| intake, digestibility, and composition of orchardgrass and alfalfa silages treated with cellulase, inoculant, and formic acid fed to lambs. | the objectives of this study were to determine the effect of a cellulase (from trichoderma longibrachiatum) alone or combined with a bacterial inoculant (lactobacillus plantarum and pediococcus cerevisiae) or formic acid on composition, intake, and digestibility of orchardgrass (dactylis glomerata l.) and alfalfa (medicago sativa l.) silages. orchardgrass and alfalfa were harvested at the early heading stage and at the early bloom stage of maturity and wilted to approximately 22 and 32% dm, resp ... | 2000 | 11063325 |
| inducible metabolism of phenolic acids in pediococcus pentosaceus is encoded by an autoregulated operon which involves a new class of negative transcriptional regulator. | pediococcus pentosaceus displays a substrate-inducible phenolic acid decarboxylase (pad) activity on p-coumaric acid. based on dna sequence homologies between the three pads previously cloned, a dna probe of the lactobacillus plantarum pdc gene was used to screen a p. pentosaceus genomic library in order to clone the corresponding gene of this bacteria. one clone detected with this probe displayed a low pad activity. subcloning of this plasmid insertion allowed us to determine the part of the in ... | 2000 | 11073918 |
| stimulation of starter culture for further reduction of foodborne pathogens during salami fermentation. | this study was conducted to determine if stimulated meat starter culture (msc; pediococcus acidilactici) would further control escherichia coli o157:h7, listeria monocytogenes, and staphylococcus aureus during salami fermentation. manganese ion (0.005% of mnso4) was used as a stimulator for the growth and acid production of msc. after 24-h salami fermentation, nonstimulated msc and stimulated msc reduced e. coli o157:h7 levels by 1.3 and 2.3 log10 units, respectively. nonstimulated msc reduced l ... | 2000 | 11079689 |
| quinupristin-dalfopristin resistance among gram-positive bacteria in taiwan. | to understand quinupristin-dalfopristin resistance among clinical isolates of gram-positive bacteria in taiwan, where this agent is not yet available for clinical use, we evaluated 1,287 nonduplicate isolates recovered from january 1996 to december 1999 for in vitro susceptibility to quinupristin-dalfopristin and other newer antimicrobial agents. all methicillin-susceptible staphylococcus aureus (mssa) isolates were susceptible to quinupristin-dalfopristin. high rates of nonsusceptibility to qui ... | 2000 | 11083643 |
| biochemical and genetic characterization of coagulin, a new antilisterial bacteriocin in the pediocin family of bacteriocins, produced by bacillus coagulans i(4). | a plasmid-linked antimicrobial peptide, named coagulin, produced by bacillus coagulans i(4) has recently been reported (b. hyronimus, c. le marrec and m. c. urdaci, j. appl. microbiol. 85:42-50, 1998). in the present study, the complete, unambiguous primary amino acid sequence of the peptide was obtained by a combination of both n-terminal sequencing of purified peptide and the complete sequence deduced from the structural gene harbored by plasmid i(4). data revealed that this peptide of 44 resi ... | 2000 | 11097892 |
| development of molecular rapd marker for the identification of pediococcus acidilactici strains. | a rapd analysis performed using a single primer targeted to the pediocin ach/pa-1 gene was carried out on several p. acidilactici strains and on some related species of lactic acid bacteria. the high degree of genetic variability detected in p. acidilactici strains did not allow the selection of a common rapd fragment that could be chosen as a potential species-specific dna marker. nevertheless a 700 bp fragment, that was found to be peculiar of all potential pediocin producer strains analyzed, ... | 2000 | 11108020 |
| riboprinting and 16s rrna gene sequencing for identification of brewery pediococcus isolates. | a total of 46 brewery and 15 atcc pediococcus isolates were ribotyped using a qualicon riboprinter. of these, 41 isolates were identified as pediococcus damnosus using ecori digestion. three atcc reference strains had patterns similar to each other and matched 17 of the brewery isolates. six other brewing isolates were similar to atcc 25249. the other 18 p. damnosus brewery isolates had unique patterns. of the remaining brewing isolates, one was identified as p. parvulus, two were identified as ... | 2001 | 11157216 |
| bacteriocin production by lactobacillus salivarius of animal origin. | 2000 | 11184178 | |
| a novel lantibiotic, nukacin isk-1, of staphylococcus warneri isk-1: cloning of the structural gene and identification of the structure. | staphylococcus warneri isk-1, which we had previously reported as pediococcus sp. isk-1, produces a novel bacteriocin, nukacin isk-1. edman degradation of the chemically reduced nukacin isk-1 produced a sequence of 27 amino acids, 7 of which were unidentified. using single-specific-primer-pcr product as a probe, a 3.6-kb hindiii fragment containing the nukacin isk-1 structural gene (nuka) was cloned and sequenced. the deduced amino acid sequence of nukacin isk-1 had 57 amino acids, including a 3 ... | 2000 | 11193411 |
| a case of septicemia with pediococcus acidilactici after long-term antibiotic treatment. | presented here is a case of septicemia caused by an uncommon, multiresistant, gram-positive microorganism (pediococcus acidilactici) after long-term antibiotic treatment. pediococcus spp. are rarely cultivated from clinical specimens, and species differentiation is difficult due to the paucity of phenotypic traits. in this case, a polyphasic approach consisting of phenotypic and molecular genetic analyses was used, and the identification of pediococcus acidilactici was conclusive. precise identi ... | 2000 | 11205633 |
| expression in escherichia coli of native and chimeric phenolic acid decarboxylases with modified enzymatic activities and method for screening recombinant e. coli strains expressing these enzymes. | four bacterial phenolic acid decarboxylases (pad) from lactobacillus plantarum, pediococcus pentosaceus, bacillus subtilis, and bacillus pumilus were expressed in escherichia coli, and their activities on p-coumaric, ferulic, and caffeic acids were compared. although these four enzymes displayed 61% amino acid sequence identity, they exhibit different activities for ferulic and caffeic acid metabolism. to elucidate the domain(s) that determines these differences, chimeric pad proteins were const ... | 2001 | 11229892 |
| [molecular structure of the locus for sucrose utilization by lactobacillus plantarum: comparison with pediococcus pentosaceus]. | structure of the sucrose utilization locus in a lactobacillus plantarum type strain was studied using pcr and southern hybridization. restriction map analysis revealed its high similarity to the sequenced sucrose utilization locus of pediococcus pentosaceus psrq1. the l. plantarum locus proved containing oppositely oriented scra and the scrbragl operon, but not agas. the l. plantarum sucrase gene (scrb) was partly sequenced. a higher (98.6%) homology was revealed between scrb than between the 16 ... | 2001 | 11234378 |
| a rapid pcr based method to distinguish between lactococcus and enterococcus. | phenotypic characterisation of lactococcus and enterococcus species remains unreliable as strains of both genera have been isolated which do not conform to the traditional criteria for separation of these genera. a bank of 131 isolates was phenotypically characterised by three methods: (a) traditional broth tests, (b) api rapid id 32 strep and (c) bbl crystal id kits. differences in genus designation between commercial kits were evident for 12 strains (9%), while 7 strains (5%) remained unidenti ... | 2000 | 11249021 |
| dry sausage fermented by lactobacillus rhamnosus strains. | the ability of three probiotic lactobacillus rhamnosus strains gg, e-97800 and lc-705 and one commercial pediococcus pentosaceus starter strain (control) to produce dry sausage was studied. during the fermentation process the numbers of inoculated lactic acid bacteria increased from approx. 7 log10 to 8-9 log10 cfu/g and the ph values decreased from 5.6 to 4.9-5.0. the sensory test indicated that the dry sausages fermented by l. rhamnosus lc-705 were inferior to the control sausages. the presenc ... | 2001 | 11252505 |
| behavior of variable v3 region from 16s rdna of lactic acid bacteria in denaturing gradient gel electrophoresis. | separation of amplified v3 region from 16s rdna by denaturing gradient gel electrophoresis (dgge) was tested as a tool for differentiation of lactic acid bacteria commonly isolated from food. variable v3 regions of 21 reference strains and 34 wild strains referred to species belonging to the genera pediococcus, enterococcus, lactococcus, lactobacillus, leuconostoc, weissella, and streptococcus were analyzed. dgge profiles obtained were species-specific for most of the cultures tested. moreover, ... | 2001 | 11270655 |
| bifunctional enzyme 6'-n-aminoglycoside acetyltransferase-2"-o-aminoglycoside phosphotransferase in lactobacillus and pediococcus isolates of animal origin. | 2001 | 11281121 | |
| phenotypic and genotypic characterization of pediococcus strains isolated from human clinical sources. | seventy-two strains of pediococci isolated from human clinical sources were characterized by conventional physiological tests, chromogenic enzymatic tests, analysis of whole-cell protein profiles (wcpp) by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and analysis of chromosomal dna restriction profiles by pulsed-field gel electrophoresis (pfge). conventional tests allowed identification of 67 isolates: 52 strains were identified as pediococcus acidilactici, 15 strains were identifi ... | 2001 | 11283035 |
| selection and characterization of mixed starter cultures for lactic acid fermentation of carrot, cabbage, beet and onion vegetable mixtures. | an evaluation of various lactic acid bacteria (lab) for the fermentation of cabbage, carrot and beet-based vegetable products was carried out. as part of a screening process, the growth of 15 cultures in a vegetable juice medium (vjm) was characterized by automated spectrophotometry. acidification patterns as well as viability during storage of the lab were also established. there were greater differences between the pure cultures than the mixed ones with respect to growth in vjm and viability d ... | 2001 | 11294348 |
| direct polymerase chain reaction detection of ropy pediococcus damnosus strains in wine. | glucan-producing strains of pediococcus damnosus are considered as spoilage micro-organisms because synthesis of glucan leads to an unacceptable viscosity of wine. in this report, we present a polymerase chain reaction (pcr) procedure to detect the presence of such strains in wines. | 2001 | 11309064 |
| flow cytometric assessment of viability of lactic acid bacteria. | the viability of lactic acid bacteria is crucial for their applications as dairy starters and as probiotics. we investigated the usefulness of flow cytometry (fcm) for viability assessment of lactic acid bacteria. the esterase substrate carboxyfluorescein diacetate (cfda) and the dye exclusion dna binding probes propidium iodide (pi) and toto-1 were tested for live/dead discrimination using a lactococcus, a streptococcus, three lactobacillus, two leuconostoc, an enterococcus, and a pediococcus s ... | 2001 | 11319119 |
| bacteremic infection with pediococcus: vancomycin-resistant opportunist. | pediococci are recently recognized gram-positive human pathogens, resistant to vancomycin and generally susceptible to penicillin. infection in adults has been seen in patients with chronic underlying conditions as well as those with previous abdominal surgery. two previous infants with congenital gastrointestinal malformations requiring surgical correction have been reported with sepsis attributable to pediococcus sp. we report a third infant born with gastroschisis who developed pediococcus ba ... | 2001 | 11335758 |
| detection of lactobacillus, pediococcus, leuconostoc, and weissella species in human feces by using group-specific pcr primers and denaturing gradient gel electrophoresis. | denaturing gradient gel electrophoresis (dgge) of dna fragments generated by pcr with 16s ribosomal dna-targeted group-specific primers was used to detect lactic acid bacteria (lab) of the genera lactobacillus, pediococcus, leuconostoc, and weissella in human feces. analysis of fecal samples of four subjects revealed individual profiles of dna fragments originating not only from species that have been described as intestinal inhabitants but also from characteristically food-associated bacteria s ... | 2001 | 11375166 |
| optimum production, stability, partial purification and inhibitory spectrum of antimicrobial compounds produced by pediococcus pentosaceus di. | the optimum conditions for production of antimicrobial compounds by pediococcus pentosaceus di were studied. the antimicrobial compounds in cell free fermented medium were extracted (methanol-acetone), then tested for inhibitory activity against fourteen bacterial strains and its stability to heating, various ph and proteolytic enzymes. in addition, methanol-acetone extract was partial purified through sephadex g-100 column and thin-layer chromatography. production of antimicrobial compounds wer ... | 2001 | 11379284 |
| the effect of lactobacillus buchneri and other additives on the fermentation and aerobic stability of barley silage. | whole-plant barley (39.4% dry matter) was treated with various chemical and biological additives to assess their effects on silage fermentation and aerobic stability. treatments were untreated forage, forage treated with several amounts of lactobacillus buchneri and enzymes (l. buchneri at 1 x 10(5), 5 x 10(5), and 1 x 10(6) cfu/g of fresh forage), forage treated with an inoculant containing (lactobacillus plantarum, pediococcus pentosaceus, propionibacterium freudenreichii, and enzymes), or for ... | 2001 | 11384041 |
| subtraction hybridization in microplates: an improved method to generate strain-specific pcr primers. | an improved subtraction hybridization technique was developed and evaluated. the hybridization is performed in a microplate with the subtractor-dna immobilized in the plate while the probe-dna is in solution. after hybridization the probe-specific dna can easily be removed from the microwell and submitted to further analysis. this new technique has been successfully applied to generate several strain-specific pcr-primers for lactococcus lactis subsp. lactis, pediococcus spec., saccharomyces spec ... | 2001 | 11403389 |
| phenotypic diversity of lactic acid bacteria isolated from fermented sausages produced in basilicata (southern italy). | to evaluate the evolution of lactic acid bacteria (lab) populations in traditional fermented sausages (salsiccia and soppressata) produced in artisanal and industrial plants in basilicata (southern italy). | 2001 | 11412324 |
| effect of physical factors on the production of bacteriocin from pediococcus acidilactici itv 26. | the effect of ph, temperature and agitation on growth and bacteriocin production by pediococcus acidilactici itv 126 was investigated. experiments were made in flasks containing mrs medium at 30 to 40 degrees c, ph 5 to 7 and agitation 0 to 200 rpm. factor levels were arranged in a 2(3) factorial design with central and axial points. anova and tukey paired comparison tests showed that a temperature of 35 degrees c favored bacteriocin production, whereas 40 degrees c was best for cell growth. a s ... | 2001 | 11464266 |
| detection and typing of vancomycin-resistance genes of enterococci from clinical and nosocomial surveillance specimens by multiplex pcr. | ninety-three clinical isolates of vancomycin-resistant enterococci (vre) collected from nine hospitals in taiwan were examined for the presence of vana, vanb, vanc1, or vanc2/vanc3 genes by a multiplex pcr. forty-seven of these vre isolates were vana positive, 1 contained both vanc1 and vana, 40 harboured vanb, 2 were vanc1, and 3 were identified to be vanc2/vanc3. twenty-four vana isolates were sensitive to teicoplanin and thus did not have a typical vana phenotype. five isolates with the vanc ... | 2001 | 11467792 |
| decrease in cell surface galactose residues of schizosaccharomyces pombe enhances its coflocculation with pediococcus damnosus. | pediococcus damnosus can coflocculate with saccharomyces cerevisiae and cause beer acidification that may or may not be desired. similar coflocculations occur with other yeasts except for schizosaccharomyces pombe which has galactose-rich cell walls. we compared coflocculation rates of s. pombe wild-type species tp4-1d, having a mannose-to-galactose ratio (man:gal) of 5 to 6 in the cell wall, with its glycosylation mutants gms1-1 (man:gal = 5:1) and gms1delta (man:gal = 1:0). these mutants coflo ... | 2001 | 11472912 |
| optimization of annealing temperature to reduce bias caused by a primer mismatch in multitemplate pcr. | to reduce pcr bias derived from a primer mismatch, the effect of the annealing temperature on the product ratio was investigated by denaturing gradient gel electrophoresis analysis of pcr products from a mixture of perfect-match and one-mismatch templates. these templates were generated by pcr from pediococcus acidilactici for one mismatch and micrococcus luteus for the perfect match. pcrs showed that the bias was reduced at lower temperatures. an environmental sample was also examined. | 2001 | 11472961 |
| comparison of pattern recognition techniques for the identification of lactic acid bacteria. | the goal of this study was to evaluate three pattern recognition methods for use in the identification of lactic acid bacteria. | 2001 | 11473587 |
| the in vitro activity of daptomycin against 514 gram-positive aerobic clinical isolates. | the in vitro activity of daptomycin was assessed in comparison with that of vancomycin and penicillin against a wide range of gram-positive aerobic clinical isolates. mics were determined by an agar dilution method on mueller-hinton agar (nccls/eucast) and on isotonic agar adjusted to contain 50 mg/l free calcium (bsac). both media were enriched with 5% horse blood for fastidious organisms. daptomycin mics for all 172 staphylococci, including methicillin-susceptible and methicillin-resistant sta ... | 2001 | 11481291 |
| flocculation and coflocculation of bacteria by yeasts. | biotransformations in natural environments frequently involve interactions between microorganisms. although there are many reports on the interactions between bacteria, interactions between yeasts and bacteria have not been extensively studied. previously we reported on the flocculation and coflocculation of pediococcus damnosus by saccharomyces cerevisiae. now we report that several other yeasts, such as candida utilis, dekkera bruxellensis, hanseniaspora guilliermondii, kloeckera apiculata, an ... | 2001 | 11525628 |
| [biological deacidification of wines using lactic-acid bacteria and yeasts]. | based on a study of 200 lactic-acid bacteria monocultures and 30 associating lactic bacteria and yeasts cultures, a stable association was created formed by leuconostoc oenos, pediococcus pentosaceus and saccharomyces cerevisiae yeasts, intended for the biological deacidification of wine. physiology of microorganisms and their effect on the wine chemical composition was studied. by means of selective association, high quality fine wines were produced from the high-acid wines. | 2001 | 11530675 |
| characterization, production, and purification of carnocin h, a bacteriocin produced by carnobacterium 377. | carnocin h, a bacteriocin produced by a carnobacterium sp., inhibited lactic acid bacteria, clostridia, enterococci, and some staphylococcus aureus strains. some strains of listeria and pediococcus were also sensitive to carnocin h. the bacteriocin was produced during the late stationary growth phase. carnocin h was purified by cation exchange chromatography and reverse phase chromatography. amino acid sequence and composition indicate that carnocin h is a novel bacteriocin belonging to the clas ... | 2001 | 11683354 |
| conformational changes in pediocin ach upon vesicle binding and approximation of the membrane-bound structure in detergent micelles. | pediocin ach is a 44-residue antimicrobial peptide with bactericidal potency against gram-positive bacteria such as listeria. it belongs to a family of bacteriocins that, when membrane-associated, is predicted to contain beta-sheet and alpha-helical regions. all bacteriocins in this family have a conserved n-terminal disulfide bond. an additional c-terminal disulfide bond in pediocin ach is thought to confer enhanced potency and broader specificity range against sensitive bacteria. the c-termina ... | 2001 | 11705396 |
| lactobacillus coleohominis sp. nov., isolated from human sources. | four strains of a hitherto unrecognized gram-positive, catalase-negative, facultatively anaerobic, rod-shaped bacterium isolated from human sources were characterized using phenotypic and molecular taxonomic methods. comparative 16s rrna gene sequencing demonstrated that the bacterium represents a new subline within the lactobacillus casei/pediococcus rrna group of the genus lactobacillus. the unknown bacterium was readily distinguished from all other described lactobacillus species and related ... | 2001 | 11760950 |
| nutritional factors affecting the production of two bacteriocins from lactic acid bacteria on whey. | the ability of lactococcus lactis subsp. lactis cect 539 and pediococcus acidilactici nrrl b-5627 to produce bacteriocins on both diluted and concentrated whey was investigated in batch fermentations. both strains produced the higher amounts of biomass and bacteriocin titres on diluted whey. luedeking and piret expression was able to model the production of nisin, which was produced as a primary metabolite on both culture media. however, the pediocin production could not be typified in any case ... | 2001 | 11764192 |
| molecular diversity of lactobacillus spp. and other lactic acid bacteria in the human intestine as determined by specific amplification of 16s ribosomal dna. | a lactobacillus group-specific pcr primer, s-g-lab-0677-a-a-17, was developed to selectively amplify 16s ribosomal dna (rdna) from lactobacilli and related lactic acid bacteria, including members of the genera leuconostoc, pediococcus, and weissella: amplicons generated by pcr from a variety of gastrointestinal (gi) tract samples, including those originating from feces and cecum, resulted predominantly in lactobacillus-like sequences, of which ca. 28% were most similar to the 16s rdna of lactoba ... | 2002 | 11772617 |
| luminescent method for the detection of antibacterial activities. | a new rapid and sensitive method for the detection of antibacterial activities was based on luminescent indicator strains. listeria innocua 8811 and enterococcus faecalis 32 were transformed with plasmid carrying bacterial luciferase genes. subsequent strains became capable to emit light during the exponential growth phase. the addition of bacteriocin containing culture supernatants to such cultures induced a drop of their light emission which was correlated to the combined antibacterial activit ... | 2001 | 11778890 |
| enterococcal diversity in the environment of an irish cheddar-type cheesemaking factory. | enterococci are natural residents of human and animal intestinal tracts and grow to high levels in a variety of artisanal cheeses. the aim of this study was to determine the diversity of enterococci in a farmhouse raw-milk cheese production unit. putative enterococci were isolated from the faeces of all the cows and all the people associated with the cheesemaking, from the milk and cheese during manufacture and ripening and from the environment in three separate trials. almost 1400 isolates were ... | 2001 | 11789936 |
| efficient protoplast regeneration for some homofermentative lactobacilli and pediococci. | conditions for protoplast regeneration were examined for several strains of homofermentative lactobacilli and pediococci isolated from silage. attempts to regenerate protoplasts using previously published agar regeneration media for lactobacilli were unsuccessful for most of the strains. replacing or increasing colloidal substances in a medium containing raffinose and mgcl(2) as osmotic stabilizers enabled efficient regeneration of the protoplasts at a frequency of 10-99%. a medium containing ge ... | 2001 | 11797042 |
| multilocus hybridization typing in pediococcus acidilactici strains. | in previous a study we demonstrated the presence of several genomic subpopulations within a collection of pediococcus acidilactici strains isolated from different environments, through a multilocus typing analysis taking into consideration housekeeping conserved loci and protein coding genes of the primary metabolism. in this study, representative strains of five genomic subpopulations previously described (i, ii, iii, v, vii) were analyzed by restriction analysis of chromosomal dna and subseque ... | 2002 | 11815849 |
| cloning of the l-lactate dehydrogenase gene from the ruminal bacterium selenomonas ruminantium hd4. | a clone from a selenomonas ruminantium hd4 lambda zap ii genomic library was isolated by its ability to complement the anaerobic growth deficiency of an escherichia coli (pfl, ldh) double mutant. the 1.0-kb insert from the clone was sequenced and revealed a single open reading frame (orf, 957-bp) which was preceded by a putative shine-dalgarno (sd) sequence (aggggg). the potential sd sequence corresponded to 3' 16s rrna sequences of various selenomonas strains. the orf was predicted to encode a ... | 2002 | 11821921 |
| characterisation of is153, an is3-family insertion sequence isolated from lactobacillus sanfranciscensis and its use for strain differentiation. | an insertion sequence has been identified in the genome of lactobacillus sanfranciscensis dsm 20451t as segment of 1351 nucleotides containing 37-bp imperfect terminal inverted repeats. the sequence of this element encodes two out of phase, overlapping open reading frames, orfa and orfb, from which three putative proteins are produced. orfab is a transframe protein produced by -1 translational frame shifting between orf a and orf b that is presumed to be the transposase. the large orfab of this ... | 2001 | 11822682 |
| genomic diversity within the genus pediococcus as revealed by randomly amplified polymorphic dna pcr and pulsed-field gel electrophoresis. | the genomic diversity of 33 previously assigned strains from six species within the genus pediococcus was assessed by randomly amplified polymorphic dna (rapd) pcr and pulsed-field-gel electrophoresis (pfge). the rapd pcr patterns produced by two separate random primers, termed p1 (acgcgccct) and p2 (atgtaacgcc), were compared by the pearson correlation coefficient and the unweighted pair group method with arithmetic averages clustering algorithm. pattern variations between repeat samples set a ... | 2002 | 11823217 |
| rapid two-step procedure for large-scale purification of pediocin-like bacteriocins and other cationic antimicrobial peptides from complex culture medium. | a rapid and simple two-step procedure suitable for both small- and large-scale purification of pediocin-like bacteriocins and other cationic peptides has been developed. in the first step, the bacterial culture was applied directly on a cation-exchange column (1-ml cation exchanger per 100-ml cell culture). bacteria and anionic compounds passed through the column, and cationic bacteriocins were subsequently eluted with 1 m nacl. in the second step, the bacteriocin fraction was applied on a low-p ... | 2002 | 11823243 |
| competitive inhibition of listeria monocytogenes in ready-to-eat meat products by lactic acid bacteria. | forty-nine strains of lactic acid bacteria (lab), isolated from commercially available ready-to-eat (rte) meat products, were screened for their ability to inhibit the growth of listeria monocytogenes at refrigeration (5 degrees c) temperatures on agar spot tests. the three most inhibitory strains were identified as pediococcus acidilactici, lactobacillus casei, and lactobacillus paracasei by 16s rdna sequence analysis. their antilisterial activity was quantified in associative cultures in deman ... | 2002 | 11848562 |
| nisin and pediocin production on mussel-processing waste supplemented with glucose and five nitrogen sources. | optimization of bacteriocin production by l. lactis subsp. lactis cect 539 and ped. acidilactici nrrl b-5627 on mussel-processing wastes. | 2002 | 11849506 |
| the gram-positive tonsillar and nasal flora of piglets before and after weaning. | to investigate gram-positive nasal and tonsillar microbial flora of piglets before and after weaning. | 2001 | 11851806 |
| growth optimization of pediococcus damnosus ncfb 1832 and the influence of ph and nutrients on the production of pediocin pd-1. | optimization of the growth of pediococcus damnosus ncfb 1832 and the production of pediocin pd-1 by traditional fermentation methods. | 2001 | 11851822 |
| fermentation and microflora of plaa-som, a thai fermented fish product prepared with different salt concentrations. | plaa-som is a thai fermented fish product prepared from snakehead fish, salt, palm syrup and sometimes roasted rice. we studied the effects of different salt concentrations on decrease in ph and on microflora composition during fermentation. two low-salt batches were prepared, containing 6% and 7% salt (w/w) as well as two high-salt batches, containing 9% and 11% salt. ph decreased rapidly from 6 to 4.5 in low-salt batches, whereas in high-salt batches, a slow or no decrease in ph was found. lac ... | 2002 | 11883675 |
| in vitro and in vivo activities of a novel cephalosporin, bms-247243, against organisms other than staphylococci. | bms-247243, a novel cephalosporin inhibitory for methicillin-resistant staphylococci, primarily has activity against gram-positive bacteria. the activities of bms-247243, cefotaxime, and ceftriaxone against streptococci and streptococcus pneumoniae were similar. bms-247243 inhibits enterococcus faecalis but not enterococcus faecium. bms-247243 also inhibits many inherently vancomycin-resistant species (leuconstoc, lactobacillus, pediococcus) and anaerobic gram-positive bacteria. | 2002 | 11897599 |
| pediocin pa-1, a wide-spectrum bacteriocin from lactic acid bacteria. | pediocin pa-1 is a broad-spectrum lactic acid bacteria bacteriocin that shows a particularly strong activity against listeria monocytogenes, a foodborne pathogen of special concern among the food industries. this antimicrobial peptide is the most extensively studied class ila (or pediocin family) bacteriocin, and it has been sufficiently well characterized to be used as a food biopreservative. this review focuses on the progress that have been made in the elucidation of its structure, mode of ac ... | 2002 | 11934133 |
| growth and aroma production by staphylococcus xylosus, s. carnosus and s. equorum--a comparative study in model systems. | a laboratory medium inoculated with 20 different staphylococcus strains was prepared in accordance with a full factorial experimental design investigating the effect of temperature, ph, nacl and glucose on growth. the 12 strains most suited to growth in a fermented meat environment were inoculated in sausage minces together with pediococcus pentosaceus, incubated at 25 degrees c for 1 week and the produced aroma compounds collected. the data were analysed by multiple linear regression and partia ... | 2002 | 11999121 |
| factors affecting the adsorption of buchnericin lb, a bacteriocin produced by lactobacillus [correction of lactocobacillus] buchneri. | buchnericin-lb adsorbs to gram-positive but not to gram-negative bacteria. the tested gram-positive bacteria were species of lactobacillus, pediococcus, leuconostoc, enterococcus, lactococcus, listeria, bacillus, staphylococcus; gram-negative bacteria belonged to the genera salmonella, escherichia, yersinia and pseudomonas. buchnericin-lb adsorption depended on ph but not on time and temperature. also some anions of salts and lipoteichoic acid reduced or inhibited its adsorption. treatment of ce ... | 2002 | 12002398 |
| monitoring the identity of bacteria using their intrinsic fluorescence. | three different fluorescence spectra were recorded following excitation at 250 nm (aromatic amino acids+nucleic acids), 270 nm (tryptophan residues) and 316 nm (nadh) for 25 strains of bacteria in dilute suspensions. evaluation of the spectra using principal component analysis and hierarchical clustering showed a good reproducibility from culture to culture and a good discrimination of the bacteria. applying the method of mahalanobis distances to the spectra of lactobacilli species recorded foll ... | 2002 | 12076805 |
| persistence of escherichia coli o157:h7 in barley silage: effect of a bacterial inoculant. | the effect of a lactic acid producing bacterial (lab) inoculant on the elimination of escherichia coli o157:h7 from barley forage was assessed. | 2002 | 12147077 |
| identification of the bacterial microflora in dairy products by temporal temperature gradient gel electrophoresis. | numerous microorganisms, including bacteria, yeasts, and molds, are present in cheeses, forming a complex ecosystem. among these organisms, bacteria are responsible for most of the physicochemical and aromatic transformations that are intrinsic to the cheesemaking process. identification of the bacteria that constitute the cheese ecosystem is essential for understanding their individual contributions to cheese production. we used temporal temperature gradient gel electrophoresis (ttge) to identi ... | 2002 | 12147461 |
| apparent antifungal activity of several lactic acid bacteria against penicillium discolor is due to acetic acid in the medium. | fifty-six dairy bacteria belonging to the genera lactococcus, lactobacillus, pediococcus, propionibacterium, streptococcus, enterococcus, leuconostoc, and brevibacterium were screened for antifungal activity against four species of fungi relevant to the cheese industry (penicillium discolor, penicillium commune, penicillium roqueforti, and aspergillus vesicolor). most of the active strains belonged to the genus lactobacillus, whereas penicillium discolor was found to be the most sensitive of the ... | 2002 | 12182485 |
| the evaluation of mixtures of yeast and potato extracts in growth media for biomass production of lactic cultures. | the effectiveness of yeast extracts (ye) and potato extracts (pe) to promote growth of seven lactic cultures was evaluated by automated spectrophotometry (as). two aspects of the growth curve were analysed: (1) maximum biomass obtained (using odmax) and (2) highest specific growth rate mu(max)) eleven lots from the same pe-manufacturing process were examined for lot-to-lot variability. the odmax values of three of the seven strains were significantly affected by lot source, but mu(max) was not s ... | 2002 | 12224561 |
| production of bacteriocins from lactococcus lactis subsp. lactis cect 539 and pediococcus acidilactici nrrl b-5627 using mussel-processing wastes. | the growth and bacteriocin production by lactococcus lactis subsp. lactis cect 539 and pediococcus acidilactici nrrl b-5627 were investigated on mussel-processing wastes. both bacteriocin productions were satisfactorily modelled using a modified form of the luedeking and piret expression, which includes a term for the influence of the ph reduction rate. experimental data from cultures buffered at different initial concentrations (0, 0.03, 0.10 and 0.25 m) of both bacteria were used to fit and ve ... | 2002 | 12241553 |
| a pcr-based method for identification of lactobacilli at the genus level. | we developed a polymerase chain reaction (pcr)-based method for the identification of lactobacilli at the genus level. one specific primer, lblma1-rev, was designed by analysing similarities between the nucleotide sequence of the spacer between the 16s and 23s rrna genes in a number of lactobacillus strains. amplification with lblma1-rev and r16-1, a universal primer, generated a pcr product for 23 lactobacillus species. electrophoresis did not reveal any discrete bands when escherichia coli, la ... | 2002 | 12351242 |
| the effect of temperature on the growth and persistence of salmonella in fermented liquid pig feed. | two studies were conducted to investigate the effect of temperature on the fate of salmonella enterica subsp. enterica serovar typhimurium dt104:30 in fermented liquid pig feed. these were (1) by co-inoculation of feed with s. typhimurium dt104:30 and pediococcus pentosaceus, as the fermenting organism, and (2) by fermenting feed for 48, 72 or 96 h prior to inoculation with s. typhimurium dt104:30. in co-inoculated feed incubated at 20 degrees c, s. typhimurium dt104:30 persisted for at least 72 ... | 2002 | 12382689 |
| characterization and heterologous expression of a class iia bacteriocin, plantaricin 423 from lactobacillus plantarum 423, in saccharomyces cerevisiae. | lactobacillus plantarum 423 produces a small heat-stable antimicrobial protein designated plantaricin 423. this protein is bactericidal for many gram-positive foodborne pathogens and spoilage bacteria, including listeria spp., staphylococcus spp., pediococcus spp., lactobacillus spp., etc. the dna sequence of the plantaricin 423-encoding region on plasmid ppla4 revealed a four open reading frame (orf) operon structure similar to pediocin pa-1/ach from pediococcus acidilactici and coagulin from b ... | 2003 | 12423916 |
| microbiological and fermentation characteristics of togwa, a tanzanian fermented food. | selected microbiological and metabolic characteristics of sorghum, maize, millet and maize-sorghum togwa were investigated during natural fermentation for 24 h. the process was predominated by lactic acid bacteria (lab) and yeasts. the mesophiles, lactic acid bacteria, and yeasts increased and the enterobacteriaceae decreased to undetectable levels within 24 h. the isolated microorganisms were tentatively identified as lactobacillus plantarum, lactobacillus brevis, lactobacillus fermentum, lacto ... | 2003 | 12423921 |